Clc06G03740 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc06G03740
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	E3 ubiquitin-protein ligase RHA1B
Location	ClcChr06: 3987413 .. 3988922 (+)
RNA-Seq Expression	Clc06G03740
Synteny	Clc06G03740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCACATCCCATTCTATTGGTCATTTCCATCACCGTCATCGCACTAGCATGTGAGTTTATCACCTCCCATTCTCCTTCCTCCCCGTCTCTATATATTAAATCAAATCCCCCTCTTCCCAAACAGAGAGACGCCATTAACCTTACTTCAACCAGTAAATCAAAGAAAATTTCCCACCCGACAGTGACCGGCTTTATGGGTTTTCCGGTGGGTTGCACGGAGGTTTTCTTCCCCACCATATTCCTCCACCTGTTCACTGTCCTTGACGTTCTGAAATTCCTCATCCAGTCCCTCTTCCGCCTCCTGGGGCTGCCGGAATTTCTTCACTCCGATGCTTTCCCACCGGAGCTCCAATTTCCACTCAACCCTCCCCCGTCGGCCCTTCTCCTCCGCGAACTCCTCCCGGTGGTGAAATTCTCGGATCTGCCGGACCCGCCGGAGAGGTGCGCCGTTTGCCTGTACGAGTTCGAAGGGGCGGAGGAGATCCGGTGGCTAACGAACTGCAAGCACATATTTCACCGGAGGTGTGTGGACCGTTGGATGGATCACGATCATGACACGTGTCCACTTTGTAGAACGCCGTTTGTGCCTGATGGGATGATGGACGAATTCAATCAACGGCTGTGGGCTGCCTCCGGTATCGCTGAGACGTTTGAGACGGACTTCAACTCCGTTTTAGGATAGCAAAATGCCAAACCAATTTCACTACACAAGTTTTTTCTTTTTTCCCTTTCCAATTATTCTTTTTCTTTTTTTCTTTTTTTAAGGAAGGTTTTTCAAACTGTATATATTTATAAATATATAATGTTTATATCGTATATATAATATATATGAGAATTTGGGCACTTTTGGCCCAACATGGAAGCTTTTTTCTTTTGAATATGATTGTTTCTTTGCCCCTTCTCTTTTCTCAATTTGTTTAAATGGGTTTTAGAGATATTTGTTGGAGGTAATAAAAGCTTCTATTTCATTTCTTTACTTCTTCAAATTGGAACTGAAATTTGAGGGGATTGATGGGTGTTTTGTGAAGTGAGTGAAATACACTTTGGAGTTTGTATCATATAAAATTATAAAGATTATAATCTCCCTTCTTTAGAATGGAAGTTAATATCAATTACGGTTGAACTCGCTTTGGTGTATCAATTTAAATCTTAAAATTTAAAAAATGAATAATTTAAAATCATTTGTTAATGATTTATAGTTATTTTCACTTGCTATCGTTTAAAATTACTACAATAATTTCATCTTTAGATTTCAATTGGAAATTTGTAAACAATGACGTATGAGCTTTATAATGACAGTTTTAAAATGAAGTGGTAAGTTTGACAGAACATTTATAATTTTACATAAATTTACATACTTTGGAGAGGTATTAACTGTCATCAAAAGTTAGTTGTTCTCCCTCAAAATTCTATCGAAATAAGAAGAAAATTTATGTCATGATTTTTTTTTTCTCTAAATAACTAACAAATAGATAAAGATAAAAAGATGAAACATTGCAACTATCTATAT

mRNA sequence

CTCACATCCCATTCTATTGGTCATTTCCATCACCGTCATCGCACTAGCATGTGAGTTTATCACCTCCCATTCTCCTTCCTCCCCGTCTCTATATATTAAATCAAATCCCCCTCTTCCCAAACAGAGAGACGCCATTAACCTTACTTCAACCAGTAAATCAAAGAAAATTTCCCACCCGACAGTGACCGGCTTTATGGGTTTTCCGGTGGGTTGCACGGAGGTTTTCTTCCCCACCATATTCCTCCACCTGTTCACTGTCCTTGACGTTCTGAAATTCCTCATCCAGTCCCTCTTCCGCCTCCTGGGGCTGCCGGAATTTCTTCACTCCGATGCTTTCCCACCGGAGCTCCAATTTCCACTCAACCCTCCCCCGTCGGCCCTTCTCCTCCGCGAACTCCTCCCGGTGGTGAAATTCTCGGATCTGCCGGACCCGCCGGAGAGGTGCGCCGTTTGCCTGTACGAGTTCGAAGGGGCGGAGGAGATCCGGTGGCTAACGAACTGCAAGCACATATTTCACCGGAGGTGTGTGGACCGTTGGATGGATCACGATCATGACACGTGTCCACTTTGTAGAACGCCGTTTGTGCCTGATGGGATGATGGACGAATTCAATCAACGGCTGTGGGCTGCCTCCGGTATCGCTGAGACGTTTGAGACGGACTTCAACTCCGTTTTAGGATAGCAAAATGCCAAACCAATTTCACTACACAAGTTTTTTCTTTTTTCCCTTTCCAATTATTCTTTTTCTTTTTTTCTTTTTTTAAGGAAGGTTTTTCAAACTGTATATATTTATAAATATATAATGTTTATATCGTATATATAATATATATGAGAATTTGGGCACTTTTGGCCCAACATGGAAGCTTTTTTCTTTTGAATATGATTGTTTCTTTGCCCCTTCTCTTTTCTCAATTTGTTTAAATGGGTTTTAGAGATATTTGTTGGAGGTAATAAAAGCTTCTATTTCATTTCTTTACTTCTTCAAATTGGAACTGAAATTTGAGGGGATTGATGGGTGTTTTGTGAAGTGAGTGAAATACACTTTGGAGTTTGTATCATATAAAATTATAAAGATTATAATCTCCCTTCTTTAGAATGGAAGTTAATATCAATTACGGTTGAACTCGCTTTGGTGTATCAATTTAAATCTTAAAATTTAAAAAATGAATAATTTAAAATCATTTGTTAATGATTTATAGTTATTTTCACTTGCTATCGTTTAAAATTACTACAATAATTTCATCTTTAGATTTCAATTGGAAATTTGTAAACAATGACGTATGAGCTTTATAATGACAGTTTTAAAATGAAGTGGTAAGTTTGACAGAACATTTATAATTTTACATAAATTTACATACTTTGGAGAGGTATTAACTGTCATCAAAAGTTAGTTGTTCTCCCTCAAAATTCTATCGAAATAAGAAGAAAATTTATGTCATGATTTTTTTTTTCTCTAAATAACTAACAAATAGATAAAGATAAAAAGATGAAACATTGCAACTATCTATAT

Coding sequence (CDS)

ATGGGTTTTCCGGTGGGTTGCACGGAGGTTTTCTTCCCCACCATATTCCTCCACCTGTTCACTGTCCTTGACGTTCTGAAATTCCTCATCCAGTCCCTCTTCCGCCTCCTGGGGCTGCCGGAATTTCTTCACTCCGATGCTTTCCCACCGGAGCTCCAATTTCCACTCAACCCTCCCCCGTCGGCCCTTCTCCTCCGCGAACTCCTCCCGGTGGTGAAATTCTCGGATCTGCCGGACCCGCCGGAGAGGTGCGCCGTTTGCCTGTACGAGTTCGAAGGGGCGGAGGAGATCCGGTGGCTAACGAACTGCAAGCACATATTTCACCGGAGGTGTGTGGACCGTTGGATGGATCACGATCATGACACGTGTCCACTTTGTAGAACGCCGTTTGTGCCTGATGGGATGATGGACGAATTCAATCAACGGCTGTGGGCTGCCTCCGGTATCGCTGAGACGTTTGAGACGGACTTCAACTCCGTTTTAGGATAG

Protein sequence

MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPPSALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG

Homology

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BLAST of Clc06G03740 vs. NCBI nr
Match: XP_038875326.1 (probable E3 ubiquitin-protein ligase RHA1A [Benincasa hispida])

HSP 1 Score: 341.7 bits (875), Expect = 3.6e-90
Identity = 159/162 (98.15%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHL +VLDVLK LIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSVLDVLKLLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH 120
           SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 162

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BLAST of Clc06G03740 vs. NCBI nr
Match: XP_008458180.1 (PREDICTED: E3 ubiquitin-protein ligase RHA1B [Cucumis melo] >TYK03034.1 E3 ubiquitin-protein ligase RHA1B [Cucumis melo var. makuwa])

HSP 1 Score: 327.0 bits (837), Expect = 9.1e-86
Identity = 151/162 (93.21%), Postives = 157/162 (96.91%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHL ++LD+LKFLI SLFRLLGLPEFL SD  PPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH 120
           SALLLRELLPVVKFSDLP+PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRC+DRWMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           DTCPLCRTPFVPDGMMDEFNQRLWAASG+AETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

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BLAST of Clc06G03740 vs. NCBI nr
Match: XP_004138493.1 (E3 ubiquitin-protein ligase RHA1B [Cucumis sativus])

HSP 1 Score: 324.3 bits (830), Expect = 5.9e-85
Identity = 152/162 (93.83%), Postives = 155/162 (95.68%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHL ++LDVLKFLI SLFRLLGLPEFL SD  PPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDVLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH 120
           SALLLRELLPVVKFSDLP+PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVD WMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDSWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           DTCPLCRTPFVPD MMDEFNQRLWAASGIAETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDEMMDEFNQRLWAASGIAETFETDFNSVLG 162

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BLAST of Clc06G03740 vs. NCBI nr
Match: XP_023000136.1 (probable E3 ubiquitin-protein ligase RHA1A [Cucurbita maxima])

HSP 1 Score: 277.3 bits (708), Expect = 8.3e-71
Identity = 136/164 (82.93%), Postives = 145/164 (88.41%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSD-AFPPELQFPLNPP 60
           MGFPVGCTEVFFPTIFLHL ++LD+LK LI  LFRLLGLPEFLHSD A  PE+ FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSPEVGFPLNPP 60

Query: 61  PSALLLRELLPVVKFSDLPD-PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDH 120
            SA+LLRE LPVVKFSD+P    E CAVCLYEFEGAEEIRWLTNCKHIFHR CVDRWMDH
Sbjct: 61  ASAVLLREFLPVVKFSDVPQAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMDH 120

Query: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           D  TCPLCRTPFVPDGMMDEFNQRLWAASGIAE F+++FNSVLG
Sbjct: 121 DQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSVLG 163

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BLAST of Clc06G03740 vs. NCBI nr
Match: KAG6593657.1 (Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025989.1 putative E3 ubiquitin-protein ligase RHA1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 269.2 bits (687), Expect = 2.2e-68
Identity = 133/167 (79.64%), Postives = 143/167 (85.63%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSD-AFPPELQFPLNPP 60
           MGFPVGCTEVFFPTIFLHL ++LD+LK LI  LFRLLGLPEFLHSD A   E+  PLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSREVGIPLNPP 60

Query: 61  PSALLLRELLPVVKFSDLPD----PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRW 120
            SA++LRE LPVVKFSD+P       E CAVCLYEFEGAEEIRWLTNCKHIFHR CVDRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           MDHD  TCPLCRTPFVPDGMMDEFNQRLWAASGIAE F+++FNSVLG
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSVLG 166

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BLAST of Clc06G03740 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 8.3e-50
Identity = 94/159 (59.12%), Postives = 115/159 (72.33%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSD-AFPPELQFPLNPP 60
           MGFPVG TEVF P +F+   ++L  ++ ++ S+FR LGL +FL  D  +P    +P   P
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  P-----SALLLRELLPVVKFSDL----PDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRR 120
                 SALL+RE+LPV+KF +L     D PE CAVCLYEFEG +EIRWL NC+HIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CVDRWMDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGI 150
           C+DRWMDHD  TCPLCRTPFVPD M +EFNQRLWAASG+
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASGV 159

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BLAST of Clc06G03740 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.4e-20
Identity = 58/151 (38.41%), Postives = 90/151 (59.60%), Query Frame = 0


Query: 1   MGFPVG-CTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFL-HSDAFPPEL-QFPLN 60
           MG P    TE+  P+  L +  V+   + ++ +L   +GLP FL H++   P+L +  L+
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  PPPSALLLRELLPVVKFSDLP-DPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRW- 120
              SA L  EL+PVV+FSDLP DP + C VCL +FE  +++R L  C H+FH  C+DRW 
Sbjct: 61  --TSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWI 120

Query: 121 MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAA 147
           +D++   CP+CR  F+P    +++ Q  W +
Sbjct: 121 VDYNKMKCPVCRHRFLP---KEKYTQSDWGS 146

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BLAST of Clc06G03740 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.4e-19
Identity = 56/149 (37.58%), Postives = 78/149 (52.35%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MG P    E+  P   L    V+   + ++ +L   +GLP FL  +         L    
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALST 60

Query: 61  SALLLRELLPVVKFSD-LPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRW-MDH 120
           SA L  EL+PVV+FSD L DP + C VCL +F   ++IR L  C H+FH RC+DRW +D 
Sbjct: 61  SATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDC 120

Query: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAAS 148
           +  TCP+CR  F+P+     F+   W  S
Sbjct: 121 NKITCPICRNRFLPEEKSTPFD---WGTS 146

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BLAST of Clc06G03740 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 4.5e-11
Identity = 34/72 (47.22%), Postives = 41/72 (56.94%), Query Frame = 0


Query: 67  ELLPVVKFSDLPDPPE--RCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDHDTCP 126
           E LPV +F  L    +   CAVCL  FE  E +R L  CKH FH  CVD W+D  H TCP
Sbjct: 127 ESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVDTWLD-AHSTCP 186

Query: 127 LCRTPFVPDGMM 137
           LCR    P+ ++
Sbjct: 187 LCRYRVDPEDIL 197

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BLAST of Clc06G03740 vs. ExPASy Swiss-Prot
Match: Q6NML0 (E3 ubiquitin-protein ligase ATL76 OS=Arabidopsis thaliana OX=3702 GN=ATL76 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-10
Identity = 43/122 (35.25%), Postives = 61/122 (50.00%), Query Frame = 0


Query: 22  VLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPPSAL-------------LLREL 81
           VL +L  LI  +   LGL  ++   AF    +F ++ P S+L                 +
Sbjct: 58  VLMLLSVLICGIICCLGL-HYIIRCAFRRSSRFMISEPISSLSTPRSSSNKGIKKKALRM 117

Query: 82  LPVVKFS---DLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDHDTCPL 128
            PVV +S   +LP   E C +CL +F   E++R L  C H FH RC+D+W+ H H TCP 
Sbjct: 118 FPVVSYSREMNLPGIGEECVICLSDFVSGEQLRLLPKCNHGFHVRCIDKWLQH-HLTCPK 177

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BLAST of Clc06G03740 vs. ExPASy TrEMBL
Match: A0A5D3BV35 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001290 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 4.4e-86
Identity = 151/162 (93.21%), Postives = 157/162 (96.91%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHL ++LD+LKFLI SLFRLLGLPEFL SD  PPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH 120
           SALLLRELLPVVKFSDLP+PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRC+DRWMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           DTCPLCRTPFVPDGMMDEFNQRLWAASG+AETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

[Close]

BLAST of Clc06G03740 vs. ExPASy TrEMBL
Match: A0A1S3C782 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 4.4e-86
Identity = 151/162 (93.21%), Postives = 157/162 (96.91%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHL ++LD+LKFLI SLFRLLGLPEFL SD  PPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH 120
           SALLLRELLPVVKFSDLP+PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRC+DRWMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           DTCPLCRTPFVPDGMMDEFNQRLWAASG+AETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

[Close]

BLAST of Clc06G03740 vs. ExPASy TrEMBL
Match: A0A0A0K874 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.9e-85
Identity = 152/162 (93.83%), Postives = 155/162 (95.68%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHL ++LDVLKFLI SLFRLLGLPEFL SD  PPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDVLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH 120
           SALLLRELLPVVKFSDLP+PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVD WMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDSWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           DTCPLCRTPFVPD MMDEFNQRLWAASGIAETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDEMMDEFNQRLWAASGIAETFETDFNSVLG 162

[Close]

BLAST of Clc06G03740 vs. ExPASy TrEMBL
Match: A0A6J1KF16 (probable E3 ubiquitin-protein ligase RHA1A OS=Cucurbita maxima OX=3661 GN=LOC111494424 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.0e-71
Identity = 136/164 (82.93%), Postives = 145/164 (88.41%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSD-AFPPELQFPLNPP 60
           MGFPVGCTEVFFPTIFLHL ++LD+LK LI  LFRLLGLPEFLHSD A  PE+ FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSPEVGFPLNPP 60

Query: 61  PSALLLRELLPVVKFSDLPD-PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDH 120
            SA+LLRE LPVVKFSD+P    E CAVCLYEFEGAEEIRWLTNCKHIFHR CVDRWMDH
Sbjct: 61  ASAVLLREFLPVVKFSDVPQAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMDH 120

Query: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           D  TCPLCRTPFVPDGMMDEFNQRLWAASGIAE F+++FNSVLG
Sbjct: 121 DQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSVLG 163

[Close]

BLAST of Clc06G03740 vs. ExPASy TrEMBL
Match: A0A6J1HIP9 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111464396 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.6e-67
Identity = 132/167 (79.04%), Postives = 142/167 (85.03%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSD-AFPPELQFPLNPP 60
           MGFPVGCTEVFFPTIFLHL ++LD+LK LI  LFRLLGLPEFL+SD A   E   PLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLYSDAAISREGGIPLNPP 60

Query: 61  PSALLLRELLPVVKFSDLPD----PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRW 120
            SA++LRE LPVVKFSD+P       E CAVCLYEFEGAEEIRWLTNCKHIFHR CVDRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 163
           MDHD  TCPLCRTPFVPDGMMDEFNQRLWAASGIAE F+++FNSVLG
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSVLG 166

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BLAST of Clc06G03740 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 198.0 bits (502), Expect = 5.9e-51
Identity = 94/159 (59.12%), Postives = 115/159 (72.33%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSD-AFPPELQFPLNPP 60
           MGFPVG TEVF P +F+   ++L  ++ ++ S+FR LGL +FL  D  +P    +P   P
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  P-----SALLLRELLPVVKFSDL----PDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRR 120
                 SALL+RE+LPV+KF +L     D PE CAVCLYEFEG +EIRWL NC+HIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CVDRWMDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGI 150
           C+DRWMDHD  TCPLCRTPFVPD M +EFNQRLWAASG+
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASGV 159

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BLAST of Clc06G03740 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 5.2e-39
Identity = 82/168 (48.81%), Postives = 114/168 (67.86%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MGFPVG +E+  P IF +L + L +++ LI ++F+++GLP+FL  +  P    +P +PPP
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPE--PVSTSWP-DPPP 60

Query: 61  -------SALLLRELLPVVKFSDLPDP-PERCAVCLYEFEGAEEIRWLTNCKHIFHRRCV 120
                  +A+L  E+LPVV+FSD+  P  E CAVCLY+FE  +EIR LTNC+HIFHR C+
Sbjct: 61  TLTKPDSAAILAGEMLPVVRFSDINRPESECCAVCLYDFENDDEIRRLTNCRHIFHRGCL 120

Query: 121 DRW-MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNS 160
           DRW M ++  TCPLCRT F+PD +  EFNQRLW+ S    +   D +S
Sbjct: 121 DRWMMGYNQMTCPLCRTQFIPDHLQLEFNQRLWSQSSAVSSQLLDESS 165

[Close]

BLAST of Clc06G03740 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 150.2 bits (378), Expect = 1.4e-36
Identity = 80/169 (47.34%), Postives = 108/169 (63.91%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAF--------PPEL 60
           MG+PVG TE+  P IFLHL ++L +++ LI + FR+LGLP+FL SD          PP +
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYM 60

Query: 61  QFPLNP--------PPSALLLRELLPVVKFSDLPDP-----PERCAVCLYEFEGAEEIRW 120
               +         P +A L  E+LPV++FS+L  P      + CAVCL+EFE  +EIR 
Sbjct: 61  STAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LTNCKHIFHRRCVDRW-MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAAS 148
           LTNC+HIFHR C+DRW M ++  TCPLCRTPF+ D +   FNQR+W+ S
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVAFNQRVWSES 169

[Close]

BLAST of Clc06G03740 vs. TAIR 10
Match: AT4G11370.1 (RING-H2 finger A1A )

HSP 1 Score: 100.1 bits (248), Expect = 1.7e-21
Identity = 58/151 (38.41%), Postives = 90/151 (59.60%), Query Frame = 0


Query: 1   MGFPVG-CTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFL-HSDAFPPEL-QFPLN 60
           MG P    TE+  P+  L +  V+   + ++ +L   +GLP FL H++   P+L +  L+
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  PPPSALLLRELLPVVKFSDLP-DPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRW- 120
              SA L  EL+PVV+FSDLP DP + C VCL +FE  +++R L  C H+FH  C+DRW 
Sbjct: 61  --TSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWI 120

Query: 121 MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAA 147
           +D++   CP+CR  F+P    +++ Q  W +
Sbjct: 121 VDYNKMKCPVCRHRFLP---KEKYTQSDWGS 146

[Close]

BLAST of Clc06G03740 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 96.3 bits (238), Expect = 2.4e-20
Identity = 56/149 (37.58%), Postives = 78/149 (52.35%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHLFTVLDVLKFLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60
           MG P    E+  P   L    V+   + ++ +L   +GLP FL  +         L    
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALST 60

Query: 61  SALLLRELLPVVKFSD-LPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRW-MDH 120
           SA L  EL+PVV+FSD L DP + C VCL +F   ++IR L  C H+FH RC+DRW +D 
Sbjct: 61  SATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDC 120

Query: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAAS 148
           +  TCP+CR  F+P+     F+   W  S
Sbjct: 121 NKITCPICRNRFLPEEKSTPFD---WGTS 146

The following BLAST results are available for this feature:

BLAST of Clc06G03740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875326.1	3.6e-90	98.15	probable E3 ubiquitin-protein ligase RHA1A [Benincasa hispida]	[more]
XP_008458180.1	9.1e-86	93.21	PREDICTED: E3 ubiquitin-protein ligase RHA1B [Cucumis melo] >TYK03034.1 E3 ubiqu...	[more]
XP_004138493.1	5.9e-85	93.83	E3 ubiquitin-protein ligase RHA1B [Cucumis sativus]	[more]
XP_023000136.1	8.3e-71	82.93	probable E3 ubiquitin-protein ligase RHA1A [Cucurbita maxima]	[more]
KAG6593657.1	2.2e-68	79.64	Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of Clc06G03740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	8.3e-50	59.12	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS4	2.4e-20	38.41	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SUS5	3.4e-19	37.58	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q5EAE9	4.5e-11	47.22	RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2	[more]
Q6NML0	2.2e-10	35.25	E3 ubiquitin-protein ligase ATL76 OS=Arabidopsis thaliana OX=3702 GN=ATL76 PE=1 ...	[more]

BLAST of Clc06G03740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BV35	4.4e-86	93.21	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3C782	4.4e-86	93.21	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 S...	[more]
A0A0A0K874	2.9e-85	93.83	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 P...	[more]
A0A6J1KF16	4.0e-71	82.93	probable E3 ubiquitin-protein ligase RHA1A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1HIP9	1.6e-67	79.04	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]

BLAST of Clc06G03740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61460.1	5.9e-51	59.12	brassinosteroid-responsive RING-H2	[more]
AT1G63840.1	5.2e-39	48.81	RING/U-box superfamily protein	[more]
AT5G41400.1	1.4e-36	47.34	RING/U-box superfamily protein	[more]
AT4G11370.1	1.7e-21	38.41	RING-H2 finger A1A	[more]
AT4G11360.1	2.4e-20	37.58	RING-H2 finger A1B	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 84..126 e-value: 6.6E-6 score: 35.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 83..127 e-value: 1.7E-11 score: 44.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 84..127 score: 12.356501
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 67..133 e-value: 2.7E-17 score: 63.7
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 1..153
None	No IPR available	PANTHER	PTHR45969:SF10	BRH1 RING FINGER PROTEIN	coord: 1..153
None	No IPR available	CDD	cd16448	RING-H2	coord: 84..127 e-value: 6.25818E-14 score: 60.5435
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 69..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc06G03740.1	Clc06G03740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity