Clc08G03715 (gene) Watermelon (cordophanus) v2

Overview
NameClc08G03715
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionPectin lyase-like superfamily protein
LocationClcChr08: 10610522 .. 10611053 (+)
RNA-Seq ExpressionClc08G03715
SyntenyClc08G03715
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGTTCAGAGAGAAAGCGGGAAATGTTGAGAGAGAAAGGGAAAGGTTCTGAGAGAAAGCGAAAGCAGAAAAGTTTTCCAACGGGTAGAAGGCGACTTTTAAAGAAAAAATGGAAATGCGCGTTTTGGGCTGTTTTAATTTTGGGGGGTAAAATTGGTATTTCATGTGGCCAAAAGGTCATTTATGATAAAGTTTTTGGGAGTGGGTCATCCATAGATAAGTTTTTAGTTTTTGGGTCATTTCGTGCAATTTTCCAGTTAGGGAATACTTTTTTTTAAAAAAAAGAAATTGTTGTTGGTATGGAACAGACTTCATGAATTAAAATTAGCGACGTGACATACCAAAACATTCATGGAACATCAGCTACAAAAGTTGCAGTAAAATTTGATTGTAGTGCAACAAATCCGTGCAATCGAATAAAGTTGGAGTATGTAAAGCTTAGTTATATGAATCAAATAGCCCAAGCAACGTGCAACAATGTTGGAGGGATTGCAACAGGTCTTGTTCAGCCTACTAGTTGCTTTTAG

mRNA sequence

ATGGGGAACGACGTGACATACCAAAACATTCATGGAACATCAGCTACAAAAGTTGCAGTAAAATTTGATTGTAGTGCAACAAATCCGTGCAATCGAATAAAGTTGGAGTATGTAAAGCTTAGTTATATGAATCAAATAGCCCAAGCAACGTGCAACAATGTTGGAGGGATTGCAACAGGTCTTGTTCAGCCTACTAGTTGCTTTTAG

Coding sequence (CDS)

ATGGGGAACGACGTGACATACCAAAACATTCATGGAACATCAGCTACAAAAGTTGCAGTAAAATTTGATTGTAGTGCAACAAATCCGTGCAATCGAATAAAGTTGGAGTATGTAAAGCTTAGTTATATGAATCAAATAGCCCAAGCAACGTGCAACAATGTTGGAGGGATTGCAACAGGTCTTGTTCAGCCTACTAGTTGCTTTTAG

Protein sequence

MGNDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLVQPTSCF
Homology
BLAST of Clc08G03715 vs. NCBI nr
Match: XP_011652823.2 (polygalacturonase [Cucumis sativus] >KAE8650851.1 hypothetical protein Csa_001451 [Cucumis sativus])

HSP 1 Score: 122.1 bits (305), Expect = 1.9e-24
Identity = 58/66 (87.88%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATKVAVKFDCS  NPC RIKLE VKL+YMNQ AQATCNNVGGIA GLV
Sbjct: 334 NDVTYQDIHGTSATKVAVKFDCSPINPCVRIKLENVKLTYMNQTAQATCNNVGGIAAGLV 393

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 394 QPTSCF 399

BLAST of Clc08G03715 vs. NCBI nr
Match: XP_008460449.1 (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 121.3 bits (303), Expect = 3.2e-24
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATK+AVKFDCS TNPC  IKLE V+L+YMNQ AQATCNNVGGIA GLV
Sbjct: 219 NDVTYQDIHGTSATKIAVKFDCSPTNPCGGIKLENVRLTYMNQTAQATCNNVGGIAAGLV 278

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 279 QPTSCF 284

BLAST of Clc08G03715 vs. NCBI nr
Match: KAA0068064.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 121.3 bits (303), Expect = 3.2e-24
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATK+AVKFDCS TNPC  IKLE V+L+YMNQ AQATCNNVGGIA GLV
Sbjct: 335 NDVTYQDIHGTSATKIAVKFDCSPTNPCGGIKLENVRLTYMNQTAQATCNNVGGIAAGLV 394

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 395 QPTSCF 400

BLAST of Clc08G03715 vs. NCBI nr
Match: TYK06443.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 121.3 bits (303), Expect = 3.2e-24
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATK+AVKFDCS TNPC  IKLE V+L+YMNQ AQATCNNVGGIA GLV
Sbjct: 335 NDVTYQDIHGTSATKIAVKFDCSPTNPCGGIKLENVRLTYMNQTAQATCNNVGGIAAGLV 394

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 395 QPTSCF 400

BLAST of Clc08G03715 vs. NCBI nr
Match: XP_011652824.2 (polygalacturonase [Cucumis sativus] >KAE8650860.1 hypothetical protein Csa_002650 [Cucumis sativus])

HSP 1 Score: 120.9 bits (302), Expect = 4.1e-24
Identity = 57/66 (86.36%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATKVAVKFDCS  NPC RIKL+ VKL+YMNQ AQATCNNVGGIA GLV
Sbjct: 334 NDVTYQDIHGTSATKVAVKFDCSPINPCVRIKLDNVKLTYMNQTAQATCNNVGGIAAGLV 393

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 394 QPTSCF 399

BLAST of Clc08G03715 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.6e-18
Identity = 41/65 (63.08%), Postives = 53/65 (81.54%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           +DVTY++IHGTSAT+VAVKFDCS  +PC  IKLE VKL+Y NQ A+++C++  G   G+V
Sbjct: 328 SDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTYKNQAAESSCSHADGTTEGVV 387

Query: 63  QPTSC 68
           QPTSC
Sbjct: 388 QPTSC 392

BLAST of Clc08G03715 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 8.7e-09
Identity = 29/65 (44.62%), Postives = 42/65 (64.62%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           ++V Y++IHGTS T+VAV  DCS   PC  I ++ V L  +++ AQA+C+N  G A  +V
Sbjct: 333 SNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSANDVV 392

Query: 63  QPTSC 68
             T C
Sbjct: 393 PFTPC 397

BLAST of Clc08G03715 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 2.8e-07
Identity = 31/62 (50.00%), Postives = 36/62 (58.06%), Query Frame = 0

Query: 4   DVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYM-NQIAQATCNNVGGIATGLV 63
           +V YQNI GT A+ VA+ FDCS   PC  I LE V L       A+A CNNV    TG+V
Sbjct: 392 NVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVELSETGVV 451

Query: 64  QP 65
            P
Sbjct: 452 SP 453

BLAST of Clc08G03715 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 2.8e-07
Identity = 27/52 (51.92%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 4   DVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKL-SYMNQIAQATCNNV 55
           +V Y+NI GTSATKVA+KFDCS   PC  I +E + L     + ++ATC NV
Sbjct: 379 NVVYENIKGTSATKVAIKFDCSTNFPCEGIIMENINLVGESGKPSEATCKNV 430

BLAST of Clc08G03715 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.8e-06
Identity = 26/67 (38.81%), Postives = 37/67 (55.22%), Query Frame = 0

Query: 4   DVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSY--MNQIAQATCNNVGGIATGL 63
           DVT++NI GTS+T  AV   C+A  PC  + ++ V + Y   N    A C N  G A G 
Sbjct: 344 DVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKGSAKGC 403

Query: 64  VQPTSCF 69
           ++  +CF
Sbjct: 404 LKELACF 410

BLAST of Clc08G03715 vs. ExPASy TrEMBL
Match: A0A0A0LCF2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G634290 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 9.0e-25
Identity = 58/66 (87.88%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATKVAVKFDCS  NPC RIKLE VKL+YMNQ AQATCNNVGGIA GLV
Sbjct: 87  NDVTYQDIHGTSATKVAVKFDCSPINPCVRIKLENVKLTYMNQTAQATCNNVGGIAAGLV 146

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 147 QPTSCF 152

BLAST of Clc08G03715 vs. ExPASy TrEMBL
Match: A0A5A7VKX0 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00120 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-24
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATK+AVKFDCS TNPC  IKLE V+L+YMNQ AQATCNNVGGIA GLV
Sbjct: 335 NDVTYQDIHGTSATKIAVKFDCSPTNPCGGIKLENVRLTYMNQTAQATCNNVGGIAAGLV 394

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 395 QPTSCF 400

BLAST of Clc08G03715 vs. ExPASy TrEMBL
Match: A0A5D3C3E8 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G001500 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-24
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATK+AVKFDCS TNPC  IKLE V+L+YMNQ AQATCNNVGGIA GLV
Sbjct: 335 NDVTYQDIHGTSATKIAVKFDCSPTNPCGGIKLENVRLTYMNQTAQATCNNVGGIAAGLV 394

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 395 QPTSCF 400

BLAST of Clc08G03715 vs. ExPASy TrEMBL
Match: A0A1S3CCL4 (polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103499261 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-24
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATK+AVKFDCS TNPC  IKLE V+L+YMNQ AQATCNNVGGIA GLV
Sbjct: 219 NDVTYQDIHGTSATKIAVKFDCSPTNPCGGIKLENVRLTYMNQTAQATCNNVGGIAAGLV 278

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 279 QPTSCF 284

BLAST of Clc08G03715 vs. ExPASy TrEMBL
Match: A0A5D3DTW1 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold876G00270 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.0e-23
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDVTYQ+IHGTSATKVAVKFDCS TNPC  IKLE V+L++MNQ AQATCNNVGGIA GLV
Sbjct: 335 NDVTYQDIHGTSATKVAVKFDCSPTNPCVGIKLENVRLTHMNQTAQATCNNVGGIAAGLV 394

Query: 63  QPTSCF 69
           QPTSCF
Sbjct: 395 QPTSCF 400

BLAST of Clc08G03715 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 73.9 bits (180), Expect = 5.4e-14
Identity = 33/62 (53.23%), Postives = 43/62 (69.35%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           +DV + +IHGTSAT+V VK DCS+  PC  I+LE VKL+Y N+ A + C + GGI  G  
Sbjct: 320 SDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQNKPAASACTHAGGIEAGFF 379

Query: 63  QP 65
           QP
Sbjct: 380 QP 381

BLAST of Clc08G03715 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 3.5e-13
Identity = 31/65 (47.69%), Postives = 44/65 (67.69%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           +DV + +IHGTSAT+V VK DCS+  PC  I+++ VKL+Y N+ A   C++ GG   G  
Sbjct: 323 SDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLTYRNKPATTDCSHAGGSEAGFQ 382

Query: 63  QPTSC 68
           +P SC
Sbjct: 383 RPNSC 387

BLAST of Clc08G03715 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 6.0e-13
Identity = 29/65 (44.62%), Postives = 43/65 (66.15%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           + V Y+NI GTS T+ A+ FDCS +NPC  I+L  +KL++  + A +TC N+ G+  G+V
Sbjct: 327 SQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLHDIKLTFNGRSATSTCKNIKGVKAGVV 386

Query: 63  QPTSC 68
            P  C
Sbjct: 387 MPQGC 391

BLAST of Clc08G03715 vs. TAIR 10
Match: AT1G65570.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 5.1e-12
Identity = 29/65 (44.62%), Postives = 41/65 (63.08%), Query Frame = 0

Query: 3   NDVTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLV 62
           NDV Y  I GTSAT++A+K DCS   PC  I+++ + L+   + A+ +C NV G   GLV
Sbjct: 332 NDVIYSGIMGTSATEIAIKMDCSEKVPCTGIRMQAINLTSYGEAAKTSCTNVSGKQLGLV 391

Query: 63  QPTSC 68
            P+ C
Sbjct: 392 TPSGC 396

BLAST of Clc08G03715 vs. TAIR 10
Match: AT2G43880.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 2.5e-11
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 0

Query: 5   VTYQNIHGTSATKVAVKFDCSATNPCNRIKLEYVKLSYMNQIAQATCNNVGGIATGLVQP 64
           VT+ NI GTS T +A+K DCS +N C  ++L+ +KL+YM + + + C N  G A+G++ P
Sbjct: 331 VTFANIKGTSTTPIAMKLDCSGSNHCTGLRLQDIKLTYMRRSSASYCRNAHGRASGVMVP 390

Query: 65  TSC 68
            +C
Sbjct: 391 RNC 393

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011652823.21.9e-2487.88polygalacturonase [Cucumis sativus] >KAE8650851.1 hypothetical protein Csa_00145... [more]
XP_008460449.13.2e-2484.85PREDICTED: polygalacturonase-like [Cucumis melo][more]
KAA0068064.13.2e-2484.85polygalacturonase [Cucumis melo var. makuwa][more]
TYK06443.13.2e-2484.85polygalacturonase [Cucumis melo var. makuwa][more]
XP_011652824.24.1e-2486.36polygalacturonase [Cucumis sativus] >KAE8650860.1 hypothetical protein Csa_00265... [more]
Match NameE-valueIdentityDescription
P489791.6e-1863.08Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228188.7e-0944.62Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
P353362.8e-0750.00Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
P051172.8e-0751.92Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1[more]
P353391.8e-0638.81Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LCF29.0e-2587.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G634290 PE=3 SV=1[more]
A0A5A7VKX01.5e-2484.85Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00... [more]
A0A5D3C3E81.5e-2484.85Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00... [more]
A0A1S3CCL41.5e-2484.85polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103499261 PE=3 SV=1[more]
A0A5D3DTW11.0e-2384.85Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold876G00... [more]
Match NameE-valueIdentityDescription
AT2G43870.15.4e-1453.23Pectin lyase-like superfamily protein [more]
AT3G59850.13.5e-1347.69Pectin lyase-like superfamily protein [more]
AT2G43890.16.0e-1344.62Pectin lyase-like superfamily protein [more]
AT1G65570.15.1e-1244.62Pectin lyase-like superfamily protein [more]
AT2G43880.12.5e-1142.86Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 2..67
e-value: 2.1E-11
score: 45.3
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 3..55
e-value: 2.6E-8
score: 33.3
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 3..67
NoneNo IPR availablePANTHERPTHR31375:SF247POLYGALACTURONASE-LIKEcoord: 3..67
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 3..68

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc08G03715.1Clc08G03715.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004650 polygalacturonase activity