Clc08G17050 (gene) Watermelon (cordophanus) v2

BLAST of Clc08G17050 vs. NCBI nr
Match: XP_038885776.1 (subtilisin-like protease SBT3 [Benincasa hispida])

HSP 1 Score: 1282.3 bits (3317), Expect = 0.0e+00
Identity = 656/746 (87.94%), Postives = 693/746 (92.90%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHT 137
           ETD YIVHMNSAAMPKPFASRHSWYSATISSVLH        SSSSSSSS S  SKL H+
Sbjct: 23  ETDNYIVHMNSAAMPKPFASRHSWYSATISSVLH--------SSSSSSSSASSSSKLTHS 82

Query: 138 YNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISNC 197
           YNHAI+GFSASL+PSELEALKNSPGYVSSVLDSSVRVDTTHSS+FLGLSSNFGLLPISN 
Sbjct: 83  YNHAIDGFSASLSPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFLGLSSNFGLLPISNY 142

Query: 198 GSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKG 257
           GSDVIIGFVDTGIWPE ESFNDDGMSEIPS WKGECE GTHF+ASLCNKKLIGARFFNKG
Sbjct: 143 GSDVIIGFVDTGIWPEGESFNDDGMSEIPSRWKGECERGTHFNASLCNKKLIGARFFNKG 202

Query: 258 LSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYK 317
           L AKFPN+TISMNSTRDTIGHGTHT+T AAGSYV+EASFFGYG+GTARGVAPRAR+AIYK
Sbjct: 203 LLAKFPNITISMNSTRDTIGHGTHTATIAAGSYVKEASFFGYGRGTARGVAPRARIAIYK 262

Query: 318 AIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATS 377
           AIWEEGN+VSDVIAAIDQAISDGVDVISLSLGLDGVPLY+DPVAIATFAAVERGIFVAT+
Sbjct: 263 AIWEEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYQDPVAIATFAAVERGIFVATT 322

Query: 378 AGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLP 437
            GNNGP+LGTVHNGAPWVLNVAA TMDRDFGGTITLSNGV+I GSSLFPLNSTMDVSPLP
Sbjct: 323 TGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVTILGSSLFPLNSTMDVSPLP 382

Query: 438 IVFMGGCQNLKKLKSIGYKIVVCEDKDG-HSLSSQVDNVQSSRVALGIFISNISDWDNLI 497
           IVFMGGCQNLKKLK IGYKI VC+DKD  +SLSSQVDNVQS+ VALG+FISNISDWDNLI
Sbjct: 383 IVFMGGCQNLKKLKRIGYKIAVCDDKDEYYSLSSQVDNVQSANVALGVFISNISDWDNLI 442

Query: 498 KTPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPF 557
           +T FPSIFLSP++GNIIK YIKKSSDPKAEVNF+KTILG KPAPSVARYSSRGPSMSCPF
Sbjct: 443 QTSFPSIFLSPYNGNIIKDYIKKSSDPKAEVNFHKTILGTKPAPSVARYSSRGPSMSCPF 502

Query: 558 VLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHR 617
           VLKPDIMAPGDAILASWPQNVAAM VYS PIYTKFN+MSGTSMSCPHAA VAALLK VH 
Sbjct: 503 VLKPDIMAPGDAILASWPQNVAAMYVYSDPIYTKFNIMSGTSMSCPHAAGVAALLKVVHH 562

Query: 618 RWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMG 677
            WSPA IRSAMMTTADVVDN QTSIKDIGNKN+FA+PLAMGSG +NPNKAIDP L+YDMG
Sbjct: 563 GWSPAAIRSAMMTTADVVDNNQTSIKDIGNKNRFASPLAMGSGHINPNKAIDPGLVYDMG 622

Query: 678 IQDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGE 737
           IQDY+NLLCALNYTK+QIQTIT+  SN CENPS+DLNYPSFIIIVN+S+SKT K EILGE
Sbjct: 623 IQDYVNLLCALNYTKSQIQTITRLDSNDCENPSMDLNYPSFIIIVNTSNSKTSKGEILGE 682

Query: 738 FKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETD 797
           FKRTLTKIGEKRATYEAKLRGMKG KV V PKKLEFKRKNQKLSFKLKIAG+ RVK E D
Sbjct: 683 FKRTLTKIGEKRATYEAKLRGMKGFKVGVNPKKLEFKRKNQKLSFKLKIAGTARVKREND 742

Query: 798 VVYGYLSWAEVGGGHIVQSPIVVASL 823
           +V+GYLSW EVGGGHIVQSPIVVA L
Sbjct: 743 MVFGYLSWVEVGGGHIVQSPIVVAGL 760

BLAST of Clc08G17050 vs. NCBI nr
Match: XP_008444560.1 (PREDICTED: subtilisin-like protease SBT1.9 [Cucumis melo] >KAA0060951.1 subtilisin-like protease SBT1.9 [Cucumis melo var. makuwa])

HSP 1 Score: 1258.8 bits (3256), Expect = 0.0e+00
Identity = 638/745 (85.64%), Postives = 691/745 (92.75%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHT 137
           ET  YIVHMNSAAMPKPFASRHSWYSATISSVLH+S   ++SSSSSSSSS SFPSKLIHT
Sbjct: 23  ETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSS---SSSSSSSSSSSSSFPSKLIHT 82

Query: 138 YNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISNC 197
           YNHAI+GF ASLTPS+LEALKNSPGY+SSVLDSSV VDTTHSS+FLGLSSN GLLPIS  
Sbjct: 83  YNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPISKY 142

Query: 198 GSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKG 257
           GSDVIIGFVDTGIWP+SESFNDDGMSEIPS WKGECE+ THF+ S CN KLIGARFFNKG
Sbjct: 143 GSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGARFFNKG 202

Query: 258 LSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYK 317
           L A+FPN TISMNSTRDTIGHGTHTST AAGSYV+EASFFGYG+GTARGVAPRARVAIYK
Sbjct: 203 LIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGTARGVAPRARVAIYK 262

Query: 318 AIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATS 377
           AIW+EGNSVSDV+AAIDQAISDGVDVISLS+G+DG+PLY DPV+IATFAAVERGIFVATS
Sbjct: 263 AIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIATFAAVERGIFVATS 322

Query: 378 AGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLP 437
           AGNNGP+LGTVHNGAPWVLNVAA TMDRDFGGTITLSNGVS+ GSSLFPLN+TM +SPLP
Sbjct: 323 AGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTMGLSPLP 382

Query: 438 IVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLIK 497
           IVFMGGCQNLKKLK IGYKIVVCED DG+SL+SQVDNVQ+++V LGIFISNI DWDNLI+
Sbjct: 383 IVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTLGIFISNIFDWDNLIQ 442

Query: 498 TPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPFV 557
           TPFPSIF++P+HGNIIK YI KSSDPKAEVNF+KTILG KPAP+VARYSSRGPS SCPFV
Sbjct: 443 TPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTVARYSSRGPSQSCPFV 502

Query: 558 LKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHRR 617
           LKPDIMAPGDAILASWPQN+ AM+V S+PIYTKFNV+SGTSMSCPHAA VAALLKG H +
Sbjct: 503 LKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALLKGAHPQ 562

Query: 618 WSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMGI 677
           WSPA IRSAMMTTADVVDNTQTSIKDIGN NKFATPLAMGSG VNPNKA+DPDLIYD+GI
Sbjct: 563 WSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDLIYDVGI 622

Query: 678 QDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGEF 737
           QDY+N+LCALNYT+NQI+ IT+S SN CENPSLDLNYPSFIII NSSDSKT KR+ILGEF
Sbjct: 623 QDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIANSSDSKTGKRKILGEF 682

Query: 738 KRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETDV 797
           KRTLTKIGE RATYEAKLRGMKG KVRVKP+ LEFKRKNQKLSF+LKIAGS     E++V
Sbjct: 683 KRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAGSAE---ESNV 742

Query: 798 VYGYLSWAEVGGGHIVQSPIVVASL 823
           V+GYLSWAEVGG HIVQSPIVVA +
Sbjct: 743 VFGYLSWAEVGGRHIVQSPIVVAGM 761

BLAST of Clc08G17050 vs. NCBI nr
Match: XP_004143027.3 (subtilisin-like protease SBT3 [Cucumis sativus] >KGN62450.1 hypothetical protein Csa_018640 [Cucumis sativus])

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 620/745 (83.22%), Postives = 673/745 (90.34%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHT 137
           ET  YIVHMNSAAMPKPFASRHSWYSATISS+LH          SSSSSS SFPSKLIHT
Sbjct: 23  ETRNYIVHMNSAAMPKPFASRHSWYSATISSLLH----------SSSSSSSSFPSKLIHT 82

Query: 138 YNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISNC 197
           YNHAI+GF ASLTPS+LEALKNSPGY+SSVLDSSV VDTTHSS+FLGLSSN GLLPIS  
Sbjct: 83  YNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPISKY 142

Query: 198 GSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKG 257
           GSDVIIGFVDTGIWP+SESF DDGMSEIPS WKGECE+ THF+ S CN KLIGARFFNKG
Sbjct: 143 GSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKG 202

Query: 258 LSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYK 317
           L +  P  TIS+NSTRDTIGHGTHTSTTAAGSY++EASFFGYG+GTARGVAPRARVAIYK
Sbjct: 203 LISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYK 262

Query: 318 AIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATS 377
           AIWEEGNSVSDV+AAIDQAISDGVDVISLS+G+DGVPLY+DPVAIATFAAVERGIFVATS
Sbjct: 263 AIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATS 322

Query: 378 AGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLP 437
           AGNNGP+L TVHNGAPW+LNVAA TMDRDFGGTITLSNGVS+ GSSLFPLN T  +SPLP
Sbjct: 323 AGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSPLP 382

Query: 438 IVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLIK 497
           IVFMGGCQNLKKL+  GYKIVVCED DG+SL+SQVDNVQ++ VALGIFISNI DWDNLI+
Sbjct: 383 IVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIFISNIFDWDNLIQ 442

Query: 498 TPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPFV 557
           TPFPSIFL+P+HGNIIK YI KSSDPKAEV F+KTIL  KPAP VARYSSRGPS SCPFV
Sbjct: 443 TPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARYSSRGPSQSCPFV 502

Query: 558 LKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHRR 617
           LKPDIMAPGD ILASWPQNV AM+V S+PIY+KFNV+SGTSMSCPHAA VAALLKG H +
Sbjct: 503 LKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAAGVAALLKGAHPQ 562

Query: 618 WSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMGI 677
           WSPA IRSAMMTTAD++DNTQT IKD GN NKFATPLAMGSG VNPNKAIDPDLIYD+GI
Sbjct: 563 WSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNKAIDPDLIYDVGI 622

Query: 678 QDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGEF 737
           QDY+N+LCALNYT+NQI+ IT+S SN CENPSLDLNYPSFI+IVNSSDSKTRKR+I GEF
Sbjct: 623 QDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSDSKTRKRKISGEF 682

Query: 738 KRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETDV 797
           KRTLTKIGE RATYEAKL GMKG KVRVKP KL FKRKNQKLSF+LKIAGS R   E+++
Sbjct: 683 KRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKIAGSAR---ESNI 742

Query: 798 VYGYLSWAEVGGGHIVQSPIVVASL 823
           V+GYLSWAEVGGGHI+QSPIVV+ +
Sbjct: 743 VFGYLSWAEVGGGHIIQSPIVVSGM 754

BLAST of Clc08G17050 vs. NCBI nr
Match: KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1182.5 bits (3058), Expect = 0.0e+00
Identity = 603/747 (80.72%), Postives = 664/747 (88.89%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSV-LHTSFLDTTSSSSSSSSSLSFPSKLIH 137
           +TD YIVHM+ AAMPKPFA+ HSWYSAT+SSV L TS L TT+SSSSSS     PSKLIH
Sbjct: 25  QTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRTTTSSSSSS-----PSKLIH 84

Query: 138 TYNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISN 197
            Y HAINGF+A+LTPS+L+ALKNSPGYVSSV DSSVR DTTHSSNFL LS N GLLPISN
Sbjct: 85  CYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALSPNSGLLPISN 144

Query: 198 CGSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNK 257
            GSDVIIGFVDTG+WPESESFNDDG+ +IPS WKGECE+GTHF+ASLCNKKLIG RFFNK
Sbjct: 145 YGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKGECESGTHFNASLCNKKLIGGRFFNK 204

Query: 258 GLSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIY 317
           GL AKFPNVTISMNSTRDT GHGTHTSTTAAGSYV+EASFFGYGQGTARGVAPRARVAIY
Sbjct: 205 GLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVAPRARVAIY 264

Query: 318 KAIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVAT 377
           KAIW+EGN+VSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAA+ERGIFVAT
Sbjct: 265 KAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAMERGIFVAT 324

Query: 378 SAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPL 437
           SAGN GP+ GTVH+GAPWVLNVAA TMDRDFGGTITL+NGVS+ GSSLFPLN  + +SPL
Sbjct: 325 SAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLNLAIALSPL 384

Query: 438 PIVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLI 497
           PIVFMG C NLKKLK +G+KIVVCED D +SL  QVDNV+S+++A+G+FISNISDWDNLI
Sbjct: 385 PIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISNISDWDNLI 444

Query: 498 KTPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPF 557
           +T FPSIFL+ +HGN+IK YIK+SS+PKA VNF+KTI+G KPAPSVARYSSRGPS SCPF
Sbjct: 445 QTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSRGPSESCPF 504

Query: 558 VLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHR 617
           VLKPDIMAPGDAILASWPQNVAA +VYS PIY+K+NV+SGTSM+CPHAA VAALLKG H 
Sbjct: 505 VLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSGTSMACPHAAGVAALLKGAHP 564

Query: 618 RWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMG 677
            WSPA IRSAMMTTAD+VDNTQT IKD+GNKNK ATPLAMGSG VNPNKAIDP LIYDMG
Sbjct: 565 GWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAIDPGLIYDMG 624

Query: 678 IQDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGE 737
           I+DY NLLCALNYTKNQIQTIT+S SN CE P LDLNYPSFII VN+SDS+T + EI  E
Sbjct: 625 IEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPSFIITVNASDSQTGRTEISRE 684

Query: 738 FKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETD 797
           FKR +T IGEK ATY AK+  MKGL V V+P KL+FKRKNQ LSFKLKI G V VK E+D
Sbjct: 685 FKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGHVSVKRESD 744

Query: 798 VVYGYLSWAEVGGGHIVQSPIVVASLA 824
           VV+GYL+W EVGGGH VQSPIVVA +A
Sbjct: 745 VVFGYLNWVEVGGGHRVQSPIVVAVVA 766

BLAST of Clc08G17050 vs. NCBI nr
Match: XP_023546329.1 (subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 597/746 (80.03%), Postives = 663/746 (88.87%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHT 137
           ETD YIVHM+ AAMPKPFA+ HSWYSAT+SSVL    LDT+   +++SSS S PSKLIH 
Sbjct: 25  ETDNYIVHMDLAAMPKPFATHHSWYSATLSSVL----LDTSPLRTTTSSSSSLPSKLIHC 84

Query: 138 YNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISNC 197
           Y HAINGF+A+LTPS+L+ALKNSPGYVSSV DSSVR DTTHSSNFL LS N GLLPISN 
Sbjct: 85  YKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALSPNSGLLPISNY 144

Query: 198 GSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKG 257
           GSDVIIGFVDTG+WPESESFNDDG+S+IPS WKGECE+GTHF+ASLCNKKLIG RFFNKG
Sbjct: 145 GSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKLIGGRFFNKG 204

Query: 258 LSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYK 317
           L AKFPNVTISMNSTRDT GHGTHTSTTAAGSYV+EASFFGYGQGTARGVAPRARVAIYK
Sbjct: 205 LIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVAPRARVAIYK 264

Query: 318 AIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATS 377
           AIW+EGN VSDVIAAIDQAISDGVDVI+LSLG+D VPLYEDPVAIATFAA+ERGIFVATS
Sbjct: 265 AIWKEGNVVSDVIAAIDQAISDGVDVIALSLGIDRVPLYEDPVAIATFAAMERGIFVATS 324

Query: 378 AGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLP 437
           AGN GP+ GTVH+GAPWVLNVAA TMDRDFGGTITL+NGVS+ GSSLFPLNS + +SPLP
Sbjct: 325 AGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLNSAIALSPLP 384

Query: 438 IVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLIK 497
           I FMG C NLKKLK +G+KIVVCED D +SL  QVDNVQS+++A+G+FISNISDWDNLI+
Sbjct: 385 IFFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAVGVFISNISDWDNLIQ 444

Query: 498 TPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPFV 557
           T FPSIFL+ +HGN++K YI +SS+PKA VNF+KTI+G KPAPSVARYSSRGPS SCPFV
Sbjct: 445 TSFPSIFLNAYHGNVLKDYINRSSNPKARVNFHKTIIGTKPAPSVARYSSRGPSESCPFV 504

Query: 558 LKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHRR 617
           LKPDIMAPGDAILASWPQNVAA +VYS PIY+KFNV+SGTSM+CPHAA VAALLKG H  
Sbjct: 505 LKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVAALLKGAHPG 564

Query: 618 WSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMGI 677
           WSPA IRSAMMTTAD+VDNTQT IKD+GNKNK ATPLAMGSG VNPNKAIDP LIYDMGI
Sbjct: 565 WSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAIDPGLIYDMGI 624

Query: 678 QDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGEF 737
           +DY NL+CALNYTKNQIQTIT+S SN CE PSLDLNYPSFII VN+SDS+T + EI  EF
Sbjct: 625 EDYTNLICALNYTKNQIQTITRSTSNDCEKPSLDLNYPSFIITVNASDSETGRTEISREF 684

Query: 738 KRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETDV 797
           KR +T IGEK ATY AK+  MKGL V V+PKKL+FKRKNQ LSFKLKI G V VK E+DV
Sbjct: 685 KRRVTNIGEKGATYRAKMTPMKGLVVTVEPKKLKFKRKNQNLSFKLKIRGHVSVKRESDV 744

Query: 798 VYGYLSWAEVGGGHIVQSPIVVASLA 824
           V+GYL+W EVGGGH VQSPIVV +++
Sbjct: 745 VFGYLNWVEVGGGHRVQSPIVVTNMS 766

BLAST of Clc08G17050 vs. ExPASy Swiss-Prot
Match: O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 6.3e-178
Identity = 348/756 (46.03%), Postives = 486/756 (64.29%), Query Frame = 0

Query: 75  ATPETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKL 134
           A  +   YIVH++ + MP  F   H W+S+TI S+         +S  SS        KL
Sbjct: 20  ALAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSI--------KASVPSSVDRFHSAPKL 79

Query: 135 IHTYNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPI 194
           +++Y++ ++GFSA L+  EL ALK  PG++S+  D +V   TTH+S+FL L+ + GL P 
Sbjct: 80  VYSYDNVLHGFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTSDFLKLNPSSGLWPA 139

Query: 195 SNCGSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFF 254
           S  G DVI+  +D+GIWPES SF DDGM EIP  WKG C+ GT F+AS+CN+KLIGA +F
Sbjct: 140 SGLGQDVIVAVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFNASMCNRKLIGANYF 199

Query: 255 NKGLSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVA 314
           NKG+ A  P V I+MNS RDT GHGTH ++  AG++ +  S FGY  GTARGVAPRAR+A
Sbjct: 200 NKGILANDPTVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYAPGTARGVAPRARLA 259

Query: 315 IYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFV 374
           +YK  + EG   SD+IAA+DQA++DGVD+IS+S G   +PLYED ++IA+F A+ +G+ V
Sbjct: 260 VYKFSFNEGTFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAISIASFGAMMKGVLV 319

Query: 375 ATSAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVS 434
           + SAGN GP +G+++NG+PW+L VA+   DR F GT+TL NG+ I G SLFP  +   V 
Sbjct: 320 SASAGNRGPGIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIRGWSLFPARAF--VR 379

Query: 435 PLPIVF---MGGCQN---LKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISN 494
             P+++   +  C +   L ++++    IV+C+D      S Q+  +  +R+   IFIS 
Sbjct: 380 DSPVIYNKTLSDCSSEELLSQVENPENTIVICDDNG--DFSDQMRIITRARLKAAIFISE 439

Query: 495 ISDWDNLIKTPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSR 554
                     P P + ++   G  +  Y+K S  P A + F +T L  KPAP VA  S+R
Sbjct: 440 DPGVFRSATFPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKPAPVVAASSAR 499

Query: 555 GPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIY-TKFNVMSGTSMSCPHAAAV 614
           GPS S   + KPDI+APG  ILA++P NV A ++ ++ +  T + + SGTSM+ PHAA +
Sbjct: 500 GPSRSYLGISKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGTSMAAPHAAGI 559

Query: 615 AALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAI 674
           AA+LK  H  WSP+ IRSAMMTTAD +DNT+  IKD  N NK ATPL MG+G V+PN+A+
Sbjct: 560 AAMLKAAHPEWSPSAIRSAMMTTADPLDNTRKPIKDSDN-NKAATPLDMGAGHVDPNRAL 619

Query: 675 DPDLIYDMGIQDYINLLCALNYTKNQIQTITQ-SASNKCENPSLDLNYPSFIIIVNSSDS 734
           DP L+YD   QDY+NLLC+LN+T+ Q +TI + SAS+ C NPS DLNYPSFI + +   +
Sbjct: 620 DPGLVYDATPQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPSFIALYSIEGN 679

Query: 735 KTRKREILGEFKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIA 794
            T   +   +FKRT+T +G+  ATY+AKL+  K   + V P+ L FK KN+K S+ L I 
Sbjct: 680 FTLLEQ---KFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNEKQSYTLTIR 739

Query: 795 GSVRVKTETDVVYGYLSWAEVGGGHIVQSPIVVASL 823
                    +V  G ++W E  G H V+SPIV + +
Sbjct: 740 YIGDEGQSRNV--GSITWVEQNGNHSVRSPIVTSPI 757

BLAST of Clc08G17050 vs. ExPASy Swiss-Prot
Match: Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 2.6e-163
Identity = 334/762 (43.83%), Postives = 466/762 (61.15%), Query Frame = 0

Query: 76  TPETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLI 135
           T ET  YI+HM+ +A P PF+   SW+S T++SV+                  +   K+I
Sbjct: 19  TAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI-----------------TNRKPKII 78

Query: 136 HTYNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPIS 195
           + Y  +++GFSA LT SEL+ LK+ PGYVS   D  V++ TT S  F+GL+S  G  P+S
Sbjct: 79  YAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPVS 138

Query: 196 NCGSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFN 255
           N G+ ++IG +DTGIWP+S SF+DDG+  +PS WKG CE     S+SLCNKKLIGA+ FN
Sbjct: 139 NYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---SSSLCNKKLIGAKVFN 198

Query: 256 KGLSAKFPNVTIS----MNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRA 315
           KGL A  P++  +     +S  DTIGHGTH +  AAG++V+ AS+F Y QGTA G+AP A
Sbjct: 199 KGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPHA 258

Query: 316 RVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSLGL--------DGVPLYEDPVAIA 375
            +AIYKA WEEG   SDVIAAIDQAI DGV VISLSLGL        DG  L  DP+A+A
Sbjct: 259 HLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAVA 318

Query: 376 TFAAVERGIFVATSAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSS 435
           +FAA+++G+FV TS GN+GP   ++ NGAPW++ V A T+ R F GT+T  N VS    S
Sbjct: 319 SFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFPS 378

Query: 436 LFPLNSTMDVSPLPIVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALG 495
           LFP        P+  +  G  +N    K++  +IVVC   +  ++ S++  ++S+  A  
Sbjct: 379 LFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGAAAV 438

Query: 496 IFISN-ISDWDNLIKTPFPSIFLSPHHGNIIKGYIKKS-SDPKAEVNFNKTILGIKPAPS 555
           + I++ + +  + IK  FP  F+   H   I+ Y   + ++  A++ F KT++G KPAP 
Sbjct: 439 VLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKPAPE 498

Query: 556 VARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSC 615
           V  YSSRGP  S P +LKPDI+APG  IL++WP         + P+++ FN+++GTSM+ 
Sbjct: 499 VGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTSMAA 558

Query: 616 PHAAAVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQV 675
           PH A VAAL+K VH  WSP+ I+SA+MTTA  +DN                PLA+G+G V
Sbjct: 559 PHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGAGHV 618

Query: 676 NPNKAIDPDLIYDMGIQDYINLLC-ALNYTKNQIQTITQS-ASNKCENPSLDLNYPSFII 735
           + NK ++P LIYD   QD+IN LC     ++  I  IT+S  S+ C+ PS  LNYPS I 
Sbjct: 619 STNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPSIIA 678

Query: 736 IVNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKL 795
              S  S  +       FKRTLT +GE + +Y  ++RG+KGL V V+PKKL F  KN+KL
Sbjct: 679 YFTSDQSSPK------IFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNEKL 730

Query: 796 SFKLKIAGSVRVKTETDVVYGYLSWAEVGGGHIVQSPIVVAS 822
           S+ +++     +  + +VVYG +SW +        S  VVA+
Sbjct: 739 SYTVRLESPRGL--QENVVYGLVSWVDEDEAEFEVSCSVVAT 730

BLAST of Clc08G17050 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 7.3e-142
Identity = 302/757 (39.89%), Postives = 427/757 (56.41%), Query Frame = 0

Query: 82  YIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHTYNHA 141
           YI+ +N +  P+ F + H WY                      +S L+  S L++TY  +
Sbjct: 30  YIIRVNHSDKPESFLTHHDWY----------------------TSQLNSESSLLYTYTTS 89

Query: 142 INGFSASLTPSELEALKNSPGYVSSVL-DSSVRVDTTHSSNFLGLSSNFGLLPISNCGSD 201
            +GFSA L  +E ++L +S   +  +  D    + TT +  FLGL+S FG+  + +  + 
Sbjct: 90  FHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNG 149

Query: 202 VIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKGL-- 261
           VIIG +DTG+WPES SF+D  M EIPS WKGECE+G+ F + LCNKKLIGAR F+KG   
Sbjct: 150 VIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQM 209

Query: 262 --SAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIY 321
                F +   S+ S RD  GHGTHTSTTAAGS V  ASF GY  GTARG+A RARVA Y
Sbjct: 210 ASGGGFSSKRESV-SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATY 269

Query: 322 KAIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVAT 381
           K  W  G   SD++AA+D+AI DGVDV+SLSLG    P Y D +AI  F+A+ERG+FV+ 
Sbjct: 270 KVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSC 329

Query: 382 SAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPL 441
           SAGN+GP   +V N APWV+ V A T+DRDF     L NG  + G SL+     M   PL
Sbjct: 330 SAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKPL 389

Query: 442 PIVFMGGCQNLKKL--------KSIGYKIVVCEDKDGHSLSSQVDNVQSSRVA--LGIFI 501
            +V+  G  +   L          +  KIVVC+      ++++V+     R A  LG+ +
Sbjct: 390 ELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCD----RGVNARVEKGAVVRDAGGLGMIM 449

Query: 502 SN-ISDWDNLIKTP--FPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVA 561
           +N  +  + L+      P+I +    G++++ Y+K  S P A + F  T+L +KP+P VA
Sbjct: 450 ANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVA 509

Query: 562 RYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPH 621
            +SSRGP+   P +LKPD++ PG  ILA W   +    +      T+FN+MSGTSMSCPH
Sbjct: 510 AFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPH 569

Query: 622 AAAVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNP 681
            + +A LLK  H  WSP+ I+SA+MTTA V+DNT   + D  + N  + P A GSG V+P
Sbjct: 570 ISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVDP 629

Query: 682 NKAIDPDLIYDMGIQDYINLLCALNYTKNQIQTITQSASNKCENPSLD---LNYPSFIII 741
            KA+ P L+YD+  ++YI  LC+L+YT + I  I +  S  C     D   LNYPSF ++
Sbjct: 630 QKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVL 689

Query: 742 VNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLS 801
                    KR +   + R +T +G   + Y+  + G   + + VKP KL FK   +K  
Sbjct: 690 FGG------KRVV--RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKR 747

Query: 802 FKLKIAGSVRVKTETDVVYGYLSWAEVGGGHIVQSPI 818
           + +       V       +G ++W+     H V+SP+
Sbjct: 750 YTVTFVSKKGVSMTNKAEFGSITWS--NPQHEVRSPV 747

BLAST of Clc08G17050 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 1.7e-138
Identity = 307/761 (40.34%), Postives = 433/761 (56.90%), Query Frame = 0

Query: 82  YIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHTYNHA 141
           YIVHM  + MP  F    +WY                    SS  S+S  ++L++TY +A
Sbjct: 32  YIVHMAKSQMPSSFDLHSNWY-------------------DSSLRSISDSAELLYTYENA 91

Query: 142 INGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFG-LLPISNCGSD 201
           I+GFS  LT  E ++L   PG +S + +    + TT +  FLGL  +   L P +   SD
Sbjct: 92  IHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSD 151

Query: 202 VIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKGLSA 261
           V++G +DTG+WPES+S++D+G   IPSSWKG CE GT+F+ASLCN+KLIGARFF +G  +
Sbjct: 152 VVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYES 211

Query: 262 KFPNVTIS--MNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYKA 321
               +  S    S RD  GHGTHTS+TAAGS VE AS  GY  GTARG+APRARVA+YK 
Sbjct: 212 TMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKV 271

Query: 322 IWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATSA 381
            W  G   SD++AAID+AI+D V+V+S+SLG      Y D VAI  FAA+ERGI V+ SA
Sbjct: 272 CWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSA 331

Query: 382 GNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLPI 441
           GN GP   ++ N APW+  V A T+DRDF     L NG +  G SLF   +  D   LP 
Sbjct: 332 GNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLPF 391

Query: 442 VFMGGCQNLKK----------LKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISN 501
           ++ G   N              + +  KIV+C+   G +   Q  +V  +   +G+ ++N
Sbjct: 392 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILAN 451

Query: 502 -ISDWDNLIKTP--FPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARY 561
             ++ + L+      P+  +    G+II+ Y+    +P A ++   T++G+KP+P VA +
Sbjct: 452 TAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAF 511

Query: 562 SSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAA 621
           SSRGP+   P +LKPD++APG  ILA+W        + S     +FN++SGTSMSCPH +
Sbjct: 512 SSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVS 571

Query: 622 AVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNK 681
            +AALLK VH  WSPA IRSA+MTTA         + DI    K +TP   G+G V+P  
Sbjct: 572 GLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT-GKPSTPFDHGAGHVSPTT 631

Query: 682 AIDPDLIYDMGIQDYINLLCALNYTKNQIQTITQSASNKCENPS-----LDLNYPSFIII 741
           A +P LIYD+  +DY+  LCALNYT  QI+++  S  N   +PS      DLNYPSF + 
Sbjct: 632 ATNPGLIYDLTTEDYLGFLCALNYTSPQIRSV--SRRNYTCDPSKSYSVADLNYPSFAVN 691

Query: 742 VNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRG-MKGLKVRVKPKKLEFKRKNQKL 801
           V+   +         ++ RT+T +G    TY  K+     G+K+ V+P  L FK  N+K 
Sbjct: 692 VDGVGAY--------KYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 751

Query: 802 SFKLKIAGSVRVKTETDVVYGYLSWAEVGGGHIVQSPIVVA 821
           S+ +        K      +G + W++  G H+V SP+ ++
Sbjct: 752 SYTVTFTVD-SSKPSGSNSFGSIEWSD--GKHVVGSPVAIS 755

BLAST of Clc08G17050 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 2.2e-138
Identity = 302/775 (38.97%), Postives = 440/775 (56.77%), Query Frame = 0

Query: 75  ATPETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKL 134
           A+     +I  ++  +MP  F + + WYS        T F +               S++
Sbjct: 21  ASQAAKTFIFRIDGGSMPSIFPTHYHWYS--------TEFAE--------------ESRI 80

Query: 135 IHTYNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPI 194
           +H Y+   +GFSA +TP E + L+N P  ++   D    + TT S  FLGL +  GL   
Sbjct: 81  VHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSE 140

Query: 195 SNCGSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFF 254
           S+ GSDVIIG  DTGIWPE  SF+D  +  IP  W+G CE+G  FS   CN+K+IGARFF
Sbjct: 141 SDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFF 200

Query: 255 NKGLSAKF---PNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRA 314
            KG  A      N T+   S RD  GHGTHTS+TAAG +  +AS  GY  G A+GVAP+A
Sbjct: 201 AKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKA 260

Query: 315 RVAIYKAIWEEGNSV-SDVIAAIDQAISDGVDVISLSL-GLDGV--PLYEDPVAIATFAA 374
           R+A YK  W++   + SD++AA D A+ DGVDVIS+S+ G DG+  P Y DP+AI ++ A
Sbjct: 261 RIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGA 320

Query: 375 VERGIFVATSAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLF-- 434
             +GIFV++SAGN GP   +V N APWV  V A+T+DR+F     L +G  + G SL+  
Sbjct: 321 ASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAG 380

Query: 435 -PLNSTMDVSPLPIVFMGG---------CQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNV 494
            PLN  M     P+V+ G           +N    K +  KIV+C+      ++  +   
Sbjct: 381 VPLNGRM----FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 440

Query: 495 QSSRVALGIFISNISDWDNLIKTP--FPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTI 554
           ++  V + I  +  S+ + L+      P+  +  + G+ IK Y     +P A ++F  TI
Sbjct: 441 KAGGVGM-ILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTI 500

Query: 555 LGIKPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNV 614
           +GIKPAP +A +S RGP+   P +LKPD++APG  ILA+W   V    + S P  T+FN+
Sbjct: 501 VGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNI 560

Query: 615 MSGTSMSCPHAAAVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATP 674
           +SGTSM+CPH +  AALLK  H  WSPA IRSAMMTT ++VDN+  S+ D  +  K ATP
Sbjct: 561 LSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATP 620

Query: 675 LAMGSGQVNPNKAIDPDLIYDMGIQDYINLLCALNYTKNQIQTITQS----ASNKCENPS 734
              GSG +N  +A++P L+YD+   DYI  LC++ Y    IQ IT++     + +  +P 
Sbjct: 621 YDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPG 680

Query: 735 LDLNYPSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKK 794
            +LNYPS   +  ++      + ++    RT T +G+  A Y A++   +G+ V VKP +
Sbjct: 681 -NLNYPSITAVFPTNRRGLVSKTVI----RTATNVGQAEAVYRARIESPRGVTVTVKPPR 740

Query: 795 LEFKRKNQKLSFKLKIAGSVR--VKTETDVVYGYLSWAEVGGGHIVQSPIVVASL 823
           L F    ++ S+ + +  + R  V  ET  V+G ++W + GG H+V+SPIVV  +
Sbjct: 741 LVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVVTQM 761

BLAST of Clc08G17050 vs. ExPASy TrEMBL
Match: A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)

HSP 1 Score: 1258.8 bits (3256), Expect = 0.0e+00
Identity = 638/745 (85.64%), Postives = 691/745 (92.75%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHT 137
           ET  YIVHMNSAAMPKPFASRHSWYSATISSVLH+S   ++SSSSSSSSS SFPSKLIHT
Sbjct: 23  ETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSS---SSSSSSSSSSSSSFPSKLIHT 82

Query: 138 YNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISNC 197
           YNHAI+GF ASLTPS+LEALKNSPGY+SSVLDSSV VDTTHSS+FLGLSSN GLLPIS  
Sbjct: 83  YNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPISKY 142

Query: 198 GSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKG 257
           GSDVIIGFVDTGIWP+SESFNDDGMSEIPS WKGECE+ THF+ S CN KLIGARFFNKG
Sbjct: 143 GSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGARFFNKG 202

Query: 258 LSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYK 317
           L A+FPN TISMNSTRDTIGHGTHTST AAGSYV+EASFFGYG+GTARGVAPRARVAIYK
Sbjct: 203 LIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGTARGVAPRARVAIYK 262

Query: 318 AIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATS 377
           AIW+EGNSVSDV+AAIDQAISDGVDVISLS+G+DG+PLY DPV+IATFAAVERGIFVATS
Sbjct: 263 AIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIATFAAVERGIFVATS 322

Query: 378 AGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLP 437
           AGNNGP+LGTVHNGAPWVLNVAA TMDRDFGGTITLSNGVS+ GSSLFPLN+TM +SPLP
Sbjct: 323 AGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTMGLSPLP 382

Query: 438 IVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLIK 497
           IVFMGGCQNLKKLK IGYKIVVCED DG+SL+SQVDNVQ+++V LGIFISNI DWDNLI+
Sbjct: 383 IVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTLGIFISNIFDWDNLIQ 442

Query: 498 TPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPFV 557
           TPFPSIF++P+HGNIIK YI KSSDPKAEVNF+KTILG KPAP+VARYSSRGPS SCPFV
Sbjct: 443 TPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTVARYSSRGPSQSCPFV 502

Query: 558 LKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHRR 617
           LKPDIMAPGDAILASWPQN+ AM+V S+PIYTKFNV+SGTSMSCPHAA VAALLKG H +
Sbjct: 503 LKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALLKGAHPQ 562

Query: 618 WSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMGI 677
           WSPA IRSAMMTTADVVDNTQTSIKDIGN NKFATPLAMGSG VNPNKA+DPDLIYD+GI
Sbjct: 563 WSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDLIYDVGI 622

Query: 678 QDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGEF 737
           QDY+N+LCALNYT+NQI+ IT+S SN CENPSLDLNYPSFIII NSSDSKT KR+ILGEF
Sbjct: 623 QDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIANSSDSKTGKRKILGEF 682

Query: 738 KRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETDV 797
           KRTLTKIGE RATYEAKLRGMKG KVRVKP+ LEFKRKNQKLSF+LKIAGS     E++V
Sbjct: 683 KRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAGSAE---ESNV 742

Query: 798 VYGYLSWAEVGGGHIVQSPIVVASL 823
           V+GYLSWAEVGG HIVQSPIVVA +
Sbjct: 743 VFGYLSWAEVGGRHIVQSPIVVAGM 761

BLAST of Clc08G17050 vs. ExPASy TrEMBL
Match: A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)

HSP 1 Score: 1258.8 bits (3256), Expect = 0.0e+00
Identity = 638/745 (85.64%), Postives = 691/745 (92.75%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHT 137
           ET  YIVHMNSAAMPKPFASRHSWYSATISSVLH+S   ++SSSSSSSSS SFPSKLIHT
Sbjct: 23  ETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSS---SSSSSSSSSSSSSFPSKLIHT 82

Query: 138 YNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISNC 197
           YNHAI+GF ASLTPS+LEALKNSPGY+SSVLDSSV VDTTHSS+FLGLSSN GLLPIS  
Sbjct: 83  YNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPISKY 142

Query: 198 GSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKG 257
           GSDVIIGFVDTGIWP+SESFNDDGMSEIPS WKGECE+ THF+ S CN KLIGARFFNKG
Sbjct: 143 GSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGARFFNKG 202

Query: 258 LSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYK 317
           L A+FPN TISMNSTRDTIGHGTHTST AAGSYV+EASFFGYG+GTARGVAPRARVAIYK
Sbjct: 203 LIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGTARGVAPRARVAIYK 262

Query: 318 AIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATS 377
           AIW+EGNSVSDV+AAIDQAISDGVDVISLS+G+DG+PLY DPV+IATFAAVERGIFVATS
Sbjct: 263 AIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIATFAAVERGIFVATS 322

Query: 378 AGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLP 437
           AGNNGP+LGTVHNGAPWVLNVAA TMDRDFGGTITLSNGVS+ GSSLFPLN+TM +SPLP
Sbjct: 323 AGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTMGLSPLP 382

Query: 438 IVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLIK 497
           IVFMGGCQNLKKLK IGYKIVVCED DG+SL+SQVDNVQ+++V LGIFISNI DWDNLI+
Sbjct: 383 IVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTLGIFISNIFDWDNLIQ 442

Query: 498 TPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPFV 557
           TPFPSIF++P+HGNIIK YI KSSDPKAEVNF+KTILG KPAP+VARYSSRGPS SCPFV
Sbjct: 443 TPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTVARYSSRGPSQSCPFV 502

Query: 558 LKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHRR 617
           LKPDIMAPGDAILASWPQN+ AM+V S+PIYTKFNV+SGTSMSCPHAA VAALLKG H +
Sbjct: 503 LKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALLKGAHPQ 562

Query: 618 WSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMGI 677
           WSPA IRSAMMTTADVVDNTQTSIKDIGN NKFATPLAMGSG VNPNKA+DPDLIYD+GI
Sbjct: 563 WSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDLIYDVGI 622

Query: 678 QDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGEF 737
           QDY+N+LCALNYT+NQI+ IT+S SN CENPSLDLNYPSFIII NSSDSKT KR+ILGEF
Sbjct: 623 QDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIANSSDSKTGKRKILGEF 682

Query: 738 KRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETDV 797
           KRTLTKIGE RATYEAKLRGMKG KVRVKP+ LEFKRKNQKLSF+LKIAGS     E++V
Sbjct: 683 KRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAGSAE---ESNV 742

Query: 798 VYGYLSWAEVGGGHIVQSPIVVASL 823
           V+GYLSWAEVGG HIVQSPIVVA +
Sbjct: 743 VFGYLSWAEVGGRHIVQSPIVVAGM 761

BLAST of Clc08G17050 vs. ExPASy TrEMBL
Match: A0A0A0LKL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354760 PE=3 SV=1)

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 620/745 (83.22%), Postives = 673/745 (90.34%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHT 137
           ET  YIVHMNSAAMPKPFASRHSWYSATISS+LH          SSSSSS SFPSKLIHT
Sbjct: 23  ETRNYIVHMNSAAMPKPFASRHSWYSATISSLLH----------SSSSSSSSFPSKLIHT 82

Query: 138 YNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISNC 197
           YNHAI+GF ASLTPS+LEALKNSPGY+SSVLDSSV VDTTHSS+FLGLSSN GLLPIS  
Sbjct: 83  YNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPISKY 142

Query: 198 GSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKG 257
           GSDVIIGFVDTGIWP+SESF DDGMSEIPS WKGECE+ THF+ S CN KLIGARFFNKG
Sbjct: 143 GSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCNNKLIGARFFNKG 202

Query: 258 LSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYK 317
           L +  P  TIS+NSTRDTIGHGTHTSTTAAGSY++EASFFGYG+GTARGVAPRARVAIYK
Sbjct: 203 LISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTARGVAPRARVAIYK 262

Query: 318 AIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATS 377
           AIWEEGNSVSDV+AAIDQAISDGVDVISLS+G+DGVPLY+DPVAIATFAAVERGIFVATS
Sbjct: 263 AIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATFAAVERGIFVATS 322

Query: 378 AGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLP 437
           AGNNGP+L TVHNGAPW+LNVAA TMDRDFGGTITLSNGVS+ GSSLFPLN T  +SPLP
Sbjct: 323 AGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNITTGLSPLP 382

Query: 438 IVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLIK 497
           IVFMGGCQNLKKL+  GYKIVVCED DG+SL+SQVDNVQ++ VALGIFISNI DWDNLI+
Sbjct: 383 IVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIFISNIFDWDNLIQ 442

Query: 498 TPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPFV 557
           TPFPSIFL+P+HGNIIK YI KSSDPKAEV F+KTIL  KPAP VARYSSRGPS SCPFV
Sbjct: 443 TPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARYSSRGPSQSCPFV 502

Query: 558 LKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHRR 617
           LKPDIMAPGD ILASWPQNV AM+V S+PIY+KFNV+SGTSMSCPHAA VAALLKG H +
Sbjct: 503 LKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAAGVAALLKGAHPQ 562

Query: 618 WSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMGI 677
           WSPA IRSAMMTTAD++DNTQT IKD GN NKFATPLAMGSG VNPNKAIDPDLIYD+GI
Sbjct: 563 WSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNKAIDPDLIYDVGI 622

Query: 678 QDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGEF 737
           QDY+N+LCALNYT+NQI+ IT+S SN CENPSLDLNYPSFI+IVNSSDSKTRKR+I GEF
Sbjct: 623 QDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSDSKTRKRKISGEF 682

Query: 738 KRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETDV 797
           KRTLTKIGE RATYEAKL GMKG KVRVKP KL FKRKNQKLSF+LKIAGS R   E+++
Sbjct: 683 KRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKIAGSAR---ESNI 742

Query: 798 VYGYLSWAEVGGGHIVQSPIVVASL 823
           V+GYLSWAEVGGGHI+QSPIVV+ +
Sbjct: 743 VFGYLSWAEVGGGHIIQSPIVVSGM 754

BLAST of Clc08G17050 vs. ExPASy TrEMBL
Match: A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 601/747 (80.46%), Postives = 665/747 (89.02%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSV-LHTSFLDTTSSSSSSSSSLSFPSKLIH 137
           +TD YIVHM+ AAMPKPFA+ HSWYSAT+SSV L TS L TT+SSSSS    S PSKLIH
Sbjct: 25  QTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRTTTSSSSS----SLPSKLIH 84

Query: 138 TYNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISN 197
            Y HAINGF+ASLTPS+L+ALKNSPGYVSS+ DSSVR DTTHSSNFL LS N GLLPISN
Sbjct: 85  CYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSNFLALSPNSGLLPISN 144

Query: 198 CGSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNK 257
            GSDVIIGFVDTG+WPESESFNDDG+S+IPS WKGECE+GTHF+ASLCNKKLIG RFFNK
Sbjct: 145 YGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKLIGGRFFNK 204

Query: 258 GLSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIY 317
           GL +KFPNVTISMNSTRDT GHGTHTSTTAAGSYV+EASFFGYGQGTARGVAPRARVAIY
Sbjct: 205 GLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVAPRARVAIY 264

Query: 318 KAIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVAT 377
           KAIW+EGN+VSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAA+ERGIFVAT
Sbjct: 265 KAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAMERGIFVAT 324

Query: 378 SAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPL 437
           SAGN GP+ GTVH+GAPWVLNVAA TMDRDFGGTITL+NGVS+ GSSLFPLNS + +SPL
Sbjct: 325 SAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLNSAIALSPL 384

Query: 438 PIVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLI 497
           PIVFMG C NLKKLK +G+KIVVCED D +SL  QVDNV+S+++A+G+FISNISDWDNLI
Sbjct: 385 PIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISNISDWDNLI 444

Query: 498 KTPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPF 557
           +T FPSIFL+ +HGN+IK YIK+SS+PKA VNF+KTI+G KPAPSVARYSSRGPS SCPF
Sbjct: 445 QTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSRGPSESCPF 504

Query: 558 VLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHR 617
           VLKPDIMAPGDAILASWPQNVAA +VYS PIY+KFNV+SGTSM+CPHAA VAALLKG H 
Sbjct: 505 VLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVAALLKGAHP 564

Query: 618 RWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMG 677
            WSPA IRSAMMTTAD+VDNTQT IKD+GNKNK ATPLAMGSG VNPNKAIDP LIYDMG
Sbjct: 565 GWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAIDPGLIYDMG 624

Query: 678 IQDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGE 737
           I+DY NLLCALNYTKNQIQTIT+S SN CE   LDLNYPSFI+ VN+SDS+T + E+  E
Sbjct: 625 IEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTVNASDSQTGRTEMSRE 684

Query: 738 FKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETD 797
           FKR +T IGEK ATY AK+  MKGL V V+P KL+FKRKNQ LSFKLKI G V +K E+D
Sbjct: 685 FKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGHVSIKRESD 744

Query: 798 VVYGYLSWAEVGGGHIVQSPIVVASLA 824
           VV+GYL+W EVGGGH VQSPIVVA +A
Sbjct: 745 VVFGYLNWVEVGGGHRVQSPIVVAVVA 767

BLAST of Clc08G17050 vs. ExPASy TrEMBL
Match: A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 598/745 (80.27%), Postives = 660/745 (88.59%), Query Frame = 0

Query: 78  ETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHT 137
           ETD YIVHM+ AAMPKPFA+ HSWYSAT+SSVL    LDT+   +++SSS S PSKLIH 
Sbjct: 25  ETDNYIVHMDLAAMPKPFATHHSWYSATLSSVL----LDTSPLRTTTSSSSSLPSKLIHC 84

Query: 138 YNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPISNC 197
           Y HAINGF+A+LTPS+L+ALKNSPGYVSSV DSSVR DTTHSSNFL L+ N GLLPISN 
Sbjct: 85  YKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALTPNSGLLPISNY 144

Query: 198 GSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKG 257
           GSDVIIGFVDTG+WPESESFND+ +S+IPS WKGECE+GTHF+ASLCNKKLIG RFFNKG
Sbjct: 145 GSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFNASLCNKKLIGGRFFNKG 204

Query: 258 LSAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYK 317
           L AKFPNVTISMNSTRDT GHGTHTSTTAAGSYV+EASFFGYGQGTARGVAPRARVAIYK
Sbjct: 205 LIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVAPRARVAIYK 264

Query: 318 AIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATS 377
           AIW+EGN VSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAA+ERGIFVATS
Sbjct: 265 AIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAMERGIFVATS 324

Query: 378 AGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLP 437
           AGN GP+ GTVH+GAPWVLNVAA TMDRDFGGTITL+NGVS+ GSSLF LNS + +SPLP
Sbjct: 325 AGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFVLNSAIVLSPLP 384

Query: 438 IVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISNISDWDNLIK 497
           IVFMG C NLKKLK +G+KIVVCED D +SL  QVDNVQS+++A+G+FISNISDWDNLI+
Sbjct: 385 IVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAVGVFISNISDWDNLIQ 444

Query: 498 TPFPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARYSSRGPSMSCPFV 557
           T FPSIFL+ +HGN+IK YI +SS+PKA VNF+KTI+G KPAPSVARYSSRGPS SCP V
Sbjct: 445 TSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAPSVARYSSRGPSESCPLV 504

Query: 558 LKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHRR 617
           LKPDIMAPGDAILASWPQ VAA +VYS PIY+KFNV+SGTSM+CPHAA VAALLKG H  
Sbjct: 505 LKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMACPHAAGVAALLKGAHPG 564

Query: 618 WSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMGI 677
           WSPA IRSAMMTTADVVDNTQT IKD+GNKNK ATPLAMGSG VNPNKAIDP LIYD+GI
Sbjct: 565 WSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAIDPGLIYDIGI 624

Query: 678 QDYINLLCALNYTKNQIQTITQSASNKCENPSLDLNYPSFIIIVNSSDSKTRKREILGEF 737
           +DY NLLCALNYTKNQIQTIT+S SN CE PSLDLNYPSFII VN+SDS++ +REI  EF
Sbjct: 625 EDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIITVNASDSESGRREISREF 684

Query: 738 KRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTETDV 797
           KR +T IGEK ATY AK+  MKGL V V+PK L+FKRKNQ LSFKLKI G V VK E+DV
Sbjct: 685 KRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLSFKLKIRGHVSVKRESDV 744

Query: 798 VYGYLSWAEVGGGHIVQSPIVVASL 823
           V+GYL+W EVGGGH VQSPIVVA L
Sbjct: 745 VFGYLNWVEVGGGHRVQSPIVVAGL 765

BLAST of Clc08G17050 vs. TAIR 10
Match: AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 577.4 bits (1487), Expect = 1.8e-164
Identity = 334/762 (43.83%), Postives = 466/762 (61.15%), Query Frame = 0

Query: 76  TPETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLI 135
           T ET  YI+HM+ +A P PF+   SW+S T++SV+                  +   K+I
Sbjct: 19  TAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI-----------------TNRKPKII 78

Query: 136 HTYNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPIS 195
           + Y  +++GFSA LT SEL+ LK+ PGYVS   D  V++ TT S  F+GL+S  G  P+S
Sbjct: 79  YAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPVS 138

Query: 196 NCGSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFN 255
           N G+ ++IG +DTGIWP+S SF+DDG+  +PS WKG CE     S+SLCNKKLIGA+ FN
Sbjct: 139 NYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---SSSLCNKKLIGAKVFN 198

Query: 256 KGLSAKFPNVTIS----MNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRA 315
           KGL A  P++  +     +S  DTIGHGTH +  AAG++V+ AS+F Y QGTA G+AP A
Sbjct: 199 KGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPHA 258

Query: 316 RVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSLGL--------DGVPLYEDPVAIA 375
            +AIYKA WEEG   SDVIAAIDQAI DGV VISLSLGL        DG  L  DP+A+A
Sbjct: 259 HLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAVA 318

Query: 376 TFAAVERGIFVATSAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSS 435
           +FAA+++G+FV TS GN+GP   ++ NGAPW++ V A T+ R F GT+T  N VS    S
Sbjct: 319 SFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFPS 378

Query: 436 LFPLNSTMDVSPLPIVFMGGCQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALG 495
           LFP        P+  +  G  +N    K++  +IVVC   +  ++ S++  ++S+  A  
Sbjct: 379 LFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGAAAV 438

Query: 496 IFISN-ISDWDNLIKTPFPSIFLSPHHGNIIKGYIKKS-SDPKAEVNFNKTILGIKPAPS 555
           + I++ + +  + IK  FP  F+   H   I+ Y   + ++  A++ F KT++G KPAP 
Sbjct: 439 VLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKPAPE 498

Query: 556 VARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSC 615
           V  YSSRGP  S P +LKPDI+APG  IL++WP         + P+++ FN+++GTSM+ 
Sbjct: 499 VGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTSMAA 558

Query: 616 PHAAAVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQV 675
           PH A VAAL+K VH  WSP+ I+SA+MTTA  +DN                PLA+G+G V
Sbjct: 559 PHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGAGHV 618

Query: 676 NPNKAIDPDLIYDMGIQDYINLLC-ALNYTKNQIQTITQS-ASNKCENPSLDLNYPSFII 735
           + NK ++P LIYD   QD+IN LC     ++  I  IT+S  S+ C+ PS  LNYPS I 
Sbjct: 619 STNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPSIIA 678

Query: 736 IVNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKL 795
              S  S  +       FKRTLT +GE + +Y  ++RG+KGL V V+PKKL F  KN+KL
Sbjct: 679 YFTSDQSSPK------IFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNEKL 730

Query: 796 SFKLKIAGSVRVKTETDVVYGYLSWAEVGGGHIVQSPIVVAS 822
           S+ +++     +  + +VVYG +SW +        S  VVA+
Sbjct: 739 SYTVRLESPRGL--QENVVYGLVSWVDEDEAEFEVSCSVVAT 730

BLAST of Clc08G17050 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 506.1 bits (1302), Expect = 5.2e-143
Identity = 302/757 (39.89%), Postives = 427/757 (56.41%), Query Frame = 0

Query: 82  YIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHTYNHA 141
           YI+ +N +  P+ F + H WY                      +S L+  S L++TY  +
Sbjct: 30  YIIRVNHSDKPESFLTHHDWY----------------------TSQLNSESSLLYTYTTS 89

Query: 142 INGFSASLTPSELEALKNSPGYVSSVL-DSSVRVDTTHSSNFLGLSSNFGLLPISNCGSD 201
            +GFSA L  +E ++L +S   +  +  D    + TT +  FLGL+S FG+  + +  + 
Sbjct: 90  FHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNG 149

Query: 202 VIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKGL-- 261
           VIIG +DTG+WPES SF+D  M EIPS WKGECE+G+ F + LCNKKLIGAR F+KG   
Sbjct: 150 VIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQM 209

Query: 262 --SAKFPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIY 321
                F +   S+ S RD  GHGTHTSTTAAGS V  ASF GY  GTARG+A RARVA Y
Sbjct: 210 ASGGGFSSKRESV-SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATY 269

Query: 322 KAIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVAT 381
           K  W  G   SD++AA+D+AI DGVDV+SLSLG    P Y D +AI  F+A+ERG+FV+ 
Sbjct: 270 KVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSC 329

Query: 382 SAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPL 441
           SAGN+GP   +V N APWV+ V A T+DRDF     L NG  + G SL+     M   PL
Sbjct: 330 SAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKPL 389

Query: 442 PIVFMGGCQNLKKL--------KSIGYKIVVCEDKDGHSLSSQVDNVQSSRVA--LGIFI 501
            +V+  G  +   L          +  KIVVC+      ++++V+     R A  LG+ +
Sbjct: 390 ELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCD----RGVNARVEKGAVVRDAGGLGMIM 449

Query: 502 SN-ISDWDNLIKTP--FPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVA 561
           +N  +  + L+      P+I +    G++++ Y+K  S P A + F  T+L +KP+P VA
Sbjct: 450 ANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVA 509

Query: 562 RYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPH 621
            +SSRGP+   P +LKPD++ PG  ILA W   +    +      T+FN+MSGTSMSCPH
Sbjct: 510 AFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPH 569

Query: 622 AAAVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNP 681
            + +A LLK  H  WSP+ I+SA+MTTA V+DNT   + D  + N  + P A GSG V+P
Sbjct: 570 ISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVDP 629

Query: 682 NKAIDPDLIYDMGIQDYINLLCALNYTKNQIQTITQSASNKCENPSLD---LNYPSFIII 741
            KA+ P L+YD+  ++YI  LC+L+YT + I  I +  S  C     D   LNYPSF ++
Sbjct: 630 QKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVL 689

Query: 742 VNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLS 801
                    KR +   + R +T +G   + Y+  + G   + + VKP KL FK   +K  
Sbjct: 690 FGG------KRVV--RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKR 747

Query: 802 FKLKIAGSVRVKTETDVVYGYLSWAEVGGGHIVQSPI 818
           + +       V       +G ++W+     H V+SP+
Sbjct: 750 YTVTFVSKKGVSMTNKAEFGSITWS--NPQHEVRSPV 747

BLAST of Clc08G17050 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 495.0 bits (1273), Expect = 1.2e-139
Identity = 307/761 (40.34%), Postives = 433/761 (56.90%), Query Frame = 0

Query: 82  YIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKLIHTYNHA 141
           YIVHM  + MP  F    +WY                    SS  S+S  ++L++TY +A
Sbjct: 32  YIVHMAKSQMPSSFDLHSNWY-------------------DSSLRSISDSAELLYTYENA 91

Query: 142 INGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFG-LLPISNCGSD 201
           I+GFS  LT  E ++L   PG +S + +    + TT +  FLGL  +   L P +   SD
Sbjct: 92  IHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSD 151

Query: 202 VIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFFNKGLSA 261
           V++G +DTG+WPES+S++D+G   IPSSWKG CE GT+F+ASLCN+KLIGARFF +G  +
Sbjct: 152 VVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYES 211

Query: 262 KFPNVTIS--MNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRARVAIYKA 321
               +  S    S RD  GHGTHTS+TAAGS VE AS  GY  GTARG+APRARVA+YK 
Sbjct: 212 TMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKV 271

Query: 322 IWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATSA 381
            W  G   SD++AAID+AI+D V+V+S+SLG      Y D VAI  FAA+ERGI V+ SA
Sbjct: 272 CWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSA 331

Query: 382 GNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLFPLNSTMDVSPLPI 441
           GN GP   ++ N APW+  V A T+DRDF     L NG +  G SLF   +  D   LP 
Sbjct: 332 GNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLPF 391

Query: 442 VFMGGCQNLKK----------LKSIGYKIVVCEDKDGHSLSSQVDNVQSSRVALGIFISN 501
           ++ G   N              + +  KIV+C+   G +   Q  +V  +   +G+ ++N
Sbjct: 392 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILAN 451

Query: 502 -ISDWDNLIKTP--FPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTILGIKPAPSVARY 561
             ++ + L+      P+  +    G+II+ Y+    +P A ++   T++G+KP+P VA +
Sbjct: 452 TAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAF 511

Query: 562 SSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNVMSGTSMSCPHAA 621
           SSRGP+   P +LKPD++APG  ILA+W        + S     +FN++SGTSMSCPH +
Sbjct: 512 SSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVS 571

Query: 622 AVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGQVNPNK 681
            +AALLK VH  WSPA IRSA+MTTA         + DI    K +TP   G+G V+P  
Sbjct: 572 GLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT-GKPSTPFDHGAGHVSPTT 631

Query: 682 AIDPDLIYDMGIQDYINLLCALNYTKNQIQTITQSASNKCENPS-----LDLNYPSFIII 741
           A +P LIYD+  +DY+  LCALNYT  QI+++  S  N   +PS      DLNYPSF + 
Sbjct: 632 ATNPGLIYDLTTEDYLGFLCALNYTSPQIRSV--SRRNYTCDPSKSYSVADLNYPSFAVN 691

Query: 742 VNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRG-MKGLKVRVKPKKLEFKRKNQKL 801
           V+   +         ++ RT+T +G    TY  K+     G+K+ V+P  L FK  N+K 
Sbjct: 692 VDGVGAY--------KYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 751

Query: 802 SFKLKIAGSVRVKTETDVVYGYLSWAEVGGGHIVQSPIVVA 821
           S+ +        K      +G + W++  G H+V SP+ ++
Sbjct: 752 SYTVTFTVD-SSKPSGSNSFGSIEWSD--GKHVVGSPVAIS 755

BLAST of Clc08G17050 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 494.6 bits (1272), Expect = 1.6e-139
Identity = 302/775 (38.97%), Postives = 440/775 (56.77%), Query Frame = 0

Query: 75  ATPETDIYIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSLSFPSKL 134
           A+     +I  ++  +MP  F + + WYS        T F +               S++
Sbjct: 21  ASQAAKTFIFRIDGGSMPSIFPTHYHWYS--------TEFAE--------------ESRI 80

Query: 135 IHTYNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSSNFGLLPI 194
           +H Y+   +GFSA +TP E + L+N P  ++   D    + TT S  FLGL +  GL   
Sbjct: 81  VHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSE 140

Query: 195 SNCGSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNKKLIGARFF 254
           S+ GSDVIIG  DTGIWPE  SF+D  +  IP  W+G CE+G  FS   CN+K+IGARFF
Sbjct: 141 SDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFF 200

Query: 255 NKGLSAKF---PNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTARGVAPRA 314
            KG  A      N T+   S RD  GHGTHTS+TAAG +  +AS  GY  G A+GVAP+A
Sbjct: 201 AKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKA 260

Query: 315 RVAIYKAIWEEGNSV-SDVIAAIDQAISDGVDVISLSL-GLDGV--PLYEDPVAIATFAA 374
           R+A YK  W++   + SD++AA D A+ DGVDVIS+S+ G DG+  P Y DP+AI ++ A
Sbjct: 261 RIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGA 320

Query: 375 VERGIFVATSAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSLF-- 434
             +GIFV++SAGN GP   +V N APWV  V A+T+DR+F     L +G  + G SL+  
Sbjct: 321 ASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAG 380

Query: 435 -PLNSTMDVSPLPIVFMGG---------CQNLKKLKSIGYKIVVCEDKDGHSLSSQVDNV 494
            PLN  M     P+V+ G           +N    K +  KIV+C+      ++  +   
Sbjct: 381 VPLNGRM----FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 440

Query: 495 QSSRVALGIFISNISDWDNLIKTP--FPSIFLSPHHGNIIKGYIKKSSDPKAEVNFNKTI 554
           ++  V + I  +  S+ + L+      P+  +  + G+ IK Y     +P A ++F  TI
Sbjct: 441 KAGGVGM-ILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTI 500

Query: 555 LGIKPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVYSSPIYTKFNV 614
           +GIKPAP +A +S RGP+   P +LKPD++APG  ILA+W   V    + S P  T+FN+
Sbjct: 501 VGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNI 560

Query: 615 MSGTSMSCPHAAAVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKDIGNKNKFATP 674
           +SGTSM+CPH +  AALLK  H  WSPA IRSAMMTT ++VDN+  S+ D  +  K ATP
Sbjct: 561 LSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATP 620

Query: 675 LAMGSGQVNPNKAIDPDLIYDMGIQDYINLLCALNYTKNQIQTITQS----ASNKCENPS 734
              GSG +N  +A++P L+YD+   DYI  LC++ Y    IQ IT++     + +  +P 
Sbjct: 621 YDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPG 680

Query: 735 LDLNYPSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRGMKGLKVRVKPKK 794
            +LNYPS   +  ++      + ++    RT T +G+  A Y A++   +G+ V VKP +
Sbjct: 681 -NLNYPSITAVFPTNRRGLVSKTVI----RTATNVGQAEAVYRARIESPRGVTVTVKPPR 740

Query: 795 LEFKRKNQKLSFKLKIAGSVR--VKTETDVVYGYLSWAEVGGGHIVQSPIVVASL 823
           L F    ++ S+ + +  + R  V  ET  V+G ++W + GG H+V+SPIVV  +
Sbjct: 741 LVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVVTQM 761

BLAST of Clc08G17050 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 491.5 bits (1264), Expect = 1.3e-138
Identity = 315/784 (40.18%), Postives = 448/784 (57.14%), Query Frame = 0

Query: 70  SPPQHATPETDI-YIVHMNSAAMPKPFASRHSWYSATISSVLHTSFLDTTSSSSSSSSSL 129
           SP   A+    + YIVH++  A P  F +   WY+++++S+        TSS        
Sbjct: 15  SPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL--------TSS-------- 74

Query: 130 SFPSKLIHTYNHAINGFSASLTPSELEALKNSPGYVSSVLDSSVRVDTTHSSNFLGLSS- 189
             P  +IHTY+   +GFSA LT  +   L + P  +S + +    + TT S  FLGL S 
Sbjct: 75  --PPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRST 134

Query: 190 -NFGLLPISNCGSDVIIGFVDTGIWPESESFNDDGMSEIPSSWKGECENGTHFSASLCNK 249
              GLL  S+ GSD++IG +DTG+WPE  SF+D G+  +P  WKG+C     F  S CN+
Sbjct: 135 DKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNR 194

Query: 250 KLIGARFFNKGLSAK--FPNVTISMNSTRDTIGHGTHTSTTAAGSYVEEASFFGYGQGTA 309
           KL+GARFF  G  A     N T    S RD+ GHGTHT++ +AG YV  AS  GY  G A
Sbjct: 195 KLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 254

Query: 310 RGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIAT 369
            G+AP+AR+A YK  W  G   SD++AA D A++DGVDVISLS+G   VP Y D +AI  
Sbjct: 255 AGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGA 314

Query: 370 FAAVERGIFVATSAGNNGPKLGTVHNGAPWVLNVAAATMDRDFGGTITLSNGVSIFGSSL 429
           F A++RGIFV+ SAGN GP   TV N APW+  V A T+DRDF   + L NG  I G S+
Sbjct: 315 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 374

Query: 430 FPLNSTMDVSPLPIVF----MGGCQNLKKL--------KSIGYKIVVCEDKDGHSLSSQV 489
           +           P+V+    +GG      L          +  KIV+C D+  +S +++ 
Sbjct: 375 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLC-DRGINSRATKG 434

Query: 490 DNVQSSRVALGIFISN-ISDWDNLIKT--PFPSIFLSPHHGNIIKGYIKKSS------DP 549
           + V+ +   LG+ I+N + D + L+      P+  +    G+ I+ YI +SS       P
Sbjct: 435 EIVRKNG-GLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHP 494

Query: 550 KAEVNFNKTILGIKPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMNVY 609
            A + F  T LGI+PAP VA +S+RGP+   P +LKPD++APG  ILA+WP  +    V 
Sbjct: 495 TATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVT 554

Query: 610 SSPIYTKFNVMSGTSMSCPHAAAVAALLKGVHRRWSPATIRSAMMTTADVVDNTQTSIKD 669
           S    T+FN++SGTSM+CPH + +AALLK  H  WSPA IRSA++TTA  VDN+   + D
Sbjct: 555 SDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD 614

Query: 670 IGNKNKFATPLAMGSGQVNPNKAIDPDLIYDMGIQDYINLLCALNYTKNQIQTITQSASN 729
               N  ++ +  GSG V+P KA+DP L+YD+   DYIN LC  NYT+  I TIT+  ++
Sbjct: 615 ESTGNT-SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD 674

Query: 730 KCENPSL-----DLNYPSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKRATYEAKLRGM 789
            C+         +LNYPSF ++      +  + ++   F RT+T +G+  + YE K+R  
Sbjct: 675 -CDGARRAGHVGNLNYPSFSVVF----QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPP 734

Query: 790 KGLKVRVKPKKLEFKRKNQKLSFKLKIAGSVRVKTE---TDVVYGYLSWAEVGGGHIVQS 820
           +G  V V+P+KL F+R  QKLSF +++  +  VK     T+V  G++ W++  G   V S
Sbjct: 735 RGTTVTVEPEKLSFRRVGQKLSFVVRVK-TTEVKLSPGATNVETGHIVWSD--GKRNVTS 769