Clc08G02275 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc08G02275
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	RING/U-box superfamily protein
Location	ClcChr08: 4332194 .. 4332394 (-)
RNA-Seq Expression	Clc08G02275
Synteny	Clc08G02275

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGAGAACTATGATGGGATTGGGCGGGAAGATGAGGTGGGAAATCAGTGCAGTGTTTGTTTTGAGGATCTCAACGGGGAAAAAGAAAAGGAGGTGGTCAAAATACCCTGCGGGCATGTGTATCGTAAGTCATGTATTCTCAAATGGCTTAACATCAACAATTCATGTCCTTTGTGTAGAAGGGAATTAGAAGGATAA

mRNA sequence

ATGAAGGAGAACTATGATGGGATTGGGCGGGAAGATGAGGTGGGAAATCAGTGCAGTGTTTGTTTTGAGGATCTCAACGGGGAAAAAGAAAAGGAGGTGGTCAAAATACCCTGCGGGCATGTGTATCGTAAGTCATGTATTCTCAAATGGCTTAACATCAACAATTCATGTCCTTTGTGTAGAAGGGAATTAGAAGGATAA

Coding sequence (CDS)

ATGAAGGAGAACTATGATGGGATTGGGCGGGAAGATGAGGTGGGAAATCAGTGCAGTGTTTGTTTTGAGGATCTCAACGGGGAAAAAGAAAAGGAGGTGGTCAAAATACCCTGCGGGCATGTGTATCGTAAGTCATGTATTCTCAAATGGCTTAACATCAACAATTCATGTCCTTTGTGTAGAAGGGAATTAGAAGGATAA

Protein sequence

MKENYDGIGREDEVGNQCSVCFEDLNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRRELEG

Homology

[Close]

BLAST of Clc08G02275 vs. NCBI nr
Match: XP_031740500.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucumis sativus])

HSP 1 Score: 102.1 bits (253), Expect = 1.9e-18
Identity = 49/72 (68.06%), Postives = 56/72 (77.78%), Query Frame = 0


Query: 1   MKENYDGIGREDEVG-NQCSVCFEDLNG------EKEKEVVKIPCGHVYRKSCILKWLNI 60
           MKE YDG G E++ G +QCSVC+E L G      EKEKEV +IPCGH+Y KSCILKWL I
Sbjct: 159 MKEKYDGNGGEEKEGDDQCSVCYEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKI 218

Query: 61  NNSCPLCRRELE 66
           +NSCPLCRRELE
Sbjct: 219 SNSCPLCRRELE 230

[Close]

BLAST of Clc08G02275 vs. NCBI nr
Match: XP_011650219.1 (RING-H2 finger protein ATL16 [Cucumis sativus] >KGN63362.1 hypothetical protein Csa_022479 [Cucumis sativus])

HSP 1 Score: 100.9 bits (250), Expect = 4.3e-18
Identity = 47/67 (70.15%), Postives = 56/67 (83.58%), Query Frame = 0


Query: 1   MKENYDGIGREDEVGNQCSVCFEDLNG--EKEKEVVKIPCGHVYRKSCILKWLNINNSCP 60
           MKE YDG G E E+ ++CSVC+EDL+G  EKEKEV +IPCGH++ KSCILKWL I+NSCP
Sbjct: 156 MKEKYDGNGGE-EMEDECSVCYEDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCP 215

Query: 61  LCRRELE 66
           LCR ELE
Sbjct: 216 LCRGELE 221

[Close]

BLAST of Clc08G02275 vs. NCBI nr
Match: XP_008466247.1 (PREDICTED: E3 ubiquitin-protein ligase synoviolin B-like [Cucumis melo] >KAA0038718.1 E3 ubiquitin-protein ligase synoviolin B-like [Cucumis melo var. makuwa] >TYK31330.1 E3 ubiquitin-protein ligase synoviolin B-like [Cucumis melo var. makuwa])

HSP 1 Score: 99.4 bits (246), Expect = 1.3e-17
Identity = 45/67 (67.16%), Postives = 51/67 (76.12%), Query Frame = 0


Query: 1   MKENYDGIGREDEVGNQCSVCFEDLN----GEKEKEVVKIPCGHVYRKSCILKWLNINNS 60
           M E YD     +E+G+QC VC+EDLN     EKE+EV +IPCGHVY K CILKWLNINNS
Sbjct: 150 MDEKYDDGNGREEMGDQCCVCYEDLNCEREEEKEEEVTRIPCGHVYHKYCILKWLNINNS 209

Query: 61  CPLCRRE 64
           CPLCRRE
Sbjct: 210 CPLCRRE 216

[Close]

BLAST of Clc08G02275 vs. NCBI nr
Match: XP_011652885.1 (RING-H2 finger protein ATL66 [Cucumis sativus] >KGN60202.1 hypothetical protein Csa_002222 [Cucumis sativus])

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-15
Identity = 41/66 (62.12%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 1   MKENYDGIGREDEVGNQCSVCFEDLNGEKE---KEVVKIPCGHVYRKSCILKWLNINNSC 60
           M E YD     + +G QC VC+EDLN E+E   +E  +IPCGHVY KSCILKWLN+NNSC
Sbjct: 148 MNEKYDDGNGGEGMGGQCCVCYEDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSC 207

Query: 61  PLCRRE 64
           PLCR E
Sbjct: 208 PLCRSE 213

[Close]

BLAST of Clc08G02275 vs. NCBI nr
Match: XP_008442291.1 (PREDICTED: RING finger protein 165-like [Cucumis melo] >KAA0041218.1 RING finger protein 165-like protein [Cucumis melo var. makuwa] >TYK16021.1 RING finger protein 165-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 91.3 bits (225), Expect = 3.4e-15
Identity = 42/67 (62.69%), Postives = 52/67 (77.61%), Query Frame = 0


Query: 1   MKENYDGIGR--EDEVGNQCSVCFEDLNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCP 60
           MK+ +DG  +  E+E+G+ CSVC+E+L G K KEV +IPCGHVY KSC+L WL INNSCP
Sbjct: 99  MKQKFDGFNKEEEEEIGD-CSVCYEELKG-KGKEVSRIPCGHVYHKSCVLTWLQINNSCP 158

Query: 61  LCRRELE 66
           LCR  LE
Sbjct: 159 LCRAPLE 163

[Close]

BLAST of Clc08G02275 vs. ExPASy Swiss-Prot
Match: Q9Y7K6 (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC2A9.04c PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 5.3e-11
Identity = 22/62 (35.48%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 3   ENYDGIGREDEVGNQCSVCFEDLNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRR 62
           ++++ +  +  +   C +C++D+N   EK+  K+PCGH++ K+C+ KWL  + +CPLCR+
Sbjct: 92  DSFEPLSNDQLMDLTCPICYDDMNENDEKQATKMPCGHIFGKNCLQKWLENHCTCPLCRK 151

Query: 63  EL 65
           E+
Sbjct: 152 EV 153

[Close]

BLAST of Clc08G02275 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.4e-10
Identity = 25/49 (51.02%), Postives = 35/49 (71.43%), Query Frame = 0


Query: 16  NQCSVCFEDLNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRREL 65
           NQC+VC +D   E+  E  ++PC H+Y K C+L WL ++NSCP+CR EL
Sbjct: 222 NQCAVCMDDF--EEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHEL 268

[Close]

BLAST of Clc08G02275 vs. ExPASy Swiss-Prot
Match: Q9VHI7 (E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.9e-09
Identity = 21/48 (43.75%), Postives = 38/48 (79.17%), Query Frame = 0


Query: 17  QCSVCFEDLNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRREL 65
           QCS+C++D   + ++ V K+PC H+Y ++CI+ WLN++++CP+CR+ L
Sbjct: 252 QCSICWDDF--KIDETVRKLPCSHLYHENCIVPWLNLHSTCPICRKSL 297

[Close]

BLAST of Clc08G02275 vs. ExPASy Swiss-Prot
Match: Q9Y4L5 (E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 5.0e-09
Identity = 23/54 (42.59%), Postives = 37/54 (68.52%), Query Frame = 0


Query: 13  EVGNQCSVCFEDLNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRRELEG 67
           ++G +C VC ED     E+EV ++PC H +  SCI+ WL ++++CP+CR+ L G
Sbjct: 223 DMGLECPVCKEDYT--VEEEVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNG 274

[Close]

BLAST of Clc08G02275 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 8.5e-09
Identity = 23/49 (46.94%), Postives = 35/49 (71.43%), Query Frame = 0


Query: 16  NQCSVCFEDLNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRREL 65
           NQC+VC ++   E   +V ++PC HV+ + C+L WL ++NSCP+CR EL
Sbjct: 214 NQCAVCMDEF--EDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

[Close]

BLAST of Clc08G02275 vs. ExPASy TrEMBL
Match: A0A0A0L3Z2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 9.4e-19
Identity = 49/72 (68.06%), Postives = 56/72 (77.78%), Query Frame = 0


Query: 1   MKENYDGIGREDEVG-NQCSVCFEDLNG------EKEKEVVKIPCGHVYRKSCILKWLNI 60
           MKE YDG G E++ G +QCSVC+E L G      EKEKEV +IPCGH+Y KSCILKWL I
Sbjct: 159 MKEKYDGNGGEEKEGDDQCSVCYEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKI 218

Query: 61  NNSCPLCRRELE 66
           +NSCPLCRRELE
Sbjct: 219 SNSCPLCRRELE 230

[Close]

BLAST of Clc08G02275 vs. ExPASy TrEMBL
Match: A0A0A0LRB0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.1e-18
Identity = 47/67 (70.15%), Postives = 56/67 (83.58%), Query Frame = 0


Query: 1   MKENYDGIGREDEVGNQCSVCFEDLNG--EKEKEVVKIPCGHVYRKSCILKWLNINNSCP 60
           MKE YDG G E E+ ++CSVC+EDL+G  EKEKEV +IPCGH++ KSCILKWL I+NSCP
Sbjct: 156 MKEKYDGNGGE-EMEDECSVCYEDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCP 215

Query: 61  LCRRELE 66
           LCR ELE
Sbjct: 216 LCRGELE 221

[Close]

BLAST of Clc08G02275 vs. ExPASy TrEMBL
Match: A0A5A7T784 (E3 ubiquitin-protein ligase synoviolin B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006300 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 6.1e-18
Identity = 45/67 (67.16%), Postives = 51/67 (76.12%), Query Frame = 0


Query: 1   MKENYDGIGREDEVGNQCSVCFEDLN----GEKEKEVVKIPCGHVYRKSCILKWLNINNS 60
           M E YD     +E+G+QC VC+EDLN     EKE+EV +IPCGHVY K CILKWLNINNS
Sbjct: 150 MDEKYDDGNGREEMGDQCCVCYEDLNCEREEEKEEEVTRIPCGHVYHKYCILKWLNINNS 209

Query: 61  CPLCRRE 64
           CPLCRRE
Sbjct: 210 CPLCRRE 216

[Close]

BLAST of Clc08G02275 vs. ExPASy TrEMBL
Match: A0A1S3CR16 (E3 ubiquitin-protein ligase synoviolin B-like OS=Cucumis melo OX=3656 GN=LOC103503717 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 6.1e-18
Identity = 45/67 (67.16%), Postives = 51/67 (76.12%), Query Frame = 0


Query: 1   MKENYDGIGREDEVGNQCSVCFEDLN----GEKEKEVVKIPCGHVYRKSCILKWLNINNS 60
           M E YD     +E+G+QC VC+EDLN     EKE+EV +IPCGHVY K CILKWLNINNS
Sbjct: 150 MDEKYDDGNGREEMGDQCCVCYEDLNCEREEEKEEEVTRIPCGHVYHKYCILKWLNINNS 209

Query: 61  CPLCRRE 64
           CPLCRRE
Sbjct: 210 CPLCRRE 216

[Close]

BLAST of Clc08G02275 vs. ExPASy TrEMBL
Match: A0A0A0LHI3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889100 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 5.7e-16
Identity = 41/66 (62.12%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 1   MKENYDGIGREDEVGNQCSVCFEDLNGEKE---KEVVKIPCGHVYRKSCILKWLNINNSC 60
           M E YD     + +G QC VC+EDLN E+E   +E  +IPCGHVY KSCILKWLN+NNSC
Sbjct: 148 MNEKYDDGNGGEGMGGQCCVCYEDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSC 207

Query: 61  PLCRRE 64
           PLCR E
Sbjct: 208 PLCRSE 213

[Close]

BLAST of Clc08G02275 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.6e-10
Identity = 24/51 (47.06%), Postives = 36/51 (70.59%), Query Frame = 0


Query: 16  NQCSVCFED--LNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRREL 65
           +QC+VC E+  + G    +  ++PC H+Y K CI+ WL +NNSCP+CRR+L
Sbjct: 222 SQCTVCMEEFIVGG----DATELPCKHIYHKDCIVPWLRLNNSCPICRRDL 268

[Close]

BLAST of Clc08G02275 vs. TAIR 10
Match: AT4G12190.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 4.6e-10
Identity = 22/48 (45.83%), Postives = 33/48 (68.75%), Query Frame = 0


Query: 18 CSVCFEDL-NGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRREL 65
          CS+C E L +G K +++ ++ C HV+   C+L+WL   N+CPLCR EL
Sbjct: 22 CSICLESLVSGPKPRDITRMTCSHVFHNGCLLEWLKRKNTCPLCRTEL 69

[Close]

BLAST of Clc08G02275 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 6.0e-10
Identity = 23/49 (46.94%), Postives = 35/49 (71.43%), Query Frame = 0


Query: 16  NQCSVCFEDLNGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRREL 65
           NQC+VC ++   E   +V ++PC HV+ + C+L WL ++NSCP+CR EL
Sbjct: 214 NQCAVCMDEF--EDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

[Close]

BLAST of Clc08G02275 vs. TAIR 10
Match: AT4G05350.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 6.0e-10
Identity = 22/48 (45.83%), Postives = 33/48 (68.75%), Query Frame = 0


Query: 18  CSVCFEDL-NGEKEKEVVKIPCGHVYRKSCILKWLNINNSCPLCRREL 65
           CS+C E L +G K ++V ++ C HV+   C+L+WL   N+CPLCR E+
Sbjct: 157 CSICLESLVSGPKPRDVTRMTCSHVFHNGCLLEWLKRKNTCPLCRTEI 204

[Close]

BLAST of Clc08G02275 vs. TAIR 10
Match: AT5G37230.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 3.0e-09
Identity = 23/52 (44.23%), Postives = 32/52 (61.54%), Query Frame = 0


Query: 12  DEVGNQCSVCFEDLNGEKEKEVVKIP-CGHVYRKSCILKWLNINNSCPLCRR 63
           DE    CS+C ED +   +  ++ +P C H++ +SCI KWL    SCPLCRR
Sbjct: 147 DEEETTCSICMEDFSESHDDNIILLPDCFHLFHQSCIFKWLKRQRSCPLCRR 198

The following BLAST results are available for this feature:

BLAST of Clc08G02275 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031740500.1	1.9e-18	68.06	E3 ubiquitin-protein ligase At1g63170-like [Cucumis sativus]	[more]
XP_011650219.1	4.3e-18	70.15	RING-H2 finger protein ATL16 [Cucumis sativus] >KGN63362.1 hypothetical protein ...	[more]
XP_008466247.1	1.3e-17	67.16	PREDICTED: E3 ubiquitin-protein ligase synoviolin B-like [Cucumis melo] >KAA0038...	[more]
XP_011652885.1	1.2e-15	62.12	RING-H2 finger protein ATL66 [Cucumis sativus] >KGN60202.1 hypothetical protein ...	[more]
XP_008442291.1	3.4e-15	62.69	PREDICTED: RING finger protein 165-like [Cucumis melo] >KAA0041218.1 RING finger...	[more]

BLAST of Clc08G02275 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y7K6	5.3e-11	35.48	Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai...	[more]
P0CH30	3.4e-10	51.02	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9VHI7	2.9e-09	43.75	E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1...	[more]
Q9Y4L5	5.0e-09	42.59	E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2	[more]
Q8LPN7	8.5e-09	46.94	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]

BLAST of Clc08G02275 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3Z2	9.4e-19	68.06	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G551140 P...	[more]
A0A0A0LRB0	2.1e-18	70.15	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432790 P...	[more]
A0A5A7T784	6.1e-18	67.16	E3 ubiquitin-protein ligase synoviolin B-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3CR16	6.1e-18	67.16	E3 ubiquitin-protein ligase synoviolin B-like OS=Cucumis melo OX=3656 GN=LOC1035...	[more]
A0A0A0LHI3	5.7e-16	62.12	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889100 P...	[more]

BLAST of Clc08G02275 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60360.1	1.6e-10	47.06	RING/U-box superfamily protein	[more]
AT4G12190.1	4.6e-10	45.83	RING/U-box superfamily protein	[more]
AT3G19950.1	6.0e-10	46.94	RING/U-box superfamily protein	[more]
AT4G05350.1	6.0e-10	45.83	RING/U-box superfamily protein	[more]
AT5G37230.1	3.0e-09	44.23	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 18..60 e-value: 2.7E-5 score: 33.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 16..61 e-value: 2.1E-13 score: 50.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 18..61 score: 12.813844
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 6..66 e-value: 7.9E-19 score: 68.6
None	No IPR available	PANTHER	PTHR45931:SF9	E3 UBIQUITIN-PROTEIN LIGASE RNF12-B-LIKE	coord: 11..65
None	No IPR available	PANTHER	PTHR45931	SI:CH211-59O9.10	coord: 11..65
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 13..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc08G02275.1	Clc08G02275.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity