Clc06G13980 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc06G13980
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	Proteinase inhibitor 1
Location	ClcChr06: 24917519 .. 24918054 (-)
RNA-Seq Expression	Clc06G13980
Synteny	Clc06G13980

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGATATTCCTTTTCCTGGTAAGCTTAATTCAAAACTTTTAATCGTTCTTCTAAACCTTTCCAAAATATTTATAAATCCACGTTTGTTTTTCTATAGAGAAAGAATAGACAATCTTAATTATATTTTTGTAGTGTAATTTCAACCTAAAATGATCATAGTATTTATTTACTTTTGCCAAACAAATGTCTTTAACTTTGACAAAAAGATGTTGGTTTTCAAAAGTTGTTTGAATCTAGTCCAATATTTAGAGATGAGTTTTAAAACTAGTTATTGATTTGCTACACATTTGTAAGTAGTATGTACTAATTATTGAAAAATTAATACGTTGAACTAGGAAAAGCATCATGGCCAGAACTTGTGGGAATTGAAGCTGAAATTTCAAAGCATATCATACTGAAAGAGAATCCTCGTGTGAAAATTATTGAAATCATATTAGCTGGTAGTCCAGTGACTCTGGACTTGAGGGAGGACAGAGTTCGAATCTTTGTGAACATACGAAACGTTGCAGTTGAAATACCAAAGATTGGCTAA

mRNA sequence

ATGGCTGATATTCCTTTTCCTGGTAAGCTTAATTCAAAACTTTTAATCGTTCTTCTAAACCTTTCCAAAATATTTATAAATCCACGAAAAGCATCATGGCCAGAACTTGTGGGAATTGAAGCTGAAATTTCAAAGCATATCATACTGAAAGAGAATCCTCGTGTGAAAATTATTGAAATCATATTAGCTGGTAGTCCAGTGACTCTGGACTTGAGGGAGGACAGAGTTCGAATCTTTGTGAACATACGAAACGTTGCAGTTGAAATACCAAAGATTGGCTAA

Coding sequence (CDS)

ATGGCTGATATTCCTTTTCCTGGTAAGCTTAATTCAAAACTTTTAATCGTTCTTCTAAACCTTTCCAAAATATTTATAAATCCACGAAAAGCATCATGGCCAGAACTTGTGGGAATTGAAGCTGAAATTTCAAAGCATATCATACTGAAAGAGAATCCTCGTGTGAAAATTATTGAAATCATATTAGCTGGTAGTCCAGTGACTCTGGACTTGAGGGAGGACAGAGTTCGAATCTTTGTGAACATACGAAACGTTGCAGTTGAAATACCAAAGATTGGCTAA

Protein sequence

MADIPFPGKLNSKLLIVLLNLSKIFINPRKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVEIPKIG

Homology

[Close]

BLAST of Clc06G13980 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 84.0 bits (206), Expect = 7.7e-13
Identity = 42/69 (60.87%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 25 FINPRKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRN 84
          F  P K  WPELVGI++ I++HII K+N  V+ +E ILAGS VT DLR DRVRIFVNI+ 
Sbjct: 4  FCPPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVNIKG 63

Query: 85 VAVEIPKIG 94
          + V+IP IG
Sbjct: 64 IIVQIPTIG 72

[Close]

BLAST of Clc06G13980 vs. NCBI nr
Match: KAG6571499.1 (Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 80.5 bits (197), Expect = 8.5e-12
Identity = 42/69 (60.87%), Postives = 47/69 (68.12%), Query Frame = 0


Query: 25 FINPRKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRN 84
          F  P K  WPELVGIEA I + II KENP V I++I LAGSPV LD    RVR+FVNI +
Sbjct: 4  FCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNIND 63

Query: 85 VAVEIPKIG 94
          V V IP  G
Sbjct: 64 VVVSIPWTG 72

[Close]

BLAST of Clc06G13980 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 78.2 bits (191), Expect = 4.2e-11
Identity = 35/66 (53.03%), Postives = 50/66 (75.76%), Query Frame = 0


Query: 28 PRKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAV 87
          P K  WPELVG++A  +K+II K+NP V+ + ++LAGS  T D+R DRV +FVNI ++ V
Sbjct: 7  PGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDLVV 66

Query: 88 EIPKIG 94
          E+PK+G
Sbjct: 67 EVPKVG 72

[Close]

BLAST of Clc06G13980 vs. NCBI nr
Match: MQL67652.1 (hypothetical protein [Streptomyces vinaceus])

HSP 1 Score: 77.8 bits (190), Expect = 5.5e-11
Identity = 32/64 (50.00%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 30 KASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVEI 89
          K SWPELVG+  +++K  I KENP++  + I+L G+P+TLD R DRVRIFVN   + +++
Sbjct: 18 KQSWPELVGVPGKVAKETIQKENPKLTQVVIVLIGTPITLDYRFDRVRIFVNYLGIVIQV 77

Query: 90 PKIG 94
          P++G
Sbjct: 78 PRVG 81

[Close]

BLAST of Clc06G13980 vs. NCBI nr
Match: XP_004247658.1 (wound-induced proteinase inhibitor 1 [Solanum lycopersicum])

HSP 1 Score: 77.4 bits (189), Expect = 7.2e-11
Identity = 39/64 (60.94%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 30  KASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVEI 89
           K SWPEL+G+ A+ +K II KENP V  ++I+L GSPVT D R  RVRI VNI NVAV I
Sbjct: 48  KLSWPELIGVPAQYAKGIIQKENPFVTDVQIVLNGSPVTADFRCFRVRIVVNILNVAVSI 107

Query: 90  PKIG 94
           P +G
Sbjct: 108 PVVG 111

[Close]

BLAST of Clc06G13980 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.0e-12
Identity = 36/76 (47.37%), Postives = 50/76 (65.79%), Query Frame = 0


Query: 18  LLNLSKIFINPRKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVR 77
           ++ L K F    K  WPEL+G+  +++K II KEN  +  + I+L GSPVTLD+R DRVR
Sbjct: 32  VIQLLKEFQCKGKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVR 91

Query: 78  IFVNIRNVAVEIPKIG 94
           +F NI    V+IP +G
Sbjct: 92  LFDNILGYVVDIPVVG 107

[Close]

BLAST of Clc06G13980 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 5.2e-12
Identity = 33/64 (51.56%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 30  KASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVEI 89
           K SWPEL+G  A+ +K II KENP++  +E +L GS  T DLR +RVR+FVN+ ++ V+ 
Sbjct: 56  KESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQT 115

Query: 90  PKIG 94
           PK+G
Sbjct: 116 PKVG 119

[Close]

BLAST of Clc06G13980 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 8.8e-12
Identity = 30/65 (46.15%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 29 RKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVE 88
          +K +WPEL+G+ A+ ++ II KEN ++  +  +L GSPVT D R +RVR+FVN+ +  V+
Sbjct: 30 KKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQ 89

Query: 89 IPKIG 94
          IP++G
Sbjct: 90 IPRVG 94

[Close]

BLAST of Clc06G13980 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.2e-11
Identity = 35/63 (55.56%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 30  KASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVEI 89
           K  WPEL+G+ A  +K II KENP +  I I+L GSPVT D R DRVR+FVNI    V+I
Sbjct: 48  KQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQI 107

Query: 90  PKI 93
           P++
Sbjct: 108 PRV 110

[Close]

BLAST of Clc06G13980 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.7e-11
Identity = 31/63 (49.21%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 30  KASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVEI 89
           K  WPEL+G+  +++K II KENP +  I I+L+GSP+TLD   DRVR+F NI    V++
Sbjct: 48  KQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQM 107

Query: 90  PKI 93
           P +
Sbjct: 108 PVV 110

[Close]

BLAST of Clc06G13980 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.0e-11
Identity = 35/66 (53.03%), Postives = 50/66 (75.76%), Query Frame = 0


Query: 28 PRKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAV 87
          P K  WPELVG++A  +K+II K+NP V+ + ++LAGS  T D+R DRV +FVNI ++ V
Sbjct: 7  PGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDLVV 66

Query: 88 EIPKIG 94
          E+PK+G
Sbjct: 67 EVPKVG 72

[Close]

BLAST of Clc06G13980 vs. ExPASy TrEMBL
Match: A0A3Q7IN73 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101256437 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 5.9e-11
Identity = 40/90 (44.44%), Postives = 59/90 (65.56%), Query Frame = 0


Query: 14 LLIVLLNLSKIFINP----------RKASWPELVGIEAEISKHIILKENPRVKIIEIILA 73
          L++  L LS I+I P          +K +WPEL+G+ A+++K II KEN R+  I  +L 
Sbjct: 6  LVVAFLLLSSIYIQPLTAQSICPGVKKDTWPELLGVPAKLAKRIIQKENRRLTNIPNVLN 65

Query: 74 GSPVTLDLREDRVRIFVNIRNVAVEIPKIG 94
          GSPVT DLR +RVR+FVN+ +  V+ P++G
Sbjct: 66 GSPVTKDLRCNRVRLFVNVLDYVVQTPRVG 95

[Close]

BLAST of Clc06G13980 vs. ExPASy TrEMBL
Match: A0A6N2C3W9 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_002136 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 5.9e-11
Identity = 40/90 (44.44%), Postives = 59/90 (65.56%), Query Frame = 0


Query: 14 LLIVLLNLSKIFINP----------RKASWPELVGIEAEISKHIILKENPRVKIIEIILA 73
          L++  L LS I+I P          +K +WPEL+G+ A+++K II KEN R+  I  +L 
Sbjct: 6  LVVAFLLLSSIYIQPLTAQSICPGVKKDTWPELLGVPAKLAKRIIQKENRRLTNIPNVLN 65

Query: 74 GSPVTLDLREDRVRIFVNIRNVAVEIPKIG 94
          GSPVT DLR +RVR+FVN+ +  V+ P++G
Sbjct: 66 GSPVTKDLRCNRVRLFVNVLDYVVQTPRVG 95

[Close]

BLAST of Clc06G13980 vs. ExPASy TrEMBL
Match: A0A1S3YJI6 (trypsin inhibitor 1-like OS=Nicotiana tabacum OX=4097 GN=LOC107776717 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.3e-10
Identity = 31/65 (47.69%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 29 RKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVE 88
          +K +WPEL+G+ A+ ++ II KENP++  +  +L GSPVT DLR +RVR+FVN+ ++ V+
Sbjct: 30 KKETWPELIGVPAKFAREIIQKENPKLTNVPSLLNGSPVTKDLRCNRVRLFVNVLDIVVQ 89

Query: 89 IPKIG 94
          +P++G
Sbjct: 90 LPRVG 94

[Close]

BLAST of Clc06G13980 vs. ExPASy TrEMBL
Match: A0A2G3BF90 (Proteinase inhibitor I-B OS=Capsicum chinense OX=80379 GN=BC332_25972 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.3e-10
Identity = 34/65 (52.31%), Postives = 50/65 (76.92%), Query Frame = 0


Query: 29 RKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAVE 88
          +K +WPEL+G+ A++++  I KEN R+  +  +L GSPVTLDLR DRVR+FVN+ +  VE
Sbjct: 10 KKDAWPELLGVPAKLARETIQKENRRLTNVPNVLNGSPVTLDLRCDRVRLFVNVLDFVVE 69

Query: 89 IPKIG 94
          IP++G
Sbjct: 70 IPRVG 74

[Close]

BLAST of Clc06G13980 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 65.5 bits (158), Expect = 2.6e-11
Identity = 33/66 (50.00%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 28 PRKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFVNIRNVAV 87
          PRK SWPEL G   + +  +I +ENP V    +IL GSPVT D R DRVR+FV+   + V
Sbjct: 6  PRKNSWPELTGTNGDYAAVVIERENPTVN-AAVILDGSPVTADFRCDRVRVFVDGNRIVV 65

Query: 88 EIPKIG 94
          + PK G
Sbjct: 66 KTPKSG 70

[Close]

BLAST of Clc06G13980 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.3 bits (129), Expect = 6.1e-08
Identity = 30/73 (41.10%), Postives = 47/73 (64.38%), Query Frame = 0


Query: 21 LSKIFINPRKASWPELVGIEAEISKHIILKENPRVKIIEIILAGSPVTLDLREDRVRIFV 80
          ++ I  +P K+SWPEL+G + E +K +I +ENP++K + IIL G+ V       RV ++V
Sbjct: 28 MASICEDPGKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWV 87

Query: 81 NIRNVAVEIPKIG 94
          N   + V+IP IG
Sbjct: 88 NDCGIVVQIPIIG 99

The following BLAST results are available for this feature:

BLAST of Clc06G13980 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571494.1	7.7e-13	60.87	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6571499.1	8.5e-12	60.87	Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KGN50840.1	4.2e-11	53.03	hypothetical protein Csa_004718 [Cucumis sativus]	[more]
MQL67652.1	5.5e-11	50.00	hypothetical protein [Streptomyces vinaceus]	[more]
XP_004247658.1	7.2e-11	60.94	wound-induced proteinase inhibitor 1 [Solanum lycopersicum]	[more]

BLAST of Clc06G13980 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00783	3.0e-12	47.37	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
P20076	5.2e-12	51.56	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
Q02214	8.8e-12	46.15	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P16231	1.2e-11	55.56	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P05118	5.7e-11	49.21	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]

BLAST of Clc06G13980 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KME7	2.0e-11	53.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]
A0A3Q7IN73	5.9e-11	44.44	Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101256437 PE=3 SV=1	[more]
A0A6N2C3W9	5.9e-11	44.44	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_002136 PE=3 SV=1	[more]
A0A1S3YJI6	1.3e-10	47.69	trypsin inhibitor 1-like OS=Nicotiana tabacum OX=4097 GN=LOC107776717 PE=3 SV=1	[more]
A0A2G3BF90	1.3e-10	52.31	Proteinase inhibitor I-B OS=Capsicum chinense OX=80379 GN=BC332_25972 PE=3 SV=1	[more]

BLAST of Clc06G13980 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	2.6e-11	50.00	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	6.1e-08	41.10	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 32..45 score: 50.0 coord: 58..69 score: 47.83 coord: 70..80 score: 58.1
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 30..93 e-value: 9.2E-21 score: 74.3
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 24..93
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 25..93 e-value: 1.2E-20 score: 75.7
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 24..93
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 26..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc06G13980.2	Clc06G13980.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity