Clc06G15110 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc06G15110
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	isoamylase 1, chloroplastic
Location	ClcChr06: 26046555 .. 26051871 (+)
RNA-Seq Expression	Clc06G15110
Synteny	Clc06G15110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

atgagtagttttgctcccgtgaacaaagaggctctctgcagtgccttcaacaatgtggaaatcggggctaaggaggtagggtggggatggaaaatgttaaggacagtttcttgccggatcctgttaggtttggtgctatgaaaaattgggccctttctttagtggtctcccatttttgtgggcttgttttttggttgtccatgtatttttttcattttattctcaatgaaaggtatatctaggacatttttcaaggtttgagatgtggcaaaaggtttaatgtctcccacagttggtcagtaggttcttttgtaactatgattatagtttgtgtttgttggattcaagtcactttccatagtttatcagggtttccgtttttctttctttccttctttttcttttggctgggctgatttttttttatgccatttttattagaagctcagtttcttaaaaagaaatcgtgaaaatttatggatttccttccatgcattctattttattgtagagtgtcatgcatgtgatataaatgaaatatgccctcctcgaagacatgatttaaagtatttagtcttctttctgatctcatggtaggtattttgtattgattgaaatagatgctcaccatctcatcttaatagtagcgtgtaaaccgaacatgtagctccatggtggagaacaagtcactgtgatcgattctatttgctccctttttttttggttatcattatggattagtcttattccttttccttttattaatagctcattgcagaagcatgggatgctgggggcttataccaagttggcacattccctcactggggtgtttggtcagaatggaacgggaaggtctgcatagatatttgccattttcttctcattcccttatatttgatcgaaatattgagaggtctaatctaccaaattgaaagttaaaagtaaatgattgccacattgcctaaacacaaaataataaaaataatgactggaatttggaggatcgtcagttaaccaagtaacttttagatttctcaggagtcttaatgactctcaatgtgctttgagaacttctagttatgttccagcatattttggtactgtttatgatatttgaccagaacttttcttttttggatcaatttcagtatcgagacattgtacggcagttcatcaaaggcacagatgggttttcaggagcttttgctgaatgcctttgtgggagccctaatctttaccaggttacgtagagtttctcttccctgtttttagtgaaataaggttacttttcttcagatggtttaattaatccggctctgtcaacattttattggaaggataacagaaatagcataatagctttggatatattttctagaatgaagaaaatagagttgggatttggaagtaataaacaaagaaccattgcacactcatagtggcatcataacggaggtttactttggcacaagacttcctgtggagcatgaatattagaaatcagcataatggttacattgatagggaatagcggtacgagtagaaggtctgtgagttgtgttttgcccatgataagtgattctgcctgctttaagtggatggaaatattacaaattgaattttcatttttactgtattgaaccatatcatatcataatatttttttagtttttctaattcttctagttcttttatatagcatattaaataatgctgatgatcacaagtatgccacaattattttttcatatgattattaaaatttaaaatgattatttttttggagaaaaaatttatggagtgagggaaacaattgcatataaagcaacacaacactctcttttgatagatttgtacactgtctttctacacccgctaaaatggtgcaaattttatgcccctttaccaatttccgtttggttgatcttttggctaattggaaggcgtttatgaactttctttaggtccgggttctttccatccttttatatatttcatgacatcaatgaattatcatctaattaccctggtcaaagaaacaagtatattttatttgaagtctaaactttggttatgtcttttgtttgtcccattagttatagtgttggataggatattttcatattgagtagatttctaaccatgttttttaatttgcatattgagttgaatatctaacaacgtttttacagggaggaggaaggaaaccatggaatagtatcaactttatttgtgcacatgatggtttcagtttggctgatttagtgacttacaatagtaaacataacttagcaaatggtgaagacaacaatgacggtgaaaatcataataatagctggaactgtggacaggtatacaatcagttgtaggcatctcataagatcattgtcgatgtggtatgtaagataaaatcattggaccttaataacagtaggcaaaccttaggattaggtattgtttatcaaacagaccaaaacctctttaatgccttcttctggttaaagctcaattccacttatggcttcagaatcctgttggactcctctgcaacagatttttcctaaaaagatattcgtatgaaattttcatgagatagtaagaattgttgtctgaaagtctataccatttagtgttcaatgggttgaagtaactcacggatagggattgggttatgttttaatctatttcatcaatcaatgcaatggtttatgttttctatttaagaaataacttcgaagtattaaaggcttctactttttatttgttaggatgagtgttaatctttctatacgggtgcctccagagagcttcttataccttgttggagcctgtcatataaactcgatatgttttttgggttctgtttattatcattatttttgcgttattttctccctttttgatgtgcccgtttctcatattatacaggagggagagtttgttagcatctcagtaaagaaattgaggaaaagacagatgcggaatttctttgtctgcctcatggtttcccaagtaaggttcactgatattgtatatgacctgacaattgtttataatgattaaactaatgcacaaagaatttctcgctcatatgtcatatcatactggacgtgtcaatcagattatagggacatcatattttctttcatagacttgtacaagcattgttctctgggttagacatagctctagtcatgtgccagttattcggtcataagctagaatagttattgaaagtttagctatgcatgtatgcgaaattgtgacatttacggcaataatatttatgtccctcaaaggcaatcgtttttggcttactttccaattcttcaataaatcaaggttgcatttgaatcccgttcatgtaggaaattctgatgataccttaatagaaaattaacatgtttcttttaaaaaattgttcatagtgtattttttcatttttcttctgaatgaaaggtatatatatatgttcttaggaatctcatatgaatgagtaggaacatcccaatatcacccttgacttgagaaaaaatagaatcaaataaatttttatgaatattggcatttgaaattaacataaattaatttaatatattgaactactacaaactaatttattttccttttagtaagacagaaaaaacagcgatttgttagataaaaggcctattagatgcataatatcttaagatagccgacattctgaactgtaggcaaaaagattatgagaatttggaaatctgaaggtgggtggtatccaaagattccaataaaatgaagacaacgatttggcaacttgacttcacgtcttcactactaaacattttaatctacggtgaaaattaacatgtgggttgtttgtgcttcaatagtaaaattacttcaattctgaaccagattcttcctcctcgatgtattctaactttaaatcctgatttaattgattgggttactgctctcttctccgaactcacattcttgtaatatggttcgattacatgcacattgcatatataactctggccacagtgttggtatattactccagtggcaacatttatttcatcatagttgctccctactgtaataataagtctaaaaacatgtacgatagaactttgtccaatttgtcgataaaattttttaaaaaattagttttttgaaatttgttctgactacttttatccgatcagggcgtcccaatgatattaatgggtgatgaatacggtcacacaaagggggggaacaacaatacgtattgccacgataactatgtagttcctttatcttgctttattctgaacttttttttgttgctcaaaattcaattctgttgacctgagctactaactttgctacagctcaattatttccgttgggataagttggaagagtcatcctccgacttccacagattttgcagtcttatgaccacattccgttagtaagttccttctgcattgatttgtttcgggagttcagtgttgtaaatgtctaataatcctatctttctatgctcttgcagcgaatgtgagtcacttggcttaaatgatttcccaacagatgagagattgcaatggcatggtcattctcctggaaagccagactggactgaagcgagccgtttcgtggccttcacattggtacattcccttttccaattaatatttgctcacacaacacaaaccatcctccttttctctactctggcatgttggcaaaattatcttcattcttcatatttttgctctgctgtcactaacaccagtacttaacacacatcacatgttgcttttcaacttgctgagatcatgtcagttgttttatatactctaagattgattcttattttgagatggcttatttcccaggttgattcagtaaaacgagaagtttacattgccttcaatgccagtcatttacctgtcactgtgatgctgcccgagagacccggatacagatgggacccgttggtcgacacaggtaagatctcgccatttgacttcctcacaagtgaccttcctgaaagagatgtggcaatccaacagtatgctcatttccttgacgccaatctttatcccatgcttagttattcttccatcatccttctactctctccagtagaacctgcatcatcttgatcatcttccaattatgatatcccatttcccttaaataaaactgcttcctctggtgtgtgtgaatattttatatttacatgcactgtgggatgtccgtgacattatttctttatatcttaaattacttttttttactttttttttttgaaggcatatacgaagacacatag

mRNA sequence

atgagtagttttgctcccgtgaacaaagaggctctctgcagtgccttcaacaatgtggaaatcggggctaaggagctcattgcagaagcatgggatgctgggggcttataccaagttggcacattccctcactggggtgtttggtcagaatggaacgggaagtatcgagacattgtacggcagttcatcaaaggcacagatgggttttcaggagcttttgctgaatgcctttgtgggagccctaatctttaccagggaggaggaaggaaaccatggaatagtatcaactttatttgtgcacatgatggtttcagtttggctgatttagtgacttacaatagtaaacataacttagcaaatggtgaagacaacaatgacggtgaaaatcataataatagctggaactgtggacaggagggagagtttgttagcatctcagtaaagaaattgaggaaaagacagatgcggaatttctttgtctgcctcatggtttcccaaggcgtcccaatgatattaatgggtgatgaatacggtcacacaaagggggggaacaacaatacgtattgccacgataactatctcaattatttccgttgggataagttggaagagtcatcctccgacttccacagattttgcagtcttatgaccacattccgttacgaatgtgagtcacttggcttaaatgatttcccaacagatgagagattgcaatggcatggtcattctcctggaaagccagactggactgaagcgagccgtttcgtggccttcacattggttgattcagtaaaacgagaagtttacattgccttcaatgccagtcatttacctgtcactgtgatgctgcccgagagacccggatacagatgggacccgttggtcgacacaggcatatacgaagacacatag

Coding sequence (CDS)

atgagtagttttgctcccgtgaacaaagaggctctctgcagtgccttcaacaatgtggaaatcggggctaaggagctcattgcagaagcatgggatgctgggggcttataccaagttggcacattccctcactggggtgtttggtcagaatggaacgggaagtatcgagacattgtacggcagttcatcaaaggcacagatgggttttcaggagcttttgctgaatgcctttgtgggagccctaatctttaccagggaggaggaaggaaaccatggaatagtatcaactttatttgtgcacatgatggtttcagtttggctgatttagtgacttacaatagtaaacataacttagcaaatggtgaagacaacaatgacggtgaaaatcataataatagctggaactgtggacaggagggagagtttgttagcatctcagtaaagaaattgaggaaaagacagatgcggaatttctttgtctgcctcatggtttcccaaggcgtcccaatgatattaatgggtgatgaatacggtcacacaaagggggggaacaacaatacgtattgccacgataactatctcaattatttccgttgggataagttggaagagtcatcctccgacttccacagattttgcagtcttatgaccacattccgttacgaatgtgagtcacttggcttaaatgatttcccaacagatgagagattgcaatggcatggtcattctcctggaaagccagactggactgaagcgagccgtttcgtggccttcacattggttgattcagtaaaacgagaagtttacattgccttcaatgccagtcatttacctgtcactgtgatgctgcccgagagacccggatacagatgggacccgttggtcgacacaggcatatacgaagacacatag

Protein sequence

MSSFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTGIYEDT

Homology

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BLAST of Clc06G15110 vs. NCBI nr
Match: XP_038879251.1 (isoamylase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 590.9 bits (1522), Expect = 6.3e-165
Identity = 269/282 (95.39%), Postives = 276/282 (97.87%), Query Frame = 0


Query: 17  NNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 76
           N+  +   +LIAEAWDAGGLYQVGTFPHWGVWSEWNG+YRDIVRQFIKGTDGFSGAFAEC
Sbjct: 480 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAEC 539

Query: 77  LCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNC 136
           LCGSPNLYQGGGRKPWNSINFICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 540 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 599

Query: 137 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 196
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVP+ILMGDEYGHTKGGNNNTYCHDNYLNY
Sbjct: 600 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPLILMGDEYGHTKGGNNNTYCHDNYLNY 659

Query: 197 FRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 256
           FRWDK+EESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV
Sbjct: 660 FRWDKMEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 719

Query: 257 AFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           AFTLVDSVKREVYIAFNASHLPVTV LPERPGYRWDPLVDTG
Sbjct: 720 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTG 761

[Close]

BLAST of Clc06G15110 vs. NCBI nr
Match: TYK28533.1 (isoamylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 584.7 bits (1506), Expect = 4.5e-163
Identity = 266/282 (94.33%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 17  NNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 76
           N+  +   +LIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 156 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 215

Query: 77  LCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNC 136
           LCGSPNLYQGGGRKP NSINF+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 216 LCGSPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 275

Query: 137 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 196
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY
Sbjct: 276 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 335

Query: 197 FRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 256
           FRWDK+EES+SDFHRFCSLMTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV
Sbjct: 336 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 395

Query: 257 AFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           AFTLVDSVKREVYIAFNASHLPVTV LPERPGYRWDPLVDTG
Sbjct: 396 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTG 437

[Close]

BLAST of Clc06G15110 vs. NCBI nr
Match: XP_008453530.1 (PREDICTED: isoamylase 1, chloroplastic [Cucumis melo] >KAA0058172.1 isoamylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 584.7 bits (1506), Expect = 4.5e-163
Identity = 266/282 (94.33%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 17  NNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 76
           N+  +   +LIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 477 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 536

Query: 77  LCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNC 136
           LCGSPNLYQGGGRKP NSINF+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 537 LCGSPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 596

Query: 137 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 196
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY
Sbjct: 597 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 656

Query: 197 FRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 256
           FRWDK+EES+SDFHRFCSLMTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV
Sbjct: 657 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 716

Query: 257 AFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           AFTLVDSVKREVYIAFNASHLPVTV LPERPGYRWDPLVDTG
Sbjct: 717 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTG 758

[Close]

BLAST of Clc06G15110 vs. NCBI nr
Match: XP_004149676.1 (isoamylase 1, chloroplastic [Cucumis sativus] >KAE8653176.1 hypothetical protein Csa_019536 [Cucumis sativus])

HSP 1 Score: 580.5 bits (1495), Expect = 8.5e-162
Identity = 265/283 (93.64%), Postives = 275/283 (97.17%), Query Frame = 0


Query: 17  NNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 76
           N+  +   +LIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRD++RQFIKGTDGFSGAFAEC
Sbjct: 477 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVIRQFIKGTDGFSGAFAEC 536

Query: 77  LCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNC 136
           LCGSPNLYQGGGRKPWNSINFICAHDGF+LADLVTYNSK NLANGE+NNDGENHNNSWNC
Sbjct: 537 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKQNLANGEENNDGENHNNSWNC 596

Query: 137 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 196
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY
Sbjct: 597 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 656

Query: 197 FRWDKLEESSS-DFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRF 256
           FRWDK+EES+S DFHRFCSLMTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRF
Sbjct: 657 FRWDKMEESTSFDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRF 716

Query: 257 VAFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           VAFTLVDSVKREVYIAFNASHLPVTV LPERPGYRWDPLVDTG
Sbjct: 717 VAFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTG 759

[Close]

BLAST of Clc06G15110 vs. NCBI nr
Match: KAG7021674.1 (Isoamylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 574.3 bits (1479), Expect = 6.1e-160
Identity = 265/300 (88.33%), Postives = 279/300 (93.00%), Query Frame = 0


Query: 1   MSSFAPVNKEALCSAFNNVEI--GAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDI 60
           +++ +P+    L    +N  I  G K LIAEAWDAGGLYQVGTFPHW VWSEWNGKYRDI
Sbjct: 460 LTTGSPLGNPPLIDMISNDPILQGVK-LIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDI 519

Query: 61  VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNL 120
           VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGF+LADLVTYNSKHNL
Sbjct: 520 VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNL 579

Query: 121 ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYG 180
           ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYG
Sbjct: 580 ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYG 639

Query: 181 HTKGGNNNTYCHDNYLNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQ 240
           HTKGGNNNTYCHDNY+NYFRWDK+EESSSDFHRFC LMTTFRYECESLGLNDFPT +RLQ
Sbjct: 640 HTKGGNNNTYCHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQ 699

Query: 241 WHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           WHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHLPVTV LPERPGY+W+PLVDTG
Sbjct: 700 WHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTG 758

[Close]

BLAST of Clc06G15110 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 4.3e-140
Identity = 223/282 (79.08%), Postives = 246/282 (87.23%), Query Frame = 0


Query: 17  NNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 76
           N+  +G  +LIAEAWDAGGLYQVG FPHW +WSEWNGKYRDIVRQFIKGTDGF+G FAEC
Sbjct: 476 NDPILGDVKLIAEAWDAGGLYQVGQFPHWKIWSEWNGKYRDIVRQFIKGTDGFAGGFAEC 535

Query: 77  LCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNC 136
           LCGSP+LYQ GGRKPW+SINF+CAHDGF+LADLVTYN K+N +NGEDN DGENHN SWNC
Sbjct: 536 LCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKKYNSSNGEDNRDGENHNLSWNC 595

Query: 137 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 196
           G+EGEF  +SVK+LRKRQMRNFFV LMVSQGVPM  MGDEYGHTKGGNNNTYCHD+Y+NY
Sbjct: 596 GEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDHYVNY 655

Query: 197 FRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 256
           FRWDK EE SSD  RFCSLMT FR +CESLGL DFPT +RL WHGH PGKPDW+E SRFV
Sbjct: 656 FRWDKKEE-SSDLQRFCSLMTKFRKQCESLGLADFPTAQRLHWHGHQPGKPDWSETSRFV 715

Query: 257 AFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           AF+  D  K E+Y+AFNASHLP  V LPERPGYRW+PLVDTG
Sbjct: 716 AFSTKDETKGEIYVAFNASHLPAVVGLPERPGYRWEPLVDTG 756

[Close]

BLAST of Clc06G15110 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 5.6e-140
Identity = 223/273 (81.68%), Postives = 248/273 (90.84%), Query Frame = 0


Query: 25  ELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLY 84
           +LIAEAWDAGGLYQVG FPHWG+WSEWNGK+RD+VRQFIKGTDGFSGAFAECLCGSPNLY
Sbjct: 462 KLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLY 521

Query: 85  QGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVS 144
           Q GGRKPW+SINFICAHDGF+LADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F S
Sbjct: 522 Q-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFAS 581

Query: 145 ISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLEE 204
           ISVK+LRKRQMRNFFV LMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NYFRWDK EE
Sbjct: 582 ISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEE 641

Query: 205 SSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSV 264
           + SDF RFC ++  FR ECESLGLNDFPT +RLQWHG +P  P+W+E SRFVAF+LVDSV
Sbjct: 642 AHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSV 701

Query: 265 KREVYIAFNASHLPVTVMLPERPGYRWDPLVDT 298
           K+E+Y+AFN SHL   V LP RPGYRW+P VDT
Sbjct: 702 KKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDT 733

[Close]

BLAST of Clc06G15110 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 286.6 bits (732), Expect = 3.3e-76
Identity = 140/278 (50.36%), Postives = 185/278 (66.55%), Query Frame = 0


Query: 25  ELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLY 84
           ++IAE WD GGLY VG FP+W  W+EWNG YRD VR+FIKG  G  G+FA  + GS +LY
Sbjct: 461 KIIAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLY 520

Query: 85  QGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVS 144
           Q   RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE  NDG N N+SWNCG EGE   
Sbjct: 521 QVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGD 580

Query: 145 ISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLEE 204
             +K LR RQM+NF + LM+SQG PM+LMGDEYGHT+ GNNN+Y HD  LN F+W +L+ 
Sbjct: 581 AHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDA 640

Query: 205 SSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEA-SRFVAFTLVDS 264
              +  RF S +  FR+    L   +F T   + WH     + +W  + S+F+AFTL D 
Sbjct: 641 KKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNSESKFLAFTLHDG 700

Query: 265 V-KREVYIAFNASHLPVTVMLPE-RPGYRWDPLVDTGI 300
           +  R++Y+AFNA    V  ++P+  PG +W  + DT +
Sbjct: 701 IGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNL 733

[Close]

BLAST of Clc06G15110 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 4.5e-73
Identity = 138/278 (49.64%), Postives = 183/278 (65.83%), Query Frame = 0


Query: 25  ELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLY 84
           ++IAE WD GGLY VG FP+W  W+EWNGKYRD +R+FIKG  G  G FA  + GS +LY
Sbjct: 478 KIIAEPWDCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLY 537

Query: 85  QGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVS 144
           Q   RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE   DG N N SWNCG EGE   
Sbjct: 538 QVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETND 597

Query: 145 ISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLEE 204
           ++V  LR RQM+NF V LM+SQG PM+LMGDEYGHT+ GNNN+Y HD  +N F+W++LE+
Sbjct: 598 LNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQ 657

Query: 205 SSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDW-TEASRFVAFTLVD- 264
                 RF S M  FR+    L  + F     + WH     +  W  + S+F+AFT+ D 
Sbjct: 658 RRDGHFRFFSEMIKFRHSNPILRRDRFLNKNDVTWH-----EDCWENQESKFLAFTVHDH 717

Query: 265 SVKREVYIAFNASHLPVTVMLPERPGYR-WDPLVDTGI 300
           +   ++Y+AFNA    V  ++P  P ++ W+ +VDT +
Sbjct: 718 NSGGDIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNL 750

[Close]

BLAST of Clc06G15110 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.0e-54
Identity = 108/197 (54.82%), Postives = 131/197 (66.50%), Query Frame = 0


Query: 25  ELIAEAWDAG-GLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNL 84
           +LIAE WD G G YQVG FP  G+W+EWNGKYRD VR + +G     G FA  L GS +L
Sbjct: 394 KLIAEPWDVGEGGYQVGNFP--GLWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDL 453

Query: 85  YQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFV 144
           Y+  GR+P  SINF+ AHDGF+L DLV+YN KHN ANGE+N DGE++N SWNCG EG   
Sbjct: 454 YEATGRRPSASINFVTAHDGFTLNDLVSYNDKHNEANGENNRDGESYNRSWNCGVEGPTD 513

Query: 145 SISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLE 204
              +  LR RQMRN +  LMVSQG PMI  GDE G T+ GNNN YC D+ L++  W  L 
Sbjct: 514 DPDILALRARQMRNMWATLMVSQGTPMIAHGDEIGRTQYGNNNVYCQDSELSWMDW-SLV 573

Query: 205 ESSSDFHRFCSLMTTFR 221
           + ++D   F    TT R
Sbjct: 574 DKNADLLAFARKATTLR 587

[Close]

BLAST of Clc06G15110 vs. ExPASy TrEMBL
Match: A0A5A7UQM5 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.2e-163
Identity = 266/282 (94.33%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 17  NNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 76
           N+  +   +LIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 477 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 536

Query: 77  LCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNC 136
           LCGSPNLYQGGGRKP NSINF+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 537 LCGSPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 596

Query: 137 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 196
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY
Sbjct: 597 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 656

Query: 197 FRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 256
           FRWDK+EES+SDFHRFCSLMTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV
Sbjct: 657 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 716

Query: 257 AFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           AFTLVDSVKREVYIAFNASHLPVTV LPERPGYRWDPLVDTG
Sbjct: 717 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTG 758

[Close]

BLAST of Clc06G15110 vs. ExPASy TrEMBL
Match: A0A5D3DXS8 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001770 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.2e-163
Identity = 266/282 (94.33%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 17  NNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 76
           N+  +   +LIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 156 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 215

Query: 77  LCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNC 136
           LCGSPNLYQGGGRKP NSINF+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 216 LCGSPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 275

Query: 137 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 196
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY
Sbjct: 276 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 335

Query: 197 FRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 256
           FRWDK+EES+SDFHRFCSLMTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV
Sbjct: 336 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 395

Query: 257 AFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           AFTLVDSVKREVYIAFNASHLPVTV LPERPGYRWDPLVDTG
Sbjct: 396 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTG 437

[Close]

BLAST of Clc06G15110 vs. ExPASy TrEMBL
Match: A0A1S3BVW8 (isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.2e-163
Identity = 266/282 (94.33%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 17  NNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 76
           N+  +   +LIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 477 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 536

Query: 77  LCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNC 136
           LCGSPNLYQGGGRKP NSINF+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 537 LCGSPNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 596

Query: 137 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 196
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY
Sbjct: 597 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 656

Query: 197 FRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 256
           FRWDK+EES+SDFHRFCSLMTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV
Sbjct: 657 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 716

Query: 257 AFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           AFTLVDSVKREVYIAFNASHLPVTV LPERPGYRWDPLVDTG
Sbjct: 717 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTG 758

[Close]

BLAST of Clc06G15110 vs. ExPASy TrEMBL
Match: A0A6J1EW54 (isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.0e-160
Identity = 265/300 (88.33%), Postives = 279/300 (93.00%), Query Frame = 0


Query: 1   MSSFAPVNKEALCSAFNNVEI--GAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDI 60
           +++ +P+    L    +N  I  G K LIAEAWDAGGLYQVGTFPHW VWSEWNGKYRDI
Sbjct: 460 LTTGSPLGNPPLIDMISNDPILQGVK-LIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDI 519

Query: 61  VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNL 120
           VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGF+LADLVTYNSKHNL
Sbjct: 520 VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNL 579

Query: 121 ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYG 180
           ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYG
Sbjct: 580 ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYG 639

Query: 181 HTKGGNNNTYCHDNYLNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQ 240
           HTKGGNNNTYCHDNY+NYFRWDK+EESSSDFHRFC LMTTFRYECESLGLNDFPT +RLQ
Sbjct: 640 HTKGGNNNTYCHDNYINYFRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQ 699

Query: 241 WHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           WHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHLPVTV LPERPGY+W+PLVDTG
Sbjct: 700 WHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTG 758

[Close]

BLAST of Clc06G15110 vs. ExPASy TrEMBL
Match: A0A6J1ICQ3 (isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.5e-159
Identity = 264/300 (88.00%), Postives = 277/300 (92.33%), Query Frame = 0


Query: 1   MSSFAPVNKEALCSAFNNVEI--GAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDI 60
           +++ +P+    L    +N  I  G K LIAEAWDAGGLYQVGTFPHW VWSEWNGKYRDI
Sbjct: 460 LTTGSPLGNPPLIDMISNDPILQGVK-LIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDI 519

Query: 61  VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNL 120
           VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGF+LADLVTYNSKHNL
Sbjct: 520 VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNL 579

Query: 121 ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYG 180
           ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYG
Sbjct: 580 ANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYG 639

Query: 181 HTKGGNNNTYCHDNYLNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQ 240
           HTKGGNNNTYCHDNY+NYF WDK+EESSSDFHRFC LMTTFRYECESLGLNDFPT +RLQ
Sbjct: 640 HTKGGNNNTYCHDNYINYFLWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQ 699

Query: 241 WHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTG 299
           WHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHLPVTV LPER GYRW+PLVDTG
Sbjct: 700 WHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHLPVTVTLPERLGYRWEPLVDTG 758

[Close]

BLAST of Clc06G15110 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 498.4 bits (1282), Expect = 4.0e-141
Identity = 223/273 (81.68%), Postives = 248/273 (90.84%), Query Frame = 0


Query: 25  ELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLY 84
           +LIAEAWDAGGLYQVG FPHWG+WSEWNGK+RD+VRQFIKGTDGFSGAFAECLCGSPNLY
Sbjct: 462 KLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLY 521

Query: 85  QGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVS 144
           Q GGRKPW+SINFICAHDGF+LADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F S
Sbjct: 522 Q-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFAS 581

Query: 145 ISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLEE 204
           ISVK+LRKRQMRNFFV LMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NYFRWDK EE
Sbjct: 582 ISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEE 641

Query: 205 SSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSV 264
           + SDF RFC ++  FR ECESLGLNDFPT +RLQWHG +P  P+W+E SRFVAF+LVDSV
Sbjct: 642 AHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSV 701

Query: 265 KREVYIAFNASHLPVTVMLPERPGYRWDPLVDT 298
           K+E+Y+AFN SHL   V LP RPGYRW+P VDT
Sbjct: 702 KKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDT 733

[Close]

BLAST of Clc06G15110 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 286.6 bits (732), Expect = 2.4e-77
Identity = 140/278 (50.36%), Postives = 185/278 (66.55%), Query Frame = 0


Query: 25  ELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLY 84
           ++IAE WD GGLY VG FP+W  W+EWNG YRD VR+FIKG  G  G+FA  + GS +LY
Sbjct: 461 KIIAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLY 520

Query: 85  QGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVS 144
           Q   RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE  NDG N N+SWNCG EGE   
Sbjct: 521 QVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGD 580

Query: 145 ISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLEE 204
             +K LR RQM+NF + LM+SQG PM+LMGDEYGHT+ GNNN+Y HD  LN F+W +L+ 
Sbjct: 581 AHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDA 640

Query: 205 SSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEA-SRFVAFTLVDS 264
              +  RF S +  FR+    L   +F T   + WH     + +W  + S+F+AFTL D 
Sbjct: 641 KKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNSESKFLAFTLHDG 700

Query: 265 V-KREVYIAFNASHLPVTVMLPE-RPGYRWDPLVDTGI 300
           +  R++Y+AFNA    V  ++P+  PG +W  + DT +
Sbjct: 701 IGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNL 733

[Close]

BLAST of Clc06G15110 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 126.7 bits (317), Expect = 3.1e-29
Identity = 94/295 (31.86%), Postives = 142/295 (48.14%), Query Frame = 0


Query: 25  ELIAEAWDAGGLY-QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNL 84
           +LIA+ WD   +  +   FPHW  W+E N +Y   VR F++G  G     A  +CGS ++
Sbjct: 563 KLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDV 622

Query: 85  YQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFV 144
           +   GR P  S N+I  + G SL D+V++ S   LA+            SWNCG+EG   
Sbjct: 623 FT-DGRGPAFSFNYISRNSGLSLVDIVSF-SGPELAS----------ELSWNCGEEGATN 682

Query: 145 SISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLE 204
             +V + R +Q+RNF     +S GVP++ MGDE G +  G+            F W+ L 
Sbjct: 683 KSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESR----KPFDWNLLA 742

Query: 205 ES-SSDFHRFCSLMTTFR-YECESLGLNDFPTDERLQWHGHSPGKPDWTE-ASRFVAFTL 264
            +  +   +F S MT+ R    +     DF   E + W+ +    P W + AS+F+A  +
Sbjct: 743 SAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEI 802

Query: 265 VDSVKRE---------------VYIAFNASHLPVTVMLPERP-GYRWDPLVDTGI 300
               + E               ++I FNAS  P +V+LP  P G +W  LVDT +
Sbjct: 803 KSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTAL 840

[Close]

BLAST of Clc06G15110 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 126.7 bits (317), Expect = 3.1e-29
Identity = 94/295 (31.86%), Postives = 142/295 (48.14%), Query Frame = 0


Query: 25  ELIAEAWDAGGLY-QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNL 84
           +LIA+ WD   +  +   FPHW  W+E N +Y   VR F++G  G     A  +CGS ++
Sbjct: 563 KLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDV 622

Query: 85  YQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFV 144
           +   GR P  S N+I  + G SL D+V++ S   LA+            SWNCG+EG   
Sbjct: 623 FT-DGRGPAFSFNYISRNSGLSLVDIVSF-SGPELAS----------ELSWNCGEEGATN 682

Query: 145 SISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKLE 204
             +V + R +Q+RNF     +S GVP++ MGDE G +  G+            F W+ L 
Sbjct: 683 KSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESR----KPFDWNLLA 742

Query: 205 ES-SSDFHRFCSLMTTFR-YECESLGLNDFPTDERLQWHGHSPGKPDWTE-ASRFVAFTL 264
            +  +   +F S MT+ R    +     DF   E + W+ +    P W + AS+F+A  +
Sbjct: 743 SAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEI 802

Query: 265 VDSVKRE---------------VYIAFNASHLPVTVMLPERP-GYRWDPLVDTGI 300
               + E               ++I FNAS  P +V+LP  P G +W  LVDT +
Sbjct: 803 KSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTAL 840

The following BLAST results are available for this feature:

BLAST of Clc06G15110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879251.1	6.3e-165	95.39	isoamylase 1, chloroplastic [Benincasa hispida]	[more]
TYK28533.1	4.5e-163	94.33	isoamylase 1 [Cucumis melo var. makuwa]	[more]
XP_008453530.1	4.5e-163	94.33	PREDICTED: isoamylase 1, chloroplastic [Cucumis melo] >KAA0058172.1 isoamylase 1...	[more]
XP_004149676.1	8.5e-162	93.64	isoamylase 1, chloroplastic [Cucumis sativus] >KAE8653176.1 hypothetical protein...	[more]
KAG7021674.1	6.1e-160	88.33	Isoamylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Clc06G15110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D0TZF0	4.3e-140	79.08	Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=...	[more]
O04196	5.6e-140	81.68	Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1	[more]
Q9M0S5	3.3e-76	50.36	Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2	[more]
B9G434	4.5e-73	49.64	Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=...	[more]
P0A4Y5	8.0e-54	54.82	Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935...	[more]

BLAST of Clc06G15110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UQM5	2.2e-163	94.33	Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 ...	[more]
A0A5D3DXS8	2.2e-163	94.33	Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001770 ...	[more]
A0A1S3BVW8	2.2e-163	94.33	isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1	[more]
A0A6J1EW54	3.0e-160	88.33	isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 S...	[more]
A0A6J1ICQ3	9.5e-159	88.00	isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=...	[more]

BLAST of Clc06G15110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39930.1	4.0e-141	81.68	isoamylase 1	[more]
AT4G09020.1	2.4e-77	50.36	isoamylase 3	[more]
AT1G03310.2	3.1e-29	31.86	debranching enzyme 1	[more]
AT1G03310.1	3.1e-29	31.86	debranching enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 6..234 e-value: 2.9E-89 score: 302.0
None	No IPR available	PANTHER	PTHR43002	GLYCOGEN DEBRANCHING ENZYME	coord: 21..298
None	No IPR available	PANTHER	PTHR43002:SF1	ISOAMYLASE 1, CHLOROPLASTIC	coord: 21..298
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 236..300
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 236..302 e-value: 2.7E-21 score: 77.8
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc06G15110.1	Clc06G15110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005977	glycogen metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0019156	isoamylase activity