Clc06G12270 (gene) Watermelon (cordophanus) v2

Overview
NameClc06G12270
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionENTH domain-containing protein
LocationClcChr06: 22950262 .. 22952436 (-)
RNA-Seq ExpressionClc06G12270
SyntenyClc06G12270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAGAATGCACAAGTACAAGTCAACACCATGCCGTTTTCTTCTCTCTCAAATGCTCTTTCGCTTTCAGCGTTTCTGAAGAAAGCGCTTTTCCTTCTCTGCTTCTTCATCATCGCCACTACTCCACATTTACTAATTTTCATTTCACTTCACTATCTACACGCCAACTGAAGTTTTGTTCGTAATTTTGTACGAAAATGGCTCAAACCACCATCAGAAAAGCCATCGGAGCTATGAAGGACCAAACGACCATCAGCATCGCCAAAGTCGCCGGAAATATGTCGCCGGAGCTCGATGTCTTGGTGGTCAAAGCGACAAGCCACGATGAGAATCCCGCCGACGATAGGTATATTAGGGAGATTGTGAATTTATGCTCGAACTGTTCGAATGGATATGTGAGTGCGTGTGTAGTTACGATCTCTAAACGATGGAGTAAAACTCGGGACTGGATCGTTGCGCTCAAGGCACTAATTGTGGTACATAGGGTTTTAATTGATGGACATCCCTCTTTTGGGGAGGAGATTGTGTATGCTACGCGAAAAGGTATGCGAGTCTTGGATTTATCTGGTTTTAGAGATGAAGCACATTCGAATTCATGGGATCATTCTAGTTTTTTGAGGTTTTACGCTTTGTATCTTGATGAATTGATTGAGAACCTGGTTCTTGAGAAGAGAGTGAAAGGGGTTGACAAATCTTATTATAGGAGCAGGAGGCAGAGTCCATGGAGGTCAATGAGACCAAAGAGAGTAGTAGAGGAGTTAAACCAATTGCTTCAATTGCTTGATCGAGTTTTAGCTTGTAGGCCGGCAGGAATGGCTAAGAGTAGTAGATTAGTAATTACTGCGCTTAATTTGGTAATCAAAGAGAGCTTTGGGTTGTATATTGAGATTTGTGATGCATTGGGGGTTTTGTTGGATCACTTTAAAGAAATGGAATATGGTGATTGTCTTAGGGCTTTTGATATTTGTGCTAATGCTGCAAAGAATATTGATGAACTTGTGGAATTTTATGCTTGGTGTAAGGATGTGGGGATTGCAGGGTCTTCTGAATATCCAGAGGTTCAGATGATAACTCATAACATTTTGATTACACTTGGGGGCTTCCTAAAGGAGATGTCAAGAAGGCCTAAGGATTACAATGAAACGAGAATGGAGGAGGAGATGAAGCCGCTACCGGTTAAGGAGCTTGAATATGACATGAATGAGATAAAGACTCTTCCTCCACTAGAAAATTGTGTTCCTGTTATGCAATCAGTCTTGCGAACTCAATCGCGAAAGGAAGACTTGGTGAACTTGAGAGAGGATGGAGTATCAGCAGATGAGCAAGGGAACAAATTGGCCTTGGCTCTTTTCTCTGGACCCCCAATTACAAATCCTAATGGTTCATGGGAAACTTTCTCATCAAACCACGAGCCTGACGCGAGCTCAGTGTGGCAGACGCCAGTTGCTGAGTTTGGCAAACCAGATTGGGAATTGGCATTGGTTGAAACTGCTAGCCATCTATCAAAACAGAAGCCTGATATGGGAGGTTGTTTTCATCCACTGCTATTAAATGGAATGTACGACCAAGAGATGGTTAGGCGACACGTCAATGTCGAGCAATTCGGGGGGAGTTCGAGTAGTGTGGCAGTGGCATTGCAAGGCCAAGGCAATGCTGCTGCACTTCTGGCACTTCCAGCACCAGATGGAATGGTTGAACCGATCAGGTATCGGGACCCTTTCGCTGCATCGCTTACGGTGCCACCTCCTTCGTATGTGCAGATAGCTGAAATGGAGAAGAAGCAGCAGTTGCTAATGCAGGAACAGCTTCTATGGAAGCAGTATGGCAGAGATGGAATGCAGGGCCAAGTGGGTTTGGGAAAACTTGGTGGTGGCTCGGGTTATGTTGGCTTAAATCCTCAGCAAATCATGCCTCATCGCTCAGCCTACTACTATGCTCCGTTTTGAATTGGCTTTCATTTTGAGCCTAAGCTCCATATAGTTACTACAAGGACCAATAACAGAGAAAAGTCGATTGTATATATTTTAATATTTTCAACAGGTTGTCCTTATGTTTTCCGTCTTTTTATTTGCTTGCTTACATCAATGGAGTAAATAAAAGGCCTAAGGTCTTCCGATAACAATTTGGTTTTGTATCGTTCGTGACAGTTTCCAGTTTCCAGTTTTTTGTTTTTCAT

mRNA sequence

TAAAGAATGCACAAGTACAAGTCAACACCATGCCGTTTTCTTCTCTCTCAAATGCTCTTTCGCTTTCAGCGTTTCTGAAGAAAGCGCTTTTCCTTCTCTGCTTCTTCATCATCGCCACTACTCCACATTTACTAATTTTCATTTCACTTCACTATCTACACGCCAACTGAAGTTTTGTTCGTAATTTTGTACGAAAATGGCTCAAACCACCATCAGAAAAGCCATCGGAGCTATGAAGGACCAAACGACCATCAGCATCGCCAAAGTCGCCGGAAATATGTCGCCGGAGCTCGATGTCTTGGTGGTCAAAGCGACAAGCCACGATGAGAATCCCGCCGACGATAGGTATATTAGGGAGATTGTGAATTTATGCTCGAACTGTTCGAATGGATATGTGAGTGCGTGTGTAGTTACGATCTCTAAACGATGGAGTAAAACTCGGGACTGGATCGTTGCGCTCAAGGCACTAATTGTGGTACATAGGGTTTTAATTGATGGACATCCCTCTTTTGGGGAGGAGATTGTGTATGCTACGCGAAAAGGTATGCGAGTCTTGGATTTATCTGGTTTTAGAGATGAAGCACATTCGAATTCATGGGATCATTCTAGTTTTTTGAGGTTTTACGCTTTGTATCTTGATGAATTGATTGAGAACCTGGTTCTTGAGAAGAGAGTGAAAGGGGTTGACAAATCTTATTATAGGAGCAGGAGGCAGAGTCCATGGAGGTCAATGAGACCAAAGAGAGTAGTAGAGGAGTTAAACCAATTGCTTCAATTGCTTGATCGAGTTTTAGCTTGTAGGCCGGCAGGAATGGCTAAGAGTAGTAGATTAGTAATTACTGCGCTTAATTTGGTAATCAAAGAGAGCTTTGGGTTGTATATTGAGATTTGTGATGCATTGGGGGTTTTGTTGGATCACTTTAAAGAAATGGAATATGGTGATTGTCTTAGGGCTTTTGATATTTGTGCTAATGCTGCAAAGAATATTGATGAACTTGTGGAATTTTATGCTTGGTGTAAGGATGTGGGGATTGCAGGGTCTTCTGAATATCCAGAGGTTCAGATGATAACTCATAACATTTTGATTACACTTGGGGGCTTCCTAAAGGAGATGTCAAGAAGGCCTAAGGATTACAATGAAACGAGAATGGAGGAGGAGATGAAGCCGCTACCGGTTAAGGAGCTTGAATATGACATGAATGAGATAAAGACTCTTCCTCCACTAGAAAATTGTGTTCCTGTTATGCAATCAGTCTTGCGAACTCAATCGCGAAAGGAAGACTTGGTGAACTTGAGAGAGGATGGAGTATCAGCAGATGAGCAAGGGAACAAATTGGCCTTGGCTCTTTTCTCTGGACCCCCAATTACAAATCCTAATGGTTCATGGGAAACTTTCTCATCAAACCACGAGCCTGACGCGAGCTCAGTGTGGCAGACGCCAGTTGCTGAGTTTGGCAAACCAGATTGGGAATTGGCATTGGTTGAAACTGCTAGCCATCTATCAAAACAGAAGCCTGATATGGGAGGTTGTTTTCATCCACTGCTATTAAATGGAATGTACGACCAAGAGATGGTTAGGCGACACGTCAATGTCGAGCAATTCGGGGGGAGTTCGAGTAGTGTGGCAGTGGCATTGCAAGGCCAAGGCAATGCTGCTGCACTTCTGGCACTTCCAGCACCAGATGGAATGGTTGAACCGATCAGGTATCGGGACCCTTTCGCTGCATCGCTTACGGTGCCACCTCCTTCGTATGTGCAGATAGCTGAAATGGAGAAGAAGCAGCAGTTGCTAATGCAGGAACAGCTTCTATGGAAGCAGTATGGCAGAGATGGAATGCAGGGCCAAGTGGGTTTGGGAAAACTTGGTGGTGGCTCGGGTTATGTTGGCTTAAATCCTCAGCAAATCATGCCTCATCGCTCAGCCTACTACTATGCTCCGTTTTGAATTGGCTTTCATTTTGAGCCTAAGCTCCATATAGTTACTACAAGGACCAATAACAGAGAAAAGTCGATTGTATATATTTTAATATTTTCAACAGGTTGTCCTTATGTTTTCCGTCTTTTTATTTGCTTGCTTACATCAATGGAGTAAATAAAAGGCCTAAGGTCTTCCGATAACAATTTGGTTTTGTATCGTTCGTGACAGTTTCCAGTTTCCAGTTTTTTGTTTTTCAT

Coding sequence (CDS)

ATGGCTCAAACCACCATCAGAAAAGCCATCGGAGCTATGAAGGACCAAACGACCATCAGCATCGCCAAAGTCGCCGGAAATATGTCGCCGGAGCTCGATGTCTTGGTGGTCAAAGCGACAAGCCACGATGAGAATCCCGCCGACGATAGGTATATTAGGGAGATTGTGAATTTATGCTCGAACTGTTCGAATGGATATGTGAGTGCGTGTGTAGTTACGATCTCTAAACGATGGAGTAAAACTCGGGACTGGATCGTTGCGCTCAAGGCACTAATTGTGGTACATAGGGTTTTAATTGATGGACATCCCTCTTTTGGGGAGGAGATTGTGTATGCTACGCGAAAAGGTATGCGAGTCTTGGATTTATCTGGTTTTAGAGATGAAGCACATTCGAATTCATGGGATCATTCTAGTTTTTTGAGGTTTTACGCTTTGTATCTTGATGAATTGATTGAGAACCTGGTTCTTGAGAAGAGAGTGAAAGGGGTTGACAAATCTTATTATAGGAGCAGGAGGCAGAGTCCATGGAGGTCAATGAGACCAAAGAGAGTAGTAGAGGAGTTAAACCAATTGCTTCAATTGCTTGATCGAGTTTTAGCTTGTAGGCCGGCAGGAATGGCTAAGAGTAGTAGATTAGTAATTACTGCGCTTAATTTGGTAATCAAAGAGAGCTTTGGGTTGTATATTGAGATTTGTGATGCATTGGGGGTTTTGTTGGATCACTTTAAAGAAATGGAATATGGTGATTGTCTTAGGGCTTTTGATATTTGTGCTAATGCTGCAAAGAATATTGATGAACTTGTGGAATTTTATGCTTGGTGTAAGGATGTGGGGATTGCAGGGTCTTCTGAATATCCAGAGGTTCAGATGATAACTCATAACATTTTGATTACACTTGGGGGCTTCCTAAAGGAGATGTCAAGAAGGCCTAAGGATTACAATGAAACGAGAATGGAGGAGGAGATGAAGCCGCTACCGGTTAAGGAGCTTGAATATGACATGAATGAGATAAAGACTCTTCCTCCACTAGAAAATTGTGTTCCTGTTATGCAATCAGTCTTGCGAACTCAATCGCGAAAGGAAGACTTGGTGAACTTGAGAGAGGATGGAGTATCAGCAGATGAGCAAGGGAACAAATTGGCCTTGGCTCTTTTCTCTGGACCCCCAATTACAAATCCTAATGGTTCATGGGAAACTTTCTCATCAAACCACGAGCCTGACGCGAGCTCAGTGTGGCAGACGCCAGTTGCTGAGTTTGGCAAACCAGATTGGGAATTGGCATTGGTTGAAACTGCTAGCCATCTATCAAAACAGAAGCCTGATATGGGAGGTTGTTTTCATCCACTGCTATTAAATGGAATGTACGACCAAGAGATGGTTAGGCGACACGTCAATGTCGAGCAATTCGGGGGGAGTTCGAGTAGTGTGGCAGTGGCATTGCAAGGCCAAGGCAATGCTGCTGCACTTCTGGCACTTCCAGCACCAGATGGAATGGTTGAACCGATCAGGTATCGGGACCCTTTCGCTGCATCGCTTACGGTGCCACCTCCTTCGTATGTGCAGATAGCTGAAATGGAGAAGAAGCAGCAGTTGCTAATGCAGGAACAGCTTCTATGGAAGCAGTATGGCAGAGATGGAATGCAGGGCCAAGTGGGTTTGGGAAAACTTGGTGGTGGCTCGGGTTATGTTGGCTTAAATCCTCAGCAAATCATGCCTCATCGCTCAGCCTACTACTATGCTCCGTTTTGA

Protein sequence

MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCSNCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVLDLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYYRSRRQSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVLRTQSRKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFGGSSSSVAVALQGQGNAAALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWKQYGRDGMQGQVGLGKLGGGSGYVGLNPQQIMPHRSAYYYAPF
Homology
BLAST of Clc06G12270 vs. NCBI nr
Match: XP_038879765.1 (putative clathrin assembly protein At2g25430 [Benincasa hispida])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.4e-300
Identity = 520/583 (89.19%), Postives = 547/583 (93.83%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKAIG MKDQTTISIAKVAGNM+PELDVLVVKATSHDE+PADDRYIREIVNLCS
Sbjct: 1   MAQSTIRKAIGVMKDQTTISIAKVAGNMTPELDVLVVKATSHDEDPADDRYIREIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
           N SNGYV+ACVVTIS+R SKTRDWIVALK+LIVVHR+LIDGHPSFGEEIVYATRKGMRVL
Sbjct: 61  NYSNGYVNACVVTISRRLSKTRDWIVALKSLIVVHRILIDGHPSFGEEIVYATRKGMRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYYRSRRQSPWRSMR 180
           +LSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKR+KG D+S YRSR+QSPWRSMR
Sbjct: 121 NLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRLKGGDESCYRSRKQSPWRSMR 180

Query: 181 PKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVLLD 240
           P+RVVEELNQLL++LDRVLACRPAGMAK+SRLVITALNL+IKESFGLY+EICDALGVLLD
Sbjct: 181 PERVVEELNQLLRMLDRVLACRPAGMAKNSRLVITALNLIIKESFGLYVEICDALGVLLD 240

Query: 241 HFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILITLG 300
           HFKEMEY DCLRAFD+CANAAKNI++LVEFY WCKDVGIA SSEYPEVQMITHN+LITLG
Sbjct: 241 HFKEMEYVDCLRAFDVCANAAKNINDLVEFYGWCKDVGIARSSEYPEVQMITHNLLITLG 300

Query: 301 GFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVLRTQSRK 360
           GFLKEMSRRPKD++E R EEEMKPLPV E  YDMNEIK LP LENC PV QS+LRTQS K
Sbjct: 301 GFLKEMSRRPKDFDEMRTEEEMKPLPVNESGYDMNEIKALPLLENCAPVPQSILRTQSPK 360

Query: 361 EDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFG 420
           EDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEP+ SS WQTP AEFG
Sbjct: 361 EDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPEVSSAWQTPAAEFG 420

Query: 421 KPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFGGSSSSVAVAL 480
           KPDWEL LVETASHLSKQKPDMGGCF PLLLNGMYDQE VRRHVN  QFGGSSSSVAVAL
Sbjct: 421 KPDWELTLVETASHLSKQKPDMGGCFDPLLLNGMYDQETVRRHVNAVQFGGSSSSVAVAL 480

Query: 481 QGQGNAAA-LLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLW 540
           QGQGNAAA +LALPAPDG  EPI YRDPFAASLTVPPPSYVQIAEMEKKQ+LL QEQLLW
Sbjct: 481 QGQGNAAAQVLALPAPDGTAEPIGYRDPFAASLTVPPPSYVQIAEMEKKQELLTQEQLLW 540

Query: 541 KQYGRDGMQGQVGLGKLGGGSGYVGLNPQQIMPHRSAYYYAPF 583
           KQYGRDGMQGQ GLGKLGGGS YV LNPQQIMPH SAYYYA F
Sbjct: 541 KQYGRDGMQGQGGLGKLGGGSAYVDLNPQQIMPHGSAYYYASF 583

BLAST of Clc06G12270 vs. NCBI nr
Match: XP_008443967.1 (PREDICTED: putative clathrin assembly protein At2g25430 [Cucumis melo] >TYK06390.1 putative clathrin assembly protein [Cucumis melo var. makuwa])

HSP 1 Score: 946.4 bits (2445), Expect = 1.1e-271
Identity = 483/563 (85.79%), Postives = 511/563 (90.76%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKAIGAMKDQTTISIAKVAGNM+ ELDVLVVKATSHDE+PADDRYIREIVNLCS
Sbjct: 1   MAQSTIRKAIGAMKDQTTISIAKVAGNMARELDVLVVKATSHDEDPADDRYIREIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
           N SNGYV+ACVVTISKR SKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKG RVL
Sbjct: 61  NYSNGYVNACVVTISKRLSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGTRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYYRSRRQSPWRSMR 180
           DLSGFRDEAHSNSWDHSSFLRFYALYLDELIE LVLEK+VKG D+SYYR +RQS WRS+R
Sbjct: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIETLVLEKKVKGGDESYYRGKRQSSWRSIR 180

Query: 181 PKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVLLD 240
           P+RVVEELNQL+++LDRVLACRP GMAKSSRLVITALNLVIKESF LYIEICDALGVLLD
Sbjct: 181 PERVVEELNQLIRMLDRVLACRPNGMAKSSRLVITALNLVIKESFRLYIEICDALGVLLD 240

Query: 241 HFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILITLG 300
            FKEMEY DCLR FD+CANA KNIDELVEFY WCKDVGIA SSEYP V+MIT+N+LITLG
Sbjct: 241 QFKEMEYADCLRVFDVCANAVKNIDELVEFYCWCKDVGIARSSEYPGVRMITNNLLITLG 300

Query: 301 GFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVLRTQSRK 360
           GFLKEM+RRPKDYNE R  +EMKP+ VKE  YDMNEIK LPP ENC PV QSVL  QS K
Sbjct: 301 GFLKEMTRRPKDYNEMR-TKEMKPVSVKESRYDMNEIKALPPPENCAPVSQSVL--QSLK 360

Query: 361 EDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFG 420
           EDLVNLRED VSADEQG KLA+A FSGPP TNPNGSWETFS NHEP+ SSVWQT  AEFG
Sbjct: 361 EDLVNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSVWQTRAAEFG 420

Query: 421 KPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFGGSSSSVAVAL 480
           KPDWELALVETASHLSKQ+PDMGGCF P+LLNGMYDQE VRRHVNVEQ GGSSSSVAVAL
Sbjct: 421 KPDWELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGGSSSSVAVAL 480

Query: 481 QGQGNAAA-LLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLW 540
           QGQG+A + +LALPAPDG VE +  RDPFAASL VPPPSYVQIAEMEKKQQLL QEQLLW
Sbjct: 481 QGQGSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQLLAQEQLLW 540

Query: 541 KQYGRDGMQGQVGLGKLGGGSGY 563
           KQYGRDGMQGQVGL K+G GS Y
Sbjct: 541 KQYGRDGMQGQVGLEKVGNGSAY 560

BLAST of Clc06G12270 vs. NCBI nr
Match: XP_023529315.1 (putative clathrin assembly protein At2g25430 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 884.0 bits (2283), Expect = 6.9e-253
Identity = 456/593 (76.90%), Postives = 506/593 (85.33%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKA+GA+KDQT+ISIAKVAGN + EL+VLVVKATSHDE+PAD RYIREIVNLCS
Sbjct: 1   MAQSTIRKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
             SNGYVSACV+T+SKR SKTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATRKG+RVL
Sbjct: 61  T-SNGYVSACVITVSKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYY----------RS 180
           DLSGFRDEAHSNSWDHSSF+R YALYLDE+ ENLVLEKR++G D + +          + 
Sbjct: 121 DLSGFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRMRGSDDTNFDRRDEFDNVRQR 180

Query: 181 RRQSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIE 240
           RRQSPWRSMRP+ VV+EL+QLL++LDRVLACRP GMAKSSRLVITAL+LV+ +SFGLY+E
Sbjct: 181 RRQSPWRSMRPESVVDELSQLLRILDRVLACRPTGMAKSSRLVITALDLVVNDSFGLYVE 240

Query: 241 ICDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQM 300
           IC+ LG LLD FKEMEYGDCLRAFD+CANAAK IDEL EFY WCKDVGIA SSEYPE Q 
Sbjct: 241 ICEGLGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQR 300

Query: 301 ITHNILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVM 360
           IT N+L+TLGGFLKEMSRRPKDYNE R EE++K LP +E E  MNEIK LPPLENC P +
Sbjct: 301 ITCNLLVTLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL 360

Query: 361 QSVLRTQSRKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASS 420
           Q      SR  DLVNLREDGVSADEQGNKLALALFSGPP  NPNGSWETFSS+ +P+ SS
Sbjct: 361 Q----PPSRPIDLVNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSS 420

Query: 421 VWQTPVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFG 480
            WQTP AE GKP+WELALVETAS+LSKQK DMGG F PLLLNGMYD   +R+H N EQFG
Sbjct: 421 AWQTPAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRKHANAEQFG 480

Query: 481 GSSSSVAVALQGQGNAAA-LLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQ 540
           GSSSS  VAL+GQG  AA +LALPAPDG V+PI  +DPFAASLTVPPPSYVQIAEME KQ
Sbjct: 481 GSSSS--VALKGQGKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEMKQ 540

Query: 541 QLLMQEQLLWKQYGRDGMQGQVGLGKLGGGSGYVGLNPQQIMPHRSAYYYAPF 583
           QLLMQEQLLW+QYGRDGM GQVGL KL GGS Y+G NPQ I P  SAY+YAPF
Sbjct: 541 QLLMQEQLLWQQYGRDGMHGQVGLVKLAGGSAYIGPNPQPIAPQSSAYHYAPF 585

BLAST of Clc06G12270 vs. NCBI nr
Match: XP_022934011.1 (probable clathrin assembly protein At4g32285 [Cucurbita moschata])

HSP 1 Score: 883.6 bits (2282), Expect = 9.0e-253
Identity = 454/592 (76.69%), Postives = 505/592 (85.30%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKA+GA+KDQT+ISIAKVAGN + EL+VLVVKATSHDE+PAD RYIREIVNLCS
Sbjct: 1   MAQSTIRKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
             SNGYVSACV+TISKR SKTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATRKG+RVL
Sbjct: 61  T-SNGYVSACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYY----------RS 180
           DLS FRDEAHSNSWDHSSF+R YALYLDE+ ENLVLEKR++G D + +          + 
Sbjct: 121 DLSSFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRIRGSDDTNFDRRDEFDNVRQR 180

Query: 181 RRQSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIE 240
           RRQSPWRSMRP+ VV+EL+QLL++LDR+LACRP GMAKSSRLVITAL+LV+ +SFGLY+E
Sbjct: 181 RRQSPWRSMRPESVVDELSQLLRILDRILACRPTGMAKSSRLVITALDLVVNDSFGLYVE 240

Query: 241 ICDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQM 300
           IC+ALG LLD FKEMEYGDCLRAFD+CANAAK IDEL EFY WCKDVGIA SSEYPE Q 
Sbjct: 241 ICEALGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQR 300

Query: 301 ITHNILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVM 360
           IT N+L+TLGGFLKEMSRRPKDYNE R EE++K LP +E E  MNEIK LPPLENC P +
Sbjct: 301 ITCNLLVTLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL 360

Query: 361 QSVLRTQSRKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASS 420
           Q      SR EDLVNLREDGVSADEQGNKLALALFSGPP  NPNGSWETFSS+ +P+ SS
Sbjct: 361 Q----PPSRPEDLVNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSS 420

Query: 421 VWQTPVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFG 480
            WQTP AE GKP+WELALVETAS+LSKQK DMGG F PLLLNGMYD   +R+H N EQF 
Sbjct: 421 AWQTPAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQFD 480

Query: 481 GSSSSVAVALQGQGNAAALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQ 540
           GSSSSVA+  Q +  AA +LALPAPDG V+PI  +DPFAASLTVPPPSYVQIAEMEKKQQ
Sbjct: 481 GSSSSVAMKGQSK-TAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQQ 540

Query: 541 LLMQEQLLWKQYGRDGMQGQVGLGKLGGGSGYVGLNPQQIMPHRSAYYYAPF 583
           LLMQEQLLW+QYGRDGM GQVGL KL GGS Y+G NPQ I P  SAY+YAPF
Sbjct: 541 LLMQEQLLWQQYGRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

BLAST of Clc06G12270 vs. NCBI nr
Match: XP_022145098.1 (probable clathrin assembly protein At4g32285 [Momordica charantia])

HSP 1 Score: 881.3 bits (2276), Expect = 4.5e-252
Identity = 452/591 (76.48%), Postives = 513/591 (86.80%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKAIGA+KDQT+ISIAKVAGN +PEL+VLVVKATSHDE+P DDRYI EIVNLCS
Sbjct: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
           + SNGYVSACVVTISKR +KTRDWIVALKAL+VVHRVLIDGHPSFGEEIVYATR+GMRVL
Sbjct: 61  H-SNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKG------VDKSYY--RSRR 180
           +LSGFRDEAHSNSWDHS F+R YALYLD +IE+LVLEK+V+G       D+S Y  + RR
Sbjct: 121 NLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRR 180

Query: 181 QSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEIC 240
           QSPWRSMRP+RV++E +QLL++LDRVLACRP GMAKSSRLVI A++L +KESFGLYIEIC
Sbjct: 181 QSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEIC 240

Query: 241 DALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMIT 300
           +ALGVLLDHFKEMEYGDCLRAFD+  NAAK ID+L+EFYAWCKD+GIA SSEYPEVQ IT
Sbjct: 241 EALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRIT 300

Query: 301 HNILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQS 360
           HN+L TLG FLKEM RRPKD+NE R EE++K  P  + E DMNE+K LPP +N  P + S
Sbjct: 301 HNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHS 360

Query: 361 VL-RTQSRKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSV 420
           VL +T+  KEDLVNLRED VSADEQGNKLALALFSGPPITNP+GSWETFSSN +P+ SS 
Sbjct: 361 VLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSA 420

Query: 421 WQTPVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFGG 480
           W+TP AE GK DWELALVETAS+LSKQK DMGG F PLLLN MYDQ  +RRHVN EQFGG
Sbjct: 421 WKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGG 480

Query: 481 SSSSVAVALQGQGNAAALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQL 540
           SSSSVA+ +QG+  AA +LALPAPDG V P+ +RDPFAASLTVPPPSYVQIAEMEKKQQL
Sbjct: 481 SSSSVALQVQGKTXAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQL 540

Query: 541 LMQEQLLWKQYGRDGMQGQVGLGKLGGGSGYVGLNPQQIMPHRSAYYYAPF 583
           L+QEQL+W+QYGR+GM GQVGL K+GGGS +V +    IMPH SAYYYAPF
Sbjct: 541 LVQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587

BLAST of Clc06G12270 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 580.5 bits (1495), Expect = 2.1e-164
Identity = 324/635 (51.02%), Postives = 418/635 (65.83%), Query Frame = 0

Query: 5   TIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCSNCSN 64
           ++RKAIG +KDQT+I IAKVA NM+P+L+V +VKATSHD++ + D+YIREI++L S  S 
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTS-LSR 63

Query: 65  GYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVLDLSG 124
           GYV ACV ++S+R  KTRDWIVALKAL++VHR+L +G P F EEI+YATR+G R+L++S 
Sbjct: 64  GYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSD 123

Query: 125 FRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVK-----------GVDKSYYRSRRQ 184
           FRDEAHS+SWDHS+F+R YA YLD+ +E  + E+R +             D  Y RSR  
Sbjct: 124 FRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDD 183

Query: 185 -----------------------------------------SPWRSMRPKRVVEELNQLL 244
                                                    +P R M P+R+  ++  L 
Sbjct: 184 FRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQ 243

Query: 245 QLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVLLDHFKEMEYGDCLR 304
           +LLDR L+CRP G+AK+SR+++ A+  V+KESF LY +IC+ L VLLD F +MEY DC++
Sbjct: 244 RLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVK 303

Query: 305 AFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILITLGGFLKEMSRRPKD 364
           AFD  A+AAK IDEL+ FY WCKD G+A SSEYPEVQ IT  +L TL  F+++ ++R K 
Sbjct: 304 AFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKS 363

Query: 365 YNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVLRTQSRK----EDLVNLRE 424
                +E    P P  E   DMNEIK LPP EN  P        + ++    +DLVNLRE
Sbjct: 364 PERKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLRE 423

Query: 425 DGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFGKPDWELAL 484
           D VS D+QGNK ALALF+GPP    NG WE FSS++  + +S WQ P AE GK DWELAL
Sbjct: 424 DDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAWQNPAAELGKADWELAL 483

Query: 485 VETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQF-GGSSSSVAVALQGQGNAA 544
           VETAS+L  QK  MGG   PLLLNGMYDQ  VR+HV+  +  GGSSSSVA+ L G+ N +
Sbjct: 484 VETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGKVN-S 543

Query: 545 ALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWKQYGRDGM 580
            +LALPAPDG V+ +  +DPFAASLT+PPPSYVQ+AEM+KKQ LL QEQ LW+QY ++GM
Sbjct: 544 HILALPAPDGTVQKVN-QDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGM 603

BLAST of Clc06G12270 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 573.5 bits (1477), Expect = 2.6e-162
Identity = 333/655 (50.84%), Postives = 419/655 (63.97%), Query Frame = 0

Query: 5   TIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCSNCSN 64
           +IRKAIGA+KDQT+I IAKVA NM+P+L+V +VKATSHD++PA ++YIREI+NL S  S 
Sbjct: 4   SIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS-LSR 63

Query: 65  GYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVLDLSG 124
           GY+ ACV ++S+R SKTRDW+VALKAL++VHR+L +G P F EEI+Y+TR+G R+L++S 
Sbjct: 64  GYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLNMSD 123

Query: 125 FRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGV--------------DKSYYRS 184
           FRDEAHS+SWDHS+F+R YA YLD+ +E L L +R  GV              D  Y R 
Sbjct: 124 FRDEAHSSSWDHSAFVRTYAGYLDQRLE-LALFERKSGVSVNSGGNSSHHSNNDDRYGRG 183

Query: 185 RRQ--------------------------------------------------------- 244
           R                                                           
Sbjct: 184 RDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGRDEKK 243

Query: 245 --SPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEI 304
             +P R M P+R+  ++  L +LLDR L+ RP G+AK+SR+++ AL  V++ESF LY +I
Sbjct: 244 VVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKLYADI 303

Query: 305 CDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMI 364
           C+ L VLLD F +MEY DC++AFD  A+AAK IDEL+ FY WCK+ G+A SSEYPEVQ I
Sbjct: 304 CEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPEVQRI 363

Query: 365 THNILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQ 424
           T  +L TL  F+++ ++R K      +E     +  +E E DMNEIK LPP EN  P   
Sbjct: 364 TSKLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYTPPPP 423

Query: 425 SVLRTQSRK----EDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPD 484
                Q  K    EDLVNLRED V+AD+QGNK ALALF+GPP    NG WE FSSN    
Sbjct: 424 PEPEPQPEKPQFTEDLVNLREDEVTADDQGNKFALALFAGPP--GNNGKWEAFSSN---G 483

Query: 485 ASSVWQTPVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVE 544
            +S WQ P AE GK DWELALVET S+L KQ   +GG F  LLLNGMYDQ MVR+HV+  
Sbjct: 484 VTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQHVSTS 543

Query: 545 QF-GGSSSSVAVALQGQGNAAALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEME 580
           Q  GGS+SSVA+ L G+ N   +LALPAPDG VE +  +DPFAASLT+PPPSYVQ+AEME
Sbjct: 544 QLTGGSASSVALPLPGKTN-NQVLALPAPDGTVEKVN-QDPFAASLTIPPPSYVQMAEME 603

BLAST of Clc06G12270 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 394.8 bits (1013), Expect = 1.7e-108
Identity = 243/607 (40.03%), Postives = 354/607 (58.32%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMS--PELDVLVVKATSHDENPADDRYIREIVNL 60
           M  + +++AIGA+KDQT++ +AKV G  S   EL++ VVKAT HD+ PA+D+YIREI+ L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  CSNCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMR 120
            S  S  YVSACV T+S+R +KT++W VALK LI++ R+L DG  ++ +EI +ATR+G R
Sbjct: 61  TS-YSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTR 120

Query: 121 VLDLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVK-------GVD------- 180
           +L++S FRD + S+SWD+S+F+R YALYLDE ++  +  +R K       G D       
Sbjct: 121 LLNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEED 180

Query: 181 -----KSYYRSR----RQSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVIT 240
                 +  RS+    +  P   M+ +++   +  L QLLDR LACRP G AK++R+VI 
Sbjct: 181 DHRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIV 240

Query: 241 ALNLVIKESFGLYIEICDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCK 300
           A+  ++KESF LY  I + +GVL++ F E++  D ++ ++I    +K  DEL  FY WCK
Sbjct: 241 AMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCK 300

Query: 301 DVGIAGSSEYPEVQMITHNILITLGGFLKEMS-------RRPKDYNETRMEEEMKPLPVK 360
           ++ +A SSEYPE++ IT   L  +  F+++ S       +     +    EEE K   ++
Sbjct: 301 NMAVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQ 360

Query: 361 ELEYDMNEIKTLPPLE-----------NCVPVMQSVLRTQSRKEDLVNLRED-GVSADEQ 420
           E + D+N IK LP  E                ++ V+  Q ++ DL++L ++ GV+A   
Sbjct: 361 ENQEDLNSIKALPAPEQKEEEEEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTV 420

Query: 421 GNKLALALFSGPPITN--PNGSWETFSSNHEPDASSVWQTPVAEFGKPDWELALVETASH 480
           G+ LALALF G   T       WE F+ N                   DWE  LV +A+ 
Sbjct: 421 GDSLALALFDGVVGTESASGPGWEAFNDN-----------------SADWETDLVRSATR 480

Query: 481 LSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVE-QFGGSSSSVAVALQGQGN-AAALLAL 540
           LS QK ++GG F  LLL+GMY    V   V     +G S S+ +VA    G+ AA++LAL
Sbjct: 481 LSGQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLAL 540

Query: 541 PAPDGMVEPIRYR----DPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWKQYGRDGMQ 556
           PAP       R      DPFAASL V PP+YVQ+ +MEKKQ+LLM+EQ++W QY R G Q
Sbjct: 541 PAPPPTANGNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQ 589

BLAST of Clc06G12270 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.1e-107
Identity = 238/599 (39.73%), Postives = 355/599 (59.27%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMS--PELDVLVVKATSHDENPADDRYIREIVNL 60
           M  +  ++AIGA+KDQT++ +AKV G  +   ELDV +VKAT H+E PA+++YIREI++L
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  CSNCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMR 120
            S  S  Y++ACV T+S+R +KT+ W VALK LI++ R+L +G  ++ +EI +ATR+G R
Sbjct: 61  TS-YSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTR 120

Query: 121 VLDLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVK----------------- 180
           +L++S FRD + SNSWD+S+F+R YALYLDE ++  +  +  K                 
Sbjct: 121 LLNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQ 180

Query: 181 -GVDKSYYRSRRQSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLV 240
              D S     R  P   M+ +++   +  L QLLDR LACRP G A+++R+VI AL  +
Sbjct: 181 AAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPI 240

Query: 241 IKESFGLYIEICDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIA 300
           +KESF +Y ++ + +G+L++ F E++  D ++ +DI    +K  +EL +FY+WCK++GIA
Sbjct: 241 VKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIA 300

Query: 301 GSSEYPEVQMITHNILITLGGFLKEMS--RRPKDYNETRME-----EEMKPLPVKELEYD 360
            SSEYPE++ IT   L  +  F+++ S     K     + E     +E +   V E + D
Sbjct: 301 RSSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQED 360

Query: 361 MNEIKTL---PPLE--NCVPVMQS-----VLRTQSRKEDLVNL-REDGVSADEQGNKLAL 420
           MN IK L   PP E  +  P  ++     + + Q    DL++L   +G  A + G+ LAL
Sbjct: 361 MNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDSLAL 420

Query: 421 ALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFGKPDWELALVETASHLSKQKPDM 480
           ALF GP           ++S    ++   W+    +    DWE ALV+TA++LS QK ++
Sbjct: 421 ALFDGP-----------YASGSGSESGPGWE--AFKDDSADWETALVQTATNLSGQKSEL 480

Query: 481 GGCFHPLLLNGMYDQEMVRRHVNVE-QFGGSSSSVAVALQGQGN-AAALLALPAPD---- 540
           GG F  LLLNGMY    V   V     +G S S+ ++A    G  AA +LALPAP     
Sbjct: 481 GGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPSTANG 540

Query: 541 --GMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWKQYGRDGMQGQVGL 554
             G +      DPFAASL V PP+YVQ+ +MEKKQ++LM+EQ++W QY RDG QG + L
Sbjct: 541 NAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGHMNL 585

BLAST of Clc06G12270 vs. ExPASy Swiss-Prot
Match: Q9LVD8 (Putative clathrin assembly protein At5g57200 OS=Arabidopsis thaliana OX=3702 GN=At5g57200 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 8.1e-63
Identity = 178/564 (31.56%), Postives = 291/564 (51.60%), Query Frame = 0

Query: 4   TTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCSNCS 63
           T+ RKA GA+KD TT+ +AKV      +LD+ +VKAT+H E+P  +R++R+I +  S   
Sbjct: 5   TSFRKAYGALKDTTTVGLAKVNSEFK-DLDIAIVKATNHVESPPKERHVRKIFSATSVIQ 64

Query: 64  -NGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIV-YATRKGMRVLD 123
               V+ C+  +SKR SKTR+W+VA+K LIV+HR L +G P+F EE++ Y+ R+   +L 
Sbjct: 65  PRADVAYCIHALSKRLSKTRNWVVAMKVLIVIHRTLREGDPTFREELLNYSHRR--HILR 124

Query: 124 LSGFRDEAHSNSWDHSSFLRFYALYLDELIE---NLVLEKRVKGVDKSYYRSRRQSPWRS 183
           +S F+D+    +WD S+++R YAL+L+E +E    L  +   + + K+   + +    R 
Sbjct: 125 ISNFKDDTSPLAWDCSAWVRTYALFLEERLECYRVLKYDIEAERLPKASGAASKTHRTRM 184

Query: 184 MRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVL 243
           +  + ++E+L  L QLL R++ C+P G A S+ L+  AL LV+KESF +Y  I D +  L
Sbjct: 185 LSGEDLLEQLPALQQLLYRLIGCQPEGAAYSNYLIQYALALVLKESFKIYCAINDGIINL 244

Query: 244 LDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILIT 303
           +D F EM   D ++A +I   A +  + L EFY +CK + +A + ++P ++    + L T
Sbjct: 245 VDMFFEMSRHDAVKALNIYKRAGQQAENLAEFYDYCKGLELARNFQFPTLRQPPPSFLAT 304

Query: 304 LGGFLKE----------MSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVP 363
           +  ++KE          +  + K+  E   EEE    P +E   + N     P +E    
Sbjct: 305 MEEYIKEAPQSGSVQKKLEYQEKEEEEQEQEEEQPEEPAEEENQNENTENDQPLIEEEEE 364

Query: 364 VMQSVLRTQSRK-------EDLVNLREDGVSAD--EQGNKLALALFSGPPITNPNGSWET 423
             +  +  +  K       +DL+ L E    A   EQ N  +LA++  PP          
Sbjct: 365 EPKEEIEVEEAKPSPLIDTDDLLGLHEINPKAAEIEQNNAFSLAIY--PP---------- 424

Query: 424 FSSNHEPDASSVWQTPVAEFGKPDWELALVETASHLSKQK-------PDMGGCFHPLLLN 483
               HE  A S     + E G   WELALV   ++ +            +GG F  LLL+
Sbjct: 425 ---GHETSAPSN-SLSLIEAGGSGWELALVTPQNNNNNNNNPRPVIATKLGGGFDNLLLD 484

Query: 484 GMYDQEMVRRHVNVEQFGGSSSSVAVALQGQGNAAALLALPAPDGMVEPIRYRDPFAASL 537
            +Y+ +  RR + +   G    + A+        A   + P P G+      +DPFA S 
Sbjct: 485 SLYEDDTARRQIQLTNAGYGFGATAIP------GALASSNPNPFGV-----QQDPFAMSN 537

BLAST of Clc06G12270 vs. ExPASy TrEMBL
Match: A0A5D3C4X9 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00870 PE=4 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.5e-272
Identity = 483/563 (85.79%), Postives = 511/563 (90.76%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKAIGAMKDQTTISIAKVAGNM+ ELDVLVVKATSHDE+PADDRYIREIVNLCS
Sbjct: 1   MAQSTIRKAIGAMKDQTTISIAKVAGNMARELDVLVVKATSHDEDPADDRYIREIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
           N SNGYV+ACVVTISKR SKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKG RVL
Sbjct: 61  NYSNGYVNACVVTISKRLSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGTRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYYRSRRQSPWRSMR 180
           DLSGFRDEAHSNSWDHSSFLRFYALYLDELIE LVLEK+VKG D+SYYR +RQS WRS+R
Sbjct: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIETLVLEKKVKGGDESYYRGKRQSSWRSIR 180

Query: 181 PKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVLLD 240
           P+RVVEELNQL+++LDRVLACRP GMAKSSRLVITALNLVIKESF LYIEICDALGVLLD
Sbjct: 181 PERVVEELNQLIRMLDRVLACRPNGMAKSSRLVITALNLVIKESFRLYIEICDALGVLLD 240

Query: 241 HFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILITLG 300
            FKEMEY DCLR FD+CANA KNIDELVEFY WCKDVGIA SSEYP V+MIT+N+LITLG
Sbjct: 241 QFKEMEYADCLRVFDVCANAVKNIDELVEFYCWCKDVGIARSSEYPGVRMITNNLLITLG 300

Query: 301 GFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVLRTQSRK 360
           GFLKEM+RRPKDYNE R  +EMKP+ VKE  YDMNEIK LPP ENC PV QSVL  QS K
Sbjct: 301 GFLKEMTRRPKDYNEMR-TKEMKPVSVKESRYDMNEIKALPPPENCAPVSQSVL--QSLK 360

Query: 361 EDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFG 420
           EDLVNLRED VSADEQG KLA+A FSGPP TNPNGSWETFS NHEP+ SSVWQT  AEFG
Sbjct: 361 EDLVNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSVWQTRAAEFG 420

Query: 421 KPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFGGSSSSVAVAL 480
           KPDWELALVETASHLSKQ+PDMGGCF P+LLNGMYDQE VRRHVNVEQ GGSSSSVAVAL
Sbjct: 421 KPDWELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGGSSSSVAVAL 480

Query: 481 QGQGNAAA-LLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLW 540
           QGQG+A + +LALPAPDG VE +  RDPFAASL VPPPSYVQIAEMEKKQQLL QEQLLW
Sbjct: 481 QGQGSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQLLAQEQLLW 540

Query: 541 KQYGRDGMQGQVGLGKLGGGSGY 563
           KQYGRDGMQGQVGL K+G GS Y
Sbjct: 541 KQYGRDGMQGQVGLEKVGNGSAY 560

BLAST of Clc06G12270 vs. ExPASy TrEMBL
Match: A0A1S3B992 (putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC103487428 PE=4 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.5e-272
Identity = 483/563 (85.79%), Postives = 511/563 (90.76%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKAIGAMKDQTTISIAKVAGNM+ ELDVLVVKATSHDE+PADDRYIREIVNLCS
Sbjct: 1   MAQSTIRKAIGAMKDQTTISIAKVAGNMARELDVLVVKATSHDEDPADDRYIREIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
           N SNGYV+ACVVTISKR SKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKG RVL
Sbjct: 61  NYSNGYVNACVVTISKRLSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGTRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYYRSRRQSPWRSMR 180
           DLSGFRDEAHSNSWDHSSFLRFYALYLDELIE LVLEK+VKG D+SYYR +RQS WRS+R
Sbjct: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIETLVLEKKVKGGDESYYRGKRQSSWRSIR 180

Query: 181 PKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVLLD 240
           P+RVVEELNQL+++LDRVLACRP GMAKSSRLVITALNLVIKESF LYIEICDALGVLLD
Sbjct: 181 PERVVEELNQLIRMLDRVLACRPNGMAKSSRLVITALNLVIKESFRLYIEICDALGVLLD 240

Query: 241 HFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILITLG 300
            FKEMEY DCLR FD+CANA KNIDELVEFY WCKDVGIA SSEYP V+MIT+N+LITLG
Sbjct: 241 QFKEMEYADCLRVFDVCANAVKNIDELVEFYCWCKDVGIARSSEYPGVRMITNNLLITLG 300

Query: 301 GFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVLRTQSRK 360
           GFLKEM+RRPKDYNE R  +EMKP+ VKE  YDMNEIK LPP ENC PV QSVL  QS K
Sbjct: 301 GFLKEMTRRPKDYNEMR-TKEMKPVSVKESRYDMNEIKALPPPENCAPVSQSVL--QSLK 360

Query: 361 EDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFG 420
           EDLVNLRED VSADEQG KLA+A FSGPP TNPNGSWETFS NHEP+ SSVWQT  AEFG
Sbjct: 361 EDLVNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSVWQTRAAEFG 420

Query: 421 KPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFGGSSSSVAVAL 480
           KPDWELALVETASHLSKQ+PDMGGCF P+LLNGMYDQE VRRHVNVEQ GGSSSSVAVAL
Sbjct: 421 KPDWELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGGSSSSVAVAL 480

Query: 481 QGQGNAAA-LLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLW 540
           QGQG+A + +LALPAPDG VE +  RDPFAASL VPPPSYVQIAEMEKKQQLL QEQLLW
Sbjct: 481 QGQGSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQLLAQEQLLW 540

Query: 541 KQYGRDGMQGQVGLGKLGGGSGY 563
           KQYGRDGMQGQVGL K+G GS Y
Sbjct: 541 KQYGRDGMQGQVGLEKVGNGSAY 560

BLAST of Clc06G12270 vs. ExPASy TrEMBL
Match: A0A6J1F0M3 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111441244 PE=4 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 4.4e-253
Identity = 454/592 (76.69%), Postives = 505/592 (85.30%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKA+GA+KDQT+ISIAKVAGN + EL+VLVVKATSHDE+PAD RYIREIVNLCS
Sbjct: 1   MAQSTIRKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
             SNGYVSACV+TISKR SKTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATRKG+RVL
Sbjct: 61  T-SNGYVSACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYY----------RS 180
           DLS FRDEAHSNSWDHSSF+R YALYLDE+ ENLVLEKR++G D + +          + 
Sbjct: 121 DLSSFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRIRGSDDTNFDRRDEFDNVRQR 180

Query: 181 RRQSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIE 240
           RRQSPWRSMRP+ VV+EL+QLL++LDR+LACRP GMAKSSRLVITAL+LV+ +SFGLY+E
Sbjct: 181 RRQSPWRSMRPESVVDELSQLLRILDRILACRPTGMAKSSRLVITALDLVVNDSFGLYVE 240

Query: 241 ICDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQM 300
           IC+ALG LLD FKEMEYGDCLRAFD+CANAAK IDEL EFY WCKDVGIA SSEYPE Q 
Sbjct: 241 ICEALGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQR 300

Query: 301 ITHNILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVM 360
           IT N+L+TLGGFLKEMSRRPKDYNE R EE++K LP +E E  MNEIK LPPLENC P +
Sbjct: 301 ITCNLLVTLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL 360

Query: 361 QSVLRTQSRKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASS 420
           Q      SR EDLVNLREDGVSADEQGNKLALALFSGPP  NPNGSWETFSS+ +P+ SS
Sbjct: 361 Q----PPSRPEDLVNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSS 420

Query: 421 VWQTPVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFG 480
            WQTP AE GKP+WELALVETAS+LSKQK DMGG F PLLLNGMYD   +R+H N EQF 
Sbjct: 421 AWQTPAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQFD 480

Query: 481 GSSSSVAVALQGQGNAAALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQ 540
           GSSSSVA+  Q +  AA +LALPAPDG V+PI  +DPFAASLTVPPPSYVQIAEMEKKQQ
Sbjct: 481 GSSSSVAMKGQSK-TAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQQ 540

Query: 541 LLMQEQLLWKQYGRDGMQGQVGLGKLGGGSGYVGLNPQQIMPHRSAYYYAPF 583
           LLMQEQLLW+QYGRDGM GQVGL KL GGS Y+G NPQ I P  SAY+YAPF
Sbjct: 541 LLMQEQLLWQQYGRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

BLAST of Clc06G12270 vs. ExPASy TrEMBL
Match: A0A6J1CV16 (probable clathrin assembly protein At4g32285 OS=Momordica charantia OX=3673 GN=LOC111014605 PE=4 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 2.2e-252
Identity = 452/591 (76.48%), Postives = 513/591 (86.80%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKAIGA+KDQT+ISIAKVAGN +PEL+VLVVKATSHDE+P DDRYI EIVNLCS
Sbjct: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
           + SNGYVSACVVTISKR +KTRDWIVALKAL+VVHRVLIDGHPSFGEEIVYATR+GMRVL
Sbjct: 61  H-SNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKG------VDKSYY--RSRR 180
           +LSGFRDEAHSNSWDHS F+R YALYLD +IE+LVLEK+V+G       D+S Y  + RR
Sbjct: 121 NLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRR 180

Query: 181 QSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEIC 240
           QSPWRSMRP+RV++E +QLL++LDRVLACRP GMAKSSRLVI A++L +KESFGLYIEIC
Sbjct: 181 QSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEIC 240

Query: 241 DALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMIT 300
           +ALGVLLDHFKEMEYGDCLRAFD+  NAAK ID+L+EFYAWCKD+GIA SSEYPEVQ IT
Sbjct: 241 EALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRIT 300

Query: 301 HNILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQS 360
           HN+L TLG FLKEM RRPKD+NE R EE++K  P  + E DMNE+K LPP +N  P + S
Sbjct: 301 HNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHS 360

Query: 361 VL-RTQSRKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSV 420
           VL +T+  KEDLVNLRED VSADEQGNKLALALFSGPPITNP+GSWETFSSN +P+ SS 
Sbjct: 361 VLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSA 420

Query: 421 WQTPVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFGG 480
           W+TP AE GK DWELALVETAS+LSKQK DMGG F PLLLN MYDQ  +RRHVN EQFGG
Sbjct: 421 WKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGG 480

Query: 481 SSSSVAVALQGQGNAAALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQL 540
           SSSSVA+ +QG+  AA +LALPAPDG V P+ +RDPFAASLTVPPPSYVQIAEMEKKQQL
Sbjct: 481 SSSSVALQVQGKTXAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQL 540

Query: 541 LMQEQLLWKQYGRDGMQGQVGLGKLGGGSGYVGLNPQQIMPHRSAYYYAPF 583
           L+QEQL+W+QYGR+GM GQVGL K+GGGS +V +    IMPH SAYYYAPF
Sbjct: 541 LVQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587

BLAST of Clc06G12270 vs. ExPASy TrEMBL
Match: A0A6J1I3H3 (probable clathrin assembly protein At4g32285 OS=Cucurbita maxima OX=3661 GN=LOC111470668 PE=4 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.2e-250
Identity = 451/589 (76.57%), Postives = 503/589 (85.40%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCS 60
           MAQ+TIRKA+GA+KDQT+IS+AKVAGN + EL+VLVVKATSHDE+PAD RYIREIVN CS
Sbjct: 1   MAQSTIRKALGAVKDQTSISMAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNWCS 60

Query: 61  NCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVL 120
             SNGYVSACV+TISKR SKTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATRKG+RVL
Sbjct: 61  T-SNGYVSACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVL 120

Query: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGVDKSYYRSR------RQS 180
           DLS FRDEAHSNSWDHSSF+R YALYLDE+ ENLVLEKR++G D +Y+  R      RQS
Sbjct: 121 DLSSFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRMRGSDDTYFDRRDEFDNVRQS 180

Query: 181 PWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDA 240
           PWRSMRP+ VV+EL+QLL++LDR+LACRP GMAKSSRLVI+AL+LV+ +SFGLY+EIC A
Sbjct: 181 PWRSMRPESVVDELSQLLRILDRLLACRPTGMAKSSRLVISALDLVVNDSFGLYVEICKA 240

Query: 241 LGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHN 300
           LG LLD FKEMEYGDCLRAFD+CANAAK IDEL EFY WCKDVGIA SSE+PE Q IT N
Sbjct: 241 LGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEFPEAQRITSN 300

Query: 301 ILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVL 360
           +L+TLGGFLKEMSRRPKDYNE R EE++K LP +E E  MNEIK LPPLENC P +Q   
Sbjct: 301 LLVTLGGFLKEMSRRPKDYNERRREEKVKSLPTEEPECYMNEIKALPPLENCSPTLQ--- 360

Query: 361 RTQSRKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQT 420
              SR EDLVNLREDGVSADEQGNKLA ALFSGPP  NPNGSWETFSS+ +P+ SS WQT
Sbjct: 361 -PPSRPEDLVNLREDGVSADEQGNKLAWALFSGPP-ANPNGSWETFSSDKQPEVSSAWQT 420

Query: 421 PVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQFGGSSS 480
           P AE GKP+WELALVETAS+LSKQK DMGG F PLLLNGMYD   +R+H N EQFGGSSS
Sbjct: 421 PAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANGEQFGGSSS 480

Query: 481 SVAVALQGQGNAAA-LLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLM 540
           S  VAL+GQG  AA +LALPAPDG V+P+  +DPFA SLTVPPPSYVQIAEMEKKQQLLM
Sbjct: 481 S--VALKGQGKTAAQVLALPAPDGTVKPVGVQDPFAVSLTVPPPSYVQIAEMEKKQQLLM 540

Query: 541 QEQLLWKQYGRDGMQGQVGLGKLGGGSGYVGLNPQQIMPHRSAYYYAPF 583
           QEQLLW+QYG DGM GQVGL KL GGS Y+G NPQ I P  SAY+YAPF
Sbjct: 541 QEQLLWQQYGIDGMHGQVGLVKLAGGSVYIGPNPQPIAPQSSAYHYAPF 581

BLAST of Clc06G12270 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.5e-165
Identity = 324/635 (51.02%), Postives = 418/635 (65.83%), Query Frame = 0

Query: 5   TIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCSNCSN 64
           ++RKAIG +KDQT+I IAKVA NM+P+L+V +VKATSHD++ + D+YIREI++L S  S 
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTS-LSR 63

Query: 65  GYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVLDLSG 124
           GYV ACV ++S+R  KTRDWIVALKAL++VHR+L +G P F EEI+YATR+G R+L++S 
Sbjct: 64  GYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSD 123

Query: 125 FRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVK-----------GVDKSYYRSRRQ 184
           FRDEAHS+SWDHS+F+R YA YLD+ +E  + E+R +             D  Y RSR  
Sbjct: 124 FRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDD 183

Query: 185 -----------------------------------------SPWRSMRPKRVVEELNQLL 244
                                                    +P R M P+R+  ++  L 
Sbjct: 184 FRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQ 243

Query: 245 QLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVLLDHFKEMEYGDCLR 304
           +LLDR L+CRP G+AK+SR+++ A+  V+KESF LY +IC+ L VLLD F +MEY DC++
Sbjct: 244 RLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVK 303

Query: 305 AFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILITLGGFLKEMSRRPKD 364
           AFD  A+AAK IDEL+ FY WCKD G+A SSEYPEVQ IT  +L TL  F+++ ++R K 
Sbjct: 304 AFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKS 363

Query: 365 YNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVLRTQSRK----EDLVNLRE 424
                +E    P P  E   DMNEIK LPP EN  P        + ++    +DLVNLRE
Sbjct: 364 PERKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLRE 423

Query: 425 DGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFGKPDWELAL 484
           D VS D+QGNK ALALF+GPP    NG WE FSS++  + +S WQ P AE GK DWELAL
Sbjct: 424 DDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAWQNPAAELGKADWELAL 483

Query: 485 VETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQF-GGSSSSVAVALQGQGNAA 544
           VETAS+L  QK  MGG   PLLLNGMYDQ  VR+HV+  +  GGSSSSVA+ L G+ N +
Sbjct: 484 VETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGKVN-S 543

Query: 545 ALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWKQYGRDGM 580
            +LALPAPDG V+ +  +DPFAASLT+PPPSYVQ+AEM+KKQ LL QEQ LW+QY ++GM
Sbjct: 544 HILALPAPDGTVQKVN-QDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGM 603

BLAST of Clc06G12270 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.5e-165
Identity = 324/635 (51.02%), Postives = 418/635 (65.83%), Query Frame = 0

Query: 5   TIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCSNCSN 64
           ++RKAIG +KDQT+I IAKVA NM+P+L+V +VKATSHD++ + D+YIREI++L S  S 
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTS-LSR 63

Query: 65  GYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVLDLSG 124
           GYV ACV ++S+R  KTRDWIVALKAL++VHR+L +G P F EEI+YATR+G R+L++S 
Sbjct: 64  GYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSD 123

Query: 125 FRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVK-----------GVDKSYYRSRRQ 184
           FRDEAHS+SWDHS+F+R YA YLD+ +E  + E+R +             D  Y RSR  
Sbjct: 124 FRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDD 183

Query: 185 -----------------------------------------SPWRSMRPKRVVEELNQLL 244
                                                    +P R M P+R+  ++  L 
Sbjct: 184 FRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQ 243

Query: 245 QLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEICDALGVLLDHFKEMEYGDCLR 304
           +LLDR L+CRP G+AK+SR+++ A+  V+KESF LY +IC+ L VLLD F +MEY DC++
Sbjct: 244 RLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVK 303

Query: 305 AFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMITHNILITLGGFLKEMSRRPKD 364
           AFD  A+AAK IDEL+ FY WCKD G+A SSEYPEVQ IT  +L TL  F+++ ++R K 
Sbjct: 304 AFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKS 363

Query: 365 YNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQSVLRTQSRK----EDLVNLRE 424
                +E    P P  E   DMNEIK LPP EN  P        + ++    +DLVNLRE
Sbjct: 364 PERKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLRE 423

Query: 425 DGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFGKPDWELAL 484
           D VS D+QGNK ALALF+GPP    NG WE FSS++  + +S WQ P AE GK DWELAL
Sbjct: 424 DDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAWQNPAAELGKADWELAL 483

Query: 485 VETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVEQF-GGSSSSVAVALQGQGNAA 544
           VETAS+L  QK  MGG   PLLLNGMYDQ  VR+HV+  +  GGSSSSVA+ L G+ N +
Sbjct: 484 VETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGKVN-S 543

Query: 545 ALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWKQYGRDGM 580
            +LALPAPDG V+ +  +DPFAASLT+PPPSYVQ+AEM+KKQ LL QEQ LW+QY ++GM
Sbjct: 544 HILALPAPDGTVQKVN-QDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGM 603

BLAST of Clc06G12270 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 573.5 bits (1477), Expect = 1.9e-163
Identity = 333/655 (50.84%), Postives = 419/655 (63.97%), Query Frame = 0

Query: 5   TIRKAIGAMKDQTTISIAKVAGNMSPELDVLVVKATSHDENPADDRYIREIVNLCSNCSN 64
           +IRKAIGA+KDQT+I IAKVA NM+P+L+V +VKATSHD++PA ++YIREI+NL S  S 
Sbjct: 4   SIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS-LSR 63

Query: 65  GYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMRVLDLSG 124
           GY+ ACV ++S+R SKTRDW+VALKAL++VHR+L +G P F EEI+Y+TR+G R+L++S 
Sbjct: 64  GYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLNMSD 123

Query: 125 FRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVKGV--------------DKSYYRS 184
           FRDEAHS+SWDHS+F+R YA YLD+ +E L L +R  GV              D  Y R 
Sbjct: 124 FRDEAHSSSWDHSAFVRTYAGYLDQRLE-LALFERKSGVSVNSGGNSSHHSNNDDRYGRG 183

Query: 185 RRQ--------------------------------------------------------- 244
           R                                                           
Sbjct: 184 RDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGRDEKK 243

Query: 245 --SPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLVIKESFGLYIEI 304
             +P R M P+R+  ++  L +LLDR L+ RP G+AK+SR+++ AL  V++ESF LY +I
Sbjct: 244 VVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKLYADI 303

Query: 305 CDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIAGSSEYPEVQMI 364
           C+ L VLLD F +MEY DC++AFD  A+AAK IDEL+ FY WCK+ G+A SSEYPEVQ I
Sbjct: 304 CEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPEVQRI 363

Query: 365 THNILITLGGFLKEMSRRPKDYNETRMEEEMKPLPVKELEYDMNEIKTLPPLENCVPVMQ 424
           T  +L TL  F+++ ++R K      +E     +  +E E DMNEIK LPP EN  P   
Sbjct: 364 TSKLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYTPPPP 423

Query: 425 SVLRTQSRK----EDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPD 484
                Q  K    EDLVNLRED V+AD+QGNK ALALF+GPP    NG WE FSSN    
Sbjct: 424 PEPEPQPEKPQFTEDLVNLREDEVTADDQGNKFALALFAGPP--GNNGKWEAFSSN---G 483

Query: 485 ASSVWQTPVAEFGKPDWELALVETASHLSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVE 544
            +S WQ P AE GK DWELALVET S+L KQ   +GG F  LLLNGMYDQ MVR+HV+  
Sbjct: 484 VTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQHVSTS 543

Query: 545 QF-GGSSSSVAVALQGQGNAAALLALPAPDGMVEPIRYRDPFAASLTVPPPSYVQIAEME 580
           Q  GGS+SSVA+ L G+ N   +LALPAPDG VE +  +DPFAASLT+PPPSYVQ+AEME
Sbjct: 544 QLTGGSASSVALPLPGKTN-NQVLALPAPDGTVEKVN-QDPFAASLTIPPPSYVQMAEME 603

BLAST of Clc06G12270 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 394.8 bits (1013), Expect = 1.2e-109
Identity = 243/607 (40.03%), Postives = 354/607 (58.32%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMS--PELDVLVVKATSHDENPADDRYIREIVNL 60
           M  + +++AIGA+KDQT++ +AKV G  S   EL++ VVKAT HD+ PA+D+YIREI+ L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  CSNCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMR 120
            S  S  YVSACV T+S+R +KT++W VALK LI++ R+L DG  ++ +EI +ATR+G R
Sbjct: 61  TS-YSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTR 120

Query: 121 VLDLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVK-------GVD------- 180
           +L++S FRD + S+SWD+S+F+R YALYLDE ++  +  +R K       G D       
Sbjct: 121 LLNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEED 180

Query: 181 -----KSYYRSR----RQSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVIT 240
                 +  RS+    +  P   M+ +++   +  L QLLDR LACRP G AK++R+VI 
Sbjct: 181 DHRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIV 240

Query: 241 ALNLVIKESFGLYIEICDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCK 300
           A+  ++KESF LY  I + +GVL++ F E++  D ++ ++I    +K  DEL  FY WCK
Sbjct: 241 AMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCK 300

Query: 301 DVGIAGSSEYPEVQMITHNILITLGGFLKEMS-------RRPKDYNETRMEEEMKPLPVK 360
           ++ +A SSEYPE++ IT   L  +  F+++ S       +     +    EEE K   ++
Sbjct: 301 NMAVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQ 360

Query: 361 ELEYDMNEIKTLPPLE-----------NCVPVMQSVLRTQSRKEDLVNLRED-GVSADEQ 420
           E + D+N IK LP  E                ++ V+  Q ++ DL++L ++ GV+A   
Sbjct: 361 ENQEDLNSIKALPAPEQKEEEEEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTV 420

Query: 421 GNKLALALFSGPPITN--PNGSWETFSSNHEPDASSVWQTPVAEFGKPDWELALVETASH 480
           G+ LALALF G   T       WE F+ N                   DWE  LV +A+ 
Sbjct: 421 GDSLALALFDGVVGTESASGPGWEAFNDN-----------------SADWETDLVRSATR 480

Query: 481 LSKQKPDMGGCFHPLLLNGMYDQEMVRRHVNVE-QFGGSSSSVAVALQGQGN-AAALLAL 540
           LS QK ++GG F  LLL+GMY    V   V     +G S S+ +VA    G+ AA++LAL
Sbjct: 481 LSGQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLAL 540

Query: 541 PAPDGMVEPIRYR----DPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWKQYGRDGMQ 556
           PAP       R      DPFAASL V PP+YVQ+ +MEKKQ+LLM+EQ++W QY R G Q
Sbjct: 541 PAPPPTANGNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQ 589

BLAST of Clc06G12270 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 392.1 bits (1006), Expect = 7.7e-109
Identity = 238/599 (39.73%), Postives = 355/599 (59.27%), Query Frame = 0

Query: 1   MAQTTIRKAIGAMKDQTTISIAKVAGNMS--PELDVLVVKATSHDENPADDRYIREIVNL 60
           M  +  ++AIGA+KDQT++ +AKV G  +   ELDV +VKAT H+E PA+++YIREI++L
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  CSNCSNGYVSACVVTISKRWSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGMR 120
            S  S  Y++ACV T+S+R +KT+ W VALK LI++ R+L +G  ++ +EI +ATR+G R
Sbjct: 61  TS-YSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTR 120

Query: 121 VLDLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRVK----------------- 180
           +L++S FRD + SNSWD+S+F+R YALYLDE ++  +  +  K                 
Sbjct: 121 LLNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQ 180

Query: 181 -GVDKSYYRSRRQSPWRSMRPKRVVEELNQLLQLLDRVLACRPAGMAKSSRLVITALNLV 240
              D S     R  P   M+ +++   +  L QLLDR LACRP G A+++R+VI AL  +
Sbjct: 181 AAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPI 240

Query: 241 IKESFGLYIEICDALGVLLDHFKEMEYGDCLRAFDICANAAKNIDELVEFYAWCKDVGIA 300
           +KESF +Y ++ + +G+L++ F E++  D ++ +DI    +K  +EL +FY+WCK++GIA
Sbjct: 241 VKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIA 300

Query: 301 GSSEYPEVQMITHNILITLGGFLKEMS--RRPKDYNETRME-----EEMKPLPVKELEYD 360
            SSEYPE++ IT   L  +  F+++ S     K     + E     +E +   V E + D
Sbjct: 301 RSSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQED 360

Query: 361 MNEIKTL---PPLE--NCVPVMQS-----VLRTQSRKEDLVNL-REDGVSADEQGNKLAL 420
           MN IK L   PP E  +  P  ++     + + Q    DL++L   +G  A + G+ LAL
Sbjct: 361 MNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDSLAL 420

Query: 421 ALFSGPPITNPNGSWETFSSNHEPDASSVWQTPVAEFGKPDWELALVETASHLSKQKPDM 480
           ALF GP           ++S    ++   W+    +    DWE ALV+TA++LS QK ++
Sbjct: 421 ALFDGP-----------YASGSGSESGPGWE--AFKDDSADWETALVQTATNLSGQKSEL 480

Query: 481 GGCFHPLLLNGMYDQEMVRRHVNVE-QFGGSSSSVAVALQGQGN-AAALLALPAPD---- 540
           GG F  LLLNGMY    V   V     +G S S+ ++A    G  AA +LALPAP     
Sbjct: 481 GGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPSTANG 540

Query: 541 --GMVEPIRYRDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWKQYGRDGMQGQVGL 554
             G +      DPFAASL V PP+YVQ+ +MEKKQ++LM+EQ++W QY RDG QG + L
Sbjct: 541 NAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGHMNL 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879765.14.4e-30089.19putative clathrin assembly protein At2g25430 [Benincasa hispida][more]
XP_008443967.11.1e-27185.79PREDICTED: putative clathrin assembly protein At2g25430 [Cucumis melo] >TYK06390... [more]
XP_023529315.16.9e-25376.90putative clathrin assembly protein At2g25430 [Cucurbita pepo subsp. pepo][more]
XP_022934011.19.0e-25376.69probable clathrin assembly protein At4g32285 [Cucurbita moschata][more]
XP_022145098.14.5e-25276.48probable clathrin assembly protein At4g32285 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q8S9J82.1e-16451.02Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LF202.6e-16250.84Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX471.7e-10840.03Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SA651.1e-10739.73Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LVD88.1e-6331.56Putative clathrin assembly protein At5g57200 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A5D3C4X95.5e-27285.79Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B9925.5e-27285.79putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1F0M34.4e-25376.69probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1CV162.2e-25276.48probable clathrin assembly protein At4g32285 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1I3H31.2e-25076.57probable clathrin assembly protein At4g32285 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G32285.11.5e-16551.02ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.21.5e-16551.02ENTH/ANTH/VHS superfamily protein [more]
AT2G25430.11.9e-16350.84epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p... [more]
AT4G02650.11.2e-10940.03ENTH/ANTH/VHS superfamily protein [more]
AT1G03050.17.7e-10939.73ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 30..161
e-value: 1.6E-29
score: 114.1
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 24..161
score: 28.00539
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 31..304
e-value: 4.7E-71
score: 239.0
IPR014712ANTH domain superfamilyGENE3D1.20.58.150ANTH domaincoord: 168..321
e-value: 2.7E-32
score: 113.4
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 3..155
e-value: 2.5E-36
score: 126.6
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 31..152
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 1..564
NoneNo IPR availablePANTHERPTHR22951:SF62ASSEMBLY PLANT-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 1..564
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 32..152
e-value: 6.39892E-55
score: 179.742
NoneNo IPR availableSUPERFAMILY89009GAT-like domaincoord: 174..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc06G12270.1Clc06G12270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048268 clathrin coat assembly
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0006900 vesicle budding from membrane
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0030276 clathrin binding
molecular_function GO:0005543 phospholipid binding