Homology
BLAST of Clc09G23950 vs. NCBI nr
Match:
XP_038888983.1 (taxadiene 5-alpha hydroxylase [Benincasa hispida])
HSP 1 Score: 821.6 bits (2121), Expect = 3.7e-234
Identity = 427/516 (82.75%), Postives = 446/516 (86.43%), Query Frame = 0
Query: 1 MLSFLAAMLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRV 60
MLSF AAMLIISI V+RS KRSKN+PPGQMGLPWIGETMAFY+AQ KNR+FEDFV PRV
Sbjct: 1 MLSFFAAMLIISIAFVIRSKKRSKNVPPGQMGLPWIGETMAFYRAQSKNRLFEDFVEPRV 60
Query: 61 AKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 120
AK+GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQKQGE
Sbjct: 61 AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 120
Query: 121 HRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECL 180
HR VR+LIAASLS+SSLEVMMPKL TI+VYL+TKWVG DTIGLFHSAKVLTFT+VFECL
Sbjct: 121 HRFVRSLIAASLSTSSLEVMMPKLFTTIRVYLDTKWVGRDTIGLFHSAKVLTFTVVFECL 180
Query: 181 MGIKVEPRVLMTFERVLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEGK 240
MGIKVEPRVL TFERVLEGVF PP+ RFSRAKKARLEIEKMLREVVREKRR IEGK
Sbjct: 181 MGIKVEPRVLTTFERVLEGVFAPPIRFPGTRFSRAKKARLEIEKMLREVVREKRRRIEGK 240
Query: 241 LEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHS 300
L EEEEECGSLLSRMVRAMIRGEI+E EVIDNVVL VFAAHDTTSFAIAMTFKMLA H+
Sbjct: 241 L--EEEEECGSLLSRMVRAMIRGEISEMEVIDNVVLFVFAAHDTTSFAIAMTFKMLAHHT 300
Query: 301 NCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360
NCYSLLLQE TDI RSKG +QNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE
Sbjct: 301 NCYSLLLQEQTDIARSKGSNQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360
Query: 361 YERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTHY 420
YERFFIP+GWK VLWTTYGTHY
Sbjct: 361 YERFFIPEGWK---------------------------------------VLWTTYGTHY 420
Query: 421 EEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 480
+EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDW+L
Sbjct: 421 KEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWNL 475
Query: 481 VHPDEPIIMDPLPVPSHGMPIKISPKSEVCPNVHQM 512
VHPDEPIIMDPLPVPS GMP+KIS KS+VCPN HQM
Sbjct: 481 VHPDEPIIMDPLPVPSQGMPMKISLKSDVCPNDHQM 475
BLAST of Clc09G23950 vs. NCBI nr
Match:
XP_008449518.1 (PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo])
HSP 1 Score: 819.3 bits (2115), Expect = 1.8e-233
Identity = 422/517 (81.62%), Postives = 446/517 (86.27%), Query Frame = 0
Query: 1 MLSFLAA-MLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPR 60
MLSFLAA +LII+I VLRSNKRSKN+PPG+MGLPWIGETMAFY+AQ KNR+FEDFV PR
Sbjct: 1 MLSFLAAILLIITIAFVLRSNKRSKNVPPGEMGLPWIGETMAFYRAQSKNRLFEDFVEPR 60
Query: 61 VAKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGE 120
VAK+GKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQK+GE
Sbjct: 61 VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120
Query: 121 RHRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFEC 180
+HR VR LIA++LS+SSLEVMMPKLCNTI+VYL+TKWV CDT+GLF SAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIASALSTSSLEVMMPKLCNTIQVYLDTKWVDCDTVGLFRSAKVLTFTIVFEC 180
Query: 181 LMGIKVEPRVLMTFERVLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEG 240
L+GIKVEPRVL TFERVLEGVF PP RFSRAKKARLEIE MLREVVREKRR IEG
Sbjct: 181 LLGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIETMLREVVREKRRKIEG 240
Query: 241 KLEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQH 300
KL EEEE+CGSLLSRMVRAMI GEI+E EV+DN+VLLVFAAHDTTSFAIAMTFKML+QH
Sbjct: 241 KL--EEEEQCGSLLSRMVRAMIGGEISEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQH 300
Query: 301 SNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI 360
+NCYSLLLQEH DIVRSKGP QNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI
Sbjct: 301 TNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI 360
Query: 361 EYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTH 420
EYE F IPKGWK VLWTTYGTH
Sbjct: 361 EYESFLIPKGWK---------------------------------------VLWTTYGTH 420
Query: 421 YEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWS 480
Y EEYF DPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWS
Sbjct: 421 YGEEYFGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWS 476
Query: 481 LVHPDEPIIMDPLPVPSHGMPIKISPKSEVCPNVHQM 512
LVHPDEPIIMDPLPVP HGMPIKISPKSE+CPNVHQM
Sbjct: 481 LVHPDEPIIMDPLPVPCHGMPIKISPKSEICPNVHQM 476
BLAST of Clc09G23950 vs. NCBI nr
Match:
XP_004140249.1 (taxadiene 5-alpha hydroxylase [Cucumis sativus] >KAE8647348.1 hypothetical protein Csa_003098 [Cucumis sativus])
HSP 1 Score: 812.4 bits (2097), Expect = 2.2e-231
Identity = 419/517 (81.04%), Postives = 443/517 (85.69%), Query Frame = 0
Query: 1 MLSFLAA-MLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPR 60
MLSFLAA +LIISI VLRS KRS+N+PPG MGLPWIGETM FY+AQ KNR+FEDFV PR
Sbjct: 1 MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR 60
Query: 61 VAKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGE 120
VAK+GKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQK+GE
Sbjct: 61 VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120
Query: 121 RHRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFEC 180
+HR VR LIAA+LS+SSL+VMMPKLCNTI+VYL+TKWVGCDTIGLFHSAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 180
Query: 181 LMGIKVEPRVLMTFERVLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEG 240
L+GI+VEPRVL FERVLEGVF PP RFSRAKKARLEIE LREVVREKRR IEG
Sbjct: 181 LLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEG 240
Query: 241 KLEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQH 300
KL EEEECGSLLSRMVRAMI GEI+E EVIDN+VLLVFAAHDTTSFAIAMTFKML+QH
Sbjct: 241 KL---EEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQH 300
Query: 301 SNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI 360
+NCY+LLLQEHTDI+RSKGP QNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI
Sbjct: 301 TNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI 360
Query: 361 EYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTH 420
EYE F IPKGWK VLWTTYGTH
Sbjct: 361 EYESFLIPKGWK---------------------------------------VLWTTYGTH 420
Query: 421 YEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWS 480
Y EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF+H VVTRYDWS
Sbjct: 421 YGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWS 475
Query: 481 LVHPDEPIIMDPLPVPSHGMPIKISPKSEVCPNVHQM 512
LVHPDEPIIMDPLPVP HGMPIKISPKS+VC NVHQM
Sbjct: 481 LVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
BLAST of Clc09G23950 vs. NCBI nr
Match:
KAG7012036.1 (hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 785.0 bits (2026), Expect = 3.8e-223
Identity = 410/516 (79.46%), Postives = 432/516 (83.72%), Query Frame = 0
Query: 1 MLSFLAAMLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRV 60
M FL+A+LIISIV V RS K SK +PPGQMGLPWIGETMAFY+AQRKNR+FE+FV PRV
Sbjct: 13 MFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRV 72
Query: 61 AKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 120
AK+GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQKQGE
Sbjct: 73 AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 132
Query: 121 HRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECL 180
HR R LIAASL+SSSLE MMPKLC TI++YL+TKWVG D I L+HSAKVLTFTIVFECL
Sbjct: 133 HRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECL 192
Query: 181 MGIKVEPRVLMTFERVLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEGK 240
MGIKVEPR+L TFERVLEGVF PP RFSRAKKAR+EI KMLREVVREKRR IE
Sbjct: 193 MGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIE-- 252
Query: 241 LEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHS 300
EE E EEC LLSRMVRAMIRGEI+E EV+DNVVLLVFAAHDTTSFAIA+TFKMLAQ
Sbjct: 253 -EEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQP 312
Query: 301 NCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360
NCYSLLLQEH DIVRSKGP QNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIE
Sbjct: 313 NCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIE 372
Query: 361 YERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTHY 420
YE FFIPKGWK VLWTTYGTH
Sbjct: 373 YEGFFIPKGWK---------------------------------------VLWTTYGTHL 432
Query: 421 EEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 480
++EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL
Sbjct: 433 KDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 486
Query: 481 VHPDEPIIMDPLPVPSHGMPIKISPKSEVCPNVHQM 512
+HPDEPIIMDPLP+PSHGMPIKISPKSE PNV QM
Sbjct: 493 IHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 486
BLAST of Clc09G23950 vs. NCBI nr
Match:
XP_023554746.1 (taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 785.0 bits (2026), Expect = 3.8e-223
Identity = 410/516 (79.46%), Postives = 432/516 (83.72%), Query Frame = 0
Query: 1 MLSFLAAMLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRV 60
M FL+A+LIISI+ V RS K SK +PPGQMGLPWIGETMAFY+AQRKNR+FE+FV PRV
Sbjct: 13 MFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRV 72
Query: 61 AKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 120
AK+GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQKQGE
Sbjct: 73 AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 132
Query: 121 HRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECL 180
HR VR LIAASL+SSSLE MMPKLC TI++YL+TKW+G D I L+HSAKVLTFTIVFECL
Sbjct: 133 HRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECL 192
Query: 181 MGIKVEPRVLMTFERVLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEGK 240
MGIKVEPR+L TFERVLEGVF PP RFSRAKKAR+EI KMLREVVREKRR IE
Sbjct: 193 MGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIE-- 252
Query: 241 LEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHS 300
EE EEEEC LLSRMVRAMIRGEI+E EV+DNVVLLVFAAHDTTSFAIAMTFKMLAQ
Sbjct: 253 -EEPEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQP 312
Query: 301 NCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360
NCYSLLLQEH DIVRSKG QN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIE
Sbjct: 313 NCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIE 372
Query: 361 YERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTHY 420
YE FFIPKGWK VLWTTYGTH
Sbjct: 373 YEGFFIPKGWK---------------------------------------VLWTTYGTHL 432
Query: 421 EEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 480
E+EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL
Sbjct: 433 EDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 486
Query: 481 VHPDEPIIMDPLPVPSHGMPIKISPKSEVCPNVHQM 512
VHPDE IIMDPLP+PSHGMPIKISPKSE PNV QM
Sbjct: 493 VHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 486
BLAST of Clc09G23950 vs. ExPASy Swiss-Prot
Match:
Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)
HSP 1 Score: 307.8 bits (787), Expect = 2.4e-82
Identity = 180/514 (35.02%), Postives = 293/514 (57.00%), Query Frame = 0
Query: 2 LSFLAAMLIISIVLVLRSNKRSK-NIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRV 61
LS +A +L+ ++L+ RS + S +PPG++G+P+IGE+ F +A R N + E F RV
Sbjct: 29 LSAIAGILL--LLLLFRSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSL-EQFFDERV 88
Query: 62 AKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 121
K G +FKTSL+G PTVV+ G NR LSNE KLV SWP+ ++LMGE + ++GE
Sbjct: 89 KKFGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRGED 148
Query: 122 HRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTF---TIVF 181
H ++R+ +A +L+ + K+ I+ ++ KW G D + + + L F I+F
Sbjct: 149 HIVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILF 208
Query: 182 ECLMGIKVEPRVLMTFERVLEGVF-----VPPLRFSRAKKARLEIEKMLREVVREKRRGI 241
+ + + R+ E +L G F +P F RA + R ++ K++ ++++++ +
Sbjct: 209 FNIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKEDL 268
Query: 242 EGKLEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLA 301
+ ++ LL+ R + E++DN L+ A++DTT+ +A+ FK+L+
Sbjct: 269 QSGSATATQDLLSVLLT--FRDDKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLS 328
Query: 302 QHSNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAIS 361
+ CY ++QE +I+ +K + +T +D+K MKYTWQVA+E++R+FPP+FG+FRKAI+
Sbjct: 329 SNPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAIT 388
Query: 362 DIEYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYG 421
DI+Y+ + IPKGWK +LWTTY
Sbjct: 389 DIQYDGYTIPKGWK---------------------------------------LLWTTYS 448
Query: 422 THYEEEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFI-HLVV 481
TH ++ YF +P F PSRF++ V+ Y ++PFGGG R+C G++ +K+ IL+F+ H V
Sbjct: 449 THPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGGQRSCVGWEFSKMEILLFVHHFVK 498
Query: 482 TRYDWSLVHPDEPIIMDPL-PVPSHGMPIKISPK 502
T ++ V PDE I DPL P+PS G IK+ P+
Sbjct: 509 TFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498
BLAST of Clc09G23950 vs. ExPASy Swiss-Prot
Match:
Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)
HSP 1 Score: 304.3 bits (778), Expect = 2.6e-81
Identity = 186/514 (36.19%), Postives = 277/514 (53.89%), Query Frame = 0
Query: 4 FLAAMLIISIVLVLRSNKRSK---NIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRV 63
FLA+ + ++ L SN R+K IPPG G P IGET+ F QRK DF R
Sbjct: 26 FLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRT 85
Query: 64 AKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 123
K+G +F TSL+G PTVV E NRF SNE KLVV+SWPS SV + ++ G+
Sbjct: 86 QKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGDD 145
Query: 124 HRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECL 183
+ +R ++ L +L + ++ + K +L W+G D + K TF++ +
Sbjct: 146 AKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLF 205
Query: 184 MGIKVEP---RVLMTFERVLEGVF-----VPPLRFSRAKKARLEIEKMLREVVREKRRGI 243
I + R+ + F ++GV +P R+++AK A I + L ++ E++ G+
Sbjct: 206 ASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIGL 265
Query: 244 EGKLEEEEEEECGSLLSRMVRAMIRGE-INETEVIDNVVLLVFAAHDTTSFAIAMTFKML 303
E E++ LLS + +GE + + E+ DN++LL++A HDT+S + + K L
Sbjct: 266 EAGNASPEQDLLSFLLSNVDE---QGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFL 325
Query: 304 AQHSNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI 363
A++ +CY +L+E DI SK Q L ED+++MKY+W+VA+E++RLFP GSFRKAI
Sbjct: 326 AENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAI 385
Query: 364 SDIEYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTY 423
+ Y+ F IPKGWK + WT
Sbjct: 386 KEFIYDGFTIPKGWK---------------------------------------MYWTVN 445
Query: 424 GTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTR 483
TH + EYF +P +F PSRFE E Y +VPFGGGPR C G + A+L IL+F+H +V
Sbjct: 446 STHRKSEYFSNPETFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARLEILVFLHNIVKN 495
Query: 484 YDWSLVHPDEPIIMDPLPVPSHGMPIKISPKSEV 505
W+LV+P E +I+DP+P P +G+PIK+ P V
Sbjct: 506 CKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDSV 495
BLAST of Clc09G23950 vs. ExPASy Swiss-Prot
Match:
Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)
HSP 1 Score: 300.8 bits (769), Expect = 2.9e-80
Identity = 184/510 (36.08%), Postives = 277/510 (54.31%), Query Frame = 0
Query: 4 FLAAMLIISIVLVLRSNKRSK---NIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRV 63
FLA+ + ++ L SN R+K IPPG G P IGET+ F QRK DF R
Sbjct: 26 FLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRT 85
Query: 64 AKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 123
K+G +F TSL+G PTVV E NRF SNE KLVV+SWPS SV + ++ G+
Sbjct: 86 QKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGDD 145
Query: 124 HRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECL 183
+ +R ++ L +L + ++ + K +L W+G D + K TF++ +
Sbjct: 146 AKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLF 205
Query: 184 MGIKVE---PRVLMTFERVLEGVF-----VPPLRFSRAKKARLEIEKMLREVVREKRRGI 243
I + R+ + F ++GV +P R+++ K A I + L ++ E++ +
Sbjct: 206 ASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIAL 265
Query: 244 EGKLEEEEEEECGSLLSRMVRAMIRGE-INETEVIDNVVLLVFAAHDTTSFAIAMTFKML 303
E E++ LLS + +GE + + E+ DN++LL++A HDT+S + + K L
Sbjct: 266 EAGNASPEQDLLSFLLSNVDE---QGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFL 325
Query: 304 AQHSNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI 363
A++ +CY +L+E +I SK Q L ED+++MKY+W+VA+E++RLFP + GSFRKAI
Sbjct: 326 AENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAI 385
Query: 364 SDIEYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTY 423
+ Y+ F IPKGWK LH WT
Sbjct: 386 KEFIYDGFTIPKGWK-----------LH----------------------------WTVN 445
Query: 424 GTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTR 483
TH + EYF +P F PSRFE E Y +VPFGGGPR C G + A++ ILIF+H +V
Sbjct: 446 STHQKSEYFSNPEKFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARMEILIFLHNIVKN 491
Query: 484 YDWSLVHPDEPIIMDPLPVPSHGMPIKISP 501
++W+LV+P E +I+DP+P P +G+PIK+ P
Sbjct: 506 FNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491
BLAST of Clc09G23950 vs. ExPASy Swiss-Prot
Match:
Q8W4T9 (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1)
HSP 1 Score: 295.0 bits (754), Expect = 1.6e-78
Identity = 174/514 (33.85%), Postives = 279/514 (54.28%), Query Frame = 0
Query: 4 FLAAMLIISIVLVLRSNKRSK-NIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRVAK 63
F+ ++ I+L RS + S +PPG +G P +GET+ F ++ + + F+ R++K
Sbjct: 15 FVTLAVMAGIILFFRSKRHSSVKLPPGNLGFPLVGETLQFVRSLGSSTP-QQFIEERMSK 74
Query: 64 HGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERHR 123
G +FKTS++G PTVV+ G NR LSNE KLV SWPS ++L+GE+C+ K GE+HR
Sbjct: 75 FGDVFKTSIIGHPTVVLCGPAGNRLVLSNENKLVQMSWPSSMMKLIGEDCLGGKTGEQHR 134
Query: 124 IVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECLMG 183
IVRA + L +L+ K+ + I+ ++ KW G D + K L F++ G
Sbjct: 135 IVRAALTRFLGPQALQNHFAKMSSGIQRHINEKWKGKDEATVLPLVKDLVFSVASRLFFG 194
Query: 184 I---KVEPRVLMTFERVLEGVF-----VPPLRFSRAKKARLEIEKMLREVVREKRRGIEG 243
I ++ ++ E +L G F +P + +A +AR + ++ ++ ++R +
Sbjct: 195 ITEEHLQEQLHNLLEVILVGSFSVPLNIPGFSYHKAIQARATLADIMTHLIEKRRNELRA 254
Query: 244 KLEEEEEEECGSLLSRMVRAMIRG-EINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQ 303
E ++ LL+ RG + + E++DN +L+ ++D+T+ + M K+LA
Sbjct: 255 GTASENQDLLSVLLTFTDE---RGNSLADKEILDNFSMLLHGSYDSTNSPLTMLIKVLAS 314
Query: 304 HSNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISD 363
H Y + QE I+ +K + + +D+K+MKY+WQV +E++R++PPIFG+FRKAI+D
Sbjct: 315 HPESYEKVAQEQFGILSTKMEGEEIAWKDLKEMKYSWQVVQETLRMYPPIFGTFRKAITD 374
Query: 364 IEYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGT 423
I Y + IPKGWK +LWTTY T
Sbjct: 375 IHYNGYTIPKGWK---------------------------------------LLWTTYST 434
Query: 424 HYEEEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTR 483
+EEYF+D FKPSRFEE V+ Y Y+PFGGG R C G++ AK+ L+F+H V
Sbjct: 435 QTKEEYFKDADQFKPSRFEEEGKHVTPYTYLPFGGGMRVCPGWEFAKMETLLFLHHFVKA 485
Query: 484 YD-WSLVHPDEPIIMDPL-PVPSHGMPIKISPKS 503
+ + P+E + PL P+P +G+PIK+ +S
Sbjct: 495 FSGLKAIDPNEKLSGKPLPPLPVNGLPIKLYSRS 485
BLAST of Clc09G23950 vs. ExPASy Swiss-Prot
Match:
Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)
HSP 1 Score: 290.8 bits (743), Expect = 3.0e-77
Identity = 177/510 (34.71%), Postives = 277/510 (54.31%), Query Frame = 0
Query: 7 AMLIISIVLVLRSNKRSK-NIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRVAKHGK 66
A +++ ++L LRS +RS +PPG++G P+IGE++ F KA R N V E F+ RV G
Sbjct: 30 AGIVLPLLLFLRSKRRSSVGLPPGKLGYPFIGESLLFLKALRSNTV-EQFLDERVKNFGN 89
Query: 67 IFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERHRIVR 126
+FKTSL+G PTVV+ G NR L+NE KLV SWP S++LMGE+ I K+GE H I+R
Sbjct: 90 VFKTSLIGHPTVVLCGPAGNRLILANEEKLVQMSWPKSSMKLMGEKSITAKRGEGHMIIR 149
Query: 127 ALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECLMGIKV 186
+ + S +L+ + ++ TI+ ++ KW G D + + L F I I
Sbjct: 150 SALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDISACLFFNINE 209
Query: 187 E---PRVLMTFERVLEGVF-----VPPLRFSRAKKARLEIEKMLREVVREKRRGIEGKLE 246
+ R+ E + GV +P + RA +AR ++ +L ++ +++ + L
Sbjct: 210 KHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEKRKMDLSSGLA 269
Query: 247 EEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHSNC 306
++ L+ + ++ E++DN L+ ++DTT A+A FK+L+ + C
Sbjct: 270 TSNQDLLSVFLT--FKDDRGNPCSDEEILDNFSGLLHGSYDTTVSAMACVFKLLSSNPEC 329
Query: 307 YSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYE 366
Y ++QE I+ +K +T +D+K MKYTWQV +E++RL+P IFGSFR+AI+DI Y
Sbjct: 330 YEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFGSFRQAITDIHYN 389
Query: 367 RFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTHYEE 426
+ IPKGWK +LWT Y TH +E
Sbjct: 390 GYIIPKGWK---------------------------------------LLWTPYTTHPKE 449
Query: 427 EYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFI-HLVVTRYDW 486
YF +P F PSRF++ V+ Y ++PFGGG R+C G++ +K+ IL+ + H V T +
Sbjct: 450 MYFSEPEKFLPSRFDQEGKLVAPYTFLPFGGGQRSCPGWEFSKMEILLSVHHFVKTFSTF 497
Query: 487 SLVHPDEPIIMDPL-PVPSHGMPIKISPKS 503
+ V P E I D L P+PS+G +K+ P+S
Sbjct: 510 TPVDPAEIIARDSLCPLPSNGFSVKLFPRS 497
BLAST of Clc09G23950 vs. ExPASy TrEMBL
Match:
A0A1S3BLK9 (taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1)
HSP 1 Score: 819.3 bits (2115), Expect = 8.9e-234
Identity = 422/517 (81.62%), Postives = 446/517 (86.27%), Query Frame = 0
Query: 1 MLSFLAA-MLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPR 60
MLSFLAA +LII+I VLRSNKRSKN+PPG+MGLPWIGETMAFY+AQ KNR+FEDFV PR
Sbjct: 1 MLSFLAAILLIITIAFVLRSNKRSKNVPPGEMGLPWIGETMAFYRAQSKNRLFEDFVEPR 60
Query: 61 VAKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGE 120
VAK+GKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQK+GE
Sbjct: 61 VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120
Query: 121 RHRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFEC 180
+HR VR LIA++LS+SSLEVMMPKLCNTI+VYL+TKWV CDT+GLF SAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIASALSTSSLEVMMPKLCNTIQVYLDTKWVDCDTVGLFRSAKVLTFTIVFEC 180
Query: 181 LMGIKVEPRVLMTFERVLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEG 240
L+GIKVEPRVL TFERVLEGVF PP RFSRAKKARLEIE MLREVVREKRR IEG
Sbjct: 181 LLGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIETMLREVVREKRRKIEG 240
Query: 241 KLEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQH 300
KL EEEE+CGSLLSRMVRAMI GEI+E EV+DN+VLLVFAAHDTTSFAIAMTFKML+QH
Sbjct: 241 KL--EEEEQCGSLLSRMVRAMIGGEISEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQH 300
Query: 301 SNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI 360
+NCYSLLLQEH DIVRSKGP QNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI
Sbjct: 301 TNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI 360
Query: 361 EYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTH 420
EYE F IPKGWK VLWTTYGTH
Sbjct: 361 EYESFLIPKGWK---------------------------------------VLWTTYGTH 420
Query: 421 YEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWS 480
Y EEYF DPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWS
Sbjct: 421 YGEEYFGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWS 476
Query: 481 LVHPDEPIIMDPLPVPSHGMPIKISPKSEVCPNVHQM 512
LVHPDEPIIMDPLPVP HGMPIKISPKSE+CPNVHQM
Sbjct: 481 LVHPDEPIIMDPLPVPCHGMPIKISPKSEICPNVHQM 476
BLAST of Clc09G23950 vs. ExPASy TrEMBL
Match:
A0A6J1GJY9 (taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)
HSP 1 Score: 779.2 bits (2011), Expect = 1.0e-221
Identity = 407/516 (78.88%), Postives = 430/516 (83.33%), Query Frame = 0
Query: 1 MLSFLAAMLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRV 60
M FL+A+LIISI+ V RS K SK +PPGQMGLPWIGETM FY+AQRKNR+FE+FV PRV
Sbjct: 13 MFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVGPRV 72
Query: 61 AKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 120
AK+GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQKQGE
Sbjct: 73 AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 132
Query: 121 HRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECL 180
HR VR LIAASL+SSSLE MMPKLC TI++YL+TKW+G D I L+HSAKVLTFTIVFECL
Sbjct: 133 HRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECL 192
Query: 181 MGIKVEPRVLMTFERVLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEGK 240
MGIKVEPR+L TFERVLEGVF PP RFSRAKKAR+EI KMLREVVREKRR IE
Sbjct: 193 MGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIE-- 252
Query: 241 LEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHS 300
EE EEEC LLSRMVRAMIRGEI+E EV+DNVVLLVFAAHDTTSFAIAMTFKMLAQ
Sbjct: 253 -EEPVEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQP 312
Query: 301 NCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360
NCYSLLLQEH DIVRSKG QN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIE
Sbjct: 313 NCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIE 372
Query: 361 YERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTHY 420
YE FFIPKGWK VLWTTYGTH
Sbjct: 373 YEGFFIPKGWK---------------------------------------VLWTTYGTHL 432
Query: 421 EEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 480
E++YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL
Sbjct: 433 EDKYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 486
Query: 481 VHPDEPIIMDPLPVPSHGMPIKISPKSEVCPNVHQM 512
VHPDE IIMDPLP+PSHGMPIKISPKSE PNV QM
Sbjct: 493 VHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 486
BLAST of Clc09G23950 vs. ExPASy TrEMBL
Match:
A0A0A0KEB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G423370 PE=3 SV=1)
HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 396/486 (81.48%), Postives = 417/486 (85.80%), Query Frame = 0
Query: 31 MGLPWIGETMAFYKAQRKNRVFEDFVRPRVAKHGKIFKTSLMGSPTVVVNGAEANRFFLS 90
MGLPWIGETM FY+AQ KNR+FEDFV PRVAK+GKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 1 MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
Query: 91 NEFKLVVSSWPSGSVQLMGEECIMQKQGERHRIVRALIAASLSSSSLEVMMPKLCNTIKV 150
NEFKLVVSSWPS SVQLMGEECIMQK+GE+HR VR LIAA+LS+SSL+VMMPKLCNTI+V
Sbjct: 61 NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 120
Query: 151 YLETKWVGCDTIGLFHSAKVLTFTIVFECLMGIKVEPRVLMTFERVLEGVFVPPL----- 210
YL+TKWVGCDTIGLFHSAKVLTFTIVFECL+GI+VEPRVL FERVLEGVF PP
Sbjct: 121 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 180
Query: 211 RFSRAKKARLEIEKMLREVVREKRRGIEGKLEEEEEEECGSLLSRMVRAMIRGEINETEV 270
RFSRAKKARLEIE LREVVREKRR IEGKL EEEECGSLLSRMVRAMI GEI+E EV
Sbjct: 181 RFSRAKKARLEIETTLREVVREKRRKIEGKL---EEEECGSLLSRMVRAMIGGEISEMEV 240
Query: 271 IDNVVLLVFAAHDTTSFAIAMTFKMLAQHSNCYSLLLQEHTDIVRSKGPSQNLTMEDIKK 330
IDN+VLLVFAAHDTTSFAIAMTFKML+QH+NCY+LLLQEHTDI+RSKGP QNLTMEDIKK
Sbjct: 241 IDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKK 300
Query: 331 MKYTWQVARESIRLFPPIFGSFRKAISDIEYERFFIPKGWKVKISINSSAVSLHQLIYPA 390
MKYTWQVARESIRLFPPIFGSFRKAISDIEYE F IPKGWK
Sbjct: 301 MKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWK------------------- 360
Query: 391 VNCCPEMTIILPRIILVAWQVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGG 450
VLWTTYGTHY EEYFEDPLSFKPSRFEEPVSQYVYVPFGG
Sbjct: 361 --------------------VLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGG 420
Query: 451 GPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDEPIIMDPLPVPSHGMPIKISPKSEVC 510
GPRACAGYQLAKLNILIF+H VVTRYDWSLVHPDEPIIMDPLPVP HGMPIKISPKS+VC
Sbjct: 421 GPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVC 444
Query: 511 PNVHQM 512
NVHQM
Sbjct: 481 SNVHQM 444
BLAST of Clc09G23950 vs. ExPASy TrEMBL
Match:
A0A6J1HZM9 (taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468378 PE=3 SV=1)
HSP 1 Score: 772.7 bits (1994), Expect = 9.6e-220
Identity = 404/516 (78.29%), Postives = 430/516 (83.33%), Query Frame = 0
Query: 1 MLSFLAAMLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRV 60
M FL+A+LIISI+ V RS K SK +PPGQMGLPWIG+TMAFY+AQRKNR+FEDFV PRV
Sbjct: 1 MFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGQTMAFYRAQRKNRLFEDFVGPRV 60
Query: 61 AKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 120
AK+GKIFKTSLMGSPTVV+NGAEANRFFLSNEFKLVVSSWP+ SVQLMGEECIMQKQGE
Sbjct: 61 AKYGKIFKTSLMGSPTVVINGAEANRFFLSNEFKLVVSSWPAASVQLMGEECIMQKQGES 120
Query: 121 HRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECL 180
HR VR LIAASL+SSSLE MMPKLC TI+++L+TKWVG D I L+HSAKVLTF+IVFECL
Sbjct: 121 HRCVRGLIAASLASSSLEAMMPKLCTTIQLHLDTKWVGHDIISLYHSAKVLTFSIVFECL 180
Query: 181 MGIKVEPRVLMTFERVLEGVFV-----PPLRFSRAKKARLEIEKMLREVVREKRRGIEGK 240
MGIKVEPR+L TFERVLEGVF P RFSRAKKAR+EI KMLREVVREKRR IE
Sbjct: 181 MGIKVEPRMLTTFERVLEGVFALPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIE-- 240
Query: 241 LEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHS 300
EE EEEEC LLSRMVRAMIRGEI+E EV+DNVVLLVFAAHDTTSFAIAMTFKMLAQH
Sbjct: 241 -EELEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQHP 300
Query: 301 NCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360
+CYSLLLQEH DIVRSKG QNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIE
Sbjct: 301 SCYSLLLQEHADIVRSKGAGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIE 360
Query: 361 YERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTHY 420
YE FFIPKGWK VLWT YGTH
Sbjct: 361 YEGFFIPKGWK---------------------------------------VLWTMYGTHL 420
Query: 421 EEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 480
E+EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL
Sbjct: 421 EDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSL 474
Query: 481 VHPDEPIIMDPLPVPSHGMPIKISPKSEVCPNVHQM 512
+HPDE IIMDPLP+PSHGM KISPKSEV PNV QM
Sbjct: 481 IHPDETIIMDPLPIPSHGMQTKISPKSEVRPNVQQM 474
BLAST of Clc09G23950 vs. ExPASy TrEMBL
Match:
A0A6J1GJT6 (taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)
HSP 1 Score: 766.1 bits (1977), Expect = 8.9e-218
Identity = 398/505 (78.81%), Postives = 420/505 (83.17%), Query Frame = 0
Query: 12 SIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRVAKHGKIFKTSL 71
S++ L S K SK +PPGQMGLPWIGETM FY+AQRKNR+FE+FV PRVAK+GKIFKTSL
Sbjct: 37 SVIASLGSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSL 96
Query: 72 MGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERHRIVRALIAAS 131
MGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQKQGE HR VR LIAAS
Sbjct: 97 MGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAAS 156
Query: 132 LSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECLMGIKVEPRVLM 191
L+SSSLE MMPKLC TI++YL+TKW+G D I L+HSAKVLTFTIVFECLMGIKVEPR+L
Sbjct: 157 LASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLT 216
Query: 192 TFERVLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEGKLEEEEEEECGS 251
TFERVLEGVF PP RFSRAKKAR+EI KMLREVVREKRR IE EE EEEC
Sbjct: 217 TFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIE---EEPVEEECEL 276
Query: 252 LLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHSNCYSLLLQEHT 311
LLSRMVRAMIRGEI+E EV+DNVVLLVFAAHDTTSFAIAMTFKMLAQ NCYSLLLQEH
Sbjct: 277 LLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHA 336
Query: 312 DIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYERFFIPKGWK 371
DIVRSKG QN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIEYE FFIPKGWK
Sbjct: 337 DIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWK 396
Query: 372 VKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTHYEEEYFEDPLSF 431
VLWTTYGTH E++YFEDPLSF
Sbjct: 397 ---------------------------------------VLWTTYGTHLEDKYFEDPLSF 456
Query: 432 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDEPIIMDP 491
KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDE IIMDP
Sbjct: 457 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDP 499
Query: 492 LPVPSHGMPIKISPKSEVCPNVHQM 512
LP+PSHGMPIKISPKSE PNV QM
Sbjct: 517 LPIPSHGMPIKISPKSEARPNVQQM 499
BLAST of Clc09G23950 vs. TAIR 10
Match:
AT2G42850.1 (cytochrome P450, family 718 )
HSP 1 Score: 573.9 bits (1478), Expect = 1.3e-163
Identity = 291/491 (59.27%), Postives = 357/491 (72.71%), Query Frame = 0
Query: 16 VLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRVAKHGKIFKTSLMGSP 75
+ + ++ K + PG+MGLPWIGETM FYKAQ+ NRVFEDFV PR+ KHG IFKT +MGSP
Sbjct: 35 ITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGSP 94
Query: 76 TVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERHRIVRALIAASLSSS 135
T+VVNGAEANR LSNEF LVVSSWPS SVQLMG CIM KQGE+HR++R ++A SLS
Sbjct: 95 TIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSLSYI 154
Query: 136 SLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVFECLMGIKVEPRVLMTFER 195
LE ++PKLC+T+K + ET+W G + I L+ SAKVLTFT+VFECL GIKVE +L FER
Sbjct: 155 GLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGIKVEIGMLEVFER 214
Query: 196 VLEGVFVPPL-----RFSRAKKARLEIEKMLREVVREKRRGIEGKLEEEEEEECGSLLSR 255
VLEGVF P+ +F+RAKKARLEIE L VREKRR +E +E E+ +L SR
Sbjct: 215 VLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREME---KEGAEKPNTTLFSR 274
Query: 256 MVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHSNCYSLLLQEHTDIVR 315
+V +I+G I E EV+DN+VLLVFAAHDTTS+A++MTFKMLAQH C LLQEH I
Sbjct: 275 LVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKA 334
Query: 316 SKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYERFFIPKGWKVKIS 375
+KG + LT+ED+KKMKY+WQV RE++RL PPIFGSFRKA++DI+Y + IPKGWK
Sbjct: 335 NKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWK---- 394
Query: 376 INSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLWTTYGTHYEEEYFEDPLSFKPSR 435
+LWTTYGTHY E F+DP+SF P+R
Sbjct: 395 -----------------------------------ILWTTYGTHYNPEIFQDPMSFDPTR 454
Query: 436 FEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDEPIIMDPLPVP 495
F++P+ Y Y+PFGGGPR CAG+QLAK++IL+F+H VVT +DWSLV+PDE I MDPLP P
Sbjct: 455 FDKPIQAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFP 483
Query: 496 SHGMPIKISPK 502
S GMPIKISPK
Sbjct: 515 SLGMPIKISPK 483
BLAST of Clc09G23950 vs. TAIR 10
Match:
AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )
HSP 1 Score: 273.9 bits (699), Expect = 2.7e-73
Identity = 176/520 (33.85%), Postives = 268/520 (51.54%), Query Frame = 0
Query: 1 MLSFLAAMLIISIVLVLR---SNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVR 60
M+ + +++S++L+LR S+ N+PPG GLP IGE+ +F A R+ E F+
Sbjct: 6 MIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHP-EKFIT 65
Query: 61 PRVAKHGK----IFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECI 120
RV + +FKT L GSPT VV GA N+F +NE KLVVS WP SV + +
Sbjct: 66 DRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPD-SVNKIFPSSM 125
Query: 121 MQKQGERHRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTF 180
E R +R L++ + +L + + + + ET+W D + +F K TF
Sbjct: 126 QTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFTF 185
Query: 181 TIVFECLMGIKVEPRVLM---TFERVLEGVF-----VPPLRFSRAKKARLEIEKMLREVV 240
+I + ++ RV F V G+F +P RF+RA KA + K + +V
Sbjct: 186 SIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAIV 245
Query: 241 REKRRGIE-GKLEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAI 300
R+++ ++ GK EE + +LS M+ M GE + ++ D ++ L+ HDT S
Sbjct: 246 RQRKEELKAGKALEEHD-----ILSHML--MNIGETKDEDLADKIIGLLIGGHDTASIVC 305
Query: 301 AMTFKMLAQHSNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIF 360
LA+ + Y +LQE +I++ K + L EDI+KM+Y+W VA E +R+ PP+
Sbjct: 306 TFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLS 365
Query: 361 GSFRKAISDIEYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAW 420
G+FR+AI ++ F+IPKGWK
Sbjct: 366 GTFREAIDHFSFKGFYIPKGWK-------------------------------------- 425
Query: 421 QVLWTTYGTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIF 480
+ W+ TH +YF +P F+P+RFE Y YVPFGGGPR C G + A+L ILIF
Sbjct: 426 -LYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPFGGGPRMCPGKEYARLEILIF 477
Query: 481 IHLVVTRYDWSLVHPDE-PIIMDPLPVPSHGMPIKISPKS 503
+H +V R+ W V P+E I++DPLP+P G+PI+I P+S
Sbjct: 486 MHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQS 477
BLAST of Clc09G23950 vs. TAIR 10
Match:
AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 218.4 bits (555), Expect = 1.3e-56
Identity = 149/513 (29.04%), Postives = 250/513 (48.73%), Query Frame = 0
Query: 2 LSFLAAMLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRVA 61
L+ + ++++ L ++ +PPG MGLP+IGET+ Y + F R
Sbjct: 25 LTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FFATRQN 84
Query: 62 KHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERH 121
K+G IFKT ++G P V+++ EA R L ++ L ++P +++G E + QG H
Sbjct: 85 KYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYH 144
Query: 122 RIVRALIAASLSSSSLEVMMPKLCNTIKVYLET--KWVGCDTIGLFHSAKVLTFTIVFEC 181
++ L+ +S S+L P + + + L+T W +I K F +
Sbjct: 145 STLKRLVQSSFMPSALR---PTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMS 204
Query: 182 LMGIKVEPRVL----MTFERVLEG-----VFVPPLRFSRAKKARLEIEKMLREVVREKRR 241
G K EP + + ++R+ G + +P F ++ KAR+E+ + LR+V+ EKRR
Sbjct: 205 AFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI-EKRR 264
Query: 242 GIEGKLEEEEEEECGSLLSRMVRA--MIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTF 301
E E G LL ++ A R ++++++ DN++ ++FAA DTT+ +
Sbjct: 265 --------ENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLL 324
Query: 302 KMLAQHSNCYSLLLQEHTDI-VRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSF 361
K L H N + +E I + K ++ ++ ED +KM T +V +E++R + +F
Sbjct: 325 KYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTF 384
Query: 362 RKAISDIEYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVL 421
R+A+ D+EY+ + IPKGWKV LP +
Sbjct: 385 REAVQDVEYDGYLIPKGWKV----------------------------LPLFRRI----- 444
Query: 422 WTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLV 481
H+ E+F DP F PSRFE Y Y+PFG G +C G +LAKL +LI +H +
Sbjct: 445 ------HHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHL 481
Query: 482 VTRYDWSLVHPDEPIIMDPLPVPSHGMPIKISP 501
T + W ++ +E I P PVP G+PI+++P
Sbjct: 505 TTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481
BLAST of Clc09G23950 vs. TAIR 10
Match:
AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 218.4 bits (555), Expect = 1.3e-56
Identity = 149/513 (29.04%), Postives = 250/513 (48.73%), Query Frame = 0
Query: 2 LSFLAAMLIISIVLVLRSNKRSKNIPPGQMGLPWIGETMAFYKAQRKNRVFEDFVRPRVA 61
L+ + ++++ L ++ +PPG MGLP+IGET+ Y + F R
Sbjct: 25 LTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FFATRQN 84
Query: 62 KHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERH 121
K+G IFKT ++G P V+++ EA R L ++ L ++P +++G E + QG H
Sbjct: 85 KYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYH 144
Query: 122 RIVRALIAASLSSSSLEVMMPKLCNTIKVYLET--KWVGCDTIGLFHSAKVLTFTIVFEC 181
++ L+ +S S+L P + + + L+T W +I K F +
Sbjct: 145 STLKRLVQSSFMPSALR---PTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMS 204
Query: 182 LMGIKVEPRVL----MTFERVLEG-----VFVPPLRFSRAKKARLEIEKMLREVVREKRR 241
G K EP + + ++R+ G + +P F ++ KAR+E+ + LR+V+ EKRR
Sbjct: 205 AFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI-EKRR 264
Query: 242 GIEGKLEEEEEEECGSLLSRMVRA--MIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTF 301
E E G LL ++ A R ++++++ DN++ ++FAA DTT+ +
Sbjct: 265 --------ENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLL 324
Query: 302 KMLAQHSNCYSLLLQEHTDI-VRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSF 361
K L H N + +E I + K ++ ++ ED +KM T +V +E++R + +F
Sbjct: 325 KYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTF 384
Query: 362 RKAISDIEYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVL 421
R+A+ D+EY+ + IPKGWKV LP +
Sbjct: 385 REAVQDVEYDGYLIPKGWKV----------------------------LPLFRRI----- 444
Query: 422 WTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLV 481
H+ E+F DP F PSRFE Y Y+PFG G +C G +LAKL +LI +H +
Sbjct: 445 ------HHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHL 481
Query: 482 VTRYDWSLVHPDEPIIMDPLPVPSHGMPIKISP 501
T + W ++ +E I P PVP G+PI+++P
Sbjct: 505 TTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481
BLAST of Clc09G23950 vs. TAIR 10
Match:
AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )
HSP 1 Score: 202.2 bits (513), Expect = 9.9e-52
Identity = 144/516 (27.91%), Postives = 248/516 (48.06%), Query Frame = 0
Query: 2 LSFLAAMLIISIVLVLRSNKRSKN----IPPGQMGLPWIGETMAFYKAQRKNRVFEDFVR 61
L+ A L + + L S +R + +PPG MG P++GET Y +Q N F+
Sbjct: 8 LTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY-SQDPNVFFQS--- 67
Query: 62 PRVAKHGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQ 121
+ ++G +FKT ++G P V+++ EA +F L + L ++P+ +++G++ I Q
Sbjct: 68 -KQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQ 127
Query: 122 GERHRIVRALIAASLSSSSLEVMMPKLCNTIKVYLETKWVGCDTIGLFHSAKVLTFTIVF 181
G+ H +R L+ + S+ M+P + + + L + W G I + K TF +
Sbjct: 128 GDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRS-WEG-TMINTYQEMKTYTFNVAL 187
Query: 182 ECLMGIKVEPRVLMTFER---VLE------GVFVPPLRFSRAKKARLEIEKMLREVVREK 241
+ G K E +R +LE V +P F ++ KAR E+ ++L ++ E+
Sbjct: 188 LSIFG-KDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSER 247
Query: 242 RRGIEGKLEEEEEEECGSLLSRMVRAMIRGEINETEVIDNVVLLVFAAHDTTSFAIAMTF 301
R+ + GS + + E+ + ++ DN++ ++FAA DTT+ ++
Sbjct: 248 RQN-----GSSHNDLLGSFMGD------KEELTDEQIADNIIGVIFAARDTTASVMSWIL 307
Query: 302 KMLAQHSNCYSLLLQEHTDIVRSKGPSQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFR 361
K LA++ N + +E I + K ++LT D KKM T +V +E++R+ + +FR
Sbjct: 308 KYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFR 367
Query: 362 KAISDIEYERFFIPKGWKVKISINSSAVSLHQLIYPAVNCCPEMTIILPRIILVAWQVLW 421
+A+ D+EYE + IPKGWKV LP
Sbjct: 368 EAVEDVEYEGYLIPKGWKV----------------------------LPLF--------- 427
Query: 422 TTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVV 481
H+ + F +P F PSRFE ++PFG G +C G +LAKL + I IH +
Sbjct: 428 --RNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLT 465
Query: 482 TRYDWSLVHPDEPIIMDPLPVPSHGMPIKISPKSEV 505
T+Y WS+V + I P +P +G+PI ++ K E+
Sbjct: 488 TKYSWSIVGASDGIQYGPFALPQNGLPIVLARKPEI 465
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038888983.1 | 3.7e-234 | 82.75 | taxadiene 5-alpha hydroxylase [Benincasa hispida] | [more] |
XP_008449518.1 | 1.8e-233 | 81.62 | PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo] | [more] |
XP_004140249.1 | 2.2e-231 | 81.04 | taxadiene 5-alpha hydroxylase [Cucumis sativus] >KAE8647348.1 hypothetical prote... | [more] |
KAG7012036.1 | 3.8e-223 | 79.46 | hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyro... | [more] |
XP_023554746.1 | 3.8e-223 | 79.46 | taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q6WG30 | 2.4e-82 | 35.02 | Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2 | [more] |
Q50EK0 | 2.6e-81 | 36.19 | Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1 | [more] |
Q50EK1 | 2.9e-80 | 36.08 | Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1 | [more] |
Q8W4T9 | 1.6e-78 | 33.85 | Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1 | [more] |
Q84KI1 | 3.0e-77 | 34.71 | Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BLK9 | 8.9e-234 | 81.62 | taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1 | [more] |
A0A6J1GJY9 | 1.0e-221 | 78.88 | taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
A0A0A0KEB2 | 1.1e-220 | 81.48 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G423370 PE=3 SV=1 | [more] |
A0A6J1HZM9 | 9.6e-220 | 78.29 | taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146... | [more] |
A0A6J1GJT6 | 8.9e-218 | 78.81 | taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
Match Name | E-value | Identity | Description | |
AT2G42850.1 | 1.3e-163 | 59.27 | cytochrome P450, family 718 | [more] |
AT5G36110.1 | 2.7e-73 | 33.85 | cytochrome P450, family 716, subfamily A, polypeptide 1 | [more] |
AT2G29090.1 | 1.3e-56 | 29.04 | cytochrome P450, family 707, subfamily A, polypeptide 2 | [more] |
AT2G29090.2 | 1.3e-56 | 29.04 | cytochrome P450, family 707, subfamily A, polypeptide 2 | [more] |
AT4G19230.1 | 9.9e-52 | 27.91 | cytochrome P450, family 707, subfamily A, polypeptide 1 | [more] |