Clc09G09150 (gene) Watermelon (cordophanus) v2

BLAST of Clc09G09150 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 582/719 (80.95%), Postives = 638/719 (88.73%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLTK 72
           T IVHVEQPKIRLVGESSND IESWYMSF+P +T +T E P+LLYSYRNVMSGFSARLT 
Sbjct: 36  TYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTI 95

Query: 73  EQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGIH 132
           EQVKAM+KKDGFISAMPETIM+LHTTHTP+Y GLNQ  G+WKNSN GKGVI+GVLDTGIH
Sbjct: 96  EQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIH 155

Query: 133 PSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGTH 192
           P+HPSF+DEGMSPPPAKWKG+CEFN S                      SP+DEKGHGTH
Sbjct: 156 PNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTH 215

Query: 193 TASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDGV 252
           TAST+AGAFVE A ALGNA+GKAAG+APLAHLAIYKVCS K CPSSDV AGIDAAIDDGV
Sbjct: 216 TASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGV 275

Query: 253 DVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGAS 312
           DVLSISLG+ SVPFF+D+IAVATF AIQKGIFVS SAGNSGP +STLSN+APWILTVGAS
Sbjct: 276 DVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGAS 335

Query: 313 TLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS------------SLENMDV 372
           T+NRRIVAVAKLGNGE+Y+GESLYQP+D  S FLPL YAG+            SLENMDV
Sbjct: 336 TINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDV 395

Query: 373 KGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAGV 432
           KGKV+VCE KGGVG+VAKG+VVKNAGGAAMILINQ++DGFST S+AHVLPA HVSY+AGV
Sbjct: 396 KGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGV 455

Query: 433 LIKSYINSSQKPTASISFEGTIIGD-DEFSAPAMASFSSRGPCLPSPGILKPDIIGPGVN 492
           LIKSYINSSQ PTASISF+GT+IGD D+FSAP+MASFSSRGPCLPSPGILKPDI GPGVN
Sbjct: 456 LIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVN 515

Query: 493 ILAAWPFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN 552
           ILAAWPFPLDN+TNT  TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN
Sbjct: 516 ILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN 575

Query: 553 IKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEQVS 612
           IKTP+G+PI+DQDL+ ANFFAMG+GHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE+VS
Sbjct: 576 IKTPQGEPIVDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVS 635

Query: 613 IIVHKTVICGLLPRIPEGDLNYPSFSVALGELQTFKRTVTNVGEANSVYTAIVEAPVGVS 672
           IIVH+TVICGL+P I EGDLNYPSFSVALG LQTFKRTVTNVGEANSVYTAIVEAP+GVS
Sbjct: 636 IIVHRTVICGLVPSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVS 695

Query: 673 MKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWV--SEKHVVGSPIVVKF 695
           M V PRKL FSR+NQ +TF+VTF+RI  V+IVGEFG+GYLKWV  S+K+VV SP+ VKF
Sbjct: 696 MTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 754

BLAST of Clc09G09150 vs. NCBI nr
Match: XP_008437722.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 581/719 (80.81%), Postives = 636/719 (88.46%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLTK 72
           T IVHVEQPKIRLVGESSND IESWYMSF+P +T +T E P+LLYSYRNVMSGFSARLT 
Sbjct: 45  TYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTI 104

Query: 73  EQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGIH 132
           EQVKAM+KKDGFISAMPETIM+LHTTHTP+Y GLNQ  G+WKNSN GKGVI+GVLDTGIH
Sbjct: 105 EQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIH 164

Query: 133 PSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGTH 192
           P+HPSF+DEGMSPPPAKWKG+CEFN S                      SP+DEKGHGTH
Sbjct: 165 PNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTH 224

Query: 193 TASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDGV 252
           TAST+AGAFVE A ALGNA+GKAAG+APLAHLAIYKVCS K CPSSDV AGIDAAIDDGV
Sbjct: 225 TASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGV 284

Query: 253 DVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGAS 312
           DVLSISLG+ SVPFF+D+IAVATF AIQKGIFVS SAGNSGP +STLSN+APWILTVGAS
Sbjct: 285 DVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGAS 344

Query: 313 TLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS------------SLENMDV 372
           T+NRRIVAVAKLGNGE+Y+GESLYQP+D  S FLPL YAG+            SLENMDV
Sbjct: 345 TINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDV 404

Query: 373 KGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAGV 432
           KGKV+VCE KGGVG+VAKG+VVKNAGGAAMILINQ++DGFST S+AHVLPA HVSY+AGV
Sbjct: 405 KGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGV 464

Query: 433 LIKSYINSSQKPTASISFEGTIIGD-DEFSAPAMASFSSRGPCLPSPGILKPDIIGPGVN 492
           LIKSYINSSQ PTASISF+GT+IGD D+FSAP+MASFSSRGPCLPSPGILKPDI GPGVN
Sbjct: 465 LIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVN 524

Query: 493 ILAAWPFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN 552
           ILAAWPFPLDN+TNT  TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN
Sbjct: 525 ILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN 584

Query: 553 IKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEQVS 612
           IKTP+G+PI DQDL+ ANFFAMG+GHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE+VS
Sbjct: 585 IKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVS 644

Query: 613 IIVHKTVICGLLPRIPEGDLNYPSFSVALGELQTFKRTVTNVGEANSVYTAIVEAPVGVS 672
           IIVH+TVICGL+  I EGDLNYPSFSVALG LQTFKRTVTNVGEANSVYTAIVEAP+GVS
Sbjct: 645 IIVHRTVICGLVLSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVS 704

Query: 673 MKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWV--SEKHVVGSPIVVKF 695
           M V PRKL FSR+NQ +TF+VTF+RI  V+IVGEFG+GYLKWV  S+K+VV SP+ VKF
Sbjct: 705 MTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 763

BLAST of Clc09G09150 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 989.2 bits (2556), Expect = 1.8e-284
Identity = 507/720 (70.42%), Postives = 584/720 (81.11%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPAT-TASTTEPPRLLYSYRNVMSGFSARLT 72
           T IVHV+QP + ++G++   D+++WY SFLP T  AS+ E PRLLYSYR+VMSGFSARLT
Sbjct: 35  TYIVHVKQPVLEILGDTI--DLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLT 94

Query: 73  KEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGI 132
           KEQVKAM++KDGFISAMPET +NLHTTHTP+Y GLN+  GLWKNSN GKGVIIGVLDTGI
Sbjct: 95  KEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGI 154

Query: 133 HPSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGT 192
           HP+HPSF+DEGMS PPAKWKGRCEF  S                      SP+DE GHGT
Sbjct: 155 HPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHGT 214

Query: 193 HTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDG 252
           HTAST+AG FV+GA ALGNARGKA GMAPLAH+A+YKVCS   C SSD+LA +DAAIDDG
Sbjct: 215 HTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDG 274

Query: 253 VDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGA 312
           VDVLS+SLGA S PFF D IA+  FAAI+KGIFVSCSAGNSGP  +TL+NEAPWILTVGA
Sbjct: 275 VDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGA 334

Query: 313 STLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAG------------SSLENMD 372
           ST++R+IVA+AKLG+GE Y GESL+QP +  S FLPL YAG             SLE ++
Sbjct: 335 STIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLN 394

Query: 373 VKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAG 432
           V GKV+VCE  GG+ +VAKG+VVKN GGAAMILINQ+ +GFST ++AHVLP  H+SYE G
Sbjct: 395 VTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDG 454

Query: 433 VLIKSYINSSQKPTASISFEGTIIGDDEFS-APAMASFSSRGPCLPSPGILKPDIIGPGV 492
           + IK+YINSS  P ASISF+GT++G+   + +PAMASFSSRGPC  SPGILKPDI GPGV
Sbjct: 455 LKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGV 514

Query: 493 NILAAWPFPLDNNTNTNL--TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 552
           NILAAWPFPL+NNTNTN   TFNVISGTSMSCPHLSGIAALIKS HPNWSPAAIKSAIMT
Sbjct: 515 NILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMT 574

Query: 553 TANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 612
           +A+++ P+GKPI+DQDL+ ANFFAMGSGHVNPSKAA+PGLVYDIQPDDYIPYLC LY D 
Sbjct: 575 SADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDA 634

Query: 613 QVSIIVHKTVICGLLPRIPEGDLNYPSFSVALGE-LQTFKRTVTNVGEANSVYTAIVEAP 672
           QVSIIV K V C  +PRI EGDLNYPSF+V+LG   QTF RTVTNVG+ANSVY  IVEAP
Sbjct: 635 QVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVEAP 694

Query: 673 VGVSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPIVVK 694
            GVS+KVTP  L FS+LN+KVT+SVTFSRI  V+   EF +GYL WVS+K +V SPI VK
Sbjct: 695 AGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 752

BLAST of Clc09G09150 vs. NCBI nr
Match: XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])

HSP 1 Score: 986.5 bits (2549), Expect = 1.2e-283
Identity = 500/720 (69.44%), Postives = 585/720 (81.25%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPAT-TASTTEPPRLLYSYRNVMSGFSARLT 72
           T IVHV+QP++ ++G++   D+++WY SFLP T  AS+ E  RLLYSYR+V+SGFSARLT
Sbjct: 35  TYIVHVKQPEVEILGDTI--DLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSARLT 94

Query: 73  KEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGI 132
           KEQVK M++KDGFISAMPET +NLHTTHTP+Y GLNQ  GLWKNSN GKGVIIGVLDTGI
Sbjct: 95  KEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGI 154

Query: 133 HPSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGT 192
           HP+HPSF+DEGM  PPAKWKGRCEF  S                      SP+DE GHGT
Sbjct: 155 HPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKSPNDENGHGT 214

Query: 193 HTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDG 252
           HTAST+AG FV+GA ALGNARGKA GMAPLAH+A+YKVCS K C SSD+LA +DAAIDDG
Sbjct: 215 HTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDAAIDDG 274

Query: 253 VDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGA 312
           VDVLS+SLGA S PFF+D IAV  FAAI+KGIFVSCSAGNSGP  +TL+NEAPWILTVGA
Sbjct: 275 VDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGA 334

Query: 313 STLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAG------------SSLENMD 372
           ST++R+IVA+AKL +G+ + GESL+QP D +S FLPL YAG             SLE ++
Sbjct: 335 STIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLN 394

Query: 373 VKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAG 432
           V GK++VCE  GG+G++AKG+VVKN GGAAMIL+NQ+ DGFST ++AHVLP  H+SYE G
Sbjct: 395 VTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHLSYEDG 454

Query: 433 VLIKSYINSSQKPTASISFEGTIIGDDEFS-APAMASFSSRGPCLPSPGILKPDIIGPGV 492
           + IK YINSS  P ASISFEGT++G+   + +PAMASFSSRGPC  SPGILKPDI GPGV
Sbjct: 455 LKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGV 514

Query: 493 NILAAWPFPLDNNTNTNL--TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 552
           NILAAWPFPL+NNTNTN   TFNVISGTSMSCPHLSGIAALIKS HPNWSPAAIKSAIMT
Sbjct: 515 NILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPAAIKSAIMT 574

Query: 553 TANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 612
           +A+++ P+GKPI+DQDL+ ANFFAMGSGHVNPSKAA+PGLVYDIQPDDY+PYLC LY D 
Sbjct: 575 SADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDA 634

Query: 613 QVSIIVHKTVICGLLPRIPEGDLNYPSFSVALG-ELQTFKRTVTNVGEANSVYTAIVEAP 672
           QVSIIV + V C  + RI EGDLNYPSF+V+LG + Q F RTVTNVG+ANSVY AIV+AP
Sbjct: 635 QVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVKAP 694

Query: 673 VGVSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPIVVK 694
            GVS++VTPR L FS+LN+K+T+SVTFSRI  V+   EF +GYL WVS KH+V SPI VK
Sbjct: 695 AGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISVK 752

BLAST of Clc09G09150 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 986.5 bits (2549), Expect = 1.2e-283
Identity = 506/720 (70.28%), Postives = 582/720 (80.83%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPAT-TASTTEPPRLLYSYRNVMSGFSARLT 72
           T IVHV+QP + ++G++   D+++WY SFLP T  AS+ E PRLLYSYR+VMSGFSARLT
Sbjct: 15  TYIVHVKQPVLEILGDTI--DLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLT 74

Query: 73  KEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGI 132
           KEQVKAM++KDGFISAMPET +NLHTTHTP+Y GLN   GLWKNSN GKGVIIGVLDTGI
Sbjct: 75  KEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGI 134

Query: 133 HPSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGT 192
           HP+HPSF+DEGMS PPAKWKGRCEF  S                      SP+DE GHGT
Sbjct: 135 HPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHGT 194

Query: 193 HTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDG 252
           HTAST+AG FV+GA ALGNARGKA GMAPLAH+A+YKVCS   C SSD+LA +DAAIDDG
Sbjct: 195 HTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDG 254

Query: 253 VDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGA 312
           VDVLS+SLGA S PFF D IA+  FAAI+KGIFVSCSAGNSGP  +TL+NEAPWILTVGA
Sbjct: 255 VDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGA 314

Query: 313 STLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAG------------SSLENMD 372
           ST++R+IVA+AKLG+GE Y GESL+QP +  S FLPL YAG             SLE ++
Sbjct: 315 STIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLN 374

Query: 373 VKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAG 432
           V GKV+VCE  GG+ +VAKG+VVKN GGAAMILINQ+ +GFST ++AHVLP  H+SYE G
Sbjct: 375 VTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDG 434

Query: 433 VLIKSYINSSQKPTASISFEGTIIGDDEFS-APAMASFSSRGPCLPSPGILKPDIIGPGV 492
           + IK+YINSS  P ASISF+GT++G+   + +PAMASFSSRGPC  SPGILKPDI GPGV
Sbjct: 435 LKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGV 494

Query: 493 NILAAWPFPLDNNTNTNL--TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 552
           NILAAWPFPL+NNTNTN   TFNVISGTSMSCPHLSGIAALIKS HPNWSPAAIKSAIMT
Sbjct: 495 NILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMT 554

Query: 553 TANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 612
           +A+++ P+GKPI+DQDL+ ANFFAMGSGHVNPSKAA+PGLVYDIQPDDYIPYLC LY D 
Sbjct: 555 SADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDA 614

Query: 613 QVSIIVHKTVICGLLPRIPEGDLNYPSFSVAL-GELQTFKRTVTNVGEANSVYTAIVEAP 672
           QVSIIV K V C  +PRI EGDLNYPSF+V+L    QTF RTVTNVG+ANSVY  IVEAP
Sbjct: 615 QVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVEAP 674

Query: 673 VGVSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPIVVK 694
            GVS+KVTP  L FS+LN+KVT+SVTFSRI  V+   EF +GYL WVS+K +V SPI VK
Sbjct: 675 AGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 732

BLAST of Clc09G09150 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 7.8e-246
Identity = 437/719 (60.78%), Postives = 536/719 (74.55%), Query Frame = 0

Query: 15  IVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLTKEQ 74
           I+HV  P+ +++ ES  +D+ESWY SFLP T  S+ E PR++YSY+NV+ GF+A LT+E+
Sbjct: 42  IIHVTGPEGKMLTES--EDLESWYHSFLPPTLMSSEEQPRVIYSYKNVLRGFAASLTQEE 101

Query: 75  VKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGIHPS 134
           + A++KK+GFISA P+ +++  TTHTP + GL Q  G+WK SN GKGVIIGVLD+GI P 
Sbjct: 102 LSAVEKKNGFISAHPQRVLHRQTTHTPKFLGLQQDTGVWKESNFGKGVIIGVLDSGITPG 161

Query: 135 HPSFSDEGMSPPPAKWKGRCEFNIS-------------------------SPSDEKGHGT 194
           HPSFSD G+ PPP KWKGRC+ N++                         +P DE GHGT
Sbjct: 162 HPSFSDVGIPPPPPKWKGRCDLNVTACNNKLIGARAFNLAAEAMNGKKAEAPIDEDGHGT 221

Query: 195 HTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDG 254
           HTAST+AGAFV  A  LGNA+G AAGMAP AHLAIYKVC  ++CP SD+LA +DAA++DG
Sbjct: 222 HTASTAAGAFVNYAEVLGNAKGTAAGMAPHAHLAIYKVCFGEDCPESDILAALDAAVEDG 281

Query: 255 VDVLSISLG-ADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVG 314
           VDV+SISLG ++  PFF D  A+  FAA+QKGIFVSC+AGNSGPF+S++ N APWILTVG
Sbjct: 282 VDVISISLGLSEPPPFFNDSTAIGAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVG 341

Query: 315 ASTLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAG------------SSLENM 374
           AST++RRIVA AKLGNG+E+DGES++QPS    T LPLAYAG             SL++ 
Sbjct: 342 ASTIDRRIVATAKLGNGQEFDGESVFQPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDS 401

Query: 375 DVKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEA 434
             +GKV++CE  GG+ ++AKG  VK AGGAAMIL+N + + FS  +  H LPA HVSY A
Sbjct: 402 AFRGKVVLCERGGGIARIAKGEEVKRAGGAAMILMNDETNAFSLSADVHALPATHVSYAA 461

Query: 435 GVLIKSYINSSQKPTASISFEGTIIGDDEFSAPAMASFSSRGPCLPSPGILKPDIIGPGV 494
           G+ IK+YINS+  PTA+I F+GT+IG+    APA+ASFSSRGP LPSPGILKPDIIGPGV
Sbjct: 462 GIEIKAYINSTATPTATILFKGTVIGNS--LAPAVASFSSRGPNLPSPGILKPDIIGPGV 521

Query: 495 NILAAWPFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTA 554
           NILAAWPFPL N+T++ LTFN+ SGTSMSCPHLSGIAAL+KS HP+WSPAAIKSAIMT+A
Sbjct: 522 NILAAWPFPLSNSTDSKLTFNIESGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMTSA 581

Query: 555 NIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YKDEQ 614
           +      K I+D+ L+  + FA GSGHVNPS+A DPGLVYDIQPDDYIPYLCGL Y + +
Sbjct: 582 DTINLGNKLIVDETLQPTDLFATGSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSETE 641

Query: 615 VSIIVHKTVICGLLPRIPEGDLNYPSFSVALGELQTFKRTVTNVGEANSVYTAIVEAPVG 674
           V II H+ + C     IPEG+LNYPSFSV LG  +TF RTVTNVGEA+S Y  IV AP G
Sbjct: 642 VGIIAHRKIKCS--ASIPEGELNYPSFSVELGSSKTFTRTVTNVGEAHSSYDLIVAAPQG 701

Query: 675 VSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPIVVKF 695
           V +KV P KL FS +NQK T+SVTFSR        E+ QG+LKWVS KH V SPI VKF
Sbjct: 702 VDVKVQPYKLNFSEVNQKETYSVTFSRTGLGNKTQEYAQGFLKWVSTKHTVRSPISVKF 754

BLAST of Clc09G09150 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 1.2e-225
Identity = 409/713 (57.36%), Postives = 519/713 (72.79%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLTK 72
           T IVHV++P  +++  S+  D+ES+Y SFLPAT + +  P R+++SY +V +GF+A+L+ 
Sbjct: 28  TYIVHVDKPDAQVLANSA--DLESYYNSFLPATVSGSEVPSRIIHSYHHVATGFAAKLSG 87

Query: 73  EQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGIH 132
           E+VK M+KK GF+SA  E ++ LHTTHTP++ GL Q +G W+ SN GKGVIIG+LDTGI 
Sbjct: 88  EEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMGFWQESNYGKGVIIGLLDTGIT 147

Query: 133 PSHPSFSDEGMSPPPAKWKGRCEFNISS-------------------PSDEKGHGTHTAS 192
           P HPSFSD  M  PPAKWKG+CEF  ++                   P+DE+GHGTHTAS
Sbjct: 148 PGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARNFISGSGVPPTDEEGHGTHTAS 207

Query: 193 TSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDGVDVL 252
           T+AG FV  AN  GNA G A GMAPLAH+A+YKVCS   C  +D+LA +DAAIDDGVDVL
Sbjct: 208 TAAGNFVNDANVFGNANGTAVGMAPLAHIAMYKVCSEDGCSDADILAALDAAIDDGVDVL 267

Query: 253 SISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGASTLN 312
           S+SLG  S PF+ D+IA+  FAAI+KGIFVS SAGN GP +STLSNEAPWILTVGAST +
Sbjct: 268 SLSLGGYSDPFYYDNIAIGAFAAIRKGIFVSASAGNDGPLNSTLSNEAPWILTVGASTHD 327

Query: 313 RRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS-----------SLENMDVKGKV 372
           R+IVA A LGNG++YDGES +QP+D   T LPL Y G+           SL+  DVKGKV
Sbjct: 328 RKIVATAVLGNGQQYDGESAFQPADFPHTLLPLVYPGTSDEEAAFCSSGSLDKFDVKGKV 387

Query: 373 IVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAGVLIKS 432
           +VC+  G V ++ K   VK+AGGAAMIL N + DG  T + AHVLPA HV Y AG +IKS
Sbjct: 388 VVCDRGGDVARLEKSQTVKDAGGAAMILANLEIDGEGTFADAHVLPATHVGYAAGEMIKS 447

Query: 433 YINSSQKPTASISFEGTIIGDDEFSAPAMASFSSRGPCLPSPGILKPDIIGPGVNILAAW 492
           YINS+  PTA I F+GTIIG    S+P+++SFSSRGP L SPGI+KPDIIGPGVNILAAW
Sbjct: 448 YINSTSTPTAGILFKGTIIGFK--SSPSVSSFSSRGPNLASPGIVKPDIIGPGVNILAAW 507

Query: 493 PFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPK 552
           P  ++N T T+LTFN+ISGTSMSCPHLSGI AL+KS HP+WSPAAIKSAIMT+A+    +
Sbjct: 508 PVSVENKTGTDLTFNIISGTSMSCPHLSGIVALLKSAHPDWSPAAIKSAIMTSADQSNLE 567

Query: 553 GKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YKDEQVSIIVH 612
           G+PILD+    A+ FA G+GHVNPSKA+DPGL+YDIQ +DYI YLCGL Y+++ + +IV 
Sbjct: 568 GQPILDERNLPADIFATGAGHVNPSKASDPGLIYDIQLEDYIQYLCGLGYREKDIGLIVQ 627

Query: 613 KTVICGLLPRIPEGDLNYPSFSVALG-ELQTFKRTVTNVGEANSVYTAIVEAPVGVSMKV 672
           +TV C     I E +LNYPSFS+ LG + Q + RTVTNVG+A+S YT  +    GV + V
Sbjct: 628 ETVKCE--SSISEAELNYPSFSIILGPKTQNYTRTVTNVGDASSTYTVNIAKTQGVDIVV 687

Query: 673 TPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPIVVK 694
            P  LVF+++ Q+ T++V+F++  S      F QG + W S ++VV SPI VK
Sbjct: 688 EPATLVFTKMYQQATYTVSFTQ--SGDFTDRFVQGAISWSSNEYVVRSPISVK 732

BLAST of Clc09G09150 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 1.1e-223
Identity = 410/714 (57.42%), Postives = 509/714 (71.29%), Query Frame = 0

Query: 12  STNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLT 71
           ST IVHV +P++       +DD+ ++Y S LP +T +T +  R++++YRNV++GF+ +LT
Sbjct: 43  STYIVHVRKPQV-----IQSDDLHTFYYSLLPESTKTTNQ--RIVFTYRNVVNGFAVKLT 102

Query: 72  KEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGI 131
            E+ KA+++ +  +SA PE I++LHTTHTP + GL Q LGLWK SN GKGVIIG+LDTGI
Sbjct: 103 PEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILDTGI 162

Query: 132 HPSHPSFSDEGMSPPPAKWKGRCEF--------------------NISSPSDEKGHGTHT 191
            P HPSFSDEGM  PPAKW G CEF                    N++ P D+ GHGTHT
Sbjct: 163 SPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLTLPFDDVGHGTHT 222

Query: 192 ASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDGVD 251
           AST+AG  V+GAN  GNA G A GMAP AH+A+YKVC +  C  S +LAG+D A+DDGVD
Sbjct: 223 ASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDDGVD 282

Query: 252 VLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGAST 311
           VLS+SLG  S PFFED IA+  F AIQKGIFVSCSA NSGP  S+LSNEAPWILTVGAS+
Sbjct: 283 VLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVGASS 342

Query: 312 LNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS-------------SLENMDV 371
           ++R I+A AKLGNG+EY G+S++QP D A + LPL YAG+             SL   DV
Sbjct: 343 IDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCAPESLNRSDV 402

Query: 372 KGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAGV 431
           +GKV++CE  G V +V KG  VK+AGGAAMIL+N   + F+  +  HVLPA H+SYEAG+
Sbjct: 403 EGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLPAVHISYEAGL 462

Query: 432 LIKSYINSSQKPTASISFEGTIIGDDEFSAPAMASFSSRGPCLPSPGILKPDIIGPGVNI 491
            +K YINS+  PTA+I FEGT+IG+    AP + SFSSRGP   SPGILKPDIIGPG+NI
Sbjct: 463 ALKEYINSTSTPTATILFEGTVIGN--LLAPQVTSFSSRGPSKASPGILKPDIIGPGLNI 522

Query: 492 LAAWPFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANI 551
           LAAWP  LDN+T     FN+ISGTSMSCPHLSGIAAL+K+ HP+WSPAAIKSAIMTTA+ 
Sbjct: 523 LAAWPVSLDNSTTP--PFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTASQ 582

Query: 552 KTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YKDEQVS 611
               G PILDQ L  A+ FA G+GHVNP KA DPGLVYDI+P+DYIPYLCGL Y D +V 
Sbjct: 583 VNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLNYTDREVG 642

Query: 612 IIVHKTVICGLLPRIPEGDLNYPSFSVALGE-LQTFKRTVTNVGEANSVYTAIVEAPVGV 671
           +I+ + V C  +  I E +LNYPSFS+ LG   Q + RTV NVG ANS YTA +  PVGV
Sbjct: 643 VILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTYTAEIGVPVGV 702

Query: 672 SMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPI 691
            M ++P +L F+ + QK+T+SV+F   S  +    F QG LKWVS K+ V SPI
Sbjct: 703 GMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYSVRSPI 745

BLAST of Clc09G09150 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 8.7e-221
Identity = 409/722 (56.65%), Postives = 517/722 (71.61%), Query Frame = 0

Query: 12  STNIVHVEQPKIRLVGE-SSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARL 71
           +T IVHV+  K+ + G   S +++ +W+ SFLP     T+   R+++SYRNV SGF+ RL
Sbjct: 39  TTYIVHVK--KLEIEGPLQSTEELHTWHHSFLP----ETSNKDRMVFSYRNVASGFAVRL 98

Query: 72  TKEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTG 131
           T E+  A+++K+  +S  PE  ++LHTTHTP + GL Q  GLW +SN+GKGVIIGV+DTG
Sbjct: 99  TPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTG 158

Query: 132 IHPSHPSFSDEGMSPPPAKWKGRCEFNISS--------------------PSDEKGHGTH 191
           I+P H SF+DEGM PPPAKWKG CEF   S                    P ++  HGTH
Sbjct: 159 IYPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGARNLVKSAIQEPPYEDFFHGTH 218

Query: 192 TASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVK---NCPSSDVLAGIDAAID 251
           TA+ +AG FVEGA+  GNARG AAGMAP AHLAIYKVCS K    CP S +LA +D AI+
Sbjct: 219 TAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMDIAIE 278

Query: 252 DGVDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTV 311
           DGVDVLS+SLG  S+PFFED IA+  FAA QKGIFVSCSA NSGP  S+LSNEAPWILTV
Sbjct: 279 DGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPWILTV 338

Query: 312 GASTLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS------------SLEN 371
           GAST++R+I A AKLGNG EY+GE+L+QP D +S  LPL YA +            SL N
Sbjct: 339 GASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPGSLRN 398

Query: 372 MDVKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYE 431
           ++VKGKV+VC+  GG+  +AKG  V +AGG+AMIL N +  GF+T + AHVLPA HVSY 
Sbjct: 399 INVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVHVSYA 458

Query: 432 AGVLIKSYINSSQKPTASISFEGTIIGDDEFSAPAMASFSSRGPCLPSPGILKPDIIGPG 491
           A + IK+YINS+  PTA++ F+GTIIGD    AP++A+FSSRGP   SPGILKPDIIGPG
Sbjct: 459 ASLAIKAYINSTYTPTATVLFQGTIIGDS--LAPSVAAFSSRGPSQQSPGILKPDIIGPG 518

Query: 492 VNILAAWPFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTT 551
           VNILAAW   +DN       F++ISGTSMSCPHLSGIAAL+KS HP+WSPAAIKSAIMTT
Sbjct: 519 VNILAAWAVSVDNKIP---AFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSAIMTT 578

Query: 552 ANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YKDE 611
           AN    +G PILDQ L+ A+ FA G+GHVNP +A DPGLVYDIQP+DY+PYLCGL Y D 
Sbjct: 579 ANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGYSDR 638

Query: 612 QVSIIVHKTVICGLLPRIPEGDLNYPSFSVALG-ELQTFKRTVTNVGEANSVYTAIVEAP 671
           +V+IIV ++V C  +  I + +LNYPSFS+ LG + Q + RT+TNVG ANS YT  ++ P
Sbjct: 639 EVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTVKIDVP 698

Query: 672 VGVSMKVTPRKLVFSRLNQKVTFSVTF-SRISSVQIVGEFGQGYLKWVSEKHVVGSPIVV 695
           + + + V+P ++ F+++NQKV + V F  +I   +    F QG + WVS+KHVV +PI V
Sbjct: 699 LAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVRTPISV 749

BLAST of Clc09G09150 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.6e-214
Identity = 405/722 (56.09%), Postives = 496/722 (68.70%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLTK 72
           T IVHV+  K   V    ++D+ SWY SFLP T        R+++SYR V SGF+ +LT 
Sbjct: 37  TYIVHVK--KSENVASHQSEDLHSWYHSFLPQT---FPHKERMVFSYRKVASGFAVKLTP 96

Query: 73  EQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGIH 132
           E+ K++++K   +SA PE  + LHTTHTP + GL Q  GLW + N+GKGVIIG++DTGI 
Sbjct: 97  EEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTGIF 156

Query: 133 PSHPSFSDEGMSPPPAKWKGRCEFN-------------------ISSPSDEK-GHGTHTA 192
           P HPSF+DEGM PPPAKWKG CEF                    I  P  E   HGTHTA
Sbjct: 157 PLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGARNLVKSAIQEPPFENFFHGTHTA 216

Query: 193 STSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVK-NCPSSDVLAGIDAAIDDGVD 252
           + +AG F+E A+  GNA+G AAGMAP AHLAIYKVC+ K  C  S +LA +D AI+DGVD
Sbjct: 217 AEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMDIAIEDGVD 276

Query: 253 VLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGAST 312
           VLS+SLG  S+PFFED IA+  FAA Q G+FVSCSA NSGP  STLSNEAPWILTVGAST
Sbjct: 277 VLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPWILTVGAST 336

Query: 313 LNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS----------------SLEN 372
           ++R+IVA AKLGNGEEY+GE+L+QP D +   LPL Y GS                SL+N
Sbjct: 337 IDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQSLCLPGSLKN 396

Query: 373 MDVKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYE 432
           +D+ GKV++C+  G V  + KG  V N+GG AMIL N +  GFST + AHVLPA  VSY 
Sbjct: 397 IDLSGKVVLCDV-GNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHVLPAVEVSYA 456

Query: 433 AGVLIKSYINSSQKPTASISFEGTIIGDDEFSAPAMASFSSRGPCLPSPGILKPDIIGPG 492
           AG+ IKSYI S+  PTA++ F+GTIIGD    AP++  FSSRGP   SPGILKPDIIGPG
Sbjct: 457 AGLTIKSYIKSTYNPTATLIFKGTIIGDS--LAPSVVYFSSRGPSQESPGILKPDIIGPG 516

Query: 493 VNILAAWPFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTT 552
           VNILAAW   +DN       F+++SGTSMSCPHLSGIAALIKS HP+WSPAAIKSAIMTT
Sbjct: 517 VNILAAWAVSVDNKIP---AFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTT 576

Query: 553 ANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YKDE 612
           AN     G PILDQ L  A+ FA G+GHVNP KA DPGLVYDI+P+DY+PYLCGL Y D+
Sbjct: 577 ANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYLCGLGYSDK 636

Query: 613 QVSIIVHKTVICGLLPRIPEGDLNYPSFSVALG-ELQTFKRTVTNVGEANSVYTAIVEAP 672
           ++ +IV   V C  +  IPE  LNYPSFS+ LG + Q + RT+TNVG ANS Y   +E P
Sbjct: 637 EIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANSTYKVELEVP 696

Query: 673 VGVSMKVTPRKLVFSRLNQKVTFSVTF-SRISSVQIVGEFGQGYLKWVSEKHVVGSPIVV 695
           + + M V P ++ F+ +N+KV+FSV F  +I   +    FGQG L WVS++H V  PI V
Sbjct: 697 LALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRHAVRIPISV 747

BLAST of Clc09G09150 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 582/719 (80.95%), Postives = 638/719 (88.73%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLTK 72
           T IVHVEQPKIRLVGESSND IESWYMSF+P +T +T E P+LLYSYRNVMSGFSARLT 
Sbjct: 36  TYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTI 95

Query: 73  EQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGIH 132
           EQVKAM+KKDGFISAMPETIM+LHTTHTP+Y GLNQ  G+WKNSN GKGVI+GVLDTGIH
Sbjct: 96  EQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIH 155

Query: 133 PSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGTH 192
           P+HPSF+DEGMSPPPAKWKG+CEFN S                      SP+DEKGHGTH
Sbjct: 156 PNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTH 215

Query: 193 TASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDGV 252
           TAST+AGAFVE A ALGNA+GKAAG+APLAHLAIYKVCS K CPSSDV AGIDAAIDDGV
Sbjct: 216 TASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGV 275

Query: 253 DVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGAS 312
           DVLSISLG+ SVPFF+D+IAVATF AIQKGIFVS SAGNSGP +STLSN+APWILTVGAS
Sbjct: 276 DVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGAS 335

Query: 313 TLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS------------SLENMDV 372
           T+NRRIVAVAKLGNGE+Y+GESLYQP+D  S FLPL YAG+            SLENMDV
Sbjct: 336 TINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDV 395

Query: 373 KGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAGV 432
           KGKV+VCE KGGVG+VAKG+VVKNAGGAAMILINQ++DGFST S+AHVLPA HVSY+AGV
Sbjct: 396 KGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGV 455

Query: 433 LIKSYINSSQKPTASISFEGTIIGD-DEFSAPAMASFSSRGPCLPSPGILKPDIIGPGVN 492
           LIKSYINSSQ PTASISF+GT+IGD D+FSAP+MASFSSRGPCLPSPGILKPDI GPGVN
Sbjct: 456 LIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVN 515

Query: 493 ILAAWPFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN 552
           ILAAWPFPLDN+TNT  TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN
Sbjct: 516 ILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN 575

Query: 553 IKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEQVS 612
           IKTP+G+PI+DQDL+ ANFFAMG+GHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE+VS
Sbjct: 576 IKTPQGEPIVDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVS 635

Query: 613 IIVHKTVICGLLPRIPEGDLNYPSFSVALGELQTFKRTVTNVGEANSVYTAIVEAPVGVS 672
           IIVH+TVICGL+P I EGDLNYPSFSVALG LQTFKRTVTNVGEANSVYTAIVEAP+GVS
Sbjct: 636 IIVHRTVICGLVPSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVS 695

Query: 673 MKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWV--SEKHVVGSPIVVKF 695
           M V PRKL FSR+NQ +TF+VTF+RI  V+IVGEFG+GYLKWV  S+K+VV SP+ VKF
Sbjct: 696 MTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 754

BLAST of Clc09G09150 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 581/719 (80.81%), Postives = 636/719 (88.46%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLTK 72
           T IVHVEQPKIRLVGESSND IESWYMSF+P +T +T E P+LLYSYRNVMSGFSARLT 
Sbjct: 45  TYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTI 104

Query: 73  EQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGIH 132
           EQVKAM+KKDGFISAMPETIM+LHTTHTP+Y GLNQ  G+WKNSN GKGVI+GVLDTGIH
Sbjct: 105 EQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIH 164

Query: 133 PSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGTH 192
           P+HPSF+DEGMSPPPAKWKG+CEFN S                      SP+DEKGHGTH
Sbjct: 165 PNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTH 224

Query: 193 TASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDGV 252
           TAST+AGAFVE A ALGNA+GKAAG+APLAHLAIYKVCS K CPSSDV AGIDAAIDDGV
Sbjct: 225 TASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGV 284

Query: 253 DVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGAS 312
           DVLSISLG+ SVPFF+D+IAVATF AIQKGIFVS SAGNSGP +STLSN+APWILTVGAS
Sbjct: 285 DVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGAS 344

Query: 313 TLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS------------SLENMDV 372
           T+NRRIVAVAKLGNGE+Y+GESLYQP+D  S FLPL YAG+            SLENMDV
Sbjct: 345 TINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDV 404

Query: 373 KGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAGV 432
           KGKV+VCE KGGVG+VAKG+VVKNAGGAAMILINQ++DGFST S+AHVLPA HVSY+AGV
Sbjct: 405 KGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGV 464

Query: 433 LIKSYINSSQKPTASISFEGTIIGD-DEFSAPAMASFSSRGPCLPSPGILKPDIIGPGVN 492
           LIKSYINSSQ PTASISF+GT+IGD D+FSAP+MASFSSRGPCLPSPGILKPDI GPGVN
Sbjct: 465 LIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVN 524

Query: 493 ILAAWPFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN 552
           ILAAWPFPLDN+TNT  TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN
Sbjct: 525 ILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTAN 584

Query: 553 IKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEQVS 612
           IKTP+G+PI DQDL+ ANFFAMG+GHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE+VS
Sbjct: 585 IKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVS 644

Query: 613 IIVHKTVICGLLPRIPEGDLNYPSFSVALGELQTFKRTVTNVGEANSVYTAIVEAPVGVS 672
           IIVH+TVICGL+  I EGDLNYPSFSVALG LQTFKRTVTNVGEANSVYTAIVEAP+GVS
Sbjct: 645 IIVHRTVICGLVLSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVS 704

Query: 673 MKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWV--SEKHVVGSPIVVKF 695
           M V PRKL FSR+NQ +TF+VTF+RI  V+IVGEFG+GYLKWV  S+K+VV SP+ VKF
Sbjct: 705 MTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 763

BLAST of Clc09G09150 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 8.8e-285
Identity = 507/720 (70.42%), Postives = 584/720 (81.11%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPAT-TASTTEPPRLLYSYRNVMSGFSARLT 72
           T IVHV+QP + ++G++   D+++WY SFLP T  AS+ E PRLLYSYR+VMSGFSARLT
Sbjct: 35  TYIVHVKQPVLEILGDTI--DLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLT 94

Query: 73  KEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGI 132
           KEQVKAM++KDGFISAMPET +NLHTTHTP+Y GLN+  GLWKNSN GKGVIIGVLDTGI
Sbjct: 95  KEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGI 154

Query: 133 HPSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGT 192
           HP+HPSF+DEGMS PPAKWKGRCEF  S                      SP+DE GHGT
Sbjct: 155 HPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHGT 214

Query: 193 HTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDG 252
           HTAST+AG FV+GA ALGNARGKA GMAPLAH+A+YKVCS   C SSD+LA +DAAIDDG
Sbjct: 215 HTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDG 274

Query: 253 VDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGA 312
           VDVLS+SLGA S PFF D IA+  FAAI+KGIFVSCSAGNSGP  +TL+NEAPWILTVGA
Sbjct: 275 VDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGA 334

Query: 313 STLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAG------------SSLENMD 372
           ST++R+IVA+AKLG+GE Y GESL+QP +  S FLPL YAG             SLE ++
Sbjct: 335 STIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLN 394

Query: 373 VKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAG 432
           V GKV+VCE  GG+ +VAKG+VVKN GGAAMILINQ+ +GFST ++AHVLP  H+SYE G
Sbjct: 395 VTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDG 454

Query: 433 VLIKSYINSSQKPTASISFEGTIIGDDEFS-APAMASFSSRGPCLPSPGILKPDIIGPGV 492
           + IK+YINSS  P ASISF+GT++G+   + +PAMASFSSRGPC  SPGILKPDI GPGV
Sbjct: 455 LKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGV 514

Query: 493 NILAAWPFPLDNNTNTNL--TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 552
           NILAAWPFPL+NNTNTN   TFNVISGTSMSCPHLSGIAALIKS HPNWSPAAIKSAIMT
Sbjct: 515 NILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMT 574

Query: 553 TANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 612
           +A+++ P+GKPI+DQDL+ ANFFAMGSGHVNPSKAA+PGLVYDIQPDDYIPYLC LY D 
Sbjct: 575 SADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDA 634

Query: 613 QVSIIVHKTVICGLLPRIPEGDLNYPSFSVALGE-LQTFKRTVTNVGEANSVYTAIVEAP 672
           QVSIIV K V C  +PRI EGDLNYPSF+V+LG   QTF RTVTNVG+ANSVY  IVEAP
Sbjct: 635 QVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVEAP 694

Query: 673 VGVSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPIVVK 694
            GVS+KVTP  L FS+LN+KVT+SVTFSRI  V+   EF +GYL WVS+K +V SPI VK
Sbjct: 695 AGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 752

BLAST of Clc09G09150 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 5.7e-284
Identity = 506/720 (70.28%), Postives = 582/720 (80.83%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPAT-TASTTEPPRLLYSYRNVMSGFSARLT 72
           T IVHV+QP + ++G++   D+++WY SFLP T  AS+ E PRLLYSYR+VMSGFSARLT
Sbjct: 15  TYIVHVKQPVLEILGDTI--DLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLT 74

Query: 73  KEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGI 132
           KEQVKAM++KDGFISAMPET +NLHTTHTP+Y GLN   GLWKNSN GKGVIIGVLDTGI
Sbjct: 75  KEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGI 134

Query: 133 HPSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGT 192
           HP+HPSF+DEGMS PPAKWKGRCEF  S                      SP+DE GHGT
Sbjct: 135 HPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHGT 194

Query: 193 HTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDG 252
           HTAST+AG FV+GA ALGNARGKA GMAPLAH+A+YKVCS   C SSD+LA +DAAIDDG
Sbjct: 195 HTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDG 254

Query: 253 VDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGA 312
           VDVLS+SLGA S PFF D IA+  FAAI+KGIFVSCSAGNSGP  +TL+NEAPWILTVGA
Sbjct: 255 VDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGA 314

Query: 313 STLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAG------------SSLENMD 372
           ST++R+IVA+AKLG+GE Y GESL+QP +  S FLPL YAG             SLE ++
Sbjct: 315 STIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLN 374

Query: 373 VKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAG 432
           V GKV+VCE  GG+ +VAKG+VVKN GGAAMILINQ+ +GFST ++AHVLP  H+SYE G
Sbjct: 375 VTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDG 434

Query: 433 VLIKSYINSSQKPTASISFEGTIIGDDEFS-APAMASFSSRGPCLPSPGILKPDIIGPGV 492
           + IK+YINSS  P ASISF+GT++G+   + +PAMASFSSRGPC  SPGILKPDI GPGV
Sbjct: 435 LKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGV 494

Query: 493 NILAAWPFPLDNNTNTNL--TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 552
           NILAAWPFPL+NNTNTN   TFNVISGTSMSCPHLSGIAALIKS HPNWSPAAIKSAIMT
Sbjct: 495 NILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMT 554

Query: 553 TANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 612
           +A+++ P+GKPI+DQDL+ ANFFAMGSGHVNPSKAA+PGLVYDIQPDDYIPYLC LY D 
Sbjct: 555 SADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDA 614

Query: 613 QVSIIVHKTVICGLLPRIPEGDLNYPSFSVAL-GELQTFKRTVTNVGEANSVYTAIVEAP 672
           QVSIIV K V C  +PRI EGDLNYPSF+V+L    QTF RTVTNVG+ANSVY  IVEAP
Sbjct: 615 QVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVEAP 674

Query: 673 VGVSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPIVVK 694
            GVS+KVTP  L FS+LN+KVT+SVTFSRI  V+   EF +GYL WVS+K +V SPI VK
Sbjct: 675 AGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 732

BLAST of Clc09G09150 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 5.7e-284
Identity = 500/720 (69.44%), Postives = 585/720 (81.25%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPAT-TASTTEPPRLLYSYRNVMSGFSARLT 72
           T IVHV+QP++ ++G++   D+++WY SFLP T  AS+ E  RLLYSYR+V+SGFSARLT
Sbjct: 15  TYIVHVKQPEVEILGDTI--DLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSARLT 74

Query: 73  KEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVLDTGI 132
           KEQVK M++KDGFISAMPET +NLHTTHTP+Y GLNQ  GLWKNSN GKGVIIGVLDTGI
Sbjct: 75  KEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGI 134

Query: 133 HPSHPSFSDEGMSPPPAKWKGRCEFNIS----------------------SPSDEKGHGT 192
           HP+HPSF+DEGM  PPAKWKGRCEF  S                      SP+DE GHGT
Sbjct: 135 HPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKSPNDENGHGT 194

Query: 193 HTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDG 252
           HTAST+AG FV+GA ALGNARGKA GMAPLAH+A+YKVCS K C SSD+LA +DAAIDDG
Sbjct: 195 HTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDAAIDDG 254

Query: 253 VDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGA 312
           VDVLS+SLGA S PFF+D IAV  FAAI+KGIFVSCSAGNSGP  +TL+NEAPWILTVGA
Sbjct: 255 VDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGA 314

Query: 313 STLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAG------------SSLENMD 372
           ST++R+IVA+AKL +G+ + GESL+QP D +S FLPL YAG             SLE ++
Sbjct: 315 STIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLN 374

Query: 373 VKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAG 432
           V GK++VCE  GG+G++AKG+VVKN GGAAMIL+NQ+ DGFST ++AHVLP  H+SYE G
Sbjct: 375 VTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHLSYEDG 434

Query: 433 VLIKSYINSSQKPTASISFEGTIIGDDEFS-APAMASFSSRGPCLPSPGILKPDIIGPGV 492
           + IK YINSS  P ASISFEGT++G+   + +PAMASFSSRGPC  SPGILKPDI GPGV
Sbjct: 435 LKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGV 494

Query: 493 NILAAWPFPLDNNTNTNL--TFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 552
           NILAAWPFPL+NNTNTN   TFNVISGTSMSCPHLSGIAALIKS HPNWSPAAIKSAIMT
Sbjct: 495 NILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPAAIKSAIMT 554

Query: 553 TANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 612
           +A+++ P+GKPI+DQDL+ ANFFAMGSGHVNPSKAA+PGLVYDIQPDDY+PYLC LY D 
Sbjct: 555 SADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDA 614

Query: 613 QVSIIVHKTVICGLLPRIPEGDLNYPSFSVALG-ELQTFKRTVTNVGEANSVYTAIVEAP 672
           QVSIIV + V C  + RI EGDLNYPSF+V+LG + Q F RTVTNVG+ANSVY AIV+AP
Sbjct: 615 QVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVKAP 674

Query: 673 VGVSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQGYLKWVSEKHVVGSPIVVK 694
            GVS++VTPR L FS+LN+K+T+SVTFSRI  V+   EF +GYL WVS KH+V SPI VK
Sbjct: 675 AGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISVK 732

BLAST of Clc09G09150 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 538.5 bits (1386), Expect = 7.9e-153
Identity = 319/739 (43.17%), Postives = 439/739 (59.40%), Query Frame = 0

Query: 13  TNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNVMSGFSARLTK 72
           T IVH+ + ++     SS D   +WY S L     S ++   LLY+Y N + GFS RLT+
Sbjct: 31  TYIVHMAKSQM----PSSFDLHSNWYDSSL----RSISDSAELLYTYENAIHGFSTRLTQ 90

Query: 73  EQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQ-PLGLWKNSNIGKGVIIGVLDTGI 132
           E+  ++  + G IS +PE    LHTT TP + GL++    L+  +     V++GVLDTG+
Sbjct: 91  EEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGV 150

Query: 133 HPSHPSFSDEGMSPPPAKWKGRCE----FNIS---------------------------- 192
            P   S+SDEG  P P+ WKG CE    F  S                            
Sbjct: 151 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKE 210

Query: 193 --SPSDEKGHGTHTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSD 252
             SP D+ GHGTHT+ST+AG+ VEGA+ LG A G A GMAP A +A+YKVC +  C SSD
Sbjct: 211 SRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSD 270

Query: 253 VLAGIDAAIDDGVDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTL 312
           +LA ID AI D V+VLS+SLG     ++ D +A+  FAA+++GI VSCSAGN+GP SS+L
Sbjct: 271 ILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSL 330

Query: 313 SNEAPWILTVGASTLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAYAGS------ 372
           SN APWI TVGA TL+R   A+A LGNG+ + G SL++   +    LP  YAG+      
Sbjct: 331 SNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATN 390

Query: 373 -------SLENMDVKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQA 432
                  +L    VKGK+++C+ +G   +V KG VVK AGG  MIL N   +G    + A
Sbjct: 391 GNLCMTGTLIPEKVKGKIVMCD-RGINARVQKGDVVKAAGGVGMILANTAANGEELVADA 450

Query: 433 HVLPAAHVSYEAGVLIKSYINSSQKPTASISFEGTIIGDDEFSAPAMASFSSRGPCLPSP 492
           H+LPA  V  +AG +I+ Y+ +   PTASIS  GT++G     +P +A+FSSRGP   +P
Sbjct: 451 HLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK--PSPVVAAFSSRGPNSITP 510

Query: 493 GILKPDIIGPGVNILAAW-----PFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSV 552
            ILKPD+I PGVNILAAW     P  L +++   + FN+ISGTSMSCPH+SG+AAL+KSV
Sbjct: 511 NILKPDLIAPGVNILAAWTGAAGPTGLASDSR-RVEFNIISGTSMSCPHVSGLAALLKSV 570

Query: 553 HPNWSPAAIKSAIMTTANIKTPKGKPILD-QDLRAANFFAMGSGHVNPSKAADPGLVYDI 612
           HP WSPAAI+SA+MTTA      GKP+LD    + +  F  G+GHV+P+ A +PGL+YD+
Sbjct: 571 HPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDL 630

Query: 613 QPDDYIPYLCGL-YKDEQVSIIVHKTVICGLLPRIPEGDLNYPSFSVALGELQTFK--RT 672
             +DY+ +LC L Y   Q+  +  +   C         DLNYPSF+V +  +  +K  RT
Sbjct: 631 TTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRT 690

Query: 673 VTNVGEANSVYTAIVEAPVGVSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGEFGQG 695
           VT+VG A +    +     GV + V P  L F   N+K +++VTF+ + S +  G    G
Sbjct: 691 VTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSKPSGSNSFG 750

BLAST of Clc09G09150 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 536.6 bits (1381), Expect = 3.0e-152
Identity = 317/730 (43.42%), Postives = 431/730 (59.04%), Query Frame = 0

Query: 37  WYMSFLPATTASTTE-----PPRLLYSYRNVMSGFSARLTKEQVKAMKKKDGFISAMPET 96
           W++SFL        E       RLLYSY + + GF+A+LT+ + + ++     ++  P+ 
Sbjct: 48  WHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDH 107

Query: 97  IMNLHTTHTPDYFGLN--QPLGLWKNSNIGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAK 156
           ++ + TT++  + GL+     G+W  S  G+G IIGVLDTG+ P  PSF D GM   P K
Sbjct: 108 VLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRK 167

Query: 157 WKGRCE----FNIS---------------------------------SPSDEKGHGTHTA 216
           WKG C+    F+ S                                 S  D  GHGTHTA
Sbjct: 168 WKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTA 227

Query: 217 STSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKNCPSSDVLAGIDAAIDDGVDV 276
           ST  G+ V  AN LGN  G A GMAP AH+A+YKVC    C SSD+LA ID AI D VDV
Sbjct: 228 STVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDV 287

Query: 277 LSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGPFSSTLSNEAPWILTVGASTL 336
           LS+SLG   +P ++D IA+ TF A+++GI V C+AGN+GP  S+++N APW+ T+GA TL
Sbjct: 288 LSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTL 347

Query: 337 NRRIVAVAKLGNGEEYDGESLYQPSDV--ASTFLPLAYA-----GS------SLENMDVK 396
           +RR  AV +L NG+   GESLY    +  A   + + Y      GS      SL   +++
Sbjct: 348 DRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIR 407

Query: 397 GKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQSQAHVLPAAHVSYEAGVL 456
           GK+++C+ +G  G+  KG  VK AGG AMIL N + +        H+LPA  + Y   VL
Sbjct: 408 GKMVICD-RGVNGRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVL 467

Query: 457 IKSYINSSQKPTASISFEGTIIGDDEFSAPAMASFSSRGPCLPSPGILKPDIIGPGVNIL 516
           +K+Y+N++ KP A I F GT+IG     AP +A FS+RGP L +P ILKPD+I PGVNI+
Sbjct: 468 LKAYVNATVKPKARIIFGGTVIGRSR--APEVAQFSARGPSLANPSILKPDMIAPGVNII 527

Query: 517 AAWP-------FPLDNNTNTNLTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAI 576
           AAWP        P D+     + F V+SGTSMSCPH+SGI ALI+S +PNWSPAAIKSA+
Sbjct: 528 AAWPQNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSAL 587

Query: 577 MTTANIKTPKGKPILDQDLRAANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-Y 636
           MTTA++   +GK I D + + A  FA+G+GHVNP KA +PGLVY+IQP DYI YLC L +
Sbjct: 588 MTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGF 647

Query: 637 KDEQVSIIVHKTVIC-GLLPRIPEGDLNYPSFSVALGELQT---FKRTVTNVGEANSVYT 693
               +  I HK V C G+L + P   LNYPS +V     +T     R VTNVG  NS+Y+
Sbjct: 648 TRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYS 707

BLAST of Clc09G09150 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 536.2 bits (1380), Expect = 3.9e-152
Identity = 323/759 (42.56%), Postives = 440/759 (57.97%), Query Frame = 0

Query: 3   PQKLSFQLFSTNIVHVEQPKIRLVGESSNDDIESWYMSFLPATTASTTEPPRLLYSYRNV 62
           P   S     + IVHV++     +  S N    +W++S L  +  S+ +P  LLYSY   
Sbjct: 22  PSSSSSDGLESYIVHVQRSHKPSLFSSHN----NWHVSLL-RSLPSSPQPATLLYSYSRA 81

Query: 63  MSGFSARLTKEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGV 122
           + GFSARL+  Q  A+++    IS +P+    +HTTHTP + G +Q  GLW NSN G+ V
Sbjct: 82  VHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDV 141

Query: 123 IIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCEF-------------------------- 182
           I+GVLDTGI P HPSFSD G+ P P+ WKG CE                           
Sbjct: 142 IVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQ 201

Query: 183 ----------NISSPSDEKGHGTHTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYK 242
                        SP D +GHGTHTAST+AG+ V  A+    ARG A GMA  A +A YK
Sbjct: 202 RNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYK 261

Query: 243 VCSVKNCPSSDVLAGIDAAIDDGVDVLSISLGAD-SVP-FFEDHIAVATFAAIQKGIFVS 302
           +C    C  SD+LA +D A+ DGV V+S+S+GA  S P +  D IA+  F A + GI VS
Sbjct: 262 ICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVS 321

Query: 303 CSAGNSGPFSSTLSNEAPWILTVGASTLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFL 362
           CSAGNSGP   T +N APWILTVGAST++R   A A  G+G+ + G SLY    +  + L
Sbjct: 322 CSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQL 381

Query: 363 PLAYAG---------SSLENMDVKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQ 422
            L Y+G           L +  V+GK+++C+ +GG  +V KG  VK AGGA MIL N  +
Sbjct: 382 SLVYSGDCGSRLCYPGKLNSSLVEGKIVLCD-RGGNARVEKGSAVKLAGGAGMILANTAE 441

Query: 423 DGFSTQSQAHVLPAAHVSYEAGVLIKSYINSSQKPTASISFEGTIIGDDEFSAPAMASFS 482
            G    + +H++PA  V  +AG  I+ YI +S  PTA ISF GT+IG     +P +A+FS
Sbjct: 442 SGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP-PSPRVAAFS 501

Query: 483 SRGPCLPSPGILKPDIIGPGVNILAAW-----PFPLDNNTNTNLTFNVISGTSMSCPHLS 542
           SRGP   +P ILKPD+I PGVNILA W     P  LD +    + FN+ISGTSMSCPH+S
Sbjct: 502 SRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPHVS 561

Query: 543 GIAALIKSVHPNWSPAAIKSAIMTTANIKTPKGKPILDQDL-RAANFFAMGSGHVNPSKA 602
           G+AAL++  HP+WSPAAIKSA++TTA      G+PI D    +++N F  G+GHV+P+KA
Sbjct: 562 GLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKA 621

Query: 603 ADPGLVYDIQPDDYIPYLCGL-YKDEQVSIIVHKTVI---CGLLPRIPEGDLNYPSFSV- 662
            +PGLVYDI+  +Y+ +LC + Y+   + + +    +   C        GDLNYPSFSV 
Sbjct: 622 LNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVV 681

Query: 663 --ALGELQTFKRTVTNVG-EANSVYTAIVEAPVGVSMKVTPRKLVFSRLNQKVTFSVTFS 695
             + GE+  +KR V NVG   ++VY   V++P  V + V+P KL FS+    + + VTF 
Sbjct: 682 FASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTF- 741

BLAST of Clc09G09150 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 521.2 bits (1341), Expect = 1.3e-147
Identity = 305/702 (43.45%), Postives = 419/702 (59.69%), Query Frame = 0

Query: 53  PRLLYSYRNVMSGFSARLTKEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGL 112
           P + Y Y N MSGFSA LT +Q+  +K   GFISA P+ +++LHTT++ ++ GL   +GL
Sbjct: 77  PEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGL 136

Query: 113 WKNSNIGKGVIIGVLDTGIHPSHPSFSDEGMSPPPAKWKGRCE----------------- 172
           W  +++   VIIG++DTGI P H SF D  M+P P++W+G C+                 
Sbjct: 137 WNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGA 196

Query: 173 -----------------FNISSPSDEKGHGTHTASTSAGAFVEGANALGNARGKAAGMAP 232
                             +  S  D +GHGTHTAST+AG  V  AN  G A+G A+GM  
Sbjct: 197 SAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRF 256

Query: 233 LAHLAIYKVCSVKNCPSSDVLAGIDAAIDDGVDVLSISLGADSVPFFEDHIAVATFAAIQ 292
            + +A YK C    C S+DV+A ID AI DGVDV+S+SLG  S PF+ D IA+A F A+Q
Sbjct: 257 TSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQ 316

Query: 293 KGIFVSCSAGNSGPFSSTLSNEAPWILTVGASTLNRRIVAVAKLGNGEEYDGESLYQPSD 352
           K IFVSCSAGNSGP +ST+SN APW++TV AS  +R   A+ ++GN +   G SLY+   
Sbjct: 317 KNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGKS 376

Query: 353 VASTFLPLAY---AG----------SSLENMDVKGKVIVCEAKGGVGKVAKGIVVKNAGG 412
           + +  LPLA+   AG           SL+   V+GK+++C  +G  G+ AKG  VK +GG
Sbjct: 377 LKN--LPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVIC-LRGASGRTAKGEEVKRSGG 436

Query: 413 AAMILINQQQDGFSTQSQAHVLPAAHVSYEAGVLIKSYINSSQKPTASISFEGTIIGDDE 472
           AAM+L++ + +G    +  HVLPA  + +  G  + +Y+  +   TAS+ F GT  G   
Sbjct: 437 AAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATASVRFRGTAYG--- 496

Query: 473 FSAPAMASFSSRGPCLPSPGILKPDIIGPGVNILAAW-PF---PLDNNTNTNLTFNVISG 532
            +AP +A+FSSRGP +  P I KPDI  PG+NILA W PF    L  +    + FN+ISG
Sbjct: 497 ATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISG 556

Query: 533 TSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPKGKPILDQDL----RAANFF 592
           TSM+CPH+SGIAALIKSVH +WSPA IKSAIMTTA I   + +PI D+       AA  F
Sbjct: 557 TSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAF 616

Query: 593 AMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YKDEQVSIIVHKTVICGL-LPRIPEG 652
           A G+G+V+P++A DPGLVYD    DY+ YLC L Y  E++ +       C      +  G
Sbjct: 617 AFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPG 676

Query: 653 DLNYPSFSVAL---GELQT--FKRTVTNVGEANSVYTAIVEAPVGVSMKVTPRKLVFSRL 693
           DLNYPSF+V L     L+T  +KRTVTNVG     Y   VE P GV ++V P+ L F + 
Sbjct: 677 DLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKA 736

BLAST of Clc09G09150 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 508.4 bits (1308), Expect = 8.8e-144
Identity = 305/744 (40.99%), Postives = 419/744 (56.32%), Query Frame = 0

Query: 15  IVHVEQPKIRLVGESSNDDIES------WYMSFLPATTASTTEPPRLLYSYRNVMSGFSA 74
           ++H    K  ++  + +D  ES      WY S L + ++       LLY+Y     GFSA
Sbjct: 21  LLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSESS-------LLYTYTTSFHGFSA 80

Query: 75  RL-TKEQVKAMKKKDGFISAMPETIMNLHTTHTPDYFGLNQPLGLWKNSNIGKGVIIGVL 134
            L + E    +   +  +    + +  LHTT TP++ GLN   G+    +   GVIIGVL
Sbjct: 81  YLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVL 140

Query: 135 DTGIHPSHPSFSDEGMSPPPAKWKGRCE-----------------------FNIS----- 194
           DTG+ P   SF D  M   P+KWKG CE                       F ++     
Sbjct: 141 DTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGF 200

Query: 195 -------SPSDEKGHGTHTASTSAGAFVEGANALGNARGKAAGMAPLAHLAIYKVCSVKN 254
                  SP D  GHGTHT++T+AG+ V  A+ LG A G A GMA  A +A YKVC    
Sbjct: 201 SSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTG 260

Query: 255 CPSSDVLAGIDAAIDDGVDVLSISLGADSVPFFEDHIAVATFAAIQKGIFVSCSAGNSGP 314
           C  SD+LA +D AI DGVDVLS+SLG  S P++ D IA+  F+A+++G+FVSCSAGNSGP
Sbjct: 261 CFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGP 320

Query: 315 FSSTLSNEAPWILTVGASTLNRRIVAVAKLGNGEEYDGESLYQPSDVASTFLPLAY---- 374
             ++++N APW++TVGA TL+R   A A LGNG+   G SLY    + +  L L Y    
Sbjct: 321 TRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGN 380

Query: 375 -------AGSSLENMDVKGKVIVCEAKGGVGKVAKGIVVKNAGGAAMILINQQQDGFSTQ 434
                     SL++  V+GK++VC+ +G   +V KG VV++AGG  MI+ N    G    
Sbjct: 381 SSSSNLCLPGSLDSSIVRGKIVVCD-RGVNARVEKGAVVRDAGGLGMIMANTAASGEELV 440

Query: 435 SQAHVLPAAHVSYEAGVLIKSYINSSQKPTASISFEGTIIGDDEFSAPAMASFSSRGPCL 494
           + +H+LPA  V  + G L++ Y+ S  KPTA + F+GT++  D   +P +A+FSSRGP  
Sbjct: 441 ADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL--DVKPSPVVAAFSSRGPNT 500

Query: 495 PSPGILKPDIIGPGVNILAAW-----PFPLDNNTNTNLTFNVISGTSMSCPHLSGIAALI 554
            +P ILKPD+IGPGVNILA W     P  LD ++     FN++SGTSMSCPH+SG+A L+
Sbjct: 501 VTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR-RTQFNIMSGTSMSCPHISGLAGLL 560

Query: 555 KSVHPNWSPAAIKSAIMTTANIKTPKGKPILD-QDLRAANFFAMGSGHVNPSKAADPGLV 614
           K+ HP WSP+AIKSA+MTTA +      P+ D  D   +N +A GSGHV+P KA  PGLV
Sbjct: 561 KAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLV 620

Query: 615 YDIQPDDYIPYLCGL--YKDEQVSIIVHKTVICGLLPRIPEGDLNYPSFSVALG--ELQT 674
           YDI  ++YI +LC L    D  V+I+   +V C      P G LNYPSFSV  G   +  
Sbjct: 621 YDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGGKRVVR 680

Query: 675 FKRTVTNVGEANSVYTAIVEAPVGVSMKVTPRKLVFSRLNQKVTFSVTFSRISSVQIVGE 696
           + R VTNVG A+SVY   V     V + V P KL F  + +K  ++VTF     V +  +
Sbjct: 681 YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNK 740