Clc06G04520 (gene) Watermelon (cordophanus) v2

Overview
NameClc06G04520
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionPectin lyase-like superfamily protein
LocationClcChr06: 4693722 .. 4696427 (-)
RNA-Seq ExpressionClc06G04520
SyntenyClc06G04520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGAGTTCCTTCCTTAAAGCTTATTCTTTAAAACTCAAGTCTCTATCTTTCTCCCATTACTTCTTGTTTCTGATAGTGCAGTTTTTGTCACTTTTCATTGATACATAATCCCAATCTTCACTGCTAAGCCAACCCATTTGCCTCATTTTCTGTTTAAGTTTAGCTGATAAGTTCCATGGAATTCAATGGACTTCTCATTGTAATGGTTTGGATTATAGCCATTTTGTTGGTGGAAAATTCAAGCAATGTAAATGGAAGATACCATTTTCATAAGGGGAAAAAGGAAGAGAAGCCTGATGAAGATTCCCCTGTTTATTCACCACCAGAAAAGGCAGTTCCTTCAAGTCCAGCTCAGCCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAAACTCCACTTCTGATTGTGTGTTTGATGTTACTGAGTTTGGAGCAGTTGGAGATGGCTGTACTGATGATACTGCCGCCTTTAAGGCAGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCCACTCTTTTGGTTCCTTCTAATCTTTGCTTTAAAATTACTTCAACCATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTCAGCAAATTCTACTTAAATTTTGAATTTCTCTTGGGGAGCTTGTTAAGTTTGTCGTGTGTCATCTTGTGATTAAACATTTAGTAGGTTGCACAAAGATAGGTGCAGTCTGAGATATAACTAAGTATTTTCATATTTGAAATGATTAGAAATTCAGTTGGAACTTATAAGTGAAAATATAACAAATCAGGTGGATGGGACCTTGATGCCACCGGAAGGACCGGAGTTGTGGCCGAAAGCGGATAGTCCGAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACAATGACTGGAGCTGGAACCATTGAAGGAAATGGCCAGAAATGGTGGGAGTTGCCATGCAAACCACACAGGGTACCTACATTGTATTTATTTTCACTTCATCATTTCCTTTGTTCTTGAATATCCTTCTTTCTTTCAGTTTCAACTTTCAATGCAAGGCTTAAATTTTTCCTTTGTTCACTTTTTCAGGGTCCAAATGGGACAACTCTCCCAGGACCATGTGACAGCCCTGCGGTAAGAAGGAAACACATATATTAATACTACTATATATGAATCAAAAATGCTGACTGAATTTTGATATTTGGGTTTTTTCAGTTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTGAATAGCTTGAGAATCCAAAACGGTCCACAGTTCCATATCAAATTTGATGGGTGTCAAGGAGTTCTAATAGAAAAGCTGTCAATTTCTTCCCCAAAGCTCAGTCCCAACACTGATGGAATTCACATAGAGAATACCAAAGGGGTTGAAATATACAACTCCATGATATCCAATGGTATGCCCACTGATTTCCAATTGTTATGCAATCAAAAATTAAATAAACAGAAAACATGAACATTAGAGAAATAGACACAGAAACTTACATGATTCACTAACAGAGAAATATTAACCTATTAAGTGTGTTTAACTTAACATCAAGTTAAATATTACAAAGACGCCAATATAGTTAGAGTAGTCTTAAGATTAAAATTGTAAATAACATAAATACGATTACACTAAAAATTGTAAATAACATAAATACGATTACACTAATACAAGAGACTATTTGCTGATTTATGATTTGCAAATATAGGTGATGACTGCATTTCAATTGGGCCGGGCTGTGCCAATGTGGCCATTGAAGGGGTTACTTGTGGGCCCAGTCATGGAATCAGGTACCAACAATGTCAATTCAATTCCTCTTCAATGTGAAAAATGCAATCTGCAATTATTTGTTTAACTCCAGTCCAACAGAGAGTAATCAGTTGTTGTATGTTTGGTTTTGGAAAAGAGCAGCATTGGGAGCCTGGGAGTCCACAATTCTCAGGCATGTGTTTCCAACATAACAGTAAAAAATGCAGTGATTAGAGACTCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGATCAGTAAGCAACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTGTCCAAGGATTGTCGGAACCAGACGTCAGCTGTGTTCGTGAACCAAGTGTTGTACAGGAACATCAAGGGAACTTACGACGTGAGGAACACTCCAATTCACTTCGCTTGCAGCGACACGGTAGCCTGCACAAACATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAGGGGGAGTTGGTAGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCAGAAACTTCTGAATATAGCTGCTAAAAGTGTTCTAATGCAATAATAAGGATAATAATACTGTATACAGTACCCACAGCAGAATCACCAAAGAACCATTTTTCTGTTAATGGGTCTTTGCTTGTTATGGCTTAGGCTCCAACTCCATCCCTTTCTGGGTTCATTTTTAGTCCTCCCATATTGAGTGATGTGATACCCTATTGTAAAATGTAGAATAGAGATGCGGTGCTCTCGGTTTTGCCCCTTCTAGTATGTATCTTCTAATTTATACAACTAAATTTACCTTTATGTTGGAGTTTTTTTTTTTTTTTTTTTTT

mRNA sequence

TTTGAGTTCCTTCCTTAAAGCTTATTCTTTAAAACTCAAGTCTCTATCTTTCTCCCATTACTTCTTGTTTCTGATAGTGCAGTTTTTGTCACTTTTCATTGATACATAATCCCAATCTTCACTGCTAAGCCAACCCATTTGCCTCATTTTCTGTTTAAGTTTAGCTGATAAGTTCCATGGAATTCAATGGACTTCTCATTGTAATGGTTTGGATTATAGCCATTTTGTTGGTGGAAAATTCAAGCAATGTAAATGGAAGATACCATTTTCATAAGGGGAAAAAGGAAGAGAAGCCTGATGAAGATTCCCCTGTTTATTCACCACCAGAAAAGGCAGTTCCTTCAAGTCCAGCTCAGCCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAAACTCCACTTCTGATTGTGTGTTTGATGTTACTGAGTTTGGAGCAGTTGGAGATGGCTGTACTGATGATACTGCCGCCTTTAAGGCAGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCCACTCTTTTGGTTCCTTCTAATCTTTGCTTTAAAATTACTTCAACCATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTGGATGGGACCTTGATGCCACCGGAAGGACCGGAGTTGTGGCCGAAAGCGGATAGTCCGAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACAATGACTGGAGCTGGAACCATTGAAGGAAATGGCCAGAAATGGTGGGAGTTGCCATGCAAACCACACAGGGGTCCAAATGGGACAACTCTCCCAGGACCATGTGACAGCCCTGCGTTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTGAATAGCTTGAGAATCCAAAACGGTCCACAGTTCCATATCAAATTTGATGGGTGTCAAGGAGTTCTAATAGAAAAGCTGTCAATTTCTTCCCCAAAGCTCAGTCCCAACACTGATGGAATTCACATAGAGAATACCAAAGGGGTTGAAATATACAACTCCATGATATCCAATGGTGATGACTGCATTTCAATTGGGCCGGGCTGTGCCAATGTGGCCATTGAAGGGGTTACTTGTGGGCCCAGTCATGGAATCAGCAGCATTGGGAGCCTGGGAGTCCACAATTCTCAGGCATGTGTTTCCAACATAACAGTAAAAAATGCAGTGATTAGAGACTCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGATCAGTAAGCAACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTGTCCAAGGATTGTCGGAACCAGACGTCAGCTGTGTTCGTGAACCAAGTGTTGTACAGGAACATCAAGGGAACTTACGACGTGAGGAACACTCCAATTCACTTCGCTTGCAGCGACACGGTAGCCTGCACAAACATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAGGGGGAGTTGGTAGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCAGAAACTTCTGAATATAGCTGCTAAAAGTGTTCTAATGCAATAATAAGGATAATAATACTGTATACAGTACCCACAGCAGAATCACCAAAGAACCATTTTTCTGTTAATGGGTCTTTGCTTGTTATGGCTTAGGCTCCAACTCCATCCCTTTCTGGGTTCATTTTTAGTCCTCCCATATTGAGTGATGTGATACCCTATTGTAAAATGTAGAATAGAGATGCGGTGCTCTCGGTTTTGCCCCTTCTAGTATGTATCTTCTAATTTATACAACTAAATTTACCTTTATGTTGGAGTTTTTTTTTTTTTTTTTTTTT

Coding sequence (CDS)

ATGGAATTCAATGGACTTCTCATTGTAATGGTTTGGATTATAGCCATTTTGTTGGTGGAAAATTCAAGCAATGTAAATGGAAGATACCATTTTCATAAGGGGAAAAAGGAAGAGAAGCCTGATGAAGATTCCCCTGTTTATTCACCACCAGAAAAGGCAGTTCCTTCAAGTCCAGCTCAGCCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAAACTCCACTTCTGATTGTGTGTTTGATGTTACTGAGTTTGGAGCAGTTGGAGATGGCTGTACTGATGATACTGCCGCCTTTAAGGCAGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCCACTCTTTTGGTTCCTTCTAATCTTTGCTTTAAAATTACTTCAACCATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTGGATGGGACCTTGATGCCACCGGAAGGACCGGAGTTGTGGCCGAAAGCGGATAGTCCGAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACAATGACTGGAGCTGGAACCATTGAAGGAAATGGCCAGAAATGGTGGGAGTTGCCATGCAAACCACACAGGGGTCCAAATGGGACAACTCTCCCAGGACCATGTGACAGCCCTGCGTTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTGAATAGCTTGAGAATCCAAAACGGTCCACAGTTCCATATCAAATTTGATGGGTGTCAAGGAGTTCTAATAGAAAAGCTGTCAATTTCTTCCCCAAAGCTCAGTCCCAACACTGATGGAATTCACATAGAGAATACCAAAGGGGTTGAAATATACAACTCCATGATATCCAATGGTGATGACTGCATTTCAATTGGGCCGGGCTGTGCCAATGTGGCCATTGAAGGGGTTACTTGTGGGCCCAGTCATGGAATCAGCAGCATTGGGAGCCTGGGAGTCCACAATTCTCAGGCATGTGTTTCCAACATAACAGTAAAAAATGCAGTGATTAGAGACTCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGATCAGTAAGCAACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTGTCCAAGGATTGTCGGAACCAGACGTCAGCTGTGTTCGTGAACCAAGTGTTGTACAGGAACATCAAGGGAACTTACGACGTGAGGAACACTCCAATTCACTTCGCTTGCAGCGACACGGTAGCCTGCACAAACATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAGGGGGAGTTGGTAGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCAGAAACTTCTGAATATAGCTGCTAA

Protein sequence

MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDEDSPVYSPPEKAVPSSPAQPVPSVPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFHIKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Homology
BLAST of Clc06G04520 vs. NCBI nr
Match: XP_038906648.1 (polygalacturonase At1g48100 [Benincasa hispida])

HSP 1 Score: 939.5 bits (2427), Expect = 1.1e-269
Identity = 451/479 (94.15%), Postives = 467/479 (97.49%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDEDSPVYS-PPEKAVPSSPA 60
           MEFNG+ IVM+WI+A LLVENSSNVNGRYHFHKGKKEEKP+EDSPV+S PPEKAVPSSPA
Sbjct: 1   MEFNGVFIVMIWIVATLLVENSSNVNGRYHFHKGKKEEKPNEDSPVFSPPPEKAVPSSPA 60

Query: 61  QPVPSVPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLLV 120
           +PVPSVPSDPYPN+PGNS+SDCVFDVT+FGAVGDGC DDTAAFKAAWKAACAVESATLLV
Sbjct: 61  EPVPSVPSDPYPNEPGNSSSDCVFDVTDFGAVGDGCADDTAAFKAAWKAACAVESATLLV 120

Query: 121 PSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTG 180
           PS LCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPE WPKADSPRQWLVFYRLDQMT+TG
Sbjct: 121 PSGLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPESWPKADSPRQWLVFYRLDQMTLTG 180

Query: 181 AGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFHI 240
            GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAVNSLRIQN P FH+
Sbjct: 181 TGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHM 240

Query: 241 KFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAI 300
           KFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGV IYNSMISNGDDCISIGPGCANVAI
Sbjct: 241 KFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAI 300

Query: 301 EGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILF 360
           +GVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIRDSDNGVRIKTWQGGSGSVS+ILF
Sbjct: 301 DGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTWQGGSGSVSDILF 360

Query: 361 ENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTV 420
           ENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLY+NIKGTYDVRNTPIHFACSDTV
Sbjct: 361 ENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTV 420

Query: 421 ACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 479
           ACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 478

BLAST of Clc06G04520 vs. NCBI nr
Match: XP_008458305.1 (PREDICTED: polygalacturonase At1g48100 [Cucumis melo] >TYK02978.1 polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 921.8 bits (2381), Expect = 2.5e-264
Identity = 444/480 (92.50%), Postives = 464/480 (96.67%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDEDSPVYSPPEKAVPSSPAQ 60
           MEFNG+LI+M+W+IAIL VENSSNV+GRYHFHKGKK    DEDSPV SPPEKAVPS+PAQ
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKK----DEDSPVSSPPEKAVPSTPAQ 60

Query: 61  PVPSVPSDPYPNDP--GNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLL 120
            VPSVPSDPYPNDP  GNSTSDCVFDVT+FGAVGDGCTDDTAAFKAAWKAACAVESATLL
Sbjct: 61  SVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLL 120

Query: 121 VPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMT 180
           VPSNLCFKITSTIFSGPCKPGLVFKVDGTL PP+GPE WPKADSPRQWLVFYRLDQMT+T
Sbjct: 121 VPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLT 180

Query: 181 GAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFH 240
           G+GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAVNSLRIQN P FH
Sbjct: 181 GSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFH 240

Query: 241 IKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVA 300
           +KFDGC+GVLIE+LSISSPKLSPNTDGIHIENTKGV IYNSMISNGDDCISIGPGCANVA
Sbjct: 241 MKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVA 300

Query: 301 IEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNIL 360
           IEGVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIRDSDNG+RIKTWQGGSGSVS+IL
Sbjct: 301 IEGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 360

Query: 361 FENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDT 420
           FENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLY+NIKGTYDVRNTPIHFACSD+
Sbjct: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 420

Query: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 479
           VACTNITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

BLAST of Clc06G04520 vs. NCBI nr
Match: XP_004138524.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45612.2 hypothetical protein Csa_005406 [Cucumis sativus])

HSP 1 Score: 921.4 bits (2380), Expect = 3.2e-264
Identity = 444/480 (92.50%), Postives = 462/480 (96.25%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDEDSPVYSPPEKAVPSSPAQ 60
           ME NG+ IVM+W++AIL VENSSNVNGRYHFHKGKK    DEDS V SPPE AVPS+PAQ
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKK----DEDSSVSSPPENAVPSTPAQ 60

Query: 61  PVPSVPSDPYPNDP--GNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLL 120
           P+PSVPSDPYPNDP  GNSTSDCVFDVT+FGAVGDGCTDDTAAFKAAWKAACAVESATLL
Sbjct: 61  PLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLL 120

Query: 121 VPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMT 180
           VPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPP+GPE WPKADSPRQWLVFYRLDQMT+T
Sbjct: 121 VPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLT 180

Query: 181 GAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFH 240
           G+GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAVNSLRIQN P FH
Sbjct: 181 GSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFH 240

Query: 241 IKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVA 300
           +KFDGC+GVLIEKLSISSPKLSPNTDGIHIENTKGV IYNSMISNGDDCISIGPGCANVA
Sbjct: 241 MKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVA 300

Query: 301 IEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNIL 360
           IEGVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIRDSDNG+RIKTWQGGSGSVS+IL
Sbjct: 301 IEGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 360

Query: 361 FENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDT 420
           FENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLY+NIKGTYDVRNTPIHFACSD+
Sbjct: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 420

Query: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 479
           VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 475

BLAST of Clc06G04520 vs. NCBI nr
Match: KAG7024752.1 (Polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 891.0 bits (2301), Expect = 4.7e-255
Identity = 427/483 (88.41%), Postives = 452/483 (93.58%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDED----SPVYS-PPEKAVP 60
           MEFN +  VM+WI AIL+ EN SNV GRYHFHKG+K++ PDE     SPVYS PPE +VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  SSPAQPVPSVPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESA 120
           S+PA P P VPSDPYP+DPGN+TSDCVFDVT +GAVGDGC DDTAAF+AAWKAACAVESA
Sbjct: 61  STPADPAPPVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQM 180
           TLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTLMPP+GPE WPKADS RQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TMTGAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGP 240
           T+TG GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAV+ LRIQN P
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHIKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCA 300
           QFH+KFDGC+GVLI+KLSISSPKLSPNTDGIHIENTKGV IYNSMI NGDDCISIGPGCA
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 NVAIEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVS 360
           NV IEGVTCGPSHGISSIGSLGVHNSQACVSNITV+NAVIR+SDNGVRIKTWQGGSG+VS
Sbjct: 301 NVDIEGVTCGPSHGISSIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVS 360

Query: 361 NILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFAC 420
           +ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLY+NIKGTYDVRNTPIHFAC
Sbjct: 361 DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC 420

Query: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSE 479
           SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET E
Sbjct: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE 480

BLAST of Clc06G04520 vs. NCBI nr
Match: XP_022930280.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 888.6 bits (2295), Expect = 2.3e-254
Identity = 428/483 (88.61%), Postives = 452/483 (93.58%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDED----SPVYS-PPEKAVP 60
           MEFN +  VM+WI AIL+ EN SNV GRYHFHKG+K++ PDE     SPVYS PPE +VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  SSPAQPVPSVPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESA 120
           S+PA P PSVPSDPYP+DPGN+TSDCVFDVT +GAVGDGC DDTAAF+AAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQM 180
           TLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTLMPP+GPE WPKADS RQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLDQM 180

Query: 181 TMTGAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGP 240
           T+TG GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAV+ LRIQN P
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHIKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCA 300
           QFH+KFDGC+GVLIEKLSISSPKLSPNTDGIHIENTKGV IYNSMI NGDDCISIGPGCA
Sbjct: 241 QFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 NVAIEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVS 360
           NV IEGVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIR+SDNGVRIKTWQGGSG+VS
Sbjct: 301 NVDIEGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVS 360

Query: 361 NILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFAC 420
           +ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLY+NIKGTYDVRNTPIHFAC
Sbjct: 361 DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC 420

Query: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSE 479
           SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET E
Sbjct: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE 480

BLAST of Clc06G04520 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 1.7e-196
Identity = 336/476 (70.59%), Postives = 385/476 (80.88%), Query Frame = 0

Query: 8   IVMVWIIAILLVENSSNV-NGRYHFHKGKKEEKPDEDSPVYSPPEKAVPSSPAQPVPSVP 67
           I++++II+ +L  + +NV   RYH+HKG+         P+  PP    P   A P   VP
Sbjct: 7   ILVIYIISTVLFGHFTNVARARYHYHKGRH----GVTHPL--PPPPPPPLETANPPDQVP 66

Query: 68  SDPYPND---PGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNL 127
           SDPYP+    PG+S S CVFDVT FGAVGDG  DDTAAF+ AWKAACAVES  +L P   
Sbjct: 67  SDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGG 126

Query: 128 CFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAGTI 187
            FKITSTIFSGPCKPGLVF++DG LMPP+GPE WP+ D+  QWLVFYRLD  T +G GT+
Sbjct: 127 VFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTV 186

Query: 188 EGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFHIKFDG 247
           EGNGQKWW+LPCKPHRGP+G++  GPC SP +IRFFMS+N+ V  LRIQN PQFH+KFDG
Sbjct: 187 EGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDG 246

Query: 248 CQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAIEGVT 307
           CQGVLI ++ ISSPKLSPNTDGIH+ NT+ V IYNS++SNGDDCISIG GC++V I+GVT
Sbjct: 247 CQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVT 306

Query: 308 CGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILFENIQ 367
           CGPSHGI SIGSLGVHNSQACVSNITV+N VIRDSDNG+R+KTWQGG+GSVSN+LFENIQ
Sbjct: 307 CGPSHGI-SIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQ 366

Query: 368 MENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTN 427
           MENV NCIIVDQYYC SKDCRN+TSAV V  V YRNIKGTYDVR+ PIHFACSDTVACTN
Sbjct: 367 MENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTN 426

Query: 428 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP-QNVAETSEYSC 479
           ITMSEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G P    A  S Y C
Sbjct: 427 ITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of Clc06G04520 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 3.3e-86
Identity = 168/389 (43.19%), Postives = 230/389 (59.13%), Query Frame = 0

Query: 79  SDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESAT-LLVPSNLCFKITSTIFSGPCK 138
           S+ +  V  FGA G+G TDDT AF  AWK AC+ +  T +LVP N    +     SGPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 139 PGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAGTIEGNGQKWWELPCKP 198
             L  ++ GT++ P  P++W +  + R+WL F+ L ++T+ G GT+ G GQ+WWE  CK 
Sbjct: 109 ARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK- 168

Query: 199 HRGPNGTTLPGPC-DSPALIRFFMSSNLAVNSLRIQNGPQFHIKFDGCQGVLIEKLSISS 258
           H   N      PC  +P  + F    N+ V +L + +  Q HI    C+ V I  L + +
Sbjct: 169 HNHSN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIA 228

Query: 259 PKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISSIGSL 318
           P  SPNTDGIHI  ++G+ I N+ +S GDDCISI      ++I  + CGP HGI SIGSL
Sbjct: 229 PATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGI-SIGSL 288

Query: 319 GVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILFENIQMENVRNCIIVDQY 378
           G   S   V +ITV  A+I D+ NGVRIKTWQGGSG VS I+F NI+M NV N II+DQY
Sbjct: 289 GKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQY 348

Query: 379 YCLS-KDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHE 438
           YC S K C NQTSA+ +  + + +++GT       I  +CSD+  C NI + +++L P  
Sbjct: 349 YCDSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSN 408

Query: 439 GELVEDPFCWNAFGIQETLTIPPIDCLQE 465
           G+   + FCW A+G       PP  CL +
Sbjct: 409 GDGFTESFCWEAYGSSSGQVYPP-PCLSD 426

BLAST of Clc06G04520 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.6e-83
Identity = 158/358 (44.13%), Postives = 221/358 (61.73%), Query Frame = 0

Query: 82  VFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLV 141
           +F+V ++GAVGDG  D T AF  AW+AAC   SA LLVP N  F + +  F+GPC+P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 142 FKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAGTIEGNGQKWWELPCKPHRGP 201
           FKVDG +   + P  W    + R WL F +L   T+ G G I+G G++WW   CK     
Sbjct: 118 FKVDGIIAAYQNPASW---KNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCK---WV 177

Query: 202 NGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFHIKFDGCQGVLIEKLSISSPKLSP 261
           NG  +    D P  I+F  S+ L +  L++ N P+FH+ F  C+GV I  +SI++P+ SP
Sbjct: 178 NGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSP 237

Query: 262 NTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISSIGSLGVHNS 321
           NTDGI I  +K   +  + I  GDDC++IG G +N+ IE + CGP HGI SIGSLG  NS
Sbjct: 238 NTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGI-SIGSLGRENS 297

Query: 322 QACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILFENIQMENVRNCIIVDQYYCLSK 381
           +A VS + V  A   D+ NG+RIKTWQGGSG  S+I++EN++M N  N I+++Q+YC S 
Sbjct: 298 RAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSA 357

Query: 382 D-CRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGEL 439
             C+NQ SAV +  V Y+NI+GT       I   CSD++ C +I +S++ L    G++
Sbjct: 358 SACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

BLAST of Clc06G04520 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 8.4e-82
Identity = 161/351 (45.87%), Postives = 213/351 (60.68%), Query Frame = 0

Query: 82  VFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLV 141
           VF+V ++GAVGDG  D T AF   W AAC   SA LLVP+N  F + + +F GPC+P L 
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLS 117

Query: 142 FKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAGTIEGNGQKWWELPCKPHRGP 201
           FKVDGT++    P  W  +   + WL F +L    + G G I+G GQ+WW   CK     
Sbjct: 118 FKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV---V 177

Query: 202 NGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFHIKFDGCQGVLIEKLSISSPKLSP 261
           NG T+    + P  I+   S ++ V  L + N P+FH+ F  C+GV I+ L I +P+ SP
Sbjct: 178 NGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSP 237

Query: 262 NTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISSIGSLGVHNS 321
           NTDGI I  +K   I   +I  GDDCI+IG G +N+ I+ + CGP HGI SIGSLG  NS
Sbjct: 238 NTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGI-SIGSLGRDNS 297

Query: 322 QACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILFENIQMENVRNCIIVDQYYCLSK 381
           +A VS++ V  A   D+ NG+RIKTWQGGSG  S I +EN++M N  N I+++Q+YC S 
Sbjct: 298 RAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSA 357

Query: 382 D-CRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTNITMSEVEL 432
             C+NQ SAV +  V Y+NI GT       I   CSD+V CT I +S V L
Sbjct: 358 SACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSL 400

BLAST of Clc06G04520 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 5.4e-81
Identity = 167/384 (43.49%), Postives = 227/384 (59.11%), Query Frame = 0

Query: 84  DVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFK 143
           +V +FGA GDG  DDT AF+ AWKAAC+  S+ +L+     + +    FSGPCK GL  +
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTMQ 150

Query: 144 VDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAGTIEGNGQKWWELPCKPHRGPNG 203
           + GT+   +    + K    R WLVF  +  + + G GTI GNG+ WW+  CK ++    
Sbjct: 151 IYGTIEASDDRSDYRK--DGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL-- 210

Query: 204 TTLPGPC-DSPALIRFFMSSNLAVNSLRIQNGPQFHIKFDGCQGVLIEKLSISSPKLSPN 263
                PC D+P  + F+ S ++ V +L+I+N  Q H+ FD C  V    L +++P+ SPN
Sbjct: 211 -----PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPN 270

Query: 264 TDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISSIGSLGVHNSQ 323
           TDGIH+  T+ + I + +I  GDDCISI  G   V +  +TCGP HGI SIGSLG  NS+
Sbjct: 271 TDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGI-SIGSLGYGNSE 330

Query: 324 ACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILFENIQMENVRNCIIVDQYYC-LSK 383
           A VS++ V  A +  + NGVRIKTWQGGSGS SNI F+N++M NV N II+DQ YC   K
Sbjct: 331 AHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDK 390

Query: 384 DCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED 443
            C+ Q+SAV V  V+Y+NIKGT    N  I F CS    C  I + +V+ L  EG     
Sbjct: 391 PCQEQSSAVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAK 450

Query: 444 PFCWNAFGIQETLTIPPIDCLQEG 466
             C N   + ET  + P  C +EG
Sbjct: 451 ALC-NNVELSETGVVSP-HCQEEG 459

BLAST of Clc06G04520 vs. ExPASy TrEMBL
Match: A0A5D3BVF7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00640 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.2e-264
Identity = 444/480 (92.50%), Postives = 464/480 (96.67%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDEDSPVYSPPEKAVPSSPAQ 60
           MEFNG+LI+M+W+IAIL VENSSNV+GRYHFHKGKK    DEDSPV SPPEKAVPS+PAQ
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKK----DEDSPVSSPPEKAVPSTPAQ 60

Query: 61  PVPSVPSDPYPNDP--GNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLL 120
            VPSVPSDPYPNDP  GNSTSDCVFDVT+FGAVGDGCTDDTAAFKAAWKAACAVESATLL
Sbjct: 61  SVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLL 120

Query: 121 VPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMT 180
           VPSNLCFKITSTIFSGPCKPGLVFKVDGTL PP+GPE WPKADSPRQWLVFYRLDQMT+T
Sbjct: 121 VPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLT 180

Query: 181 GAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFH 240
           G+GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAVNSLRIQN P FH
Sbjct: 181 GSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFH 240

Query: 241 IKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVA 300
           +KFDGC+GVLIE+LSISSPKLSPNTDGIHIENTKGV IYNSMISNGDDCISIGPGCANVA
Sbjct: 241 MKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVA 300

Query: 301 IEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNIL 360
           IEGVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIRDSDNG+RIKTWQGGSGSVS+IL
Sbjct: 301 IEGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 360

Query: 361 FENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDT 420
           FENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLY+NIKGTYDVRNTPIHFACSD+
Sbjct: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 420

Query: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 479
           VACTNITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

BLAST of Clc06G04520 vs. ExPASy TrEMBL
Match: A0A1S3C7J5 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103497761 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.2e-264
Identity = 444/480 (92.50%), Postives = 464/480 (96.67%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDEDSPVYSPPEKAVPSSPAQ 60
           MEFNG+LI+M+W+IAIL VENSSNV+GRYHFHKGKK    DEDSPV SPPEKAVPS+PAQ
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKK----DEDSPVSSPPEKAVPSTPAQ 60

Query: 61  PVPSVPSDPYPNDP--GNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLL 120
            VPSVPSDPYPNDP  GNSTSDCVFDVT+FGAVGDGCTDDTAAFKAAWKAACAVESATLL
Sbjct: 61  SVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLL 120

Query: 121 VPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMT 180
           VPSNLCFKITSTIFSGPCKPGLVFKVDGTL PP+GPE WPKADSPRQWLVFYRLDQMT+T
Sbjct: 121 VPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLT 180

Query: 181 GAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFH 240
           G+GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAVNSLRIQN P FH
Sbjct: 181 GSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFH 240

Query: 241 IKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVA 300
           +KFDGC+GVLIE+LSISSPKLSPNTDGIHIENTKGV IYNSMISNGDDCISIGPGCANVA
Sbjct: 241 MKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVA 300

Query: 301 IEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNIL 360
           IEGVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIRDSDNG+RIKTWQGGSGSVS+IL
Sbjct: 301 IEGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 360

Query: 361 FENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDT 420
           FENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLY+NIKGTYDVRNTPIHFACSD+
Sbjct: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 420

Query: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 479
           VACTNITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

BLAST of Clc06G04520 vs. ExPASy TrEMBL
Match: A0A6J1EWI8 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 428/483 (88.61%), Postives = 452/483 (93.58%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDED----SPVYS-PPEKAVP 60
           MEFN +  VM+WI AIL+ EN SNV GRYHFHKG+K++ PDE     SPVYS PPE +VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  SSPAQPVPSVPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESA 120
           S+PA P PSVPSDPYP+DPGN+TSDCVFDVT +GAVGDGC DDTAAF+AAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQM 180
           TLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTLMPP+GPE WPKADS RQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLDQM 180

Query: 181 TMTGAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGP 240
           T+TG GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAV+ LRIQN P
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHIKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCA 300
           QFH+KFDGC+GVLIEKLSISSPKLSPNTDGIHIENTKGV IYNSMI NGDDCISIGPGCA
Sbjct: 241 QFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 NVAIEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVS 360
           NV IEGVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIR+SDNGVRIKTWQGGSG+VS
Sbjct: 301 NVDIEGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVS 360

Query: 361 NILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFAC 420
           +ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLY+NIKGTYDVRNTPIHFAC
Sbjct: 361 DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC 420

Query: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSE 479
           SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET E
Sbjct: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE 480

BLAST of Clc06G04520 vs. ExPASy TrEMBL
Match: A0A6J1CB48 (polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009879 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.3e-254
Identity = 433/491 (88.19%), Postives = 458/491 (93.28%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDED----SPVYS-PPEKAVP 60
           MEFN +L ++V I AILLVENSSNV GRYHFHKG+K++KPDED    SPVYS PPE+AVP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  SS--------PAQPVPSVPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWK 120
           SS        PA+P PSVPSDPYP+DP NSTSDCVFDVT++GAVGDGCTDDTAAF+AAWK
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDPRNSTSDCVFDVTDYGAVGDGCTDDTAAFRAAWK 120

Query: 121 AACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWL 180
           AACAVESAT+LVPS+ CF ITSTIFSGPCKPGLVFKVDG+LMPP+GPE WPKADS RQWL
Sbjct: 121 AACAVESATILVPSDHCFIITSTIFSGPCKPGLVFKVDGSLMPPDGPESWPKADSVRQWL 180

Query: 181 VFYRLDQMTMTGAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVN 240
           VFYRLDQMTMTG GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNL V+
Sbjct: 181 VFYRLDQMTMTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLVVS 240

Query: 241 SLRIQNGPQFHIKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDC 300
            LRIQN PQFH+KFDGC+GVLIEKLSISSPKLSPNTDGIHIENTKGV IYNSMISNGDDC
Sbjct: 241 GLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDC 300

Query: 301 ISIGPGCANVAIEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTW 360
           ISIGPGCANV IEGVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIRDSDNGVRIKTW
Sbjct: 301 ISIGPGCANVDIEGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTW 360

Query: 361 QGGSGSVSNILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVR 420
           QGGSGSVS+ILFENIQMENVRNCIIVDQYYCLSK C NQTSAVFVNQVLYRNIKGTYDVR
Sbjct: 361 QGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKGCLNQTSAVFVNQVLYRNIKGTYDVR 420

Query: 421 NTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP 479
           NTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNA+GIQETLTIPPIDCLQ+G+P
Sbjct: 421 NTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAYGIQETLTIPPIDCLQDGQP 480

BLAST of Clc06G04520 vs. ExPASy TrEMBL
Match: A0A6J1KH63 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=3 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.4e-252
Identity = 424/483 (87.78%), Postives = 450/483 (93.17%), Query Frame = 0

Query: 1   MEFNGLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDED----SPVYS-PPEKAVP 60
           MEFN +  VM+WI AIL+ EN SNV GRYHFHKG+K++ PDE     SPVYS PPE + P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  SSPAQPVPSVPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESA 120
           S+PA   PSVPSDPYP+DPGN+TSDCVFDVT +GAVGDGC DDTAAF+AAWKAACAVESA
Sbjct: 61  STPADQAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQM 180
           TLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTLMPP+GPE WPKADS RQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TMTGAGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGP 240
           T+TG GTIEGNGQKWWELPCKPHRGPNG+TLPGPCDSPALIRFFMSSNLAV+ LRIQN P
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHIKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCA 300
           QFH+KFDGC+GVLI+KLSISSPKLSPNTDGIHIENTKGV IYNSMI NGDDCISIGPGCA
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 NVAIEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVS 360
           NV IEGVTCGPSHGI SIGSLGVHNSQACVSNITV+NAVIR+SDNGVRIKTWQGGSG+VS
Sbjct: 301 NVDIEGVTCGPSHGI-SIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVS 360

Query: 361 NILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFAC 420
           +ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQ+LY+NIKGTYDVRNTPIHFAC
Sbjct: 361 DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFAC 420

Query: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSE 479
           SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET E
Sbjct: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE 480

BLAST of Clc06G04520 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 686.8 bits (1771), Expect = 1.2e-197
Identity = 336/476 (70.59%), Postives = 385/476 (80.88%), Query Frame = 0

Query: 8   IVMVWIIAILLVENSSNV-NGRYHFHKGKKEEKPDEDSPVYSPPEKAVPSSPAQPVPSVP 67
           I++++II+ +L  + +NV   RYH+HKG+         P+  PP    P   A P   VP
Sbjct: 7   ILVIYIISTVLFGHFTNVARARYHYHKGRH----GVTHPL--PPPPPPPLETANPPDQVP 66

Query: 68  SDPYPND---PGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNL 127
           SDPYP+    PG+S S CVFDVT FGAVGDG  DDTAAF+ AWKAACAVES  +L P   
Sbjct: 67  SDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGG 126

Query: 128 CFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAGTI 187
            FKITSTIFSGPCKPGLVF++DG LMPP+GPE WP+ D+  QWLVFYRLD  T +G GT+
Sbjct: 127 VFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTV 186

Query: 188 EGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFHIKFDG 247
           EGNGQKWW+LPCKPHRGP+G++  GPC SP +IRFFMS+N+ V  LRIQN PQFH+KFDG
Sbjct: 187 EGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDG 246

Query: 248 CQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAIEGVT 307
           CQGVLI ++ ISSPKLSPNTDGIH+ NT+ V IYNS++SNGDDCISIG GC++V I+GVT
Sbjct: 247 CQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVT 306

Query: 308 CGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILFENIQ 367
           CGPSHGI SIGSLGVHNSQACVSNITV+N VIRDSDNG+R+KTWQGG+GSVSN+LFENIQ
Sbjct: 307 CGPSHGI-SIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQ 366

Query: 368 MENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTN 427
           MENV NCIIVDQYYC SKDCRN+TSAV V  V YRNIKGTYDVR+ PIHFACSDTVACTN
Sbjct: 367 MENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTN 426

Query: 428 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP-QNVAETSEYSC 479
           ITMSEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G P    A  S Y C
Sbjct: 427 ITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of Clc06G04520 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 537.0 bits (1382), Expect = 1.6e-152
Identity = 261/471 (55.41%), Postives = 338/471 (71.76%), Query Frame = 0

Query: 5   GLLIVMVWIIAILLVENSSNVNGRYHFHKGKKEEKPDEDSPVYSPPEKAVPSSPAQPVPS 64
           G + +++ ++++    N  +     H +      KP   S   S P    P  P  P PS
Sbjct: 13  GFMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPS 72

Query: 65  VPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESAT---LLVPS 124
           +P  P P+D     ++ +++V ++GAVGDG TDDT AFK AW ++C  E+ T   LLVP 
Sbjct: 73  LP--PSPSDDPADDNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPY 132

Query: 125 NLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAG 184
              F I STIF+GPC+    F+VDGT++ P+GPE WP   S RQWLVFYR++ M + G G
Sbjct: 133 GYTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEG 192

Query: 185 TIEGNGQKWWELPCKPHRGPNGTTL-PGPCDSPALIRFFMSSNLAVNSLRIQNGPQFHIK 244
            I+G GQKWW+LPCKPHR  N + +  GPCDSP  +RFFMSSNL V  L+I+N PQFH +
Sbjct: 193 VIDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFR 252

Query: 245 FDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAIE 304
           FDGCQGV +E L I++P LSPNTDGIHIEN+  V IYNS+ISNGDDC+SIG G  +V I 
Sbjct: 253 FDGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIR 312

Query: 305 GVTCGP-SHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILF 364
            +TCGP  HGI SIGSLG HNS+ACVSNITV+++VI+ SDNGVRIKTWQGGSGSVS + F
Sbjct: 313 NLTCGPGGHGI-SIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTF 372

Query: 365 ENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTV 424
            NI +++VRN II+DQYYC++KDC N+TSAVFV+ + Y+ IKGTYD+R+ P+HF CSD V
Sbjct: 373 NNIHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAV 432

Query: 425 ACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNV 471
            CTN+T+S++ELLP +GE+V DPFCWNA+GI E L+IPP+ CL    P+ +
Sbjct: 433 PCTNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGL 480

BLAST of Clc06G04520 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.1e-144
Identity = 241/408 (59.07%), Postives = 303/408 (74.26%), Query Frame = 0

Query: 63  PSVPSDPYPNDPGNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAAC---AVESATLLV 122
           PS   +P  ND  N  +  VFDVT FGA+GD  TDDT+AFK AW AAC     +SA LLV
Sbjct: 29  PSPAPNPAYNDNDN-IAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLV 88

Query: 123 PSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTG 182
           P   CF +  T F+GPC+  LV ++DG ++ P+GP  WP ++  RQW++FYR++ +++ G
Sbjct: 89  PYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWP-SNYQRQWMMFYRVNGLSIQG 148

Query: 183 AGTIEGNGQKWWELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNSLRIQNGPQFHI 242
           +G I G GQKWW LPCKPH+G NGTT  GPCDSP  IR F SS + +  +   N  QFH+
Sbjct: 149 SGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHV 208

Query: 243 KFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISIGPGCANVAI 302
           +FD C  V+++ + I +P  SPNTDGIHIENT  V+I NSMISNGDDCISIG GC NV I
Sbjct: 209 RFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDI 268

Query: 303 EGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQGGSGSVSNILF 362
           + VTCGPSHGI SIGSLGVHNSQA VSNITV N+ I +SDNGVRIKTWQGGSGSVS I+F
Sbjct: 269 KNVTCGPSHGI-SIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVF 328

Query: 363 ENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTV 422
            NI M NVRN I++DQYYC + +C NQTSAV ++ VLY NIKGTYD+R+ PIHF CSD+V
Sbjct: 329 SNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSV 388

Query: 423 ACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP 468
            CTN+T++EV+L P +G+ +E+PFCWNA+G  + +T+PP+ CL +  P
Sbjct: 389 PCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of Clc06G04520 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 492.7 bits (1267), Expect = 3.4e-139
Identity = 234/414 (56.52%), Postives = 302/414 (72.95%), Query Frame = 0

Query: 63  PSVPSDPYPNDPGNSTS-------DCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVE-- 122
           P  PS P PNDP  S S       + V+DV ++GAVG+G  DDT +FK AW +AC+    
Sbjct: 39  PPSPS-PNPNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSACSNNKN 98

Query: 123 --SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYR 182
             ++ L VP    F I STIF+GPC+    F+VDGT++P +GP+ WP + + RQWL FYR
Sbjct: 99  NTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYR 158

Query: 183 LDQMTMTGAGTIEGNGQKWWELPCKPH-RGPNGTTLPGPCDSPALIRFFMSSNLAVNSLR 242
           ++ M + GAG I+G GQ WW+LPCKPH +  N T L GPC+SPA +RFFMSSN+ V  L 
Sbjct: 159 INGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVIVKGLS 218

Query: 243 IQNGPQFHIKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCISI 302
           I+N PQ H+K DGC  V I  L I SP  SPNTDGIHIEN+  VEIYNS+ISNGDDC+SI
Sbjct: 219 IKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGDDCVSI 278

Query: 303 GPGCANVAIEGVTCGP-SHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQG 362
           GPG  ++ I  +TCGP  HGI SIGSLG  NS ACVSN+TV+++ I+ S+NGVRIKTWQG
Sbjct: 279 GPGAYDIDIRNITCGPGGHGI-SIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQG 338

Query: 363 GSGSVSNILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRNT 422
           GSGSVS + F+NI ++ VRN II+DQYYC +K C N+TSAVFVN ++Y++IKGTYD+R+ 
Sbjct: 339 GSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRSP 398

Query: 423 PIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQ 464
           P+HF CS+ V CTN+T+S +ELLP + ++V  PFCWNA+GI +  ++P I CL+
Sbjct: 399 PMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLK 450

BLAST of Clc06G04520 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 364.0 bits (933), Expect = 1.8e-100
Identity = 201/480 (41.88%), Postives = 279/480 (58.13%), Query Frame = 0

Query: 23  SNVNGRYHF-HKGK-------KEEKPDEDSPVYSPPEKAVPSSPAQPVPSVPSDPYPNDP 82
           S+ N  +H  HK K       +  K ++ SPV S P +     P  P    P  P P  P
Sbjct: 59  SHENNHHHMSHKSKPKPKMKTQPPKSNDGSPVVSQPPQV--QQPPPPHVQPPPTPLPLQP 118

Query: 83  GNSTSDCVFDVTEFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSG 142
              +    F+V +FGA GDG +DDT AF+AAW +AC VE++T+++P +  F +    FSG
Sbjct: 119 VEDSQQ--FNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVGPISFSG 178

Query: 143 P-CKPGLVFKVDGTLMPPEGPELWPKADSPRQWLVFYRLDQMTMTGAGTIEGNGQKWW-- 202
           P C+  +VF+++G ++ P   E W        W+ F +L  +T+ G G I+G G  WW  
Sbjct: 179 PYCQANIVFQLEGMIVAPTDTESW--GGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQ 238

Query: 203 -----------------------ELPCKPHRGPNGTTLPGPCDSPALIRFFMSSNLAVNS 262
                                  E P  P R      +P     P  +RF+ S ++ V  
Sbjct: 239 DYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPS--IKPTALRFYGSIDVTVTG 298

Query: 263 LRIQNGPQFHIKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVEIYNSMISNGDDCI 322
           + IQN PQ H+KFD C  VL+  +++SSP  SPNTDGIH++NT+ V I+ + ++ GDDCI
Sbjct: 299 ITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCI 358

Query: 323 SIGPGCANVAIEGVTCGPSHGISSIGSLGVHNSQACVSNITVKNAVIRDSDNGVRIKTWQ 382
           SI  GC+NV +  V CGP HGI SIGSLG  +++ACVSNITV++ V+ ++  GVRIKTWQ
Sbjct: 359 SIQTGCSNVYVHNVNCGPGHGI-SIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQ 418

Query: 383 GGSGSVSNILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYRNIKGTYDVRN 442
           GG GSV  ILF NIQ+  V+  I++DQ+YC    C N TSAV V  V Y  I+GTY V+ 
Sbjct: 419 GGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVK- 478

Query: 443 TPIHFACSDTVACTNITMSEVELLPHEGEL-VEDPFCWNAFGIQETLTIPPIDCLQEGEP 468
            P+HFACSD+  C ++ +S +EL P + +  + DPFCW  FG   + T+PPIDCLQ G+P
Sbjct: 479 -PVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVPPIDCLQIGKP 527

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906648.11.1e-26994.15polygalacturonase At1g48100 [Benincasa hispida][more]
XP_008458305.12.5e-26492.50PREDICTED: polygalacturonase At1g48100 [Cucumis melo] >TYK02978.1 polygalacturon... [more]
XP_004138524.13.2e-26492.50polygalacturonase At1g48100 [Cucumis sativus] >KGN45612.2 hypothetical protein C... [more]
KAG7024752.14.7e-25588.41Polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022930280.12.3e-25488.61polygalacturonase At1g48100-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q949Z11.7e-19670.59Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ53.3e-8643.19Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432122.6e-8344.13Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E78.4e-8245.87Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P353365.4e-8143.49Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BVF71.2e-26492.50Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G006... [more]
A0A1S3C7J51.2e-26492.50polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103497761 PE=3 SV=1[more]
A0A6J1EWI81.1e-25488.61polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 P... [more]
A0A6J1CB483.3e-25488.19polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1KH631.4e-25287.78polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=... [more]
Match NameE-valueIdentityDescription
AT1G48100.11.2e-19770.59Pectin lyase-like superfamily protein [more]
AT1G02460.11.6e-15255.41Pectin lyase-like superfamily protein [more]
AT1G56710.12.1e-14459.07Pectin lyase-like superfamily protein [more]
AT4G01890.13.4e-13956.52Pectin lyase-like superfamily protein [more]
AT1G60590.11.8e-10041.88Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 244..270
e-value: 520.0
score: 9.2
coord: 325..346
e-value: 3.8
score: 16.5
coord: 354..375
e-value: 190.0
score: 10.9
coord: 271..292
e-value: 220.0
score: 10.6
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 74..476
e-value: 9.8E-116
score: 389.1
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 114..431
e-value: 1.0E-83
score: 281.1
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 301..315
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..73
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 7..469
NoneNo IPR availablePANTHERPTHR31375:SF142POLYGALACTURONASE PLANT-LIKE PROTEINcoord: 7..469
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 81..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc06G04520.2Clc06G04520.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0016829 lyase activity
molecular_function GO:0004650 polygalacturonase activity