Clc09G03800 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc09G03800
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	RING/U-box superfamily protein
Location	ClcChr09: 2928528 .. 2928827 (-)
RNA-Seq Expression	Clc09G03800
Synteny	Clc09G03800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTACCAAAGGAAATAACGGAAAGGTGGAACAAAGCGCTTTATTTTGAAGCAGTGGAAGTGTTTTGTCCATTCAAGGATTGCTCCGAGGGAATGATCCTTGAGAAGGAAGCAACGGGCGGAGGAGGCAAATTACGGGATTGTGAATGTCCGTTTTGCCATAGGCTGTTCTGCGCCACGTGCAAAGTCCCATGGCACGAGGGGATCACGTGCGACGAATACCAGACGCTTTATCAAGCAGATCAGAGGGGTAGAGACGACATTTTGATGAAAATGTTGGCCTCCAAAATGAAAAGATGA

mRNA sequence

ATGCTACCAAAGGAAATAACGGAAAGGTGGAACAAAGCGCTTTATTTTGAAGCAGTGGAAGTGTTTTGTCCATTCAAGGATTGCTCCGAGGGAATGATCCTTGAGAAGGAAGCAACGGGCGGAGGAGGCAAATTACGGGATTGTGAATGTCCGTTTTGCCATAGGCTGTTCTGCGCCACGTGCAAAGTCCCATGGCACGAGGGGATCACGTGCGACGAATACCAGACGCTTTATCAAGCAGATCAGAGGGGTAGAGACGACATTTTGATGAAAATGTTGGCCTCCAAAATGAAAAGATGA

Coding sequence (CDS)

ATGCTACCAAAGGAAATAACGGAAAGGTGGAACAAAGCGCTTTATTTTGAAGCAGTGGAAGTGTTTTGTCCATTCAAGGATTGCTCCGAGGGAATGATCCTTGAGAAGGAAGCAACGGGCGGAGGAGGCAAATTACGGGATTGTGAATGTCCGTTTTGCCATAGGCTGTTCTGCGCCACGTGCAAAGTCCCATGGCACGAGGGGATCACGTGCGACGAATACCAGACGCTTTATCAAGCAGATCAGAGGGGTAGAGACGACATTTTGATGAAAATGTTGGCCTCCAAAATGAAAAGATGA

Protein sequence

MLPKEITERWNKALYFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRLFCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMKR

Homology

[Close]

BLAST of Clc09G03800 vs. NCBI nr
Match: TYK10490.1 (putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa])

HSP 1 Score: 139.8 bits (351), Expect = 1.3e-29
Identity = 63/102 (61.76%), Postives = 79/102 (77.45%), Query Frame = 0


Query: 1   MLPKEITERWNKAL----YFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           MLP E  E+WNKAL    Y E  +V CPFKDCS GMI+E++A     K+RDCECP CHRL
Sbjct: 66  MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDA-----KIRDCECPLCHRL 125

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
           FC TC+VPWHEG+TC++Y+ LY+ ++ GRD+ILMK LAS+MK
Sbjct: 126 FCGTCRVPWHEGVTCEDYKRLYKYEE-GRDEILMKKLASQMK 161

[Close]

BLAST of Clc09G03800 vs. NCBI nr
Match: KAA0058684.1 (putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa])

HSP 1 Score: 139.0 bits (349), Expect = 2.1e-29
Identity = 63/102 (61.76%), Postives = 78/102 (76.47%), Query Frame = 0


Query: 1   MLPKEITERWNKAL----YFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           MLP E  E+WNKAL    Y E  +V CPFKDCS GMI+E++A     K+RDCECP CHRL
Sbjct: 66  MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDA-----KIRDCECPLCHRL 125

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
           FC TC+VPWHEG+TC++Y+ LY+ +  GRD+ILMK LAS+MK
Sbjct: 126 FCGTCRVPWHEGVTCEDYKRLYKYED-GRDEILMKKLASQMK 161

[Close]

BLAST of Clc09G03800 vs. NCBI nr
Match: KAE8646419.1 (hypothetical protein Csa_016113 [Cucumis sativus])

HSP 1 Score: 136.0 bits (341), Expect = 1.8e-28
Identity = 62/102 (60.78%), Postives = 78/102 (76.47%), Query Frame = 0


Query: 1   MLPKEITERWNKA----LYFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           MLPKEI E+WNKA    LY    +V CPFKDCS G+ LE+     G  +RDCECP CHRL
Sbjct: 50  MLPKEIGEKWNKALCEVLYSTEEKVVCPFKDCSVGITLER-----GASIRDCECPLCHRL 109

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
           FCATC+VPWH+G+TC+EY+ LY+ ++ GR++ILMK LAS+MK
Sbjct: 110 FCATCEVPWHDGVTCEEYKRLYK-NEEGRNEILMKKLASQMK 145

[Close]

BLAST of Clc09G03800 vs. NCBI nr
Match: XP_008461214.1 (PREDICTED: probable E3 ubiquitin-protein ligase RNF217 [Cucumis melo])

HSP 1 Score: 134.8 bits (338), Expect = 4.0e-28
Identity = 61/102 (59.80%), Postives = 78/102 (76.47%), Query Frame = 0


Query: 1  MLPKEITERWNKAL----YFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
          MLP E  E+WNKAL    Y E  +V CPFKDCS GMI+E++A     K+RDCECP CHRL
Sbjct: 1  MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDA-----KIRDCECPLCHRL 60

Query: 61 FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
          FC TC+VPWHEG+TC++Y+ LY+ ++  RD+ILM+ LAS+MK
Sbjct: 61 FCGTCRVPWHEGVTCEDYKRLYKYEEI-RDEILMEKLASQMK 96

[Close]

BLAST of Clc09G03800 vs. NCBI nr
Match: KEH43916.1 (C6HC-type zinc finger RING/U-box protein [Medicago truncatula] >RHN82080.1 putative IBR domain, E3 ubiquitin ligase RBR family [Medicago truncatula])

HSP 1 Score: 117.1 bits (292), Expect = 8.7e-23
Identity = 53/102 (51.96%), Postives = 73/102 (71.57%), Query Frame = 0


Query: 1   MLPKEITERWN----KALYFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           +LPKE+ ++WN    +AL+    + +CPFKDCS  ++ E E   G   +R+ ECPFCHRL
Sbjct: 3   LLPKELFDKWNDALCEALFVTVPKFYCPFKDCSAMLLDENE---GVEDIRESECPFCHRL 62

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
           FCA C VPWH GI C+EYQTL  AD+RGR+D+L++ LA++ K
Sbjct: 63  FCARCHVPWHPGIDCEEYQTL-NADERGREDLLVRELANEKK 100

[Close]

BLAST of Clc09G03800 vs. ExPASy Swiss-Prot
Match: Q8BKD6 (E3 ubiquitin-protein ligase RNF144B OS=Mus musculus OX=10090 GN=Rnf144b PE=2 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 2.2e-05
Identity = 20/68 (29.41%), Postives = 35/68 (51.47%), Query Frame = 0


Query: 9   RWNKALYFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRLFCATCKVPWHEG 68
           ++ + ++ + +  +CP  DC     +   + G  G+    ECP CH  FC+ CK  WHE 
Sbjct: 107 KFEREVHMDPLRTWCPVADCQTVCHI---SAGDPGQPVLVECPSCHLKFCSCCKDAWHEE 166

Query: 69  ITCDEYQT 77
            +C + Q+
Sbjct: 167 SSCRDSQS 171

[Close]

BLAST of Clc09G03800 vs. ExPASy TrEMBL
Match: A0A5D3CGK1 (Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001400 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 6.1e-30
Identity = 63/102 (61.76%), Postives = 79/102 (77.45%), Query Frame = 0


Query: 1   MLPKEITERWNKAL----YFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           MLP E  E+WNKAL    Y E  +V CPFKDCS GMI+E++A     K+RDCECP CHRL
Sbjct: 66  MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDA-----KIRDCECPLCHRL 125

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
           FC TC+VPWHEG+TC++Y+ LY+ ++ GRD+ILMK LAS+MK
Sbjct: 126 FCGTCRVPWHEGVTCEDYKRLYKYEE-GRDEILMKKLASQMK 161

[Close]

BLAST of Clc09G03800 vs. ExPASy TrEMBL
Match: A0A5A7UU31 (Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001540 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-29
Identity = 63/102 (61.76%), Postives = 78/102 (76.47%), Query Frame = 0


Query: 1   MLPKEITERWNKAL----YFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           MLP E  E+WNKAL    Y E  +V CPFKDCS GMI+E++A     K+RDCECP CHRL
Sbjct: 66  MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDA-----KIRDCECPLCHRL 125

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
           FC TC+VPWHEG+TC++Y+ LY+ +  GRD+ILMK LAS+MK
Sbjct: 126 FCGTCRVPWHEGVTCEDYKRLYKYED-GRDEILMKKLASQMK 161

[Close]

BLAST of Clc09G03800 vs. ExPASy TrEMBL
Match: A0A0A0K9X9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430760 PE=3 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 8.8e-29
Identity = 62/102 (60.78%), Postives = 78/102 (76.47%), Query Frame = 0


Query: 1   MLPKEITERWNKA----LYFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           MLPKEI E+WNKA    LY    +V CPFKDCS G+ LE+     G  +RDCECP CHRL
Sbjct: 66  MLPKEIGEKWNKALCEVLYSTEEKVVCPFKDCSVGITLER-----GASIRDCECPLCHRL 125

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
           FCATC+VPWH+G+TC+EY+ LY+ ++ GR++ILMK LAS+MK
Sbjct: 126 FCATCEVPWHDGVTCEEYKRLYK-NEEGRNEILMKKLASQMK 161

[Close]

BLAST of Clc09G03800 vs. ExPASy TrEMBL
Match: A0A1S3CDQ5 (probable E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo OX=3656 GN=LOC103499864 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.0e-28
Identity = 61/102 (59.80%), Postives = 78/102 (76.47%), Query Frame = 0


Query: 1  MLPKEITERWNKAL----YFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
          MLP E  E+WNKAL    Y E  +V CPFKDCS GMI+E++A     K+RDCECP CHRL
Sbjct: 1  MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDA-----KIRDCECPLCHRL 60

Query: 61 FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
          FC TC+VPWHEG+TC++Y+ LY+ ++  RD+ILM+ LAS+MK
Sbjct: 61 FCGTCRVPWHEGVTCEDYKRLYKYEEI-RDEILMEKLASQMK 96

[Close]

BLAST of Clc09G03800 vs. ExPASy TrEMBL
Match: A0A072VQJ6 (C6HC-type zinc finger RING/U-box protein OS=Medicago truncatula OX=3880 GN=MTR_1g105450 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.2e-23
Identity = 53/102 (51.96%), Postives = 73/102 (71.57%), Query Frame = 0


Query: 1   MLPKEITERWN----KALYFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           +LPKE+ ++WN    +AL+    + +CPFKDCS  ++ E E   G   +R+ ECPFCHRL
Sbjct: 3   LLPKELFDKWNDALCEALFVTVPKFYCPFKDCSAMLLDENE---GVEDIRESECPFCHRL 62

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
           FCA C VPWH GI C+EYQTL  AD+RGR+D+L++ LA++ K
Sbjct: 63  FCARCHVPWHPGIDCEEYQTL-NADERGREDLLVRELANEKK 100

[Close]

BLAST of Clc09G03800 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 2.5e-20
Identity = 43/102 (42.16%), Postives = 63/102 (61.76%), Query Frame = 0


Query: 1   MLPKEITERWNKAL----YFEAVEVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           +LPKE+ +RW  AL       + + +CP+KDCS  + LE+       K++D ECP CHR+
Sbjct: 176 ILPKEVFDRWGDALCEAVVMRSKKFYCPYKDCSALVFLEESEV----KMKDSECPHCHRM 235

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
            C  C   WH  +TC+E+Q L  A++RGRDDIL+  +A + K
Sbjct: 236 VCVECGTQWHPEMTCEEFQKL-AANERGRDDILLATMAKQKK 272

[Close]

BLAST of Clc09G03800 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 83.6 bits (205), Expect = 1.0e-16
Identity = 39/106 (36.79%), Postives = 58/106 (54.72%), Query Frame = 0


Query: 1   MLPKEITERWNKALYFEAV----EVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           ++PK++ +RW K L    +    + +CPFKDCS  M+  +    G   +   EC  CHRL
Sbjct: 157 LIPKDVFDRWEKILCESLISSWDKFYCPFKDCSAMMVNNE---NGDANVTQTECRSCHRL 216

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDD---ILMKMLASKMKR 100
           FC  CKV WH GI CDE+Q      ++  D+   +L++M  +K  R
Sbjct: 217 FCVQCKVTWHAGIGCDEFQRFGNTKKKSSDEDDALLIQMAKNKQWR 259

[Close]

BLAST of Clc09G03800 vs. TAIR 10
Match: AT3G45470.1 (IBR domain containing protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.4e-10
Identity = 31/102 (30.39%), Postives = 51/102 (50.00%), Query Frame = 0


Query: 1   MLPKEITERWNKALYFEAV----EVFCPFKDCSEGMILEKEATGGGGKLRDCECPFCHRL 60
           +L  ++ E W   +  E++     ++CP+ +CS   ++ K       K  D  C  C  L
Sbjct: 66  VLKPKVIELWKHMMKEESIPLAKRIYCPYINCS--TLMSKTEISRSNKSNDRACIKCSGL 125

Query: 61  FCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLASKMK 99
            C  CKVPWH  ++C EY+ L+       DD+ +K+LA+  K
Sbjct: 126 VCIDCKVPWHSDLSCAEYKKLH--PDPVLDDLTLKLLANDQK 163

[Close]

BLAST of Clc09G03800 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 5.3e-10
Identity = 30/100 (30.00%), Postives = 57/100 (57.00%), Query Frame = 0


Query: 1   MLPKEITERWNKA----LYFEAVEVFCPFKDCSEGMILEK-EATGGGGKLRDCECPFCHR 60
           +L  ++ E W +     L  +A +++CP++ CS  M++ K E +    +     C  C  
Sbjct: 241 VLTPKLIEMWKRKMEEDLIPDAEKIYCPYRSCS--MLMSKTELSREAEQSNVRACIKCSE 300

Query: 61  LFCATCKVPWHEGITCDEYQTLYQADQRGRDDILMKMLAS 96
           LFC  CKVPWH  ++C +Y+ ++   +R  +D+++K+LA+
Sbjct: 301 LFCIDCKVPWHSDLSCADYKRIH--SERLVNDMMLKVLAN 336

[Close]

BLAST of Clc09G03800 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 2.0e-09
Identity = 28/86 (32.56%), Postives = 43/86 (50.00%), Query Frame = 0


Query: 20  EVFCPFKDCSEGMILEKEATGGGGKLRD-----------CECPFCHRLFCATCKVPWHEG 79
           +++CP+ +CS   +L+ +     G+               ECP C R  C  C VPWH  
Sbjct: 288 KIYCPYPNCS--FLLDPQECLSSGRASSSSSTQSENSCCVECPVCERFVCVDCGVPWHAS 347

Query: 80  ITCDEYQTLYQADQRGRDDILMKMLA 95
           ++C+E+Q L   D+R  DDI +  LA
Sbjct: 348 MSCEEFQIL-PVDERYPDDITLHRLA 370

The following BLAST results are available for this feature:

BLAST of Clc09G03800 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK10490.1	1.3e-29	61.76	putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa]	[more]
KAA0058684.1	2.1e-29	61.76	putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa]	[more]
KAE8646419.1	1.8e-28	60.78	hypothetical protein Csa_016113 [Cucumis sativus]	[more]
XP_008461214.1	4.0e-28	59.80	PREDICTED: probable E3 ubiquitin-protein ligase RNF217 [Cucumis melo]	[more]
KEH43916.1	8.7e-23	51.96	C6HC-type zinc finger RING/U-box protein [Medicago truncatula] >RHN82080.1 putat...	[more]

BLAST of Clc09G03800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8BKD6	2.2e-05	29.41	E3 ubiquitin-protein ligase RNF144B OS=Mus musculus OX=10090 GN=Rnf144b PE=2 SV=...	[more]

BLAST of Clc09G03800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CGK1	6.1e-30	61.76	Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A5A7UU31	1.0e-29	61.76	Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0K9X9	8.8e-29	60.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430760 PE=3 SV=1	[more]
A0A1S3CDQ5	2.0e-28	59.80	probable E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo OX=3656 GN=LOC103499...	[more]
A0A072VQJ6	4.2e-23	51.96	C6HC-type zinc finger RING/U-box protein OS=Medicago truncatula OX=3880 GN=MTR_1...	[more]

BLAST of Clc09G03800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53690.1	2.5e-20	42.16	RING/U-box superfamily protein	[more]
AT3G14250.1	1.0e-16	36.79	RING/U-box superfamily protein	[more]
AT3G45470.1	1.4e-10	30.39	IBR domain containing protein	[more]
AT5G37560.1	5.3e-10	30.00	RING/U-box superfamily protein	[more]
AT4G19670.1	2.0e-09	32.56	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 8..71 e-value: 1.3E-9 score: 47.9
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 21..71 e-value: 6.7E-10 score: 39.0
None	No IPR available	GENE3D	2.20.25.20		coord: 21..76 e-value: 8.7E-6 score: 27.8
None	No IPR available	PANTHER	PTHR11685:SF336	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 2..98
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 10..76
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 2..98
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 1..99 score: 12.591592

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc09G03800.1	Clc09G03800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0043167	ion binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity