Clc06G15100 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc06G15100
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	isoamylase 1, chloroplastic
Location	ClcChr06: 26043653 .. 26045870 (+)
RNA-Seq Expression	Clc06G15100
Synteny	Clc06G15100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGTAGTATTCAATCACACAGCCGAGGGGAATGAAAATGGCCCCACTATTTCTTTCAGAGGTGTTGATAATAATGTGTATTACATGCTTGCACCAATGGTATGTAACTCTAGGATTATTAATTTTTCTCCCTCGCATTTTATTTAGAGGAAAATAACATATCATCAAGATAAGATGGAAGAATGATCTTTCAATTTCTTAATTCAGCTTTCTGCAAAGTTTATATTATTTTATCTATATGAGATTTTCATATGTTTCTCAGAAAAAAAGAAAAGAAAAATAAAAGAAAAATAAAGAAAATAATAATAATAAATAAATAAATAAAAATCTATACGAGATTTTTTTTGTATTCAACTGCGTGAAACTTATGTTGGCTTCTTTTTTAGCACTAGTTCCTTGGTCAATGTCAAACCCCTAATTTCCCTGCTATCTATTTTAAAGGTATCGAGGATGGAAAAACCCAAGCAAGTCACTACTCACTATTGAAAAGAAAGAAAAGATAAATTCTCCCCTTTAGAATTCATAAATGCTCAGATGGAGAAATGTTCAGAAACTAACTACCGCTTCTAATTTCTAATTCTTTATATATGTTTACGGCATTGATGTGTGCATTCAAATTACAGAGCCTTGGCATTCAGTATATCTCATACTTACTCAGTTACTCTCGATGGCTGTTTTAAATTTAAACCGGTAGGTATATTACCCATGTGCTAAAATTTAAAGCGGAGATTAAGCAGCAGTTATGGCTTAGACTTTTTTTCTTCCCTATCTTTCTTCTTCGTTATTATTATTATTATGTTTGTCTCTTCTTTTTCAATTATTTGATGTAATCATATCTTAACCATGGTTTTTCAGGGAGAGTTCTATAATTATTCAGGATGTGGGAAACATTTAACTGCAATCATCCAGCATGTACGTATGTTCATTGTGGACTGCTTAAGGTAGATGTACTATAGTCGTGGATACCATATTTCTGGCACTTGTTGGTTTCAATTTATTTAAAACACATGGAAAGAGGTATCTCAAAAGGAGAAGAATGATTAAATGTATACCTTTGAGAAAAAAATTACAGGGAACTCAACAAAGTAACAGCAGAATATACCAAATCTAAAACAAGGATATAAATTGGGAATGCAAAAGAGAAGGAAATAGAATATAAATCTTTTTAGAGAGAGAGAGGCCCCACTGTAAAACTCCTTATAGATAGATACATAGAGACAGGTATAAACCCAGTCGTAGCCAACACCTACTTCCATGGATTCTCAACTCTAAATATCCTTCTACTTCCCTTGAGCTAGATATTCCACAAATAGCTAAAACGGCTTTGGACCAAGGAGGCCTCTCCCTTGTTTCAAACAGTGAGTGAAAGAAGATCTTCTCCATCATCAACAACATCACCCTTAAACATGCCAACACACATCAAAAGTCTAAAAGAATCTGTCTCAAATCGAGTAAAATTGACAGCCCCATGAAACCCGTAACTGACTATGAAAATAGTCACACAAAACATTCCAGCCCTAATAACAAAGAAGACTGCTGCCCTTTGAATATGATCAGTAGGATATTATGCATGTCCTGTTTTCTAAATTTGAAATATTCTACATAAGCTAGATTATTGCATCTTATTGTTGGTTAGTGGTCTATCAACTCTTTTGGCCTTACCTTTGCGATCCTTTCCCCAATACCTCCCTCCTCACTGTAAGGAGGGCTGAGATGACAAGTTCATGCCTTTAATCATTCTCATCTTCTTAAGGTTCTCTCCTTGGAAGTAATTTTGTTCTTCAAGAAATGTACCGACTGACCACTGTACAAGTGCACTTTTTTGTACTTGGCTATTCCCATTCACTGACAGCACATCATGCATTTTCAGACTAAATTTATTTGGTTTTTGAGATGCAAACTTTTGGCCACAGATATTGGGTGACTGAAACTGCACGTGGATGGGTTTCGCTTGTACCTTGCTTCTATAATGACCAGAGGTAGCAGGTTATTAGCTGATCTTTTTGTCTGTATGCTTGATTTTTGTATTGACACCTTATCTCATTATTCATCCTTTTCCTCTTTTGTGGACAGTCTGTGGGATGCAGTAAATGTGTATGGAAATCCAATAGAAGGTGACGTACTCACAACTGGTTCTCCTCTTGGCAATACCTCCATGATTGACATGATAAGTAATGACCCAGTACTTCAAGGAGTGAAGGTGTTTACCCCTTAA

mRNA sequence

ATGGATGTAGTATTCAATCACACAGCCGAGGGGAATGAAAATGGCCCCACTATTTCTTTCAGAGGTGTTGATAATAATGTGTATTACATGCTTGCACCAATGGGAGAGTTCTATAATTATTCAGGATGTGGGAAACATTTAACTGCAATCATCCAGCATGTACGTATGTTCATTGTGGACTGCTTAAGATATTGGGTGACTGAAACTGCACGTGGATGGGTTTCGCTTGTACCTTGCTTCTATAATGACCAGAGTCTGTGGGATGCAGTAAATGTGTATGGAAATCCAATAGAAGGTGACGTACTCACAACTGGTTCTCCTCTTGGCAATACCTCCATGATTGACATGATAAGTAATGACCCAGTACTTCAAGGAGTGAAGGTGTTTACCCCTTAA

Coding sequence (CDS)

ATGGATGTAGTATTCAATCACACAGCCGAGGGGAATGAAAATGGCCCCACTATTTCTTTCAGAGGTGTTGATAATAATGTGTATTACATGCTTGCACCAATGGGAGAGTTCTATAATTATTCAGGATGTGGGAAACATTTAACTGCAATCATCCAGCATGTACGTATGTTCATTGTGGACTGCTTAAGATATTGGGTGACTGAAACTGCACGTGGATGGGTTTCGCTTGTACCTTGCTTCTATAATGACCAGAGTCTGTGGGATGCAGTAAATGTGTATGGAAATCCAATAGAAGGTGACGTACTCACAACTGGTTCTCCTCTTGGCAATACCTCCATGATTGACATGATAAGTAATGACCCAGTACTTCAAGGAGTGAAGGTGTTTACCCCTTAA

Protein sequence

MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVDCLRYWVTETARGWVSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMISNDPVLQGVKVFTP

Homology

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BLAST of Clc06G15100 vs. NCBI nr
Match: TYK28533.1 (isoamylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 200.7 bits (509), Expect = 7.9e-48
Identity = 99/130 (76.15%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 36  MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 95

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNVYGNPIEGD+LTTGSPLGN  +IDMIS
Sbjct: 96  CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 155

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 156 NDPVLQGVKL 165

[Close]

BLAST of Clc06G15100 vs. NCBI nr
Match: XP_008453530.1 (PREDICTED: isoamylase 1, chloroplastic [Cucumis melo] >KAA0058172.1 isoamylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 200.7 bits (509), Expect = 7.9e-48
Identity = 99/130 (76.15%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 357 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 416

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNVYGNPIEGD+LTTGSPLGN  +IDMIS
Sbjct: 417 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 476

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 477 NDPVLQGVKL 486

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BLAST of Clc06G15100 vs. NCBI nr
Match: XP_038879251.1 (isoamylase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 199.5 bits (506), Expect = 1.8e-47
Identity = 99/130 (76.15%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 360 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 419

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNV+GNPIEGDVLTTGSPLGN  +IDMIS
Sbjct: 420 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVFGNPIEGDVLTTGSPLGNPPLIDMIS 479

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 480 NDPVLQGVKL 489

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BLAST of Clc06G15100 vs. NCBI nr
Match: XP_004149676.1 (isoamylase 1, chloroplastic [Cucumis sativus] >KAE8653176.1 hypothetical protein Csa_019536 [Cucumis sativus])

HSP 1 Score: 198.7 bits (504), Expect = 3.0e-47
Identity = 98/130 (75.38%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 357 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 416

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNVYG+PIEGD+LTTGSPLGN  +IDMIS
Sbjct: 417 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGSPIEGDILTTGSPLGNPPLIDMIS 476

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 477 NDPVLQGVKL 486

[Close]

BLAST of Clc06G15100 vs. NCBI nr
Match: XP_022134724.1 (isoamylase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 195.3 bits (495), Expect = 3.3e-46
Identity = 97/130 (74.62%), Postives = 101/130 (77.69%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGP  SFRGVDN+VYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 357 MDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 416

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNVYGNPIEGDVLTTGSPLGN  +IDMIS
Sbjct: 417 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 476

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 477 NDPVLQGVKL 486

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BLAST of Clc06G15100 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 4.4e-41
Identity = 82/130 (63.08%), Postives = 93/130 (71.54%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNE GP +SFRG+DN+ YYMLAP GEFYNYSGCG         VR FIVD
Sbjct: 356 MDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYNYSGCGNTFNCNHPVVREFIVD 415

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWD VNVYG+P+EGD+ TTG+PL    +IDMIS
Sbjct: 416 CLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVEGDMTTTGTPLATPPLIDMIS 475

Query: 121 NDPVLQGVKV 129
           NDP+L  VK+
Sbjct: 476 NDPILGDVKL 485

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BLAST of Clc06G15100 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.3e-40
Identity = 82/130 (63.08%), Postives = 93/130 (71.54%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVV NHTAEGNE GP  SFRGVDN+VYYMLAP GEFYNYSGCG         VR FI+D
Sbjct: 334 MDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILD 393

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDA NVYG  +EGD+LTTG+P+    +IDMIS
Sbjct: 394 CLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMIS 453

Query: 121 NDPVLQGVKV 129
           NDP+L+GVK+
Sbjct: 454 NDPILRGVKL 463

[Close]

BLAST of Clc06G15100 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 5.4e-15
Identity = 50/129 (38.76%), Postives = 66/129 (51.16%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGP-TISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIV 60
           +DVV+NHT E ++  P T SFRG+DN VYYML P  +  N+SGCG  L      V   I+
Sbjct: 351 LDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELIL 410

Query: 61  DCLRYWVTETARGWVSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMISN 120
           D LR+WVTE           ++ D   +D  +V          T GSPL    +I  I+ 
Sbjct: 411 DSLRHWVTE-----------YHVDGFRFDLASVLCR------ATDGSPLSAPPLIRAIAK 462

Query: 121 DPVLQGVKV 129
           D VL   K+
Sbjct: 471 DSVLSRCKI 462

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BLAST of Clc06G15100 vs. ExPASy Swiss-Prot
Match: B2VJR7 (Glycogen debranching enzyme OS=Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99) OX=465817 GN=glgX PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 7.8e-14
Identity = 34/68 (50.00%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           +DVVFNH+AE +ENGPT+S RG+DN  YY L   G + N++GCG     +        +D
Sbjct: 259 LDVVFNHSAELDENGPTLSMRGIDNPTYYWLDGQGNYDNWTGCGNTQNLVHPEAVASTLD 318

Query: 61  CLRYWVTE 69
           CLRYWV E
Sbjct: 319 CLRYWVEE 326

[Close]

BLAST of Clc06G15100 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.1e-12
Identity = 37/70 (52.86%), Postives = 45/70 (64.29%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMG-EFY-NYSGCGKHLTAIIQHVRMFI 60
           +DVV+NHTAEGN  GPTI+FRG+DN  YY L      FY +++G G  L A   H    I
Sbjct: 284 LDVVYNHTAEGNHLGPTINFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLI 343

Query: 61  VDCLRYWVTE 69
           +D LRYWV E
Sbjct: 344 MDSLRYWVIE 353

[Close]

BLAST of Clc06G15100 vs. ExPASy TrEMBL
Match: A0A5A7UQM5 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.8e-48
Identity = 99/130 (76.15%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 357 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 416

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNVYGNPIEGD+LTTGSPLGN  +IDMIS
Sbjct: 417 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 476

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 477 NDPVLQGVKL 486

[Close]

BLAST of Clc06G15100 vs. ExPASy TrEMBL
Match: A0A5D3DXS8 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001770 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.8e-48
Identity = 99/130 (76.15%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 36  MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 95

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNVYGNPIEGD+LTTGSPLGN  +IDMIS
Sbjct: 96  CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 155

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 156 NDPVLQGVKL 165

[Close]

BLAST of Clc06G15100 vs. ExPASy TrEMBL
Match: A0A1S3BVW8 (isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.8e-48
Identity = 99/130 (76.15%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 357 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 416

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNVYGNPIEGD+LTTGSPLGN  +IDMIS
Sbjct: 417 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 476

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 477 NDPVLQGVKL 486

[Close]

BLAST of Clc06G15100 vs. ExPASy TrEMBL
Match: A0A6J1BZK5 (isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.6e-46
Identity = 97/130 (74.62%), Postives = 101/130 (77.69%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGP  SFRGVDN+VYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 357 MDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 416

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDAVNVYGNPIEGDVLTTGSPLGN  +IDMIS
Sbjct: 417 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 476

Query: 121 NDPVLQGVKV 129
           NDPVLQGVK+
Sbjct: 477 NDPVLQGVKL 486

[Close]

BLAST of Clc06G15100 vs. ExPASy TrEMBL
Match: A0A6J1ICQ3 (isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 8.0e-46
Identity = 95/130 (73.08%), Postives = 99/130 (76.15%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVVFNHTAEGNENGP  SFRGVDNNVYYMLAPMGEFYNYSGCG         VR FIVD
Sbjct: 357 MDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 416

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWV E    G+   L        SLWDAVNVYGNPIEGD LTTGSPLGN  +IDMIS
Sbjct: 417 CLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMIS 476

Query: 121 NDPVLQGVKV 129
           NDP+LQGVK+
Sbjct: 477 NDPILQGVKL 486

[Close]

BLAST of Clc06G15100 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 167.2 bits (422), Expect = 9.1e-42
Identity = 82/130 (63.08%), Postives = 93/130 (71.54%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIVD 60
           MDVV NHTAEGNE GP  SFRGVDN+VYYMLAP GEFYNYSGCG         VR FI+D
Sbjct: 334 MDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILD 393

Query: 61  CLRYWVTET-ARGW-VSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMIS 120
           CLRYWVTE    G+   L        SLWDA NVYG  +EGD+LTTG+P+    +IDMIS
Sbjct: 394 CLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMIS 453

Query: 121 NDPVLQGVKV 129
           NDP+L+GVK+
Sbjct: 454 NDPILRGVKL 463

[Close]

BLAST of Clc06G15100 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 82.0 bits (201), Expect = 3.8e-16
Identity = 50/129 (38.76%), Postives = 66/129 (51.16%), Query Frame = 0


Query: 1   MDVVFNHTAEGNENGP-TISFRGVDNNVYYMLAPMGEFYNYSGCGKHLTAIIQHVRMFIV 60
           +DVV+NHT E ++  P T SFRG+DN VYYML P  +  N+SGCG  L      V   I+
Sbjct: 351 LDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELIL 410

Query: 61  DCLRYWVTETARGWVSLVPCFYNDQSLWDAVNVYGNPIEGDVLTTGSPLGNTSMIDMISN 120
           D LR+WVTE           ++ D   +D  +V          T GSPL    +I  I+ 
Sbjct: 411 DSLRHWVTE-----------YHVDGFRFDLASVLCR------ATDGSPLSAPPLIRAIAK 462

Query: 121 DPVLQGVKV 129
           D VL   K+
Sbjct: 471 DSVLSRCKI 462

The following BLAST results are available for this feature:

BLAST of Clc06G15100 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK28533.1	7.9e-48	76.15	isoamylase 1 [Cucumis melo var. makuwa]	[more]
XP_008453530.1	7.9e-48	76.15	PREDICTED: isoamylase 1, chloroplastic [Cucumis melo] >KAA0058172.1 isoamylase 1...	[more]
XP_038879251.1	1.8e-47	76.15	isoamylase 1, chloroplastic [Benincasa hispida]	[more]
XP_004149676.1	3.0e-47	75.38	isoamylase 1, chloroplastic [Cucumis sativus] >KAE8653176.1 hypothetical protein...	[more]
XP_022134724.1	3.3e-46	74.62	isoamylase 1, chloroplastic [Momordica charantia]	[more]

BLAST of Clc06G15100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D0TZF0	4.4e-41	63.08	Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=...	[more]
O04196	1.3e-40	63.08	Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1	[more]
Q9M0S5	5.4e-15	38.76	Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2	[more]
B2VJR7	7.8e-14	50.00	Glycogen debranching enzyme OS=Erwinia tasmaniensis (strain DSM 17950 / CFBP 717...	[more]
P0A4Y5	1.1e-12	52.86	Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935...	[more]

BLAST of Clc06G15100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UQM5	3.8e-48	76.15	Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 ...	[more]
A0A5D3DXS8	3.8e-48	76.15	Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001770 ...	[more]
A0A1S3BVW8	3.8e-48	76.15	isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1	[more]
A0A6J1BZK5	1.6e-46	74.62	isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 ...	[more]
A0A6J1ICQ3	8.0e-46	73.08	isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=...	[more]

BLAST of Clc06G15100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39930.1	9.1e-42	63.08	isoamylase 1	[more]
AT4G09020.1	3.8e-16	38.76	isoamylase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..130 e-value: 6.4E-28 score: 99.6
None	No IPR available	PANTHER	PTHR43002:SF1	ISOAMYLASE 1, CHLOROPLASTIC	coord: 1..128
None	No IPR available	PANTHER	PTHR43002	GLYCOGEN DEBRANCHING ENZYME	coord: 1..128
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc06G15100.1	Clc06G15100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005977	glycogen metabolic process
molecular_function	GO:0019156	isoamylase activity