Clc06G00870 (gene) Watermelon (cordophanus) v2

Overview
NameClc06G00870
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationClcChr06: 791069 .. 791999 (-)
RNA-Seq ExpressionClc06G00870
SyntenyClc06G00870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAATCACTTCAAAATTTACCCATACAAAGGAAGAATAATTAATATCCATCATGAAACCCAAATTCTCACTATCTCCTTTCATGATCACTCCTCTCCCTTCTCTTCTCCTTCTCTCCATCATCATCTTCAACATCTCTACCACAATTGTTGTTGCTGATCTTGTCCAAAAAACTTGCAAAAAATGCGAAAACAACGACCCGAACATCAACTACAACTTCTGCATTTCATCTTTCCATGCTCATCCAGGAAGCGATACGGCCGATCTTCGAAAACTCGGTGCCATTTCATTGAGTTTGATTCAAAAAAATGTTAGCAACTCATTTGAGTATGTGGAAAAGCTATTACAAAACAAAGAAATAGACTCATATAAAAAGCTTTGCTTAAATGATTGTTTAGAAGTCTATTCAAATGCCATTGATAGTATGGAAGAAGGAAAGAAAGCATACAAGGAAAAACATTACAATGATGCTAACATCAAAGTGAGCTCAGTGATGGATGCTGCTAGGGTTTGTGAGGATGGGTTTAGGGAAAAAGAAGGTGTCTCCTCGCCATTGACAAAGTGGAATAAGGACTTGTTTCAATTGGCTGCCATTGCTCTTTCAATCATCAATATGTATCCATAAAGACGACATATTATTCAAGATGTGATTGCCACAACGTGTCTGACAATTTTACCTGTTGATTGGATGCATGAGTGAAGAAGAAATGTTGTAACATTATTTTGATTTTGTATCCAATATATTGGGTGTTATAGGAAAATATAAATTGTGGCGACCTTCCTGATAATGTTTAAATTAAGGTTATCTTTTTCACAAACAAACTATAATACGTACAATAATTTGTCTTTTTGTTTTTATCAATAAGCTTGTTAAACAAAAGTTCTATATTATACTTTGTAAAAATCTAAATAGTTAATATGATA

mRNA sequence

AAAAAAAAAAAATCACTTCAAAATTTACCCATACAAAGGAAGAATAATTAATATCCATCATGAAACCCAAATTCTCACTATCTCCTTTCATGATCACTCCTCTCCCTTCTCTTCTCCTTCTCTCCATCATCATCTTCAACATCTCTACCACAATTGTTGTTGCTGATCTTGTCCAAAAAACTTGCAAAAAATGCGAAAACAACGACCCGAACATCAACTACAACTTCTGCATTTCATCTTTCCATGCTCATCCAGGAAGCGATACGGCCGATCTTCGAAAACTCGGTGCCATTTCATTGAGTTTGATTCAAAAAAATGTTAGCAACTCATTTGAGTATGTGGAAAAGCTATTACAAAACAAAGAAATAGACTCATATAAAAAGCTTTGCTTAAATGATTGTTTAGAAGTCTATTCAAATGCCATTGATAGTATGGAAGAAGGAAAGAAAGCATACAAGGAAAAACATTACAATGATGCTAACATCAAAGTGAGCTCAGTGATGGATGCTGCTAGGGTTTGTGAGGATGGGTTTAGGGAAAAAGAAGGTGTCTCCTCGCCATTGACAAAGTGGAATAAGGACTTGTTTCAATTGGCTGCCATTGCTCTTTCAATCATCAATATGTATCCATAAAGACGACATATTATTCAAGATGTGATTGCCACAACGTGTCTGACAATTTTACCTGTTGATTGGATGCATGAGTGAAGAAGAAATGTTGTAACATTATTTTGATTTTGTATCCAATATATTGGGTGTTATAGGAAAATATAAATTGTGGCGACCTTCCTGATAATGTTTAAATTAAGGTTATCTTTTTCACAAACAAACTATAATACGTACAATAATTTGTCTTTTTGTTTTTATCAATAAGCTTGTTAAACAAAAGTTCTATATTATACTTTGTAAAAATCTAAATAGTTAATATGATA

Coding sequence (CDS)

ATGAAACCCAAATTCTCACTATCTCCTTTCATGATCACTCCTCTCCCTTCTCTTCTCCTTCTCTCCATCATCATCTTCAACATCTCTACCACAATTGTTGTTGCTGATCTTGTCCAAAAAACTTGCAAAAAATGCGAAAACAACGACCCGAACATCAACTACAACTTCTGCATTTCATCTTTCCATGCTCATCCAGGAAGCGATACGGCCGATCTTCGAAAACTCGGTGCCATTTCATTGAGTTTGATTCAAAAAAATGTTAGCAACTCATTTGAGTATGTGGAAAAGCTATTACAAAACAAAGAAATAGACTCATATAAAAAGCTTTGCTTAAATGATTGTTTAGAAGTCTATTCAAATGCCATTGATAGTATGGAAGAAGGAAAGAAAGCATACAAGGAAAAACATTACAATGATGCTAACATCAAAGTGAGCTCAGTGATGGATGCTGCTAGGGTTTGTGAGGATGGGTTTAGGGAAAAAGAAGGTGTCTCCTCGCCATTGACAAAGTGGAATAAGGACTTGTTTCAATTGGCTGCCATTGCTCTTTCAATCATCAATATGTATCCATAA

Protein sequence

MKPKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIINMYP
Homology
BLAST of Clc06G00870 vs. NCBI nr
Match: XP_008460198.2 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 324.3 bits (830), Expect = 6.9e-85
Identity = 164/190 (86.32%), Postives = 179/190 (94.21%), Query Frame = 0

Query: 1   MKPKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISS 60
           MKPKFSLSPFM TPLP LLL  I+IFNISTTIVVADLVQKTCKKCE +DPN+NYNFCISS
Sbjct: 1   MKPKFSLSPFMTTPLPCLLLF-IMIFNISTTIVVADLVQKTCKKCETDDPNVNYNFCISS 60

Query: 61  FHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSN 120
           F AH GSD+ DLRKLGAISLSLIQKN+S+S EYVEKLLQNKEIDSY+++ LNDCL+VYS+
Sbjct: 61  FRAHSGSDSTDLRKLGAISLSLIQKNLSSSLEYVEKLLQNKEIDSYRRVRLNDCLDVYSD 120

Query: 121 AIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180
           AI ++EEGKKAYKEKHY+DANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA
Sbjct: 121 AIVNVEEGKKAYKEKHYDDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180

Query: 181 IALSIINMYP 191
           IALSI+NMYP
Sbjct: 181 IALSIVNMYP 189

BLAST of Clc06G00870 vs. NCBI nr
Match: XP_038875624.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 322.0 bits (824), Expect = 3.4e-84
Identity = 168/190 (88.42%), Postives = 176/190 (92.63%), Query Frame = 0

Query: 1   MKPKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISS 60
           MKPKFSLS FMITPL SLLLL  IIFNISTTIVV DLVQKTCKKCENNDPNINY FCISS
Sbjct: 1   MKPKFSLSSFMITPLSSLLLL-YIIFNISTTIVVGDLVQKTCKKCENNDPNINYIFCISS 60

Query: 61  FHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSN 120
           F AH GSD+ADLRKLGAISLSLIQKN+SNSF YVEKLLQNKEIDSYK++ LNDCLEVYS+
Sbjct: 61  FRAHSGSDSADLRKLGAISLSLIQKNLSNSFGYVEKLLQNKEIDSYKRVRLNDCLEVYSD 120

Query: 121 AIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180
           AI S+EEGKKAY  KHYNDANIKVSSVMDAARVCEDGF+EKEGVSSPLT+WNKD FQLAA
Sbjct: 121 AIVSVEEGKKAYIAKHYNDANIKVSSVMDAARVCEDGFKEKEGVSSPLTRWNKDFFQLAA 180

Query: 181 IALSIINMYP 191
           IALSIINMYP
Sbjct: 181 IALSIINMYP 189

BLAST of Clc06G00870 vs. NCBI nr
Match: XP_004145080.2 (putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 309.3 bits (791), Expect = 2.3e-80
Identity = 159/190 (83.68%), Postives = 175/190 (92.11%), Query Frame = 0

Query: 1   MKPKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISS 60
           MKPKF  SPFM TPLP LLL  IIIFNISTT VVADLVQK CKKCE +DPNINYNFC SS
Sbjct: 1   MKPKF--SPFMTTPLPCLLLF-IIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSS 60

Query: 61  FHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSN 120
           F AH GSD+ DLRKLGAISLSLIQ+N+S+SFEYVEKLLQNKEIDSYK++ LNDCL+VYS+
Sbjct: 61  FRAHSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSD 120

Query: 121 AIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180
           AI ++EEGKKAYKEKHY+DANIKVS+VMD+ARVCEDGFREKEGVSSPLTKWNKD+FQLAA
Sbjct: 121 AIVTVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAA 180

Query: 181 IALSIINMYP 191
           IALSIINM+P
Sbjct: 181 IALSIINMHP 187

BLAST of Clc06G00870 vs. NCBI nr
Match: KAE8646717.1 (hypothetical protein Csa_005489 [Cucumis sativus])

HSP 1 Score: 305.4 bits (781), Expect = 3.3e-79
Identity = 157/188 (83.51%), Postives = 173/188 (92.02%), Query Frame = 0

Query: 3   PKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISSFH 62
           PKF  SPFM TPLP LLL  IIIFNISTT VVADLVQK CKKCE +DPNINYNFC SSF 
Sbjct: 167 PKF--SPFMTTPLPCLLLF-IIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSSFR 226

Query: 63  AHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAI 122
           AH GSD+ DLRKLGAISLSLIQ+N+S+SFEYVEKLLQNKEIDSYK++ LNDCL+VYS+AI
Sbjct: 227 AHSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSDAI 286

Query: 123 DSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIA 182
            ++EEGKKAYKEKHY+DANIKVS+VMD+ARVCEDGFREKEGVSSPLTKWNKD+FQLAAIA
Sbjct: 287 VTVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAAIA 346

Query: 183 LSIINMYP 191
           LSIINM+P
Sbjct: 347 LSIINMHP 351

BLAST of Clc06G00870 vs. NCBI nr
Match: KAA0039958.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK24545.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 304.7 bits (779), Expect = 5.7e-79
Identity = 154/180 (85.56%), Postives = 169/180 (93.89%), Query Frame = 0

Query: 11  MITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTA 70
           M TPLP LLL  I+IFNISTTIVVADLVQKTCKKCE +DPN+NYNFCISSF AH GSD+ 
Sbjct: 1   MTTPLPCLLLF-IMIFNISTTIVVADLVQKTCKKCETDDPNVNYNFCISSFRAHSGSDST 60

Query: 71  DLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKK 130
           DLRKLGAISLSLIQKN+S+S EYVEKLLQNKEIDSY+++ LNDCL+VYS+AI ++EEGKK
Sbjct: 61  DLRKLGAISLSLIQKNLSSSLEYVEKLLQNKEIDSYRRVRLNDCLDVYSDAIVNVEEGKK 120

Query: 131 AYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIINMYP 190
           AYKEKHY+DANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSI+NMYP
Sbjct: 121 AYKEKHYDDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIVNMYP 179

BLAST of Clc06G00870 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 9.2e-32
Identity = 73/171 (42.69%), Postives = 110/171 (64.33%), Query Frame = 0

Query: 21  LSIIIFN---ISTTIVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTADLRKLGA 80
           L I  FN   +   ++ AD+VQ TCKK     PN+NY+FC+ S  A P S TADL+ LG 
Sbjct: 7   LCIFFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGV 66

Query: 81  ISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKKAYKEKHY 140
           IS +L  ++ S    ++ ++L++K +D   K  LNDC+ +Y++A  S++E    +K K Y
Sbjct: 67  ISANLAIQHGSKIQTFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDY 126

Query: 141 NDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIINM 189
             AN+K+S+ +D +  CEDGF+EK+G+ SP+TK NKD  QL AI+L+I  +
Sbjct: 127 ASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

BLAST of Clc06G00870 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.6e-31
Identity = 68/157 (43.31%), Postives = 105/157 (66.88%), Query Frame = 0

Query: 32  IVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTADLRKLGAISLSLIQKNVSNSF 91
           ++ AD+VQ TCKK     PN+NY+FC+ S  A P S +ADL+ LG IS +L  +  S   
Sbjct: 12  LISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKIQ 71

Query: 92  EYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKKAYKEKHYNDANIKVSSVMDAA 151
            ++ ++L++K +D   K  LNDC+ +Y++A  S++E    +K K Y  AN+K+S+ +D +
Sbjct: 72  TFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDS 131

Query: 152 RVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIINM 189
             CEDGF+EK+G++SP+TK NKD  QL AI+L+I  +
Sbjct: 132 VTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

BLAST of Clc06G00870 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.7e-12
Identity = 52/172 (30.23%), Postives = 85/172 (49.42%), Query Frame = 0

Query: 20  LLSIIIFN--ISTTIVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTA-DLRKLG 79
           L+S++IF+  ++       L+Q +CKK    DP ++Y+FC++S    P S  A  L  L 
Sbjct: 4   LVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLV 63

Query: 80  AISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKKAYKEKH 139
             S       ++N    V + L+++      +  L  CL  Y++A D +       K + 
Sbjct: 64  LASTKTAAAKITNLKGIVAQDLKDQRYQDIVE-DLKLCLGFYNDANDDLTTALANIKSRD 123

Query: 140 YNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIINM 189
           Y  ANI +S+ +D    CED F+E +  +SP+T  N  LF+   I L+  NM
Sbjct: 124 YQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

BLAST of Clc06G00870 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.3e-08
Identity = 47/180 (26.11%), Postives = 83/180 (46.11%), Query Frame = 0

Query: 11  MITPLPSLLLLSIIIFNISTTIVVADL--VQKTCKKCENNDPNINYNFCISSFHAHPGSD 70
           M   L + +L+S ++F + T  + A +  ++  C K +N       +FC S   ++P + 
Sbjct: 1   MAAYLTNRVLMSSLMFFVMTGSLNAQVADIKAICGKAKNQ------SFCTSYMKSNPKTS 60

Query: 71  TADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEG 130
            ADL+ L  I+    Q + S  F  ++ L++     + KK     C++ Y +AI S+ + 
Sbjct: 61  GADLQTLANITFGSAQTSASEGFRKIQSLVKTATNPTMKK-AYTSCVQHYKSAISSLNDA 120

Query: 131 KKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIINM 189
           K++         NIKVS+ M+    CE    + + V     K + D   +  I L I NM
Sbjct: 121 KQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFK-VDPSAVKNSGDFQNICGIVLVISNM 172

BLAST of Clc06G00870 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-07
Identity = 45/161 (27.95%), Postives = 76/161 (47.20%), Query Frame = 0

Query: 35  ADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYV 94
           A LV+ TC      +    Y+ C+S+  A P S TAD+R L AI++S+   N S + +  
Sbjct: 59  AALVRATC------NSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAA 118

Query: 95  EKLLQNKEI-------DSYKKLCLNDCLEVYSNAIDSMEEGKKAYKEKHYNDANIKVSSV 154
             L             D   +  L  C   Y +A D++   K++  ++ Y+ A + VS+ 
Sbjct: 119 AALANGTAPLAAAAAGDGTVQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAG 178

Query: 155 MDAARVCEDGFREKEGVSSP--LTKWNKDLFQLAAIALSII 187
            +  +VC+  FR +   + P  L    + L +L ++AL II
Sbjct: 179 AEYPQVCKALFRRQRPGAYPAELAAREEALNKLCSVALDII 213

BLAST of Clc06G00870 vs. ExPASy TrEMBL
Match: A0A1S3CCF7 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103499083 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.3e-85
Identity = 164/190 (86.32%), Postives = 179/190 (94.21%), Query Frame = 0

Query: 1   MKPKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISS 60
           MKPKFSLSPFM TPLP LLL  I+IFNISTTIVVADLVQKTCKKCE +DPN+NYNFCISS
Sbjct: 1   MKPKFSLSPFMTTPLPCLLLF-IMIFNISTTIVVADLVQKTCKKCETDDPNVNYNFCISS 60

Query: 61  FHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSN 120
           F AH GSD+ DLRKLGAISLSLIQKN+S+S EYVEKLLQNKEIDSY+++ LNDCL+VYS+
Sbjct: 61  FRAHSGSDSTDLRKLGAISLSLIQKNLSSSLEYVEKLLQNKEIDSYRRVRLNDCLDVYSD 120

Query: 121 AIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180
           AI ++EEGKKAYKEKHY+DANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA
Sbjct: 121 AIVNVEEGKKAYKEKHYDDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180

Query: 181 IALSIINMYP 191
           IALSI+NMYP
Sbjct: 181 IALSIVNMYP 189

BLAST of Clc06G00870 vs. ExPASy TrEMBL
Match: A0A0A0K9X7 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080360 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.1e-80
Identity = 159/190 (83.68%), Postives = 175/190 (92.11%), Query Frame = 0

Query: 1   MKPKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISS 60
           MKPKF  SPFM TPLP LLL  IIIFNISTT VVADLVQK CKKCE +DPNINYNFC SS
Sbjct: 1   MKPKF--SPFMTTPLPCLLLF-IIIFNISTTFVVADLVQKACKKCEIDDPNINYNFCTSS 60

Query: 61  FHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSN 120
           F AH GSD+ DLRKLGAISLSLIQ+N+S+SFEYVEKLLQNKEIDSYK++ LNDCL+VYS+
Sbjct: 61  FRAHSGSDSTDLRKLGAISLSLIQRNLSSSFEYVEKLLQNKEIDSYKRVRLNDCLDVYSD 120

Query: 121 AIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180
           AI ++EEGKKAYKEKHY+DANIKVS+VMD+ARVCEDGFREKEGVSSPLTKWNKD+FQLAA
Sbjct: 121 AIVTVEEGKKAYKEKHYDDANIKVSAVMDSARVCEDGFREKEGVSSPLTKWNKDMFQLAA 180

Query: 181 IALSIINMYP 191
           IALSIINM+P
Sbjct: 181 IALSIINMHP 187

BLAST of Clc06G00870 vs. ExPASy TrEMBL
Match: A0A5A7TAH1 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00900 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.7e-79
Identity = 154/180 (85.56%), Postives = 169/180 (93.89%), Query Frame = 0

Query: 11  MITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTA 70
           M TPLP LLL  I+IFNISTTIVVADLVQKTCKKCE +DPN+NYNFCISSF AH GSD+ 
Sbjct: 1   MTTPLPCLLLF-IMIFNISTTIVVADLVQKTCKKCETDDPNVNYNFCISSFRAHSGSDST 60

Query: 71  DLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKK 130
           DLRKLGAISLSLIQKN+S+S EYVEKLLQNKEIDSY+++ LNDCL+VYS+AI ++EEGKK
Sbjct: 61  DLRKLGAISLSLIQKNLSSSLEYVEKLLQNKEIDSYRRVRLNDCLDVYSDAIVNVEEGKK 120

Query: 131 AYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIINMYP 190
           AYKEKHY+DANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSI+NMYP
Sbjct: 121 AYKEKHYDDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIVNMYP 179

BLAST of Clc06G00870 vs. ExPASy TrEMBL
Match: A0A6J1KJ45 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494454 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.1e-67
Identity = 137/188 (72.87%), Postives = 151/188 (80.32%), Query Frame = 0

Query: 1   MKPKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISS 60
           M PK S  PFMI       ++S+IIFNISTTIVVAD VQKTCKKCE N+PN+NY FC+SS
Sbjct: 1   MNPKSSSFPFMI-------IISMIIFNISTTIVVADFVQKTCKKCEKNNPNVNYEFCVSS 60

Query: 61  FHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSN 120
           F +HPGSD+ADLRKLGAISL LI  NVS S EYVE LL  KE+D YK+  LNDCLEVY  
Sbjct: 61  FRSHPGSDSADLRKLGAISLHLIGNNVSRSVEYVEDLLHKKEVDPYKRARLNDCLEVYCE 120

Query: 121 AIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180
           A  S+EEGKKAYKE  Y+D NIKVSSVMDA RVCEDGFREKEGVSSPLT+WN D F+L A
Sbjct: 121 AAVSVEEGKKAYKEGRYDDVNIKVSSVMDAGRVCEDGFREKEGVSSPLTRWNNDTFKLTA 180

Query: 181 IALSIINM 189
           IALSIINM
Sbjct: 181 IALSIINM 181

BLAST of Clc06G00870 vs. ExPASy TrEMBL
Match: A0A6J1HKS5 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464448 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 9.1e-67
Identity = 134/188 (71.28%), Postives = 152/188 (80.85%), Query Frame = 0

Query: 1   MKPKFSLSPFMITPLPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISS 60
           M PK S  PFMI       ++S+II NISTTIVVA+ V+KTCKKCE N+PN++Y FC+SS
Sbjct: 3   MNPKSSSFPFMI-------IISMIILNISTTIVVANFVEKTCKKCEKNNPNVDYEFCVSS 62

Query: 61  FHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSN 120
           F +HPGSD+ADLRKLGAISL LI KNVSNS EYVE LL  KE+D YK+  LNDCLEVY  
Sbjct: 63  FRSHPGSDSADLRKLGAISLHLIGKNVSNSVEYVEDLLHKKEVDPYKRARLNDCLEVYCE 122

Query: 121 AIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAA 180
           A+ S+EEGKKAYKE  Y+D NIKVSSVMDA RVCEDGFREKEG+SSPLT+WN D F L A
Sbjct: 123 AVVSVEEGKKAYKEGRYDDVNIKVSSVMDAGRVCEDGFREKEGLSSPLTRWNNDTFNLTA 182

Query: 181 IALSIINM 189
           IALSIINM
Sbjct: 183 IALSIINM 183

BLAST of Clc06G00870 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 8.3e-20
Identity = 57/187 (30.48%), Postives = 96/187 (51.34%), Query Frame = 0

Query: 15  LPSLLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGS--DTADL 74
           L  +  +  + F +S     +D++ +TCK C       +YNFC+SS +  P +     +L
Sbjct: 3   LSQVFYIIFLFFLVSQVKSTSDMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNL 62

Query: 75  RKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKKAY 134
             L  + +     N + +   +++LL + +  S++  CL DCLE+Y +A D +EE  + +
Sbjct: 63  SSLALVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVF 122

Query: 135 -KEKHYNDANIKVSSVMDAARVCEDGFREKE-------GV---SSPLTKWNKDLFQLAAI 189
              K     N+ VS+ M++A  CE+GFRE++       GV   +SP+   N  LF+   I
Sbjct: 123 ITRKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQI 182

BLAST of Clc06G00870 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.4e-19
Identity = 54/161 (33.54%), Postives = 86/161 (53.42%), Query Frame = 0

Query: 18  LLLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTA-DLRKLG 77
           L   ++    ++   +   L+Q  CKK  + +P I+YNFC+ S   +P S TA  L +L 
Sbjct: 4   LFYFAMFFLLLNAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLV 63

Query: 78  AISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKKAYKEKH 137
            +S        ++    V+K+L+    + Y +  L DCLE+YS+A +S++E     K + 
Sbjct: 64  MLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSRD 123

Query: 138 YNDANIKVSSVMDAARVCEDGFRE-KEGVSSPLTKWNKDLF 177
           Y  AN+ +S+ M A   CE GF+E K+ + SP TK N  LF
Sbjct: 124 YKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

BLAST of Clc06G00870 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.0e-18
Identity = 53/172 (30.81%), Postives = 89/172 (51.74%), Query Frame = 0

Query: 19  LLLSIIIFNISTTIVVAD-LVQKTCKKCENNDPNINYNFCISSFHAHPGSDTA-DLRKLG 78
           LL  +  F +S  +     L++ +CKK     P   YN C++S   +P + TA DL  L 
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLV 63

Query: 79  AISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKKAYKEKH 138
             S        +     V+K+++ K+++    + L DCL++Y++AI S+ E     K ++
Sbjct: 64  MASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRN 123

Query: 139 YNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDLFQLAAIALSIINM 189
           Y      +S+ MD    CE GF+E++   SP+TK N +L+Q+  I L+  NM
Sbjct: 124 YPTVKTVLSAAMDTPSTCETGFKERK-APSPVTKENDNLYQMILIPLAFTNM 174

BLAST of Clc06G00870 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 3.9e-17
Identity = 54/172 (31.40%), Postives = 88/172 (51.16%), Query Frame = 0

Query: 19  LLLSIIIFNISTTIVVADLVQKTCKKCENNDPNINYNFCISSFHAHPGSDTA-DLRKLGA 78
           L++  ++ N   T     L++ +CKK    DP + Y+FC+ S   +P S TA  L  L  
Sbjct: 7   LVMFFLLLNCFAT--AQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLVF 66

Query: 79  ISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCLEVYSNAIDSMEEGKKAYKEKHY 138
           +S        ++    V+K+L+  + +  + L   DCLE+Y+ AIDS+ +     K + Y
Sbjct: 67  VSTKNAVSKTTSLKGMVDKILKEDKYEVERPLL--DCLELYTEAIDSLNQSLDTVKSRDY 126

Query: 139 NDANIKVSSVMDAARVCEDGF-REKEGVSSPLTKWNKDLFQLAAIALSIINM 189
             A + +S+ MDA   CE  F + K+ V SP TK N  LF +  I ++  +M
Sbjct: 127 KTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

BLAST of Clc06G00870 vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 3.7e-12
Identity = 35/130 (26.92%), Postives = 70/130 (53.85%), Query Frame = 0

Query: 56  FCISSFHAHPGSDTADLRKLGAISLSLIQKNVSNSFEYVEKLLQNKEIDSYKKLCLNDCL 115
           FC++S  + P + TA   KLG I+LS+   N S++  Y++  L+ K ++   +  L+DC 
Sbjct: 25  FCVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNLEPALEDTLDDCS 84

Query: 116 EVYSNAIDSMEEGKKAYKEKHYNDANIKVSSVMDAARVCEDGFREKEGVSSPLTKWNKDL 175
           + Y +A+  +++   A  +  + D +I +++ +     CE+   ++ G  + L + N +L
Sbjct: 85  KNYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRNTNL 144

Query: 176 FQLAAIALSI 186
            +L   AL I
Sbjct: 145 LKLCKDALLI 154

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460198.26.9e-8586.32PREDICTED: putative invertase inhibitor [Cucumis melo][more]
XP_038875624.13.4e-8488.42putative invertase inhibitor [Benincasa hispida][more]
XP_004145080.22.3e-8083.68putative invertase inhibitor [Cucumis sativus][more]
KAE8646717.13.3e-7983.51hypothetical protein Csa_005489 [Cucumis sativus][more]
KAA0039958.15.7e-7985.56putative invertase inhibitor [Cucumis melo var. makuwa] >TYK24545.1 putative inv... [more]
Match NameE-valueIdentityDescription
Q8GT419.2e-3242.69Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1[more]
A9YUH41.6e-3143.31Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1[more]
Q9FJR54.7e-1230.23Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1[more]
Q9LUV19.3e-0826.11Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1[more]
Q0J8J82.1e-0727.95Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P... [more]
Match NameE-valueIdentityDescription
A0A1S3CCF73.3e-8586.32putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103499083 PE=4 SV=1[more]
A0A0A0K9X71.1e-8083.68PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080360 PE=4 S... [more]
A0A5A7TAH12.7e-7985.56Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1KJ451.1e-6772.87putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494454 PE=4 SV... [more]
A0A6J1HKS59.1e-6771.28putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464448 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G38610.18.3e-2030.48Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46970.11.4e-1933.54Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46940.12.0e-1830.81Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46930.13.9e-1731.40Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT4G02250.13.7e-1226.92Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 115..135
NoneNo IPR availablePANTHERPTHR31080:SF226PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 30..188
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 30..188
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 33..185
e-value: 3.2E-33
score: 126.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 18..189
e-value: 2.5E-38
score: 129.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 36..185
e-value: 1.2E-30
score: 106.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 33..189
e-value: 1.2E-38
score: 134.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 36..188
IPR034088Pla a 1-likeCDDcd15795PMEI-Pla_a_1_likecoord: 42..188
e-value: 2.60628E-50
score: 157.527

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc06G00870.1Clc06G00870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004857 enzyme inhibitor activity