Clc08G02270 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc08G02270
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	E3 ubiquitin-protein ligase RNF126-like
Location	ClcChr08: 4328337 .. 4329005 (-)
RNA-Seq Expression	Clc08G02270
Synteny	Clc08G02270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTCCGTAACACTCTGTCCCTTCGATATTGGATCGGCAGCGACCCCAACGGCAGCGTCGCCACCGACGATCAACCATTGCAATTCGTCCAACTTCGATTGGGGAGTTTCAGTGAATTAGTGCTTGCACCTTCTCACCAAATCCTCCAACAAACTTCCCCAACTCTAATTTCCGATGCTCTGTTTCCATTCTCTCTGCGGGAGCTTGAAGACCCACCATTCCTCGCCCACTACTTCCTCCTTCGTTTTCTCTCTTCCTTCAACCTCTCCGCCGCCGCCTGTGACGCCATCTTCCACAAGATCGCCTCCTTCGCCCTCCGAATGGCCGCCGGAAACCTGAATCTCAACTTCCACATCATCGCCGCTGTGGATTTGGTACACACGATCTGGGTGGACCTGGACCCAGCGCTGCTTCTTCCACAAGATTCGGAGCCGGTGAGGAAAGGGGCGCCGGCGTCGGTGATGGAGCGGTTAATGAAGGAGAAGTATGATGGGAATGGGCGGGAAGAGACGGGAGATCAGTGCAGCGTTTGTTACGAGGATCTCCATGGGGAAAAGGAGTTGGCAAGAATCCCCTGTGGACATGTGTATCATAAGCCCTGCATCCTCAAATGGCTTAACATCAACAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

mRNA sequence

ATGTCTTTCCGTAACACTCTGTCCCTTCGATATTGGATCGGCAGCGACCCCAACGGCAGCGTCGCCACCGACGATCAACCATTGCAATTCGTCCAACTTCGATTGGGGAGTTTCAGTGAATTAGTGCTTGCACCTTCTCACCAAATCCTCCAACAAACTTCCCCAACTCTAATTTCCGATGCTCTGTTTCCATTCTCTCTGCGGGAGCTTGAAGACCCACCATTCCTCGCCCACTACTTCCTCCTTCGTTTTCTCTCTTCCTTCAACCTCTCCGCCGCCGCCTGTGACGCCATCTTCCACAAGATCGCCTCCTTCGCCCTCCGAATGGCCGCCGGAAACCTGAATCTCAACTTCCACATCATCGCCGCTGTGGATTTGGTACACACGATCTGGGTGGACCTGGACCCAGCGCTGCTTCTTCCACAAGATTCGGAGCCGGTGAGGAAAGGGGCGCCGGCGTCGGTGATGGAGCGGTTAATGAAGGAGAAGTATGATGGGAATGGGCGGGAAGAGACGGGAGATCAGTGCAGCGTTTGTTACGAGGATCTCCATGGGGAAAAGGAGTTGGCAAGAATCCCCTGTGGACATGTGTATCATAAGCCCTGCATCCTCAAATGGCTTAACATCAACAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

Coding sequence (CDS)

ATGTCTTTCCGTAACACTCTGTCCCTTCGATATTGGATCGGCAGCGACCCCAACGGCAGCGTCGCCACCGACGATCAACCATTGCAATTCGTCCAACTTCGATTGGGGAGTTTCAGTGAATTAGTGCTTGCACCTTCTCACCAAATCCTCCAACAAACTTCCCCAACTCTAATTTCCGATGCTCTGTTTCCATTCTCTCTGCGGGAGCTTGAAGACCCACCATTCCTCGCCCACTACTTCCTCCTTCGTTTTCTCTCTTCCTTCAACCTCTCCGCCGCCGCCTGTGACGCCATCTTCCACAAGATCGCCTCCTTCGCCCTCCGAATGGCCGCCGGAAACCTGAATCTCAACTTCCACATCATCGCCGCTGTGGATTTGGTACACACGATCTGGGTGGACCTGGACCCAGCGCTGCTTCTTCCACAAGATTCGGAGCCGGTGAGGAAAGGGGCGCCGGCGTCGGTGATGGAGCGGTTAATGAAGGAGAAGTATGATGGGAATGGGCGGGAAGAGACGGGAGATCAGTGCAGCGTTTGTTACGAGGATCTCCATGGGGAAAAGGAGTTGGCAAGAATCCCCTGTGGACATGTGTATCATAAGCCCTGCATCCTCAAATGGCTTAACATCAACAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

Protein sequence

MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISDALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHIIAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCYEDLHGEKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE

Homology

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BLAST of Clc08G02270 vs. NCBI nr
Match: XP_011650219.1 (RING-H2 finger protein ATL16 [Cucumis sativus] >KGN63362.1 hypothetical protein Csa_022479 [Cucumis sativus])

HSP 1 Score: 306.6 bits (784), Expect = 1.7e-79
Identity = 154/225 (68.44%), Postives = 178/225 (79.11%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           MS   T S R  I  + NGS AT+ QPLQFV+LRLG FS+LVL+PSHQILQQTSP LISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHI 120
            L  F LRELEDPPF++H  L RFLSS+N+S A CDAIF KI+SFA ++ AGNLN NFHI
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120

Query: 121 IAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCY 180
           IAA+D V+T WVDLDPA    +D+  VR+GAP S +ERLMKEKYDGNG EE  D+CSVCY
Sbjct: 121 IAALDFVYTHWVDLDPA----EDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCY 180

Query: 181 EDLHG----EKELARIPCGHVYHKPCILKWLNINNSCPLCRRELE 222
           EDLHG    EKE++RIPCGH++HK CILKWL I+NSCPLCR ELE
Sbjct: 181 EDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 221

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BLAST of Clc08G02270 vs. NCBI nr
Match: XP_031740500.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucumis sativus])

HSP 1 Score: 287.3 bits (734), Expect = 1.1e-73
Identity = 151/235 (64.26%), Postives = 177/235 (75.32%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           MS   T S +  I  +   S+ATD QPLQFVQLRL  F +LV  PS QILQQTSPTLISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFA---LRMAAGNLNLN 120
            L PFSL ELEDPPF++++FL RFLSS+++SAA CDAIF+ I+SFA   L  A  N+N+N
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREET--GDQ 180
           FHIIA VD+V+T WVDLDPA    +D+  VRKGA  SV+ER+MKEKYDGNG EE    DQ
Sbjct: 121 FHIIADVDIVYTHWVDLDPA----EDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQ 180

Query: 181 CSVCYEDLHG--------EKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           CSVCYE L+G        EKE++RIPCGH+YHK CILKWL I+NSCPLCRRELEE
Sbjct: 181 CSVCYEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE 231

[Close]

BLAST of Clc08G02270 vs. NCBI nr
Match: XP_023006824.1 (RING-H2 finger protein ATL16-like [Cucurbita maxima])

HSP 1 Score: 251.5 bits (641), Expect = 6.6e-63
Identity = 120/222 (54.05%), Postives = 164/222 (73.87%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           ++F ++LS ++ I ++  G++A+ + P+QFV+LRLG  S L+   S QILQQ+  T + +
Sbjct: 3   LTFGDSLSFQFSIRTNTTGTLASHEHPMQFVELRLGHSSHLISRSSRQILQQSPSTPLGE 62

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHI 120
           +LFPF LR+LE PPFL +Y +L FLSSF++  AACD I+ +I+SFALR+ A N++LNFHI
Sbjct: 63  SLFPFILRDLEHPPFLPNYHVLHFLSSFSIPVAACDIIYQEISSFALRLTADNVDLNFHI 122

Query: 121 IAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCY 180
           +  VD +  IWVDLDP  L+ + S P   GAPAS +ERL ++K+DG   EE    CSVC 
Sbjct: 123 VVDVDAIEMIWVDLDP-FLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEEGDCSVCC 182

Query: 181 EDLHGEKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           E L GE+E++RIPCGHVYHK CILKWL I+NSCPLCR +LE+
Sbjct: 183 EALRGEEEVSRIPCGHVYHKCCILKWLQISNSCPLCRTKLEQ 223

[Close]

BLAST of Clc08G02270 vs. NCBI nr
Match: XP_022947933.1 (E3 ubiquitin-protein ligase RNF126-like [Cucurbita moschata] >XP_023532920.1 E3 ubiquitin-protein ligase RNF126-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250.8 bits (639), Expect = 1.1e-62
Identity = 121/222 (54.50%), Postives = 167/222 (75.23%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           ++F + LS +  I S  NG+VA+D QP+Q ++LRLG+ + L+   S QILQQT  T + D
Sbjct: 3   LTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPMGD 62

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHI 120
           ALFPF LR+L+ PPFL++ ++L+FLSSF++  AACD I+ +I+SFAL++AAGN++LNFHI
Sbjct: 63  ALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNFHI 122

Query: 121 IAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCY 180
           +  VD++  IWVDLDP  L+ + S     GAPAS +ERL ++++DG   EE    CSVC 
Sbjct: 123 VVEVDVIEMIWVDLDP-FLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCC 182

Query: 181 EDLHGEKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           E L GE+E++RIPCGHVYHK CILKWL ++NSCPLCR +LE+
Sbjct: 183 EALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of Clc08G02270 vs. NCBI nr
Match: KAG6605221.1 (E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 250.0 bits (637), Expect = 1.9e-62
Identity = 121/222 (54.50%), Postives = 167/222 (75.23%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           ++F + LS +  I S  NG+VA+D QP+Q ++LRLG+ + L+   S QILQQT  T + D
Sbjct: 3   LTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPMGD 62

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHI 120
           ALFPF LR+L+ PPFL++ ++L+FLSSF++  AACD I+ +I+SFAL++AAGN++LNFHI
Sbjct: 63  ALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNFHI 122

Query: 121 IAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCY 180
           +  VD++  IWVDLDP  L+ + S     GAPAS +ERL ++++DG   EE    CSVC 
Sbjct: 123 VVEVDVIEMIWVDLDP-FLVEEYSPAEMNGAPASAIERLQRQEFDGLRDEEEEGDCSVCC 182

Query: 181 EDLHGEKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           E L GE+E++RIPCGHVYHK CILKWL ++NSCPLCR +LE+
Sbjct: 183 EALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of Clc08G02270 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.1e-11
Identity = 31/81 (38.27%), Postives = 46/81 (56.79%), Query Frame = 0


Query: 143 DSEPVRKGAP---ASVMERLMKEKYDGNGREETGDQCSVCYEDLHGEKELARIPCGHVYH 202
           +++P R G P    S +E L       +      +QC+VC +D     E  ++PC H+YH
Sbjct: 188 ENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYH 247

Query: 203 KPCILKWLNINNSCPLCRREL 221
           K C+L WL ++NSCP+CR EL
Sbjct: 248 KDCLLPWLELHNSCPVCRHEL 268

[Close]

BLAST of Clc08G02270 vs. ExPASy Swiss-Prot
Match: Q9VHI7 (E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 20/48 (41.67%), Postives = 38/48 (79.17%), Query Frame = 0


Query: 175 QCSVCYEDLHGEKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           QCS+C++D   ++ + ++PC H+YH+ CI+ WLN++++CP+CR+ L +
Sbjct: 252 QCSICWDDFKIDETVRKLPCSHLYHENCIVPWLNLHSTCPICRKSLAD 299

[Close]

BLAST of Clc08G02270 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-10
Identity = 28/81 (34.57%), Postives = 49/81 (60.49%), Query Frame = 0


Query: 143 DSEPVRKGAP---ASVMERLMKEKYDGNGREETGDQCSVCYEDLHGEKELARIPCGHVYH 202
           +++P R G P    S ++ L   K   +  +   +QC+VC ++     ++ ++PC HV+H
Sbjct: 180 ENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFH 239

Query: 203 KPCILKWLNINNSCPLCRREL 221
           + C+L WL ++NSCP+CR EL
Sbjct: 240 QDCLLPWLELHNSCPVCRFEL 260

[Close]

BLAST of Clc08G02270 vs. ExPASy Swiss-Prot
Match: Q9DBU5 (E3 ubiquitin-protein ligase RNF6 OS=Mus musculus OX=10090 GN=Rnf6 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.4e-09
Identity = 32/86 (37.21%), Postives = 48/86 (55.81%), Query Frame = 0


Query: 138 LLLPQDSEPVRKGAPASVMERLMKEKYDGNGRE-ETGDQCSVCYEDLHGEKELARIPCGH 197
           LL   D +P+R G     ++ L    Y+ +G + E G  CSVC  D     +L ++PC H
Sbjct: 576 LLNEGDDDPIR-GLTKEQIDNLSTRSYEQDGVDSELGKVCSVCISDYVAGNKLRQLPCLH 635

Query: 198 VYHKPCILKWLNINNSCPLCRRELEE 223
            +H  CI +WL+ N +CP+CRR + E
Sbjct: 636 EFHIHCIDRWLSENCTCPVCRRPVLE 660

[Close]

BLAST of Clc08G02270 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.4e-09
Identity = 26/70 (37.14%), Postives = 40/70 (57.14%), Query Frame = 0


Query: 151 APASVMERLMKEKYDGNGREETGDQCSVCYEDLHGEKELARIPCGHVYHKPCILKWLNIN 210
           AP S ++ L   K      + +   C VC ++   + E  ++PC H+YH  CI+ WL  +
Sbjct: 161 APKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQH 220

Query: 211 NSCPLCRREL 221
           NSCP+CR+EL
Sbjct: 221 NSCPVCRKEL 230

[Close]

BLAST of Clc08G02270 vs. ExPASy TrEMBL
Match: A0A0A0LRB0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 8.4e-80
Identity = 154/225 (68.44%), Postives = 178/225 (79.11%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           MS   T S R  I  + NGS AT+ QPLQFV+LRLG FS+LVL+PSHQILQQTSP LISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHI 120
            L  F LRELEDPPF++H  L RFLSS+N+S A CDAIF KI+SFA ++ AGNLN NFHI
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120

Query: 121 IAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCY 180
           IAA+D V+T WVDLDPA    +D+  VR+GAP S +ERLMKEKYDGNG EE  D+CSVCY
Sbjct: 121 IAALDFVYTHWVDLDPA----EDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCY 180

Query: 181 EDLHG----EKELARIPCGHVYHKPCILKWLNINNSCPLCRRELE 222
           EDLHG    EKE++RIPCGH++HK CILKWL I+NSCPLCR ELE
Sbjct: 181 EDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 221

[Close]

BLAST of Clc08G02270 vs. ExPASy TrEMBL
Match: A0A0A0L3Z2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 5.3e-74
Identity = 151/235 (64.26%), Postives = 177/235 (75.32%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           MS   T S +  I  +   S+ATD QPLQFVQLRL  F +LV  PS QILQQTSPTLISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFA---LRMAAGNLNLN 120
            L PFSL ELEDPPF++++FL RFLSS+++SAA CDAIF+ I+SFA   L  A  N+N+N
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREET--GDQ 180
           FHIIA VD+V+T WVDLDPA    +D+  VRKGA  SV+ER+MKEKYDGNG EE    DQ
Sbjct: 121 FHIIADVDIVYTHWVDLDPA----EDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQ 180

Query: 181 CSVCYEDLHG--------EKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           CSVCYE L+G        EKE++RIPCGH+YHK CILKWL I+NSCPLCRRELEE
Sbjct: 181 CSVCYEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE 231

[Close]

BLAST of Clc08G02270 vs. ExPASy TrEMBL
Match: A0A6J1L387 (RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111499493 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 3.2e-63
Identity = 120/222 (54.05%), Postives = 164/222 (73.87%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           ++F ++LS ++ I ++  G++A+ + P+QFV+LRLG  S L+   S QILQQ+  T + +
Sbjct: 3   LTFGDSLSFQFSIRTNTTGTLASHEHPMQFVELRLGHSSHLISRSSRQILQQSPSTPLGE 62

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHI 120
           +LFPF LR+LE PPFL +Y +L FLSSF++  AACD I+ +I+SFALR+ A N++LNFHI
Sbjct: 63  SLFPFILRDLEHPPFLPNYHVLHFLSSFSIPVAACDIIYQEISSFALRLTADNVDLNFHI 122

Query: 121 IAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCY 180
           +  VD +  IWVDLDP  L+ + S P   GAPAS +ERL ++K+DG   EE    CSVC 
Sbjct: 123 VVDVDAIEMIWVDLDP-FLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEEGDCSVCC 182

Query: 181 EDLHGEKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           E L GE+E++RIPCGHVYHK CILKWL I+NSCPLCR +LE+
Sbjct: 183 EALRGEEEVSRIPCGHVYHKCCILKWLQISNSCPLCRTKLEQ 223

[Close]

BLAST of Clc08G02270 vs. ExPASy TrEMBL
Match: A0A6J1G7Z5 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC111451666 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 5.5e-63
Identity = 121/222 (54.50%), Postives = 167/222 (75.23%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           ++F + LS +  I S  NG+VA+D QP+Q ++LRLG+ + L+   S QILQQT  T + D
Sbjct: 3   LTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPMGD 62

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHI 120
           ALFPF LR+L+ PPFL++ ++L+FLSSF++  AACD I+ +I+SFAL++AAGN++LNFHI
Sbjct: 63  ALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNFHI 122

Query: 121 IAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCY 180
           +  VD++  IWVDLDP  L+ + S     GAPAS +ERL ++++DG   EE    CSVC 
Sbjct: 123 VVEVDVIEMIWVDLDP-FLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCC 182

Query: 181 EDLHGEKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           E L GE+E++RIPCGHVYHK CILKWL ++NSCPLCR +LE+
Sbjct: 183 EALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of Clc08G02270 vs. ExPASy TrEMBL
Match: A0A6J1KYV1 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499496 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 4.6e-62
Identity = 122/222 (54.95%), Postives = 164/222 (73.87%), Query Frame = 0


Query: 1   MSFRNTLSLRYWIGSDPNGSVATDDQPLQFVQLRLGSFSELVLAPSHQILQQTSPTLISD 60
           ++F + LS +  I S  NG+VA+  QP+Q ++LRLG  + L+   S QILQQT  T I D
Sbjct: 3   LTFHDYLSFQCSIQSTCNGTVASGQQPIQLLELRLGHSTHLISRSSRQILQQTPSTPIGD 62

Query: 61  ALFPFSLRELEDPPFLAHYFLLRFLSSFNLSAAACDAIFHKIASFALRMAAGNLNLNFHI 120
           ALFPF LR+L+ PPFL++ ++L+FLSSF++  AACD I+ +I+SFAL++AAGN++LNFHI
Sbjct: 63  ALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNMDLNFHI 122

Query: 121 IAAVDLVHTIWVDLDPALLLPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCY 180
           +  VD+   IWVDLDP  L+ + S     GAPAS +ERL ++K+DG   EE    CSVC 
Sbjct: 123 VVEVDVNEMIWVDLDP-FLVEEYSPAEMNGAPASAIERLQRQKFDGLREEEEEGDCSVCC 182

Query: 181 EDLHGEKELARIPCGHVYHKPCILKWLNINNSCPLCRRELEE 223
           E L GE+E++RIPCGHVYHK CILKWL ++NSCPLCR +LE+
Sbjct: 183 EALKGEEEVSRIPCGHVYHKFCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of Clc08G02270 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.3e-12
Identity = 31/81 (38.27%), Postives = 41/81 (50.62%), Query Frame = 0


Query: 140 LPQDSEPVRKGAPASVMERLMKEKYDGNGREETGDQCSVCYEDLHGEKELARIPCGHVYH 199
           L QD  P    A    +  L   K           QC+VC E+     +   +PC H+YH
Sbjct: 188 LTQDDRPGPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYH 247

Query: 200 KPCILKWLNINNSCPLCRREL 221
           K CI+ WL +NNSCP+CRR+L
Sbjct: 248 KDCIVPWLRLNNSCPICRRDL 268

[Close]

BLAST of Clc08G02270 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-11
Identity = 28/81 (34.57%), Postives = 49/81 (60.49%), Query Frame = 0


Query: 143 DSEPVRKGAP---ASVMERLMKEKYDGNGREETGDQCSVCYEDLHGEKELARIPCGHVYH 202
           +++P R G P    S ++ L   K   +  +   +QC+VC ++     ++ ++PC HV+H
Sbjct: 180 ENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFH 239

Query: 203 KPCILKWLNINNSCPLCRREL 221
           + C+L WL ++NSCP+CR EL
Sbjct: 240 QDCLLPWLELHNSCPVCRFEL 260

[Close]

BLAST of Clc08G02270 vs. TAIR 10
Match: AT3G02340.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-10
Identity = 33/96 (34.38%), Postives = 52/96 (54.17%), Query Frame = 0


Query: 128 HTIWVDLDPALLLPQDSEPVRKGAP---ASVMERLMKEKYDGNGREETGDQCSVCYEDLH 187
           H+   D D  L    D E   +G P    SV++ L   +      ++  + C+VC +++ 
Sbjct: 284 HSGHYDADAILGQMFDDETGIRGNPPAAKSVIQDLPVVELAVEELDKGNNVCAVCKDEML 343

Query: 188 GEKELARIPCGHVYHKPCILKWLNINNSCPLCRREL 221
            E+++ R+PC H YH  CI+ WL I N+CP+CR EL
Sbjct: 344 VEEKVRRLPCSHFYHGECIIPWLGIRNTCPVCRYEL 379

[Close]

BLAST of Clc08G02270 vs. TAIR 10
Match: AT5G67120.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-10
Identity = 29/84 (34.52%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 138 LLLPQDSEPVRKGAPASVM-ERLMKEKYDGNGREETGDQCSVCYEDLHGEKELARIPCGH 197
           L +  ++   R G    V+ E L++ KY+    +ET  +C +C + L   +E++++ CGH
Sbjct: 189 LSITDETGDERTGLSEEVIDENLIRRKYEKRSDDET-KRCVICQQKLKDNEEVSKLGCGH 248

Query: 198 VYHKPCILKWLNINNSCPLCRREL 221
            +H  CI  WL + N CPLC RE+
Sbjct: 249 DFHFGCIKNWLMVTNKCPLCNREV 271

[Close]

BLAST of Clc08G02270 vs. TAIR 10
Match: AT5G37200.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-10
Identity = 28/84 (33.33%), Postives = 49/84 (58.33%), Query Frame = 0


Query: 138 LLLPQDSEPVRKGAPASVMERLMKE-KYDGNGREETGDQCSVCYEDL-HGEKELARIP-C 197
           ++ P     + +GA   V  RL++E + +    EE  + CS+C E      +++ R+P C
Sbjct: 120 VIFPHGPSLLSRGASGEVFHRLVEEQRVESADLEEEDETCSICIEKFSESHEDIIRVPDC 179

Query: 198 GHVYHKPCILKWLNINNSCPLCRR 219
            H++H+ C+ +WL + NSCPLCR+
Sbjct: 180 LHLFHQGCLFEWLGLQNSCPLCRK 203

The following BLAST results are available for this feature:

BLAST of Clc08G02270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650219.1	1.7e-79	68.44	RING-H2 finger protein ATL16 [Cucumis sativus] >KGN63362.1 hypothetical protein ...	[more]
XP_031740500.1	1.1e-73	64.26	E3 ubiquitin-protein ligase At1g63170-like [Cucumis sativus]	[more]
XP_023006824.1	6.6e-63	54.05	RING-H2 finger protein ATL16-like [Cucurbita maxima]	[more]
XP_022947933.1	1.1e-62	54.50	E3 ubiquitin-protein ligase RNF126-like [Cucurbita moschata] >XP_023532920.1 E3 ...	[more]
KAG6605221.1	1.9e-62	54.50	E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Clc08G02270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CH30	2.1e-11	38.27	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9VHI7	1.8e-10	41.67	E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1...	[more]
Q8LPN7	3.0e-10	34.57	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9DBU5	3.4e-09	37.21	E3 ubiquitin-protein ligase RNF6 OS=Mus musculus OX=10090 GN=Rnf6 PE=1 SV=1	[more]
Q94AK4	3.4e-09	37.14	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]

BLAST of Clc08G02270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRB0	8.4e-80	68.44	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432790 P...	[more]
A0A0A0L3Z2	5.3e-74	64.26	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G551140 P...	[more]
A0A6J1L387	3.2e-63	54.05	RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111499493 PE...	[more]
A0A6J1G7Z5	5.5e-63	54.50	E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KYV1	4.6e-62	54.95	E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499...	[more]

BLAST of Clc08G02270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60360.1	3.3e-12	38.27	RING/U-box superfamily protein	[more]
AT3G19950.1	2.2e-11	34.57	RING/U-box superfamily protein	[more]
AT3G02340.1	1.1e-10	34.38	RING/U-box superfamily protein	[more]
AT5G67120.1	1.1e-10	34.52	RING/U-box superfamily protein	[more]
AT5G37200.1	1.1e-10	33.33	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 176..216 e-value: 1.6E-7 score: 41.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 174..217 e-value: 2.4E-13 score: 50.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 176..217 score: 13.271187
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 152..222 e-value: 6.1E-21 score: 75.4
None	No IPR available	PANTHER	PTHR45931	SI:CH211-59O9.10	coord: 79..221
None	No IPR available	PANTHER	PTHR45931:SF9	E3 UBIQUITIN-PROTEIN LIGASE RNF12-B-LIKE	coord: 79..221
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 175..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc08G02270.1	Clc08G02270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity