Clc05G22550 (gene) Watermelon (cordophanus) v2

BLAST of Clc05G22550 vs. NCBI nr
Match: XP_038899610.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 548.9 bits (1413), Expect = 2.7e-152
Identity = 272/302 (90.07%), Postives = 283/302 (93.71%), Query Frame = 0

Query: 1   MLYFCLLFLTAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQ 60
           MLY CLLFLTAAQIP TSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQ
Sbjct: 1   MLYLCLLFLTAAQIPVTSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQ 60

Query: 61  CSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLVP 120
           CSPTPSILRLA+DLTWVEAREPLHADIDFLKTNGIGPGMPFA+ ILMDKPGGR V+GLVP
Sbjct: 61  CSPTPSILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFAHTILMDKPGGRTVIGLVP 120

Query: 121 CAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGRL 180
           CAIGGTSIKEWQQGSNLYNHLLSRAEASVL GGKIKALLWYQGE DTE+AEDSELYG RL
Sbjct: 121 CAIGGTSIKEWQQGSNLYNHLLSRAEASVLSGGKIKALLWYQGESDTENAEDSELYGSRL 180

Query: 181 KKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEPDG 240
           KKFFTDIRSDLKIPLLPIIQVGIA+GEGPYKEGVRRGQFG++LVNVMT+DA+GL LEPDG
Sbjct: 181 KKFFTDIRSDLKIPLLPIIQVGIATGEGPYKEGVRRGQFGIDLVNVMTVDAMGLSLEPDG 240

Query: 241 LHLNTPSQVRLGGLLADAYRRFPSHPLATPLTNAA---PISTFFLSSCRIFTFLFLFLHL 300
           LHL TPSQV+LGGLLADAYRRFPSHPLATPLTNAA    ISTFFLS  RI T + LFL L
Sbjct: 241 LHLTTPSQVQLGGLLADAYRRFPSHPLATPLTNAAHIPMISTFFLSISRILTVVLLFLRL 300

BLAST of Clc05G22550 vs. NCBI nr
Match: XP_008452605.1 (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo] >KAA0064418.1 putative carbohydrate esterase [Cucumis melo var. makuwa] >TYK20169.1 putative carbohydrate esterase [Cucumis melo var. makuwa])

HSP 1 Score: 526.9 bits (1356), Expect = 1.1e-145
Identity = 263/301 (87.38%), Postives = 275/301 (91.36%), Query Frame = 0

Query: 1   MLYFCLLFL--TAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVP 60
           MLY CLLFL  TAAQIP+ SQQPSPPT IFLLAGQSNMAGRGGVTN T+TH+PTWDGVVP
Sbjct: 1   MLYLCLLFLTTTAAQIPSASQQPSPPTDIFLLAGQSNMAGRGGVTNGTLTHQPTWDGVVP 60

Query: 61  PQCSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGL 120
           PQCSPTP ILR A+DLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKPGGR V+GL
Sbjct: 61  PQCSPTPYILRFAADLTWVEAREPLHADIDFLKTNGIGPGMPFANAILMDKPGGRTVIGL 120

Query: 121 VPCAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGG 180
           VPCAIGGTSIKEWQ+GSNLYNHLLSRAEAS+L GGKIKALLWYQGE DTE+AEDSELYGG
Sbjct: 121 VPCAIGGTSIKEWQKGSNLYNHLLSRAEASILRGGKIKALLWYQGESDTENAEDSELYGG 180

Query: 181 RLKKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEP 240
           RLKKF TDIRSDLKIPLLPIIQVGIASGEG YKEGVRRGQFG++LVNVM +DALGLPLEP
Sbjct: 181 RLKKFLTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEP 240

Query: 241 DGLHLNTPSQVRLGGLLADAYRRFPSHPLATPLTNAAP----ISTFFLSSCRIFTFLFLF 296
           DGLHL TPSQVRLGGLLA AYRRFPSHPLATPLTNAAP    ISTF LS   IFTFLF F
Sbjct: 241 DGLHLTTPSQVRLGGLLAHAYRRFPSHPLATPLTNAAPISTIISTFLLSIWWIFTFLFPF 300

BLAST of Clc05G22550 vs. NCBI nr
Match: XP_004141392.1 (probable carbohydrate esterase At4g34215 [Cucumis sativus] >KGN55236.1 hypothetical protein Csa_012338 [Cucumis sativus])

HSP 1 Score: 519.2 bits (1336), Expect = 2.3e-143
Identity = 259/301 (86.05%), Postives = 272/301 (90.37%), Query Frame = 0

Query: 1   MLYFCLLFL-TAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPP 60
           MLY CLLFL T AQIP+TSQQPSPPT IFLLAGQSNMAGRGGVTNST+TH PTWDGVVPP
Sbjct: 1   MLYLCLLFLTTVAQIPSTSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHPTWDGVVPP 60

Query: 61  QCSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLV 120
           QCSPTP ILRLA+DLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKPGGR V+GLV
Sbjct: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGR 180
           PCA+GGTSIKEWQ+GSNLYNHLLSRA+ASVL GGKIKALLWYQGE DTE+AEDSELYGGR
Sbjct: 121 PCAMGGTSIKEWQKGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEPD 240
           LKKFFT IRSDLKIPLLPIIQVGIASGEG YKEGVRRGQFG++LVNVM +DALGLPLEPD
Sbjct: 181 LKKFFTGIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEPD 240

Query: 241 GLHLNTPSQVRLGGLLADAYRRFPSHPLATPLTNAAPISTFFLSSCRIFTFLFLFLHLIL 300
           GLHL T SQVRLGGLLADAYRRFPSHPLATPLTNAAPIS       RI T  F F+  + 
Sbjct: 241 GLHLTTTSQVRLGGLLADAYRRFPSHPLATPLTNAAPIS-------RISTIFFPFVGFLR 294

BLAST of Clc05G22550 vs. NCBI nr
Match: XP_022982273.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 496.1 bits (1276), Expect = 2.1e-136
Identity = 245/303 (80.86%), Postives = 269/303 (88.78%), Query Frame = 0

Query: 1   MLYFCLLFLTAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQ 60
           MLYF LLFL AA +P TSQQP PPTAIFLLAGQSNMAGRGGV+N+T+THRPTWDGVVPPQ
Sbjct: 1   MLYFSLLFLMAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVSNTTLTHRPTWDGVVPPQ 60

Query: 61  CSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLVP 120
           CSP PSILRLASDLTWVEAREPLHADIDFLK NGIGPGMPFAN ILMDKP GR V+GLVP
Sbjct: 61  CSPNPSILRLASDLTWVEAREPLHADIDFLKINGIGPGMPFANTILMDKPAGRTVIGLVP 120

Query: 121 CAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGRL 180
           CA+GGTSI++WQ+GSNLYNHLL+RAEAS+LGGG IKALLWYQGE DT +AEDSELYGGRL
Sbjct: 121 CAMGGTSIQQWQKGSNLYNHLLTRAEASILGGGTIKALLWYQGESDTVNAEDSELYGGRL 180

Query: 181 KKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTID--ALGLPLEP 240
           KKFFTDIRSDL IP LPIIQVGIASGEGPYKEGVRRGQFG+E++NVMT+D  ALGL  EP
Sbjct: 181 KKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQFGIEVMNVMTVDAYALGLSFEP 240

Query: 241 DGLHLNTPSQVRLGGLLADAYRRFPSHPL-ATPLTNAAPISTFFLSSCRIF-TFLFLFLH 300
           DGLHLNTPSQV+LGG+LADAYRRFP HPL A+PL NAA  ++ +     +F T  F+FLH
Sbjct: 241 DGLHLNTPSQVKLGGVLADAYRRFPPHPLAASPLRNAASTASVYSFCISMFRTMTFVFLH 300

BLAST of Clc05G22550 vs. NCBI nr
Match: KAG6608446.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 487.3 bits (1253), Expect = 9.8e-134
Identity = 244/306 (79.74%), Postives = 265/306 (86.60%), Query Frame = 0

Query: 1   MLYFCLLFLTAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQ 60
           MLYF LLFL A  +P TSQQP PPTAIFLLAGQSNMAGRGGVTNST+THRPTWD VVPPQ
Sbjct: 1   MLYFSLLFLMAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQ 60

Query: 61  CSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLVP 120
           CSP PSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKP GR V+GLVP
Sbjct: 61  CSPNPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVP 120

Query: 121 CAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGRL 180
           CA+GGTSI++WQ+GSNLY HLL+RAEAS+L GG IKALLWYQGE DT +AEDSELYGGRL
Sbjct: 121 CAMGGTSIQQWQKGSNLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRL 180

Query: 181 KKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTID--ALGLPLEP 240
           KKFFTDIRSDL IP LPIIQVGIASGEGPYKEGVRRGQ G+E++NVMT+D  ALGL  EP
Sbjct: 181 KKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEP 240

Query: 241 DGLHLNTPSQVRLGGLLADAYRRFPSHPL-ATPL----TNAAPISTFFLSSCRIFTFLFL 300
           DGLHLNTPSQV+LGG+LADAYRRFP HPL A+PL     + A + +FF+S  R  T  FL
Sbjct: 241 DGLHLNTPSQVKLGGILADAYRRFPPHPLAASPLLRSAASTASVFSFFISMFR--TMTFL 300

BLAST of Clc05G22550 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 267.3 bits (682), Expect = 2.1e-70
Identity = 130/241 (53.94%), Postives = 164/241 (68.05%), Query Frame = 0

Query: 19  QQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQCSPTPSILRLASDLTWVE 78
           Q P PP  IF+L+GQSNMAGRGGV      +R  WD ++PP+C+P  SILRL++DL W E
Sbjct: 16  QSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEE 75

Query: 79  AREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLVPCAIGGTSIKEWQQGSNLY 138
           A EPLH DID  K  G+GPGM FAN +         V+GLVPCA GGT+IKEW++GS+LY
Sbjct: 76  AHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLY 135

Query: 139 NHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGRLKKFFTDIRSDLKIPLLPI 198
             ++ R E S   GG+IKA+LWYQGE D     D+E YG  + +   ++R DL +P LPI
Sbjct: 136 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 195

Query: 199 IQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEPDGLHLNTPSQVRLGGLLADA 258
           IQV IASG G Y + VR  Q G++L NV+ +DA GLPL+ D LHL T +QV+LG  LA A
Sbjct: 196 IQVAIASG-GGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQA 255

Query: 259 Y 260
           Y
Sbjct: 256 Y 255

BLAST of Clc05G22550 vs. ExPASy TrEMBL
Match: A0A5A7VA07 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003010 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.4e-146
Identity = 263/301 (87.38%), Postives = 275/301 (91.36%), Query Frame = 0

Query: 1   MLYFCLLFL--TAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVP 60
           MLY CLLFL  TAAQIP+ SQQPSPPT IFLLAGQSNMAGRGGVTN T+TH+PTWDGVVP
Sbjct: 1   MLYLCLLFLTTTAAQIPSASQQPSPPTDIFLLAGQSNMAGRGGVTNGTLTHQPTWDGVVP 60

Query: 61  PQCSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGL 120
           PQCSPTP ILR A+DLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKPGGR V+GL
Sbjct: 61  PQCSPTPYILRFAADLTWVEAREPLHADIDFLKTNGIGPGMPFANAILMDKPGGRTVIGL 120

Query: 121 VPCAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGG 180
           VPCAIGGTSIKEWQ+GSNLYNHLLSRAEAS+L GGKIKALLWYQGE DTE+AEDSELYGG
Sbjct: 121 VPCAIGGTSIKEWQKGSNLYNHLLSRAEASILRGGKIKALLWYQGESDTENAEDSELYGG 180

Query: 181 RLKKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEP 240
           RLKKF TDIRSDLKIPLLPIIQVGIASGEG YKEGVRRGQFG++LVNVM +DALGLPLEP
Sbjct: 181 RLKKFLTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEP 240

Query: 241 DGLHLNTPSQVRLGGLLADAYRRFPSHPLATPLTNAAP----ISTFFLSSCRIFTFLFLF 296
           DGLHL TPSQVRLGGLLA AYRRFPSHPLATPLTNAAP    ISTF LS   IFTFLF F
Sbjct: 241 DGLHLTTPSQVRLGGLLAHAYRRFPSHPLATPLTNAAPISTIISTFLLSIWWIFTFLFPF 300

BLAST of Clc05G22550 vs. ExPASy TrEMBL
Match: A0A1S3BVE3 (probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103493577 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.4e-146
Identity = 263/301 (87.38%), Postives = 275/301 (91.36%), Query Frame = 0

Query: 1   MLYFCLLFL--TAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVP 60
           MLY CLLFL  TAAQIP+ SQQPSPPT IFLLAGQSNMAGRGGVTN T+TH+PTWDGVVP
Sbjct: 1   MLYLCLLFLTTTAAQIPSASQQPSPPTDIFLLAGQSNMAGRGGVTNGTLTHQPTWDGVVP 60

Query: 61  PQCSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGL 120
           PQCSPTP ILR A+DLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKPGGR V+GL
Sbjct: 61  PQCSPTPYILRFAADLTWVEAREPLHADIDFLKTNGIGPGMPFANAILMDKPGGRTVIGL 120

Query: 121 VPCAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGG 180
           VPCAIGGTSIKEWQ+GSNLYNHLLSRAEAS+L GGKIKALLWYQGE DTE+AEDSELYGG
Sbjct: 121 VPCAIGGTSIKEWQKGSNLYNHLLSRAEASILRGGKIKALLWYQGESDTENAEDSELYGG 180

Query: 181 RLKKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEP 240
           RLKKF TDIRSDLKIPLLPIIQVGIASGEG YKEGVRRGQFG++LVNVM +DALGLPLEP
Sbjct: 181 RLKKFLTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEP 240

Query: 241 DGLHLNTPSQVRLGGLLADAYRRFPSHPLATPLTNAAP----ISTFFLSSCRIFTFLFLF 296
           DGLHL TPSQVRLGGLLA AYRRFPSHPLATPLTNAAP    ISTF LS   IFTFLF F
Sbjct: 241 DGLHLTTPSQVRLGGLLAHAYRRFPSHPLATPLTNAAPISTIISTFLLSIWWIFTFLFPF 300

BLAST of Clc05G22550 vs. ExPASy TrEMBL
Match: A0A0A0L4Z7 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G641680 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.1e-143
Identity = 259/301 (86.05%), Postives = 272/301 (90.37%), Query Frame = 0

Query: 1   MLYFCLLFL-TAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPP 60
           MLY CLLFL T AQIP+TSQQPSPPT IFLLAGQSNMAGRGGVTNST+TH PTWDGVVPP
Sbjct: 1   MLYLCLLFLTTVAQIPSTSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHPTWDGVVPP 60

Query: 61  QCSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLV 120
           QCSPTP ILRLA+DLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKPGGR V+GLV
Sbjct: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGR 180
           PCA+GGTSIKEWQ+GSNLYNHLLSRA+ASVL GGKIKALLWYQGE DTE+AEDSELYGGR
Sbjct: 121 PCAMGGTSIKEWQKGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEPD 240
           LKKFFT IRSDLKIPLLPIIQVGIASGEG YKEGVRRGQFG++LVNVM +DALGLPLEPD
Sbjct: 181 LKKFFTGIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEPD 240

Query: 241 GLHLNTPSQVRLGGLLADAYRRFPSHPLATPLTNAAPISTFFLSSCRIFTFLFLFLHLIL 300
           GLHL T SQVRLGGLLADAYRRFPSHPLATPLTNAAPIS       RI T  F F+  + 
Sbjct: 241 GLHLTTTSQVRLGGLLADAYRRFPSHPLATPLTNAAPIS-------RISTIFFPFVGFLR 294

BLAST of Clc05G22550 vs. ExPASy TrEMBL
Match: A0A6J1J4F1 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111481152 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.0e-136
Identity = 245/303 (80.86%), Postives = 269/303 (88.78%), Query Frame = 0

Query: 1   MLYFCLLFLTAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQ 60
           MLYF LLFL AA +P TSQQP PPTAIFLLAGQSNMAGRGGV+N+T+THRPTWDGVVPPQ
Sbjct: 1   MLYFSLLFLMAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVSNTTLTHRPTWDGVVPPQ 60

Query: 61  CSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLVP 120
           CSP PSILRLASDLTWVEAREPLHADIDFLK NGIGPGMPFAN ILMDKP GR V+GLVP
Sbjct: 61  CSPNPSILRLASDLTWVEAREPLHADIDFLKINGIGPGMPFANTILMDKPAGRTVIGLVP 120

Query: 121 CAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGRL 180
           CA+GGTSI++WQ+GSNLYNHLL+RAEAS+LGGG IKALLWYQGE DT +AEDSELYGGRL
Sbjct: 121 CAMGGTSIQQWQKGSNLYNHLLTRAEASILGGGTIKALLWYQGESDTVNAEDSELYGGRL 180

Query: 181 KKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTID--ALGLPLEP 240
           KKFFTDIRSDL IP LPIIQVGIASGEGPYKEGVRRGQFG+E++NVMT+D  ALGL  EP
Sbjct: 181 KKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQFGIEVMNVMTVDAYALGLSFEP 240

Query: 241 DGLHLNTPSQVRLGGLLADAYRRFPSHPL-ATPLTNAAPISTFFLSSCRIF-TFLFLFLH 300
           DGLHLNTPSQV+LGG+LADAYRRFP HPL A+PL NAA  ++ +     +F T  F+FLH
Sbjct: 241 DGLHLNTPSQVKLGGVLADAYRRFPPHPLAASPLRNAASTASVYSFCISMFRTMTFVFLH 300

BLAST of Clc05G22550 vs. ExPASy TrEMBL
Match: A0A6J1FS50 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111446742 PE=4 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.4e-133
Identity = 245/306 (80.07%), Postives = 267/306 (87.25%), Query Frame = 0

Query: 1   MLYFCLLF-LTAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPP 60
           MLYF LLF L AA +P TSQQP PPTAIFLLAGQSNMAGRGGVTNST+THRPTWDGVVPP
Sbjct: 28  MLYFSLLFLLMAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDGVVPP 87

Query: 61  QCSPTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLV 120
           QCSP PSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKP GR V+GLV
Sbjct: 88  QCSPNPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLV 147

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGR 180
           PCA+GGTSI++WQ+GSNLY HLL+RAEAS+L GG IKALLWYQGE DT +AEDSELYGGR
Sbjct: 148 PCAMGGTSIQQWQKGSNLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGR 207

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTID--ALGLPLE 240
           L KFFTDIRSDL IP LPIIQVGIASGEGPYKEGVRRGQ G+E++NVMT+D  ALGL  E
Sbjct: 208 LNKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFE 267

Query: 241 PDGLHLNTPSQVRLGGLLADAYRRFPSHPL-ATPLTNA---APISTFFLSSCRIFTFLFL 300
           PDGLHLNTPSQV+LGG+LADAYRRFP HPL A+PL +A   A + +FF+S  R  T  F+
Sbjct: 268 PDGLHLNTPSQVKLGGVLADAYRRFPPHPLAASPLRSAASTASVHSFFISMFR--TLTFV 327

BLAST of Clc05G22550 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 284.6 bits (727), Expect = 8.9e-77
Identity = 146/295 (49.49%), Postives = 194/295 (65.76%), Query Frame = 0

Query: 3   YFCLLFLTAAQIPATSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQCS 62
           YF ++    +     SQ  +   +IF+LAGQSNMAGRGGV N T T+   WDGV+PP+C 
Sbjct: 7   YFFIIIFLQSPPHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECR 66

Query: 63  PTPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLVPCA 122
             PSILRL S L W EA+EPLH DID  KTNG+GPGMPFAN + +++ G    VGLVPC+
Sbjct: 67  SNPSILRLTSKLEWKEAKEPLHVDIDINKTNGVGPGMPFANRV-VNRFG---QVGLVPCS 126

Query: 123 IGGTSIKEWQQGSNLYNHLLSRAEASVL--GGGKIKALLWYQGERDTESAEDSELYGGRL 182
           IGGT + +WQ+G  LY   + RA+A++   GGG  +A+LWYQGE DT    D+ +Y  RL
Sbjct: 127 IGGTKLSQWQKGEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRL 186

Query: 183 KKFFTDIRSDLKIPLLPIIQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEPDG 242
            KFF+D+R+DL+ P LPIIQV +A+G GPY + VR+ Q   +L NV  +DA GLPLEPDG
Sbjct: 187 VKFFSDLRNDLQHPNLPIIQVALATGAGPYLDAVRKAQLKTDLENVYCVDARGLPLEPDG 246

Query: 243 LHLNTPSQVRLGGLLADAYRRFPSHPLATPLTNAAPISTFFLSSCRIFTFLFLFL 296
           LHL T SQV+LG ++A+++   P+    +   +   +      S  IF  +F FL
Sbjct: 247 LHLTTSSQVQLGHMIAESFLAIPNSARPSLYMHFFSLFCSLYVSLNIFGTIFHFL 297

BLAST of Clc05G22550 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-71
Identity = 130/241 (53.94%), Postives = 164/241 (68.05%), Query Frame = 0

Query: 19  QQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQCSPTPSILRLASDLTWVE 78
           Q P PP  IF+L+GQSNMAGRGGV      +R  WD ++PP+C+P  SILRL++DL W E
Sbjct: 16  QSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEE 75

Query: 79  AREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLVPCAIGGTSIKEWQQGSNLY 138
           A EPLH DID  K  G+GPGM FAN +         V+GLVPCA GGT+IKEW++GS+LY
Sbjct: 76  AHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLY 135

Query: 139 NHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGRLKKFFTDIRSDLKIPLLPI 198
             ++ R E S   GG+IKA+LWYQGE D     D+E YG  + +   ++R DL +P LPI
Sbjct: 136 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 195

Query: 199 IQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEPDGLHLNTPSQVRLGGLLADA 258
           IQV IASG G Y + VR  Q G++L NV+ +DA GLPL+ D LHL T +QV+LG  LA A
Sbjct: 196 IQVAIASG-GGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQA 255

Query: 259 Y 260
           Y
Sbjct: 256 Y 255

BLAST of Clc05G22550 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-71
Identity = 130/241 (53.94%), Postives = 164/241 (68.05%), Query Frame = 0

Query: 19  QQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPPQCSPTPSILRLASDLTWVE 78
           Q P PP  IF+L+GQSNMAGRGGV      +R  WD ++PP+C+P  SILRL++DL W E
Sbjct: 16  QSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEE 75

Query: 79  AREPLHADIDFLKTNGIGPGMPFANNILMDKPGGRRVVGLVPCAIGGTSIKEWQQGSNLY 138
           A EPLH DID  K  G+GPGM FAN +         V+GLVPCA GGT+IKEW++GS+LY
Sbjct: 76  AHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLY 135

Query: 139 NHLLSRAEASVLGGGKIKALLWYQGERDTESAEDSELYGGRLKKFFTDIRSDLKIPLLPI 198
             ++ R E S   GG+IKA+LWYQGE D     D+E YG  + +   ++R DL +P LPI
Sbjct: 136 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 195

Query: 199 IQVGIASGEGPYKEGVRRGQFGMELVNVMTIDALGLPLEPDGLHLNTPSQVRLGGLLADA 258
           IQV IASG G Y + VR  Q G++L NV+ +DA GLPL+ D LHL T +QV+LG  LA A
Sbjct: 196 IQVAIASG-GGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQA 255

Query: 259 Y 260
           Y
Sbjct: 256 Y 255