Clc05G22280 (gene) Watermelon (cordophanus) v2

Overview
NameClc05G22280
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Descriptionheat stress transcription factor B-4-like
LocationClcChr05: 30584731 .. 30588530 (-)
RNA-Seq ExpressionClc05G22280
SyntenyClc05G22280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTCAGTAGAGAGATAAAAATAAAAATTATAGAAATATATACATATATATATATATATATATTTTTTTTTTAAAAAAAAAACATGTCACGGGTTTGTCTTGAAACCCTCTTATTTCACAGACACATATTCTCTAATTTGTTAATATAAAATCAAAAACTAACTGCACACCACACGACACACTACCTTTCCTCTTTCTTTTTGCTTCTCTTTTATTCCTTCATCTGTTCTCAACACAAACACAAACACAAACACAAAACCTCTGAAATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCTCACAAAGCAATCCCAGCTCCGTTTCTCACTAAAACCTACCAACTGGTTGATGATCCTTCCACTGACCATATTGTCTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTTCCTAACTATTTCAAACACAACAATTTCTCTAGCTTCGTCCGTCAGCTCAACACTTATGTAATTAATTAACCCCTATTTTACATCATCAATCATTTATCTAACTTCACTAATCTCTATCTTTCTTTCTTTTGTTCTTTTTTTTCTAGGGTTTTAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAATGAGTTCTTTAGAAAAGGAGAGAAACATTTGTTATGTGAGATCCATAGACGGAAAACCGCTCAACCTCAAGTTACCGTCAACCAACACCACCAATCTCATTCTCCACTTGGTATTAACAATCCCGGGTTTTACCATTTTCCCGGCAGAGTTAGTATCTCCCCCTCCGATTCCGACGACCAAAACAATTGGTGCGACTCGCCTCCACTCTCTTCCTCTGGTCCCCACAGCGGCGTTCACAACAACAACACCAACTCCGTCACCGCTTTGTCGGAGGACAACGAACGCCTCCGCCGAAGTAACAACATGCTGATGTCCGAATTAGCCCATATGAAAAAACTCTACAACGACATCATTTATTTCGTTCAAAACCATGTGAAGCCTGTCGCCCCGAGTAATTCATATCAATATTCAACCACATCCTTACTTTCCGACGGCTTTCCGGTGGTGCAACACCGGCCACCAAACCACCACCATCATTACCATCATCTGACGAATGGGAAACAAGTTTCGAGTCAGATCCAATTGGTGACGGCAACTACTCCAAATAATAATACTAACAAGAATGCTGCAACGAAAAGCTTCGTGACGATTCTAGAAGAACAACAACAGCAACAACAACAAACGAAAACAAAGCTTTTTGGTGTGGCGATTCAATCCAAGAAACGGTTACACCCAGAGTATGCTTCTAACAACATTGGGAAAGAGAACAACAACAAAGCTAGATTGGTTTTGGAAAAAGACGATTTAGGCCTCAATCTCATGCCTCCTTCCGCTTGTTAGTTCCAATTTTCCTTTCATTTTCATTTACTTTTTTCCTTTTTTACTATTTCTTACAAATCCCAGAGGGGAATCCTTTTTCCAGTGCTGCATTATTATTCATCATAATTGAATTAGAAAATGTGTGCGTGCGTATGGTTGTTTGTGATAGCAGTGTAAGTTTCTATGTATATAACCCTCAGTGACCACCTCTGGAATCTGTTCTTTCAAATTAACATGACTTTTTTTTTTTTTTTTTTTTTGGGATTTTATTCAAAATGCTTGTTTATCATCATATGGGTTTGACCTGGAATATTTTCAGTAGCATTAATGTGGGATAGTGATTAATGAAGGAGTGAGTTATAATGAAAGGAGTACGAAAAAGGGGTTCTTAAATGGAAAGTTTTTTTTTGCTTATCATTTCAGTTCTACACAGAAACAAGGGCAAAATATTTGTATGAGGGAGTGATGAGAAAGACAGTAAGTATTAAATGAATAAGGTGTGTGTGCTGACACTAAGGAGAAGAGAAGAAGAAAAGAAAAAGGACAGTTTGTTTTGAAGACCCCCAAATATTTAATAATTGCAGTCACTATATATATATTGGTATACTTAATTAAATAAATAAATAAATATATATATATATATATATATTATAGTCAGGGCCCAAAATTTGATTTTATATGATTTTATCAGAAAGAAGGCTATCATATCATTTGATTCATGTTGGCCGTATTTTACTAATCAATCTCTCTTTCTCTTCTTTCAATGGAAAGGTTAATTAAACAATGATTCCCCAACATTCATCCTAATCTAATATTCTCTTCTCTTATACCCCAAAATTTTAAAAATAAAAATATTGTTACATTTGAAATTTAAACCGAACCCCAACCCCGGCCCATTAAAAACCTCAGCTTTTTCCTACTTAAAGTATATGAGAGGAATGAGTTTTGAAAACCCTAGGGTTTTGAACTCTCCCCTTCTCTTTCACCTACAAATAGAGTGTTTGTGAGTTGTTGCTTCAGACTAGATAGAGTGATGATTGACTGGTAGATAAACCAACCCACTTTGCCGAAATGCCACTCTCTTTTATGGTTTCACTTTTCTCTGTTTTTTGCAGAAACTCCATTACTGGTAAAAAAAAAAAAAAAAAAAAGAACAAAAAAAGAAGAAGAATTTCCTACTTTTTTTTTTTATGAAACGAAAAGTTTGACCATGGCAGGCCAATGTATAACTCTGTGGCCTCTATTGCTTTCCCTTTTCATAAATATATGTGTTTCATGGGGATGGGGGCATTTAATTTCATGACAGAACAATTCAACTATGAAAAACCAAAAAGAAAAAGAAAAGAAAAAAAAATACAGCTGTGTGAAACGCAGAGTTTTATCTAAAAAGAAAGATCCATTTAAAAGAAAAAAAAAAAAAAATAGTCCTCTAGTTCAAGTGTTTAAAAGTTTGGAATATTGTAGGCCTTTCGATATCTTTGTCGTGCAATGATTTCCTCTCTCACATATAATTTCAAAAGCAACCCAGTTGTGAATATTATAATATGCAAAAAGAAATATATGAAAAAAGACACACCACAAAACCCTTCTATGATACCTTTGTTTACGGTCCTGATCAAGCCGTTGTCTTTTCTCATAGGGGGCCTCGCACGTGATTCGGGTGTGCACGTGAAGCCTAAAACAACGCAGCTGGGTTTGGGAAAGAAAGGATGAAAAAAGGGAAATAAGGAAAGAAAAAAAAAAACTCCATTAAAGATGCTTTAAAGGACTTCATTGTAGGTTTTTGTTTGCTACCCAAACCAGCCGTATTCAACCCATTCTCCCATCGGGGATGATTCTTCCAACCTCTAAAGTAAAAGGAAAATATTAAATTCTTTAAATAATTTTCTTTTAGTAAAGAGAAATGCATGTCCACTTTGTCTGAATCGACTTTATTGACATTTTTCTCCTTTCGTTTTCTT

mRNA sequence

TGTCAGTAGAGAGATAAAAATAAAAATTATAGAAATATATACATATATATATATATATATATTTTTTTTTTAAAAAAAAAACATGTCACGGGTTTGTCTTGAAACCCTCTTATTTCACAGACACATATTCTCTAATTTGTTAATATAAAATCAAAAACTAACTGCACACCACACGACACACTACCTTTCCTCTTTCTTTTTGCTTCTCTTTTATTCCTTCATCTGTTCTCAACACAAACACAAACACAAACACAAAACCTCTGAAATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCTCACAAAGCAATCCCAGCTCCGTTTCTCACTAAAACCTACCAACTGGTTGATGATCCTTCCACTGACCATATTGTCTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTTCCTAACTATTTCAAACACAACAATTTCTCTAGCTTCGTCCGTCAGCTCAACACTTATGGTTTTAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAATGAGTTCTTTAGAAAAGGAGAGAAACATTTGTTATGTGAGATCCATAGACGGAAAACCGCTCAACCTCAAGTTACCGTCAACCAACACCACCAATCTCATTCTCCACTTGGTATTAACAATCCCGGGTTTTACCATTTTCCCGGCAGAGTTAGTATCTCCCCCTCCGATTCCGACGACCAAAACAATTGGTGCGACTCGCCTCCACTCTCTTCCTCTGGTCCCCACAGCGGCGTTCACAACAACAACACCAACTCCGTCACCGCTTTGTCGGAGGACAACGAACGCCTCCGCCGAAGTAACAACATGCTGATGTCCGAATTAGCCCATATGAAAAAACTCTACAACGACATCATTTATTTCGTTCAAAACCATGTGAAGCCTGTCGCCCCGAGTAATTCATATCAATATTCAACCACATCCTTACTTTCCGACGGCTTTCCGGTGGTGCAACACCGGCCACCAAACCACCACCATCATTACCATCATCTGACGAATGGGAAACAAGTTTCGAGTCAGATCCAATTGGTGACGGCAACTACTCCAAATAATAATACTAACAAGAATGCTGCAACGAAAAGCTTCGTGACGATTCTAGAAGAACAACAACAGCAACAACAACAAACGAAAACAAAGCTTTTTGGTGTGGCGATTCAATCCAAGAAACGGTTACACCCAGAGTATGCTTCTAACAACATTGGGAAAGAGAACAACAACAAAGCTAGATTGGTTTTGGAAAAAGACGATTTAGGCCTCAATCTCATGCCTCCTTCCGCTTGGGGCCTCGCACGTGATTCGGGTGTGCACGTGAAGCCTAAAACAACGCAGCTGGGTTTGGGAAAGAAAGGATGAAAAAAGGGAAATAAGGAAAGAAAAAAAAAAACTCCATTAAAGATGCTTTAAAGGACTTCATTGTAGGTTTTTGTTTGCTACCCAAACCAGCCGTATTCAACCCATTCTCCCATCGGGGATGATTCTTCCAACCTCTAAAGTAAAAGGAAAATATTAAATTCTTTAAATAATTTTCTTTTAGTAAAGAGAAATGCATGTCCACTTTGTCTGAATCGACTTTATTGACATTTTTCTCCTTTCGTTTTCTT

Coding sequence (CDS)

ATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCTCACAAAGCAATCCCAGCTCCGTTTCTCACTAAAACCTACCAACTGGTTGATGATCCTTCCACTGACCATATTGTCTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTTCCTAACTATTTCAAACACAACAATTTCTCTAGCTTCGTCCGTCAGCTCAACACTTATGGTTTTAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAATGAGTTCTTTAGAAAAGGAGAGAAACATTTGTTATGTGAGATCCATAGACGGAAAACCGCTCAACCTCAAGTTACCGTCAACCAACACCACCAATCTCATTCTCCACTTGGTATTAACAATCCCGGGTTTTACCATTTTCCCGGCAGAGTTAGTATCTCCCCCTCCGATTCCGACGACCAAAACAATTGGTGCGACTCGCCTCCACTCTCTTCCTCTGGTCCCCACAGCGGCGTTCACAACAACAACACCAACTCCGTCACCGCTTTGTCGGAGGACAACGAACGCCTCCGCCGAAGTAACAACATGCTGATGTCCGAATTAGCCCATATGAAAAAACTCTACAACGACATCATTTATTTCGTTCAAAACCATGTGAAGCCTGTCGCCCCGAGTAATTCATATCAATATTCAACCACATCCTTACTTTCCGACGGCTTTCCGGTGGTGCAACACCGGCCACCAAACCACCACCATCATTACCATCATCTGACGAATGGGAAACAAGTTTCGAGTCAGATCCAATTGGTGACGGCAACTACTCCAAATAATAATACTAACAAGAATGCTGCAACGAAAAGCTTCGTGACGATTCTAGAAGAACAACAACAGCAACAACAACAAACGAAAACAAAGCTTTTTGGTGTGGCGATTCAATCCAAGAAACGGTTACACCCAGAGTATGCTTCTAACAACATTGGGAAAGAGAACAACAACAAAGCTAGATTGGTTTTGGAAAAAGACGATTTAGGCCTCAATCTCATGCCTCCTTCCGCTTGGGGCCTCGCACGTGATTCGGGTGTGCACGTGAAGCCTAAAACAACGCAGCTGGGTTTGGGAAAGAAAGGATGA

Protein sequence

MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNTNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLSDGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEEQQQQQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSAWGLARDSGVHVKPKTTQLGLGKKG
Homology
BLAST of Clc05G22280 vs. NCBI nr
Match: XP_038897232.1 (heat stress transcription factor B-4-like [Benincasa hispida])

HSP 1 Score: 621.7 bits (1602), Expect = 4.2e-174
Identity = 319/366 (87.16%), Postives = 322/366 (87.98%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNT 180
           VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSP LSS  P    HNNN 
Sbjct: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPSLSSPAP----HNNNN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240

Query: 241 DGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNT-----------NKNAATK 300
           D FPVVQ RPPNHHHH++H        SQI LVT TTP NN            N N+ TK
Sbjct: 241 DRFPVVQQRPPNHHHHHYH------QQSQIHLVTGTTPTNNNNNHNNNNNIIINNNSGTK 300

Query: 301 SFVTILEEQQQQQQQTKTKLFGVAIQSKKRLHPEYASNNIGKE-NNNKARLVLEKDDLGL 355
           SFVTILEE     Q TKTKLFGVAIQSKKRLHPEY SNNIGKE NNNKARLVLE DDLGL
Sbjct: 301 SFVTILEE-----QPTKTKLFGVAIQSKKRLHPEYGSNNIGKENNNNKARLVLENDDLGL 351

BLAST of Clc05G22280 vs. NCBI nr
Match: KAG6608467.1 (Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 572.8 bits (1475), Expect = 2.2e-159
Identity = 300/355 (84.51%), Postives = 314/355 (88.45%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNT 180
           VTVNQHHQS+SPLG+ N GFYH+P RVSISPSDSDD  NWCDSPPLSS+  +S  +NN  
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNNNSNNNNNFN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TSLLS
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TSLLS 240

Query: 241 DGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEEQQQ 300
           DGFPV Q R PNHH   +H TN KQVS Q   V A TPNNN   N ++KSFVTI+EE   
Sbjct: 241 DGFPVGQ-RQPNHHLLGYHPTNAKQVSGQTHFV-AGTPNNN---NGSSKSFVTIVEE--- 300

Query: 301 QQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
                KTKLFGV++QSKKR+HPEY SNNI KE NNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 ----PKTKLFGVSLQSKKRVHPEYGSNNIVKE-NNKARLVLEKDDLGLNLMPPSA 340

BLAST of Clc05G22280 vs. NCBI nr
Match: XP_022982267.1 (heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 570.5 bits (1469), Expect = 1.1e-158
Identity = 299/355 (84.23%), Postives = 313/355 (88.17%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNT 180
           VTVNQHHQS+SPLG+ N GFYH+P RVSISPSDSDD  NWCDSPPLSS+  ++ V N   
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNNNNNVFN--- 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TSLLS
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TSLLS 240

Query: 241 DGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEEQQQ 300
           DGFPV Q R PNHH   +H TN KQVS Q   V   TPNNN N N ++KSFVTI+EE   
Sbjct: 241 DGFPVGQ-RQPNHHLLGYHPTNAKQVSGQTHFV-GGTPNNN-NNNGSSKSFVTIVEE--- 300

Query: 301 QQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
                KTKLFGV++QSKKR+HPEY SNNI KE NNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 ----PKTKLFGVSLQSKKRVHPEYGSNNIAKE-NNKARLVLEKDDLGLNLMPPSA 339

BLAST of Clc05G22280 vs. NCBI nr
Match: XP_022940239.1 (heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566.6 bits (1459), Expect = 1.6e-157
Identity = 299/355 (84.23%), Postives = 312/355 (87.89%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNT 180
           VTVNQHHQS+SPLG+ N GFYH+P RVSISPSDSDD  NWCDSPPLSS+      +NN  
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNN----NNNFN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TSLLS
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TSLLS 240

Query: 241 DGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEEQQQ 300
           DGFPV Q R PNHH   +H TN KQVS Q   V A TPNNN   N ++KSFVTI+EE   
Sbjct: 241 DGFPVGQ-RQPNHHLLGYHPTNAKQVSGQTHFV-AGTPNNN---NGSSKSFVTIVEE--- 300

Query: 301 QQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
                KTKLFGV++QSKKR+HPEY SNNI KE NNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 ----PKTKLFGVSLQSKKRVHPEYGSNNIVKE-NNKARLVLEKDDLGLNLMPPSA 336

BLAST of Clc05G22280 vs. NCBI nr
Match: XP_004141262.2 (heat stress transcription factor B-4 [Cucumis sativus] >KAE8649863.1 hypothetical protein Csa_012628 [Cucumis sativus])

HSP 1 Score: 558.5 bits (1438), Expect = 4.3e-155
Identity = 297/358 (82.96%), Postives = 309/358 (86.31%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFP-GRVSISPSDSDDQNN-WCDSPPLSSSGPHSGVHNN 180
           VTVNQHHQ HSPL   NPGFYHFP  R+SISPSDSDDQNN WCDSP           +NN
Sbjct: 121 VTVNQHHQPHSPL---NPGFYHFPTARLSISPSDSDDQNNYWCDSP---------SPNNN 180

Query: 181 NTNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TTS 240
           N NSVTALSEDNERLRRSNNMLMSELAH+KKLYNDIIYFVQNHVKPVAPSNSYQYS TTS
Sbjct: 181 NNNSVTALSEDNERLRRSNNMLMSELAHIKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTS 240

Query: 241 LLSDGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEE 300
           LLSDGFPVV  R PNH+HH+HH  + +QVSSQI+           N    TKSFVTILEE
Sbjct: 241 LLSDGFPVV--RQPNHYHHHHH--HHQQVSSQIR-----------NNTVGTKSFVTILEE 300

Query: 301 QQQQQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
             +QQQQTKTKLFGVAIQSKKRLHPEY ++N    NNNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 --EQQQQTKTKLFGVAIQSKKRLHPEYGNSN-SNNNNNKARLVLEKDDLGLNLMPPSA 328

BLAST of Clc05G22280 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.3e-87
Identity = 195/373 (52.28%), Postives = 236/373 (63.27%), Query Frame = 0

Query: 1   MALMLDNCEG----------VLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA+M++N  G           L+     KA+PAPFLTKTYQLVDDP+TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQ--PQ--VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPL 180
           IHRRKT+Q  PQ       HH +   +  +   F+  P     +P   ++ + WCD  P 
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTP---EEDHYWCDDSP- 180

Query: 181 SSSGPHSGVHNNNTNS-VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPV 240
             S P       +T + VTALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVKPV
Sbjct: 181 -PSRPRVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPV 240

Query: 241 APSNSYQYSTTSLLSDGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKN 300
           APSN+  Y     LS      Q + P    +Y+  T           V AT  N   +  
Sbjct: 241 APSNNSSY-----LSSFLQKQQQQQPPTLDYYNTAT-----------VNATNLNALNSSP 300

Query: 301 AATKSFVTILEE----QQQQQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVL 355
             ++S +T+LE+       Q    KTKLFGV++ S K+    ++      +  +K RLVL
Sbjct: 301 PTSQSSITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSHHFS------DQTSKTRLVL 346

BLAST of Clc05G22280 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.7e-69
Identity = 143/261 (54.79%), Postives = 171/261 (65.52%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLD----------SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA +++ C  +++S++          + K +PAPFLTKTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 121 IHRRKTAQPQVTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSG 180
           IHRRK++QP      H   H    +N       P  +   P      +   + P  ++  
Sbjct: 121 IHRRKSSQPPPPPMPHQPYHHHHHLN-------PFSLPPPPPAYHHHHLIQEEPATTAHC 180

Query: 181 PHSGVHNNNTNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNS 240
             +G      + + ALSEDN +LRR N++L+SELAHMKKLYNDIIYF+QNHV PV     
Sbjct: 181 TVAGDGGEGGDFLAALSEDNRQLRRRNSLLLSELAHMKKLYNDIIYFLQNHVAPVTT--- 240

Query: 241 YQYSTTSLLSDGFPVVQHRPP 252
              +TT+  S      QH  P
Sbjct: 241 ---TTTTPSSTAMAAAQHHLP 248

BLAST of Clc05G22280 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 7.9e-67
Identity = 141/243 (58.02%), Postives = 170/243 (69.96%), Query Frame = 0

Query: 1   MALMLDNCEG-VLLSLD-SH----------KAIPAPFLTKTYQLVDDPSTDHIVSWGEDD 60
           MA +++ C G +++S++ SH           A PAPFL+KTYQLVDDPSTD +VSWGED+
Sbjct: 1   MAFLVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDE 60

Query: 61  TTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLL 120
            TFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL
Sbjct: 61  ATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLL 120

Query: 121 CEIHRRKT---AQPQVTVNQHHQSHSPLGI-NNPGFYHFPGRVSISPSDSDDQNNWCDSP 180
            EIHRRK+   +QPQ         H PL + + P     P   + + +    Q  +C SP
Sbjct: 121 SEIHRRKSSSCSQPQPPPPFPMHQHYPLSLFSPPTTPRSPPVGAAAAAAYHFQEEYCSSP 180

Query: 181 PLSSSGPHSGVHNNNTNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKP 228
              + G          + + ALSEDN +LRR N++L+SELAHM+KLYNDIIYF+QNHV+P
Sbjct: 181 ADYAGG--------GGDLLAALSEDNRQLRRRNSLLLSELAHMRKLYNDIIYFLQNHVEP 235

BLAST of Clc05G22280 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.8e-64
Identity = 171/386 (44.30%), Postives = 206/386 (53.37%), Query Frame = 0

Query: 7   NCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDD-TTFVVWRPPEFARDLLP 66
           +CE    +  + KA+PAPFLTKTYQLVDDP+TDHIVSWG+D  +TFVVWRPPEFARD+LP
Sbjct: 9   DCEAAAAA--AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILP 68

Query: 67  NYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA-------- 126
           NYFKHNNFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRKT+        
Sbjct: 69  NYFKHNNFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTASPS 128

Query: 127 --------------QPQVTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWC-- 186
                          P V   QHH  H+ +G +     H  G     P   +        
Sbjct: 129 PPPFFAPPHFPLFHHPGVAAAQHH--HAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATR 188

Query: 187 -----DSPPLSSSGPHSGVHNNNTNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIY 246
                   P  SS    G     T +V  L E+NERLRRSN  L+ ELAHM+KLYNDIIY
Sbjct: 189 LLALGGPAPSPSSAEAGGAGRAATAAV--LMEENERLRRSNTALLQELAHMRKLYNDIIY 248

Query: 247 FVQNHVKPVAPSNSYQYSTTSLLSDGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTAT 306
           FVQNHV+PVAPS        +    G  +   + P   +  ++       SS + +    
Sbjct: 249 FVQNHVRPVAPS-----PAAAAFLQGLGMQARKKPAAANVLNNSGGSTTSSSSLTIAEEP 308

Query: 307 TPNNNTNKNAATKSFVTILEEQQQQQQQTKTKLFGVAIQ-------SKKRLHPEYASNNI 356
           +P       A  KS           +    TKLFGV +        SK+   PE    + 
Sbjct: 309 SPPPQQQHLAGEKSGGE-AGNSSAARSSAPTKLFGVHLSAAPCGAGSKRASSPE---EHP 368

BLAST of Clc05G22280 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.0e-58
Identity = 156/380 (41.05%), Postives = 203/380 (53.42%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHN 76
           S   +PAPFLTKTYQLVDDP+TDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+
Sbjct: 18  SASVVPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHS 77

Query: 77  NFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA---------QPQVT 136
           NFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A          P   
Sbjct: 78  NFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPF 137

Query: 137 VNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPP---------LSSSGPHS 196
               H +      +  G  H  GR+    + + ++ +W +S           LS  GP  
Sbjct: 138 FAPRHFAAGAFFRHGDGMLH--GRLGALVT-TTERRHWFESAALPVAPSSRLLSQLGPVI 197

Query: 197 GVHNN--NTNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSY 256
                   T    AL ++N RL R N  L+ ELAHM+KLY+DIIYFVQNHV+PVAPS   
Sbjct: 198 APARRAAATPEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVRPVAPS--- 257

Query: 257 QYSTTSLLSDGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAA---- 316
                +    G  V+  RPP           GK  +S+++  +  +  ++++   A    
Sbjct: 258 --PAAAAALHGLGVL--RPPP--------AGGKAPASEVRGASGRSATSSSSLTVAEDQP 317

Query: 317 -------TKSFVTILEEQQQQQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNN----- 354
                   ++   I+ E         TKLFGV + S        AS              
Sbjct: 318 TLLALRLPRTTEKIINEVSGGNGGGSTKLFGVHLSSADEQTSSGASRKRSPPQEQPPTSP 377

BLAST of Clc05G22280 vs. ExPASy TrEMBL
Match: A0A6J1IW73 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111481146 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 5.3e-159
Identity = 299/355 (84.23%), Postives = 313/355 (88.17%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNT 180
           VTVNQHHQS+SPLG+ N GFYH+P RVSISPSDSDD  NWCDSPPLSS+  ++ V N   
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNNNNNVFN--- 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TSLLS
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TSLLS 240

Query: 241 DGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEEQQQ 300
           DGFPV Q R PNHH   +H TN KQVS Q   V   TPNNN N N ++KSFVTI+EE   
Sbjct: 241 DGFPVGQ-RQPNHHLLGYHPTNAKQVSGQTHFV-GGTPNNN-NNNGSSKSFVTIVEE--- 300

Query: 301 QQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
                KTKLFGV++QSKKR+HPEY SNNI KE NNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 ----PKTKLFGVSLQSKKRVHPEYGSNNIAKE-NNKARLVLEKDDLGLNLMPPSA 339

BLAST of Clc05G22280 vs. ExPASy TrEMBL
Match: A0A6J1FJH2 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111445920 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 7.7e-158
Identity = 299/355 (84.23%), Postives = 312/355 (87.89%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNT 180
           VTVNQHHQS+SPLG+ N GFYH+P RVSISPSDSDD  NWCDSPPLSS+      +NN  
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNN----NNNFN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TSLLS
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TSLLS 240

Query: 241 DGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEEQQQ 300
           DGFPV Q R PNHH   +H TN KQVS Q   V A TPNNN   N ++KSFVTI+EE   
Sbjct: 241 DGFPVGQ-RQPNHHLLGYHPTNAKQVSGQTHFV-AGTPNNN---NGSSKSFVTIVEE--- 300

Query: 301 QQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
                KTKLFGV++QSKKR+HPEY SNNI KE NNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 ----PKTKLFGVSLQSKKRVHPEYGSNNIVKE-NNKARLVLEKDDLGLNLMPPSA 336

BLAST of Clc05G22280 vs. ExPASy TrEMBL
Match: A0A0A0L4W8 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 7.2e-156
Identity = 298/358 (83.24%), Postives = 309/358 (86.31%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFP-GRVSISPSDSDDQNN-WCDSPPLSSSGPHSGVHNN 180
           VTVNQHHQ HSPL   NPGFYHFP  R+SISPSDSDDQNN WCDSP           +NN
Sbjct: 121 VTVNQHHQPHSPL---NPGFYHFPTARLSISPSDSDDQNNYWCDSP---------SPNNN 180

Query: 181 NTNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TTS 240
           N NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TTS
Sbjct: 181 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTS 240

Query: 241 LLSDGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEE 300
           LLSDGFPVV  R PNH+HH+HH  + +QVSSQI+           N    TKSFVTILEE
Sbjct: 241 LLSDGFPVV--RQPNHYHHHHH--HHQQVSSQIR-----------NNTVGTKSFVTILEE 300

Query: 301 QQQQQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
             +QQQQTKTKLFGVAIQSKKRLHPEY ++N    NNNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 --EQQQQTKTKLFGVAIQSKKRLHPEYGNSN-SNNNNNKARLVLEKDDLGLNLMPPSA 328

BLAST of Clc05G22280 vs. ExPASy TrEMBL
Match: A0A6J1IFM1 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111476507 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 1.0e-154
Identity = 289/355 (81.41%), Postives = 306/355 (86.20%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+ HK IPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLEPHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNT 180
               QHHQS SPL +NNPGFYHF GR SISPSDSDDQNNWCDSPPLSSSG ++  +NN +
Sbjct: 121 ----QHHQSLSPLALNNPGFYHFHGRGSISPSDSDDQNNWCDSPPLSSSGTNNNNNNNYS 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST     
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST----- 240

Query: 241 DGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEEQQQ 300
             FPVVQ +P +H   Y++ TN K+VS Q  LVT  TPNNN + N   KS V I+EEQQ 
Sbjct: 241 --FPVVQRQPNHHLVGYNNPTNAKRVSGQTHLVTG-TPNNNNDSN---KSLVRIVEEQQ- 300

Query: 301 QQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
                KTKLFGVAI SKKRLHPEYASNNIGKENNNKAR VLEKDDLGLNLMPPSA
Sbjct: 301 -----KTKLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSA 333

BLAST of Clc05G22280 vs. ExPASy TrEMBL
Match: A0A6J1F8Q8 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111443150 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.4e-154
Identity = 292/355 (82.25%), Postives = 304/355 (85.63%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+SHK IPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 121 VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNT 180
               QH QS SPL +NNPGFYHF  R SISPSDSDDQNNWCDSPPLSSSG      NNN 
Sbjct: 121 ----QHQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGT-----NNNN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLS 240
           NSV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST     
Sbjct: 181 NSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST----- 240

Query: 241 DGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKNAATKSFVTILEEQQQ 300
             FPVVQ +P +H   Y++ TN KQVS Q  LVTA TPNNN N N   KSFV I+EEQQ 
Sbjct: 241 --FPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTA-TPNNNNNNN---KSFVRIVEEQQ- 300

Query: 301 QQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVLEKDDLGLNLMPPSA 356
                KTKLFGVAI SKKRLHPEYASNNIGKENNNKAR VLEKDDLGLNLMPPSA
Sbjct: 301 -----KTKLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSA 328

BLAST of Clc05G22280 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 322.8 bits (826), Expect = 3.7e-88
Identity = 195/373 (52.28%), Postives = 236/373 (63.27%), Query Frame = 0

Query: 1   MALMLDNCEG----------VLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA+M++N  G           L+     KA+PAPFLTKTYQLVDDP+TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQ--PQ--VTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPL 180
           IHRRKT+Q  PQ       HH +   +  +   F+  P     +P   ++ + WCD  P 
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTP---EEDHYWCDDSP- 180

Query: 181 SSSGPHSGVHNNNTNS-VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPV 240
             S P       +T + VTALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVKPV
Sbjct: 181 -PSRPRVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPV 240

Query: 241 APSNSYQYSTTSLLSDGFPVVQHRPPNHHHHYHHLTNGKQVSSQIQLVTATTPNNNTNKN 300
           APSN+  Y     LS      Q + P    +Y+  T           V AT  N   +  
Sbjct: 241 APSNNSSY-----LSSFLQKQQQQQPPTLDYYNTAT-----------VNATNLNALNSSP 300

Query: 301 AATKSFVTILEE----QQQQQQQTKTKLFGVAIQSKKRLHPEYASNNIGKENNNKARLVL 355
             ++S +T+LE+       Q    KTKLFGV++ S K+    ++      +  +K RLVL
Sbjct: 301 PTSQSSITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSHHFS------DQTSKTRLVL 346

BLAST of Clc05G22280 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 185.7 bits (470), Expect = 7.1e-47
Identity = 104/212 (49.06%), Postives = 132/212 (62.26%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           + +++PAPFL+KTYQLVDD STD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRK-----TAQPQVTVNQHHQSHS 136
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK     TA   V V    +S+S
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPSESNS 126

Query: 137 PLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNTNSVTALSEDNE 196
             G                    DD  +   S P SS  P S       N V  LS +NE
Sbjct: 127 GGG--------------------DDHGSSSTSSPGSSKNPGS-----VENMVADLSGENE 186

Query: 197 RLRRSNNMLMSELAHMKKLYNDIIYFVQNHVK 224
           +L+R NN L SELA  KK  ++++ F+  H+K
Sbjct: 187 KLKRENNNLSSELAAAKKQRDELVTFLTGHLK 193

BLAST of Clc05G22280 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.7e-46
Identity = 106/218 (48.62%), Postives = 137/218 (62.84%), Query Frame = 0

Query: 16  DSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 75
           DS ++IP PFLTKTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 76  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLG- 135
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK +QP +       + +    
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAAS 170

Query: 136 ----INNPGFYHFPGRVSISPSDS-DDQNNWCDSPPLSSSGPHSGV-----HNNNTNSVT 195
                  P   H      +SPS+S ++Q    +S P +++    GV         T+  T
Sbjct: 171 AVTVAAVPVVAHI-----VSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTT 230

Query: 196 A--LSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQN 221
           A  L E+NERLR+ N  L  E+  +K LY +I   + N
Sbjct: 231 APELVEENERLRKDNERLRKEMTKLKGLYANIYTLMAN 263

BLAST of Clc05G22280 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 177.6 bits (449), Expect = 1.9e-44
Identity = 99/219 (45.21%), Postives = 134/219 (61.19%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           S ++IP PFLTKT+ LV+D S D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLGIN 136
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK       +   HQ+     + 
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK-------ITTTHQT-----VV 135

Query: 137 NPGFYHFPGRVSISPSDSDDQNNWCDSPPLSSSGPHSGVHNNNTNSVTALS----EDNER 196
            P        + +SPS+S + NN  ++  +SSS      H   T     LS    E+NE+
Sbjct: 136 APSSEQRNQTMVVSPSNSGEDNN--NNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENEK 195

Query: 197 LRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSY 232
           LR  N  L  EL  MK + ++I   + N+V       SY
Sbjct: 196 LRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSY 220

BLAST of Clc05G22280 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 160.6 bits (405), Expect = 2.5e-39
Identity = 71/110 (64.55%), Postives = 89/110 (80.91%), Query Frame = 0

Query: 20  AIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 79
           ++P PFL+KTY +V+DP+TD IVSW   + +F+VW PPEF+RDLLP YFKHNNFSSFVRQ
Sbjct: 48  SLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQ 107

Query: 80  LNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQS 130
           LNTYGFRK+ PDRWEFANE F +G+KHLL +I RRK+ Q   + + + QS
Sbjct: 108 LNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQS 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897232.14.2e-17487.16heat stress transcription factor B-4-like [Benincasa hispida][more]
KAG6608467.12.2e-15984.51Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022982267.11.1e-15884.23heat stress transcription factor B-4-like [Cucurbita maxima][more]
XP_022940239.11.6e-15784.23heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 h... [more]
XP_004141262.24.3e-15582.96heat stress transcription factor B-4 [Cucumis sativus] >KAE8649863.1 hypothetica... [more]
Match NameE-valueIdentityDescription
Q9C6355.3e-8752.28Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q7XHZ01.7e-6954.79Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX87.9e-6758.02Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U942.8e-6444.30Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R81.0e-5841.05Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
A0A6J1IW735.3e-15984.23heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FJH27.7e-15884.23heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A0A0L4W87.2e-15683.24HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 ... [more]
A0A6J1IFM11.0e-15481.41heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1F8Q81.4e-15482.25heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G46264.13.7e-8852.28heat shock transcription factor B4 [more]
AT4G36990.17.1e-4749.06heat shock factor 4 [more]
AT4G11660.12.7e-4648.62winged-helix DNA-binding transcription factor family protein [more]
AT5G62020.11.9e-4445.21heat shock transcription factor B2A [more]
AT4G17750.12.5e-3964.55heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..203
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 150..187
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..191
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 12..354
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 63..75
score: 78.68
coord: 25..48
score: 47.38
coord: 76..88
score: 84.62
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 5.1E-60
score: 215.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 3.2E-32
score: 111.0
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..116
e-value: 1.5E-37
score: 130.2
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 12..354
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc05G22280.1Clc05G22280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding