Clc06G00740 (gene) Watermelon (cordophanus) v2

Overview
NameClc06G00740
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationClcChr06: 661049 .. 663261 (-)
RNA-Seq ExpressionClc06G00740
SyntenyClc06G00740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTCTTTGACTTTGATTTTCTTGCTCTTTAAAGATTCAACTCTCCCTTGCAAGACTCACGAGAGCTTAATGTTGCCTTCAATCTATCCAACTACCTCTCTTTCTAATTCCACCGTCTTCAATGGAAGATGTCCACCAAAACCCTCTTCCTCCACCGCATCCACCGCCACCACCGACCGCGGTCGTGGACTTTCCGCCATACTTCCGATGCCCTATTTCCATGGAGCTCATGCAAGATCCAGTCACGATTTCCACCGGAGTTTCATTTGAGAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATCGTCAACTTTGATATCACTCCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAGGAGTCTTGCTCCTCATCGTCGTTGTTGCCGTCATCATCGATGGAGTATGATGATGTTGTGGCTGATCTCTTGAAGAAGATCGAGTTGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTACGGTTGCTCGTGGCAATGGATGATGTAGCTGCGGTCGGAGCGAAACCCATCCTTGTTCGAGAAAATGGGTTTGAAGTTCTTGTTGGAATAGTCACGCAAGTCGCTGTTTGTGAGAGCTCCGATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTTTGTTGCACACTAAACTCAAGAGTTTAATTTTATTTATTTTTTCTTTCTTAATGTTTAACTTGTAAGTGTGCATATTCAAACATAGATTTTTTTTTTTTTTTTTTAATAATACAATAATAGATGCATTAGAATATCTGAACTAGTTACATATTCTCATCACCCTCCTCATATTCAAACATCGTTTATAACCTCTTGATTGAAGATATATGCTTCAACTGATGATGTATAATCTGATTTCGTCAAAGATTTGGGTTTTATTTATATTCTTAATAAAAGGTTGTGTTTGAGAGTTTGAACTAGATGTAATTGGTAACTACTAATATTAGTCAAAAGAGAAAATGACATGAGAGAGAATTAACTTGAAATATAATTGAAGATTGCCATTATTTTAAGTATTGATGATGGGATAGATTATGATATATTCAATCTTTTATTCATATTCTTAACAGGTATTATCTCAATTCCCATTCTCCAAAACTGAAAAGCCATTCGAGTTGCTATCAAAACCAGAAGCCATTAAGTCAATGGCGATTATTTTACAAAGAGGAAGCATAGAAGGAAGGTTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGCTACGATTGGAACTCTTTAACAAAACACCATGCCATCGACATGTTCAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCTAAAACAATTGATTCATCTTCGATAATTACACAATGGTACCATATCGTCCACATGCTTATTTTCATTATATATTCCTTCATTTTAATTACTAAATCTTTCCTTTTTTTTAGTCGATACGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCAAAAAAAAGCCGTCTTCTATCGATTGAGGCCGGCGCTGTTTACGTCATGATTGACCTTCTTCCTGACTCGAACCGATCGAAGTGCGAGAAAATATTGCACATACTCAAACTTTTAAGTGAGTGTGCAGAGGGAAGGTCGGCCATGGGAGAGCATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATTTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAACTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGAGATTATTTGAAGTTGATAAATCAATAGAATATATGTGTTGAAGTGAATGAGAATTGGGTCTTGAAGTTTATTTTAAGGTAAAATTATAAGTTGGGTCTTCCAATTTTTAAGATTTTGTGTGTCTTAAGTGCTTGAACTTTCAAAAGTGCCTAGTAAAACTTTGAACTTTAAATGTTGCGTCTAATAGGTATTTAAACTTTAAAAATGATAATAAGTTCTTGAACTCTCAATTTTATGTCTA

mRNA sequence

GTTTTCTTTGACTTTGATTTTCTTGCTCTTTAAAGATTCAACTCTCCCTTGCAAGACTCACGAGAGCTTAATGTTGCCTTCAATCTATCCAACTACCTCTCTTTCTAATTCCACCGTCTTCAATGGAAGATGTCCACCAAAACCCTCTTCCTCCACCGCATCCACCGCCACCACCGACCGCGGTCGTGGACTTTCCGCCATACTTCCGATGCCCTATTTCCATGGAGCTCATGCAAGATCCAGTCACGATTTCCACCGGAGTTTCATTTGAGAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATCGTCAACTTTGATATCACTCCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAGGAGTCTTGCTCCTCATCGTCGTTGTTGCCGTCATCATCGATGGAGTATGATGATGTTGTGGCTGATCTCTTGAAGAAGATCGAGTTGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTACGGTTGCTCGTGGCAATGGATGATGTAGCTGCGGTCGGAGCGAAACCCATCCTTGTTCGAGAAAATGGGTTTGAAGTTCTTGTTGGAATAGTCACGCAAGTCGCTGTTTGTGAGAGCTCCGATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTCCCATTCTCCAAAACTGAAAAGCCATTCGAGTTGCTATCAAAACCAGAAGCCATTAAGTCAATGGCGATTATTTTACAAAGAGGAAGCATAGAAGGAAGGTTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGCTACGATTGGAACTCTTTAACAAAACACCATGCCATCGACATGTTCAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCTAAAACAATTGATTCATCTTCGATAATTACACAATGTCGATACGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCAAAAAAAAGCCGTCTTCTATCGATTGAGGCCGGCGCTGTTTACGTCATGATTGACCTTCTTCCTGACTCGAACCGATCGAAGTGCGAGAAAATATTGCACATACTCAAACTTTTAAGTGAGTGTGCAGAGGGAAGGTCGGCCATGGGAGAGCATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATTTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAACTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGAGATTATTTGAAGTTGATAAATCAATAGAATATATGTGTTGAAGTGAATGAGAATTGGGTCTTGAAGTTTATTTTAAGGTAAAATTATAAGTTGGGTCTTCCAATTTTTAAGATTTTGTGTGTCTTAAGTGCTTGAACTTTCAAAAGTGCCTAGTAAAACTTTGAACTTTAAATGTTGCGTCTAATAGGTATTTAAACTTTAAAAATGATAATAAGTTCTTGAACTCTCAATTTTATGTCTA

Coding sequence (CDS)

ATGGAAGATGTCCACCAAAACCCTCTTCCTCCACCGCATCCACCGCCACCACCGACCGCGGTCGTGGACTTTCCGCCATACTTCCGATGCCCTATTTCCATGGAGCTCATGCAAGATCCAGTCACGATTTCCACCGGAGTTTCATTTGAGAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATCGTCAACTTTGATATCACTCCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAGGAGTCTTGCTCCTCATCGTCGTTGTTGCCGTCATCATCGATGGAGTATGATGATGTTGTGGCTGATCTCTTGAAGAAGATCGAGTTGTCGCCGTTCAAGGCGAGTTCTTTGAAGAAACTACGGTTGCTCGTGGCAATGGATGATGTAGCTGCGGTCGGAGCGAAACCCATCCTTGTTCGAGAAAATGGGTTTGAAGTTCTTGTTGGAATAGTCACGCAAGTCGCTGTTTGTGAGAGCTCCGATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTCCCATTCTCCAAAACTGAAAAGCCATTCGAGTTGCTATCAAAACCAGAAGCCATTAAGTCAATGGCGATTATTTTACAAAGAGGAAGCATAGAAGGAAGGTTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGCTACGATTGGAACTCTTTAACAAAACACCATGCCATCGACATGTTCAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCTAAAACAATTGATTCATCTTCGATAATTACACAATGTCGATACGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCGTCAAAAAAAAGCCGTCTTCTATCGATTGAGGCCGGCGCTGTTTACGTCATGATTGACCTTCTTCCTGACTCGAACCGATCGAAGTGCGAGAAAATATTGCACATACTCAAACTTTTAAGTGAGTGTGCAGAGGGAAGGTCGGCCATGGGAGAGCATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATTTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAACTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGAGATTATTTGAAGTTGATAAATCAATAG

Protein sequence

MEDVHQNPLPPPHPPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLKKIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANFEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAIDMFKSILELASDEIIKKAKTIDSSSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ
Homology
BLAST of Clc06G00740 vs. NCBI nr
Match: XP_038875803.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 817.8 bits (2111), Expect = 4.7e-233
Identity = 415/450 (92.22%), Postives = 430/450 (95.56%), Query Frame = 0

Query: 1   MEDVHQNPLPPPHPPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MED+HQN LPPP PPPP   VVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLKK 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWK KES SSSS  PSSSM+YDDVVADLLK 
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLKN 120

Query: 121 IELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANFE 180
           IE +PFKASSLKKLRLL+AMDDVAAVGAKP+ VR NGF+VLVGI+TQVA+CES+DFANFE
Sbjct: 121 IESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFE 180

Query: 181 ACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGY 240
           ACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAI+LQRGS+EGRFYAMEMLQQISKK Y
Sbjct: 181 ACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEY 240

Query: 241 DWNSLTKHHAIDMFKSILELASDEIIKKAKTIDSS--SIITQCRYGTLFSSALELLIEIM 300
           DWNSLTKHH ID+FKSILELASDEIIKKA TI ++  SIITQCRYGTLFSSALELLIEIM
Sbjct: 241 DWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIM 300

Query: 301 ESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360
           ESSKKSRLLSIEAGAV VMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT
Sbjct: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360

Query: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKA 420
           VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKA
Sbjct: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKA 420

Query: 421 MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 449
           MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ
Sbjct: 421 MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 450

BLAST of Clc06G00740 vs. NCBI nr
Match: XP_008460073.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 804.7 bits (2077), Expect = 4.1e-229
Identity = 413/452 (91.37%), Postives = 430/452 (95.13%), Query Frame = 0

Query: 1   MEDVHQNPLPPPH-PPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MED+HQ+P PP H P PPPTA VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSS+SLLPSSSMEY DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 KIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANF 180
            IE SPFKASSLKKLRLL+A+DDVAAVGAKP+ VR NGFE+LV IVTQVA+CE SDFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHAIDMFKSILELASDEIIKKA---KTIDSSSIITQCRYGTLFSSALELLIE 300
           YDWNSLTKH  ID+FKSILELASDEIIKKA   KT+DSS+ ITQCRYGTLFSSALELLIE
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSST-ITQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAV VMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKE 420
           ATVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGILNGDG SSTKE
Sbjct: 361 ATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 449
           KAMK+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 KAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of Clc06G00740 vs. NCBI nr
Match: XP_004145072.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical protein Csa_004999 [Cucumis sativus])

HSP 1 Score: 800.0 bits (2065), Expect = 1.0e-227
Identity = 414/453 (91.39%), Postives = 429/453 (94.70%), Query Frame = 0

Query: 1   MEDVHQNPLPPPHPPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MED+HQNP PPPH P P T  VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNPFPPPHAPTPTT--VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSS-SSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSS SSLLPSSSMEY DVVADLL
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLL 120

Query: 121 KKIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFAN 180
           K IE SPFKASSLKKLRLL+A+DDVAAVGAKP+ VR NGFEVL+GIVTQVA+CESSDFAN
Sbjct: 121 KNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAI+LQRGSIEGRFYAMEMLQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240

Query: 241 GYDWNSLTKHHAIDMFKSILELASDEIIKKAKTIDS--SSIITQCRYGTLFSSALELLIE 300
           GYDWNSLTKH  ID+FKSILELASDEIIKKA  I +  SSI+T CRYGTLFSSALELLIE
Sbjct: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAV VMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL-NGDGRSSTK 420
           ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL NGDGRSSTK
Sbjct: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 449
           EKA K+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of Clc06G00740 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761.5 bits (1965), Expect = 4.0e-216
Identity = 396/452 (87.61%), Postives = 415/452 (91.81%), Query Frame = 0

Query: 1   MEDVHQNPLPPPHPPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MED+ QN LPP  P PPP AV D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--SSSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  SSS     SMEYDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVA-LL 120

Query: 121 KKIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFAN 180
           K IELSPFKASSLKKLRLL+A+DDVAAVGAKPI V+ NGFEVLVG+VTQVA+CESS+FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEK FELLS+PEAIKSMAI+LQRGSIEGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 GYDWNSLTKHHAIDMFKSILELASDEIIKKAKTI--DSSSIITQCRYGTLFSSALELLIE 300
           GYD+NSLTKHHAID+FKSILELASDEIIKKA T+    SSI TQCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAV +MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR-SSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GILNGDGR SSTK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 448
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of Clc06G00740 vs. NCBI nr
Match: KAG6574816.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 756.5 bits (1952), Expect = 1.3e-214
Identity = 391/452 (86.50%), Postives = 414/452 (91.59%), Query Frame = 0

Query: 1   MEDVHQNPLPPPHPPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MED+ QN LPPP P PPP  V D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--SSSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLI+SWKM ESCS  SSS     SMEYDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVA-LL 120

Query: 121 KKIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFAN 180
           K IELSPFKASSLKKLRLL+A+DDVAAVGAKPI V+ NGFEVLVG+VTQ+A+CESS+FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSK EK FELL++PEAIKSMAI+LQRGSIEGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 GYDWNSLTKHHAIDMFKSILELASDEIIKKAKTI--DSSSIITQCRYGTLFSSALELLIE 300
           GYD+NSL+KHHAID+FKSILELASDEIIKKA T+    SSI TQCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAV +MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR-SSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GILNGDGR SSTK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 448
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of Clc06G00740 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.8e-47
Identity = 135/427 (31.62%), Postives = 213/427 (49.88%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ PP+F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 82  YTLKRLILSWKMKESCSSSSLLPS-----SSMEYDDVVADLLKKIELSPFKASSLKKLRL 141
           +TL+RLI SW    +      +P+        E + ++ D     E    +   LK+LR 
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHE---NQVKCLKRLRQ 129

Query: 142 LVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANFEACEEALGVLSQFPFSK 201
           +V+ +       K  L      E L  IV       S+D  N    +EAL +L     S+
Sbjct: 130 IVSEN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSE 189

Query: 202 TEKPFELLSKPE--AIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAIDMF 261
           T     L +K +   +KS+  I+QRG  E R YA  +L+ I +      S+T     ++F
Sbjct: 190 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVF 249

Query: 262 KSILELASDEIIKKAKTIDSSSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAV 321
             ++++  D I +KA       ++  C +G                  ++R  ++EAG +
Sbjct: 250 TEVVQILDDRISQKATKAAMHILVNICPWG------------------RNRHKAVEAGVI 309

Query: 322 YVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNTA 381
            V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S TA
Sbjct: 310 SVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTA 369

Query: 382 TKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGSFW 437
           +   +++L  +  +  +  +L EM+  G V KL  +L       TKEKA +++KLH   W
Sbjct: 370 SDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 402

BLAST of Clc06G00740 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-42
Identity = 128/451 (28.38%), Postives = 218/451 (48.34%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ P +F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEY-DDVVADLLKKIELSPF-KASSLKKLRLLVA 141
           +TL+RLI SW    +      +P+         +  L+K+   S   +   LK+LR +V+
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 142 MDDVAAVGAKPILVRENGFEVLVGIVTQVA-----------------VCESSDFAN-FEA 201
            +       K  L      E L  IV+                    +C+S+   N F++
Sbjct: 125 ENTT----NKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 184

Query: 202 ----CEEALGVLSQFPFSKTEKPFELLSK--PEAIKSMAIILQRGSIEGRFYAMEMLQQI 261
                +EAL VL     S+T     L +K     +K++  I+QRG  E R YA  +L+++
Sbjct: 185 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 244

Query: 262 SKKGYDWNSLTKHHAIDMFKSILELASDEIIKKAKTIDSSSIITQCRYGTLFSSALELLI 321
            +       +      ++F  ++++  D+I  KA       ++  C +G           
Sbjct: 245 LEVADPMQIILLER--ELFGEVIQILHDQISHKATRSAMQILVITCPWG----------- 304

Query: 322 EIMESSKKSRLLSIEAGAVYVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGE 381
                  ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+    
Sbjct: 305 -------RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLN 364

Query: 382 HIMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDG 441
           H   IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L    
Sbjct: 365 HGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSC 424

BLAST of Clc06G00740 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 9.4e-35
Identity = 120/427 (28.10%), Postives = 202/427 (47.31%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           +  P +FRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T   + +F + PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEYDDV-VADLLKKIELSP-------FKASSLKK 141
           +TL+RLI  W +    +    +P+     D   V  LL +             +A++L++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 142 LRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANFEACEEALGVLSQFP 201
           LR      D   V    ++   N  E+L+ I+        S+  + E   E+L +L   P
Sbjct: 132 LRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLALLVMLP 191

Query: 202 FSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQIS--KKGYDWNSLTKHHAI 261
            ++  +   + S P  ++ +  +L   SIE R  A  +++ +S   K  D    +  ++ 
Sbjct: 192 ITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKG-SISNSE 251

Query: 262 DMFKSILELASDEI-IKKAKTIDSSSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIE 321
            +F+ +L+L  + I  ++A  I   ++   C                  S K +R ++I 
Sbjct: 252 SVFEGVLDLLRNPISSRRALKIGIKTLFALC------------------SVKSTRHIAIT 311

Query: 322 AGAVYVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNT 381
           AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  + +S+ 
Sbjct: 312 AGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDR 371

Query: 382 ATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGSF 436
           AT+     L  +C     ER  EE  G G V +LL ++  +     K+KA K++KL    
Sbjct: 372 ATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDS 406

BLAST of Clc06G00740 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.1e-34
Identity = 125/448 (27.90%), Postives = 216/448 (48.21%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 81
           ++ P YF CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 8   IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 67

Query: 82  NYTLKRLILSWKMKESCS-----SSSLLPSSSMEYDDVVADLLKKIELSPFKASSLKKLR 141
           N+ L+RLI  W ++         S+  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 68  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 127

Query: 142 LLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSD---FANFEACEEALGVLSQF 201
           +L AMD     G    L+ E G  V   ++  V  C S D       +  +E+L +L   
Sbjct: 128 VL-AMD-----GNNRRLMCECG--VHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLI 187

Query: 202 PFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAID 261
                +    L+     ++S+  +L +     + Y + +L+ +++  Y  + + +    +
Sbjct: 188 GIPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPE 247

Query: 262 MFKSILELASDEIIKKAKT-----------------------IDSSSIITQCRYGTLFSS 321
           +FK I+    D +    +T                       +D S +I Q       ++
Sbjct: 248 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQA-----VTA 307

Query: 322 ALELLIEIMESSKKSRLLSIEAGAVYVMIDLLPDSNRSK--CEKILHILKLLSECAEGRS 381
           AL +L+E   S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+
Sbjct: 308 ALMILLE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRA 367

Query: 382 AMGEHIMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL 435
            +  H  GIA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L
Sbjct: 368 EILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVL 427

BLAST of Clc06G00740 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.6e-29
Identity = 119/421 (28.27%), Postives = 198/421 (47.03%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           +  P +FRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F + PN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDFTLIPN 71

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEYDDV-VADLLKKIELSP-------FKASSLKK 141
           +TL+RLI  W +    +    +P+     D + V  LL +             +A+++++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRR 131

Query: 142 LRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANFEACEEALGVLSQFP 201
           LR L    +   V    ++   N  E+LV I+   A  E++  ++ E   E+L +L    
Sbjct: 132 LRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSESLALLVLLH 191

Query: 202 FSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQI--SKKGYDWNSLTKHHAI 261
            ++TE    + S P  +  M  +L   SIE R  A  +++ +    K  D   L    + 
Sbjct: 192 MTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDL-KLIISGSD 251

Query: 262 DMFKSILELASDEI-IKKAKTIDSSSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIE 321
            +F+ +L+L  + I  ++A  I   +I   C                    K++R L+I 
Sbjct: 252 SIFEGVLDLLKNPISSRRALKIGIKAIFALCLV------------------KQTRHLAIS 311

Query: 322 AGAVYVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNT 381
           AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  + +S+ 
Sbjct: 312 AGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDR 371

Query: 382 ATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGSF 430
           AT+     L  +C     ER  +E    G V +LL ++  D     K KA  ++KL    
Sbjct: 372 ATEYAAGALLALCT--AEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDS 402

BLAST of Clc06G00740 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.0e-229
Identity = 413/452 (91.37%), Postives = 430/452 (95.13%), Query Frame = 0

Query: 1   MEDVHQNPLPPPH-PPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MED+HQ+P PP H P PPPTA VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSS+SLLPSSSMEY DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 KIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANF 180
            IE SPFKASSLKKLRLL+A+DDVAAVGAKP+ VR NGFE+LV IVTQVA+CE SDFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHAIDMFKSILELASDEIIKKA---KTIDSSSIITQCRYGTLFSSALELLIE 300
           YDWNSLTKH  ID+FKSILELASDEIIKKA   KT+DSS+ ITQCRYGTLFSSALELLIE
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSST-ITQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAV VMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKE 420
           ATVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGILNGDG SSTKE
Sbjct: 361 ATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 449
           KAMK+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 KAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of Clc06G00740 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.9e-228
Identity = 414/453 (91.39%), Postives = 429/453 (94.70%), Query Frame = 0

Query: 1   MEDVHQNPLPPPHPPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MED+HQNP PPPH P P T  VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNPFPPPHAPTPTT--VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSS-SSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSS SSLLPSSSMEY DVVADLL
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLL 120

Query: 121 KKIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFAN 180
           K IE SPFKASSLKKLRLL+A+DDVAAVGAKP+ VR NGFEVL+GIVTQVA+CESSDFAN
Sbjct: 121 KNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAI+LQRGSIEGRFYAMEMLQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240

Query: 241 GYDWNSLTKHHAIDMFKSILELASDEIIKKAKTIDS--SSIITQCRYGTLFSSALELLIE 300
           GYDWNSLTKH  ID+FKSILELASDEIIKKA  I +  SSI+T CRYGTLFSSALELLIE
Sbjct: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAV VMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL-NGDGRSSTK 420
           ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL NGDGRSSTK
Sbjct: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 449
           EKA K+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of Clc06G00740 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 9.0e-214
Identity = 389/452 (86.06%), Postives = 412/452 (91.15%), Query Frame = 0

Query: 1   MEDVHQNPLPPPHPPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MED+ QN LPPP P PPP  V D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--SSSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  SSS     SMEYDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVA-LL 120

Query: 121 KKIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFAN 180
           K IELSPFKASSLKKLRLL+A+DDV AVGAKPI V+ NGFEVLVG+VTQ+A+CESS+FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSK EK FELL++PEAIKSMAI+LQRGSIEGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 GYDWNSLTKHHAIDMFKSILELASDEIIKKAKTI--DSSSIITQCRYGTLFSSALELLIE 300
           GYD+NSL+KHHAID+FKSILELASDEIIKKA T+    SSI T CRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAV +MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR-SSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEM+GCGTVKKL+GILNGDGR SSTK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 448
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of Clc06G00740 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.2e-210
Identity = 389/452 (86.06%), Postives = 410/452 (90.71%), Query Frame = 0

Query: 1   MEDVHQNPLPPPHPPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MED+ QN LPPP PP    A  D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPP----AAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--SSSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  SSS     S+EYDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVA-LL 120

Query: 121 KKIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFAN 180
           K IELSPFKASSLKKLRLL+A+DDVAAV AK I V+ NGFEVLVG+VTQVA+CESS+FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEK FELLSK EAIKSM I+LQRGSIEGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKK 240

Query: 241 GYDWNSLTKHHAIDMFKSILELASDEIIKKAKTIDS--SSIITQCRYGTLFSSALELLIE 300
           GYD+NSL+KHHAID+FKSILELASDEIIKKA TI +  SSI TQCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGA+ +MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR-SSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GILNGDGR SSTK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 448
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

BLAST of Clc06G00740 vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 4.2e-203
Identity = 375/451 (83.15%), Postives = 392/451 (86.92%), Query Frame = 0

Query: 1   MEDVHQNPLPPPH-PPPPPTAVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MED+HQ+P PP H P PPPTA VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSS+SLLPSSSMEY DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 KIELSPFKASSLKKLRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANF 180
            IE SPFKASSLKKLRLL+A+DDVAAVGAKP+ VR NGFE+LV IVTQVA+CE SDF NF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFTNF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHAIDMFKSILELASDEIIKKAKTIDS--SSIITQCRYGTLFSSALELLIEI 300
           YDWNSLTKH  ID+FKSILELASDEIIKKA  I +  SS ITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVYVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360
           MESSKKSRLLSIEAGAV VMIDLLPDSNRSKCEKIL+ILKLLSECAEG            
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG------------ 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEK 420
                                   +YHPSERVLEEMMGCGTVKKLLGILNGDG SSTKEK
Sbjct: 361 ------------------------SYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 415

Query: 421 AMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 449
           AMK+MKLHGSFWR+FPCFPYE+RDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRKFPCFPYEYRDYLKLINQ 415

BLAST of Clc06G00740 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 191.0 bits (484), Expect = 2.0e-48
Identity = 135/427 (31.62%), Postives = 213/427 (49.88%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ PP+F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 82  YTLKRLILSWKMKESCSSSSLLPS-----SSMEYDDVVADLLKKIELSPFKASSLKKLRL 141
           +TL+RLI SW    +      +P+        E + ++ D     E    +   LK+LR 
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHE---NQVKCLKRLRQ 129

Query: 142 LVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANFEACEEALGVLSQFPFSK 201
           +V+ +       K  L      E L  IV       S+D  N    +EAL +L     S+
Sbjct: 130 IVSEN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSE 189

Query: 202 TEKPFELLSKPE--AIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAIDMF 261
           T     L +K +   +KS+  I+QRG  E R YA  +L+ I +      S+T     ++F
Sbjct: 190 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVF 249

Query: 262 KSILELASDEIIKKAKTIDSSSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAV 321
             ++++  D I +KA       ++  C +G                  ++R  ++EAG +
Sbjct: 250 TEVVQILDDRISQKATKAAMHILVNICPWG------------------RNRHKAVEAGVI 309

Query: 322 YVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNTA 381
            V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S TA
Sbjct: 310 SVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTA 369

Query: 382 TKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGSFW 437
           +   +++L  +  +  +  +L EM+  G V KL  +L       TKEKA +++KLH   W
Sbjct: 370 SDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 402

BLAST of Clc06G00740 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 175.6 bits (444), Expect = 8.7e-44
Identity = 128/451 (28.38%), Postives = 218/451 (48.34%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ P +F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEY-DDVVADLLKKIELSPF-KASSLKKLRLLVA 141
           +TL+RLI SW    +      +P+         +  L+K+   S   +   LK+LR +V+
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 142 MDDVAAVGAKPILVRENGFEVLVGIVTQVA-----------------VCESSDFAN-FEA 201
            +       K  L      E L  IV+                    +C+S+   N F++
Sbjct: 125 ENTT----NKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 184

Query: 202 ----CEEALGVLSQFPFSKTEKPFELLSK--PEAIKSMAIILQRGSIEGRFYAMEMLQQI 261
                +EAL VL     S+T     L +K     +K++  I+QRG  E R YA  +L+++
Sbjct: 185 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 244

Query: 262 SKKGYDWNSLTKHHAIDMFKSILELASDEIIKKAKTIDSSSIITQCRYGTLFSSALELLI 321
            +       +      ++F  ++++  D+I  KA       ++  C +G           
Sbjct: 245 LEVADPMQIILLER--ELFGEVIQILHDQISHKATRSAMQILVITCPWG----------- 304

Query: 322 EIMESSKKSRLLSIEAGAVYVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGE 381
                  ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+    
Sbjct: 305 -------RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLN 364

Query: 382 HIMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDG 441
           H   IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L    
Sbjct: 365 HGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSC 424

BLAST of Clc06G00740 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 149.4 bits (376), Expect = 6.7e-36
Identity = 120/427 (28.10%), Postives = 202/427 (47.31%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           +  P +FRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T   + +F + PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEYDDV-VADLLKKIELSP-------FKASSLKK 141
           +TL+RLI  W +    +    +P+     D   V  LL +             +A++L++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 142 LRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANFEACEEALGVLSQFP 201
           LR      D   V    ++   N  E+L+ I+        S+  + E   E+L +L   P
Sbjct: 132 LRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLALLVMLP 191

Query: 202 FSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQIS--KKGYDWNSLTKHHAI 261
            ++  +   + S P  ++ +  +L   SIE R  A  +++ +S   K  D    +  ++ 
Sbjct: 192 ITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKG-SISNSE 251

Query: 262 DMFKSILELASDEI-IKKAKTIDSSSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIE 321
            +F+ +L+L  + I  ++A  I   ++   C                  S K +R ++I 
Sbjct: 252 SVFEGVLDLLRNPISSRRALKIGIKTLFALC------------------SVKSTRHIAIT 311

Query: 322 AGAVYVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNT 381
           AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  + +S+ 
Sbjct: 312 AGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDR 371

Query: 382 ATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGSF 436
           AT+     L  +C     ER  EE  G G V +LL ++  +     K+KA K++KL    
Sbjct: 372 ATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDS 406

BLAST of Clc06G00740 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-35
Identity = 125/448 (27.90%), Postives = 216/448 (48.21%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 81
           ++ P YF CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 22  IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 81

Query: 82  NYTLKRLILSWKMKESCS-----SSSLLPSSSMEYDDVVADLLKKIELSPFKASSLKKLR 141
           N+ L+RLI  W ++         S+  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 82  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 141

Query: 142 LLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSD---FANFEACEEALGVLSQF 201
           +L AMD     G    L+ E G  V   ++  V  C S D       +  +E+L +L   
Sbjct: 142 VL-AMD-----GNNRRLMCECG--VHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLI 201

Query: 202 PFSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAID 261
                +    L+     ++S+  +L +     + Y + +L+ +++  Y  + + +    +
Sbjct: 202 GIPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPE 261

Query: 262 MFKSILELASDEIIKKAKT-----------------------IDSSSIITQCRYGTLFSS 321
           +FK I+    D +    +T                       +D S +I Q       ++
Sbjct: 262 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQA-----VTA 321

Query: 322 ALELLIEIMESSKKSRLLSIEAGAVYVMIDLLPDSNRSK--CEKILHILKLLSECAEGRS 381
           AL +L+E   S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+
Sbjct: 322 ALMILLE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRA 381

Query: 382 AMGEHIMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL 435
            +  H  GIA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L
Sbjct: 382 EILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVL 441

BLAST of Clc06G00740 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-30
Identity = 119/421 (28.27%), Postives = 198/421 (47.03%), Query Frame = 0

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           +  P +FRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F + PN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDFTLIPN 71

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEYDDV-VADLLKKIELSP-------FKASSLKK 141
           +TL+RLI  W +    +    +P+     D + V  LL +             +A+++++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRR 131

Query: 142 LRLLVAMDDVAAVGAKPILVRENGFEVLVGIVTQVAVCESSDFANFEACEEALGVLSQFP 201
           LR L    +   V    ++   N  E+LV I+   A  E++  ++ E   E+L +L    
Sbjct: 132 LRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSESLALLVLLH 191

Query: 202 FSKTEKPFELLSKPEAIKSMAIILQRGSIEGRFYAMEMLQQI--SKKGYDWNSLTKHHAI 261
            ++TE    + S P  +  M  +L   SIE R  A  +++ +    K  D   L    + 
Sbjct: 192 MTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDL-KLIISGSD 251

Query: 262 DMFKSILELASDEI-IKKAKTIDSSSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIE 321
            +F+ +L+L  + I  ++A  I   +I   C                    K++R L+I 
Sbjct: 252 SIFEGVLDLLKNPISSRRALKIGIKAIFALCLV------------------KQTRHLAIS 311

Query: 322 AGAVYVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISNT 381
           AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  + +S+ 
Sbjct: 312 AGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDR 371

Query: 382 ATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGSF 430
           AT+     L  +C     ER  +E    G V +LL ++  D     K KA  ++KL    
Sbjct: 372 ATEYAAGALLALCT--AEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDS 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875803.14.7e-23392.22E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida][more]
XP_008460073.14.1e-22991.37PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo][more]
XP_004145072.21.0e-22791.39E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical pro... [more]
XP_023549278.14.0e-21687.61E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo][more]
KAG6574816.11.3e-21486.50E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q84TG32.8e-4731.62E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC61.2e-4228.38E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9LT799.4e-3528.10U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9SF152.1e-3427.90E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9FXA41.6e-2928.27U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A1S3CC542.0e-22991.37RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ... [more]
A0A0A0KBP64.9e-22891.39RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE... [more]
A0A6J1H6I89.0e-21486.06RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075... [more]
A0A6J1KWQ81.2e-21086.06RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P... [more]
A0A5D3DLY24.2e-20383.15RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT2G35930.12.0e-4831.62plant U-box 23 [more]
AT3G52450.18.7e-4428.38plant U-box 22 [more]
AT3G19380.16.7e-3628.10plant U-box 25 [more]
AT3G11840.11.5e-3527.90plant U-box 24 [more]
AT1G49780.11.1e-3028.27plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 27..91
e-value: 9.3E-24
score: 94.9
IPR003613U box domainPFAMPF04564U-boxcoord: 25..95
e-value: 9.3E-17
score: 61.0
IPR003613U box domainPROSITEPS51698U_BOXcoord: 23..98
score: 27.193447
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 102..428
e-value: 7.5E-10
score: 39.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 19..101
e-value: 2.3E-23
score: 84.0
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 24..447
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 24..447
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 28..71
e-value: 5.14599E-21
score: 83.8222
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 19..96
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 176..421

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc06G00740.1Clc06G00740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity