Clc06G13300 (gene) Watermelon (cordophanus) v2

Overview
NameClc06G13300
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Descriptionomega-6 fatty acid desaturase, chloroplastic
LocationClcChr06: 24241860 .. 24246354 (+)
RNA-Seq ExpressionClc06G13300
SyntenyClc06G13300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGATGACAACGCCACTCTGCACACCAGTGGCGTAAGCTCGAAAAGGGCCACCCCTTTCAGAGAATTCTATCCACATCTTAAAGAACGATAGGCGAAGGATTTATACCCAATTTCTCATTTCAAATCGCAGCCTTGAATACTTAAGGAGATACCCATTAAGAATTCCCCCCCTTTCCACCGTCTATTTCCCTCTCATTCAAAGAGCCCAAGCAAGCTAAGGGGGCCTTCTTAACTGGGATTTCTGCAATTCTGCACATAATGGCTTGCAGACTTGCGGATTCCATGGTTCTTTTCAATGTATGTTTCCCGCCATGTCTTCTTTTTAAGCTTACCCAATTATGGTACTTGTGTTCTTCTTTTCTTATGGTTTTGGTTTGATTATCAGGGCCTCCATCAGAATTCAGCTCGAACCCAGAAAATTGCATCACATTATTCTTCAGGTATTTTTTCCTATCTTCCTTTCCAGTTTTGAGCCATTGAGTTATTTGAAGTTGTTTGAGAATTTGTAATCACTCGATAGACTGAATGGTCTAGCAACTGTTTGATACTGAAACTTTGAGAGTATATACGTTGAAGGTTCAATTCCATTGACTTTGTCACTCCTTGCTGTTGCAGACATGGTTTCTGTGATGAAATATTGAGTAGATTTGGAAACTAGATAGAAGTTAGAGTCCCACTCAGTTCGGGCAAATTAATCTTTCAAGTAAATTGATAATTTTTCATATCCTTATTCAACTCAAGCATGAATTTTATATACAATAAAATGAATTAATTGACCGGGAACCATCCAACATAGCTTAGATTTTGCATTTGTTACTTTATTCTCATAATGGGTTGCTCTAAGGTTAGTATTTTTTTTTTTCTACTTTCTTTTTTTAGCATAAATAATTTAGGTCCCCTTTAGTAATCATTTTGTTTTTGGTTTTTGGATTTTGAAAATTGTGCTTATTTCCTTACAGTTTCTTACTCATAGTTTTGTTTTTGGTTTTTGGTTTTTGGATCTTGAAGACTACTTTTTTTTAGTTTTCAAATTTTGGCTTTGATTTTGAAAACACTCCTAAAATGTAGACAACAAACCATAGCAACCACTAAATGGAATCACGTCTATAGGCTTAATTTTTAGAAACCAAAAATCAAAAATCAAATAGTTACAGACGAGAACTTAGATTTTCCATTGTCAATTTAGATGGTTCAAACATGCTTATTCCGATAGACAGGTATATTGCATGTGAAGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAAAACTATCCACTTCCTATGAAGAGGGCTGCAATTTTACGGGCAGTTGCTGTTCCAGTTGCACCACCAACCTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCAAGCAAATTGGAGAACCTCTTCCTGAAAATGTTAGGTTGAAAGATATTGTTGAGTCCCTTCCAAAAGAGGTACTTCATTTTGGTCTTTAACATCTGTCCTTGAGGCAATTGCATTTGCGTTTTTTTCTGTCAAATAAAATTTTGACGATGTTCTACTTCATGTTTATAGTTACTTACATAGCCTAGTTAGCTCATGTTCATGTTTCTTTTACTCATTTTATCTTTTTTTTTTTTTTTTTTTTTTTTAAAAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTATACATTAGGCCTTGTCATGATTGCAAAAGCTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACTGCAATAACAGGGGTTAGTCTTATTCAAATATTCTTTTGGGTTGCTTCCCTTTGTCTCTCTCTCTTCCTCTCATCCTTGTCGGACTTGCAACTCTGGCCACTGAAGTTACTTTCCTCCTCAAGTTCTTTGTTATAGGTCATGACTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGTATTAATAATCTCTCCTCTGTCTCTATCTCTCTTTGAAATACTTATTTTAGCTCTATCTTCCTGCATAAACATAGCTCAATATTGTTATGTCCTATAGTATAAAGAAATGTCTTATTGAGGTAGGATAACATCTAGTTTAGCAATGCAAAAGCCTTGATCAATAATATTGGGCAGAACAGTAGGAATGGATATTAATGTGTTATAAATTTATAATCCGTACCTTGATACTGCTAGGTAAACATGACATCATATTCTACTTACCACTGTTGAGCATGCAGTAATTCTTCCTTGTATCTTAGGATCAAGTTCATGGAATTTCTCCATATGTTTTGCATTTTGCCTAGAGTCAAATATCGTTGTGTTAGTTAAGCTTGAAATATTACATGTATGACTAAATAGGAACTCCATATTTTATAAGTTTGGCTTGTTACTTACATTTAAAAGATTTATGCCTTTCATCTTCTAGTTTTAGCTAAAGTTCCATTTCTGTTCTTATCCACGTATTTTATAGGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCGCCTCTTCTGCGTAAGGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTAAGTTAACCTTGGATGGTTCCTTTGCTAGAATAACTTGACTTTATATGAAGATCTCTTGTATGTGATTTTACCAAATTCTCTCTCTTTCTTTCATTAATATGCACCAAGTTGTTCTTATGCATAATAGGTTGTTGTGGCACTTCGATTTGAAAAAGTTCAGACCAAACGAAGTGAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACCGGGATTTTTGGATGGGTCAAGTTCTGGTTAATGCCATGGTTGGGCTACCACTTTTGGGTAACATCATTTGCTTGCCTCTTCTTATTACAGTGTCTCCTATGATCAATTCAGTCGATGATCTGTGTTGAATAGTGATTTGTTATGGTTCTCCATGATGTGACATGGAATTAACAAACAAGTTTACAATCAGAATTAGAGGATATATAGCATGGTAGACTCTAATAAAAATGAATCATATAACTTAGGTTACAGTATCCAAGTATCTTCGATATTGTTGAAACATTAATCTCAATGAAGCTTATATCTTGCACTAATATTCCTGAAGCAAATAAAAGAATGCTTGATATCATGCTTTCAAGATCATGAGACAAAGATATGATGTTTATGCCACTGTTGTTCATTCATAGAGGGACAATATTTTACTGTTCTTGTTCTGCACTATTTTGAAATTGGCCCAGATAAAATTTGTTGCTTTTGCTTATGTAAATTTAGATTCTATTTAAATTAATGATATAATTTATCTTTTAGCTTCTGTTTACCTACTTATGATGGTGTATCACTTTTTGTTTCTTGCTTAGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCTTCAGAAGAGTGGAACGCAGCTCAAGCGCAGCTCAATGGAACAGTTCATTGTGCTTATCCTCAGTGGTACTTAATAATTCATTTCAGTGTTTTTTCCCCTTCTCTTTTCTCTTCTCTTGCTTAATCTGACCCTAAGCTGATAAAAACGTGTGTTAGAAAAAAATTATGGCTCTGTTCGTGAGATCCTCTAGTCTTGAAATTGCTAGATAATTCATGTTAAATAAGATCTATTACCATAAATCATATTTGACTTAGCTTCTAATTTTATCTTCTCCCCTCCACAGGATCGAGATTCTTTGCCATGACATCAATGTGCACATTCCCCACCATGTTTCTCCAAGAATACCAAGTTACAACTTACGGGCAGCCCACAAGTCTCTTCAAGAAAATTGGGGAAAGGTAGTGGGATAAACCGAATGCATGTCTGCATTTTCTTTTCGATATAGTTTGGAATTTAGTTCTTGCAAAATCTAATGTCTGCGTTGTTTATTGCATATATTTTCCCAATTGATGCTAATAATCTAGTTCTTTTTCTTCCATCTTTGTTTCCCCTGCAACACATTCTATAGCATCTGAATGAGGCTTCATGGAATTGGCGTTTAATGAAGACGATCATGACGATATGTCATGTCTACAGTAAAGAGGAAAACTACATTGCTTTTGACCGACTCAATCAAGAAGATTCTTATCCCATTACATTCCTCAGGAAAGTTATGCCTGATCTTGCTTGATATTACCTCAACCTTCAGGAGCACAGACGCTTCTTTCTTTTGCTGTGCAATGTTTTTCTCCACAAATTTTGAGGAATGAATGGAAGGAAACTTGTTGGCCCGCCATATATTGAGTTGATGAATTTCTCTGTTTCATTTTCCAGTCTCTCTCTCCCGTTTTCTCACTAGGAAACTTTTCTTTTCTCCCCAAG

mRNA sequence

ATGAGTGATGACAACGCCACTCTGCACACCAGTGGCGTAAGCTCGAAAAGGGGCCTCCATCAGAATTCAGCTCGAACCCAGAAAATTGCATCACATTATTCTTCAGGTTCAATTCCATTGACTTTGTCACTCCTTGCTGTTGCAGACATGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAAAACTATCCACTTCCTATGAAGAGGGCTGCAATTTTACGGGCAGTTGCTGTTCCAGTTGCACCACCAACCTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCAAGCAAATTGGAGAACCTCTTCCTGAAAATGTTAGGTTGAAAGATATTGTTGAGTCCCTTCCAAAAGAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTATACATTAGGCCTTGTCATGATTGCAAAAGCTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACTGCAATAACAGGGTTCTTTGTTATAGGTCATGACTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCGCCTCTTCTGCGTAAGGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTTGTTGTGGCACTTCGATTTGAAAAAGTTCAGACCAAACGAAGTGAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACCGGGATTTTTGGATGGGTCAAGTTCTGGTTAATGCCATGGTTGGGCTACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCTTCAGAAGAGTGGAACGCAGCTCAAGCGCAGCTCAATGGAACAGTTCATTGTGCTTATCCTCAGTGGATCGAGATTCTTTGCCATGACATCAATGTGCACATTCCCCACCATGTTTCTCCAAGAATACCAAGTTACAACTTACGGGCAGCCCACAAGTCTCTTCAAGAAAATTGGGGAAAGCATCTGAATGAGGCTTCATGGAATTGGCGTTTAATGAAGACGATCATGACGATATGTCATGTCTACAGTAAAGAGGAAAACTACATTGCTTTTGACCGACTCAATCAAGAAGATTCTTATCCCATTACATTCCTCAGGAAAGTTATGCCTGATCTTGCTTGATATTACCTCAACCTTCAGGAGCACAGACGCTTCTTTCTTTTGCTGTGCAATGTTTTTCTCCACAAATTTTGAGGAATGAATGGAAGGAAACTTGTTGGCCCGCCATATATTGAGTTGATGAATTTCTCTGTTTCATTTTCCAGTCTCTCTCTCCCGTTTTCTCACTAGGAAACTTTTCTTTTCTCCCCAAG

Coding sequence (CDS)

ATGAGTGATGACAACGCCACTCTGCACACCAGTGGCGTAAGCTCGAAAAGGGGCCTCCATCAGAATTCAGCTCGAACCCAGAAAATTGCATCACATTATTCTTCAGGTTCAATTCCATTGACTTTGTCACTCCTTGCTGTTGCAGACATGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAAAACTATCCACTTCCTATGAAGAGGGCTGCAATTTTACGGGCAGTTGCTGTTCCAGTTGCACCACCAACCTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCAAGCAAATTGGAGAACCTCTTCCTGAAAATGTTAGGTTGAAAGATATTGTTGAGTCCCTTCCAAAAGAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTATACATTAGGCCTTGTCATGATTGCAAAAGCTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACTGCAATAACAGGGTTCTTTGTTATAGGTCATGACTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCGCCTCTTCTGCGTAAGGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTTGTTGTGGCACTTCGATTTGAAAAAGTTCAGACCAAACGAAGTGAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACCGGGATTTTTGGATGGGTCAAGTTCTGGTTAATGCCATGGTTGGGCTACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCTTCAGAAGAGTGGAACGCAGCTCAAGCGCAGCTCAATGGAACAGTTCATTGTGCTTATCCTCAGTGGATCGAGATTCTTTGCCATGACATCAATGTGCACATTCCCCACCATGTTTCTCCAAGAATACCAAGTTACAACTTACGGGCAGCCCACAAGTCTCTTCAAGAAAATTGGGGAAAGCATCTGAATGAGGCTTCATGGAATTGGCGTTTAATGAAGACGATCATGACGATATGTCATGTCTACAGTAAAGAGGAAAACTACATTGCTTTTGACCGACTCAATCAAGAAGATTCTTATCCCATTACATTCCTCAGGAAAGTTATGCCTGATCTTGCTTGA

Protein sequence

MSDDNATLHTSGVSSKRGLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMKRAAILRAVAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDRLNQEDSYPITFLRKVMPDLA
Homology
BLAST of Clc06G13300 vs. NCBI nr
Match: TYK03611.1 (omega-6 fatty acid desaturase [Cucumis melo var. makuwa])

HSP 1 Score: 880.6 bits (2274), Expect = 6.0e-252
Identity = 419/456 (91.89%), Postives = 435/456 (95.39%), Query Frame = 0

Query: 1   MSDDNATLHTSGVSSKRGLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIK 60
           MSD+NA L+TSG SSK G HQN ARTQKIASH S G++ +          GEILFQKKIK
Sbjct: 1   MSDNNAALNTSGASSKGGPHQNLARTQKIASHCSPGTLQV---------KGEILFQKKIK 60

Query: 61  RQNYPLPMKRAAILRAVAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIV 120
           RQNY LP+K+AAILRAVAVPVAPP+SSADSAEYRKQLSESYGF+QIGEPLP+NVRLKDIV
Sbjct: 61  RQNYLLPVKKAAILRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIV 120

Query: 121 ESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGH 180
           ESLPKEVFEINDVKAWKSVLVSVASY+LGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGH
Sbjct: 121 ESLPKEVFEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGH 180

Query: 181 DCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEF 240
           DCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEF
Sbjct: 181 DCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEF 240

Query: 241 DSAPLLRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWP 300
           DSAP+LRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWP
Sbjct: 241 DSAPILRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWP 300

Query: 301 LIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHC 360
           LIIYKTGI GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHC
Sbjct: 301 LIIYKTGILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHC 360

Query: 361 AYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT 420
           AYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT
Sbjct: 361 AYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT 420

Query: 421 ICHVYSKEENYIAFDRLNQEDSYPITFLRKVMPDLA 457
           +CHVYSKEENY+AFDRLN+EDSYPITFL+KVMPDLA
Sbjct: 421 VCHVYSKEENYVAFDRLNKEDSYPITFLKKVMPDLA 447

BLAST of Clc06G13300 vs. NCBI nr
Match: KAG6587828.1 (hypothetical protein SDJN03_16393, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 868.2 bits (2242), Expect = 3.1e-248
Identity = 415/473 (87.74%), Postives = 432/473 (91.33%), Query Frame = 0

Query: 1   MSDDNATLHTSGVSSKR-----------------GLHQNSARTQKIASHYSSGSIPLTLS 60
           MSD+NA LHTSG  SKR                 G HQN ARTQKIASH + G + +   
Sbjct: 1   MSDNNAALHTSGDGSKRATPSILSVLADSLFLFTGPHQNYARTQKIASHNAPGIMDV--- 60

Query: 61  LLAVADMGEILFQKKIKRQNYPLPMKRAAILRAVAVPVAPPTSSADSAEYRKQLSESYGF 120
                  G+I+FQKKIKRQNY LP+KRAAILRAVAVPVAPP+SSAD AEYRKQLSESYGF
Sbjct: 61  ------KGKIVFQKKIKRQNYLLPVKRAAILRAVAVPVAPPSSSADGAEYRKQLSESYGF 120

Query: 121 KQIGEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPL 180
           +QIGEPLPENVRLKDI+ESLPKEVFEINDVKAWKSVLVSVASYTLGL+MIAKAPWYLLPL
Sbjct: 121 EQIGEPLPENVRLKDIIESLPKEVFEINDVKAWKSVLVSVASYTLGLIMIAKAPWYLLPL 180

Query: 181 AWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKT 240
           AWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKT
Sbjct: 181 AWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKT 240

Query: 241 NMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKR 300
           NML+EDTAWHPVWKEEFDSAPLLRKAI+YGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKR
Sbjct: 241 NMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKR 300

Query: 301 VKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKS 360
           VKISLACVFAFMFIGWPLIIYKTGI GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK+
Sbjct: 301 VKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKA 360

Query: 361 SEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGK 420
           SEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHH+SPRIPSYNLRAAHKSLQENWGK
Sbjct: 361 SEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGK 420

Query: 421 HLNEASWNWRLMKTIMTICHVYSKEENYIAFDRLNQEDSYPITFLRKVMPDLA 457
           HLNEASWNWRLMKTIMTICHVYSKEENY+AFDRL  EDSYPITFL+KVMPDLA
Sbjct: 421 HLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLKPEDSYPITFLKKVMPDLA 464

BLAST of Clc06G13300 vs. NCBI nr
Match: XP_038880078.1 (omega-6 fatty acid desaturase, chloroplastic [Benincasa hispida])

HSP 1 Score: 866.3 bits (2237), Expect = 1.2e-247
Identity = 410/439 (93.39%), Postives = 420/439 (95.67%), Query Frame = 0

Query: 18  GLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMKRAAILRAV 77
           G HQN ARTQKIASH S G + +          GEILFQKKIKRQNY LP+KRAAILRAV
Sbjct: 14  GPHQNLARTQKIASHCSPGILQV---------KGEILFQKKIKRQNYLLPVKRAAILRAV 73

Query: 78  AVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAWK 137
           AVPVAPP+SSADSAEYRKQLSESYGF+QIGEPLP+NVRLKDIVESLPKEVFEINDVKAWK
Sbjct: 74  AVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDVKAWK 133

Query: 138 SVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 197
           SVLVSVASYTLGLVMIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV
Sbjct: 134 SVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 193

Query: 198 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF 257
           GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF
Sbjct: 194 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF 253

Query: 258 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLM 317
           RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI GWVKFWLM
Sbjct: 254 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLM 313

Query: 318 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 377
           PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI
Sbjct: 314 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 373

Query: 378 PHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDRL 437
           PHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENY+AFDRL
Sbjct: 374 PHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVAFDRL 433

Query: 438 NQEDSYPITFLRKVMPDLA 457
           N+EDSYPITFL+KVMPDLA
Sbjct: 434 NKEDSYPITFLKKVMPDLA 443

BLAST of Clc06G13300 vs. NCBI nr
Match: KAA0035068.1 (omega-6 fatty acid desaturase [Cucumis melo var. makuwa])

HSP 1 Score: 862.4 bits (2227), Expect = 1.7e-246
Identity = 419/492 (85.16%), Postives = 435/492 (88.41%), Query Frame = 0

Query: 1   MSDDNATLHTSGVSSKRGLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIK 60
           MSD+NA L+TSG SSK G HQN ARTQKIASH S G++ +          GEILFQKKIK
Sbjct: 1   MSDNNAALNTSGASSKGGPHQNLARTQKIASHCSPGTLQV---------KGEILFQKKIK 60

Query: 61  RQNYPLPMKRAAILRAVAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIV 120
           RQNY LP+K+AAILRAVAVPVAPP+SSADSAEYRKQLSESYGF+QIGEPLP+NVRLKDIV
Sbjct: 61  RQNYLLPVKKAAILRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIV 120

Query: 121 ESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITG------ 180
           ESLPKEVFEINDVKAWKSVLVSVASY+LGLVMIAKAPWYLLPLAWAWTGTAITG      
Sbjct: 121 ESLPKEVFEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGVSLIQI 180

Query: 181 ------------------------------FFVIGHDCAHKSFSRNKLVEDIVGTLAFMP 240
                                         FFVIGHDCAHKSFSRNKLVEDIVGTLAFMP
Sbjct: 181 FFLVASLSLSLLLILVRLAALATEVSFLPKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMP 240

Query: 241 LIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIA 300
           LIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYGYGPFRPWMSIA
Sbjct: 241 LIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYGYGPFRPWMSIA 300

Query: 301 HWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHF 360
           HWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI GWVKFWLMPWLGYHF
Sbjct: 301 HWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHF 360

Query: 361 WMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPR 420
           WMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPR
Sbjct: 361 WMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPR 420

Query: 421 IPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDRLNQEDSYP 457
           IPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVYSKEENY+AFDRLN+EDSYP
Sbjct: 421 IPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVAFDRLNKEDSYP 480

BLAST of Clc06G13300 vs. NCBI nr
Match: XP_004146685.1 (omega-6 fatty acid desaturase, chloroplastic isoform X1 [Cucumis sativus] >KGN65155.1 hypothetical protein Csa_019883 [Cucumis sativus])

HSP 1 Score: 860.9 bits (2223), Expect = 4.9e-246
Identity = 405/439 (92.26%), Postives = 419/439 (95.44%), Query Frame = 0

Query: 18  GLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMKRAAILRAV 77
           G H N ARTQKIASH S G + +          GEILFQKKIKRQNY LP+KRAAILRA+
Sbjct: 14  GPHHNFARTQKIASHCSPGILQV---------KGEILFQKKIKRQNYLLPVKRAAILRAI 73

Query: 78  AVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAWK 137
           AVP APP+SSADSAEYRKQLSESYGF+QIGEPLP+NVRLKD+VESLPKEVFEINDVKAWK
Sbjct: 74  AVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDVKAWK 133

Query: 138 SVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 197
           SVLVSVASY+LGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV
Sbjct: 134 SVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 193

Query: 198 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF 257
           GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF
Sbjct: 194 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF 253

Query: 258 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLM 317
           RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI GWVKFWLM
Sbjct: 254 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLM 313

Query: 318 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 377
           PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI
Sbjct: 314 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 373

Query: 378 PHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDRL 437
           PHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENY+AFDRL
Sbjct: 374 PHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVAFDRL 433

Query: 438 NQEDSYPITFLRKVMPDLA 457
           N+EDSYPITFL+KVMPDLA
Sbjct: 434 NKEDSYPITFLKKVMPDLA 443

BLAST of Clc06G13300 vs. ExPASy Swiss-Prot
Match: P48627 (Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 1.9e-200
Identity = 318/397 (80.10%), Postives = 358/397 (90.18%), Query Frame = 0

Query: 61  RQNYPLP-MKRAAILRAVAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDI 120
           R+ + +P  KR   ++AV VPVAPP  SAD+AE R+QL+ESYGFKQIG+ LP+NV LKDI
Sbjct: 48  RRTFLVPAKKRIGCIKAVFVPVAPP--SADNAEDREQLAESYGFKQIGQDLPDNVTLKDI 107

Query: 121 VESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIG 180
           +++LPKEVFEI+DVKAWKSVL+SV SY LGL MIAKAPWYLLPLAWAWTGTA+TGFFVIG
Sbjct: 108 MDTLPKEVFEIDDVKAWKSVLISVTSYALGLFMIAKAPWYLLPLAWAWTGTAVTGFFVIG 167

Query: 181 HDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEE 240
           HDCAHKSFS+NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EE
Sbjct: 168 HDCAHKSFSKNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEE 227

Query: 241 FDSAPLLRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGW 300
           FDS+P+LRKAII+GYGP RPW+SIAHW+ WHF+L+KFRP+EV RVKISLACVFAFM +GW
Sbjct: 228 FDSSPVLRKAIIFGYGPIRPWLSIAHWVNWHFNLRKFRPSEVNRVKISLACVFAFMAVGW 287

Query: 301 PLIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVH 360
           PLIIYK G+ GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVH
Sbjct: 288 PLIIYKVGVLGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVH 347

Query: 361 CAYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIM 420
           C YP WIEILCHDINVHIPHH+SPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIM
Sbjct: 348 CDYPSWIEILCHDINVHIPHHISPRIPSYNLRAAHQSIQENWGKYTNLATWNWRLMKTIM 407

Query: 421 TICHVYSKEENYIAFDRLNQEDSYPITFLRKVMPDLA 457
           T+CHVY KEENYI FDRL  E+S PITFL+K MPD A
Sbjct: 408 TVCHVYDKEENYIPFDRLAPEESQPITFLKKAMPDYA 442

BLAST of Clc06G13300 vs. ExPASy Swiss-Prot
Match: P46312 (Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD6 PE=1 SV=2)

HSP 1 Score: 688.0 bits (1774), Expect = 7.5e-197
Identity = 323/438 (73.74%), Postives = 372/438 (84.93%), Query Frame = 0

Query: 18  GLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMK-RAAILRA 77
           G  Q   R  K+A    + S  ++  + AV  +  +L  +  + +    P+K R   ++A
Sbjct: 14  GPQQCLPRVPKLA----ASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPVKRRIGCIKA 73

Query: 78  VAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAW 137
           VA PVAPP  SADSAE R+QL+ESYGF+QIGE LPENV LKDI+++LPKEVFEI+D+KA 
Sbjct: 74  VAAPVAPP--SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVFEIDDLKAL 133

Query: 138 KSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDI 197
           KSVL+SV SYTLGL MIAK+PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLVEDI
Sbjct: 134 KSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVEDI 193

Query: 198 VGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGP 257
           VGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S+P++RKAII+GYGP
Sbjct: 194 VGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMRKAIIFGYGP 253

Query: 258 FRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWL 317
            RPW+SIAHW+ WHF+LKKFR +EV RVKISLACVFAFM +GWPLI+YK GI GWVKFWL
Sbjct: 254 IRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVGILGWVKFWL 313

Query: 318 MPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVH 377
           MPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEILCHDINVH
Sbjct: 314 MPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEILCHDINVH 373

Query: 378 IPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDR 437
           IPHH+SPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY KEENYI FDR
Sbjct: 374 IPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEENYIPFDR 433

Query: 438 LNQEDSYPITFLRKVMPD 455
           L  E+S PITFL+K MP+
Sbjct: 434 LAPEESQPITFLKKAMPN 445

BLAST of Clc06G13300 vs. ExPASy Swiss-Prot
Match: P48628 (Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 5.4e-195
Identity = 314/420 (74.76%), Postives = 353/420 (84.05%), Query Frame = 0

Query: 17  RGLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMKRAAILRA 76
           +G +Q     + IA+ YS G   L             L QK+ +RQ   +   +  ++ A
Sbjct: 13  KGSYQKPVLRRDIAARYSPGIFSLN---------SNGLIQKRFRRQRNFVTRNKVTVIHA 72

Query: 77  VAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAW 136
           VA+PV P  +  +SAEYRKQL+E YGF+Q+GEPL ++V LKD++  LPKEVFEI+DVKAW
Sbjct: 73  VAIPVQP--APVESAEYRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDVKAW 132

Query: 137 KSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDI 196
           KSVL+SV SY LGL MI+KAPWYLLPLAW WTGTAITGFFVIGHDCAH+SFS NKLVEDI
Sbjct: 133 KSVLISVTSYALGLFMISKAPWYLLPLAWVWTGTAITGFFVIGHDCAHRSFSSNKLVEDI 192

Query: 197 VGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGP 256
           VGTLAFMPLIYPYEPWRFKHD+HHAKTNML EDTAWHPVWK+EF+S PLLRKAIIYGYGP
Sbjct: 193 VGTLAFMPLIYPYEPWRFKHDRHHAKTNMLREDTAWHPVWKDEFESTPLLRKAIIYGYGP 252

Query: 257 FRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWL 316
           FR WMSIAHWL+WHFDLKKFRP+EV RVKISLACVFAF+ IGWPLIIYKTGI GW+KFWL
Sbjct: 253 FRCWMSIAHWLMWHFDLKKFRPSEVPRVKISLACVFAFIAIGWPLIIYKTGIMGWIKFWL 312

Query: 317 MPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVH 376
           MPWLGYHFWMSTFTMVHHTAP+IPFK SEEWN AQAQLNGTVHC YP+WIEILCHDINVH
Sbjct: 313 MPWLGYHFWMSTFTMVHHTAPYIPFKYSEEWNRAQAQLNGTVHCDYPKWIEILCHDINVH 372

Query: 377 IPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDR 436
           IPHH+SPRIPSYNLRAAHKSLQENWG++LNEASWNWRLMKTIMT+C VY KE++     R
Sbjct: 373 IPHHISPRIPSYNLRAAHKSLQENWGQYLNEASWNWRLMKTIMTVCQVYDKEKSLCCLRR 421

BLAST of Clc06G13300 vs. ExPASy Swiss-Prot
Match: P48629 (Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD6 PE=1 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 4.0e-182
Identity = 295/446 (66.14%), Postives = 363/446 (81.39%), Query Frame = 0

Query: 13  VSSKRGLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMKRAA 72
           +S+   L  + +R   +++  S+G   +      + ++G +   ++ K        +++ 
Sbjct: 7   ISNHVNLAFSLSRNPSLSTKNSAGISCIKWQRPCLRNLGHVRLNQQRKGTR-----RKST 66

Query: 73  ILRAVAVPVAPPTS--SADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEI 132
           +++AVAVPVA P++    D+ E+ KQL+E YGF+QIGEPLP++V ++DI+ SLPK+VFEI
Sbjct: 67  LVQAVAVPVAQPSAFPPTDNTEHLKQLAERYGFQQIGEPLPDDVTMRDIITSLPKQVFEI 126

Query: 133 NDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRN 192
           ND KAW +VL+SV SY LG+ MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+N
Sbjct: 127 NDTKAWGTVLISVTSYALGIFMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKN 186

Query: 193 KLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAI 252
           KLVEDIVGTLAFMPLIYPYEPWRFKHDQHH KTNML EDTAW P+ KE+ +S+P LRKA+
Sbjct: 187 KLVEDIVGTLAFMPLIYPYEPWRFKHDQHHTKTNMLREDTAWLPIMKEDIESSPGLRKAL 246

Query: 253 IYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFG 312
           IY YGP R WMSIAHWL  HF+LK FR +EVKR  ISLA VFAFM IGWPLIIYKTGI G
Sbjct: 247 IYAYGPLRTWMSIAHWLKVHFNLKDFRQSEVKRATISLAAVFAFMVIGWPLIIYKTGIVG 306

Query: 313 WVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILC 372
           W+KFWLMPWLGYHFWMSTFT+VHHTAPHIPFKSS+EWNAAQAQL+GTVHC YP+WIEILC
Sbjct: 307 WIKFWLMPWLGYHFWMSTFTIVHHTAPHIPFKSSKEWNAAQAQLSGTVHCDYPRWIEILC 366

Query: 373 HDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEEN 432
           HDI+VHIPHH+SP+IPSYNLRAA++SL ENWG++LN+   NWRLM+TIMT CH+Y K+ N
Sbjct: 367 HDISVHIPHHISPKIPSYNLRAANQSLNENWGEYLNKPKSNWRLMRTIMTTCHIYDKDGN 426

Query: 433 YIAFDRLNQEDSYPITFLRKVMPDLA 457
           Y++F++   E+S PI+  ++VMPD A
Sbjct: 427 YVSFEKAVPEESQPISIPKRVMPDYA 447

BLAST of Clc06G13300 vs. ExPASy Swiss-Prot
Match: P20388 (Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=desA PE=1 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 3.2e-107
Identity = 174/328 (53.05%), Postives = 228/328 (69.51%), Query Frame = 0

Query: 113 NVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAI 172
           +++L+DI+++LPKE FE    KAW SVL+++ +  +G + I   PWY LP+ W WTGTA+
Sbjct: 23  DLKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGYLGIIYLPWYCLPITWIWTGTAL 82

Query: 173 TGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAW 232
           TG FV+GHDC H+SF++ + V D+VG +AF PLIYP+  WR  HD HH  TN +E D AW
Sbjct: 83  TGAFVVGHDCGHRSFAKKRWVNDLVGHIAFAPLIYPFHSWRLLHDHHHLHTNKIEVDNAW 142

Query: 233 HPVWKEEFDSAPLLRKAIIYGY-GPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACV 292
            P   E F ++P + +       GPF    SI HW L HF L  F   +  +VK+S+A V
Sbjct: 143 DPWSVEAFQASPAIVRLFYRAIRGPFWWTGSIFHWSLMHFKLSNFAQRDRNKVKLSIAVV 202

Query: 293 FAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQ 352
           F F  I +P +I  TG++G+VKFWLMPWL YHFWMSTFT+VHHT P I F+ + +W+AA+
Sbjct: 203 FLFAAIAFPALIITTGVWGFVKFWLMPWLVYHFWMSTFTIVHHTIPEIRFRPAADWSAAE 262

Query: 353 AQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWN 412
           AQLNGTVHC YP+W+E+LCHDINVHIPHH+S  IPSYNLR AH SL+ENWG  L E ++N
Sbjct: 263 AQLNGTVHCDYPRWVEVLCHDINVHIPHHLSVAIPSYNLRLAHGSLKENWGPFLYERTFN 322

Query: 413 WRLMKTIMTICHVYSKEENYIAFDRLNQ 440
           W+LM+ I   CH+Y  E  Y  F  L +
Sbjct: 323 WQLMQQISGQCHLYDPEHGYRTFGSLKK 350

BLAST of Clc06G13300 vs. ExPASy TrEMBL
Match: A0A5D3BV55 (Omega-6 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00370 PE=4 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 2.9e-252
Identity = 419/456 (91.89%), Postives = 435/456 (95.39%), Query Frame = 0

Query: 1   MSDDNATLHTSGVSSKRGLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIK 60
           MSD+NA L+TSG SSK G HQN ARTQKIASH S G++ +          GEILFQKKIK
Sbjct: 1   MSDNNAALNTSGASSKGGPHQNLARTQKIASHCSPGTLQV---------KGEILFQKKIK 60

Query: 61  RQNYPLPMKRAAILRAVAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIV 120
           RQNY LP+K+AAILRAVAVPVAPP+SSADSAEYRKQLSESYGF+QIGEPLP+NVRLKDIV
Sbjct: 61  RQNYLLPVKKAAILRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIV 120

Query: 121 ESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGH 180
           ESLPKEVFEINDVKAWKSVLVSVASY+LGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGH
Sbjct: 121 ESLPKEVFEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGH 180

Query: 181 DCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEF 240
           DCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEF
Sbjct: 181 DCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEF 240

Query: 241 DSAPLLRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWP 300
           DSAP+LRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWP
Sbjct: 241 DSAPILRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWP 300

Query: 301 LIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHC 360
           LIIYKTGI GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHC
Sbjct: 301 LIIYKTGILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHC 360

Query: 361 AYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT 420
           AYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT
Sbjct: 361 AYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT 420

Query: 421 ICHVYSKEENYIAFDRLNQEDSYPITFLRKVMPDLA 457
           +CHVYSKEENY+AFDRLN+EDSYPITFL+KVMPDLA
Sbjct: 421 VCHVYSKEENYVAFDRLNKEDSYPITFLKKVMPDLA 447

BLAST of Clc06G13300 vs. ExPASy TrEMBL
Match: A0A5A7SWT4 (Omega-6 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G001250 PE=4 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.2e-247
Identity = 419/492 (85.16%), Postives = 435/492 (88.41%), Query Frame = 0

Query: 1   MSDDNATLHTSGVSSKRGLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIK 60
           MSD+NA L+TSG SSK G HQN ARTQKIASH S G++ +          GEILFQKKIK
Sbjct: 1   MSDNNAALNTSGASSKGGPHQNLARTQKIASHCSPGTLQV---------KGEILFQKKIK 60

Query: 61  RQNYPLPMKRAAILRAVAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIV 120
           RQNY LP+K+AAILRAVAVPVAPP+SSADSAEYRKQLSESYGF+QIGEPLP+NVRLKDIV
Sbjct: 61  RQNYLLPVKKAAILRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIV 120

Query: 121 ESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITG------ 180
           ESLPKEVFEINDVKAWKSVLVSVASY+LGLVMIAKAPWYLLPLAWAWTGTAITG      
Sbjct: 121 ESLPKEVFEINDVKAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGVSLIQI 180

Query: 181 ------------------------------FFVIGHDCAHKSFSRNKLVEDIVGTLAFMP 240
                                         FFVIGHDCAHKSFSRNKLVEDIVGTLAFMP
Sbjct: 181 FFLVASLSLSLLLILVRLAALATEVSFLPKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMP 240

Query: 241 LIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIA 300
           LIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYGYGPFRPWMSIA
Sbjct: 241 LIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYGYGPFRPWMSIA 300

Query: 301 HWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHF 360
           HWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI GWVKFWLMPWLGYHF
Sbjct: 301 HWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHF 360

Query: 361 WMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPR 420
           WMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPR
Sbjct: 361 WMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPR 420

Query: 421 IPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDRLNQEDSYP 457
           IPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVYSKEENY+AFDRLN+EDSYP
Sbjct: 421 IPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVAFDRLNKEDSYP 480

BLAST of Clc06G13300 vs. ExPASy TrEMBL
Match: A0A0A0LTB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G255170 PE=4 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.4e-246
Identity = 405/439 (92.26%), Postives = 419/439 (95.44%), Query Frame = 0

Query: 18  GLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMKRAAILRAV 77
           G H N ARTQKIASH S G + +          GEILFQKKIKRQNY LP+KRAAILRA+
Sbjct: 14  GPHHNFARTQKIASHCSPGILQV---------KGEILFQKKIKRQNYLLPVKRAAILRAI 73

Query: 78  AVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAWK 137
           AVP APP+SSADSAEYRKQLSESYGF+QIGEPLP+NVRLKD+VESLPKEVFEINDVKAWK
Sbjct: 74  AVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDVKAWK 133

Query: 138 SVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 197
           SVLVSVASY+LGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV
Sbjct: 134 SVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 193

Query: 198 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF 257
           GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF
Sbjct: 194 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF 253

Query: 258 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLM 317
           RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI GWVKFWLM
Sbjct: 254 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLM 313

Query: 318 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 377
           PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI
Sbjct: 314 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 373

Query: 378 PHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDRL 437
           PHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENY+AFDRL
Sbjct: 374 PHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVAFDRL 433

Query: 438 NQEDSYPITFLRKVMPDLA 457
           N+EDSYPITFL+KVMPDLA
Sbjct: 434 NKEDSYPITFLKKVMPDLA 443

BLAST of Clc06G13300 vs. ExPASy TrEMBL
Match: A0A1S3B9S5 (omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487358 PE=4 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.1e-246
Identity = 407/439 (92.71%), Postives = 421/439 (95.90%), Query Frame = 0

Query: 18  GLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMKRAAILRAV 77
           G HQN ARTQKIASH S G++ +          GEILFQKKIKRQNY LP+K+AAILRAV
Sbjct: 14  GPHQNLARTQKIASHCSPGTLQV---------KGEILFQKKIKRQNYLLPVKKAAILRAV 73

Query: 78  AVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAWK 137
           AVPVAPP+SSADSAEYRKQLSESYGF+QIGEPLP+NVRLKDIVESLPKEVFEINDVKAWK
Sbjct: 74  AVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDVKAWK 133

Query: 138 SVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 197
           SVLVSVASY+LGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV
Sbjct: 134 SVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 193

Query: 198 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF 257
           GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYGYGPF
Sbjct: 194 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYGYGPF 253

Query: 258 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLM 317
           RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI GWVKFWLM
Sbjct: 254 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLM 313

Query: 318 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 377
           PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI
Sbjct: 314 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 373

Query: 378 PHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDRL 437
           PHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVYSKEENY+AFDRL
Sbjct: 374 PHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVAFDRL 433

Query: 438 NQEDSYPITFLRKVMPDLA 457
           N+EDSYPITFL+KVMPDLA
Sbjct: 434 NKEDSYPITFLKKVMPDLA 443

BLAST of Clc06G13300 vs. ExPASy TrEMBL
Match: A0A6J1EQE5 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436860 PE=4 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.5e-245
Identity = 402/439 (91.57%), Postives = 418/439 (95.22%), Query Frame = 0

Query: 18  GLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMKRAAILRAV 77
           G HQN ARTQKIASH + G + +          G+I+FQKKIKRQNY LP+KRAAILRAV
Sbjct: 14  GPHQNYARTQKIASHNAPGILDV---------KGKIVFQKKIKRQNYLLPVKRAAILRAV 73

Query: 78  AVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAWK 137
           AVPVAPP+SSAD AEYRKQLSESYGF+QIGEPLPENVRLKDI+ESLPKEVFEINDVKAWK
Sbjct: 74  AVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDVKAWK 133

Query: 138 SVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 197
           SVLVSVASYTLGL+MIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV
Sbjct: 134 SVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIV 193

Query: 198 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPF 257
           GTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YGYGPF
Sbjct: 194 GTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYGYGPF 253

Query: 258 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLM 317
           RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI GWVKFWLM
Sbjct: 254 RPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLM 313

Query: 318 PWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 377
           PWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI
Sbjct: 314 PWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHI 373

Query: 378 PHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDRL 437
           PHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENY+AFDRL
Sbjct: 374 PHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVAFDRL 433

Query: 438 NQEDSYPITFLRKVMPDLA 457
             EDSYPITFL+KVMPDLA
Sbjct: 434 KPEDSYPITFLKKVMPDLA 443

BLAST of Clc06G13300 vs. TAIR 10
Match: AT4G30950.1 (fatty acid desaturase 6 )

HSP 1 Score: 688.0 bits (1774), Expect = 5.3e-198
Identity = 323/438 (73.74%), Postives = 372/438 (84.93%), Query Frame = 0

Query: 18  GLHQNSARTQKIASHYSSGSIPLTLSLLAVADMGEILFQKKIKRQNYPLPMK-RAAILRA 77
           G  Q   R  K+A    + S  ++  + AV  +  +L  +  + +    P+K R   ++A
Sbjct: 14  GPQQCLPRVPKLA----ASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPVKRRIGCIKA 73

Query: 78  VAVPVAPPTSSADSAEYRKQLSESYGFKQIGEPLPENVRLKDIVESLPKEVFEINDVKAW 137
           VA PVAPP  SADSAE R+QL+ESYGF+QIGE LPENV LKDI+++LPKEVFEI+D+KA 
Sbjct: 74  VAAPVAPP--SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVFEIDDLKAL 133

Query: 138 KSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDI 197
           KSVL+SV SYTLGL MIAK+PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFS+NKLVEDI
Sbjct: 134 KSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSKNKLVEDI 193

Query: 198 VGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGP 257
           VGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S+P++RKAII+GYGP
Sbjct: 194 VGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMRKAIIFGYGP 253

Query: 258 FRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWL 317
            RPW+SIAHW+ WHF+LKKFR +EV RVKISLACVFAFM +GWPLI+YK GI GWVKFWL
Sbjct: 254 IRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVGILGWVKFWL 313

Query: 318 MPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVH 377
           MPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEILCHDINVH
Sbjct: 314 MPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEILCHDINVH 373

Query: 378 IPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIAFDR 437
           IPHH+SPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY KEENYI FDR
Sbjct: 374 IPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEENYIPFDR 433

Query: 438 LNQEDSYPITFLRKVMPD 455
           L  E+S PITFL+K MP+
Sbjct: 434 LAPEESQPITFLKKAMPN 445

BLAST of Clc06G13300 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-29
Identity = 97/344 (28.20%), Postives = 153/344 (44.48%), Query Frame = 0

Query: 107 GEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKAP----WYLLP 166
           G P P N  L DI  ++PK  +  N    WKS+   V    +   + A A     W + P
Sbjct: 91  GAPPPFN--LADIRAAIPKHCWVKN---PWKSLSYVVRDVAIVFALAAGAAYLNNWIVWP 150

Query: 167 LAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAK 226
           L W   GT     FV+GHDC H SFS +  +  +VG L    ++ PY  WR  H  HH  
Sbjct: 151 LYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQN 210

Query: 227 TNMLEEDTAWHPVWKEEFD-----------SAPLLRKAIIYGYGPFRPWMSIAHWLLWHF 286
              +E D +WHP+ ++ ++           + PL+  A  +      P    +H   +H 
Sbjct: 211 HGHVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSH---YHP 270

Query: 287 DLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHFWMSTFTM 346
           D   F P E K V  S AC  A   +    + +  G    +K + +P+     W+   T 
Sbjct: 271 DSDLFLPKERKDVLTSTACWTAMAAL-LVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTY 330

Query: 347 VHHTA--PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIPSYN 406
           +HH      +P+   +EW+  +  L  T+   Y   I  + HDI  H+ HH+ P+IP Y+
Sbjct: 331 LHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GLINNIHHDIGTHVIHHLFPQIPHYH 390

Query: 407 LRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYIA 434
           L  A ++ +   GK+  E   +  L   ++ I     KE++Y++
Sbjct: 391 LVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS 423

BLAST of Clc06G13300 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 125.9 bits (315), Expect = 8.1e-29
Identity = 85/334 (25.45%), Postives = 158/334 (47.31%), Query Frame = 0

Query: 115 RLKDIVESLPKEVFEINDVKAWKSV---LVSVASYTLGLVMIAKAPWYLLPLAWAWTGTA 174
           ++ DI  ++PK  +  + +++   V   +++VA+  +  V +    W+L PL WA  GT 
Sbjct: 35  KIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAALAIAAVYVDS--WFLWPLYWAAQGTL 94

Query: 175 ITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTA 234
               FV+GHDC H SFS   L+  +VG +    ++ PY  WR  H  HH     +E D +
Sbjct: 95  FWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQNHGHVENDES 154

Query: 235 WHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHWLLW--------HFD--LKKFRPNEV 294
           W P+ +  +   P   + + Y      P ++   +L +        HF+     F P+E 
Sbjct: 155 WVPLPERVYKKLPHSTRMLRYTVP--LPMLAYPLYLCYRSPGKEGSHFNPYSSLFAPSER 214

Query: 295 KRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTA--PHI 354
           K +  S  C ++ MF+    + +  G    +K + +P++ +  W+   T +HH      +
Sbjct: 215 KLIATSTTC-WSIMFVSLIALSFVFGPLAVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKL 274

Query: 355 PFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAHKSLQE 414
           P+   +EW+  +  L  T+   Y  +  I  HDI  H+ HH+ P+IP Y+L  A K+ + 
Sbjct: 275 PWYRGKEWSYLRGGLT-TIDRDYGIFNNI-HHDIGTHVIHHLFPQIPHYHLVDATKAAKH 334

Query: 415 NWGKHLNEASWNWRLMKTIMTICHVYSKEENYIA 434
             G++  E   +  +   ++       K+++Y++
Sbjct: 335 VLGRYYREPKTSGAIPIHLVESLVASIKKDHYVS 361

BLAST of Clc06G13300 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 123.6 bits (309), Expect = 4.0e-28
Identity = 93/313 (29.71%), Postives = 139/313 (44.41%), Query Frame = 0

Query: 107 GEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAK--APWYLLPLA 166
           G P P N  L DI  ++PK  +  N   +   V+  VA    GL  +A     W L PL 
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLWPLY 143

Query: 167 WAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTN 226
           W   GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH    
Sbjct: 144 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 203

Query: 227 MLEEDTAWHP----VWKEEFDSAPLLRKAIIYGY--GPFRPWMSI--AHWLLWHFDLKKF 286
            +E D +WHP    ++K    +  + R  + +     PF  W          +H D   F
Sbjct: 204 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 263

Query: 287 RPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTA 346
            P E K V  S AC  A   +    + +  G    +K + +P+  +  W+   T +HH  
Sbjct: 264 LPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHG 323

Query: 347 --PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIPSYNLRAAH 406
               +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L  A 
Sbjct: 324 HEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHLVEAT 383

Query: 407 KSLQENWGKHLNE 408
           ++ +   GK+  E
Sbjct: 384 EAAKPVLGKYYRE 390

BLAST of Clc06G13300 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 119.4 bits (298), Expect = 7.5e-27
Identity = 89/296 (30.07%), Postives = 131/296 (44.26%), Query Frame = 0

Query: 107 GEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAK--APWYLLPLA 166
           G P P N  L DI  ++PK  +  N   +   V+  VA    GL  +A     W L PL 
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLWPLY 143

Query: 167 WAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTN 226
           W   GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH    
Sbjct: 144 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 203

Query: 227 MLEEDTAWHP----VWKEEFDSAPLLRKAIIYGY--GPFRPWMSI--AHWLLWHFDLKKF 286
            +E D +WHP    ++K    +  + R  + +     PF  W          +H D   F
Sbjct: 204 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 263

Query: 287 RPNEVKRVKISLACVFAFMFIGWPLIIYKTGIFGWVKFWLMPWLGYHFWMSTFTMVHHTA 346
            P E K V  S AC  A   +    + +  G    +K + +P+  +  W+   T +HH  
Sbjct: 264 LPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHG 323

Query: 347 --PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIPSYNL 391
               +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L
Sbjct: 324 HEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHL 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK03611.16.0e-25291.89omega-6 fatty acid desaturase [Cucumis melo var. makuwa][more]
KAG6587828.13.1e-24887.74hypothetical protein SDJN03_16393, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038880078.11.2e-24793.39omega-6 fatty acid desaturase, chloroplastic [Benincasa hispida][more]
KAA0035068.11.7e-24685.16omega-6 fatty acid desaturase [Cucumis melo var. makuwa][more]
XP_004146685.14.9e-24692.26omega-6 fatty acid desaturase, chloroplastic isoform X1 [Cucumis sativus] >KGN65... [more]
Match NameE-valueIdentityDescription
P486271.9e-20080.10Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1[more]
P463127.5e-19773.74Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P486285.4e-19574.76Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1[more]
P486294.0e-18266.14Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD... [more]
P203883.2e-10753.05Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ... [more]
Match NameE-valueIdentityDescription
A0A5D3BV552.9e-25291.89Omega-6 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A5A7SWT48.2e-24785.16Omega-6 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A0A0LTB12.4e-24692.26FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G2551... [more]
A0A1S3B9S53.1e-24692.71omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1EQE54.5e-24591.57omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT4G30950.15.3e-19873.74fatty acid desaturase 6 [more]
AT3G11170.11.6e-2928.20fatty acid desaturase 7 [more]
AT2G29980.18.1e-2925.45fatty acid desaturase 3 [more]
AT5G05580.14.0e-2829.71fatty acid desaturase 8 [more]
AT5G05580.27.5e-2730.07fatty acid desaturase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 158..405
e-value: 4.3E-30
score: 105.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 73..456
NoneNo IPR availablePANTHERPTHR32100:SF67BNAC03G67820D PROTEINcoord: 73..456
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 134..390
e-value: 1.74557E-81
score: 249.065

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc06G13300.2Clc06G13300.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane