Clc08G05910 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc08G05910
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	Calcium-binding EF-hand family protein
Location	ClcChr08: 17226808 .. 17227431 (+)
RNA-Seq Expression	Clc08G05910
Synteny	Clc08G05910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAACCTGCAGCGGCTAATCTATCATAATTCAGTTGACAACAATAATTCCACACATTTCTTTTGTTATTAAACTTTCTTGTGTGTGTTATATTTCAATTCTAAACCAAGAACAAAAATCCATGGCTCCTATTAGCTCCAACGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAACGGCGATGGCCTCATTTGCTTGCAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGTTGGAGTCATTTCTAGAGAAACCGAGCCTCAACTTCGATGAATTCTTGTTCTTTTACGAGTCGATATCGAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGAGGGATGTGTGGAAGATGATGATCATGAGGAAGATATGGAGATTGTATATATGGCTTTCAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGTCGAGGCTCGAGCTTTGGGACGCAAATAGAAGCGATGTCGATTATTATAGAAGTATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCATATAGAGTTTC

mRNA sequence

AAAACCTGCAGCGGCTAATCTATCATAATTCAGTTGACAACAATAATTCCACACATTTCTTTTGTTATTAAACTTTCTTGTGTGTGTTATATTTCAATTCTAAACCAAGAACAAAAATCCATGGCTCCTATTAGCTCCAACGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAACGGCGATGGCCTCATTTGCTTGCAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGTTGGAGTCATTTCTAGAGAAACCGAGCCTCAACTTCGATGAATTCTTGTTCTTTTACGAGTCGATATCGAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGAGGGATGTGTGGAAGATGATGATCATGAGGAAGATATGGAGATTGTATATATGGCTTTCAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGTCGAGGCTCGAGCTTTGGGACGCAAATAGAAGCGATGTCGATTATTATAGAAGTATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCATATAGAGTTTC

Coding sequence (CDS)

ATGGCTCCTATTAGCTCCAACGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAACGGCGATGGCCTCATTTGCTTGCAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGTTGGAGTCATTTCTAGAGAAACCGAGCCTCAACTTCGATGAATTCTTGTTCTTTTACGAGTCGATATCGAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGAGGGATGTGTGGAAGATGATGATCATGAGGAAGATATGGAGATTGTATATATGGCTTTCAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGTCGAGGCTCGAGCTTTGGGACGCAAATAGAAGCGATGTCGATTATTATAGAAGTATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAG

Protein sequence

MAPISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFLFFYESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLTT

Homology

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BLAST of Clc08G05910 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 300.8 bits (769), Expect = 6.9e-78
Identity = 146/161 (90.68%), Postives = 154/161 (95.65%), Query Frame = 0


Query: 1   MAPISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFL 60
           M+ IS NDLHRIF KLDKNGDGLICLQELKWL DRIGIQLTMEELESFLEKPSL+F+EFL
Sbjct: 11  MSSISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFL 70

Query: 61  FFYESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRL 120
           FFYESISKQNKGECKGG GC++ DDHEE+MEIVYMAFKVFDMNGDGFISCDELENVLSRL
Sbjct: 71  FFYESISKQNKGECKGGVGCLQ-DDHEEEMEIVYMAFKVFDMNGDGFISCDELENVLSRL 130

Query: 121 ELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDANR+D+DY RSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 131 ELWDANRNDIDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 170

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BLAST of Clc08G05910 vs. NCBI nr
Match: XP_008441608.1 (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 putative calcium-binding protein CML44 [Cucumis melo var. makuwa])

HSP 1 Score: 294.3 bits (752), Expect = 6.5e-76
Identity = 146/162 (90.12%), Postives = 154/162 (95.06%), Query Frame = 0


Query: 1   MAPISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFL 60
           M+PISS DLHRIFKKLDKN DGL+CLQELKWLLDRIGIQLTMEELESFLEKPSL+FDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGG-EGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSR 120
           FFYESISKQNKGECKGG  GCV+ DDHE+D EIVYMAFKVFDMNGDGFISCDELENVL R
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQ-DDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVR 120

Query: 121 LELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLTT 162
           LELWDA+RS+VDY RSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

[Close]

BLAST of Clc08G05910 vs. NCBI nr
Match: XP_004149438.1 (probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetical protein Csa_018262 [Cucumis sativus])

HSP 1 Score: 287.7 bits (735), Expect = 6.1e-74
Identity = 143/165 (86.67%), Postives = 153/165 (92.73%), Query Frame = 0


Query: 1   MAPISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFL 60
           M PISS DLHRIFKKLDKN DGLICLQEL WLLD IGIQLTMEELESFLE+PSL+FDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGG-EGCVEDDDH---EEDMEIVYMAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGG  GCV+D+D+   ++DMEIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LSRLELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLTT 162
           L +LELWDA+RSDVDY RSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

[Close]

BLAST of Clc08G05910 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 265.8 bits (678), Expect = 2.5e-67
Identity = 136/158 (86.08%), Postives = 143/158 (90.51%), Query Frame = 0


Query: 3   PISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFLFF 62
           PISSNDL RIF +LDKN DGLIC+QELKWLLD IGIQLTMEELESFLEKPSL+FDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLEL 122
           YESISKQNKGECKGG    E  D EED+EIVYMAFKVFD+NGDGFISC+ELENVLSRLEL
Sbjct: 64  YESISKQNKGECKGGL-VDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLT 161
           WDANRS+VD  RSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of Clc08G05910 vs. NCBI nr
Match: XP_022993348.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 265.4 bits (677), Expect = 3.2e-67
Identity = 134/158 (84.81%), Postives = 144/158 (91.14%), Query Frame = 0


Query: 3   PISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFLFF 62
           PISSNDL RIF +LDKNGDGLIC+QELKWLLD IGIQLTMEELESFLEKPSL+FDEFL F
Sbjct: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63

Query: 63  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLEL 122
           YES+SKQNKGECKGG    E +D +ED+EIVYMAFKVFD+NGDGFISC+ELENVLSRLEL
Sbjct: 64  YESMSKQNKGECKGGL-VDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLT 161
           WDANRS+VD  RSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of Clc08G05910 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 9.5e-30
Identity = 76/162 (46.91%), Postives = 100/162 (61.73%), Query Frame = 0


Query: 4   ISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIG-IQLTMEELESFLEKPSLNFDEFLFF 63
           I++NDL R+FK LDKN DGL+ L EL W+LD++G  + T +ELE  + K SL+ DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRL-- 123
           Y      +KG  K  +   ++D      E +  AF VFD+NGDG+IS +EL +VL RL  
Sbjct: 66  YYDAVLDSKGSKKNIDVVADND------EAIARAFNVFDVNGDGYISAEELRDVLERLGF 125

Query: 124 ----ELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMML 159
               + WD  R        MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 EEEAKAWDCGR--------MIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of Clc08G05910 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.6e-21
Identity = 63/156 (40.38%), Postives = 88/156 (56.41%), Query Frame = 0


Query: 9   LHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLE------KPSLNFDEFLFF 68
           L RIF   DKNGDG I ++EL   L R+G+   + +L+S +E         LNFD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKQ-NKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLE 128
           ++++      G C GGE   +     E+   +  AFKVFD NGDGFIS  EL+ VL +L 
Sbjct: 90  HKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLG 149

Query: 129 LWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMM 158
           L +    +++    MI + D N DG++DF EFKNMM
Sbjct: 150 LPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of Clc08G05910 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.7e-21
Identity = 62/155 (40.00%), Postives = 89/155 (57.42%), Query Frame = 0


Query: 9   LHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFL------EKPSLNFDEFLFF 68
           LHR+F   DKN DG I ++EL   L R+G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLEL 128
           ++++ +       GGEG   D   E D+E    AF VFD +GDGFIS  EL+ VL +L L
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMM 158
            +A   +++    MI + D+N DG++DF EFKNMM
Sbjct: 149 PEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of Clc08G05910 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 4.4e-19
Identity = 66/168 (39.29%), Postives = 87/168 (51.79%), Query Frame = 0


Query: 4   ISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFD------ 63
           + S +L ++FK  DKNGDG I  +EL       GI +  +EL++ ++K   N D      
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFLFFYESI--------SKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFIS 123
           EF   Y SI        + +      GGEG   DD+ E   E    AF VFD NGDGFI+
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMRE----AFNVFDQNGDGFIT 120

Query: 124 CDELENVLSRLELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMM 158
            DEL +VLS L L   +    D  R MI   D + DG++DF+EFK MM
Sbjct: 121 VDELRSVLSSLGL--KHGRTADDCRRMISMVDADGDGRVDFKEFKQMM 162

[Close]

BLAST of Clc08G05910 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 7.6e-19
Identity = 61/164 (37.20%), Postives = 95/164 (57.93%), Query Frame = 0


Query: 9   LHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLE------KPSLNFDEFLFF 68
           L RIF   DKN DG+I + EL   L+ +G++  + ELES ++         L F++F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMA---------FKVFDMNGDGFISCDEL 128
           ++S++  +     GGE   ++DD+EEDM    ++         FKVFD +GDG+IS  EL
Sbjct: 101 HQSLN--DSYFAYGGE---DEDDNEEDMRKSILSQEEADSFGGFKVFDEDGDGYISAREL 160

Query: 129 ENVLSRLELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMM 158
           + VL +L    +  S++D    MI + D+N DG++DF EFK+MM
Sbjct: 161 QMVLGKLGF--SEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMM 197

[Close]

BLAST of Clc08G05910 vs. ExPASy TrEMBL
Match: A0A5D3B6U1 (Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold282G00080 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.1e-76
Identity = 146/162 (90.12%), Postives = 154/162 (95.06%), Query Frame = 0


Query: 1   MAPISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFL 60
           M+PISS DLHRIFKKLDKN DGL+CLQELKWLLDRIGIQLTMEELESFLEKPSL+FDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGG-EGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSR 120
           FFYESISKQNKGECKGG  GCV+ DDHE+D EIVYMAFKVFDMNGDGFISCDELENVL R
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQ-DDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVR 120

Query: 121 LELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLTT 162
           LELWDA+RS+VDY RSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

[Close]

BLAST of Clc08G05910 vs. ExPASy TrEMBL
Match: A0A1S3B3T2 (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.1e-76
Identity = 146/162 (90.12%), Postives = 154/162 (95.06%), Query Frame = 0


Query: 1   MAPISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFL 60
           M+PISS DLHRIFKKLDKN DGL+CLQELKWLLDRIGIQLTMEELESFLEKPSL+FDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGG-EGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSR 120
           FFYESISKQNKGECKGG  GCV+ DDHE+D EIVYMAFKVFDMNGDGFISCDELENVL R
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQ-DDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVR 120

Query: 121 LELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLTT 162
           LELWDA+RS+VDY RSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

[Close]

BLAST of Clc08G05910 vs. ExPASy TrEMBL
Match: A0A0A0KFQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.9e-74
Identity = 143/165 (86.67%), Postives = 153/165 (92.73%), Query Frame = 0


Query: 1   MAPISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFL 60
           M PISS DLHRIFKKLDKN DGLICLQEL WLLD IGIQLTMEELESFLE+PSL+FDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGG-EGCVEDDDH---EEDMEIVYMAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGG  GCV+D+D+   ++DMEIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LSRLELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLTT 162
           L +LELWDA+RSDVDY RSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

[Close]

BLAST of Clc08G05910 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.6e-67
Identity = 134/158 (84.81%), Postives = 144/158 (91.14%), Query Frame = 0


Query: 3   PISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFLFF 62
           PISSNDL RIF +LDKNGDGLIC+QELKWLLD IGIQLTMEELESFLEKPSL+FDEFL F
Sbjct: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63

Query: 63  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLEL 122
           YES+SKQNKGECKGG    E +D +ED+EIVYMAFKVFD+NGDGFISC+ELENVLSRLEL
Sbjct: 64  YESMSKQNKGECKGGL-VDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLT 161
           WDANRS+VD  RSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of Clc08G05910 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.7e-67
Identity = 135/158 (85.44%), Postives = 143/158 (90.51%), Query Frame = 0


Query: 3   PISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFDEFLFF 62
           PISSNDL RIF +LDKN DGLIC+QELKWLLD IGIQLTMEELESFLEKPSL+FDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLEL 122
           YESISKQNKGECKGG    E  D EED+EIVYMAFKVFD+NGDGFISC+ELENVLSRLEL
Sbjct: 64  YESISKQNKGECKGGL-VDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMMLLT 161
           WDAN+S+VD  RSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of Clc08G05910 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 131.3 bits (329), Expect = 6.8e-31
Identity = 76/162 (46.91%), Postives = 100/162 (61.73%), Query Frame = 0


Query: 4   ISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIG-IQLTMEELESFLEKPSLNFDEFLFF 63
           I++NDL R+FK LDKN DGL+ L EL W+LD++G  + T +ELE  + K SL+ DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRL-- 123
           Y      +KG  K  +   ++D      E +  AF VFD+NGDG+IS +EL +VL RL  
Sbjct: 66  YYDAVLDSKGSKKNIDVVADND------EAIARAFNVFDVNGDGYISAEELRDVLERLGF 125

Query: 124 ----ELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMML 159
               + WD  R        MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 EEEAKAWDCGR--------MIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of Clc08G05910 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 104.0 bits (258), Expect = 1.2e-22
Identity = 63/156 (40.38%), Postives = 88/156 (56.41%), Query Frame = 0


Query: 9   LHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLE------KPSLNFDEFLFF 68
           L RIF   DKNGDG I ++EL   L R+G+   + +L+S +E         LNFD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKQ-NKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLE 128
           ++++      G C GGE   +     E+   +  AFKVFD NGDGFIS  EL+ VL +L 
Sbjct: 90  HKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLG 149

Query: 129 LWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMM 158
           L +    +++    MI + D N DG++DF EFKNMM
Sbjct: 150 LPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of Clc08G05910 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 102.4 bits (254), Expect = 3.4e-22
Identity = 62/155 (40.00%), Postives = 89/155 (57.42%), Query Frame = 0


Query: 9   LHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFL------EKPSLNFDEFLFF 68
           LHR+F   DKN DG I ++EL   L R+G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLEL 128
           ++++ +       GGEG   D   E D+E    AF VFD +GDGFIS  EL+ VL +L L
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMM 158
            +A   +++    MI + D+N DG++DF EFKNMM
Sbjct: 149 PEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of Clc08G05910 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-18
Identity = 60/156 (38.46%), Postives = 86/156 (55.13%), Query Frame = 0


Query: 8   DLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLN------FDEFLF 67
           +L R+F+  DKNGDG I  +EL   L  +GI +  +EL   +EK  +N       DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 68  FYESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVLSRLE 127
            Y++I               E+D+ EEDM+    AF VFD NGDGFI+ DEL+ VLS L 
Sbjct: 65  LYKTIMD-------------EEDEEEEDMK---EAFNVFDQNGDGFITVDELKAVLSSLG 124

Query: 128 LWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMM 158
           L       +D  + MI+  D + DG+++++EF+ MM
Sbjct: 125 L--KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of Clc08G05910 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 85.1 bits (209), Expect = 5.6e-17
Identity = 58/160 (36.25%), Postives = 87/160 (54.37%), Query Frame = 0


Query: 4   ISSNDLHRIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLNFD------ 63
           + S +L+R+F+  DK+GDG I  +EL      +GI +  +EL   ++K  +N D      
Sbjct: 1   MDSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIE 60

Query: 64  EFLFFYESISKQNKGECKGGEGCVEDDDHEEDMEIVYMAFKVFDMNGDGFISCDELENVL 123
           EF   Y++I  ++           ED+  EEDM+    AF VFD NGDGFI+ DEL+ VL
Sbjct: 61  EFGELYKTIMVED-----------EDEVGEEDMK---EAFNVFDRNGDGFITVDELKAVL 120

Query: 124 SRLELWDANRSDVDYYRSMIRAYDTNLDGKLDFEEFKNMM 158
           S L L       ++  R MI   D + DG++++ EF+ MM
Sbjct: 121 SSLGL--KQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMM 144

The following BLAST results are available for this feature:

BLAST of Clc08G05910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885742.1	6.9e-78	90.68	probable calcium-binding protein CML44 [Benincasa hispida]	[more]
XP_008441608.1	6.5e-76	90.12	PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 put...	[more]
XP_004149438.1	6.1e-74	86.67	probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetica...	[more]
XP_023551499.1	2.5e-67	86.08	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]
XP_022993348.1	3.2e-67	84.81	probable calcium-binding protein CML44 [Cucurbita maxima]	[more]

BLAST of Clc08G05910 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	9.5e-30	46.91	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q9SVG9	1.6e-21	40.38	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q9FI19	4.7e-21	40.00	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q2QVI1	4.4e-19	39.29	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
P43187	7.6e-19	37.20	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]

BLAST of Clc08G05910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3B6U1	3.1e-76	90.12	Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B3T2	3.1e-76	90.12	probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P...	[more]
A0A0A0KFQ6	2.9e-74	86.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1	[more]
A0A6J1K1Y7	1.6e-67	84.81	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893...	[more]
A0A6J1FLU5	2.7e-67	85.44	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of Clc08G05910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	6.8e-31	46.91	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	1.2e-22	40.38	calmodulin like 42	[more]
AT5G44460.1	3.4e-22	40.00	calmodulin like 43	[more]
AT1G05990.1	1.0e-18	38.46	EF hand calcium-binding protein family	[more]
AT4G03290.1	5.6e-17	36.25	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 132..160 e-value: 0.0082 score: 25.4 coord: 92..120 e-value: 0.011 score: 24.9 coord: 8..36 e-value: 1.2E-4 score: 31.5
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 8..33 e-value: 3.7E-6 score: 26.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 94..157 e-value: 1.4E-10 score: 41.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 4..39 score: 12.979158
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..123 score: 13.007053
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 128..161 score: 13.537066
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 8..60 e-value: 1.71032E-8 score: 46.7721
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 96..157 e-value: 3.70612E-12 score: 56.4021
None	No IPR available	GENE3D	1.10.238.10		coord: 69..160 e-value: 1.1E-17 score: 66.3
None	No IPR available	GENE3D	1.10.238.10		coord: 5..68 e-value: 1.1E-10 score: 43.4
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 3..160
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 3..160
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 17..29
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 7..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc08G05910.1	Clc08G05910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding