Clc08G12400 (gene) Watermelon (cordophanus) v2

Overview
NameClc08G12400
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionPoly(A) polymerase
LocationClcChr08: 23677952 .. 23681815 (+)
RNA-Seq ExpressionClc08G12400
SyntenyClc08G12400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATACAACTAACTAATTAAGATTAAAATTGGACAATTCAGAGTGGAACTAACTAAGATTAAATGACTTTCAATGATATGTTAAACGTAATTATTATTAAAAAGAAATAAAAAAAATTACAAAGACAAGCACTCCAAAGTGCAAAAACTAAACCCTTTTCCCCGCTTCGACGCCCTCTCTCCTTCATCTTCCTCTCTCTCTCTCTCTCTGTGCAGCCCTGAGTTTGACCGGTTTCCGAACCGTCAACCCATCGCCCGTACGGATTAAGCCTTCGATCTGTTCTCTCTTCAAACTTCTCCGACCTTCTCTTTCACCGCAGCCCGTTTCCTTCTGCCTCGTTTGCTTCTCTATGCTTTCTAATTCACCTTCTCGCATCAAATCTTGATCCCAATTGTTCCATTTCATCTTCTAATTTTGGGGCACCCACATCGCAAATGCTTTGCTGAGTTAGCTTCAGTTTTCTCTCATTTGCTCGGATCTTTTTCGTCAATTCTCGGTCAGATTCAGGGTTGCTGCGTGATTCGCTGTCTTTGCGTTAGCTGTTTCTTTTTCGTTCTTTAGGAAATGGTGGGTTCTGACAGTTCACCTGGTTTTCCGCCGGCGCCGAATTATGGTGTTACGAAACCGATTTCGCTTGCTGGGCCGACGGATGCTGATATTCAGCGCAATATCGAACTGGAGAAGGTGGTATTTACTTTTGTTTTGTTTGGTTAAATCTCAATTGTTTTGGTGGGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGTGCTGTGTGAAATTCCAGTAAATGAATTGAATTTCAATTTTGATTATGTTGTACGTATTGGTAGTTCTTGATTGACTCGGGGCTTTACGAAAGTAAAGAAGAAGCTGCGAAAAGAGAAGAGGTTCTAGGCCGCATTGACCAGGTGCAGACCTTGTTCACTTATATAGTCTTTTTTATTTTTGTTCTTTATGGAAATATTGGACTTTGCTCTCAATCCCACCTTCAACAAAACAGTGTTCTAATCAGTGACGCAAATGAGATTTTAGTTTATTATTATTGTTATTATTTTTTTAATGTTCAACGTTTTACTCCCGTTTATGTCATGAACTTCTTATCCTTAGTCATTTATCTTTTTATTCTAGATCGTTAAAAATTGGGTCAAGCAATTAACCCTCCTTAGAGGATACACAGAGCAGATGGTGGAAGATGCAAATGCTGTAATTTTTACTTTTGGGTCTTACCGTCTTGGGGTAAGCATAATATTTGATTAAGTTTTTGGGGAAAAAAACGTACTTTTGAGTTTGACATTATGTAACTCTTTGTACTTTCTCTTGTTGGGGCGTACAAACTGTTGGAATGTATGCTTGCTTAGAGCAACGATCTTTTAGTTCTTGGAAAGTTATGGTCTTCTTGTATTTTTGCCTTATTGGCCTGTGCATTTTATTCAAATATATGACTTATAAGTAGCCAAGGATGTGGCATAAAATTAACCATATGACAGGTACATGGACCAGGAAGTGACATAGACACTTTGTGTGTTGGACCATCTTACGTGAATAGAGAAGTGAGTAGTATAAAATGCTAATATTTTCAATTATATATAAATTGTTGATACTATCAACAATTTTTTTCACCTCCTTATGGGATTAAATTGAAACTTATTATTTATCTCAATTTGTTTGTTTCGAATGGAGGACTTCTTCATCATCCTGCATAATATTTTGGCCGAAATGGAAGAGGTTGCCGATCTTCAACCAGTTCCAGATGCTCATGTTCCAGTGATGAGATTCAAATTTCTGGGAATATCAATTGATCTTCTGTATGCAAGCATTTCTGCACTGGTTGTTCCAGAAGTAAGTTTTAGCAGTTTGTTTGAAGTTTTATCATATTTATTTTATGGTTTCAGAATTTATTGTTGTTATTTTCCCCCCCACAAAACTGTAACTGTATTTCTTTTTGTTACATCATTGTCGTTGATCATATGAGATTTCAAACTATTGTATATTAAATGCTCTCTCTCTATTTCTACATTTCTCTTAATGACGAATATTTCCATAATTCATGCAGAATTACCTGGCTTATAAAACTCCAATTGCCAAACTTTGTTATTTGAATAATCATGATGGCACTTGTCATTATAAATATGGAAATTCTTTGCAGTTTTATTTATAGTTTGAATCATTTCTACCAGCTTTTGTAAGTTCATTGTACCCTCCAGTGGTTGAGTTATATATTTGTTTCCCCCCCTTGCCCAACAAATGTGGTCTTGATCTACTCTTCAGGGATTTCTTTCTTGTATGTTATTTTATTTTATTATTATATGGATCGTGGCTGGGTAGAGAAAATTCACTCCCGAGAAGACTATTATTTACCAAAGGAATTTCTTTCTTCTCTCTTAACCTCATTCCCAACATTTTATTATCTGTCAAGTTGGTATTACTACCTTGAGTTTGTGTAGGTGAATTAATTGTTTGCTATGTGTGTATATGCGTTTGTTAACACTGAACAGGTGATACTATTTTGCATACCCTTTCTGTTGTAGAGTTAACTATGATTGGGTGGTATCACTTACTAATTGTTTTCCTACTACAATTCTCCCTTTTCCCCCCTGTCTTTTGGGCTGTTGCTATTTCCCATAAAAATTCTCTGCAAATTACTTAACCATCAGGACCTGGACATCTCTCATGGATCTGTGTTGTATAATGTTGATGAACAAACTGTTCGAAGTCTTAATGGATGCAGAGTTGCAGATCAGATTTTAAGACTTGTTCCAAACGTTGAGGTGAAAGTGGAAAACACATTTTGAGCCTGAGTTGTTTTACCATAAACCTATTCCTGATTTTTTCTGTAACATATAATTGTAGCACTTTCGCACGACAACTCAGATGGTTTAAAGTTTTGGGCCAAACGACGGGGGTGTATATTCAAATGTAAGCTGTATTATCATGGAGTTTTGCCTCTTGTTTCCACGTATGTTGTTCATATTTCACTTTCAATTTTTGTTTTTGTTTCTTGTATAGGTCACTGGGTTCCTTGGAGGTGTAAATTGGGCACTTTTGGTTGCTCAGGTATGCCAGCTTTACCCCAATGCAATTCCAAGCATGCTAGTTTCTCGTTTCTTCAGAGTGTACACCCAGTGGCGTTGGCCCAAATCCTGTAATGCTATGTTCAATAGAAGAGAATGAAATTGGATTTCCTGTTTGGGATCCTCGTAAAAATCCTCGTGATCGATTTCATCTATGCCAATAATAACTCCATGCTTACCCCTTGCATgaactctagctaTTTGTCTCAACTAGTACTCTTCGTGTTATGATGGAACAGTTCCGCTATGGTATAACATCTGTGAGGTGAGGTATTTTTATTTGGATTCAAAATATTCTAAATATTAGCCTGCTCTTAATAGGTATTTTCTCTTACGTTCTGAGTGCCTTACAGGAGATTGACATGAAGCAAAGGCCCAGTGGAGTGTTTTGTTTGAGCCATATTTATTTTTCGAGACATACAAAAACTATCTACAGGTGGATATAATCGCAGCAGATGCTGACGATTTGCTAGCTTGGAAAGGATGGGTTGAATTCTCGCTTAGAACAGCTTACTGAAGGTAAATCTAATTAGCCCTTTCCTGATTATCTAATCTGAAGTTGGAGTGTCTTTCAACAGCCTGATATGTCTACTTTTCCTTCCTGCAGATAGAGAGGGACACAGGGGAATGCTGCAGTGCCATCCCTATCCTATTGAGTATTCTGACACGGTCCAAGCCATGTTCTCACTGTGCTTTTTTCATGGGCTTGCAGAGAAAAGAGGGATTAAGAGGCCAAGGAGGCAGCAGTTTGACATACGGGGAACTGTTGATGAGTTCCGGCAGAGAGATTAA

mRNA sequence

AATACAACTAACTAATTAAGATTAAAATTGGACAATTCAGAGTGGAACTAACTAAGATTAAATGACTTTCAATGATATGTTAAACGTAATTATTATTAAAAAGAAATAAAAAAAATTACAAAGACAAGCACTCCAAAGTGCAAAAACTAAACCCTTTTCCCCGCTTCGACGCCCTCTCTCCTTCATCTTCCTCTCTCTCTCTCTCTCTGTGCAGCCCTGAGTTTGACCGGTTTCCGAACCGTCAACCCATCGCCCGTACGGATTAAGCCTTCGATCTGTTCTCTCTTCAAACTTCTCCGACCTTCTCTTTCACCGCAGCCCGTTTCCTTCTGCCTCGTTTGCTTCTCTATGCTTTCTAATTCACCTTCTCGCATCAAATCTTGATCCCAATTGTTCCATTTCATCTTCTAATTTTGGGGCACCCACATCGCAAATGCTTTGCTGAGTTAGCTTCAGTTTTCTCTCATTTGCTCGGATCTTTTTCGTCAATTCTCGGTCAGATTCAGGGTTGCTGCGTGATTCGCTGTCTTTGCGTTAGCTGTTTCTTTTTCGTTCTTTAGGAAATGGTGGGTTCTGACAGTTCACCTGGTTTTCCGCCGGCGCCGAATTATGGTGTTACGAAACCGATTTCGCTTGCTGGGCCGACGGATGCTGATATTCAGCGCAATATCGAACTGGAGAAGTTCTTGATTGACTCGGGGCTTTACGAAAGTAAAGAAGAAGCTGCGAAAAGAGAAGAGGTTCTAGGCCGCATTGACCAGATCGTTAAAAATTGGGTCAAGCAATTAACCCTCCTTAGAGGATACACAGAGCAGATGGTGGAAGATGCAAATGCTGTAATTTTTACTTTTGGGTCTTACCGTCTTGGGGTACATGGACCAGGAAGTGACATAGACACTTTGTGTGTTGGACCATCTTACGTGAATAGAGAAGACTTCTTCATCATCCTGCATAATATTTTGGCCGAAATGGAAGAGGTTGCCGATCTTCAACCAGTTCCAGATGCTCATGTTCCAGTGATGAGATTCAAATTTCTGGGAATATCAATTGATCTTCTGTATGCAAGCATTTCTGCACTGGTTGTTCCAGAAGACCTGGACATCTCTCATGGATCTGTGTTGTATAATGTTGATGAACAAACTGTTCGAAGTCTTAATGGATGCAGAGTTGCAGATCAGATTTTAAGACTTGTTCCAAACGTTGAGGTCACTGGGTTCCTTGGAGGTGTAAATTGGGCACTTTTGGTTGCTCAGCTTGGAAAGGATGGGTTGAATTCTCGCTTAGAACAGCTTACTGAAGATAGAGAGGGACACAGGGGAATGCTGCAGTGCCATCCCTATCCTATTGAGTATTCTGACACGGTCCAAGCCATGTTCTCACTGTGCTTTTTTCATGGGCTTGCAGAGAAAAGAGGGATTAAGAGGCCAAGGAGGCAGCAGTTTGACATACGGGGAACTGTTGATGAGTTCCGGCAGAGAGATTAA

Coding sequence (CDS)

ATGGTGGGTTCTGACAGTTCACCTGGTTTTCCGCCGGCGCCGAATTATGGTGTTACGAAACCGATTTCGCTTGCTGGGCCGACGGATGCTGATATTCAGCGCAATATCGAACTGGAGAAGTTCTTGATTGACTCGGGGCTTTACGAAAGTAAAGAAGAAGCTGCGAAAAGAGAAGAGGTTCTAGGCCGCATTGACCAGATCGTTAAAAATTGGGTCAAGCAATTAACCCTCCTTAGAGGATACACAGAGCAGATGGTGGAAGATGCAAATGCTGTAATTTTTACTTTTGGGTCTTACCGTCTTGGGGTACATGGACCAGGAAGTGACATAGACACTTTGTGTGTTGGACCATCTTACGTGAATAGAGAAGACTTCTTCATCATCCTGCATAATATTTTGGCCGAAATGGAAGAGGTTGCCGATCTTCAACCAGTTCCAGATGCTCATGTTCCAGTGATGAGATTCAAATTTCTGGGAATATCAATTGATCTTCTGTATGCAAGCATTTCTGCACTGGTTGTTCCAGAAGACCTGGACATCTCTCATGGATCTGTGTTGTATAATGTTGATGAACAAACTGTTCGAAGTCTTAATGGATGCAGAGTTGCAGATCAGATTTTAAGACTTGTTCCAAACGTTGAGGTCACTGGGTTCCTTGGAGGTGTAAATTGGGCACTTTTGGTTGCTCAGCTTGGAAAGGATGGGTTGAATTCTCGCTTAGAACAGCTTACTGAAGATAGAGAGGGACACAGGGGAATGCTGCAGTGCCATCCCTATCCTATTGAGTATTCTGACACGGTCCAAGCCATGTTCTCACTGTGCTTTTTTCATGGGCTTGCAGAGAAAAGAGGGATTAAGAGGCCAAGGAGGCAGCAGTTTGACATACGGGGAACTGTTGATGAGTTCCGGCAGAGAGATTAA

Protein sequence

MVGSDSSPGFPPAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAKREEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVGPSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPEDLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVEVTGFLGGVNWALLVAQLGKDGLNSRLEQLTEDREGHRGMLQCHPYPIEYSDTVQAMFSLCFFHGLAEKRGIKRPRRQQFDIRGTVDEFRQRD
Homology
BLAST of Clc08G12400 vs. NCBI nr
Match: XP_022132220.1 (nuclear poly(A) polymerase 2-like isoform X2 [Momordica charantia])

HSP 1 Score: 469.5 bits (1207), Expect = 2.1e-128
Identity = 259/369 (70.19%), Postives = 265/369 (71.82%), Query Frame = 0

Query: 1   MVGSDSSPGF----PPAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           M GSDSS G      PA   GVTKPISLAGPTDADIQRN ELEKFLIDSGLYESKEEA  
Sbjct: 1   MAGSDSSTGILLAPQPARKNGVTKPISLAGPTDADIQRNTELEKFLIDSGLYESKEEATN 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILHNILAEMEEV DLQPVPDAHVPVMRFKF GISIDLLYASIS LVVPE
Sbjct: 121 PSYVNREDFFIILHNILAEMEEVTDLQPVPDAHVPVMRFKFQGISIDLLYASISLLVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------V 240
           DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE                     V
Sbjct: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVEHFRTTLRCLKFWAKRRGVYSNV 240

Query: 241 TGFLGGVNWALLVAQ---------------------------------------LGKDGL 300
           TGFLGGVNWALLVAQ                                         K  +
Sbjct: 241 TGFLGGVNWALLVAQEIELSKAQWSALFEPYLFFETYKNYLQVDMIAADADDLLAWKGWV 300

Query: 301 NSRLEQLT-EDREGHRGMLQCHPYPIEYSDTVQAMFSLCFFHGLAEKRGIKRPRRQQFDI 305
            SR  QLT +     RGMLQCHPYPIEYSDT +  +   FF GL  K G++    QQFDI
Sbjct: 301 ESRFRQLTLKIERDTRGMLQCHPYPIEYSDTSKPCYHCAFFMGLQRKEGLRGQGGQQFDI 360

BLAST of Clc08G12400 vs. NCBI nr
Match: XP_038884467.1 (nuclear poly(A) polymerase 4-like isoform X3 [Benincasa hispida])

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-123
Identity = 266/463 (57.45%), Postives = 275/463 (59.40%), Query Frame = 0

Query: 1   MVGSDSSPGFP----PAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVGSD+SPGFP    PA NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK
Sbjct: 1   MVGSDTSPGFPSAPQPATNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASIS LVVPE
Sbjct: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISVLVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------V 240
           DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPN+E                     +
Sbjct: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNIEHFRTTLRCLKFWAKRRGVYSNI 240

Query: 241 TGFLGGVNWALLVAQL-------------------------------------------- 300
           TGFLGGVNWALLVAQ+                                            
Sbjct: 241 TGFLGGVNWALLVAQVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEENELGFPVW 300

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 301 DPRRNPRDRFHLMPIITPAYPCMNSSYNVSTSTLRVMMEQFRYGNSICEEIDLSKAQWSA 360

BLAST of Clc08G12400 vs. NCBI nr
Match: XP_008444997.1 (PREDICTED: nuclear poly(A) polymerase 4 isoform X3 [Cucumis melo])

HSP 1 Score: 442.6 bits (1137), Expect = 2.8e-120
Identity = 262/463 (56.59%), Postives = 270/463 (58.32%), Query Frame = 0

Query: 1   MVGSDSSPGFP----PAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVGSD S G P    PA NYGVTKPISLAGPTDADI RNIELEKFL+DSGLYESKEEAAK
Sbjct: 1   MVGSDCSTGLPSVSHPATNYGVTKPISLAGPTDADIHRNIELEKFLVDSGLYESKEEAAK 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILH+ILAEMEEV DLQPVPDAHVPVMRFKFLGISIDLLYASIS LVVPE
Sbjct: 121 PSYVNREDFFIILHDILAEMEEVTDLQPVPDAHVPVMRFKFLGISIDLLYASISLLVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNV---------------------EV 240
           DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNV                      V
Sbjct: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVGHFRTTLRCLKFWAKRRGVYSNV 240

Query: 241 TGFLGGVNWALLVAQL-------------------------------------------- 300
           TGFLGGVNWALLVAQ+                                            
Sbjct: 241 TGFLGGVNWALLVAQVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEENELGFPVW 300

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 301 DPRRNPRDRFHLMPIITPAYPCMNSSYNVSTSTLRVMMEQFCYGNNICEEIDLSKAQWSA 360

BLAST of Clc08G12400 vs. NCBI nr
Match: XP_038884466.1 (nuclear poly(A) polymerase 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 442.2 bits (1136), Expect = 3.7e-120
Identity = 266/493 (53.96%), Postives = 275/493 (55.78%), Query Frame = 0

Query: 1   MVGSDSSPGFP----PAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVGSD+SPGFP    PA NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK
Sbjct: 1   MVGSDTSPGFPSAPQPATNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASIS LVVPE
Sbjct: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISVLVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------V 240
           DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPN+E                     +
Sbjct: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNIEHFRTTLRCLKFWAKRRGVYSNI 240

Query: 241 TGFLGGVNWALLVAQL-------------------------------------------- 300
           TGFLGGVNWALLVAQ+                                            
Sbjct: 241 TGFLGGVNWALLVAQVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEENELGFPVW 300

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 301 DPRRNPRDRFHLMPIITPAYPCMNSSYNVSTSTLRVMMEQFRYGNSICEVRLFYIWIQNS 360

BLAST of Clc08G12400 vs. NCBI nr
Match: XP_038884464.1 (nuclear poly(A) polymerase 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 442.2 bits (1136), Expect = 3.7e-120
Identity = 266/493 (53.96%), Postives = 275/493 (55.78%), Query Frame = 0

Query: 1   MVGSDSSPGFP----PAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVGSD+SPGFP    PA NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK
Sbjct: 1   MVGSDTSPGFPSAPQPATNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASIS LVVPE
Sbjct: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISVLVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------V 240
           DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPN+E                     +
Sbjct: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNIEHFRTTLRCLKFWAKRRGVYSNI 240

Query: 241 TGFLGGVNWALLVAQL-------------------------------------------- 300
           TGFLGGVNWALLVAQ+                                            
Sbjct: 241 TGFLGGVNWALLVAQVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEENELGFPVW 300

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 301 DPRRNPRDRFHLMPIITPAYPCMNSSYNVSTSTLRVMMEQFRYGNSICEVRLFYIWIQNS 360

BLAST of Clc08G12400 vs. ExPASy Swiss-Prot
Match: Q8VYW1 (Nuclear poly(A) polymerase 4 OS=Arabidopsis thaliana OX=3702 GN=PAPS4 PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.8e-99
Identity = 223/464 (48.06%), Postives = 248/464 (53.45%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAPN----YGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVG+ +  G  P  N    YG+TKP+SLAGP+ ADI+RN+ELEK+L+D GLYESK++  +
Sbjct: 2   MVGTQNLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMR 61

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVK+WVKQLT  RGYT+QMVEDANAVIFTFGSYRLGVHGPG+DIDTLCVG
Sbjct: 62  REEVLGRIDQIVKHWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVG 121

Query: 121 PSYVNR-EDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVP 180
           PSYVNR EDFFIILH+ILAEMEEV +L PVPDAHVPVM+FKF GI IDLLYASIS LVVP
Sbjct: 122 PSYVNREEDFFIILHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVP 181

Query: 181 EDLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE--------------------- 240
           +DLDIS  SVL  VDE TVRSLNGCRVADQIL+LVPN E                     
Sbjct: 182 QDLDISSSSVLCEVDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSN 241

Query: 241 VTGFLGGVNWALLVA---------------------------------------QLG--- 300
           VTGFLGGVNWALLVA                                       +LG   
Sbjct: 242 VTGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPV 301

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 302 WDRRKNHRDRYHLMPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWS 361

BLAST of Clc08G12400 vs. ExPASy Swiss-Prot
Match: O82312 (Nuclear poly(A) polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=PAPS2 PE=1 SV=2)

HSP 1 Score: 345.9 bits (886), Expect = 4.7e-94
Identity = 213/455 (46.81%), Postives = 240/455 (52.75%), Query Frame = 0

Query: 6   SSPGFPPAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAKREEVLGRID 65
           S P      +YG+T+P+S+AGP+ AD++RN+ELEKFL+D GLYESKEE  +REEV+ RID
Sbjct: 13  SQPVKASLKSYGITEPLSIAGPSAADVKRNLELEKFLVDEGLYESKEETMRREEVVVRID 72

Query: 66  QIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVGPSYVNR-ED 125
           QIVK+WVKQLT  RGYT+QMVEDANAVIFTFGSYRLGVHGP +DIDTLCVGPSYVNR ED
Sbjct: 73  QIVKHWVKQLTRQRGYTDQMVEDANAVIFTFGSYRLGVHGPMADIDTLCVGPSYVNREED 132

Query: 126 FFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPEDLDISHGS 185
           FFI   +ILAEMEEV +LQPV DAHVPVM+FKF GISIDLLYASIS LV+P+DLDIS+ S
Sbjct: 133 FFIFFRDILAEMEEVTELQPVTDAHVPVMKFKFQGISIDLLYASISLLVIPQDLDISNSS 192

Query: 186 VLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------VTGFLGGVN 245
           VL +VDEQTVRSLNGCRVADQIL+LVPN E                     VTGFLGGVN
Sbjct: 193 VLCDVDEQTVRSLNGCRVADQILKLVPNSEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVN 252

Query: 246 WALLVAQL---------------------------------------------------- 305
           WALLVA+L                                                    
Sbjct: 253 WALLVARLCQFYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDDLSFPVWDPRKNHRD 312

BLAST of Clc08G12400 vs. ExPASy Swiss-Prot
Match: Q9LMT2 (Nuclear poly(A) polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=PAPS1 PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.4e-76
Identity = 142/238 (59.66%), Postives = 176/238 (73.95%), Query Frame = 0

Query: 16  YGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAKREEVLGRIDQIVKNWVKQL 75
           +GV++PIS+ GPT+ D+ +  ELEK L D GLYESKEEA +REEVLG +DQIVK W+K +
Sbjct: 11  FGVSEPISMGGPTEFDVIKTRELEKHLQDVGLYESKEEAVRREEVLGILDQIVKTWIKTI 70

Query: 76  TLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVGPSYVNRE-DFFIILHNILA 135
           +  +G  +Q++ +ANA IFTFGSYRLGVHGPG+DIDTLCVGP +  RE DFF  L  +L+
Sbjct: 71  SRAKGLNDQLLHEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREGDFFGELQRMLS 130

Query: 136 EMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPEDLDISHGSVLYNVDEQTV 195
           EM EV +L PVPDAHVP+M FK  G+SIDLLYA +   V+PEDLD+S  S+L N DEQTV
Sbjct: 131 EMPEVTELHPVPDAHVPLMGFKLNGVSIDLLYAQLPLWVIPEDLDLSQDSILQNADEQTV 190

Query: 196 RSLNGCRVADQILRLVPNVE---------------------VTGFLGGVNWALLVAQL 232
           RSLNGCRV DQILRLVPN++                     V+GFLGG+NWALLVA++
Sbjct: 191 RSLNGCRVTDQILRLVPNIQNFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARI 248

BLAST of Clc08G12400 vs. ExPASy Swiss-Prot
Match: Q54J73 (Poly(A) polymerase OS=Dictyostelium discoideum OX=44689 GN=papA PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 5.8e-60
Identity = 119/237 (50.21%), Postives = 156/237 (65.82%), Query Frame = 0

Query: 17  GVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAKREEVLGRIDQIVKNWVKQLT 76
           GVT+PIS A P+  D + + ELE  LI   L+ES EE+ KREE+LG+++QIV+ W KQ++
Sbjct: 54  GVTEPISTAPPSSIDFKLSTELENTLISFNLFESPEESRKREEILGKLNQIVREWAKQVS 113

Query: 77  LLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVGPSYVNREDFFIILHNILAEM 136
           L +GY EQ   +  A IFTFGSYRLGVHGPGSDIDTLCVGP ++ R DFF  L +IL   
Sbjct: 114 LKKGYPEQTASEVVAKIFTFGSYRLGVHGPGSDIDTLCVGPKHIMRSDFFDDLSDILKVH 173

Query: 137 EEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPEDL-DISHGSVLYNVDEQTVR 196
            E+ +   V DA VPV+   F GI IDL+YA ++   +PE+L D+   S L N+DE+++ 
Sbjct: 174 PEITEFTTVKDAFVPVITMVFSGIPIDLIYAKLALTAIPEELNDLIDESFLKNIDEKSIL 233

Query: 197 SLNGCRVADQILRLVPNV---------------------EVTGFLGGVNWALLVAQL 232
           SLNGCRV DQIL+LVPN+                      + GFLGGV+WALL A++
Sbjct: 234 SLNGCRVTDQILKLVPNIPNFRMALRCIKLWAIRRGIYSNILGFLGGVSWALLTARI 290

BLAST of Clc08G12400 vs. ExPASy Swiss-Prot
Match: P25500 (Poly(A) polymerase alpha OS=Bos taurus OX=9913 GN=PAPOLA PE=1 SV=3)

HSP 1 Score: 209.1 bits (531), Expect = 6.8e-53
Identity = 103/241 (42.74%), Postives = 153/241 (63.49%), Query Frame = 0

Query: 11  PPAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAKREEVLGRIDQIVKN 70
           PP  +YG+T PISLA P + D     +L + L   G++E +EE  +R  +LG+++ +VK 
Sbjct: 15  PPQKHYGITSPISLAAPKETDCLLTQKLVETLKPFGVFEEEEELQRRILILGKLNNLVKE 74

Query: 71  WVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVGPSYVNREDFFIILH 130
           W+++++  +   + ++E+    IFTFGSYRLGVH  G+DID LCV P +V+R DFF   +
Sbjct: 75  WIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFY 134

Query: 131 NILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPEDLDISHGSVLYNVD 190
           + L   EEV DL+ V +A VPV++  F GI ID+L+A ++   +PEDLD+   S+L N+D
Sbjct: 135 DKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLD 194

Query: 191 EQTVRSLNGCRVADQILRLVPNVE---------------------VTGFLGGVNWALLVA 231
            + +RSLNGCRV D+IL LVPN++                     + GFLGGV+WA+LVA
Sbjct: 195 IRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVA 254

BLAST of Clc08G12400 vs. ExPASy TrEMBL
Match: A0A6J1BRU6 (Polynucleotide adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111005135 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.0e-128
Identity = 259/369 (70.19%), Postives = 265/369 (71.82%), Query Frame = 0

Query: 1   MVGSDSSPGF----PPAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           M GSDSS G      PA   GVTKPISLAGPTDADIQRN ELEKFLIDSGLYESKEEA  
Sbjct: 1   MAGSDSSTGILLAPQPARKNGVTKPISLAGPTDADIQRNTELEKFLIDSGLYESKEEATN 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILHNILAEMEEV DLQPVPDAHVPVMRFKF GISIDLLYASIS LVVPE
Sbjct: 121 PSYVNREDFFIILHNILAEMEEVTDLQPVPDAHVPVMRFKFQGISIDLLYASISLLVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------V 240
           DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE                     V
Sbjct: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVEHFRTTLRCLKFWAKRRGVYSNV 240

Query: 241 TGFLGGVNWALLVAQ---------------------------------------LGKDGL 300
           TGFLGGVNWALLVAQ                                         K  +
Sbjct: 241 TGFLGGVNWALLVAQEIELSKAQWSALFEPYLFFETYKNYLQVDMIAADADDLLAWKGWV 300

Query: 301 NSRLEQLT-EDREGHRGMLQCHPYPIEYSDTVQAMFSLCFFHGLAEKRGIKRPRRQQFDI 305
            SR  QLT +     RGMLQCHPYPIEYSDT +  +   FF GL  K G++    QQFDI
Sbjct: 301 ESRFRQLTLKIERDTRGMLQCHPYPIEYSDTSKPCYHCAFFMGLQRKEGLRGQGGQQFDI 360

BLAST of Clc08G12400 vs. ExPASy TrEMBL
Match: A0A1S3BBN8 (Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103488167 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.4e-120
Identity = 262/463 (56.59%), Postives = 270/463 (58.32%), Query Frame = 0

Query: 1   MVGSDSSPGFP----PAPNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVGSD S G P    PA NYGVTKPISLAGPTDADI RNIELEKFL+DSGLYESKEEAAK
Sbjct: 1   MVGSDCSTGLPSVSHPATNYGVTKPISLAGPTDADIHRNIELEKFLVDSGLYESKEEAAK 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILH+ILAEMEEV DLQPVPDAHVPVMRFKFLGISIDLLYASIS LVVPE
Sbjct: 121 PSYVNREDFFIILHDILAEMEEVTDLQPVPDAHVPVMRFKFLGISIDLLYASISLLVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNV---------------------EV 240
           DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNV                      V
Sbjct: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVGHFRTTLRCLKFWAKRRGVYSNV 240

Query: 241 TGFLGGVNWALLVAQL-------------------------------------------- 300
           TGFLGGVNWALLVAQ+                                            
Sbjct: 241 TGFLGGVNWALLVAQVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEENELGFPVW 300

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 301 DPRRNPRDRFHLMPIITPAYPCMNSSYNVSTSTLRVMMEQFCYGNNICEEIDLSKAQWSA 360

BLAST of Clc08G12400 vs. ExPASy TrEMBL
Match: A0A6J1K7X6 (Polynucleotide adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491975 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 5.2e-120
Identity = 262/463 (56.59%), Postives = 271/463 (58.53%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAP----NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVGSDSS  F PAP    NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK
Sbjct: 1   MVGSDSSTAFHPAPQPASNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILHNILAEMEEV DLQPVPDAHVPVMRFKF+GISIDLLYASIS  VVPE
Sbjct: 121 PSYVNREDFFIILHNILAEMEEVTDLQPVPDAHVPVMRFKFMGISIDLLYASISVRVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------V 240
           +L+ISHGSVL NVDEQTVRSLNGCRVADQILRLVPNVE                     V
Sbjct: 181 ELNISHGSVLCNVDEQTVRSLNGCRVADQILRLVPNVEHFRTTLRCLKFWAKRRGVYSNV 240

Query: 241 TGFLGGVNWALLVAQL-------------------------------------------- 300
           TGFLGGVNWALLVAQ+                                            
Sbjct: 241 TGFLGGVNWALLVAQVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEENELGFPVW 300

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 301 DPRKNPRDRFHLMPIITPAYPCMNSSYNVSTSTLRVMMEQFRCGNNICEDIDMSKAQWSA 360

BLAST of Clc08G12400 vs. ExPASy TrEMBL
Match: A0A6J1HCH8 (Polynucleotide adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462377 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 5.2e-120
Identity = 262/463 (56.59%), Postives = 271/463 (58.53%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAP----NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVGSDSS  F PAP    NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK
Sbjct: 1   MVGSDSSTAFHPAPQPASNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILHNILAEMEEV DLQPVPDAHVPVMRFKF+GISIDLLYASIS  VVPE
Sbjct: 121 PSYVNREDFFIILHNILAEMEEVTDLQPVPDAHVPVMRFKFMGISIDLLYASISVRVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------V 240
           +L+ISHGSVL NVDEQTVRSLNGCRVADQILRLVPNVE                     V
Sbjct: 181 ELNISHGSVLCNVDEQTVRSLNGCRVADQILRLVPNVEHFRTTLRCLKFWAKRRGVYSNV 240

Query: 241 TGFLGGVNWALLVAQL-------------------------------------------- 300
           TGFLGGVNWALLVAQ+                                            
Sbjct: 241 TGFLGGVNWALLVAQVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEENELGFPVW 300

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 301 DPRKNPRDRFHLMPIITPAYPCMNSSYNVSTSTLRVMMEQFRCGNNICEEIDMSKAQWSA 360

BLAST of Clc08G12400 vs. ExPASy TrEMBL
Match: A0A6J1KC70 (Polynucleotide adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491975 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 5.2e-120
Identity = 262/463 (56.59%), Postives = 271/463 (58.53%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAP----NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVGSDSS  F PAP    NYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK
Sbjct: 1   MVGSDSSTAFHPAPQPASNYGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG
Sbjct: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120

Query: 121 PSYVNREDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVPE 180
           PSYVNREDFFIILHNILAEMEEV DLQPVPDAHVPVMRFKF+GISIDLLYASIS  VVPE
Sbjct: 121 PSYVNREDFFIILHNILAEMEEVTDLQPVPDAHVPVMRFKFMGISIDLLYASISVRVVPE 180

Query: 181 DLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE---------------------V 240
           +L+ISHGSVL NVDEQTVRSLNGCRVADQILRLVPNVE                     V
Sbjct: 181 ELNISHGSVLCNVDEQTVRSLNGCRVADQILRLVPNVEHFRTTLRCLKFWAKRRGVYSNV 240

Query: 241 TGFLGGVNWALLVAQL-------------------------------------------- 300
           TGFLGGVNWALLVAQ+                                            
Sbjct: 241 TGFLGGVNWALLVAQVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEENELGFPVW 300

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 301 DPRKNPRDRFHLMPIITPAYPCMNSSYNVSTSTLRVMMEQFRCGNNICEDIDMSKAQWSA 360

BLAST of Clc08G12400 vs. TAIR 10
Match: AT4G32850.2 (nuclear poly(a) polymerase )

HSP 1 Score: 362.5 bits (929), Expect = 3.4e-100
Identity = 223/464 (48.06%), Postives = 248/464 (53.45%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAPN----YGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVG+ +  G  P  N    YG+TKP+SLAGP+ ADI+RN+ELEK+L+D GLYESK++  +
Sbjct: 2   MVGTQNLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMR 61

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVK+WVKQLT  RGYT+QMVEDANAVIFTFGSYRLGVHGPG+DIDTLCVG
Sbjct: 62  REEVLGRIDQIVKHWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVG 121

Query: 121 PSYVNR-EDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVP 180
           PSYVNR EDFFIILH+ILAEMEEV +L PVPDAHVPVM+FKF GI IDLLYASIS LVVP
Sbjct: 122 PSYVNREEDFFIILHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVP 181

Query: 181 EDLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE--------------------- 240
           +DLDIS  SVL  VDE TVRSLNGCRVADQIL+LVPN E                     
Sbjct: 182 QDLDISSSSVLCEVDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSN 241

Query: 241 VTGFLGGVNWALLVA---------------------------------------QLG--- 300
           VTGFLGGVNWALLVA                                       +LG   
Sbjct: 242 VTGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPV 301

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 302 WDRRKNHRDRYHLMPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWS 361

BLAST of Clc08G12400 vs. TAIR 10
Match: AT4G32850.3 (nuclear poly(a) polymerase )

HSP 1 Score: 362.5 bits (929), Expect = 3.4e-100
Identity = 223/464 (48.06%), Postives = 248/464 (53.45%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAPN----YGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVG+ +  G  P  N    YG+TKP+SLAGP+ ADI+RN+ELEK+L+D GLYESK++  +
Sbjct: 2   MVGTQNLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMR 61

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVK+WVKQLT  RGYT+QMVEDANAVIFTFGSYRLGVHGPG+DIDTLCVG
Sbjct: 62  REEVLGRIDQIVKHWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVG 121

Query: 121 PSYVNR-EDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVP 180
           PSYVNR EDFFIILH+ILAEMEEV +L PVPDAHVPVM+FKF GI IDLLYASIS LVVP
Sbjct: 122 PSYVNREEDFFIILHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVP 181

Query: 181 EDLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE--------------------- 240
           +DLDIS  SVL  VDE TVRSLNGCRVADQIL+LVPN E                     
Sbjct: 182 QDLDISSSSVLCEVDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSN 241

Query: 241 VTGFLGGVNWALLVA---------------------------------------QLG--- 300
           VTGFLGGVNWALLVA                                       +LG   
Sbjct: 242 VTGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPV 301

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 302 WDRRKNHRDRYHLMPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWS 361

BLAST of Clc08G12400 vs. TAIR 10
Match: AT4G32850.6 (nuclear poly(a) polymerase )

HSP 1 Score: 362.5 bits (929), Expect = 3.4e-100
Identity = 223/464 (48.06%), Postives = 248/464 (53.45%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAPN----YGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVG+ +  G  P  N    YG+TKP+SLAGP+ ADI+RN+ELEK+L+D GLYESK++  +
Sbjct: 2   MVGTQNLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMR 61

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVK+WVKQLT  RGYT+QMVEDANAVIFTFGSYRLGVHGPG+DIDTLCVG
Sbjct: 62  REEVLGRIDQIVKHWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVG 121

Query: 121 PSYVNR-EDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVP 180
           PSYVNR EDFFIILH+ILAEMEEV +L PVPDAHVPVM+FKF GI IDLLYASIS LVVP
Sbjct: 122 PSYVNREEDFFIILHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVP 181

Query: 181 EDLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE--------------------- 240
           +DLDIS  SVL  VDE TVRSLNGCRVADQIL+LVPN E                     
Sbjct: 182 QDLDISSSSVLCEVDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSN 241

Query: 241 VTGFLGGVNWALLVA---------------------------------------QLG--- 300
           VTGFLGGVNWALLVA                                       +LG   
Sbjct: 242 VTGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPV 301

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 302 WDRRKNHRDRYHLMPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWS 361

BLAST of Clc08G12400 vs. TAIR 10
Match: AT4G32850.5 (nuclear poly(a) polymerase )

HSP 1 Score: 362.5 bits (929), Expect = 3.4e-100
Identity = 223/464 (48.06%), Postives = 248/464 (53.45%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAPN----YGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVG+ +  G  P  N    YG+TKP+SLAGP+ ADI+RN+ELEK+L+D GLYESK++  +
Sbjct: 2   MVGTQNLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMR 61

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVK+WVKQLT  RGYT+QMVEDANAVIFTFGSYRLGVHGPG+DIDTLCVG
Sbjct: 62  REEVLGRIDQIVKHWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVG 121

Query: 121 PSYVNR-EDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVP 180
           PSYVNR EDFFIILH+ILAEMEEV +L PVPDAHVPVM+FKF GI IDLLYASIS LVVP
Sbjct: 122 PSYVNREEDFFIILHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVP 181

Query: 181 EDLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE--------------------- 240
           +DLDIS  SVL  VDE TVRSLNGCRVADQIL+LVPN E                     
Sbjct: 182 QDLDISSSSVLCEVDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSN 241

Query: 241 VTGFLGGVNWALLVA---------------------------------------QLG--- 300
           VTGFLGGVNWALLVA                                       +LG   
Sbjct: 242 VTGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPV 301

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 302 WDRRKNHRDRYHLMPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWS 361

BLAST of Clc08G12400 vs. TAIR 10
Match: AT4G32850.1 (nuclear poly(a) polymerase )

HSP 1 Score: 362.5 bits (929), Expect = 3.4e-100
Identity = 223/464 (48.06%), Postives = 248/464 (53.45%), Query Frame = 0

Query: 1   MVGSDSSPGFPPAPN----YGVTKPISLAGPTDADIQRNIELEKFLIDSGLYESKEEAAK 60
           MVG+ +  G  P  N    YG+TKP+SLAGP+ ADI+RN+ELEK+L+D GLYESK++  +
Sbjct: 2   MVGTQNLGGSLPPLNSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMR 61

Query: 61  REEVLGRIDQIVKNWVKQLTLLRGYTEQMVEDANAVIFTFGSYRLGVHGPGSDIDTLCVG 120
           REEVLGRIDQIVK+WVKQLT  RGYT+QMVEDANAVIFTFGSYRLGVHGPG+DIDTLCVG
Sbjct: 62  REEVLGRIDQIVKHWVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVG 121

Query: 121 PSYVNR-EDFFIILHNILAEMEEVADLQPVPDAHVPVMRFKFLGISIDLLYASISALVVP 180
           PSYVNR EDFFIILH+ILAEMEEV +L PVPDAHVPVM+FKF GI IDLLYASIS LVVP
Sbjct: 122 PSYVNREEDFFIILHDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVP 181

Query: 181 EDLDISHGSVLYNVDEQTVRSLNGCRVADQILRLVPNVE--------------------- 240
           +DLDIS  SVL  VDE TVRSLNGCRVADQIL+LVPN E                     
Sbjct: 182 QDLDISSSSVLCEVDEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSN 241

Query: 241 VTGFLGGVNWALLVA---------------------------------------QLG--- 300
           VTGFLGGVNWALLVA                                       +LG   
Sbjct: 242 VTGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPV 301

Query: 301 ------------------------------------------------------------ 305
                                                                       
Sbjct: 302 WDRRKNHRDRYHLMPIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWS 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132220.12.1e-12870.19nuclear poly(A) polymerase 2-like isoform X2 [Momordica charantia][more]
XP_038884467.11.2e-12357.45nuclear poly(A) polymerase 4-like isoform X3 [Benincasa hispida][more]
XP_008444997.12.8e-12056.59PREDICTED: nuclear poly(A) polymerase 4 isoform X3 [Cucumis melo][more]
XP_038884466.13.7e-12053.96nuclear poly(A) polymerase 4-like isoform X2 [Benincasa hispida][more]
XP_038884464.13.7e-12053.96nuclear poly(A) polymerase 4-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8VYW14.8e-9948.06Nuclear poly(A) polymerase 4 OS=Arabidopsis thaliana OX=3702 GN=PAPS4 PE=1 SV=1[more]
O823124.7e-9446.81Nuclear poly(A) polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=PAPS2 PE=1 SV=2[more]
Q9LMT23.4e-7659.66Nuclear poly(A) polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=PAPS1 PE=1 SV=1[more]
Q54J735.8e-6050.21Poly(A) polymerase OS=Dictyostelium discoideum OX=44689 GN=papA PE=3 SV=1[more]
P255006.8e-5342.74Poly(A) polymerase alpha OS=Bos taurus OX=9913 GN=PAPOLA PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1BRU61.0e-12870.19Polynucleotide adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC11100513... [more]
A0A1S3BBN81.4e-12056.59Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103488167 PE=3 ... [more]
A0A6J1K7X65.2e-12056.59Polynucleotide adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491975 P... [more]
A0A6J1HCH85.2e-12056.59Polynucleotide adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462377... [more]
A0A6J1KC705.2e-12056.59Polynucleotide adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491975 P... [more]
Match NameE-valueIdentityDescription
AT4G32850.23.4e-10048.06nuclear poly(a) polymerase [more]
AT4G32850.33.4e-10048.06nuclear poly(a) polymerase [more]
AT4G32850.63.4e-10048.06nuclear poly(a) polymerase [more]
AT4G32850.53.4e-10048.06nuclear poly(a) polymerase [more]
AT4G32850.13.4e-10048.06nuclear poly(a) polymerase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002934Polymerase, nucleotidyl transferase domainPFAMPF01909NTP_transf_2coord: 90..157
e-value: 2.1E-9
score: 37.6
IPR007012Poly(A) polymerase, central domainPFAMPF04928PAP_centralcoord: 17..214
e-value: 1.2E-31
score: 123.7
IPR043519Nucleotidyltransferase superfamilyGENE3D3.30.460.10Beta Polymerase, domain 2coord: 49..204
e-value: 2.4E-76
score: 258.5
IPR043519Nucleotidyltransferase superfamilySUPERFAMILY81301Nucleotidyltransferasecoord: 16..209
NoneNo IPR availableGENE3D1.10.1410.10coord: 17..230
e-value: 2.4E-76
score: 258.5
NoneNo IPR availablePANTHERPTHR10682:SF36NUCLEAR POLY(A) POLYMERASE 4coord: 1..231
NoneNo IPR availablePANTHERPTHR10682POLY A POLYMERASEcoord: 1..231
NoneNo IPR availablePANTHERPTHR10682POLY A POLYMERASEcoord: 236..304
NoneNo IPR availablePANTHERPTHR10682:SF36NUCLEAR POLY(A) POLYMERASE 4coord: 236..304
NoneNo IPR availableCDDcd05402NT_PAP_TUTasecoord: 56..174
e-value: 7.92006E-29
score: 104.947
IPR011068Nucleotidyltransferase, class I-like, C-terminalSUPERFAMILY55003PAP/Archaeal CCA-adding enzyme, C-terminal domaincoord: 236..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc08G12400.1Clc08G12400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031123 RNA 3'-end processing
biological_process GO:0043631 RNA polyadenylation
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0004652 polynucleotide adenylyltransferase activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0016779 nucleotidyltransferase activity