Clc05G25310 (gene) Watermelon (cordophanus) v2

BLAST of Clc05G25310 vs. NCBI nr
Match: XP_038891534.1 (uncharacterized protein LOC120080925 [Benincasa hispida] >XP_038891535.1 uncharacterized protein LOC120080925 [Benincasa hispida])

HSP 1 Score: 714.1 bits (1842), Expect = 6.6e-202
Identity = 364/379 (96.04%), Postives = 372/379 (98.15%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE
Sbjct: 1   MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKLQ+YLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF
Sbjct: 61  MSGGLSSHTQQVLHKLQKYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAG+YQYLAKE
Sbjct: 121 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGIYQYLAKE 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP+IQHCLPSERPPEVIPSTSA MSL+CLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE
Sbjct: 181 ILPSIQHCLPSERPPEVIPSTSAAMSLICLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNES NHL+IISGE  DMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR
Sbjct: 241 LLNESVNHLRIISGEWKDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           YALTDVKKEMPRENSWK+VFG EIDIVAE LRKFERENEIVWH KIPSRDELPTPKGTQI
Sbjct: 301 YALTDVKKEMPRENSWKIVFGIEIDIVAEMLRKFERENEIVWHNKIPSRDELPTPKGTQI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           VKSIPYHP+KWERELAFKI
Sbjct: 361 VKSIPYHPKKWERELAFKI 379

BLAST of Clc05G25310 vs. NCBI nr
Match: XP_008446562.1 (PREDICTED: uncharacterized protein LOC103489257 [Cucumis melo])

HSP 1 Score: 694.9 bits (1792), Expect = 4.2e-196
Identity = 354/379 (93.40%), Postives = 365/379 (96.31%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDP+KLKTKMCVFEDKF+ARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE
Sbjct: 1   MLLQFSDPSKLKTKMCVFEDKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKLQQYLPLLENF+SQVDM+DYNLRIVQWTSDLKIRWSSALSSS LF
Sbjct: 61  MSGGLSSHTQQVLHKLQQYLPLLENFISQVDMLDYNLRIVQWTSDLKIRWSSALSSSLLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEVGM LSLYGA LRQRA EVISEDQVQSATFFREAAGVYQYLA+E
Sbjct: 121 HLRGPKFFQIDNLRFEVGMMLSLYGAFLRQRALEVISEDQVQSATFFREAAGVYQYLAEE 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP IQHCLPSERPPEVIPSTSA MSL+CLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE
Sbjct: 181 ILPTIQHCLPSERPPEVIPSTSAAMSLICLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNESANHL I SGE  DMSSNFLEFLSAFRALHEL SRKNLAKELMSGGQ+GVAIGVLR
Sbjct: 241 LLNESANHLHISSGESKDMSSNFLEFLSAFRALHELMSRKNLAKELMSGGQSGVAIGVLR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           YALTDVKKEMPRENSWKLVFG EIDIV ETLRKFERENEIVWHKKIPSRDELPTP GTQI
Sbjct: 301 YALTDVKKEMPRENSWKLVFGIEIDIVTETLRKFERENEIVWHKKIPSRDELPTPVGTQI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           VKSIPY+P+KWER+LAFKI
Sbjct: 361 VKSIPYNPKKWERDLAFKI 379

BLAST of Clc05G25310 vs. NCBI nr
Match: XP_011655761.1 (uncharacterized protein LOC101214130 isoform X1 [Cucumis sativus] >KGN52053.1 hypothetical protein Csa_009245 [Cucumis sativus])

HSP 1 Score: 691.0 bits (1782), Expect = 6.0e-195
Identity = 354/379 (93.40%), Postives = 364/379 (96.04%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDPAKLKTKMCVFEDKF+ARDSGTLEQLKE+TSRRRVIEESINETSSITDAIARE
Sbjct: 1   MLLQFSDPAKLKTKMCVFEDKFIARDSGTLEQLKEITSRRRVIEESINETSSITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF
Sbjct: 61  MSGGLSSHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEVGMTLSLYGA LRQRA E+ISEDQVQSATFFREA+GVYQYLA+E
Sbjct: 121 HLRGPKFFQIDNLRFEVGMTLSLYGAFLRQRALEIISEDQVQSATFFREASGVYQYLAEE 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP IQHCLPSERPPEVIPSTSA MSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE
Sbjct: 181 ILPTIQHCLPSERPPEVIPSTSAAMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNESAN+L I  GEC DMSSNFLEFLSAFRALHEL SRKNLAKELMS GQ GVAIGVLR
Sbjct: 241 LLNESANYLHISFGECKDMSSNFLEFLSAFRALHELMSRKNLAKELMSSGQVGVAIGVLR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           YALTDVKKEMPRENSWKLVFG EIDIVAETLRKFERENEIVWHKKI  RDELPTP GT+I
Sbjct: 301 YALTDVKKEMPRENSWKLVFGIEIDIVAETLRKFERENEIVWHKKIALRDELPTPIGTRI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           VKSIPY+P+KWERELAFKI
Sbjct: 361 VKSIPYNPKKWERELAFKI 379

BLAST of Clc05G25310 vs. NCBI nr
Match: XP_023541232.1 (uncharacterized protein LOC111801459 [Cucurbita pepo subsp. pepo] >XP_023541233.1 uncharacterized protein LOC111801459 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 671.4 bits (1731), Expect = 4.9e-189
Identity = 344/379 (90.77%), Postives = 363/379 (95.78%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDPAKLKTKMCVFEDKF+ARDS TLEQLKEMTSRRRVIEESINETSSITDAIARE
Sbjct: 1   MLLQFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRRVIEESINETSSITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKL QYLPLLENFVSQVD++ YNL+IVQWTSDLKIRWSSALSSSSLF
Sbjct: 61  MSGGLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEV MTL LYGAVLRQRA EVISEDQVQSATFFREAAGVYQYLAKE
Sbjct: 121 HLRGPKFFQIDNLRFEVAMTLFLYGAVLRQRALEVISEDQVQSATFFREAAGVYQYLAKE 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP+IQHC+PSERPPEVIPSTS  MSLVCLAEAQAVT+MRAEEKG+I SLLAKLHYGIV+
Sbjct: 181 ILPSIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVD 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNESANHLQI+SGE    SS+FLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIG+LR
Sbjct: 241 LLNESANHLQILSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGILR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           +ALTDVKK+MPRENSW+LVFG EID VAETLRKFERENEIVWHKKIPSRDELPTPKG+QI
Sbjct: 301 HALTDVKKDMPRENSWRLVFGTEIDSVAETLRKFERENEIVWHKKIPSRDELPTPKGSQI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           VKSIPY P+KWERELAFK+
Sbjct: 361 VKSIPYCPKKWERELAFKM 375

BLAST of Clc05G25310 vs. NCBI nr
Match: XP_022944843.1 (uncharacterized protein LOC111449253 [Cucurbita moschata] >XP_022944844.1 uncharacterized protein LOC111449253 [Cucurbita moschata])

HSP 1 Score: 666.8 bits (1719), Expect = 1.2e-187
Identity = 341/379 (89.97%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDPAKLKTKMCVFEDKF+ARDS TLEQLKEMTSRRRVIEESINETSSITDAIARE
Sbjct: 1   MLLQFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRRVIEESINETSSITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKL QYLPLLENFVSQVD++ YNL+IVQWTSDLKIRWSSALSSSSLF
Sbjct: 61  MSGGLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEV MTL LYGAVLRQRA EVISEDQVQSATFFREAAGVYQYLAK+
Sbjct: 121 HLRGPKFFQIDNLRFEVAMTLFLYGAVLRQRALEVISEDQVQSATFFREAAGVYQYLAKD 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP+IQHC+PSERPPEVIPSTS  MSLVCLAEAQAVT+MRAEEKG+I SLLAKLHYGIV+
Sbjct: 181 ILPSIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVD 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNESANHL+I+SGE    SS+FLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIG+LR
Sbjct: 241 LLNESANHLRILSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGILR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           +ALTDVKK+MPRENSW+LVFG EID VAETLRKFERENEIVWHKKIPSRDELPTPKG+QI
Sbjct: 301 HALTDVKKDMPRENSWRLVFGTEIDSVAETLRKFERENEIVWHKKIPSRDELPTPKGSQI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           V SIPY P+KWERELAFK+
Sbjct: 361 VSSIPYCPKKWERELAFKM 375

BLAST of Clc05G25310 vs. ExPASy Swiss-Prot
Match: Q9W6C5 (Programmed cell death 6-interacting protein OS=Xenopus laevis OX=8355 GN=pdcd6ip PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.2e-07
Identity = 62/299 (20.74%), Postives = 131/299 (43.81%), Query Frame = 0

Query: 101 LHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLFHLRGPKFFQIDN 160
           L K +  L  +  +  Q+  ++      +    L   W  A    S+F   G     + +
Sbjct: 57  LDKHETSLETVMRYYDQLCSVEPKFPFTESQLCLTFTWKDAFDKGSIF--GGSVKLALPS 116

Query: 161 LRFEVGMTLSLYGAVLRQRASE--VISEDQVQSAT-FFREAAGVYQYLAKEILPAIQHCL 220
           L +E    L   GA+  Q ASE  + +++ +++A+ F++ A+G + ++   +L ++    
Sbjct: 117 LGYEKTCVLFNIGALASQIASEQNLDNDEALKAASKFYQLASGAFSHIKDTVLSSLNR-- 176

Query: 221 PSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLNESANHL 280
             +   ++ P T   +SL+ LA+AQ V  ++A       +++AK       L N++A++ 
Sbjct: 177 --DPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAVIAK-------LANQAADYY 236

Query: 281 QIISGEC---NDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLRYALTDV 340
                +C   + +S      L+A   + +  +  + +       + G  IG L++A   V
Sbjct: 237 GDAFKQCQYKDTLSKEVFPILAAKHCIMQAHAEYHQSVLAKQQKKFGEEIGRLQHASDLV 296

Query: 341 KKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQIVKSIP 394
           K    R + +  V     D +   L   +++N+ ++H ++P   +L       +VKS P
Sbjct: 297 KTVSSRYDEYVNVKDL-ADKINRALTAAKKDNDFIYHDRVPDLKDLDPVGKASLVKSTP 341

BLAST of Clc05G25310 vs. ExPASy Swiss-Prot
Match: Q8WUM4 (Programmed cell death 6-interacting protein OS=Homo sapiens OX=9606 GN=PDCD6IP PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.2e-05
Identity = 67/314 (21.34%), Postives = 127/314 (40.45%), Query Frame = 0

Query: 86  AREMSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSS 145
           A E+S    +   + L K +  L  L  +  Q+  I+      +    L   W  A    
Sbjct: 43  AEELSKLRRAAVGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKG 102

Query: 146 SLFHLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYL 205
           SLF   G     + +L +E    L    A+    AS++ +E  + +    + AA  YQ+ 
Sbjct: 103 SLF--GGSVKLALASLGYEKSCVLFNCAAL----ASQIAAEQNLDNDEGLKIAAKHYQFA 162

Query: 206 AKEIL---PAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKL 265
           +   L     +   L  E   ++ P T   +SL+ LA+AQ V  ++A       +++AK 
Sbjct: 163 SGAFLHIKETVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAK- 222

Query: 266 HYGIVELLNESANHLQIISGECNDMSSNFLEFLSAFRALH-ELKSRKNLAKELMSGGQA- 325
                 L N++A++      +C    +   E      A H  +++     + +++  Q  
Sbjct: 223 ------LANQAADYFGDAFKQCQYKDTLPKEVFPVLAAKHCIMQANAEYHQSILAKQQKK 282

Query: 326 -GVAIGVLRYALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDE 385
            G  I  L++A   +K    R + +  V     D +   L   +++N+ ++H ++P   +
Sbjct: 283 FGEEIARLQHAAELIKTVASRYDEYVNVKDFS-DKINRALAAAKKDNDFIYHDRVPDLKD 342

Query: 386 LPTPKGTQIVKSIP 394
           L       +VKS P
Sbjct: 343 LDPIGKATLVKSTP 342

BLAST of Clc05G25310 vs. ExPASy Swiss-Prot
Match: Q9WU78 (Programmed cell death 6-interacting protein OS=Mus musculus OX=10090 GN=Pdcd6ip PE=1 SV=3)

HSP 1 Score: 51.2 bits (121), Expect = 3.2e-05
Identity = 65/314 (20.70%), Postives = 126/314 (40.13%), Query Frame = 0

Query: 86  AREMSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSS 145
           A E+S    S   + L K +  L  L  +  Q+  I+      +    L   W  A    
Sbjct: 43  AEELSKLRRSALGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKG 102

Query: 146 SLFHLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYL 205
           SLF   G     + +L +E    L    A+    AS++ +E  + +    + AA  YQ+ 
Sbjct: 103 SLF--GGSVKLALASLGYEKSCVLFNCAAL----ASQIAAEQNLDNDEGLKTAAKQYQFA 162

Query: 206 AKEIL---PAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKL 265
           +   L     +   L  E   ++ P T   +SL+ LA+AQ V  ++A       +++AK 
Sbjct: 163 SGAFLHIKDTVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAK- 222

Query: 266 HYGIVELLNESANHLQIISGEC---NDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQ 325
                 L N++A++      +C   + +       L+A + + +  +  + +       +
Sbjct: 223 ------LANQAADYFGDAFKQCQYKDTLPKEVFPTLAAKQCIMQANAEYHQSILAKQQKK 282

Query: 326 AGVAIGVLRYALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDE 385
            G  I  L++A   +K    R + +  V     D +   L   +++N+ ++H ++P   +
Sbjct: 283 FGEEIARLQHAAELIKNVASRYDEYVNVKDFS-DKINRALTAAKKDNDFIYHDRVPDLKD 342

Query: 386 LPTPKGTQIVKSIP 394
           L       +VK  P
Sbjct: 343 LDPIGKATLVKPTP 342

BLAST of Clc05G25310 vs. ExPASy Swiss-Prot
Match: Q9QZA2 (Programmed cell death 6-interacting protein OS=Rattus norvegicus OX=10116 GN=Pdcd6ip PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 3.2e-05
Identity = 68/319 (21.32%), Postives = 127/319 (39.81%), Query Frame = 0

Query: 86  AREMSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSS 145
           A E+S    S   + L K +  L  L  +  Q+  I+      +    L   W  A    
Sbjct: 43  AEELSKLRRSALGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKG 102

Query: 146 SLFHLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYL 205
           SLF   G     + +L +E    L    A+    AS++ +E  + +    + AA  YQ+ 
Sbjct: 103 SLF--GGSVKLALASLGYEKSCVLFNCAAL----ASQIAAEQNLDNDEGLKTAAKQYQFA 162

Query: 206 AKEIL---PAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKL 265
           +   L     +   L  E   ++ P T   +SL+ LA+AQ V  ++A       +++AK 
Sbjct: 163 SGAFLHIKDTVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAK- 222

Query: 266 HYGIVELLNESANHLQIISGEC--NDMSSNFLEFLSAFRALHE----LKSRKNLAKELMS 325
                 L N++A++      +C   D    +  F   F  L      +++     + +++
Sbjct: 223 ------LANQAADYFGDAFKQCQYKDALPKYFYFQEVFPTLAAKQCIMQANAEYHQSILA 282

Query: 326 GGQA--GVAIGVLRYALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKI 385
             Q   G  I  L++A   +K    R + +  V     D +   L   +++N+ ++H ++
Sbjct: 283 KQQKKFGEEIARLQHAAELIKNVASRYDEYVNVKDFS-DKINRALAAAKKDNDFIYHDRV 342

Query: 386 PSRDELPTPKGTQIVKSIP 394
           P   +L       +VK  P
Sbjct: 343 PDLKDLDPIGKATLVKPTP 347

BLAST of Clc05G25310 vs. ExPASy TrEMBL
Match: A0A1S3BFB8 (uncharacterized protein LOC103489257 OS=Cucumis melo OX=3656 GN=LOC103489257 PE=4 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.0e-196
Identity = 354/379 (93.40%), Postives = 365/379 (96.31%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDP+KLKTKMCVFEDKF+ARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE
Sbjct: 1   MLLQFSDPSKLKTKMCVFEDKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKLQQYLPLLENF+SQVDM+DYNLRIVQWTSDLKIRWSSALSSS LF
Sbjct: 61  MSGGLSSHTQQVLHKLQQYLPLLENFISQVDMLDYNLRIVQWTSDLKIRWSSALSSSLLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEVGM LSLYGA LRQRA EVISEDQVQSATFFREAAGVYQYLA+E
Sbjct: 121 HLRGPKFFQIDNLRFEVGMMLSLYGAFLRQRALEVISEDQVQSATFFREAAGVYQYLAEE 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP IQHCLPSERPPEVIPSTSA MSL+CLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE
Sbjct: 181 ILPTIQHCLPSERPPEVIPSTSAAMSLICLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNESANHL I SGE  DMSSNFLEFLSAFRALHEL SRKNLAKELMSGGQ+GVAIGVLR
Sbjct: 241 LLNESANHLHISSGESKDMSSNFLEFLSAFRALHELMSRKNLAKELMSGGQSGVAIGVLR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           YALTDVKKEMPRENSWKLVFG EIDIV ETLRKFERENEIVWHKKIPSRDELPTP GTQI
Sbjct: 301 YALTDVKKEMPRENSWKLVFGIEIDIVTETLRKFERENEIVWHKKIPSRDELPTPVGTQI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           VKSIPY+P+KWER+LAFKI
Sbjct: 361 VKSIPYNPKKWERDLAFKI 379

BLAST of Clc05G25310 vs. ExPASy TrEMBL
Match: A0A0A0KR44 (BRO1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608410 PE=4 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.9e-195
Identity = 354/379 (93.40%), Postives = 364/379 (96.04%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDPAKLKTKMCVFEDKF+ARDSGTLEQLKE+TSRRRVIEESINETSSITDAIARE
Sbjct: 1   MLLQFSDPAKLKTKMCVFEDKFIARDSGTLEQLKEITSRRRVIEESINETSSITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF
Sbjct: 61  MSGGLSSHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEVGMTLSLYGA LRQRA E+ISEDQVQSATFFREA+GVYQYLA+E
Sbjct: 121 HLRGPKFFQIDNLRFEVGMTLSLYGAFLRQRALEIISEDQVQSATFFREASGVYQYLAEE 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP IQHCLPSERPPEVIPSTSA MSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE
Sbjct: 181 ILPTIQHCLPSERPPEVIPSTSAAMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNESAN+L I  GEC DMSSNFLEFLSAFRALHEL SRKNLAKELMS GQ GVAIGVLR
Sbjct: 241 LLNESANYLHISFGECKDMSSNFLEFLSAFRALHELMSRKNLAKELMSSGQVGVAIGVLR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           YALTDVKKEMPRENSWKLVFG EIDIVAETLRKFERENEIVWHKKI  RDELPTP GT+I
Sbjct: 301 YALTDVKKEMPRENSWKLVFGIEIDIVAETLRKFERENEIVWHKKIALRDELPTPIGTRI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           VKSIPY+P+KWERELAFKI
Sbjct: 361 VKSIPYNPKKWERELAFKI 379

BLAST of Clc05G25310 vs. ExPASy TrEMBL
Match: A0A6J1FZA5 (uncharacterized protein LOC111449253 OS=Cucurbita moschata OX=3662 GN=LOC111449253 PE=4 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 5.9e-188
Identity = 341/379 (89.97%), Postives = 362/379 (95.51%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDPAKLKTKMCVFEDKF+ARDS TLEQLKEMTSRRRVIEESINETSSITDAIARE
Sbjct: 1   MLLQFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRRVIEESINETSSITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKL QYLPLLENFVSQVD++ YNL+IVQWTSDLKIRWSSALSSSSLF
Sbjct: 61  MSGGLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEV MTL LYGAVLRQRA EVISEDQVQSATFFREAAGVYQYLAK+
Sbjct: 121 HLRGPKFFQIDNLRFEVAMTLFLYGAVLRQRALEVISEDQVQSATFFREAAGVYQYLAKD 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP+IQHC+PSERPPEVIPSTS  MSLVCLAEAQAVT+MRAEEKG+I SLLAKLHYGIV+
Sbjct: 181 ILPSIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVD 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNESANHL+I+SGE    SS+FLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIG+LR
Sbjct: 241 LLNESANHLRILSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGILR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           +ALTDVKK+MPRENSW+LVFG EID VAETLRKFERENEIVWHKKIPSRDELPTPKG+QI
Sbjct: 301 HALTDVKKDMPRENSWRLVFGTEIDSVAETLRKFERENEIVWHKKIPSRDELPTPKGSQI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           V SIPY P+KWERELAFK+
Sbjct: 361 VSSIPYCPKKWERELAFKM 375

BLAST of Clc05G25310 vs. ExPASy TrEMBL
Match: A0A6J1HUA9 (uncharacterized protein LOC111467531 OS=Cucurbita maxima OX=3661 GN=LOC111467531 PE=4 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.7e-185
Identity = 337/379 (88.92%), Postives = 360/379 (94.99%), Query Frame = 0

Query: 29  MLLQFSDPAKLKTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIARE 88
           MLLQFSDPAKLKTKMCVFEDKF+ARDS TLEQLKEMTSRR+VIEESINETS ITDAIARE
Sbjct: 1   MLLQFSDPAKLKTKMCVFEDKFIARDSSTLEQLKEMTSRRKVIEESINETSFITDAIARE 60

Query: 89  MSGGLASHTQQVLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLF 148
           MSGGL+SHTQQVLHKL QYLPLLENFVSQVD++ YNL+IVQWTSDLKIRWSSALSSSSLF
Sbjct: 61  MSGGLSSHTQQVLHKLLQYLPLLENFVSQVDLVGYNLKIVQWTSDLKIRWSSALSSSSLF 120

Query: 149 HLRGPKFFQIDNLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKE 208
           HLRGPKFFQIDNLRFEV MTL LYGAVLR+RA EVISED+VQSATFFREAAGVYQYLAK+
Sbjct: 121 HLRGPKFFQIDNLRFEVAMTLFLYGAVLRKRALEVISEDKVQSATFFREAAGVYQYLAKD 180

Query: 209 ILPAIQHCLPSERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVE 268
           ILP+IQHC+PSERPPEVIPSTS  MSLVCLAEAQAVT+MRAEEKG+I SLLAKLHYGIV+
Sbjct: 181 ILPSIQHCVPSERPPEVIPSTSVAMSLVCLAEAQAVTTMRAEEKGSISSLLAKLHYGIVD 240

Query: 269 LLNESANHLQIISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLR 328
           LLNESANHL+I SGE    SS+FLEFLSAFRALHELKSRKNLAKELMSGGQ G+AIG+LR
Sbjct: 241 LLNESANHLRIFSGE----SSSFLEFLSAFRALHELKSRKNLAKELMSGGQVGLAIGILR 300

Query: 329 YALTDVKKEMPRENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQI 388
           +ALTDVKKEMPRENSWKLVFG EIDIVAETLRKFERENEIVWHKKIPSRDELPT KG++I
Sbjct: 301 HALTDVKKEMPRENSWKLVFGTEIDIVAETLRKFERENEIVWHKKIPSRDELPTLKGSRI 360

Query: 389 VKSIPYHPQKWERELAFKI 408
           VKSIPY P+KWERELAFK+
Sbjct: 361 VKSIPYCPKKWERELAFKM 375

BLAST of Clc05G25310 vs. ExPASy TrEMBL
Match: A0A5A7SYL1 (BRO1 domain-containing protein BROX OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00750 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 5.2e-176
Identity = 321/341 (94.13%), Postives = 328/341 (96.19%), Query Frame = 0

Query: 43  MCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMSGGLASHTQQVLH 102
           MCVFEDKF+ARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMSGGL+SHTQQVLH
Sbjct: 1   MCVFEDKFIARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMSGGLSSHTQQVLH 60

Query: 103 KLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLFHLRGPKFFQIDNLR 162
           KLQQYLPLLENF+SQVDM+DYNLRIVQWTSDLKIRWSSALSSS LFHLRGPKFFQIDNLR
Sbjct: 61  KLQQYLPLLENFISQVDMLDYNLRIVQWTSDLKIRWSSALSSSLLFHLRGPKFFQIDNLR 120

Query: 163 FEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKEILPAIQHCLPSERP 222
           FEVGM LSLYGA LRQRA EVISEDQVQSATFFREAAGVYQYLA+EILP IQHCLPSERP
Sbjct: 121 FEVGMMLSLYGAFLRQRALEVISEDQVQSATFFREAAGVYQYLAEEILPTIQHCLPSERP 180

Query: 223 PEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLNESANHLQIISG 282
           PEVIPSTSA MSL+CLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLNESANHL I SG
Sbjct: 181 PEVIPSTSAAMSLICLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLNESANHLHISSG 240

Query: 283 ECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLRYALTDVKKEMPREN 342
           E  DMSSNFLEFLSAFRALHEL SRKNLAKELMSGGQ+GVAIGVLRYALTDVKKEMPREN
Sbjct: 241 ESKDMSSNFLEFLSAFRALHELMSRKNLAKELMSGGQSGVAIGVLRYALTDVKKEMPREN 300

Query: 343 SWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTP 384
           SWKLVFG EIDIV ETLRKFERENEIVWHKKIPSRDELPTP
Sbjct: 301 SWKLVFGIEIDIVTETLRKFERENEIVWHKKIPSRDELPTP 341

BLAST of Clc05G25310 vs. TAIR 10
Match: AT1G13310.1 (Endosomal targeting BRO1-like domain-containing protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.9e-83
Identity = 170/369 (46.07%), Postives = 240/369 (65.04%), Query Frame = 0

Query: 40  KTKMCVFEDKFLARDSGTLEQLKEMTSRRRVIEESINETSSITDAIAREMSGGLASHTQQ 99
           KTK  ++E    A    TL ++KE+TS+R++IEES+N+TS + D+  REM+ GL S  +Q
Sbjct: 12  KTKEILYEKTLQAAGPITLGEVKELTSKRKIIEESVNKTSKVIDSTTREMTRGLTSACEQ 71

Query: 100 VLHKLQQYLPLLENFVSQVDMIDYNLRIVQWTSDLKIRWSSALSSSSLFHLRGPKFFQID 159
            LHKL +YLPLL N V   D I       +  S+LKIRWSS L S +L     PKFFQ+D
Sbjct: 72  DLHKLGEYLPLLFNLVHYTDKI-------KRVSNLKIRWSSGLISQTLIQRTCPKFFQVD 131

Query: 160 NLRFEVGMTLSLYGAVLRQRASEVISEDQVQSATFFREAAGVYQYLAKEILPAIQHCLPS 219
           N+ FE GM L +Y   LR+RA E++S D  ++   +REA+GV+ +L+ EILP +Q CLP 
Sbjct: 132 NIMFEFGMVLFIYAVKLRERAMELVSTDSKKAVAVYREASGVFHHLSHEILPLLQTCLPP 191

Query: 220 ERPPEVIPSTSAVMSLVCLAEAQAVTSMRAEEKGTIPSLLAKLHYGIVELLNESANHLQI 279
            + PE+ P   + +SL+CLAE QAVT+ +AEE G   SLL+KLH+GI + L+E+   L  
Sbjct: 192 GKLPELTPPLCSALSLLCLAEGQAVTTEKAEESGKSASLLSKLHFGIFQFLSEAYALLSS 251

Query: 280 -ISGECNDMSSNFLEFLSAFRALHELKSRKNLAKELMSGGQAGVAIGVLRYALTDVKKEM 339
            ++GE  D+S+ FLE+++   ALHELKS+K LA+ L S  + G A+GVLR AL   KK  
Sbjct: 252 RLTGEYKDLSTRFLEYVTTMGALHELKSQKYLAELLESEDRVGDAVGVLRRALAAAKKST 311

Query: 340 P-RENSWKLVFGAEIDIVAETLRKFERENEIVWHKKIPSRDELPTPKGTQIVKSIPYHPQ 399
           P +++ W  +F  E + VA+ + K+E+ N+ +  +KIP   E+P PKG +IV  IPY P 
Sbjct: 312 PSKDDKWIAIFKKEREDVAKNMAKYEKLNDSMMLQKIPIDREIPFPKGEKIVNLIPYTPT 371

Query: 400 KWERELAFK 407
           +  REL FK
Sbjct: 372 RVVRELRFK 373