Clc06G17220 (gene) Watermelon (cordophanus) v2

BLAST of Clc06G17220 vs. NCBI nr
Match: XP_038880150.1 (protein NEN1-like [Benincasa hispida])

HSP 1 Score: 866.3 bits (2237), Expect = 1.5e-247
Identity = 456/510 (89.41%), Postives = 469/510 (91.96%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           M   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI
Sbjct: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           SSLSVRCNGITRDAVISSPTFA IADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEPKGTIDSLALLTQRFGRRAGDMK+ T  S      +    H          SLDDVR
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFG---IGQQTHR---------SLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRI 240
           MNLEVLKYCATVLFLESSLPEVF ENSWVSPNAVTRRR AKSSPQG++SNN+THSS SRI
Sbjct: 181 MNLEVLKYCATVLFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRI 240

Query: 241 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 300
           GG+PISL D QGETHPILSL+TCSSEDGASNLAES AT+S SFNM TLSDQITGESLETD
Sbjct: 241 GGNPISLLD-QGETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETD 300

Query: 301 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHK 360
           I+MEEE A+ STEASPSEDTSTSL+AT EFLEPDQVSVSFITASFVPFFRGSQRIQLSHK
Sbjct: 301 ISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHK 360

Query: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGS 420
           DDCLQLLCNNLRVRFGISTKFTDYAGRP+LSFVVDVPPSLC VLEASDGVAQRLFSDSGS
Sbjct: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGS 420

Query: 421 GSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAE 480
           GSEWRPVVMRKN YFNYPTMRLHIPTAVSGD AIYATEMYQKESSGAAQRLIFSKFDAAE
Sbjct: 421 GSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAE 480

Query: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LDSLIKPGAILDAFISLDTYDYQQSAGIRL
Sbjct: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 497

BLAST of Clc06G17220 vs. NCBI nr
Match: KAA0057957.1 (protein NEN1 [Cucumis melo var. makuwa])

HSP 1 Score: 864.4 bits (2232), Expect = 5.6e-247
Identity = 449/510 (88.04%), Postives = 469/510 (91.96%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           M   EDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           SSLSVRCNGITRDAVISSPTFA IADRVYDILHGRIWAGHNILRFDCARIREAFA+IG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHA                + SLDDVR
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHA-------------NSGDQSLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRI 240
           MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG++SNNN+H+S S I
Sbjct: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNI 240

Query: 241 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 300
           GG+PISLTDQQGE HPILSL+TCSSEDGA NLAE SAT+S SFNMHT+SDQITG +LETD
Sbjct: 241 GGNPISLTDQQGEAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETD 300

Query: 301 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHK 360
           INME E A+ STEASPSEDTSTSL+ T +FLEPDQVSV FITASFVPFFRGSQRIQL HK
Sbjct: 301 INMEGEHATVSTEASPSEDTSTSLHTT-KFLEPDQVSVLFITASFVPFFRGSQRIQLWHK 360

Query: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGS 420
           DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLC VLEASDGVAQRLFSDSGS
Sbjct: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGS 420

Query: 421 GSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAE 480
           GSEWRP V RKNGYFNYPTMRLHIPTAVSGD A YATEMYQK++SGA QRLIFSKFDAAE
Sbjct: 421 GSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAE 480

Query: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LDSLIKPGAILDAFISLDTYDYQQSAGIRL
Sbjct: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 496

BLAST of Clc06G17220 vs. NCBI nr
Match: XP_008453220.1 (PREDICTED: protein NEN1 [Cucumis melo])

HSP 1 Score: 847.4 bits (2188), Expect = 7.1e-242
Identity = 442/510 (86.67%), Postives = 463/510 (90.78%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           M   EDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           SSLSVRCNGITRDAVISSPTFA IADRVYDILHGRIWAGHNILRFDCARIREAFA+IG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEPKGTIDSLALLTQRFGRRAGDMK+ T  S      +    H          SLDDVR
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFG---IGQQTHR---------SLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRI 240
           MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG++SNNN+H+S S I
Sbjct: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNI 240

Query: 241 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 300
           GG+PISLTDQQGE HPILSL+TCSSEDGA NLAE SAT+S SFNMHT+SDQITG +LETD
Sbjct: 241 GGNPISLTDQQGEAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETD 300

Query: 301 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHK 360
           INME E A+ STEASPSEDTSTSL+ T +FLEPDQVSV FITASFVPFFRGSQRIQL HK
Sbjct: 301 INMEGEHATVSTEASPSEDTSTSLHTT-KFLEPDQVSVLFITASFVPFFRGSQRIQLWHK 360

Query: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGS 420
           DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLC VLEASDGVAQRLFSDSGS
Sbjct: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGS 420

Query: 421 GSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAE 480
           GSEWRP V RKNGYFNYPTMRLHIPTAVSGD A YATEMYQK++SGA QRLIFSKFDAAE
Sbjct: 421 GSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAE 480

Query: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LDSLIKPGAILDAFISLDTYDYQQSAGIRL
Sbjct: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 497

BLAST of Clc06G17220 vs. NCBI nr
Match: XP_022921575.1 (protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 832.4 bits (2149), Expect = 2.4e-237
Identity = 440/520 (84.62%), Postives = 459/520 (88.27%), Query Frame = 0

Query: 6   DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSV 65
           DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLISSLSV
Sbjct: 6   DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLISSLSV 65

Query: 66  RCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEPK 125
           RCNGITRDAVIS+PTFA IADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAP+PK
Sbjct: 66  RCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPK 125

Query: 126 GTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNLEV 185
           GTIDSLALLTQ+FGRRAGDMK+ T  S      +    H          SLDDVRMNLEV
Sbjct: 126 GTIDSLALLTQKFGRRAGDMKMATLASYFG---IGQQTHR---------SLDDVRMNLEV 185

Query: 186 LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNN-------------- 245
           LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG N+NN              
Sbjct: 186 LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANS 245

Query: 246 NTHSSRSRIGGHPISLTDQQGETHPILSLLTCSSEDGASNL-AESSATDSGSFNMHTLSD 305
           NT SS SRIGG PISLTDQQGE HPILSL+TCSSEDGASNL A+S AT+S SFNM TLSD
Sbjct: 246 NTRSSSSRIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSD 305

Query: 306 QITGESLETDINMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFR 365
           QI GESLETD +MEEE AS STEASP+E+TSTS+++  EFLEPDQVSVS ITASFVPFFR
Sbjct: 306 QIAGESLETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFR 365

Query: 366 GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGV 425
           GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPPSLC VLEASDGV
Sbjct: 366 GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGV 425

Query: 426 AQRLFSDSGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQR 485
           AQRLFS+SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGD AIYATEMYQKE+SG AQR
Sbjct: 426 AQRLFSESGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQR 485

Query: 486 LIFSKFDAAELDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LIFSKFDA ELDSLI PG I+DAFISLDTYDYQQSAGIRL
Sbjct: 486 LIFSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRL 513

BLAST of Clc06G17220 vs. NCBI nr
Match: XP_011660176.1 (protein NEN1 [Cucumis sativus] >KGN63687.1 hypothetical protein Csa_013241 [Cucumis sativus])

HSP 1 Score: 829.7 bits (2142), Expect = 1.5e-236
Identity = 439/510 (86.08%), Postives = 458/510 (89.80%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           M   EDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           SSLSVRCNGITRDAVISSPTFA IADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEPKGTIDSLALLTQRFGRRAGDMK+ T  S      +    H          SLDDVR
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFG---IGQQTHR---------SLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRI 240
           MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG++SNNN+H+S S I
Sbjct: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNI 240

Query: 241 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 300
           GG+PISL  QQGETHPILSL+T  SED AS LAE SAT+S SFNMHT SDQITG +LETD
Sbjct: 241 GGNPISLPVQQGETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETD 300

Query: 301 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHK 360
           INM EE A+ STEASPSEDTSTSL  T +FLEPDQVSVSFITASFVPFFRGSQRIQL HK
Sbjct: 301 INM-EEHATVSTEASPSEDTSTSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHK 360

Query: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGS 420
           DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPP+LC VLEASDGVAQRLFSDSGS
Sbjct: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGS 420

Query: 421 GSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAE 480
           GSEWRP V RKNGYFNYPTMRLHIPTAVSGD A YATEM+QKE+SGA QRLIFSKFDAAE
Sbjct: 421 GSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAE 480

Query: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LDSLIKPGAILDAFISLDTYDYQQSAGIRL
Sbjct: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 496

BLAST of Clc06G17220 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.9e-134
Identity = 280/509 (55.01%), Postives = 344/509 (67.58%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQRF+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEP 124
           VRCNGI RD V+ +P FADIAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNLE 184
           KG IDSL LLTQ+FGRRAGDMK+ T   L     +    H          SLDDVRMNLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMAT---LARYFGLGNQTHR---------SLDDVRMNLE 183

Query: 185 VLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRIGGHP 244
           VLKYCATVLFLESSLP    +NS VSP  ++ RR   +S +G     NT ++  R+    
Sbjct: 184 VLKYCATVLFLESSLPYAHVDNS-VSPETISSRRRIDASREG-----NTVTTSVRL---- 243

Query: 245 ISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGES-LETDINM 304
                                     +++E+SA     FNM  L +++  ++ +++DI M
Sbjct: 244 -------------------------PSISENSAAQPDPFNMSVLRNEMASDNHIQSDILM 303

Query: 305 EEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKD 364
           EEEQ   S +   SE+TS        FL PD +S+S I A   PF+ GSQ  +++L H D
Sbjct: 304 EEEQIEPS-DVVASENTSDH----EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGD 363

Query: 365 DCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGSG 424
             LQL C+ L++RFG++ KF D  GR RL+FVVD+ PSL  +LEA D  AQ+L  DSGS 
Sbjct: 364 SPLQLYCSYLKIRFGVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGST 423

Query: 425 SEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAEL 484
           SEW PVV    G+ NYP  R+HI T ++GD A YATE++Q+ESSGA Q+LIFS  +  EL
Sbjct: 424 SEWNPVVNPMKGFVNYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEEL 459

Query: 485 DSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           +SL+  G+++DAF+SL+ YDYQQ AGIRL
Sbjct: 484 ESLLTTGSVVDAFLSLEPYDYQQKAGIRL 459

BLAST of Clc06G17220 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 2.9e-129
Identity = 275/514 (53.50%), Postives = 340/514 (66.15%), Query Frame = 0

Query: 4   AEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSL 63
           AEDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+  
Sbjct: 10  AEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPR 69

Query: 64  SVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPE 123
           SV+CNGI R+ V S+ TFADIAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   PE
Sbjct: 70  SVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPE 129

Query: 124 PKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNL 183
           PKGTIDSLALLTQRFGRRAGDMK+ T      A Y              + SLDDVRMN 
Sbjct: 130 PKGTIDSLALLTQRFGRRAGDMKMAT-----LATYFGLGNQT-------HRSLDDVRMNF 189

Query: 184 EVLKYCATVLFLESSLPEVFPENSWVSPNAVT---RRRAAKSSPQGDNSNNNTHSSRSRI 243
           EVLKYCATVLFLESSLP+   ENS  +    T   RRR  K SP    ++  T  + + I
Sbjct: 190 EVLKYCATVLFLESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQSPTDQQTGENMTTI 249

Query: 244 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 303
                          PILS ++            S+   +  F+M TL ++I  E L++D
Sbjct: 250 ---------------PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSD 309

Query: 304 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQ--RIQLS 363
           + MEEEQ +  +E   SE T         F+E D++SVS I A+ VP + GSQ  ++QL 
Sbjct: 310 VPMEEEQ-NQQSETVASEGTGDQ----EGFMELDKISVSSIRATHVPLYDGSQTMKLQLF 369

Query: 364 HKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDS 423
             D  LQL C  L+VRFGI+ KF D AGR RL+FV+D+ PSLC VL+  D  AQ +  DS
Sbjct: 370 LGDRPLQLHCPRLKVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDS 429

Query: 424 GSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSG--AAQRLIFSKF 483
           GSGS+W P+V+   G+ N PT R+HIPT ++GD   YA E++QKE SG  A Q+LI S  
Sbjct: 430 GSGSDWNPLVIPMKGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNP 479

Query: 484 DAAELDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
            A E++SL+ P  +LDAF+SL+ YDYQQ AGIRL
Sbjct: 490 KAEEIESLLNPRTVLDAFLSLEPYDYQQRAGIRL 479

BLAST of Clc06G17220 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.4e-128
Identity = 260/508 (51.18%), Postives = 339/508 (66.73%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNLE 184
           K TIDSL+LL+Q+FG+RAGDMK+ +      A Y      A       + SLDDVRMNLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMAS-----LATYFGLGDQA-------HRSLDDVRMNLE 187

Query: 185 VLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRA-AKSSPQGDNSNNNTHSSRSRIGGH 244
           V+KYCATVLFLESS+P++  + SW SP    R R+  K    G   ++ + SS  +    
Sbjct: 188 VVKYCATVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPS 247

Query: 245 PISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETDINM 304
             S+     + HPI+SLLT  SE  AS+       D     + TL  ++   +L+TD   
Sbjct: 248 SSSVDATIVKNHPIVSLLTECSESDASSYDIEDPID-----ITTLIGKLRIGTLQTDAAE 307

Query: 305 EEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDC 364
           E +      E+ PS D+         FL  ++VSVS I AS VPF+RGS R++L H D  
Sbjct: 308 EAKTVRQQDESPPSPDSDAK---DESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTP 367

Query: 365 LQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGSGSE 424
           L L  ++L+VRFGIS KF D+AGRP+L+ +VD P  LC +L+A D  A  L +DS + S+
Sbjct: 368 LHLCWHSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSD 427

Query: 425 WRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESS-GAAQRLIFSKFDAAELD 484
           WRP V+RK G+ NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +L+
Sbjct: 428 WRPTVIRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLE 487

Query: 485 SLIKPGAILDAFISLDTYDYQQSAGIRL 511
           S + PG ++DAF SL+ Y YQQ AGIRL
Sbjct: 488 SALLPGTLVDAFFSLEPYSYQQMAGIRL 495

BLAST of Clc06G17220 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.1e-59
Identity = 120/218 (55.05%), Postives = 155/218 (71.10%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MA+    +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++
Sbjct: 1   MAVQTFPNEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVV 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           S  S R +GITR  V ++P+F D+A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  
Sbjct: 61  SIRSSRSDGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKA 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEP G IDSL LL+ +FG+RAG+MK+ +      A Y    V        ++ SLDDVR
Sbjct: 121 APEPSGIIDSLGLLSDKFGKRAGNMKMAS-----LAAYFGLGVQ-------KHRSLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRR 219
           MNLEVLK+CATVLFLES+LP         S   +TR R
Sbjct: 181 MNLEVLKHCATVLFLESTLPNHLEGKWHTSSKIMTRSR 206

BLAST of Clc06G17220 vs. ExPASy TrEMBL
Match: A0A5A7UTA8 (Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 PE=4 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 2.7e-247
Identity = 449/510 (88.04%), Postives = 469/510 (91.96%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           M   EDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           SSLSVRCNGITRDAVISSPTFA IADRVYDILHGRIWAGHNILRFDCARIREAFA+IG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHA                + SLDDVR
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHA-------------NSGDQSLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRI 240
           MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG++SNNN+H+S S I
Sbjct: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNI 240

Query: 241 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 300
           GG+PISLTDQQGE HPILSL+TCSSEDGA NLAE SAT+S SFNMHT+SDQITG +LETD
Sbjct: 241 GGNPISLTDQQGEAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETD 300

Query: 301 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHK 360
           INME E A+ STEASPSEDTSTSL+ T +FLEPDQVSV FITASFVPFFRGSQRIQL HK
Sbjct: 301 INMEGEHATVSTEASPSEDTSTSLHTT-KFLEPDQVSVLFITASFVPFFRGSQRIQLWHK 360

Query: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGS 420
           DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLC VLEASDGVAQRLFSDSGS
Sbjct: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGS 420

Query: 421 GSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAE 480
           GSEWRP V RKNGYFNYPTMRLHIPTAVSGD A YATEMYQK++SGA QRLIFSKFDAAE
Sbjct: 421 GSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAE 480

Query: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LDSLIKPGAILDAFISLDTYDYQQSAGIRL
Sbjct: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 496

BLAST of Clc06G17220 vs. ExPASy TrEMBL
Match: A0A1S3BWV4 (protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.4e-242
Identity = 442/510 (86.67%), Postives = 463/510 (90.78%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           M   EDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           SSLSVRCNGITRDAVISSPTFA IADRVYDILHGRIWAGHNILRFDCARIREAFA+IG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEPKGTIDSLALLTQRFGRRAGDMK+ T  S      +    H          SLDDVR
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFG---IGQQTHR---------SLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRI 240
           MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG++SNNN+H+S S I
Sbjct: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNI 240

Query: 241 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 300
           GG+PISLTDQQGE HPILSL+TCSSEDGA NLAE SAT+S SFNMHT+SDQITG +LETD
Sbjct: 241 GGNPISLTDQQGEAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETD 300

Query: 301 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHK 360
           INME E A+ STEASPSEDTSTSL+ T +FLEPDQVSV FITASFVPFFRGSQRIQL HK
Sbjct: 301 INMEGEHATVSTEASPSEDTSTSLHTT-KFLEPDQVSVLFITASFVPFFRGSQRIQLWHK 360

Query: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGS 420
           DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLC VLEASDGVAQRLFSDSGS
Sbjct: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGS 420

Query: 421 GSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAE 480
           GSEWRP V RKNGYFNYPTMRLHIPTAVSGD A YATEMYQK++SGA QRLIFSKFDAAE
Sbjct: 421 GSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAE 480

Query: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LDSLIKPGAILDAFISLDTYDYQQSAGIRL
Sbjct: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 497

BLAST of Clc06G17220 vs. ExPASy TrEMBL
Match: A0A6J1E665 (protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.1e-237
Identity = 440/520 (84.62%), Postives = 459/520 (88.27%), Query Frame = 0

Query: 6   DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSV 65
           DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLISSLSV
Sbjct: 6   DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLISSLSV 65

Query: 66  RCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEPK 125
           RCNGITRDAVIS+PTFA IADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAP+PK
Sbjct: 66  RCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPK 125

Query: 126 GTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNLEV 185
           GTIDSLALLTQ+FGRRAGDMK+ T  S      +    H          SLDDVRMNLEV
Sbjct: 126 GTIDSLALLTQKFGRRAGDMKMATLASYFG---IGQQTHR---------SLDDVRMNLEV 185

Query: 186 LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNN-------------- 245
           LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG N+NN              
Sbjct: 186 LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANS 245

Query: 246 NTHSSRSRIGGHPISLTDQQGETHPILSLLTCSSEDGASNL-AESSATDSGSFNMHTLSD 305
           NT SS SRIGG PISLTDQQGE HPILSL+TCSSEDGASNL A+S AT+S SFNM TLSD
Sbjct: 246 NTRSSSSRIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSD 305

Query: 306 QITGESLETDINMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFR 365
           QI GESLETD +MEEE AS STEASP+E+TSTS+++  EFLEPDQVSVS ITASFVPFFR
Sbjct: 306 QIAGESLETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFR 365

Query: 366 GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGV 425
           GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPPSLC VLEASDGV
Sbjct: 366 GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGV 425

Query: 426 AQRLFSDSGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQR 485
           AQRLFS+SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGD AIYATEMYQKE+SG AQR
Sbjct: 426 AQRLFSESGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQR 485

Query: 486 LIFSKFDAAELDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LIFSKFDA ELDSLI PG I+DAFISLDTYDYQQSAGIRL
Sbjct: 486 LIFSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRL 513

BLAST of Clc06G17220 vs. ExPASy TrEMBL
Match: A0A0A0LSC3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910 PE=4 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 7.4e-237
Identity = 439/510 (86.08%), Postives = 458/510 (89.80%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           M   EDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           SSLSVRCNGITRDAVISSPTFA IADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEPKGTIDSLALLTQRFGRRAGDMK+ T  S      +    H          SLDDVR
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFG---IGQQTHR---------SLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRI 240
           MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG++SNNN+H+S S I
Sbjct: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNI 240

Query: 241 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 300
           GG+PISL  QQGETHPILSL+T  SED AS LAE SAT+S SFNMHT SDQITG +LETD
Sbjct: 241 GGNPISLPVQQGETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETD 300

Query: 301 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHK 360
           INM EE A+ STEASPSEDTSTSL  T +FLEPDQVSVSFITASFVPFFRGSQRIQL HK
Sbjct: 301 INM-EEHATVSTEASPSEDTSTSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHK 360

Query: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGS 420
           DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPP+LC VLEASDGVAQRLFSDSGS
Sbjct: 361 DDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGS 420

Query: 421 GSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAE 480
           GSEWRP V RKNGYFNYPTMRLHIPTAVSGD A YATEM+QKE+SGA QRLIFSKFDAAE
Sbjct: 421 GSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAE 480

Query: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LDSLIKPGAILDAFISLDTYDYQQSAGIRL
Sbjct: 481 LDSLIKPGAILDAFISLDTYDYQQSAGIRL 496

BLAST of Clc06G17220 vs. ExPASy TrEMBL
Match: A0A6J1JJE4 (protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 9.7e-237
Identity = 440/520 (84.62%), Postives = 456/520 (87.69%), Query Frame = 0

Query: 6   DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSV 65
           DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLISSLSV
Sbjct: 6   DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLISSLSV 65

Query: 66  RCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEPK 125
           RCNGITRDAVIS+PTFA IADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAP+PK
Sbjct: 66  RCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPK 125

Query: 126 GTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNLEV 185
           GTIDSLALLTQ+FGRRAGDMK+ T  S      +    H          SLDDVRMNLEV
Sbjct: 126 GTIDSLALLTQKFGRRAGDMKMATLASYFG---IGQQTHR---------SLDDVRMNLEV 185

Query: 186 LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNN-------------- 245
           LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG N NN              
Sbjct: 186 LKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANS 245

Query: 246 NTHSSRSRIGGHPISLTDQQGETHPILSLLTCSSEDGASNL-AESSATDSGSFNMHTLSD 305
           NT SS SRIGG PISLTDQQGE HPILSL+TCSSEDGASNL A+S AT+S SFNM TLSD
Sbjct: 246 NTRSSSSRIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSD 305

Query: 306 QITGESLETDINMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFR 365
           QI GESLETD +MEEE A  STEASP+E+TSTSL+A  EFLEPDQVSVS ITASFVPFFR
Sbjct: 306 QIAGESLETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFR 365

Query: 366 GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGV 425
           GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPPSLC VLEASDGV
Sbjct: 366 GSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGV 425

Query: 426 AQRLFSDSGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQR 485
           AQRLFS+SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAV GD AIYATEMYQKE+SG AQR
Sbjct: 426 AQRLFSESGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQR 485

Query: 486 LIFSKFDAAELDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           LIFSKFDA ELDSLI PG I+DAFISLDTYDYQQSAGIRL
Sbjct: 486 LIFSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRL 513

BLAST of Clc06G17220 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.8e-135
Identity = 280/509 (55.01%), Postives = 344/509 (67.58%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQRF+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEP 124
           VRCNGI RD V+ +P FADIAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNLE 184
           KG IDSL LLTQ+FGRRAGDMK+ T   L     +    H          SLDDVRMNLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMAT---LARYFGLGNQTHR---------SLDDVRMNLE 183

Query: 185 VLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGDNSNNNTHSSRSRIGGHP 244
           VLKYCATVLFLESSLP    +NS VSP  ++ RR   +S +G     NT ++  R+    
Sbjct: 184 VLKYCATVLFLESSLPYAHVDNS-VSPETISSRRRIDASREG-----NTVTTSVRL---- 243

Query: 245 ISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGES-LETDINM 304
                                     +++E+SA     FNM  L +++  ++ +++DI M
Sbjct: 244 -------------------------PSISENSAAQPDPFNMSVLRNEMASDNHIQSDILM 303

Query: 305 EEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKD 364
           EEEQ   S +   SE+TS        FL PD +S+S I A   PF+ GSQ  +++L H D
Sbjct: 304 EEEQIEPS-DVVASENTSDH----EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGD 363

Query: 365 DCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGSG 424
             LQL C+ L++RFG++ KF D  GR RL+FVVD+ PSL  +LEA D  AQ+L  DSGS 
Sbjct: 364 SPLQLYCSYLKIRFGVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGST 423

Query: 425 SEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAEL 484
           SEW PVV    G+ NYP  R+HI T ++GD A YATE++Q+ESSGA Q+LIFS  +  EL
Sbjct: 424 SEWNPVVNPMKGFVNYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEEL 459

Query: 485 DSLIKPGAILDAFISLDTYDYQQSAGIRL 511
           +SL+  G+++DAF+SL+ YDYQQ AGIRL
Sbjct: 484 ESLLTTGSVVDAFLSLEPYDYQQKAGIRL 459

BLAST of Clc06G17220 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 463.8 bits (1192), Expect = 2.1e-130
Identity = 275/514 (53.50%), Postives = 340/514 (66.15%), Query Frame = 0

Query: 4   AEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSL 63
           AEDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+  
Sbjct: 10  AEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPR 69

Query: 64  SVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPE 123
           SV+CNGI R+ V S+ TFADIAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   PE
Sbjct: 70  SVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPE 129

Query: 124 PKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNL 183
           PKGTIDSLALLTQRFGRRAGDMK+ T      A Y              + SLDDVRMN 
Sbjct: 130 PKGTIDSLALLTQRFGRRAGDMKMAT-----LATYFGLGNQT-------HRSLDDVRMNF 189

Query: 184 EVLKYCATVLFLESSLPEVFPENSWVSPNAVT---RRRAAKSSPQGDNSNNNTHSSRSRI 243
           EVLKYCATVLFLESSLP+   ENS  +    T   RRR  K SP    ++  T  + + I
Sbjct: 190 EVLKYCATVLFLESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQSPTDQQTGENMTTI 249

Query: 244 GGHPISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETD 303
                          PILS ++            S+   +  F+M TL ++I  E L++D
Sbjct: 250 ---------------PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSD 309

Query: 304 INMEEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQ--RIQLS 363
           + MEEEQ +  +E   SE T         F+E D++SVS I A+ VP + GSQ  ++QL 
Sbjct: 310 VPMEEEQ-NQQSETVASEGTGDQ----EGFMELDKISVSSIRATHVPLYDGSQTMKLQLF 369

Query: 364 HKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDS 423
             D  LQL C  L+VRFGI+ KF D AGR RL+FV+D+ PSLC VL+  D  AQ +  DS
Sbjct: 370 LGDRPLQLHCPRLKVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDS 429

Query: 424 GSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSG--AAQRLIFSKF 483
           GSGS+W P+V+   G+ N PT R+HIPT ++GD   YA E++QKE SG  A Q+LI S  
Sbjct: 430 GSGSDWNPLVIPMKGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNP 479

Query: 484 DAAELDSLIKPGAILDAFISLDTYDYQQSAGIRL 511
            A E++SL+ P  +LDAF+SL+ YDYQQ AGIRL
Sbjct: 490 KAEEIESLLNPRTVLDAFLSLEPYDYQQRAGIRL 479

BLAST of Clc06G17220 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 461.5 bits (1186), Expect = 1.0e-129
Identity = 260/508 (51.18%), Postives = 339/508 (66.73%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNLE 184
           K TIDSL+LL+Q+FG+RAGDMK+ +      A Y      A       + SLDDVRMNLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMAS-----LATYFGLGDQA-------HRSLDDVRMNLE 187

Query: 185 VLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRA-AKSSPQGDNSNNNTHSSRSRIGGH 244
           V+KYCATVLFLESS+P++  + SW SP    R R+  K    G   ++ + SS  +    
Sbjct: 188 VVKYCATVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPS 247

Query: 245 PISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETDINM 304
             S+     + HPI+SLLT  SE  AS+       D     + TL  ++   +L+TD   
Sbjct: 248 SSSVDATIVKNHPIVSLLTECSESDASSYDIEDPID-----ITTLIGKLRIGTLQTDAAE 307

Query: 305 EEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDC 364
           E +      E+ PS D+         FL  ++VSVS I AS VPF+RGS R++L H D  
Sbjct: 308 EAKTVRQQDESPPSPDSDAK---DESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTP 367

Query: 365 LQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGSGSE 424
           L L  ++L+VRFGIS KF D+AGRP+L+ +VD P  LC +L+A D  A  L +DS + S+
Sbjct: 368 LHLCWHSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSD 427

Query: 425 WRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESS-GAAQRLIFSKFDAAELD 484
           WRP V+RK G+ NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +L+
Sbjct: 428 WRPTVIRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLE 487

Query: 485 SLIKPGAILDAFISLDTYDYQQSAGIRL 511
           S + PG ++DAF SL+ Y YQQ AGIRL
Sbjct: 488 SALLPGTLVDAFFSLEPYSYQQMAGIRL 495

BLAST of Clc06G17220 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 433.0 bits (1112), Expect = 3.9e-121
Identity = 250/508 (49.21%), Postives = 328/508 (64.57%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVRMNLE 184
           K TIDSL+LL+Q+FG+RAGDMK+ +      A Y      A       + SLDDVRMNLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMAS-----LATYFGLGDQA-------HRSLDDVRMNLE 187

Query: 185 VLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRA-AKSSPQGDNSNNNTHSSRSRIGGH 244
                      ESS+P++  + SW SP    R R+  K    G   ++ + SS  +    
Sbjct: 188 -----------ESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPS 247

Query: 245 PISLTDQQGETHPILSLLTCSSEDGASNLAESSATDSGSFNMHTLSDQITGESLETDINM 304
             S+     + HPI+SLLT  SE  AS+       D     + TL  ++   +L+TD   
Sbjct: 248 SSSVDATIVKNHPIVSLLTECSESDASSYDIEDPID-----ITTLIGKLRIGTLQTDAAE 307

Query: 305 EEEQASSSTEASPSEDTSTSLNATPEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDC 364
           E +      E+ PS D+         FL  ++VSVS I AS VPF+RGS R++L H D  
Sbjct: 308 EAKTVRQQDESPPSPDSDAK---DESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTP 367

Query: 365 LQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPSLCMVLEASDGVAQRLFSDSGSGSE 424
           L L  ++L+VRFGIS KF D+AGRP+L+ +VD P  LC +L+A D  A  L +DS + S+
Sbjct: 368 LHLCWHSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSD 427

Query: 425 WRPVVMRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESS-GAAQRLIFSKFDAAELD 484
           WRP V+RK G+ NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +L+
Sbjct: 428 WRPTVIRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLE 484

Query: 485 SLIKPGAILDAFISLDTYDYQQSAGIRL 511
           S + PG ++DAF SL+ Y YQQ AGIRL
Sbjct: 488 SALLPGTLVDAFFSLEPYSYQQMAGIRL 484

BLAST of Clc06G17220 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 7.7e-61
Identity = 120/218 (55.05%), Postives = 155/218 (71.10%), Query Frame = 0

Query: 1   MALAEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MA+    +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++
Sbjct: 1   MAVQTFPNEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVV 60

Query: 61  SSLSVRCNGITRDAVISSPTFADIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120
           S  S R +GITR  V ++P+F D+A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  
Sbjct: 61  SIRSSRSDGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKA 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHACYVSACVHAKIQVILRNLSLDDVR 180
           APEP G IDSL LL+ +FG+RAG+MK+ +      A Y    V        ++ SLDDVR
Sbjct: 121 APEPSGIIDSLGLLSDKFGKRAGNMKMAS-----LAAYFGLGVQ-------KHRSLDDVR 180

Query: 181 MNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRR 219
           MNLEVLK+CATVLFLES+LP         S   +TR R
Sbjct: 181 MNLEVLKHCATVLFLESTLPNHLEGKWHTSSKIMTRSR 206