Clc09G09140 (gene) Watermelon (cordophanus) v2

Overview
NameClc09G09140
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionPectinesterase
LocationClcChr09: 7870088 .. 7875758 (+)
RNA-Seq ExpressionClc09G09140
SyntenyClc09G09140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTATAGGGCTACCAAAGGAACTTCCCTTCTCTCTTTATAACCCACCAACCCTCTCTGCCTCTCTTCACAACTCTTCTCATTTCCGAGCTATGGAAAACACAAAACTTTCATTACCTCTCTTTGCTGCTTTACTCCTCACAAATGCTATTGTTGTTTTAGCCAATTCCAATGAGAATAGTGACATGAATAATAACTCGAGTGTCATAGAAAACTCATGTGCCATTACTCTTTATCCTCAACTATGTCAATCAACAATATACTCCATCGTCGGAACATCCAACTTGTTGAGTTTGAAAGATATTGTTGAAGTGTCATTGAGTGTTGCTGTTGATGCAGCTAAGCATAACTACAAAAGCATCAACAAACTTTGGATGTCACTTAATGATGTTAGTAAACGAGACAAGATTGCGCTTCGTGATTGTGTGGAAACCACAGACAGGGCGATTTATGAACTTCACAAGGCCGTAGAGGATCTTCGTGAGTATCCCAACAAGAAATCTCTTACCTTGTACGCTGATGACCTCAAAACCTTTCTCAGTTCTGCCATTACTAATCAGGTATATATATATATATTTATAATTAAGTGTTTGTAATTTTTGGAACATGTGCTTGTAGATCACAAAATGACTCTTTTTTTCCCGAAATTTAGAGAGGTTTACGTATTAGAATGCTTTCTAACATTTTTGAATCAAAAGTGGAGACTGTGGACTGGAGTTATATATATATATATATATTAAAAATTTGAAGAAATATTTAACTATATTCTGAAAATTTTCTCTATTAATAATGTGGTTGCAAAATATTTTGTTTTTTTATTTTTAATTTTCTAAATTTATGTTTGATATTTCTTAATTTTCTTCCTATGGTTTTCCCATTGTATAAAGAAGCATTTTTTAATTCTTAGTCAAAATGTATATACACAAAAATAAGTCTTTTAAAAACTATGGTCCGTTTTGTAACTATATTTCTTTTTTAGCTTCTGTTTTTTAAATTTAAGTCTATATAAATACTATTTTCACCTTCAAATATCTCTCTTTGTTGCCTAGTTTTTACCAAAGGTTTAAAAACCAAGCCAAATTTTGATAACTAAAAAAAAGTAGCTGTTCAATTATCTTTTTTTTCTTTTTGAAATTTGACTAAGAATCCAACTATTTGTAAGAAGTGCGGAGGAAATAGGCTTAATTTCTAAAAACAAAAAACAAAGAACAGAATGGTTACCAAAACATAACTTGATTTTTGAAACTATGGATATAAAATAGAGTTTTGAAGCTTAGTTTTTAAAAACTTTAACCTAGAAACTAAGGCTTCCTTTTGTATCTATTTAATTTTTTAGATTTTGGTTTTTAAATTTAAGGCTATTTCTTCTCAATTTCTTACAATATTTTCATTTTTATTAAGTAGGAGTTGAATTCTTAGCAAAATTTTAAAAACAAAAGCAAGTTTTTAAAACTACATTTTTTTAGTTTTCAAAACTTTGCTTAATTTTTTTAAATAGTGGTAAATAGCATATAACAAAAAAAAAAAAAAAATGTAGGCGTTCGAAGTGCTGAGTTGGTTATTATAATTAGATTATTATAGTTTATGAGTTATAATAGTTTGTATCCAGTTATAATAGTATGTGTTTGAACTGTAGACTATATAATTTGGGTAAGAAACAATAAACACTATAACATAGAGGGAGAGAGAAGATAGATAGGAAATAGTAAATCTGGGGGATATCGATTTCAAATAATATTACAGTAAATAATTGTATGTTATAAATAATTGTAAACATCACTATTATACTTGAAATCCCAAACATGAAGTGAGCTATAATAATTCACTCATCTAACTTCAAATTAGGGAGCCAAACACCCTCTTAAAGGTAAAAAGAGTATTTATAGACTTAAAAGCACTTATTTATTTATTTACGCTTACTTTGAATATAGTAATATATTTTTTTTTCAGGAGACTTGTTTGGAGGGATTGTCACATGAGAAAAGGGTGTTGAAGCTGATTGAAAGCACACATATTCACGTCACAAAGTTGTGTAGTAATACATTGGCTTTGGTTAAGAAATTGACACACACTCATTTTACAAATCAAAAAACCAATAAAATAACCTCTGACCACCCCACAATTGTTCTCTTTTCCCACCCTGTCGATCAGGTATTAATTCTTTTTTCTTTTATTTATTTTATTAATTAAATTATAAATTTTGTATTAAAATTTTGATATTTATTGTGTATATTTGGTTTTTAAATTTGTTTAATACGTCTTTAAACTAACTTTCAATTTTATATTTAATTGGATAGAGGTCTCTAACAAATTCAAAATCTAAAATAATAAATCCATCTTAAAATTGATTTTTTTTTTTTTAATTATTTGAATAGTTAAATAGTAACTTGGTAGTAATATATACAAATAAAATTATTTACATTTTTTACTAATAACTTTTTTTTCTCTAATAAAAAGTATACAAATAAATTGATGAGTTATTATTTGTTAAGATCAAATAAATTTCTTTTCAAAAAAATCAAGTAAACTTATAAATATACAACACTTTTATATCACATAATATTATAATAAATATTCTTGCTCGTTTTTAATTTAATTAAAAATGATAGAAAAAATTACCTTTTTTGTTTTTTTTTTTCCTTTTTTTTTTTTTTCAAGTTTTGTAAAATAGGTGTGTGTTTGGTCCCTAAATTTTCAAAAATACCTTTTTATTCATAAACTTTGAAGAATATGTGTTTGATCTCTACATTTCCAAAATGTACATTTTTAATCCTAAACTTTTAATAACATGAGTGTTTGATTCCTAAGTCTTCAAAATATATTTTTTTAGTTCCTAGGTTTTAAGAGAACATGTTTGAGAGGTTCCTAAAATAATTTTTTATTATTATTTAAATTAAAAAAAGAATTTTAAAAAATTATCTCTTCTCCCTATAAAATTATTTTTGAAAATTAACTTTCTAATTTAAAATTTCATATAATCATTTAAATAGTAACAAATTTAAAATTTTAAACATATTCTTAAAAAATCGAAGAGGAAAAAGGTTCATATTTGTCAAAACTGAAGGACTACAAAATTAATTTTGCCAAAATATATTAGCATATAATACCAATTTTTCTAGAAATATGGAAGCGAAAATTTGCAGTAATAATTAATGAATTTTGATTCCTATCTTGGGTGTAGATATAGGCTTTTGTAGTTAACAACTGCAACACGTTTCTGGATCCAATTATATAGATAGACCATATCTTCCGTGTAGATTAGTTTTGATTTTGATTCTTTGAATAAGACATAAGATTATATTATAGTTTCTTTCTTTTATTGTGTATAAAAAGTTATTATATTGATATAATTATCTAAGTTCATCCTTGATATCGAAACGTAAAGAGTTTGACTAAAACCATATACAAGCTAAGGACTAAATTGAAAATTTTACCCAAACCAAACAACCAAAATTGAAGGGACCAAATTGCAATAACATTATTTAAGTGAAGAAATTTTGGATAATTAATAACAGGAGGAAGGTGAGAATAGAGGAATTAGGGAGGATTTAGAAGACGGAATAAAGTGGCCAGAATGGATGGCGAGCCGTGACCGGAGGCTTTTGGAGTCATCGTACGAAGCTACGGCGGACGTGGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGTCGGAGGCGGTGGCGGCGGCGCCGAGTAAGAGCAGCAAAAGATATGTAATAAGAATAAAGGGTGGAGTTTACAAGGAAAATGTTGATGTGCCAAGTAGTAAAACCAATATTATGTTTTGGGGAGATGGAAGATCCAACACCATTATCACTGCTAGCAGAAGTGTTGGGGGTGGCAGTACAACCTTCAATTCCGCCACAGTTGGTAACACTGCTCAACCACAACTCCTTTTGACTCTTTAATTTTCAAAATAAGTCTGTTTTAGAAAAAATTGAACTACTTTCAGAATCTTTCCAAATATATTTTTAAATCATTTTTATTAAGATTATTTTTTAAGTGCATAGTACGTATAGTACGTTTAGAAATGATTTTGAAAATATTAAATAACCATTTTTGTTTGAAACATGCTCTAATATTTAAAATCAATTTTGATTTTTTTATAATAGTATTTAAAAGTGTAACATTAATTTGATTTTTAATGATTGAATATGTGTTTTAGCTTCATTTTAAACTTGACAAATATGATCATTTTAACTATCTTGAAATCACTTTCAAACAACATTTTCAATATATTTTAAAAGTAATTTTAAAATGGTTAAAATTATTTTAACTTTGTCATTCTCACTCATTACTCAAATTAATTTTGATTTATTAAAATCATGTTTAAAATGGTAAGAAAAAAAATGATTAAATTAATTTTGAATAATTAGAAGTATATTTGAGAATGAGTAATTATGAATATGACAAATCAAAATTGATTTTAACCATTTCAAAATCAACCTCAACTATTTATTTGCCAAACCATTTATTATAATTATATAAAAAACATGCAACTCTTTGAGAACAATGTTGCGCAAAAACACTTTTTAAGATTAAAAACATGACAAACAATAACAATGTTGTTTTTCCTTTGGCAGCTGCTGTGGGGGATGGATTTTTAGCTCGGGATATAACCTTTCAAAACACAGCAGGAGCAGCCAATGACCAAGCTGTAGCTCTCCGCGTTGGGTCAGATCGCTCAGCCTTCTACCGTTGTGGCATGCTAGCCTACCAAGACACTCTCTACGTCCATTCAAACCGTCAGTTCTTCGTCAACTGCGTCGTTGCAGGCACAGTCGATTTCATCTTCGGAAACGCAGCAGTCGTATTCCAAAACTCCGACCTCACCCCTCGCAAACCAGGCCCTAAGCAACAGAACATGGTCACAGCACAAGGAAGAACTGACCCGAATCAAAACACGGGAATCGTGATCCAAAAGTGTAAAATAAAAGCGACGTCGGACTTGGAGCCGGTGATAAAAGAATTCCCAAGCTTCCTTGGGAGGCCATGGAAGGATTATGCTAGGGTTGTGGTAATGCAAACGGACATAACCAATGTGATAGACTCAAAAGGGTGGGCGCCATGGGGTAGTGAAATAAAGAAACCCTACTTTGCGGAATATGATAATAATGGAGCTGGAGCCAATACTTGTTGTAGAGTGACATGGTCTACAATTATTAATGCAGCTCAAGCTAAGACCTTTACGGCTGAACCATTCATAGATGGTGCTAATTGGCTCCCATCCACTGGCTTCCCCTATCAGCTTGGCCTCTAAATCTATTGCTATAATTACCTTGCAACTCTTGGCCTTTTGGTTTGGTTGATCAAAAGATGTATGAGAGTTGGAACATAAGCTAGTAAATGGGAAATGTTTTAATCGAATTAAAATAAAGTTTAGACTTACATATTAAAATCTTGTTTTTTGTTCTGGTAGGTTTATTTTAATTTCTAGCTTTTGATTCATACTTGGGCCTATTTCGAAGATGGAAATGAGATGTGTTTGGAATGGCTGCCTTGTAGTCCAATTACAAGCCCACGTTTGAGGGCTCGAGTTACATTTGAGTTTACATGATTGAATTGGCCCTAAATCCATTCCAATACAAACCTATAAAAGTTTGTTGTAGGTACTTAGTGCCTTATCTAATAAAATATTTTAAAAAAGTGTCTATTAATTTCCTACT

mRNA sequence

AATTATAGGGCTACCAAAGGAACTTCCCTTCTCTCTTTATAACCCACCAACCCTCTCTGCCTCTCTTCACAACTCTTCTCATTTCCGAGCTATGGAAAACACAAAACTTTCATTACCTCTCTTTGCTGCTTTACTCCTCACAAATGCTATTGTTGTTTTAGCCAATTCCAATGAGAATAGTGACATGAATAATAACTCGAGTGTCATAGAAAACTCATGTGCCATTACTCTTTATCCTCAACTATGTCAATCAACAATATACTCCATCGTCGGAACATCCAACTTGTTGAGTTTGAAAGATATTGTTGAAGTGTCATTGAGTGTTGCTGTTGATGCAGCTAAGCATAACTACAAAAGCATCAACAAACTTTGGATGTCACTTAATGATGTTAGTAAACGAGACAAGATTGCGCTTCGTGATTGTGTGGAAACCACAGACAGGGCGATTTATGAACTTCACAAGGCCGTAGAGGATCTTCGTGAGTATCCCAACAAGAAATCTCTTACCTTGTACGCTGATGACCTCAAAACCTTTCTCAGTTCTGCCATTACTAATCAGGAGACTTGTTTGGAGGGATTGTCACATGAGAAAAGGGTGTTGAAGCTGATTGAAAGCACACATATTCACGTCACAAAGTTGTGTAGTAATACATTGGCTTTGGTTAAGAAATTGACACACACTCATTTTACAAATCAAAAAACCAATAAAATAACCTCTGACCACCCCACAATTGTTCTCTTTTCCCACCCTGTCGATCAGGAGGAAGGTGAGAATAGAGGAATTAGGGAGGATTTAGAAGACGGAATAAAGTGGCCAGAATGGATGGCGAGCCGTGACCGGAGGCTTTTGGAGTCATCGTACGAAGCTACGGCGGACGTGGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGTCGGAGGCGGTGGCGGCGGCGCCGAGTAAGAGCAGCAAAAGATATGTAATAAGAATAAAGGGTGGAGTTTACAAGGAAAATGTTGATGTGCCAAGTAGTAAAACCAATATTATGTTTTGGGGAGATGGAAGATCCAACACCATTATCACTGCTAGCAGAAGTGTTGGGGGTGGCAGTACAACCTTCAATTCCGCCACAGTTGCTGCTGTGGGGGATGGATTTTTAGCTCGGGATATAACCTTTCAAAACACAGCAGGAGCAGCCAATGACCAAGCTGTAGCTCTCCGCGTTGGGTCAGATCGCTCAGCCTTCTACCGTTGTGGCATGCTAGCCTACCAAGACACTCTCTACGTCCATTCAAACCGTCAGTTCTTCGTCAACTGCGTCGTTGCAGGCACAGTCGATTTCATCTTCGGAAACGCAGCAGTCGTATTCCAAAACTCCGACCTCACCCCTCGCAAACCAGGCCCTAAGCAACAGAACATGGTCACAGCACAAGGAAGAACTGACCCGAATCAAAACACGGGAATCGTGATCCAAAAGTGTAAAATAAAAGCGACGTCGGACTTGGAGCCGGTGATAAAAGAATTCCCAAGCTTCCTTGGGAGGCCATGGAAGGATTATGCTAGGGTTGTGGTAATGCAAACGGACATAACCAATGTGATAGACTCAAAAGGGTGGGCGCCATGGGGTAGTGAAATAAAGAAACCCTACTTTGCGGAATATGATAATAATGGAGCTGGAGCCAATACTTGTTGTAGAGTGACATGGTCTACAATTATTAATGCAGCTCAAGCTAAGACCTTTACGGCTGAACCATTCATAGATGGTGCTAATTGGCTCCCATCCACTGGCTTCCCCTATCAGCTTGGCCTCTAAATCTATTGCTATAATTACCTTGCAACTCTTGGCCTTTTGGTTTGGTTGATCAAAAGATGTATGAGAGTTGGAACATAAGCTAGTAAATGGGAAATGTTTTAATCGAATTAAAATAAAGTTTAGACTTACATATTAAAATCTTGTTTTTTGTTCTGGTAGGTTTATTTTAATTTCTAGCTTTTGATTCATACTTGGGCCTATTTCGAAGATGGAAATGAGATGTGTTTGGAATGGCTGCCTTGTAGTCCAATTACAAGCCCACGTTTGAGGGCTCGAGTTACATTTGAGTTTACATGATTGAATTGGCCCTAAATCCATTCCAATACAAACCTATAAAAGTTTGTTGTAGGTACTTAGTGCCTTATCTAATAAAATATTTTAAAAAAGTGTCTATTAATTTCCTACT

Coding sequence (CDS)

ATGGAAAACACAAAACTTTCATTACCTCTCTTTGCTGCTTTACTCCTCACAAATGCTATTGTTGTTTTAGCCAATTCCAATGAGAATAGTGACATGAATAATAACTCGAGTGTCATAGAAAACTCATGTGCCATTACTCTTTATCCTCAACTATGTCAATCAACAATATACTCCATCGTCGGAACATCCAACTTGTTGAGTTTGAAAGATATTGTTGAAGTGTCATTGAGTGTTGCTGTTGATGCAGCTAAGCATAACTACAAAAGCATCAACAAACTTTGGATGTCACTTAATGATGTTAGTAAACGAGACAAGATTGCGCTTCGTGATTGTGTGGAAACCACAGACAGGGCGATTTATGAACTTCACAAGGCCGTAGAGGATCTTCGTGAGTATCCCAACAAGAAATCTCTTACCTTGTACGCTGATGACCTCAAAACCTTTCTCAGTTCTGCCATTACTAATCAGGAGACTTGTTTGGAGGGATTGTCACATGAGAAAAGGGTGTTGAAGCTGATTGAAAGCACACATATTCACGTCACAAAGTTGTGTAGTAATACATTGGCTTTGGTTAAGAAATTGACACACACTCATTTTACAAATCAAAAAACCAATAAAATAACCTCTGACCACCCCACAATTGTTCTCTTTTCCCACCCTGTCGATCAGGAGGAAGGTGAGAATAGAGGAATTAGGGAGGATTTAGAAGACGGAATAAAGTGGCCAGAATGGATGGCGAGCCGTGACCGGAGGCTTTTGGAGTCATCGTACGAAGCTACGGCGGACGTGGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGTCGGAGGCGGTGGCGGCGGCGCCGAGTAAGAGCAGCAAAAGATATGTAATAAGAATAAAGGGTGGAGTTTACAAGGAAAATGTTGATGTGCCAAGTAGTAAAACCAATATTATGTTTTGGGGAGATGGAAGATCCAACACCATTATCACTGCTAGCAGAAGTGTTGGGGGTGGCAGTACAACCTTCAATTCCGCCACAGTTGCTGCTGTGGGGGATGGATTTTTAGCTCGGGATATAACCTTTCAAAACACAGCAGGAGCAGCCAATGACCAAGCTGTAGCTCTCCGCGTTGGGTCAGATCGCTCAGCCTTCTACCGTTGTGGCATGCTAGCCTACCAAGACACTCTCTACGTCCATTCAAACCGTCAGTTCTTCGTCAACTGCGTCGTTGCAGGCACAGTCGATTTCATCTTCGGAAACGCAGCAGTCGTATTCCAAAACTCCGACCTCACCCCTCGCAAACCAGGCCCTAAGCAACAGAACATGGTCACAGCACAAGGAAGAACTGACCCGAATCAAAACACGGGAATCGTGATCCAAAAGTGTAAAATAAAAGCGACGTCGGACTTGGAGCCGGTGATAAAAGAATTCCCAAGCTTCCTTGGGAGGCCATGGAAGGATTATGCTAGGGTTGTGGTAATGCAAACGGACATAACCAATGTGATAGACTCAAAAGGGTGGGCGCCATGGGGTAGTGAAATAAAGAAACCCTACTTTGCGGAATATGATAATAATGGAGCTGGAGCCAATACTTGTTGTAGAGTGACATGGTCTACAATTATTAATGCAGCTCAAGCTAAGACCTTTACGGCTGAACCATTCATAGATGGTGCTAATTGGCTCCCATCCACTGGCTTCCCCTATCAGCTTGGCCTCTAA

Protein sequence

MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIVGTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIYELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSHEKRVLKLIESTHIHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIREDLEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAGANTCCRVTWSTIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL
Homology
BLAST of Clc09G09140 vs. NCBI nr
Match: XP_011659194.1 (pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cucumis sativus])

HSP 1 Score: 847.0 bits (2187), Expect = 9.2e-242
Identity = 453/584 (77.57%), Postives = 494/584 (84.59%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MENTKLSL LFA LLLTNAI+V ANSNEN  MNN  SVIE SCAITLYPQLC STI SI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDIVE+SLSVA+DAAK N ++I KL MS N V KRD+IAL DCV+TTDR IY
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA++D  EYPNKKSL  YADDLKTFLSSAITNQ TCL+GLSH   EKRVL+LIE+TH
Sbjct: 121 ELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTH 180

Query: 181 IHVTKLCSNTLALVKKL-THTHFTNQKT-------NKITS-----DHPTIVLFSHPVDQE 240
             VTKLCSN LALVKKL T    T++K+        KITS     D P IVLFS   +QE
Sbjct: 181 NQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFS---NQE 240

Query: 241 EGENRGIREDLEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAP 300
           +GE+R  REDLE+GIKWPEWM+ +D+ LL+SS E  ADVVVAADG+GNYKTVSEAV AAP
Sbjct: 241 DGESRR-REDLENGIKWPEWMSIQDQMLLDSSSEEAADVVVAADGTGNYKTVSEAVKAAP 300

Query: 301 SKSSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVA 360
           SK+S RY+I+IK GVY+ENVDVPSSK NIMFWGDGRSNTIITA RS G G +TFNSATV 
Sbjct: 301 SKNS-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVV 360

Query: 361 AVGDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNC 420
           AVGDGFLARDITFQNTAG+AN QAVALRVGSD SAFYRC MLAYQDTLYVHSNRQFFV C
Sbjct: 361 AVGDGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKC 420

Query: 421 VVAGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATS 480
           VVAGTVDFIFGNAA V QNSDLTPRKPGP Q NMVTAQ RTD NQNTGIVIQKC+IKATS
Sbjct: 421 VVAGTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATS 480

Query: 481 DLEPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAG 540
           DLEPVIKEFPSFLGRPW++YARVVVMQT ITNVID +GW+ W  +IKKPYFAEYDNNGAG
Sbjct: 481 DLEPVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAG 540

Query: 541 ANTCCRVTWSTIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           A+T  RV+WS +IN A+AKTFTAEPFIDGA WLPSTGFPYQLGL
Sbjct: 541 ADTSGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGL 579

BLAST of Clc09G09140 vs. NCBI nr
Match: TYK30782.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 846.3 bits (2185), Expect = 1.6e-241
Identity = 445/573 (77.66%), Postives = 489/573 (85.34%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MENTKLSL LFA LLLTNAI+V ANSNEN  M+N SS+IE SCAITLYPQLC STI SI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDIV++SLSVA+DAAK N  +I KL MS+N+VSKRD+I L DCV++TDR IY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA+ED REYPNKKSLT YADDLKTFLSSAITNQ TCL+G      EKRVL++IE+TH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHVTKLCSNTLALVKKL-THTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIREDLE 240
           IH TKLCSN LALVKKL T     ++K  K +    + VLFS   +QEEGEN G REDLE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLKKSRKARSHVLFS---NQEEGEN-GRREDLE 240

Query: 241 DGIKWPEWMASRDRRLLE-SSYEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIRI 300
           +GIKWPEWM+ +D++LLE SS EA  DVVVA DGSGNYKTVSEAV AAPSK+SKRY+I+I
Sbjct: 241 NGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSKRYIIKI 300

Query: 301 KGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARDI 360
           K GVY+ENVDVPSSK NIMFWGDGRSNTIITA RS G G +TFNSATV AVGDGFLARDI
Sbjct: 301 KAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLARDI 360

Query: 361 TFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIFG 420
           +F+NTAG AN QAVALRVGSD SAFYRCGML YQDTLYVHSNRQFFVNCVVAGTVDF+FG
Sbjct: 361 SFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDFVFG 420

Query: 421 NAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFPS 480
           NAA V QNSDLTPRKPGP Q+NMVTAQ RTD NQNTGIVIQKC+IKATSDLEPVIKEFPS
Sbjct: 421 NAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKEFPS 480

Query: 481 FLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAGANTCCRVTWST 540
           FLGRPW++YARVVVMQT ITNVID KGW+ W  +IKKPYFAEYDNNGAGANT  RV WS 
Sbjct: 481 FLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANTSGRVPWSL 540

Query: 541 IINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           +IN A AKTFTAE FI GA+WLPSTGFPYQLGL
Sbjct: 541 VINEADAKTFTAERFIGGADWLPSTGFPYQLGL 569

BLAST of Clc09G09140 vs. NCBI nr
Match: KAA0026177.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 839.3 bits (2167), Expect = 1.9e-239
Identity = 440/572 (76.92%), Postives = 480/572 (83.92%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MENTKLSL LFA LLLTNAI+V ANSNEN  M+N SS+IE SCAITLYPQLC STI SI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDIV++SLSVA+DAAK N  +I KL MS+N+VSKRD+I L DCV++TDR IY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA+ED REYPNKKSLT YADDLKTFLSSAITNQ TCL+G      EKRVL++IE+TH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIREDLED 240
           IH TKLCSN LALVKKLT        T     D   +         EEGEN G REDLE+
Sbjct: 181 IHATKLCSNALALVKKLT--------TEIALIDEKNLKKSRKARSHEEGEN-GRREDLEN 240

Query: 241 GIKWPEWMASRDRRLLE-SSYEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIRIK 300
           GIKWPEWM+ +D++LLE SS EA  DVVVA DGSGNYKTVSEAV AAPSK+SKRY+I+IK
Sbjct: 241 GIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSKRYIIKIK 300

Query: 301 GGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARDIT 360
            GVY+ENVDVPSSK NIMFWGDGRSNTIITA RS G G +TFNSATV AVGDGFLARDI+
Sbjct: 301 AGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLARDIS 360

Query: 361 FQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIFGN 420
           F+NTAG AN QAVALRVGSD SAFYRCGML YQDTLYVHSNRQFFVNCVVAGTVDF+FGN
Sbjct: 361 FENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDFVFGN 420

Query: 421 AAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFPSF 480
           AA V QNSDLTPRKPGP Q+NMVTAQ RTD NQNTGIVIQKC+IKATSDLEPVIKEFPSF
Sbjct: 421 AAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKEFPSF 480

Query: 481 LGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAGANTCCRVTWSTI 540
           LGRPW++YARVVVMQT ITNVID KGW+ W  +IKKPYFAEYDNNGAGANT  RV WS +
Sbjct: 481 LGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANTSGRVPWSLV 540

Query: 541 INAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           IN A AKTFTAE FI GA+WLPSTGFPYQLGL
Sbjct: 541 INEADAKTFTAERFIGGADWLPSTGFPYQLGL 563

BLAST of Clc09G09140 vs. NCBI nr
Match: XP_008458040.2 (PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo])

HSP 1 Score: 839.0 bits (2166), Expect = 2.5e-239
Identity = 446/585 (76.24%), Postives = 487/585 (83.25%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MENTKLSL LFA LLLTNAI+V ANSNEN  M+N SS+IE SCAITLYPQLC STI SI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDIV++SLSVA+DAAK N  +I  L MS N+VSKRD+I L DCV++TDR IY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNL-MSFNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA+ED REYPNKKSLT YADDLKTFLSSAITNQ TCL+G      EK VL++IE+TH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180

Query: 181 IHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHP-------------TIVLFSHPVDQE 240
           IH TKLCSN LALVKKLT       + N +  D P              IVLFS   +QE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFS---NQE 240

Query: 241 EGENRGIREDLEDGIKWPEWMASRDRRLLE-SSYEATADVVVAADGSGNYKTVSEAVAAA 300
           EGEN G REDLE+GIKWPEWM+ +D++LLE SS EA ADVVVA DGSGNYKTVSEAV AA
Sbjct: 241 EGEN-GRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAA 300

Query: 301 PSKSSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATV 360
           PSK+SKRY+I+IK GVY+ENVDVPSSK NIMFWGDGRSNTIITA RS G G +TFNSATV
Sbjct: 301 PSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATV 360

Query: 361 AAVGDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVN 420
            AVGDGFLARDI+F+NTAG AN QAVALRVGSD SAFYRCGML YQDTLYVHSNRQFFVN
Sbjct: 361 VAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVN 420

Query: 421 CVVAGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKAT 480
           CVVAGTVDF+FGNAA V QNSDLTPRKPGP Q+NMVTAQ RTD NQNTGIVIQKC+IKAT
Sbjct: 421 CVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKAT 480

Query: 481 SDLEPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGA 540
           SDLEPVIKEFPSFLGRPW++YARVVVMQT ITNVID KGW+ W  +IKKPYFAEYDNNGA
Sbjct: 481 SDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGA 540

Query: 541 GANTCCRVTWSTIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           GANT  RV WS +IN A AKTFTAE FI GA+WLPSTGFPYQLGL
Sbjct: 541 GANTSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 580

BLAST of Clc09G09140 vs. NCBI nr
Match: XP_004139620.2 (pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cucumis sativus])

HSP 1 Score: 823.9 bits (2127), Expect = 8.3e-235
Identity = 442/584 (75.68%), Postives = 486/584 (83.22%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MEN KLSL L A LLLTNAIVV ANSNEN       SVIE SCAITLYPQLC STI SIV
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDI EVSLSVA+DAAKHN K+I KL +S N+VSKRDKI L DCVETTDR IY
Sbjct: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA+E  REYPNK+SLTLYADDLKTFLSSAITNQ TCL+GLSH   EKRVL+LIE+ H
Sbjct: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAH 180

Query: 181 IHVTKLCSNTLALVKKL-THTHFTNQKT-------NKITS-----DHPTIVLFSHPVDQE 240
           IHVTKLCSN LALV+KL T    T++K+        KITS     D P IVLFS   +QE
Sbjct: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS---NQE 240

Query: 241 EGENRGIREDLEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAP 300
           E ENR  RE+LEDGIKWP+WM+  D++LLESS EA A+ VVAADGSGNYKTV+EAVAAAP
Sbjct: 241 EDENRR-REELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAP 300

Query: 301 SKSSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVA 360
           SK+SKRY+I+IK G Y ENVDVPSSK NIMFWGDGRSNT I ++RS G G +TF SAT+A
Sbjct: 301 SKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLA 360

Query: 361 AVGDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNC 420
           AVGDGFLARDITFQN AGAAN QAVALRVGSD SAFYRC MLAYQDTLYVHSNRQFFVNC
Sbjct: 361 AVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNC 420

Query: 421 VVAGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATS 480
           +VAGTVDFIFGNAA VFQNSD+TPRKPGP Q+NMVTAQ RTD NQNTGIVIQKC+IKATS
Sbjct: 421 IVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATS 480

Query: 481 DLEPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAG 540
           DLEPVI+EFPSFLGRPW++YARVVVMQT I+NVID +GW+ W  + K PY+AEYDNNGAG
Sbjct: 481 DLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAG 540

Query: 541 ANTCCRVTWSTIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           A+   RV WS +I+ AQAKTFTA PFI GA+WL STGFPYQL L
Sbjct: 541 ADISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 573

BLAST of Clc09G09140 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 9.4e-165
Identity = 320/585 (54.70%), Postives = 410/585 (70.09%), Query Frame = 0

Query: 5   KLSLPLFAALLLTNAIV-VLA-------NSNENSDMNNNSS----VIENSCAITLYPQLC 64
           K+ L + A++LL  A++ V+A       NS++++D+   SS    +++++C+ TL+P+LC
Sbjct: 20  KIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHPELC 79

Query: 65  QSTIYSIVGTS-NLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDC 124
            S I ++   S  + S KD++E+SL++ V A + NY ++ +L  +   ++ R+K+AL DC
Sbjct: 80  YSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALHDC 139

Query: 125 VETTDRAIYELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKR 184
           +ET D  + ELH AVEDL  YPNKKSL  + +DLKT +SSAITNQETCL+G SH   +K+
Sbjct: 140 LETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDEADKK 199

Query: 185 VLKLIESTHIHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGEN 244
           V K++     HV K+CSN LA++  +T T   N+            +  S P +      
Sbjct: 200 VRKVLLKGQKHVEKMCSNALAMICNMTDTDIANE------------MKLSAPANN----- 259

Query: 245 RGIREDLEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSKSS 304
              R+ +ED  +WPEW+++ DRRLL+SS   T DVVVAADGSG+YKTVSEAV  AP KSS
Sbjct: 260 ---RKLVEDNGEWPEWLSAGDRRLLQSS-TVTPDVVVAADGSGDYKTVSEAVRKAPEKSS 319

Query: 305 KRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGD 364
           KRYVIRIK GVY+ENVDVP  KTNIMF GDG+SNTIITASR+V  GSTTF+SATV  V  
Sbjct: 320 KRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAG 379

Query: 365 GFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAG 424
             LARDITFQNTAGA+  QAVAL VGSD SAFYRC MLAYQDTLYVHSNRQFFV C+VAG
Sbjct: 380 KVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAG 439

Query: 425 TVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEP 484
           TVDFIFGN A VFQ+ D+  R+PG  Q+NMVTAQGRTDPNQNTGIVIQKC+I ATSDL P
Sbjct: 440 TVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRP 499

Query: 485 VIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSE--IKKPYFAEYDNNGAGAN 544
           V K FP++LGRPWK+Y+R V+MQ+ IT+VI   GW  W     +   ++ EY N GAGA 
Sbjct: 500 VQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAP 559

Query: 545 TCCRVTW---STIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           T  RV W     I ++ +A+ +T   FI G +WL STGFP+ LGL
Sbjct: 560 TSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of Clc09G09140 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 8.8e-163
Identity = 312/579 (53.89%), Postives = 397/579 (68.57%), Query Frame = 0

Query: 5   KLSLPLFAALLLTNAIVVL---ANSNENSDMNNNS---SVIENSCAITLYPQLCQSTIYS 64
           KL L LFA LL+  A++ +    NS +NS  N N    +++++SC+ T YP LC S I +
Sbjct: 29  KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAA 88

Query: 65  IVGTS-NLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDR 124
           +   S  + S KD++E+SL++   A +HNY  I KL +   +++KR+K+AL DC+ET D 
Sbjct: 89  VPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHDCLETIDE 148

Query: 125 AIYELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSHE---KRVLKLIE 184
            + ELHKAVEDL EYPNKKSL+ +ADDLKT +S+A+TNQ TCL+G SH+   K V   + 
Sbjct: 149 TLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALS 208

Query: 185 STHIHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIRED 244
              +HV K+CSN LA++K +T T     +T+                      NR + E+
Sbjct: 209 DGQVHVEKMCSNALAMIKNMTDTDMMIMRTS---------------------NNRKLTEE 268

Query: 245 LEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIR 304
                 WP W++  DRRLL+SS   T + VVAADGSGN+KTV+ AVAAAP   +KRY+IR
Sbjct: 269 TSTVDGWPAWLSPGDRRLLQSS-SVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIR 328

Query: 305 IKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARD 364
           IK GVY+ENV+V     NIMF GDGR+ TIIT SR+V  GSTTF SAT A VG+GFLARD
Sbjct: 329 IKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARD 388

Query: 365 ITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIF 424
           ITFQNTAG +  QAVALRVG+D SAFY C MLAYQDTLYVHSNRQFFVNC++AGTVDFIF
Sbjct: 389 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 448

Query: 425 GNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFP 484
           GNAA V QN D+  RKP   Q+NMVTAQGRTDPNQNTGIVIQK +I ATSDL+PV   FP
Sbjct: 449 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFP 508

Query: 485 SFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSE--IKKPYFAEYDNNGAGANTCCRVT 544
           ++LGRPWK+Y+R V+MQ+ IT++I   GW  W     +   ++ E+ N+GAGA T  RV 
Sbjct: 509 TYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVK 568

Query: 545 WS---TIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           W     I +A +A+ FT   FI G++WL STGFP+ LGL
Sbjct: 569 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Clc09G09140 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 8.8e-163
Identity = 313/579 (54.06%), Postives = 398/579 (68.74%), Query Frame = 0

Query: 5   KLSLPLFAALLLTNAIVVL---ANSNENSDMNNNS---SVIENSCAITLYPQLCQSTIYS 64
           KL L LFA LL+  A++ +    NS +NS  N N    +++++SC+ T YP LC S I +
Sbjct: 29  KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAA 88

Query: 65  IVGTS-NLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDR 124
           +   S  + S KD++E+SL++   A +HNY  I KL +   +++KR+K+AL DC+ET D 
Sbjct: 89  VPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHDCLETIDE 148

Query: 125 AIYELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSHE---KRVLKLIE 184
            + ELHKAVEDL EYPNKKSL+ +ADDLKT +S+A+TNQ TCL+G SH+   K V   + 
Sbjct: 149 TLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALS 208

Query: 185 STHIHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIRED 244
              +HV K+CSN LA++K +T T     +T+                      NR + E+
Sbjct: 209 DGQVHVEKMCSNALAMIKNMTDTDMMIMRTS---------------------NNRKLIEE 268

Query: 245 LEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIR 304
                 WP W+++ DRRLL+SS   T +VVVAADGSGN+KTV+ +VAAAP   +KRY+IR
Sbjct: 269 TSTVDGWPAWLSTGDRRLLQSS-SVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIR 328

Query: 305 IKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARD 364
           IK GVY+ENV+V     NIMF GDGR+ TIIT SR+V  GSTTF SATVA VG+GFLARD
Sbjct: 329 IKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARD 388

Query: 365 ITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIF 424
           ITFQNTAG +  QAVALRVG+D SAFY C MLAYQDTLYVHSNRQFFVNC++AGTVDFIF
Sbjct: 389 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 448

Query: 425 GNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFP 484
           GNAA V QN D+  RKP   Q+NMVTAQGR DPNQNTGIVIQK +I ATSDL+PV   FP
Sbjct: 449 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFP 508

Query: 485 SFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSE--IKKPYFAEYDNNGAGANTCCRVT 544
           ++LGRPWK+Y+R V+MQ+ IT+VI   GW  W     +   ++ E+ N GAGA T  RV 
Sbjct: 509 TYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVK 568

Query: 545 WS---TIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           W     I +A +A+ FT   FI G++WL STGFP+ LGL
Sbjct: 569 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Clc09G09140 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 557.0 bits (1434), Expect = 2.5e-157
Identity = 311/587 (52.98%), Postives = 398/587 (67.80%), Query Frame = 0

Query: 2   ENTKLSLPLFAALLLTNAIVV-----LANSNENSDMNNNS-SVIENSCAITLYPQLCQST 61
           +N KL L   A  LL  A V       + +NE   ++ +S +V+ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  IYSIVGTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETT 121
           + +  G   L S KD++E S+++ + A +HNY ++ KL      ++ R+K AL DC+ET 
Sbjct: 76  VVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 DRAIYELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKL 181
           D  + ELH+ VEDL  YP KK+L  +A DLKT +SSAITNQETCL+G SH   +K+V K 
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 IESTHIHVTKLCSNTLALVKKLTHTHFTN-QKTNKITSDHPTIVLFSHPVDQEEGENRGI 241
           +    IHV  +CSN LA++K +T T   N ++  KITS++  +        +EE +   +
Sbjct: 196 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKL--------KEENQETTV 255

Query: 242 REDLE-----DGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSK 301
             D+      D   WP W+++ DRRLL+ S    AD  VAADGSG +KTV+ AVAAAP  
Sbjct: 256 AVDIAGAGELDSEGWPTWLSAGDRRLLQGS-GVKADATVAADGSGTFKTVAAAVAAAPEN 315

Query: 302 SSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAV 361
           S+KRYVI IK GVY+ENV+V   K NIMF GDGR+ TIIT SR+V  GSTTF+SATVAAV
Sbjct: 316 SNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 375

Query: 362 GDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVV 421
           G+ FLARDITFQNTAG +  QAVALRVGSD SAFY C MLAYQDTLYVHSNRQFFV C++
Sbjct: 376 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 435

Query: 422 AGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDL 481
           AGTVDFIFGNAAVV Q+ D+  R+P   Q+NMVTAQGRTDPNQNTGIVIQKC+I ATSDL
Sbjct: 436 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 495

Query: 482 EPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGS--EIKKPYFAEYDNNGAG 541
           + V   FP++LGRPWK+Y++ V+MQ+ I++VI  +GW+ W     +    + EY N GAG
Sbjct: 496 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAG 555

Query: 542 ANTCCRVTW---STIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           A T  RV W     I  AA+A+ +TA  FI G  WL STGFP+ LGL
Sbjct: 556 AGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Clc09G09140 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 533.9 bits (1374), Expect = 2.2e-150
Identity = 304/586 (51.88%), Postives = 385/586 (65.70%), Query Frame = 0

Query: 3   NTKLSLPLFA-ALLLTNAIVVLA----NSNENSDMNNNSS----VIENSCAITLYPQLCQ 62
           N KL L   A ALLL  +IV +A    N N+N  +   SS    ++++ C+ TLYP+LC 
Sbjct: 17  NKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCF 76

Query: 63  STIYSIVGTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVE 122
           S + +  G   L S K+++E SL++   A KHNY ++ KL      ++ R+  AL DC+E
Sbjct: 77  SAV-AATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLE 136

Query: 123 TTDRAIYELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVL 182
           T D  + ELH AVEDL +YP +KSL  +ADDLKT +SSAITNQ TCL+G S+   +++V 
Sbjct: 137 TIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVR 196

Query: 183 KLIESTHIHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRG 242
           K +    +HV  +CSN LA++K +T T   N +    +S                  NR 
Sbjct: 197 KALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF------------TNNNNRK 256

Query: 243 IRE---DLEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSKS 302
           ++E   DL D   WP+W++  DRRLL+ S    AD  VA DGSG++ TV+ AVAAAP KS
Sbjct: 257 LKEVTGDL-DSDGWPKWLSVGDRRLLQGS-TIKADATVADDGSGDFTTVAAAVAAAPEKS 316

Query: 303 SKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVG 362
           +KR+VI IK GVY+ENV+V   KTNIMF GDGR  TIIT SR+V  GSTTF+SATVAAVG
Sbjct: 317 NKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVG 376

Query: 363 DGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVA 422
           + FLARDITFQNTAG +  QAVALRVGSD SAFY+C M AYQDTLYVHSNRQFFV C + 
Sbjct: 377 ERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHIT 436

Query: 423 GTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLE 482
           GTVDFIFGNAA V Q+ D+  R+P   Q+NMVTAQGR+DPNQNTGIVIQ C+I  TSDL 
Sbjct: 437 GTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLL 496

Query: 483 PVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSE--IKKPYFAEYDNNGAGA 542
            V   FP++LGRPWK+Y+R V+MQ+DI++VI  +GW  W     +    + EY N G GA
Sbjct: 497 AVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGA 556

Query: 543 NTCCRVTW---STIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
            T  RV W     I +  +A+ FTA  FI G  WL STGFP+ L L
Sbjct: 557 GTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Clc09G09140 vs. ExPASy TrEMBL
Match: A0A0A0K6S6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 4.4e-242
Identity = 453/584 (77.57%), Postives = 494/584 (84.59%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MENTKLSL LFA LLLTNAI+V ANSNEN  MNN  SVIE SCAITLYPQLC STI SI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDIVE+SLSVA+DAAK N ++I KL MS N V KRD+IAL DCV+TTDR IY
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA++D  EYPNKKSL  YADDLKTFLSSAITNQ TCL+GLSH   EKRVL+LIE+TH
Sbjct: 121 ELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTH 180

Query: 181 IHVTKLCSNTLALVKKL-THTHFTNQKT-------NKITS-----DHPTIVLFSHPVDQE 240
             VTKLCSN LALVKKL T    T++K+        KITS     D P IVLFS   +QE
Sbjct: 181 NQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFS---NQE 240

Query: 241 EGENRGIREDLEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAP 300
           +GE+R  REDLE+GIKWPEWM+ +D+ LL+SS E  ADVVVAADG+GNYKTVSEAV AAP
Sbjct: 241 DGESRR-REDLENGIKWPEWMSIQDQMLLDSSSEEAADVVVAADGTGNYKTVSEAVKAAP 300

Query: 301 SKSSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVA 360
           SK+S RY+I+IK GVY+ENVDVPSSK NIMFWGDGRSNTIITA RS G G +TFNSATV 
Sbjct: 301 SKNS-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVV 360

Query: 361 AVGDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNC 420
           AVGDGFLARDITFQNTAG+AN QAVALRVGSD SAFYRC MLAYQDTLYVHSNRQFFV C
Sbjct: 361 AVGDGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKC 420

Query: 421 VVAGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATS 480
           VVAGTVDFIFGNAA V QNSDLTPRKPGP Q NMVTAQ RTD NQNTGIVIQKC+IKATS
Sbjct: 421 VVAGTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATS 480

Query: 481 DLEPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAG 540
           DLEPVIKEFPSFLGRPW++YARVVVMQT ITNVID +GW+ W  +IKKPYFAEYDNNGAG
Sbjct: 481 DLEPVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAG 540

Query: 541 ANTCCRVTWSTIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           A+T  RV+WS +IN A+AKTFTAEPFIDGA WLPSTGFPYQLGL
Sbjct: 541 ADTSGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGL 579

BLAST of Clc09G09140 vs. ExPASy TrEMBL
Match: A0A5D3E4E8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G001330 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 7.6e-242
Identity = 445/573 (77.66%), Postives = 489/573 (85.34%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MENTKLSL LFA LLLTNAI+V ANSNEN  M+N SS+IE SCAITLYPQLC STI SI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDIV++SLSVA+DAAK N  +I KL MS+N+VSKRD+I L DCV++TDR IY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA+ED REYPNKKSLT YADDLKTFLSSAITNQ TCL+G      EKRVL++IE+TH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHVTKLCSNTLALVKKL-THTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIREDLE 240
           IH TKLCSN LALVKKL T     ++K  K +    + VLFS   +QEEGEN G REDLE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLKKSRKARSHVLFS---NQEEGEN-GRREDLE 240

Query: 241 DGIKWPEWMASRDRRLLE-SSYEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIRI 300
           +GIKWPEWM+ +D++LLE SS EA  DVVVA DGSGNYKTVSEAV AAPSK+SKRY+I+I
Sbjct: 241 NGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSKRYIIKI 300

Query: 301 KGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARDI 360
           K GVY+ENVDVPSSK NIMFWGDGRSNTIITA RS G G +TFNSATV AVGDGFLARDI
Sbjct: 301 KAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLARDI 360

Query: 361 TFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIFG 420
           +F+NTAG AN QAVALRVGSD SAFYRCGML YQDTLYVHSNRQFFVNCVVAGTVDF+FG
Sbjct: 361 SFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDFVFG 420

Query: 421 NAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFPS 480
           NAA V QNSDLTPRKPGP Q+NMVTAQ RTD NQNTGIVIQKC+IKATSDLEPVIKEFPS
Sbjct: 421 NAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKEFPS 480

Query: 481 FLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAGANTCCRVTWST 540
           FLGRPW++YARVVVMQT ITNVID KGW+ W  +IKKPYFAEYDNNGAGANT  RV WS 
Sbjct: 481 FLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANTSGRVPWSL 540

Query: 541 IINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           +IN A AKTFTAE FI GA+WLPSTGFPYQLGL
Sbjct: 541 VINEADAKTFTAERFIGGADWLPSTGFPYQLGL 569

BLAST of Clc09G09140 vs. ExPASy TrEMBL
Match: A0A5A7SNA2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 9.2e-240
Identity = 440/572 (76.92%), Postives = 480/572 (83.92%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MENTKLSL LFA LLLTNAI+V ANSNEN  M+N SS+IE SCAITLYPQLC STI SI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDIV++SLSVA+DAAK N  +I KL MS+N+VSKRD+I L DCV++TDR IY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA+ED REYPNKKSLT YADDLKTFLSSAITNQ TCL+G      EKRVL++IE+TH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIREDLED 240
           IH TKLCSN LALVKKLT        T     D   +         EEGEN G REDLE+
Sbjct: 181 IHATKLCSNALALVKKLT--------TEIALIDEKNLKKSRKARSHEEGEN-GRREDLEN 240

Query: 241 GIKWPEWMASRDRRLLE-SSYEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIRIK 300
           GIKWPEWM+ +D++LLE SS EA  DVVVA DGSGNYKTVSEAV AAPSK+SKRY+I+IK
Sbjct: 241 GIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSKRYIIKIK 300

Query: 301 GGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARDIT 360
            GVY+ENVDVPSSK NIMFWGDGRSNTIITA RS G G +TFNSATV AVGDGFLARDI+
Sbjct: 301 AGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLARDIS 360

Query: 361 FQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIFGN 420
           F+NTAG AN QAVALRVGSD SAFYRCGML YQDTLYVHSNRQFFVNCVVAGTVDF+FGN
Sbjct: 361 FENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDFVFGN 420

Query: 421 AAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFPSF 480
           AA V QNSDLTPRKPGP Q+NMVTAQ RTD NQNTGIVIQKC+IKATSDLEPVIKEFPSF
Sbjct: 421 AAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKEFPSF 480

Query: 481 LGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAGANTCCRVTWSTI 540
           LGRPW++YARVVVMQT ITNVID KGW+ W  +IKKPYFAEYDNNGAGANT  RV WS +
Sbjct: 481 LGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANTSGRVPWSLV 540

Query: 541 INAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           IN A AKTFTAE FI GA+WLPSTGFPYQLGL
Sbjct: 541 INEADAKTFTAERFIGGADWLPSTGFPYQLGL 563

BLAST of Clc09G09140 vs. ExPASy TrEMBL
Match: A0A1S3C861 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 1.2e-239
Identity = 446/585 (76.24%), Postives = 487/585 (83.25%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MENTKLSL LFA LLLTNAI+V ANSNEN  M+N SS+IE SCAITLYPQLC STI SI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDIV++SLSVA+DAAK N  +I  L MS N+VSKRD+I L DCV++TDR IY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNL-MSFNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA+ED REYPNKKSLT YADDLKTFLSSAITNQ TCL+G      EK VL++IE+TH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180

Query: 181 IHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHP-------------TIVLFSHPVDQE 240
           IH TKLCSN LALVKKLT       + N +  D P              IVLFS   +QE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFS---NQE 240

Query: 241 EGENRGIREDLEDGIKWPEWMASRDRRLLE-SSYEATADVVVAADGSGNYKTVSEAVAAA 300
           EGEN G REDLE+GIKWPEWM+ +D++LLE SS EA ADVVVA DGSGNYKTVSEAV AA
Sbjct: 241 EGEN-GRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAA 300

Query: 301 PSKSSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATV 360
           PSK+SKRY+I+IK GVY+ENVDVPSSK NIMFWGDGRSNTIITA RS G G +TFNSATV
Sbjct: 301 PSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATV 360

Query: 361 AAVGDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVN 420
            AVGDGFLARDI+F+NTAG AN QAVALRVGSD SAFYRCGML YQDTLYVHSNRQFFVN
Sbjct: 361 VAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVN 420

Query: 421 CVVAGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKAT 480
           CVVAGTVDF+FGNAA V QNSDLTPRKPGP Q+NMVTAQ RTD NQNTGIVIQKC+IKAT
Sbjct: 421 CVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKAT 480

Query: 481 SDLEPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGA 540
           SDLEPVIKEFPSFLGRPW++YARVVVMQT ITNVID KGW+ W  +IKKPYFAEYDNNGA
Sbjct: 481 SDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGA 540

Query: 541 GANTCCRVTWSTIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           GANT  RV WS +IN A AKTFTAE FI GA+WLPSTGFPYQLGL
Sbjct: 541 GANTSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 580

BLAST of Clc09G09140 vs. ExPASy TrEMBL
Match: A0A0A0K9Y3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 4.0e-235
Identity = 442/584 (75.68%), Postives = 486/584 (83.22%), Query Frame = 0

Query: 1   MENTKLSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIV 60
           MEN KLSL L A LLLTNAIVV ANSNEN       SVIE SCAITLYPQLC STI SIV
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60

Query: 61  GTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIY 120
           GTSNLLSLKDI EVSLSVA+DAAKHN K+I KL +S N+VSKRDKI L DCVETTDR IY
Sbjct: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120

Query: 121 ELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKLIESTH 180
           EL KA+E  REYPNK+SLTLYADDLKTFLSSAITNQ TCL+GLSH   EKRVL+LIE+ H
Sbjct: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAH 180

Query: 181 IHVTKLCSNTLALVKKL-THTHFTNQKT-------NKITS-----DHPTIVLFSHPVDQE 240
           IHVTKLCSN LALV+KL T    T++K+        KITS     D P IVLFS   +QE
Sbjct: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS---NQE 240

Query: 241 EGENRGIREDLEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAP 300
           E ENR  RE+LEDGIKWP+WM+  D++LLESS EA A+ VVAADGSGNYKTV+EAVAAAP
Sbjct: 241 EDENRR-REELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAP 300

Query: 301 SKSSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVA 360
           SK+SKRY+I+IK G Y ENVDVPSSK NIMFWGDGRSNT I ++RS G G +TF SAT+A
Sbjct: 301 SKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLA 360

Query: 361 AVGDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNC 420
           AVGDGFLARDITFQN AGAAN QAVALRVGSD SAFYRC MLAYQDTLYVHSNRQFFVNC
Sbjct: 361 AVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNC 420

Query: 421 VVAGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATS 480
           +VAGTVDFIFGNAA VFQNSD+TPRKPGP Q+NMVTAQ RTD NQNTGIVIQKC+IKATS
Sbjct: 421 IVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATS 480

Query: 481 DLEPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSEIKKPYFAEYDNNGAG 540
           DLEPVI+EFPSFLGRPW++YARVVVMQT I+NVID +GW+ W  + K PY+AEYDNNGAG
Sbjct: 481 DLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAG 540

Query: 541 ANTCCRVTWSTIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           A+   RV WS +I+ AQAKTFTA PFI GA+WL STGFPYQL L
Sbjct: 541 ADISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 573

BLAST of Clc09G09140 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 557.0 bits (1434), Expect = 1.8e-158
Identity = 311/587 (52.98%), Postives = 398/587 (67.80%), Query Frame = 0

Query: 2   ENTKLSLPLFAALLLTNAIVV-----LANSNENSDMNNNS-SVIENSCAITLYPQLCQST 61
           +N KL L   A  LL  A V       + +NE   ++ +S +V+ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  IYSIVGTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETT 121
           + +  G   L S KD++E S+++ + A +HNY ++ KL      ++ R+K AL DC+ET 
Sbjct: 76  VVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 DRAIYELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVLKL 181
           D  + ELH+ VEDL  YP KK+L  +A DLKT +SSAITNQETCL+G SH   +K+V K 
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 IESTHIHVTKLCSNTLALVKKLTHTHFTN-QKTNKITSDHPTIVLFSHPVDQEEGENRGI 241
           +    IHV  +CSN LA++K +T T   N ++  KITS++  +        +EE +   +
Sbjct: 196 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKL--------KEENQETTV 255

Query: 242 REDLE-----DGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSK 301
             D+      D   WP W+++ DRRLL+ S    AD  VAADGSG +KTV+ AVAAAP  
Sbjct: 256 AVDIAGAGELDSEGWPTWLSAGDRRLLQGS-GVKADATVAADGSGTFKTVAAAVAAAPEN 315

Query: 302 SSKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAV 361
           S+KRYVI IK GVY+ENV+V   K NIMF GDGR+ TIIT SR+V  GSTTF+SATVAAV
Sbjct: 316 SNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 375

Query: 362 GDGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVV 421
           G+ FLARDITFQNTAG +  QAVALRVGSD SAFY C MLAYQDTLYVHSNRQFFV C++
Sbjct: 376 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 435

Query: 422 AGTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDL 481
           AGTVDFIFGNAAVV Q+ D+  R+P   Q+NMVTAQGRTDPNQNTGIVIQKC+I ATSDL
Sbjct: 436 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 495

Query: 482 EPVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGS--EIKKPYFAEYDNNGAG 541
           + V   FP++LGRPWK+Y++ V+MQ+ I++VI  +GW+ W     +    + EY N GAG
Sbjct: 496 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAG 555

Query: 542 ANTCCRVTW---STIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
           A T  RV W     I  AA+A+ +TA  FI G  WL STGFP+ LGL
Sbjct: 556 AGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Clc09G09140 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 533.9 bits (1374), Expect = 1.6e-151
Identity = 304/586 (51.88%), Postives = 385/586 (65.70%), Query Frame = 0

Query: 3   NTKLSLPLFA-ALLLTNAIVVLA----NSNENSDMNNNSS----VIENSCAITLYPQLCQ 62
           N KL L   A ALLL  +IV +A    N N+N  +   SS    ++++ C+ TLYP+LC 
Sbjct: 17  NKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCF 76

Query: 63  STIYSIVGTSNLLSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVE 122
           S + +  G   L S K+++E SL++   A KHNY ++ KL      ++ R+  AL DC+E
Sbjct: 77  SAV-AATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLE 136

Query: 123 TTDRAIYELHKAVEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSH---EKRVL 182
           T D  + ELH AVEDL +YP +KSL  +ADDLKT +SSAITNQ TCL+G S+   +++V 
Sbjct: 137 TIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVR 196

Query: 183 KLIESTHIHVTKLCSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRG 242
           K +    +HV  +CSN LA++K +T T   N +    +S                  NR 
Sbjct: 197 KALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF------------TNNNNRK 256

Query: 243 IRE---DLEDGIKWPEWMASRDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSKS 302
           ++E   DL D   WP+W++  DRRLL+ S    AD  VA DGSG++ TV+ AVAAAP KS
Sbjct: 257 LKEVTGDL-DSDGWPKWLSVGDRRLLQGS-TIKADATVADDGSGDFTTVAAAVAAAPEKS 316

Query: 303 SKRYVIRIKGGVYKENVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVG 362
           +KR+VI IK GVY+ENV+V   KTNIMF GDGR  TIIT SR+V  GSTTF+SATVAAVG
Sbjct: 317 NKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVG 376

Query: 363 DGFLARDITFQNTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVA 422
           + FLARDITFQNTAG +  QAVALRVGSD SAFY+C M AYQDTLYVHSNRQFFV C + 
Sbjct: 377 ERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHIT 436

Query: 423 GTVDFIFGNAAVVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLE 482
           GTVDFIFGNAA V Q+ D+  R+P   Q+NMVTAQGR+DPNQNTGIVIQ C+I  TSDL 
Sbjct: 437 GTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLL 496

Query: 483 PVIKEFPSFLGRPWKDYARVVVMQTDITNVIDSKGWAPWGSE--IKKPYFAEYDNNGAGA 542
            V   FP++LGRPWK+Y+R V+MQ+DI++VI  +GW  W     +    + EY N G GA
Sbjct: 497 AVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGA 556

Query: 543 NTCCRVTW---STIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
            T  RV W     I +  +A+ FTA  FI G  WL STGFP+ L L
Sbjct: 557 GTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Clc09G09140 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 365.2 bits (936), Expect = 9.8e-101
Identity = 236/567 (41.62%), Postives = 322/567 (56.79%), Query Frame = 0

Query: 10  LFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIVGTS-NLLSL 69
           +  ++    A ++  N+N N D    +S I   C        CQ+ +      S + L+ 
Sbjct: 29  ILGSVAFFTAQLISVNTNNNDDSLLTTSQI---CHGAHDQDSCQALLSEFTTLSLSKLNR 88

Query: 70  KDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIYELHKAVED 129
            D++ V L  +V   +     +++  +  N V  RDK    DC E  D +   +  ++E+
Sbjct: 89  LDLLHVFLKNSVWRLESTMTMVSEARIRSNGV--RDKAGFADCEEMMDVSKDRMMSSMEE 148

Query: 130 LREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLSHEKRVLKLIESTHIHVTKLCSNTL 189
           LR      +L  Y+ ++ T+LSS +TN  TCLE +S                  +  N+ 
Sbjct: 149 LR--GGNYNLESYS-NVHTWLSSVLTNYMTCLESIS-----------------DVSVNSK 208

Query: 190 ALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIREDLEDGI--KWPEWMA 249
            +VK         Q  + ++     + +F   +          R+DL+  I  ++P W+ 
Sbjct: 209 QIVKP--------QLEDLVSRARVALAIFVSVLP--------ARDDLKMIISNRFPSWLT 268

Query: 250 SRDRRLLESS---YEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIRIKGGVYKEN 309
           + DR+LLESS    + TA+VVVA DG+G +KTV+EAVAAAP  S+ RYVI +K GVYKE 
Sbjct: 269 ALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKET 328

Query: 310 VDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARDITFQNTAGA 369
           +D+   K N+M  GDG+  TIIT S +V  GSTTF SATVAA GDGF+A+DI FQNTAG 
Sbjct: 329 IDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGP 388

Query: 370 ANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIFGNAAVVFQN 429
           A  QAVALRV +D++   RC + AYQDTLY H+ RQF+ +  + GTVDFIFGN+AVVFQN
Sbjct: 389 AKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQN 448

Query: 430 SDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFPSFLGRPWKD 489
            D+  R PG  Q+NM+TAQGR D NQNT I IQKCKI A+SDL PV     +FLGRPWK 
Sbjct: 449 CDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKL 508

Query: 490 YARVVVMQTDITNVIDSKGWAPWGSE--IKKPYFAEYDNNGAGANTCCRVTW---STIIN 549
           Y+R V+MQ+ I N ID  GW PW  E  +   Y+ EY N G GA+T  RV W     I +
Sbjct: 509 YSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKD 554

Query: 550 AAQAKTFTAEPFIDGANWLPSTGFPYQ 566
           + +A+ FT    I G  WL  TG  +Q
Sbjct: 569 SKEAEQFTVAKLIQGGLWLKPTGVTFQ 554

BLAST of Clc09G09140 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 362.5 bits (929), Expect = 6.4e-100
Identity = 229/566 (40.46%), Postives = 329/566 (58.13%), Query Frame = 0

Query: 11  FAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIVGTSNLLSLKD 70
           F A ++   I+ +  ++  S  N     ++  C+ T  P+ C+  +        + S  +
Sbjct: 4   FRAYIINFVILCILVASTVSGYNQKD--VKAWCSQTPNPKPCEYFLTHNSNNEPIKSESE 63

Query: 71  IVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIYELHKAVEDLR 130
            +++S+ + +D A         L     D   R+K A  DC++  D  + ++++ ++   
Sbjct: 64  FLKISMKLVLDRAILAKTHAFTLGPKCRDT--REKAAWEDCIKLYDLTVSKINETMD--- 123

Query: 131 EYPNKKSLTLYADDLKTFLSSAITNQETCLEG---LSHEKRVLKLIESTHIHVTKLCSNT 190
             PN K   L   D +T+LS+A+TN +TC  G   L     VL L+ +   +V+ L  NT
Sbjct: 124 --PNVKCSKL---DAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSN---NVSNLLCNT 183

Query: 191 LALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEGENRGIREDLEDGIKWPEWMAS 250
           LA+ K                      V F++   +++G              +P W+  
Sbjct: 184 LAINK----------------------VPFNYTPPEKDG--------------FPSWVKP 243

Query: 251 RDRRLLESSYEATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIRIKGGVYKENVDVP 310
            DR+LL+SS     + VVA DGSGN+KT+ EA+ AA    S R+VI +K GVY EN+++ 
Sbjct: 244 GDRKLLQSS-TPKDNAVVAKDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI- 303

Query: 311 SSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARDITFQNTAGAANDQ 370
             K N+M  GDG   TIIT S+SVGGG+TTFNSATVAAVGDGF+AR ITF+NTAGA+N+Q
Sbjct: 304 -RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQ 363

Query: 371 AVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIFGNAAVVFQNSDLT 430
           AVALR GSD S FY+C   AYQDTLYVHSNRQF+ +C V GTVDFIFGNAA V QN ++ 
Sbjct: 364 AVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIF 423

Query: 431 PRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFPSFLGRPWKDYARV 490
            R+P  K  N +TAQGR+DPNQNTGI+I   ++ A SDL PV+    ++LGRPW+ Y+R 
Sbjct: 424 ARRPRSK-TNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRT 483

Query: 491 VVMQTDITNVIDSKGWAPWGSE--IKKPYFAEYDNNGAGANTCCRVTW---STIINAAQA 550
           V M+T + ++ID +GW  W     +K  ++AE+ N G GA+T  RVTW     + +A++A
Sbjct: 484 VFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEA 511

Query: 551 KTFTAEPFIDGANWLPSTGFPYQLGL 569
             FT   F+ G +W+PS+  P+  GL
Sbjct: 544 SKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Clc09G09140 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 344.4 bits (882), Expect = 1.8e-94
Identity = 215/575 (37.39%), Postives = 315/575 (54.78%), Query Frame = 0

Query: 6   LSLPLFAALLLTNAIVVLANSNENSDMNNNSSVIENSCAITLYPQLCQSTIYSIVGTSNL 65
           +SL L AA+       +  N +        S  I  +C +T +P+LC  ++    G+   
Sbjct: 54  ISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAA 113

Query: 66  LSLKDIVEVSLSVAVDAAKHNYKSINKLWMSLNDVSKRDKIALRDCVETTDRAIYELHKA 125
            S KD++ V++++ +    H   S   L  S  D+  R + A   CVE  D ++  L +A
Sbjct: 114 SSSKDLIHVTVNMTLHHFSHALYSSASL--SFVDMPPRARSAYDSCVELLDDSVDALSRA 173

Query: 126 VEDLREYPNKKSLTLYADDLKTFLSSAITNQETCLEGLS--HEKRVLKLIESTHIHVTKL 185
           +  +       S +    D+ T+LS+A+TN +TC EG     +  V   + +   ++++L
Sbjct: 174 LSSV------VSSSAKPQDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSEL 233

Query: 186 CSNTLALVKKLTHTHFTNQKTNKITSDHPTIVLFSHPVDQEEG---ENRGIREDLEDGIK 245
            SN LA+                           SH  D   G   +NR +    E   K
Sbjct: 234 VSNCLAIFSA------------------------SHDGDDFAGVPIQNRRLLGVEEREEK 293

Query: 246 WPEWMASRDRRLLESSY-EATADVVVAADGSGNYKTVSEAVAAAPSKSSKRYVIRIKGGV 305
           +P WM  ++R +LE    +  AD++V+ DG+G  KT+SEA+  AP  S++R +I +K G 
Sbjct: 294 FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGR 353

Query: 306 YKE-NVDVPSSKTNIMFWGDGRSNTIITASRSVGGGSTTFNSATVAAVGDGFLARDITFQ 365
           Y+E N+ V   K N+MF GDG+  T+I+  +S+    TTF++A+ AA G GF+ARDITF+
Sbjct: 354 YEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFE 413

Query: 366 NTAGAANDQAVALRVGSDRSAFYRCGMLAYQDTLYVHSNRQFFVNCVVAGTVDFIFGNAA 425
           N AG A  QAVALR+G+D +  YRC ++ YQDTLYVHSNRQFF  C + GTVDFIFGNAA
Sbjct: 414 NWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAA 473

Query: 426 VVFQNSDLTPRKPGPKQQNMVTAQGRTDPNQNTGIVIQKCKIKATSDLEPVIKEFPSFLG 485
           VV QN  +  RKP   Q+N +TAQ R DPNQNTGI I   ++ A SDL+       ++LG
Sbjct: 474 VVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLG 533

Query: 486 RPWKDYARVVVMQTDITNVIDSKGWAPWGS--EIKKPYFAEYDNNGAGANTCCRVTW--- 545
           RPWK ++R V M + I   + ++GW  W +   +   Y+ EY N+G G+    RV+W   
Sbjct: 534 RPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGY 593

Query: 546 STIINAAQAKTFTAEPFIDGANWLPSTGFPYQLGL 569
             I + A+A  FT   FI G++WLPSTG  +  GL
Sbjct: 594 RVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011659194.19.2e-24277.57pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cu... [more]
TYK30782.11.6e-24177.66pectinesterase-like [Cucumis melo var. makuwa][more]
KAA0026177.11.9e-23976.92pectinesterase-like [Cucumis melo var. makuwa][more]
XP_008458040.22.5e-23976.24PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo][more]
XP_004139620.28.3e-23575.68pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cu... [more]
Match NameE-valueIdentityDescription
Q431439.4e-16554.70Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
O048868.8e-16353.89Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
P839488.8e-16354.06Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
O490062.5e-15752.98Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425342.2e-15051.88Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K6S64.4e-24277.57Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1[more]
A0A5D3E4E87.6e-24277.66Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G00133... [more]
A0A5A7SNA29.2e-24076.92Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370... [more]
A0A1S3C8611.2e-23976.24Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1[more]
A0A0A0K9Y34.0e-23575.68Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.11.8e-15852.98pectin methylesterase 3 [more]
AT1G53830.11.6e-15151.88pectin methylesterase 2 [more]
AT1G11580.19.8e-10141.62methylesterase PCR A [more]
AT2G45220.16.4e-10040.46Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G49220.11.8e-9437.39Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 34..190
e-value: 1.6E-30
score: 117.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 38..190
e-value: 1.6E-24
score: 87.0
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 11..195
e-value: 3.9E-23
score: 79.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 256..568
e-value: 3.4E-117
score: 393.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 33..196
e-value: 2.9E-29
score: 104.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 37..195
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 262..553
e-value: 1.1E-128
score: 428.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 426..446
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 7..567
NoneNo IPR availablePANTHERPTHR31707:SF180PECTINESTERASEcoord: 7..567
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 42..195
e-value: 3.47283E-33
score: 122.162
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 287..306
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 406..415
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 259..567

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc09G09140.1Clc09G09140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity