Clc09G07970 (gene) Watermelon (cordophanus) v2

BLAST of Clc09G07970 vs. NCBI nr
Match: XP_038897058.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 652.1 bits (1681), Expect = 2.6e-183
Identity = 313/340 (92.06%), Postives = 324/340 (95.29%), Query Frame = 0

Query: 1   MGDA-KFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDA 60
           MGDA KFV+NWEGV+SVPESYVYPP KRPGNVVVP ENAIPVIDLS  DR LL+R ILDA
Sbjct: 1   MGDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDA 60

Query: 61  SKEFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLE 120
           SKEFGFFQVINHGVSK +S ETM+IF EFHAMSGPEKAKECSKDPN SCRVYTSSENY++
Sbjct: 61  SKEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMK 120

Query: 121 EQTHCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG 180
           EQ HCWRDALIFNCHPLEKYV FWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG
Sbjct: 121 EQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG 180

Query: 181 KGYFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIG 240
           KGYF GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDG+WIG
Sbjct: 181 KGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIG 240

Query: 241 VQPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCL 300
           VQPIDNAFVVNIGFVLQ+ITNGKLKAAEHR VTNAKTSRQSLTYLVYPKDEATMEPAKC+
Sbjct: 241 VQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCM 300

Query: 301 INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQS 340
           INEANP RYRSLNFKDFQRNYLPRAVDTKAVM+YIGSNQS
Sbjct: 301 INEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 340

BLAST of Clc09G07970 vs. NCBI nr
Match: XP_004146985.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_016058 [Cucumis sativus])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 309/338 (91.42%), Postives = 319/338 (94.38%), Query Frame = 0

Query: 2   GDAKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDASK 61
           G AKFVSNWE VESVPESYVYPPEKRPGN+VVP   AIPVIDLS  DRTLLVR ILDASK
Sbjct: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63

Query: 62  EFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQ 121
           EFGFFQ+INHGVSK +SEETM+IF EFHAMSGPEKAKECSKDPN SCRVYTSSENY +EQ
Sbjct: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123

Query: 122 THCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 181
            HCWRDALIFNCHPLEKYV FWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGLGKG
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 241
           YF GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLIN 301
           PIDNAFVVNIGFVLQIITNGKL+AAEHR VTN+KTSRQSLTYLVYPKDEA MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303

Query: 302 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQS 340
           EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYI +NQS
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Clc09G07970 vs. NCBI nr
Match: XP_008451261.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 637.5 bits (1643), Expect = 6.6e-179
Identity = 307/338 (90.83%), Postives = 315/338 (93.20%), Query Frame = 0

Query: 2   GDAKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDASK 61
           GDAKFVSNWE  ESVPESYVYPPEKRPGNVVVP   AIPVIDLS  DR LLVR IL ASK
Sbjct: 4   GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63

Query: 62  EFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQ 121
           E GFFQ+INHGVSK +SE TM+IF EFHAMSGPEKAKECSKDPN SCRVYTSSENY +EQ
Sbjct: 64  ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123

Query: 122 THCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 181
            HCWRDALIFNCHPLEKYV FWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 241
           Y  GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLIN 301
           PID AFVVNIGFVLQIITNGKL+AAEHR VTN+KTSRQSLTYLVYPKDEATMEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303

Query: 302 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQS 340
           EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYI +NQS
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Clc09G07970 vs. NCBI nr
Match: KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 616.7 bits (1589), Expect = 1.2e-172
Identity = 290/338 (85.80%), Postives = 312/338 (92.31%), Query Frame = 0

Query: 1   MGDAKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDAS 60
           MG+ KFVSNWEGV+SVP  YVYPPEKRPGNVVVP  NAIPV+DLST DR LLVR ILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEE 120
           +EFGFFQVINHGVS+ + EET +IF +FHAMSG EK KECS+DPN SCRVYTSSENY +E
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGK 180
           QTHCWRDALIFNCHPL+KYV FWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
           GY  GEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 QPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLI 300
            PIDNAFVVNIGFVLQ+I+NGKLKAAEHRVVTNAK SRQSLTYLVYPKDE TMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQ 339
           NEANPPRYR+L FKDFQRNYLPRA DTKAVM+ IGS++
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

BLAST of Clc09G07970 vs. NCBI nr
Match: XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 616.7 bits (1589), Expect = 1.2e-172
Identity = 290/338 (85.80%), Postives = 312/338 (92.31%), Query Frame = 0

Query: 1   MGDAKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDAS 60
           MG+ KFVSNWEGV+SVP  YVYPPEKRPGNVVVP  NAIPV+DLSTHDR LLVR ILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEE 120
           +EFGFFQVINHGVS+ + EET +IF +FHAMSG EK KECS+DPN SCRVYTSSENY +E
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGK 180
           QTHCWRDALIFNCHPL+KYV FWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
           GY  GEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 QPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLI 300
            PIDNAFVVNIGFVLQ+I+NGKLKAAEHRVVTNAK SRQSLTYLVYPKDE TMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQ 339
           NEANPPRYR+L FKDFQRNYLPRA DTKAVM+ IGS++
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

BLAST of Clc09G07970 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.8e-71
Identity = 147/329 (44.68%), Postives = 200/329 (60.79%), Query Frame = 0

Query: 4   AKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDASKEF 63
           A FVSNW   +SV ES++ P +KR     VP  N +P+IDL  H   LLV+ I  A ++F
Sbjct: 2   ATFVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDF 61

Query: 64  GFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAK-----ECSK---DPNGSCRVYTSSE 123
           G FQVINHG  + +  ETM++  EF A+   EK K     E +K         ++Y   E
Sbjct: 62  GLFQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGE 121

Query: 124 NYLEEQTHCWRDALIFNCHPLEK-YVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSE 183
               E+   W+D L   CHPL++  V  WP+ P KYREVV  Y V +RKL + +L+ + E
Sbjct: 122 QLSNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICE 181

Query: 184 GLGLGKGYFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQ--VL 243
           GLGL  GYF  E+S+  ++L N+YPPCP+PS TLG   H D +LIT+L QD+ GLQ  ++
Sbjct: 182 GLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIV 241

Query: 244 KDGQWIGVQPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEAT 303
           KD  WI VQPI  AFVVN+G  L++ITN K + + HRVVT+    R S+  L+ P    T
Sbjct: 242 KDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCT 301

Query: 304 MEPAKCLINEANPPRYRSLNFKDFQRNYL 322
           +EPAK L+N+ NPP Y+  ++ +F   YL
Sbjct: 302 IEPAKELLNQDNPPLYKPYSYSEFADIYL 327

BLAST of Clc09G07970 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.4e-67
Identity = 141/340 (41.47%), Postives = 198/340 (58.24%), Query Frame = 0

Query: 4   AKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDASKEF 63
           AK +S      ++PE+YV P   RP    V      P+IDLS+ DR+ L++ I  A   F
Sbjct: 3   AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62

Query: 64  GFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQTH 123
           GFFQVINHGV+K I +E + +  EF +MS  EK K  S DP  + R+ TS  N  +E+ +
Sbjct: 63  GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122

Query: 124 CWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYF 183
            WRD L  +C+P+ KYV  WP NPP ++E+V  Y   +R++  +I EL+SE LGL K Y 
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182

Query: 184 AGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGV 243
              + E    + VN+YPPCP P LT GL  H DP+ +TIL QD  V GLQ+L DGQW  V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242

Query: 244 QPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCL- 303
            P  +AFV+NIG  LQ ++NG  K+  HR VTN +  R S+   + P D A M PAK L 
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302

Query: 304 --INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSN 338
              ++   P Y+   + ++ + +  R +D +  ++   +N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of Clc09G07970 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.1e-65
Identity = 126/322 (39.13%), Postives = 187/322 (58.07%), Query Frame = 0

Query: 14  ESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDASKEFGFFQVINHGV 73
           +++P  YV P  +RP   +V ++  IPV+DL++ DR  +V  + DA +  GFFQV+NHG+
Sbjct: 18  DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77

Query: 74  SKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQTHCWRDALIFNC 133
              +    M++  EF  +   EKAK  S DP    R+ TS  N  +E  H WRD L  +C
Sbjct: 78  DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHC 137

Query: 134 HPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFAGEMSENPL- 193
           +PL ++V  WP NPP ++E++G YC  +R+L   + E +SE LGL  GY    + E    
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197

Query: 194 LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGVQPIDNAFVVN 253
           + VN+YP CP P LT GL  H DP+ +TIL  D  V GLQVL DG+WI V P   A V+N
Sbjct: 198 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 257

Query: 254 IGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLINEANPPRYRS 313
           IG  LQ ++NGK ++  HR V N+   R S+   + P +   + PAK LI + +P  YR+
Sbjct: 258 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 317

Query: 314 LNFKDFQRNYLPRAVDTKAVMQ 333
             + ++ + +  R +D +  ++
Sbjct: 318 YTYDEYYKKFWSRNLDQEHCLE 338

BLAST of Clc09G07970 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.9e-57
Identity = 126/327 (38.53%), Postives = 184/327 (56.27%), Query Frame = 0

Query: 13  VESVPESYVYPPEKRPGNVVVPTE-NAIPVIDLSTHD-----RTLLVRNILDASKEFGFF 72
           V+ VP +YV P   RP    V T  ++IP+IDL  HD     R  ++     A    GFF
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74

Query: 73  QVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQTHCWR 132
           Q+ NHGV +   ++ M    EF   S  E+ K  S D   + R+ TS  N  +E+   WR
Sbjct: 75  QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWR 134

Query: 133 DALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFAGE 192
           D L  +C+P+E ++  WP  P  +REV   Y  ++R LVL +LE +SE LGL K   +  
Sbjct: 135 DFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNT 194

Query: 193 MSEN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPID 252
           + ++   + +N+YP CP P LT GL  H D +LIT+L QD V+GLQV KDG+WI V P+ 
Sbjct: 195 IGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVP 254

Query: 253 NAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLINEA- 312
           N F+VN+G  +Q+I+N K K+  HR V N+   R S+     P ++A + PA+ LINE  
Sbjct: 255 NTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEE 314

Query: 313 -NPPRYRSLNFKDFQRNYLPRAVDTKA 330
            +P  YR+  + ++   +   A DT++
Sbjct: 315 DSPAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of Clc09G07970 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 223.0 bits (567), Expect = 5.1e-57
Identity = 125/312 (40.06%), Postives = 179/312 (57.37%), Query Frame = 0

Query: 15  SVPESYVYPPEKRPGNVVVPTENAI---PVIDLSTHDRTLLVRNILDASKEFGFFQVINH 74
           S+PES+V+  +K P     P   A+   P+IDLS   R  + R+IL+A KE GFFQV+NH
Sbjct: 13  SLPESFVFASDKVP-----PATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNH 72

Query: 75  GVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQTHCWRDALIF 134
           GVSK +  +   +  +F  +   +KA   S++ +   R++ S   Y       WRD L  
Sbjct: 73  GVSKQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLF-SGATYDTGGEKYWRDCLRL 132

Query: 135 NC-HPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFAGEMS-E 194
            C  P++  +  WP  P   R+V+  +    R +  E+L L+ EG+G+   YF G++S  
Sbjct: 133 ACPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGG 192

Query: 195 NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNAFV 254
           N +L +NHYP CPNP   LG   HCD +LIT+L    VNGL+V   G WI V P  NAFV
Sbjct: 193 NVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFV 252

Query: 255 VNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLINEANPPRY 314
           VN G  L+++TNG LK+ EHR +TN+  +R S+   + P  E  + PAK  +++ NPP Y
Sbjct: 253 VNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCY 312

Query: 315 RSLNFKDFQRNY 321
           R+  F+DF R Y
Sbjct: 313 RTTMFRDFMRIY 317

BLAST of Clc09G07970 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.2e-182
Identity = 309/338 (91.42%), Postives = 319/338 (94.38%), Query Frame = 0

Query: 2   GDAKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDASK 61
           G AKFVSNWE VESVPESYVYPPEKRPGN+VVP   AIPVIDLS  DRTLLVR ILDASK
Sbjct: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63

Query: 62  EFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQ 121
           EFGFFQ+INHGVSK +SEETM+IF EFHAMSGPEKAKECSKDPN SCRVYTSSENY +EQ
Sbjct: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123

Query: 122 THCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 181
            HCWRDALIFNCHPLEKYV FWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGLGKG
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 241
           YF GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLIN 301
           PIDNAFVVNIGFVLQIITNGKL+AAEHR VTN+KTSRQSLTYLVYPKDEA MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303

Query: 302 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQS 340
           EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYI +NQS
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Clc09G07970 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.2e-179
Identity = 307/338 (90.83%), Postives = 315/338 (93.20%), Query Frame = 0

Query: 2   GDAKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDASK 61
           GDAKFVSNWE  ESVPESYVYPPEKRPGNVVVP   AIPVIDLS  DR LLVR IL ASK
Sbjct: 4   GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63

Query: 62  EFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQ 121
           E GFFQ+INHGVSK +SE TM+IF EFHAMSGPEKAKECSKDPN SCRVYTSSENY +EQ
Sbjct: 64  ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123

Query: 122 THCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 181
            HCWRDALIFNCHPLEKYV FWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 241
           Y  GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLIN 301
           PID AFVVNIGFVLQIITNGKL+AAEHR VTN+KTSRQSLTYLVYPKDEATMEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303

Query: 302 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQS 340
           EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYI +NQS
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Clc09G07970 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.8e-173
Identity = 290/338 (85.80%), Postives = 312/338 (92.31%), Query Frame = 0

Query: 1   MGDAKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDAS 60
           MG+ KFVSNWEGV+SVP  YVYPPEKRPGNVVVP  NAIPV+DLSTHDR LLVR ILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEE 120
           +EFGFFQVINHGVS+ + EET +IF +FHAMSG EK KECS+DPN SCRVYTSSENY +E
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGK 180
           QTHCWRDALIFNCHPL+KYV FWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
           GY  GEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 QPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLI 300
            PIDNAFVVNIGFVLQ+I+NGKLKAAEHRVVTNAK SRQSLTYLVYPKDE TMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQ 339
           NEANPPRYR+L FKDFQRNYLPRA DTKAVM+ IGS++
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

BLAST of Clc09G07970 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.6e-173
Identity = 292/339 (86.14%), Postives = 311/339 (91.74%), Query Frame = 0

Query: 1   MGDAKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDAS 60
           MG+ KFVSNWEGV+SVP  Y+YPPEKRPGNVVVP  NAIPV+DLST DR LLVR ILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEE 120
           +EFGFFQVINHGVS+ + EET +IF +FHAMSG EKAKECS+DP  SCRVYTSSENY +E
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGK 180
           QTHCWRDALIFNCHPLEKYV FWPQNPPKYREVVGAYCVAMR LVLEILEL+SEGLGLG+
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGV 240
           GY A EMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 QPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLI 300
            PIDNAFVVNIGFVLQ+I+NGKLKAAEHR VTNAK SRQSLTYLVYPKDE TMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQS 340
           NEANPPRYR+L FKDFQRNYLPRA DTKAVM+ IGSN S
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339

BLAST of Clc09G07970 vs. ExPASy TrEMBL
Match: A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.6e-167
Identity = 279/340 (82.06%), Postives = 304/340 (89.41%), Query Frame = 0

Query: 1   MGD-AKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDA 60
           MGD   FVSNW GVESVPESYVYPPEKRPGNV VP ENAIPV+DL+ HDR  LV  ILDA
Sbjct: 1   MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60

Query: 61  SKEFGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLE 120
           S+EFGFFQVINHGVSK + EETM++F EFHAMSGPEK +ECSKDPN SCRVYTSSEN+++
Sbjct: 61  SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120

Query: 121 EQTHCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG 180
           E+THCWRDALIFNCHPL+KYV FWPQNP  YREVVGAYC+ MRKLV+EILEL+SEGLGLG
Sbjct: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180

Query: 181 KGYFAGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIG 240
           KGYF  EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ +NGLQVLKDGQWIG
Sbjct: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240

Query: 241 VQPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCL 300
           V P+DNAFVVNIGFVLQ+++NGKLKA EHR V NAK SRQS+TYLVYPKD+ATMEPAKC+
Sbjct: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300

Query: 301 INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSNQS 340
           INEANP  Y SL FKDFQRNYLP A DTKAV+QYI S+ S
Sbjct: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340

BLAST of Clc09G07970 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.7e-68
Identity = 141/340 (41.47%), Postives = 198/340 (58.24%), Query Frame = 0

Query: 4   AKFVSNWEGVESVPESYVYPPEKRPGNVVVPTENAIPVIDLSTHDRTLLVRNILDASKEF 63
           AK +S      ++PE+YV P   RP    V      P+IDLS+ DR+ L++ I  A   F
Sbjct: 3   AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62

Query: 64  GFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQTH 123
           GFFQVINHGV+K I +E + +  EF +MS  EK K  S DP  + R+ TS  N  +E+ +
Sbjct: 63  GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122

Query: 124 CWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYF 183
            WRD L  +C+P+ KYV  WP NPP ++E+V  Y   +R++  +I EL+SE LGL K Y 
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182

Query: 184 AGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGV 243
              + E    + VN+YPPCP P LT GL  H DP+ +TIL QD  V GLQ+L DGQW  V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242

Query: 244 QPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCL- 303
            P  +AFV+NIG  LQ ++NG  K+  HR VTN +  R S+   + P D A M PAK L 
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302

Query: 304 --INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIGSN 338
              ++   P Y+   + ++ + +  R +D +  ++   +N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of Clc09G07970 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.8e-58
Identity = 126/327 (38.53%), Postives = 184/327 (56.27%), Query Frame = 0

Query: 13  VESVPESYVYPPEKRPGNVVVPTE-NAIPVIDLSTHD-----RTLLVRNILDASKEFGFF 72
           V+ VP +YV P   RP    V T  ++IP+IDL  HD     R  ++     A    GFF
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74

Query: 73  QVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQTHCWR 132
           Q+ NHGV +   ++ M    EF   S  E+ K  S D   + R+ TS  N  +E+   WR
Sbjct: 75  QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWR 134

Query: 133 DALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFAGE 192
           D L  +C+P+E ++  WP  P  +REV   Y  ++R LVL +LE +SE LGL K   +  
Sbjct: 135 DFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNT 194

Query: 193 MSEN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPID 252
           + ++   + +N+YP CP P LT GL  H D +LIT+L QD V+GLQV KDG+WI V P+ 
Sbjct: 195 IGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVP 254

Query: 253 NAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLINEA- 312
           N F+VN+G  +Q+I+N K K+  HR V N+   R S+     P ++A + PA+ LINE  
Sbjct: 255 NTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEE 314

Query: 313 -NPPRYRSLNFKDFQRNYLPRAVDTKA 330
            +P  YR+  + ++   +   A DT++
Sbjct: 315 DSPAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of Clc09G07970 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 219.2 bits (557), Expect = 5.2e-57
Identity = 120/332 (36.14%), Postives = 187/332 (56.33%), Query Frame = 0

Query: 7   VSNWEGVESVPESYVYPPEKRPG-NVVVPTENAIPVIDLST---HDRTLLVRNILDASKE 66
           VS++     +P +YV P   RP  + V  + ++IP+IDL      +R ++V+ +  A   
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 67  FGFFQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQT 126
           +GFFQ+ NHGV      +   +  EF      E+ K  S DP  + R+ TS  N   ++ 
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130

Query: 127 HCWRDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGY 186
             WRD L  +C P+E +++ WP +P  +REV   Y  ++R LVL +LE +SE LGL   +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 187 FAGEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGV 246
            +  + ++   +  N+YPPCP P LT GL  H DP++IT+L QD V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 247 QPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLI 306
            PI N F+VNIG  +Q+I+N K K+  HR V N +  R S+    +P  +A + PA  L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

Query: 307 NEANP-PRYRSLNFKDFQRNYLPRAVDTKAVM 332
           NE +    YR+  F ++   +  R++ T + +
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLATASCL 341

BLAST of Clc09G07970 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.7e-53
Identity = 119/318 (37.42%), Postives = 173/318 (54.40%), Query Frame = 0

Query: 12  GVESVPESYVYPPEKRP--GNVVVPTENAIPVIDLS-THD---RTLLVRNILDASKEFGF 71
           GV  VP+ YV PP +RP  G+ +  +E  +PVIDLS  H    R+L +  I  A KEFGF
Sbjct: 23  GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82

Query: 72  FQVINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQTHCW 131
           FQVINHG+   +  + +    +F  +   EK    S + +   R Y +S N+  ++ H W
Sbjct: 83  FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142

Query: 132 RDALIFNCHPLEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFAG 191
           RD +    HPL K++  WP NPP Y++ VG Y  A   L  +++E +SE LGL K Y   
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202

Query: 192 EMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLK-DGQWIGVQPI 251
           E+ E + ++ VN YP CP P + LG+  H D S +TIL Q   GLQ++  +  W+ V  I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262

Query: 252 DNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKDEATMEPAKCLINEA 311
           + A +V +G  +++++NG  K+  HRV  N +  R S   L        + PA  L+N  
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPN 322

Query: 312 NPPRYRSLNFKDFQRNYL 322
           N P Y   +F DF  NY+
Sbjct: 323 NAPAYGEFSFNDF-LNYI 338

BLAST of Clc09G07970 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.2e-50
Identity = 122/327 (37.31%), Postives = 170/327 (51.99%), Query Frame = 0

Query: 18  ESYVYPPEKRPGNVVVPT-----ENAIPVIDLST-----HDRTLLVRNILDASKEFGFFQ 77
           E+++  PE RP   +  +      + IP IDLS+     HD+T + + I +A K +GFFQ
Sbjct: 6   EAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQ 65

Query: 78  VINHGVSKMISEETMKIFNEFHAMSGPEKAKECSKDPNGSCRVYTSSENYLEEQTHCWR- 137
           VINHG+   +     K   EF  ++  EK K    + N     Y   E+   +    W+ 
Sbjct: 66  VINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNP--MGYHDEEH--TKNVRDWKE 125

Query: 138 -------DALIFNCHP------LEKYVQFWPQNPPKYREVVGAYCVAMRKLVLEILELMS 197
                  D+ I    P      L K    WPQNP  +REV   Y   + KL   +LEL+S
Sbjct: 126 IFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVS 185

Query: 198 EGLGLGKGYFAGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQV 257
             LGL      G  +E    L  NHYPPCPNP L LG+ +H D   +T+L QD V GLQV
Sbjct: 186 ISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQV 245

Query: 258 LK--DGQWIGVQPIDNAFVVNIGFVLQIITNGKLKAAEHRVVTNAKTSRQSLTYLVYPKD 317
            +  DGQWI V+PI +A ++N+G  +Q+ TN +  +AEHRVV N    R S+ +  +P  
Sbjct: 246 SRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSH 305