Clc09G08840 (gene) Watermelon (cordophanus) v2

Overview
NameClc09G08840
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionAsparagine--tRNA ligase
LocationClcChr09: 7502842 .. 7507579 (-)
RNA-Seq ExpressionClc09G08840
SyntenyClc09G08840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAGATGAACAAACTCTCCAAGAAAATCCAAACCTCAAAGGTATACCAAATCAAACTCTCCAAATCTCTCTCAGTTCATACCACAAAACTCCCCAAATCATTTCTCTGAAACAATCCTTTTCCCTCCATTAACAACCTCTCTTGATTTCCCCCTCTTCTTCCCCAAGAAAAGCATCTCTATCTTCTTCTATATCTCTCTATCCATGTCTCTTCTCCCTCATCATTCAATCCCCACATTTCTCTCCTCTTTCTTCCTCCACACTCTCCAATGGCCGCCCAACACCCTACAGTCTTCACCCCCGTCACCTTCTCCAAATACTCCAACCGGGTTCTTCTCAAAACTCTCTTCGATACCCACGATGGGGGTCTTGCGTTTGTGGGTCAGAAATTGGTGATCGGAGGATGGGTCAAGTCCTCCAAGGAAGTTATCAGAGAGGCTGCGCCGCTGCCGGTCGATGCCAATGTTAGGGCGGCAGCTGATGCCCTGCCTTCTAAGGCTAAGGATGTGAGCTGCATTGAGCTTATTCAGTCGCGGATTCCGGTTTTTAGGTCTTTGCTTAGGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAGTTGGAGACGTTTGCTCATAAGCCGCCTCGTCCTTCCACTGCTTTTCTGGCCGTTAATGATGGTTCTTGTGTTGCAAGCCTTCAGGTACCTCTTATTCATGCATGCCAACTGTTTGTTATTTTGTTATGCGTGTAGGACATGGCTAACTTGATGTTAGATGCATTTCACTATCAAAATTTATTATTTGTCTCTAATAGTACAAATTTTGTCTTTTTATTGAGAAATTATTCCAGTGATGCTGTTACAATATAAGTGATAGTTTTCCACCGTTGTATTTACGACAGTGAAGTATCTTGATTTATATCCAATGGTTATTAAAGGAACACAAATTTTGAATTTGGATTCTTACAAGGCAGCTATATTATTGAATAATTTGTCCTTTTTATGATTAACAAGTGATGTAATAGGATTAGGATGAATTAAGGTATCTTGTTTAAATCTTTGATTGATTAAGATTATGATCAATTTTCTTGATTAATAAGGATTAGAATTAGTTTTCTTGGTTAATTAGGATATAATTAGTTTGTTTTTCATTTCTCCATAATTAGAGAATTGTCTTCTGTTTTTATCCTTTAGGCTACATCAACAGGAGTTTGATTTTTTTCCCAATAATTGTTTGTTGTGTGTATTTCAAATCTAACTACTCATATCTATTGTGCTAACAAGTGTTCGACATGTGTAAGACATGGATAAAGTCCCCAAACAAAAGTGTTGTGATCATAGAAGATAATGAAATCCCTTGTTGACCATTTTTATTATTTGCATTCATACATCTGCCCTGGAGATTTTAAGAATTTATTGTGTCGCATGGGGATGTATTTGTATATTCTCCCCCAACCCACAAGAAAAGAGAAAGAAACAGAATTTAAAGTTGATGCAGAGTTAAGGAAGGTCTAATATTTTTTATTACAACGTTTTAGAGGATAATTGGTTGCTTAAGCTTTGGTTGTGCTTGATTTTTTGCATTCTCACAGTAATAAGAAACTTGTAGGTTGTTGTTGAGTCCTCGTTACATCATCCTACACATCTGATGCCAACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGACCCCAGTACCTGGGAAACATGTTGTTCAGCTTGAAGTCGAGAAAATCCTTCATTTAGGGAAGGTTGAACAGGAAAAGTATCCATTATCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAATATGCACAAGCTTCAACTATACATTCCATTTATTTCATATTTTATAGTTCTGTTTGCATGCCTATTCTTAAAGTGCCTATTAGTTTAAAGATCGTGCTTAATCAACAGCAGCACGTGAATTAGAACAAAGGCTTCTATTCATATTTTATATCTGTGATTGGTAAAGTAGACCTTGAGATCAGAAAATTGGTGATTTATACAGTTCAGTTTCTACTAAAAGAAAAGATTATATATATAGTTCAACATTCCTGAGCATGGAAAAGTGAAGTAAAGACTTTCACTTCATTGTAGATCTCTTTGGTAGGATCACTTGCATTGACCTTTATAGCACCTGCAAAGCTTGAGAAGAGAGTTTTGCATATTTATTTATTTAATCGTTCGAACTAATGAAATGTTGGATCTTGATCTTCATTGGTATTCTTTACTTTATGCAGGTAGCATCTGTTATGAGAATCCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAATCACAGCTTTCTTTACGTTCAATTGCCCAGCATCACGACCACAGACAGTGAGGGATTCAGCGAAAAGTTCCATGTTACAACACTTGGAGGTAAAGGTAAGAAAGTAGAACCAACAGAAGACAAAGAACCCGATGGTGTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTCGTCAAAGAGCTCGAGAGAAGCGAAAGCAACAAAGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAGGCCAGAGAAAAATCAAAATCCAAATCGAGGAAAGATAAAGTTAACCTTCCTGGAAATTTCTTCCCACAAGAAACTTATCTAACGGTTTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGCAATGTTTACTCCTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGGACTCGAGGTATGGATTTGATCTTTTACTGATTCCTTTGGATTGCTTAGAATTTGACTTCTAAATTATGGGTTTAAGATTCCATGTTGATTTAGCCATTAAAATCTTAATGTTATGTTCTGTGGTTAGTAGTTGATTATAATAACTTTGCCCCATTGAAATGAAGAATTTCCATCTTTTAACTTGCCTTTTGTATCATCATTTGGTGTCCAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAGCACTGTCTGGATGATATGAAATTTGTTCAAAAACGAATTGACAAAGCTAGCATTCATCGCCTTGAATCAGTTATATCATGTTCATTTGAGAAGGTCTCTTACACTGCGGCAATAGAAATCCTAGAAAAGGTGAAATCCAGATTATCTACTCTTCTATCATGTGACTCAGTTTTTCATTTCTATCCCTGCCCATCTGAATATTGAAAAAAAAAAAAAATCTTCACTGTCAGGGAACCGATCGAAAATCTCAACACTTGAAAAAGCTTAAATTTGGTGCTGCTTTAACTGTTGATCATCTCAGGTGTGCAAAATGTGCTGTTACAGTCAATAAGCAAAATGAGTTTTTTATTTTTCTTTCTTTTTTTTTTTTTAATGTATTTATTTACAAAAAGTTATTTCCTCTTCTTACATGAAACAGTTATCTGGCTGATGCCATCTTTAAAAAGCCTGTGATAGTCTATAACTACCCAAAAGAAACCAAGCCTTTTAATGTGCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGTTAGCACATGGGCCTACATTGACTCTTGGGAAAATTTATTTTTGGATGGAGCCTGTATTGAATGAAAATTGATTTCTGAAACTGGGACCCAACTGTTTGTAGGGTGGAGTAATAATTTCTGGCAGCCAAAAGGAGGAGCGTTTTGATATATTGATGACAAGGTATGATATTTTAGGGGTTTGAATATTATTTTGGTGTATGGACTTTCTGATCTATTCCATTCCAGTCTTCAAACTTCCAAATCTTCTATAATTGTTATTTAATAAATTATTCAACAGACTTAGACATTGTCTGTCACATCTGTTATTTATTTACTAGATCAATAGATATCTATTATCTACATAGGTTAGATGTTAGGAGGATCCACTATAGATTTTGGTTCTAATATAAATGGAAAGGTCTAAGATCAAAAAGGAAGATGGATGATTTTAAAGTTGAGGGATCAAAATCAAAAGAAGTTTAACTATCATGAGTTCAAACCGAGTGATTGCTGCTTCAGATAAAAACTTCACTAGTTTCACGACACTCCAATGTTTTCAAAACGCTCACTGTTAATAAAAAGAAAAATGTGAAAGTTATTGATCTTTGTTTGAATATGGATGGCTGTTGGCAGGATGAAGGAGATGGATCTGCCAAGGGAGCAATACGAGTGGTACTTGGAGATTCAAAGGCATGGAACAGTGAAGCATTCTGGTTTTAGTTTGGCATTTGACCGTTTCGTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCAGCAATTAAGCAGAATTCAAGGATAAAATTGTTCAAAAATGTTTTACGACGCAGCTGGCCCCGACCAATGGTGACGCTTAGAAAATGGAACTGAAAACTGTGTTTGTTTGTGTAAAAAGCCAAGGAAAAATGACTTCTATTGTTAGTGGATTTGTGTTTGTTTGTTGCTAAGATTTGTTCTTGTAAATATAAGAGAATTTGAAATCAAATGCATTATTTCTGGAGAAAGTGGATTCGAACTTACGACCTTGAGGTCGTAAATATAGTGTCTTAACTGCTATGCTTAGCTTCATTCCC

mRNA sequence

TGAGATGAACAAACTCTCCAAGAAAATCCAAACCTCAAAGGTATACCAAATCAAACTCTCCAAATCTCTCTCAGTTCATACCACAAAACTCCCCAAATCATTTCTCTGAAACAATCCTTTTCCCTCCATTAACAACCTCTCTTGATTTCCCCCTCTTCTTCCCCAAGAAAAGCATCTCTATCTTCTTCTATATCTCTCTATCCATGTCTCTTCTCCCTCATCATTCAATCCCCACATTTCTCTCCTCTTTCTTCCTCCACACTCTCCAATGGCCGCCCAACACCCTACAGTCTTCACCCCCGTCACCTTCTCCAAATACTCCAACCGGGTTCTTCTCAAAACTCTCTTCGATACCCACGATGGGGGTCTTGCGTTTGTGGGTCAGAAATTGGTGATCGGAGGATGGGTCAAGTCCTCCAAGGAAGTTATCAGAGAGGCTGCGCCGCTGCCGGTCGATGCCAATGTTAGGGCGGCAGCTGATGCCCTGCCTTCTAAGGCTAAGGATGTGAGCTGCATTGAGCTTATTCAGTCGCGGATTCCGGTTTTTAGGTCTTTGCTTAGGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAGTTGGAGACGTTTGCTCATAAGCCGCCTCGTCCTTCCACTGCTTTTCTGGCCGTTAATGATGGTTCTTGTGTTGCAAGCCTTCAGGTTGTTGTTGAGTCCTCGTTACATCATCCTACACATCTGATGCCAACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGACCCCAGTACCTGGGAAACATGTTGTTCAGCTTGAAGTCGAGAAAATCCTTCATTTAGGGAAGGTTGAACAGGAAAAGTATCCATTATCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAGCATCTGTTATGAGAATCCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAATCACAGCTTTCTTTACGTTCAATTGCCCAGCATCACGACCACAGACAGTGAGGGATTCAGCGAAAAGTTCCATGTTACAACACTTGGAGGTAAAGGTAAGAAAGTAGAACCAACAGAAGACAAAGAACCCGATGGTGTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTCGTCAAAGAGCTCGAGAGAAGCGAAAGCAACAAAGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAGGCCAGAGAAAAATCAAAATCCAAATCGAGGAAAGATAAAGTTAACCTTCCTGGAAATTTCTTCCCACAAGAAACTTATCTAACGGTTTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGCAATGTTTACTCCTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGGACTCGAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAGCACTGTCTGGATGATATGAAATTTGTTCAAAAACGAATTGACAAAGCTAGCATTCATCGCCTTGAATCAGTTATATCATGTTCATTTGAGAAGGTCTCTTACACTGCGGCAATAGAAATCCTAGAAAAGGTGAAATCCAGATTATCTACTCTTCTATCATGTGACTCAGGAACCGATCGAAAATCTCAACACTTGAAAAAGCTTAAATTTGGTGCTGCTTTAACTGTTGATCATCTCAGGTGTGCAAAATGTGCTGTTACAGTCAATAAGCAAAATGATTATCTGGCTGATGCCATCTTTAAAAAGCCTGTGATAGTCTATAACTACCCAAAAGAAACCAAGCCTTTTAATGTGCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTAATAATTTCTGGCAGCCAAAAGGAGGAGCGTTTTGATATATTGATGACAAGGATGAAGGAGATGGATCTGCCAAGGGAGCAATACGAGTGGTACTTGGAGATTCAAAGGCATGGAACAGTGAAGCATTCTGGTTTTAGTTTGGCATTTGACCGTTTCGTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCAGCAATTAAGCAGAATTCAAGGATAAAATTGTTCAAAAATGTTTTACGACGCAGCTGGCCCCGACCAATGGTGACGCTTAGAAAATGGAACTGAAAACTGTGTTTGTTTGTGTAAAAAGCCAAGGAAAAATGACTTCTATTGTTAGTGGATTTGTGTTTGTTTGTTGCTAAGATTTGTTCTTGTAAATATAAGAGAATTTGAAATCAAATGCATTATTTCTGGAGAAAGTGGATTCGAACTTACGACCTTGAGGTCGTAAATATAGTGTCTTAACTGCTATGCTTAGCTTCATTCCC

Coding sequence (CDS)

ATGGCCGCCCAACACCCTACAGTCTTCACCCCCGTCACCTTCTCCAAATACTCCAACCGGGTTCTTCTCAAAACTCTCTTCGATACCCACGATGGGGGTCTTGCGTTTGTGGGTCAGAAATTGGTGATCGGAGGATGGGTCAAGTCCTCCAAGGAAGTTATCAGAGAGGCTGCGCCGCTGCCGGTCGATGCCAATGTTAGGGCGGCAGCTGATGCCCTGCCTTCTAAGGCTAAGGATGTGAGCTGCATTGAGCTTATTCAGTCGCGGATTCCGGTTTTTAGGTCTTTGCTTAGGATGCTGTATGGAAATAATTTACACGGCGGTGAGAAGTTGGAGACGTTTGCTCATAAGCCGCCTCGTCCTTCCACTGCTTTTCTGGCCGTTAATGATGGTTCTTGTGTTGCAAGCCTTCAGGTTGTTGTTGAGTCCTCGTTACATCATCCTACACATCTGATGCCAACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGACCCCAGTACCTGGGAAACATGTTGTTCAGCTTGAAGTCGAGAAAATCCTTCATTTAGGGAAGGTTGAACAGGAAAAGTATCCATTATCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAGCATCTGTTATGAGAATCCGTAATGCCTTGACTTTTGCAACTCACACGTTCTTCCAAAATCACAGCTTTCTTTACGTTCAATTGCCCAGCATCACGACCACAGACAGTGAGGGATTCAGCGAAAAGTTCCATGTTACAACACTTGGAGGTAAAGGTAAGAAAGTAGAACCAACAGAAGACAAAGAACCCGATGGTGTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTCGTCAAAGAGCTCGAGAGAAGCGAAAGCAACAAAGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAGGCCAGAGAAAAATCAAAATCCAAATCGAGGAAAGATAAAGTTAACCTTCCTGGAAATTTCTTCCCACAAGAAACTTATCTAACGGTTTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGCAATGTTTACTCCTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGGACTCGAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAGCACTGTCTGGATGATATGAAATTTGTTCAAAAACGAATTGACAAAGCTAGCATTCATCGCCTTGAATCAGTTATATCATGTTCATTTGAGAAGGTCTCTTACACTGCGGCAATAGAAATCCTAGAAAAGGTGAAATCCAGATTATCTACTCTTCTATCATGTGACTCAGGAACCGATCGAAAATCTCAACACTTGAAAAAGCTTAAATTTGGTGCTGCTTTAACTGTTGATCATCTCAGGTGTGCAAAATGTGCTGTTACAGTCAATAAGCAAAATGATTATCTGGCTGATGCCATCTTTAAAAAGCCTGTGATAGTCTATAACTACCCAAAAGAAACCAAGCCTTTTAATGTGCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTAATAATTTCTGGCAGCCAAAAGGAGGAGCGTTTTGATATATTGATGACAAGGATGAAGGAGATGGATCTGCCAAGGGAGCAATACGAGTGGTACTTGGAGATTCAAAGGCATGGAACAGTGAAGCATTCTGGTTTTAGTTTGGCATTTGACCGTTTCGTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCAGCAATTAA

Protein sequence

MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPLPVDANVRAAADALPSKAKDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPPRPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEVEKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHSFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNLVKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDLVKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLSTLLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN
Homology
BLAST of Clc09G08840 vs. NCBI nr
Match: XP_038899280.1 (asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 577/645 (89.46%), Postives = 596/645 (92.40%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAA+HPTVFTPVTFSKYSNRVLLKTL DT DGGLAFVGQKLVIGGWVKSSKEVIREAAP 
Sbjct: 1   MAAEHPTVFTPVTFSKYSNRVLLKTLLDTADGGLAFVGQKLVIGGWVKSSKEVIREAAPS 60

Query: 61  PVDANVRAAADALPSKAKDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPPR 120
           P DA+VRA A+ALP KAKDVSCIE+IQSRIP+ RSLL+MLYGNNLHGGEKLET   KPP 
Sbjct: 61  PADASVRAVANALPPKAKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLETAVPKPPL 120

Query: 121 PSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEVE 180
           PSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILK+ PVPGKHVV+LEVE
Sbjct: 121 PSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKQVPVPGKHVVRLEVE 180

Query: 181 KILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHS 240
           KILH+GKVEQ+KYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRI NALTFATHTFFQNHS
Sbjct: 181 KILHVGKVEQQKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNHS 240

Query: 241 FLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNLV 300
           FLYVQLP+ITTTDSEGFSEKFHVTTL GKGKKVEPTEDKE DGVSLETVKAAITEKSNLV
Sbjct: 241 FLYVQLPTITTTDSEGFSEKFHVTTLAGKGKKVEPTEDKEHDGVSLETVKAAITEKSNLV 300

Query: 301 KELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLTV 360
           KELERSESNKEALVAAVQDLKKTNELALQLEA+EKSK+KSRKDKV +PGNFFPQETYLT 
Sbjct: 301 KELERSESNKEALVAAVQDLKKTNELALQLEAKEKSKTKSRKDKV-IPGNFFPQETYLTA 360

Query: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDLV 420
           SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA LE+AMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELENAMNCADDLV 420

Query: 421 KFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLSTL 480
           KFLSKWVL+HCLDDMKFVQKRIDK SIHRLESVISCSFEKVSY AAIEILEK        
Sbjct: 421 KFLSKWVLDHCLDDMKFVQKRIDKTSIHRLESVISCSFEKVSYIAAIEILEK-------- 480

Query: 481 LSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYPK 540
                 TD+KSQHLKKLKFGAALTVDHL              YLADAIFKKPVIVYNYPK
Sbjct: 481 -----ATDQKSQHLKKLKFGAALTVDHL-------------SYLADAIFKKPVIVYNYPK 540

Query: 541 ETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWYL 600
           ETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEERFD+LMTRMKEM LPREQYEWYL
Sbjct: 541 ETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERFDVLMTRMKEMGLPREQYEWYL 600

Query: 601 EIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           EIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN
Sbjct: 601 EIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 618

BLAST of Clc09G08840 vs. NCBI nr
Match: TYJ96325.1 (asparagine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 556/646 (86.07%), Postives = 586/646 (90.71%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAAQ PTVF+P+TFSKYSNRVLLKTL DT DGGL+FVGQKLV+GGWVKSSKEVIR+A P 
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PVDANVRAAADALPSKA-KDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPP 120
           P  A   A AD+LP K  KDVSCIE+IQSRIP+ RSLL+MLYGNNLHGGEKL+T  HKPP
Sbjct: 61  PAPA--PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120

Query: 121 RPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEV 180
            PSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LK+ PVPGKHVV+LEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILH+GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 300
           SFLYVQLP+ITTTDSEGFSEKFHVTTL GKGK+VE TEDKEPDG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNL 300

Query: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 360
           VKELERSESN+EALVAAVQDLKKTN+LALQLEAREKSKSKSRKDKV +PGNF PQETYLT
Sbjct: 301 VKELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKV-IPGNFLPQETYLT 360

Query: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 420
           VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA L+DAMNCADDL
Sbjct: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDL 420

Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 480
           VKFLSKWVLEHC+DDMKFVQKRIDK SIHRLES+ISCSFEKVSYTAAIEILEK       
Sbjct: 421 VKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEK------- 480

Query: 481 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 540
                  TD+KSQHLKKLKFGAALT DHL              YLAD IFKKPVIVYNYP
Sbjct: 481 ------ATDQKSQHLKKLKFGAALTADHL-------------SYLADTIFKKPVIVYNYP 540

Query: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 600
           KETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEER D+LMTRMKEM LPREQYEWY
Sbjct: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWY 600

Query: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV N
Sbjct: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVIN 617

BLAST of Clc09G08840 vs. NCBI nr
Match: XP_008458080.1 (PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo])

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 556/646 (86.07%), Postives = 585/646 (90.56%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAAQ PTVF+P+TFSKYSNRVLLKTL DT DGGL+FVGQKLV+GGWVKSSKEVIR+A P 
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PVDANVRAAADALPSKA-KDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPP 120
           P      A AD+LP K  KDVSCIE+IQSRIP+ RSLL+MLYGNNLHGGEKL+T  HKPP
Sbjct: 61  P------APADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120

Query: 121 RPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEV 180
            PSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LK+ PVPGKHVV+LEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILH+GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 300
           SFLYVQLP+ITTTDSEGFSEKFHVTTL GKGK+VE TEDKEPDG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNL 300

Query: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 360
           VKELERSESN+EALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV +PGNF PQETYLT
Sbjct: 301 VKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFLPQETYLT 360

Query: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 420
           VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA L+DAMNCADDL
Sbjct: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDL 420

Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 480
           VKFLSKWVLEHC+DDMKFVQKRIDK SIHRLES+ISCSFEKVSYTAAIEILEK       
Sbjct: 421 VKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEK------- 480

Query: 481 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 540
                  TD+KSQHLKKLKFGAALT DHL              YLAD IFKKPVIVYNYP
Sbjct: 481 ------ATDQKSQHLKKLKFGAALTADHL-------------SYLADTIFKKPVIVYNYP 540

Query: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 600
           KETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEER D+LMTRMKEM LPREQYEWY
Sbjct: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWY 600

Query: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV N
Sbjct: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVIN 613

BLAST of Clc09G08840 vs. NCBI nr
Match: XP_004149331.1 (asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 1070.1 bits (2766), Expect = 7.4e-309
Identity = 551/646 (85.29%), Postives = 581/646 (89.94%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAAQ PTVFTP+TFSKYSNRVLLKTL DT DGGL+FVGQK V+GGWVKSSKEVIR+A P 
Sbjct: 1   MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60

Query: 61  PVDANVRAAADALPSKA-KDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPP 120
           P      A AD+LP K  KDVSCIE+IQSRIP+ RSLL+MLYGN+LH GEKLET  HKPP
Sbjct: 61  P------APADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPP 120

Query: 121 RPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEV 180
            PSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LK+ PVPGKHVV+LEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILH+GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 300
           SFLYVQLP+ITTTDSEGFSEKFHVTTL GKGK+VEPTEDKEPDG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNL 300

Query: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 360
           VKELERSESN+EALV AVQDLKKTNELALQLEAREKSKSKSRKDKV +PGNFFPQETYLT
Sbjct: 301 VKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFFPQETYLT 360

Query: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 420
           VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA L+DAMNCADDL
Sbjct: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDL 420

Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 480
           VKFLSKWVLEHC+DDMKFVQKRIDK SIHRLES+ISCSFEKVSYT AIEILEK       
Sbjct: 421 VKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEK------- 480

Query: 481 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 540
                  T++KS HLKKLKFGAALT DHL              YLAD IFKKPVIV NYP
Sbjct: 481 ------ATNQKSPHLKKLKFGAALTDDHL-------------SYLADTIFKKPVIVCNYP 540

Query: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 600
           KETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEER D+LMTRM+EM LPREQYEWY
Sbjct: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWY 600

Query: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           LEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN
Sbjct: 601 LEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 613

BLAST of Clc09G08840 vs. NCBI nr
Match: KAA0026087.1 (asparagine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.3e-308
Identity = 552/646 (85.45%), Postives = 582/646 (90.09%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAAQ PTVF+P+TFSKYSNRVLLKTL DT DGGL+FVGQKLV+GGWVKSSKEVIR+A P 
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PVDANVRAAADALPSKA-KDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPP 120
           P  A   A AD+LP K  KDVSCIE+IQSRIP+ RSLL+MLYGNNLHGGEKL+T  HKPP
Sbjct: 61  PAPA--PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120

Query: 121 RPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEV 180
            PSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LK+ PVPGKHVV+LEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILH+GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 300
           SFLYVQLP+ITTTDSEGFSEKFHVTTL GKGK+VE TEDKEPDG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNL 300

Query: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 360
           VKELERSESN+EALVAAVQDLKKTN+LALQLEAREKSKSKSRKDKV +PGNF PQETYLT
Sbjct: 301 VKELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKV-IPGNFLPQETYLT 360

Query: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 420
           VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA L+DAMNCADDL
Sbjct: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDL 420

Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 480
           VKFLSKWVLEHC+DDMKFVQKRIDK SIHRLES+ISCSFEKVSYTAAIEILEKVKSRLST
Sbjct: 421 VKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKVKSRLST 480

Query: 481 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 540
           LLSCDS             F    T+               N YLAD IFKKPVIVYNYP
Sbjct: 481 LLSCDS-------------FFLLCTL---------------NSYLADTIFKKPVIVYNYP 540

Query: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 600
           KETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEER D+LMTRMKEM LPREQYEWY
Sbjct: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWY 600

Query: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV N
Sbjct: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVIN 615

BLAST of Clc09G08840 vs. ExPASy Swiss-Prot
Match: Q9SW95 (Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 PE=1 SV=2)

HSP 1 Score: 570.9 bits (1470), Expect = 1.9e-161
Identity = 321/654 (49.08%), Postives = 434/654 (66.36%), Query Frame = 0

Query: 11  PVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPLPVDANVRAAA 70
           P+T SKYS RV LKTL D  D G    G+++VIGGWVKS++ V + + P P+      + 
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  DALPSKA---KDVSCIELIQSRIPVFRSLLRMLYGNNLHGG------EKLETFAHK--PP 130
            +   +A    ++ C E+IQS++ +F+    +L G    GG      +K E    K  PP
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSG----GGKTYPIFDKTELAGQKAVPP 139

Query: 131 RPSTAFLAVNDGSCVASLQVVVESSLH--HPTHLMPTGTCILVEGILKRTPV--PGKHVV 190
                +  ++DGS ++SLQVVV+S+L     T LM  GTCI+ EG+L R P+    KHV+
Sbjct: 140 PEYVFYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVL-RLPLAASAKHVI 199

Query: 191 QLEVEKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTF 250
           +LE EK+LH+G V+ EKYPLS+K+LP+  LR FS+FRPRTTTV SV R+ +ALT A+HTF
Sbjct: 200 ELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTF 259

Query: 251 FQNHSFLYVQLPSITTTDSEGFSEKFHVTTLGGK--GKKVEPTEDKEPDGVSLETVKAAI 310
            Q H F YVQ+P ITTT   GF E F VTTL GK   K+ +    +E DG S++TVKA I
Sbjct: 260 LQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVI 319

Query: 311 TEKSNLVKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKS--RKDKVNLPGNF 370
            EK+ L+  L+RS+SN+E +VAAV DLKKTN+LA Q+E ++KSK+ +  + +K++   +F
Sbjct: 320 KEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDF 379

Query: 371 FPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLED 430
           F ++TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFA L+D
Sbjct: 380 FGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDD 439

Query: 431 AMNCADDLVKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILE 490
           AM+CAD+  KFL K+VLE+  +DMKF+ KR+DK    RLE+  S S  + SYT  I +L+
Sbjct: 440 AMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQ 499

Query: 491 KVKSRLSTLLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKK 550
           K  +               ++   K ++G ALT +HL              YL D I+K 
Sbjct: 500 KATT---------------TKFETKPEWGVALTTEHL-------------SYLTDEIYKG 559

Query: 551 PVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDL 610
           PVIV+ YPK  K F VRLNDD KTVAAFD+V+PK GV+I+GSQ EERF+IL  R+ E   
Sbjct: 560 PVIVHTYPKAIKQFYVRLNDDKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGF 619

Query: 611 PREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
            RE++EWYL+++RHGTVKHSG SL+ ++ +L+ TGL DIKD IPFPRS+GK +N
Sbjct: 620 TREKFEWYLDLRRHGTVKHSGISLSMEQMLLYATGLPDIKDAIPFPRSWGKANN 638

BLAST of Clc09G08840 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 4.7e-128
Identity = 260/635 (40.94%), Postives = 373/635 (58.74%), Query Frame = 0

Query: 15  SKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPLPVDANVRAAADALP 74
           +++SNRVL++T+ D  DGG    GQ + IGGWVKS ++                      
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD---------------------- 86

Query: 75  SKAKDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPPRPSTAFLAVNDGSCV 134
                                             +   TF         +FLAVNDGSC 
Sbjct: 87  ----------------------------------QGKRTF---------SFLAVNDGSCP 146

Query: 135 ASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGK---HVVQLEVEKILHLGKVEQE 194
           A+LQV+V+ SL+  ++L+ TGTC+ V+G+LK  P  GK     ++L V K++ +G V+  
Sbjct: 147 ANLQVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDAS 206

Query: 195 KYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHSFLYVQLPSITT 254
           KYPL + KL ++ LR   + R RT ++++V RIRNAL FATH+FFQ HSFLY+  P ITT
Sbjct: 207 KYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITT 266

Query: 255 TDSEGFSEKFHVTTLGGKGKKVEP--TEDKEPDGVSLETVKAAITEKSNLVKELERSESN 314
           +D EG  E F  TTL    +++E    ++  P    +E  +  + E+ N+V EL+ ++++
Sbjct: 267 SDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKAS 326

Query: 315 KEALVAAVQDLKKTNELALQLEAREKSK-SKSRKD-KVNLPGNFFPQETYLTVSGQLHLE 374
           KEA+ AAV +LK   E    ++ R + +    +KD  ++   +FF ++ +LTVSGQL +E
Sbjct: 327 KEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVE 386

Query: 375 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDLVKFLSKWV 434
           TYACAL NVY+ GP FRA+   +++H AE WMVE EIAFA LED MNCA+  VK++  W+
Sbjct: 387 TYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWL 446

Query: 435 LEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLSTLLSCDSGT 494
           LE C  DM+ + K  D   I RL+ V S  F +++YT AIE+LE+  +            
Sbjct: 447 LEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVA------------ 506

Query: 495 DRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYPKETKPFNV 554
            +  +    +++G  L  +H R             YL + +F+KP+IVYNYPK  K F +
Sbjct: 507 -KGKEFDNNVEWGIDLASEHER-------------YLTEVLFQKPLIVYNYPKGIKAFYM 566

Query: 555 RLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWYLEIQRHGT 614
           RLNDD KTVAA DV++PK G +I GSQ+EER+D++  R++EM LP E YEWYL+++R+GT
Sbjct: 567 RLNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGT 569

Query: 615 VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGK 643
           VKH GF L F+R +LF TGL +I+DVIPFPR  GK
Sbjct: 627 VKHCGFGLGFERMILFATGLDNIRDVIPFPRYPGK 569

BLAST of Clc09G08840 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.8e-119
Identity = 234/526 (44.49%), Postives = 333/526 (63.31%), Query Frame = 0

Query: 124 AFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPG--KHVVQLEVEK 183
           AFL VNDGSC A+LQV+V+SSL+  + L+ TGTC+ V+G+LK  P     K  ++L VE 
Sbjct: 68  AFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGKGLKQSIELSVET 127

Query: 184 ILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHSF 243
           ++ +G V+   YPL + KL  +FLR   + R RT  +++V RIRNAL FATH+FFQ HSF
Sbjct: 128 VIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSF 187

Query: 244 LYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEP--TEDKEPDGVSLETVKAAITEKSNL 303
           LY+  P ITT+D EG  E F VTTL    ++VE    ++  P    +E  +  + E+   
Sbjct: 188 LYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEA 247

Query: 304 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKD--KVNLPGNFFPQETY 363
           V +L+ ++++KE + A+V  L         +E R + K    K+  K++   +FF ++ +
Sbjct: 248 VAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAF 307

Query: 364 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCAD 423
           LTVSGQL +ETYACAL +VY+ GP FRA+   +++H AE WMVE EIAFA + D MNCA+
Sbjct: 308 LTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAE 367

Query: 424 DLVKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRL 483
             VK++ KW+++ C DDM+ + K +D+    RL  V   SF++V+YT AIE LEK  ++ 
Sbjct: 368 AYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQG 427

Query: 484 STLLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIF-KKPVIVY 543
             +               K+++G  L  +H R             YL +  F +KP+IVY
Sbjct: 428 KVVFD------------NKVEWGIDLASEHER-------------YLTEVEFDQKPIIVY 487

Query: 544 NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQY 603
           NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+EER+D++  R++EM LP E Y
Sbjct: 488 NYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPY 547

Query: 604 EWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGK 643
           EWYL+++R+GTVKH GF L F+R + F TG+ +I+DVIPFPR  GK
Sbjct: 548 EWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVIPFPRYPGK 568

BLAST of Clc09G08840 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.1e-92
Identity = 194/521 (37.24%), Postives = 288/521 (55.28%), Query Frame = 0

Query: 125 FLAVNDGSCVASLQVVVESSLHHPTHL---MPTGTCILVEGILKRTPVPGKHVVQLEVEK 184
           F+ +NDGS +++ Q++    +     L   + TG  +   G +  +P   ++ ++++   
Sbjct: 40  FIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVSVSAIGTIVESPGKEQN-LEMQATA 99

Query: 185 ILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHSF 244
           I  +GK + E Y L +K+   +FLR  ++ RPRT T+ +V R+RNAL FATH FFQ   F
Sbjct: 100 ITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGF 159

Query: 245 LYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNLVK 304
           LY+  P IT +D EG  + F VTTL                                   
Sbjct: 160 LYIHTPIITGSDCEGAGKMFQVTTLD---------------------------------- 219

Query: 305 ELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLTVS 364
                                           + + +++ + +V+   +FF + TYLTVS
Sbjct: 220 --------------------------------QNNPARTPEGRVDYTQDFFGKPTYLTVS 279

Query: 365 GQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDLVK 424
           GQL+ E YACAL +VY+ GP FRA+   +++H AE WM+E E+AFA L D M+CA+D +K
Sbjct: 280 GQLNGEIYACALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLK 339

Query: 425 FLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLSTLL 484
           ++ K+VL++C +DM+F  K +    I RLE VI+ SFE+ SYT A+ ILEK         
Sbjct: 340 YILKYVLDNCQEDMEFFNKHVATDLISRLEHVINTSFERASYTYAVRILEK--------- 399

Query: 485 SCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYPKE 544
                 D+K ++   +K+G  L  +H R             +LA+  F KPVI+ +YPK+
Sbjct: 400 -----ADKKFEY--PVKWGLDLQSEHER-------------FLAEEFFGKPVILTDYPKD 459

Query: 545 TKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWYLE 604
            K F +R N+D KTVAA DV++PK G II GSQ+EER  +L +++KE +LP E+Y WYLE
Sbjct: 460 IKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLESKLKEFNLPAEEYWWYLE 464

Query: 605 IQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGK 643
           +++ G+V HSGF   F+R V FTTG+ +I+DVIPFPR  GK
Sbjct: 520 LRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGK 464

BLAST of Clc09G08840 vs. ExPASy Swiss-Prot
Match: P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)

HSP 1 Score: 328.6 bits (841), Expect = 1.6e-88
Identity = 198/520 (38.08%), Postives = 281/520 (54.04%), Query Frame = 0

Query: 124 AFLAVNDGSCVASLQVVVESSLHHPTHL---MPTGTCILVEGILKRTPVPGK-HVVQLEV 183
           +F+ VNDGS +A+LQVV++ SL     L   + TG  ++VEG  K  P PGK   V+L+ 
Sbjct: 35  SFVEVNDGSSLANLQVVLDGSLADYDRLLSQLQTGAALVVEG--KLAPSPGKGQRVELKA 94

Query: 184 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 243
            K+  LG  +   YPL +K+   +FLR   + RPRT T+ +VMR+RNA   A H FFQ  
Sbjct: 95  TKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRVRNACATAIHQFFQER 154

Query: 244 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 303
            FL+V  P IT +D EG  + F VTTL                                 
Sbjct: 155 GFLWVHTPIITASDCEGAGDLFKVTTL--------------------------------- 214

Query: 304 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 363
             +L+R   N E                                 ++   +FF ++ YLT
Sbjct: 215 --DLQRVPKNGEG--------------------------------IDYSQDFFGKQAYLT 274

Query: 364 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 423
           VSGQL  E  A A  NVY+ GP FRA+   +++H AE WMVE E+AF  LE     A++ 
Sbjct: 275 VSGQLEAEVMALAFQNVYTFGPTFRAENSNTSRHLAEFWMVEPEMAFCDLEGDRQWAEEF 334

Query: 424 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 483
           +K++ K+VLE C +DM+F  +RID   +   +++I+  F  ++Y+ AI++LEK       
Sbjct: 335 LKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAWLTYSEAIKLLEK------- 394

Query: 484 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 543
                   D+K ++   + +G  L  +H R             YLA+ +FK+P IV +YP
Sbjct: 395 -------ADQKFEY--PVAWGVDLQSEHER-------------YLAETVFKRPTIVTDYP 454

Query: 544 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 603
           K+ K F +RLNDDGKTVAA D++ PK G II GSQ+EER DIL  RM+E  +P E   WY
Sbjct: 455 KDIKAFYMRLNDDGKTVAAMDILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWY 456

Query: 604 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRS 640
           L+++R+G+V H+GF L F+R V F TG+ +I+DVIPFPR+
Sbjct: 515 LDLRRYGSVPHAGFGLGFERIVQFMTGMANIRDVIPFPRT 456

BLAST of Clc09G08840 vs. ExPASy TrEMBL
Match: A0A5D3BD07 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00300 PE=3 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 556/646 (86.07%), Postives = 586/646 (90.71%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAAQ PTVF+P+TFSKYSNRVLLKTL DT DGGL+FVGQKLV+GGWVKSSKEVIR+A P 
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PVDANVRAAADALPSKA-KDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPP 120
           P  A   A AD+LP K  KDVSCIE+IQSRIP+ RSLL+MLYGNNLHGGEKL+T  HKPP
Sbjct: 61  PAPA--PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120

Query: 121 RPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEV 180
            PSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LK+ PVPGKHVV+LEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILH+GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 300
           SFLYVQLP+ITTTDSEGFSEKFHVTTL GKGK+VE TEDKEPDG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNL 300

Query: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 360
           VKELERSESN+EALVAAVQDLKKTN+LALQLEAREKSKSKSRKDKV +PGNF PQETYLT
Sbjct: 301 VKELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKV-IPGNFLPQETYLT 360

Query: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 420
           VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA L+DAMNCADDL
Sbjct: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDL 420

Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 480
           VKFLSKWVLEHC+DDMKFVQKRIDK SIHRLES+ISCSFEKVSYTAAIEILEK       
Sbjct: 421 VKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEK------- 480

Query: 481 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 540
                  TD+KSQHLKKLKFGAALT DHL              YLAD IFKKPVIVYNYP
Sbjct: 481 ------ATDQKSQHLKKLKFGAALTADHL-------------SYLADTIFKKPVIVYNYP 540

Query: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 600
           KETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEER D+LMTRMKEM LPREQYEWY
Sbjct: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWY 600

Query: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV N
Sbjct: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVIN 617

BLAST of Clc09G08840 vs. ExPASy TrEMBL
Match: A0A1S3C6Z5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1)

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 556/646 (86.07%), Postives = 585/646 (90.56%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAAQ PTVF+P+TFSKYSNRVLLKTL DT DGGL+FVGQKLV+GGWVKSSKEVIR+A P 
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PVDANVRAAADALPSKA-KDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPP 120
           P      A AD+LP K  KDVSCIE+IQSRIP+ RSLL+MLYGNNLHGGEKL+T  HKPP
Sbjct: 61  P------APADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120

Query: 121 RPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEV 180
            PSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LK+ PVPGKHVV+LEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILH+GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 300
           SFLYVQLP+ITTTDSEGFSEKFHVTTL GKGK+VE TEDKEPDG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNL 300

Query: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 360
           VKELERSESN+EALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV +PGNF PQETYLT
Sbjct: 301 VKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFLPQETYLT 360

Query: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 420
           VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA L+DAMNCADDL
Sbjct: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDL 420

Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 480
           VKFLSKWVLEHC+DDMKFVQKRIDK SIHRLES+ISCSFEKVSYTAAIEILEK       
Sbjct: 421 VKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEK------- 480

Query: 481 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 540
                  TD+KSQHLKKLKFGAALT DHL              YLAD IFKKPVIVYNYP
Sbjct: 481 ------ATDQKSQHLKKLKFGAALTADHL-------------SYLADTIFKKPVIVYNYP 540

Query: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 600
           KETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEER D+LMTRMKEM LPREQYEWY
Sbjct: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWY 600

Query: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV N
Sbjct: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVIN 613

BLAST of Clc09G08840 vs. ExPASy TrEMBL
Match: A0A0A0K8G1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.6e-309
Identity = 551/646 (85.29%), Postives = 581/646 (89.94%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAAQ PTVFTP+TFSKYSNRVLLKTL DT DGGL+FVGQK V+GGWVKSSKEVIR+A P 
Sbjct: 1   MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60

Query: 61  PVDANVRAAADALPSKA-KDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPP 120
           P      A AD+LP K  KDVSCIE+IQSRIP+ RSLL+MLYGN+LH GEKLET  HKPP
Sbjct: 61  P------APADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPP 120

Query: 121 RPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEV 180
            PSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LK+ PVPGKHVV+LEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILH+GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 300
           SFLYVQLP+ITTTDSEGFSEKFHVTTL GKGK+VEPTEDKEPDG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNL 300

Query: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 360
           VKELERSESN+EALV AVQDLKKTNELALQLEAREKSKSKSRKDKV +PGNFFPQETYLT
Sbjct: 301 VKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFFPQETYLT 360

Query: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 420
           VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA L+DAMNCADDL
Sbjct: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDL 420

Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 480
           VKFLSKWVLEHC+DDMKFVQKRIDK SIHRLES+ISCSFEKVSYT AIEILEK       
Sbjct: 421 VKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEK------- 480

Query: 481 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 540
                  T++KS HLKKLKFGAALT DHL              YLAD IFKKPVIV NYP
Sbjct: 481 ------ATNQKSPHLKKLKFGAALTDDHL-------------SYLADTIFKKPVIVCNYP 540

Query: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 600
           KETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEER D+LMTRM+EM LPREQYEWY
Sbjct: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWY 600

Query: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           LEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN
Sbjct: 601 LEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 613

BLAST of Clc09G08840 vs. ExPASy TrEMBL
Match: A0A5A7SMX6 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00190 PE=3 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 6.2e-309
Identity = 552/646 (85.45%), Postives = 582/646 (90.09%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAAQ PTVF+P+TFSKYSNRVLLKTL DT DGGL+FVGQKLV+GGWVKSSKEVIR+A P 
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PVDANVRAAADALPSKA-KDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPP 120
           P  A   A AD+LP K  KDVSCIE+IQSRIP+ RSLL+MLYGNNLHGGEKL+T  HKPP
Sbjct: 61  PAPA--PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120

Query: 121 RPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLEV 180
            PSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LK+ PVPGKHVV+LEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNH 240
           EKILH+GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASV RIR+ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 SFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKSNL 300
           SFLYVQLP+ITTTDSEGFSEKFHVTTL GKGK+VE TEDKEPDG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNL 300

Query: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETYLT 360
           VKELERSESN+EALVAAVQDLKKTN+LALQLEAREKSKSKSRKDKV +PGNF PQETYLT
Sbjct: 301 VKELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKV-IPGNFLPQETYLT 360

Query: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDL 420
           VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFA L+DAMNCADDL
Sbjct: 361 VSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDL 420

Query: 421 VKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLST 480
           VKFLSKWVLEHC+DDMKFVQKRIDK SIHRLES+ISCSFEKVSYTAAIEILEKVKSRLST
Sbjct: 421 VKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKVKSRLST 480

Query: 481 LLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYP 540
           LLSCDS             F    T+               N YLAD IFKKPVIVYNYP
Sbjct: 481 LLSCDS-------------FFLLCTL---------------NSYLADTIFKKPVIVYNYP 540

Query: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWY 600
           KETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEER D+LMTRMKEM LPREQYEWY
Sbjct: 541 KETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWY 600

Query: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV N
Sbjct: 601 LEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVIN 615

BLAST of Clc09G08840 vs. ExPASy TrEMBL
Match: A0A6J1KTV9 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497586 PE=4 SV=1)

HSP 1 Score: 1033.5 bits (2671), Expect = 3.8e-298
Identity = 533/649 (82.13%), Postives = 567/649 (87.37%), Query Frame = 0

Query: 1   MAAQHPTVFTPVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPL 60
           MAA+HPT FTPVTFSKYS RVLLKT+ D  DGGL FV QKLVIGGWVKSSKEV REAA +
Sbjct: 1   MAAEHPTAFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAA-V 60

Query: 61  PVDANVRAAADALPSKAKDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHK--P 120
           P DA+VR A DALP +AKDVSCIE++QSRIP+ RSLLRMLYGN+LH GEKLET  HK  P
Sbjct: 61  PADASVRTAGDALPRRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETPVHKPPP 120

Query: 121 PRPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGKHVVQLE 180
           P PST FL VNDGSCVASLQVVVESSLHHP  LMPTGTCILVEGILKR P PGKH+V+LE
Sbjct: 121 PLPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLE 180

Query: 181 VEKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQN 240
           VEKILH+GKVEQEKYPL+QKKLPMD LRKFSYFRPRTTTVASVMRI NALTFATHTFFQN
Sbjct: 181 VEKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQN 240

Query: 241 HSFLYVQLPSITTTDSEGFSEKFHVTTLGGKG--KKVEPTEDKEPDGVSLETVKAAITEK 300
           H FLYVQLP+ITTTDSEGFSEKFHVTT  GK   KK+EPTEDKE DGVSLETVKAA  EK
Sbjct: 241 HGFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEK 300

Query: 301 SNLVKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQET 360
            N+VKELERSESN+EAL AAVQDLKKTNELALQLEAR K KS+ +KDKV++  NFFPQET
Sbjct: 301 RNIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQET 360

Query: 361 YLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCA 420
           YLTVSGQLHLETYACALGNVYSCGPRFRAD+I SAKHAAEMWMVEVEIAFA LED +NCA
Sbjct: 361 YLTVSGQLHLETYACALGNVYSCGPRFRADRIVSAKHAAEMWMVEVEIAFAELEDVINCA 420

Query: 421 DDLVKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSR 480
           DDLVKFLSKWVLEHCLDDMKFVQKRIDK S+HRLES I+ SFEKVSYTAA+EIL+K    
Sbjct: 421 DDLVKFLSKWVLEHCLDDMKFVQKRIDKTSVHRLESTITSSFEKVSYTAAVEILDK---- 480

Query: 481 LSTLLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVY 540
                     TD+KSQ LK+LKFGAALT DHL              YLADAIFKKPVIVY
Sbjct: 481 ---------ATDQKSQRLKRLKFGAALTDDHL-------------SYLADAIFKKPVIVY 540

Query: 541 NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQY 600
           NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV+ISGSQKEERFD+L++RMKEM LPREQY
Sbjct: 541 NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERFDVLLSRMKEMGLPREQY 600

Query: 601 EWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
           EWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRS GKVS+
Sbjct: 601 EWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSLGKVSH 622

BLAST of Clc09G08840 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 570.9 bits (1470), Expect = 1.3e-162
Identity = 321/654 (49.08%), Postives = 434/654 (66.36%), Query Frame = 0

Query: 11  PVTFSKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPLPVDANVRAAA 70
           P+T SKYS RV LKTL D  D G    G+++VIGGWVKS++ V + + P P+      + 
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  DALPSKA---KDVSCIELIQSRIPVFRSLLRMLYGNNLHGG------EKLETFAHK--PP 130
            +   +A    ++ C E+IQS++ +F+    +L G    GG      +K E    K  PP
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSG----GGKTYPIFDKTELAGQKAVPP 139

Query: 131 RPSTAFLAVNDGSCVASLQVVVESSLH--HPTHLMPTGTCILVEGILKRTPV--PGKHVV 190
                +  ++DGS ++SLQVVV+S+L     T LM  GTCI+ EG+L R P+    KHV+
Sbjct: 140 PEYVFYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVL-RLPLAASAKHVI 199

Query: 191 QLEVEKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTF 250
           +LE EK+LH+G V+ EKYPLS+K+LP+  LR FS+FRPRTTTV SV R+ +ALT A+HTF
Sbjct: 200 ELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTF 259

Query: 251 FQNHSFLYVQLPSITTTDSEGFSEKFHVTTLGGK--GKKVEPTEDKEPDGVSLETVKAAI 310
            Q H F YVQ+P ITTT   GF E F VTTL GK   K+ +    +E DG S++TVKA I
Sbjct: 260 LQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVI 319

Query: 311 TEKSNLVKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKS--RKDKVNLPGNF 370
            EK+ L+  L+RS+SN+E +VAAV DLKKTN+LA Q+E ++KSK+ +  + +K++   +F
Sbjct: 320 KEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDF 379

Query: 371 FPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLED 430
           F ++TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFA L+D
Sbjct: 380 FGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDD 439

Query: 431 AMNCADDLVKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILE 490
           AM+CAD+  KFL K+VLE+  +DMKF+ KR+DK    RLE+  S S  + SYT  I +L+
Sbjct: 440 AMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQ 499

Query: 491 KVKSRLSTLLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKK 550
           K  +               ++   K ++G ALT +HL              YL D I+K 
Sbjct: 500 KATT---------------TKFETKPEWGVALTTEHL-------------SYLTDEIYKG 559

Query: 551 PVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDL 610
           PVIV+ YPK  K F VRLNDD KTVAAFD+V+PK GV+I+GSQ EERF+IL  R+ E   
Sbjct: 560 PVIVHTYPKAIKQFYVRLNDDKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGF 619

Query: 611 PREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 646
            RE++EWYL+++RHGTVKHSG SL+ ++ +L+ TGL DIKD IPFPRS+GK +N
Sbjct: 620 TREKFEWYLDLRRHGTVKHSGISLSMEQMLLYATGLPDIKDAIPFPRSWGKANN 638

BLAST of Clc09G08840 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 459.9 bits (1182), Expect = 3.3e-129
Identity = 260/635 (40.94%), Postives = 373/635 (58.74%), Query Frame = 0

Query: 15  SKYSNRVLLKTLFDTHDGGLAFVGQKLVIGGWVKSSKEVIREAAPLPVDANVRAAADALP 74
           +++SNRVL++T+ D  DGG    GQ + IGGWVKS ++                      
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD---------------------- 86

Query: 75  SKAKDVSCIELIQSRIPVFRSLLRMLYGNNLHGGEKLETFAHKPPRPSTAFLAVNDGSCV 134
                                             +   TF         +FLAVNDGSC 
Sbjct: 87  ----------------------------------QGKRTF---------SFLAVNDGSCP 146

Query: 135 ASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPGK---HVVQLEVEKILHLGKVEQE 194
           A+LQV+V+ SL+  ++L+ TGTC+ V+G+LK  P  GK     ++L V K++ +G V+  
Sbjct: 147 ANLQVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDAS 206

Query: 195 KYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHSFLYVQLPSITT 254
           KYPL + KL ++ LR   + R RT ++++V RIRNAL FATH+FFQ HSFLY+  P ITT
Sbjct: 207 KYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITT 266

Query: 255 TDSEGFSEKFHVTTLGGKGKKVEP--TEDKEPDGVSLETVKAAITEKSNLVKELERSESN 314
           +D EG  E F  TTL    +++E    ++  P    +E  +  + E+ N+V EL+ ++++
Sbjct: 267 SDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKAS 326

Query: 315 KEALVAAVQDLKKTNELALQLEAREKSK-SKSRKD-KVNLPGNFFPQETYLTVSGQLHLE 374
           KEA+ AAV +LK   E    ++ R + +    +KD  ++   +FF ++ +LTVSGQL +E
Sbjct: 327 KEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVE 386

Query: 375 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDLVKFLSKWV 434
           TYACAL NVY+ GP FRA+   +++H AE WMVE EIAFA LED MNCA+  VK++  W+
Sbjct: 387 TYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWL 446

Query: 435 LEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRLSTLLSCDSGT 494
           LE C  DM+ + K  D   I RL+ V S  F +++YT AIE+LE+  +            
Sbjct: 447 LEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVA------------ 506

Query: 495 DRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIFKKPVIVYNYPKETKPFNV 554
            +  +    +++G  L  +H R             YL + +F+KP+IVYNYPK  K F +
Sbjct: 507 -KGKEFDNNVEWGIDLASEHER-------------YLTEVLFQKPLIVYNYPKGIKAFYM 566

Query: 555 RLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQYEWYLEIQRHGT 614
           RLNDD KTVAA DV++PK G +I GSQ+EER+D++  R++EM LP E YEWYL+++R+GT
Sbjct: 567 RLNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGT 569

Query: 615 VKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGK 643
           VKH GF L F+R +LF TGL +I+DVIPFPR  GK
Sbjct: 627 VKHCGFGLGFERMILFATGLDNIRDVIPFPRYPGK 569

BLAST of Clc09G08840 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 431.4 bits (1108), Expect = 1.3e-120
Identity = 234/526 (44.49%), Postives = 333/526 (63.31%), Query Frame = 0

Query: 124 AFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKRTPVPG--KHVVQLEVEK 183
           AFL VNDGSC A+LQV+V+SSL+  + L+ TGTC+ V+G+LK  P     K  ++L VE 
Sbjct: 68  AFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGKGLKQSIELSVET 127

Query: 184 ILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQNHSF 243
           ++ +G V+   YPL + KL  +FLR   + R RT  +++V RIRNAL FATH+FFQ HSF
Sbjct: 128 VIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSF 187

Query: 244 LYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEP--TEDKEPDGVSLETVKAAITEKSNL 303
           LY+  P ITT+D EG  E F VTTL    ++VE    ++  P    +E  +  + E+   
Sbjct: 188 LYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEA 247

Query: 304 VKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKD--KVNLPGNFFPQETY 363
           V +L+ ++++KE + A+V  L         +E R + K    K+  K++   +FF ++ +
Sbjct: 248 VAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAF 307

Query: 364 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCAD 423
           LTVSGQL +ETYACAL +VY+ GP FRA+   +++H AE WMVE EIAFA + D MNCA+
Sbjct: 308 LTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAE 367

Query: 424 DLVKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRL 483
             VK++ KW+++ C DDM+ + K +D+    RL  V   SF++V+YT AIE LEK  ++ 
Sbjct: 368 AYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQG 427

Query: 484 STLLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIF-KKPVIVY 543
             +               K+++G  L  +H R             YL +  F +KP+IVY
Sbjct: 428 KVVFD------------NKVEWGIDLASEHER-------------YLTEVEFDQKPIIVY 487

Query: 544 NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQY 603
           NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+EER+D++  R++EM LP E Y
Sbjct: 488 NYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPY 547

Query: 604 EWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGK 643
           EWYL+++R+GTVKH GF L F+R + F TG+ +I+DVIPFPR  GK
Sbjct: 548 EWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVIPFPRYPGK 568

BLAST of Clc09G08840 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 2.3e-85
Identity = 188/523 (35.95%), Postives = 285/523 (54.49%), Query Frame = 0

Query: 122 STAFLAVNDGSCVASLQVVVESSLHHPTHL----MPTGTCILVEGILKRTPVPGKHVVQL 181
           S  F+ +NDGSC+++LQ V+ S       +    + TG  + V+G +  +    K  V+L
Sbjct: 126 SVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVASQ-GTKQKVEL 185

Query: 182 EVEKILHLGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIRNALTFATHTFFQ 241
           +VEKI+ +G+ +   YP+ +K++  +FLR  ++ RPRT T  +V R+RN L +ATH FFQ
Sbjct: 186 KVEKIIVVGECD-SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQ 245

Query: 242 NHSFLYVQLPSITTTDSEGFSEKFHVTTLGGKGKKVEPTEDKEPDGVSLETVKAAITEKS 301
              F++V  P IT +D EG  E+F VTTL                               
Sbjct: 246 ESGFVWVASPIITASDCEGAGEQFCVTTL------------------------------- 305

Query: 302 NLVKELERSESNKEALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVNLPGNFFPQETY 361
                +  S  N +  + A+                     K++   ++   +FF +  +
Sbjct: 306 -----IPSSHENTDTSIDAI--------------------PKTKGGLIDWSQDFFGKPAF 365

Query: 362 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCAD 421
           LTVSGQL+ ETYA AL +VY+ GP FRA+   +++H AE WM+E E+AFA L+D M CA 
Sbjct: 366 LTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACAT 425

Query: 422 DLVKFLSKWVLEHCLDDMKFVQKRIDKASIHRLESVISCSFEKVSYTAAIEILEKVKSRL 481
             ++++ K+VL++C +DM+F    I+K  I RL  V    F ++ YT AIEIL K   + 
Sbjct: 426 AYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKKF 485

Query: 482 STLLSCDSGTDRKSQHLKKLKFGAALTVDHLRCAKCAVTVNKQNDYLADAIF-KKPVIVY 541
                              +K+G  L  +H R             Y+ +  F  +PVI+ 
Sbjct: 486 DF----------------PVKWGLDLQSEHER-------------YITEEAFGGRPVIIR 545

Query: 542 NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVIISGSQKEERFDILMTRMKEMDLPREQY 601
           +YPKE K F +R NDDGKTVAA D+++P+ G +I GSQ+EER ++L  R+ E+ L +E Y
Sbjct: 546 DYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESY 561

Query: 602 EWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRS 640
            WYL+++R+G+V H+GF L F+R V F TG+ +I+DVIPFPR+
Sbjct: 606 WWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFPRT 561

BLAST of Clc09G08840 vs. TAIR 10
Match: AT1G68420.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 4.5e-17
Identity = 37/77 (48.05%), Postives = 59/77 (76.62%), Query Frame = 0

Query: 373 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAGLEDAMNCADDLVKFLSKWVLEHCL 432
           +G+VY+  P FRA+K  +++H AE WMVEVE+AFAG+E+AMNC++ +VK +   +LE C 
Sbjct: 1   MGDVYTFAPTFRAEKSHTSRHLAEFWMVEVELAFAGVEEAMNCSEAVVKDMCTTLLEKCR 60

Query: 433 DDMKFVQKRIDKASIHR 450
           DDM+++ +++D+  I R
Sbjct: 61  DDMEYMVEKVDEFCIDR 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899280.10.0e+0089.46asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida][more]
TYJ96325.10.0e+0086.07asparagine--tRNA ligase [Cucumis melo var. makuwa][more]
XP_008458080.10.0e+0086.07PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo][more]
XP_004149331.17.4e-30985.29asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus][more]
KAA0026087.11.3e-30885.45asparagine--tRNA ligase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9SW951.9e-16149.08Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 ... [more]
Q9SW964.7e-12840.94Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ... [more]
Q9SSK11.8e-11944.49Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ... [more]
Q6MEC94.1e-9237.24Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
P522761.6e-8838.08Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
Match NameE-valueIdentityDescription
A0A5D3BD070.0e+0086.07Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3C6Z50.0e+0086.07Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1[more]
A0A0A0K8G13.6e-30985.29Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1[more]
A0A5A7SMX66.2e-30985.45Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1KTV93.8e-29882.13asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G07420.11.3e-16249.08asparaginyl-tRNA synthetase 2 [more]
AT5G56680.13.3e-12940.94Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.11.3e-12044.49Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.12.3e-8535.95Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G68420.14.5e-1748.05Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 286..323
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 194..644
e-value: 2.6E-80
score: 272.3
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 16..645
NoneNo IPR availablePANTHERPTHR22594:SF36ASPARAGINE--TRNA LIGASE, CYTOPLASMIC 2-RELATEDcoord: 16..645
NoneNo IPR availableCDDcd04318EcAsnRS_like_Ncoord: 124..187
e-value: 2.67797E-16
score: 72.2128
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 202..639
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 374..387
score: 32.47
coord: 358..370
score: 41.78
coord: 600..614
score: 33.94
coord: 560..576
score: 31.82
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 203..264
e-value: 1.6E-7
score: 30.7
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 347..638
e-value: 1.5E-49
score: 168.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc09G08840.2Clc09G08840.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding