Clc05G16860 (gene) Watermelon (cordophanus) v2

Overview
NameClc05G16860
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionAuxin-responsive GH3 family protein
LocationClcChr05: 24957861 .. 24961518 (-)
RNA-Seq ExpressionClc05G16860
SyntenyClc05G16860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAAACCCACTATGCTTCCTTTTTTCCTCCCCACATCTGAAATCCATCCCACCATCATTTGTAAACACACACACACAAAAACACACACACACACACATACACACACACACACACACAAACACCCTTCTCTTAATTACTCTTCCTTTTCCTCTCTCTTTCTTAACTATCCTTTTCAAACTGCAAAAACACAACAACAACAACAACTACTACTACTACAACAAACAACATCATGCCTGAAGAAGCTCTTCAAAACTCCTCATTTCTCTCCACCACTGAATACTACAAACTGTTAGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCAACTCCAGCGTCGCGTCCTCACCGAGATCCTCTCTCGCAATGCCAACGTCGAGTACCTCCGTCGACATGGCCTCCGTGGCGCCACCGACCGTAATACCTTCAAAAACACCCTTCCTCTCATCTCCTATGAGGATATTCAACCTGATATCGACCGTATTGCCAATGGGGACTCCTCTCCCATCCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACCAGGTGGGTCTCTATTTCCTCTAATCCTCTCTGTTTTTCTTGTTTCTTTTTTTTTCTCTTGCCTTGGAATGTGAATGAAAAACCCTTAATTTGACTTAAACTTTTATGTTTTCAAGTTTAAGTAAATGCCGTCTTGTTTACCGTGATATCCACCCGCTTCCAACTTTTTTTGAGTTTCCTTATACTTTAACTCGCAACAAAAGTCCTATTTTTAAATTGAGGTAAATGTCAACTAGTTAATTACCATAACCCATCCATTTTAAATTTTTAGAAATTCATCCAACTTGACTTTGATTTCAACAACAAATCCAAAATAAGTGGTGTCATATTGAAATCGCTAAATCATATCATTTGTTTACTGAAAATATCCATTCAAAAGCTTTCTTAATGGGTTGGGTAAATGTAAAATGTCATATGCTTAACTGAAATCTACCCACTTAAATATTTAGATTCAATGACAATTGATGTGAGTTTTACTGAAAATCCATCAACTAAAACTTTTTTAAGGTCAATAATATTAATTACAAAACAAACAAACCCGAAAGAAGACTGCAATTGATTTTGCATTCGAATGAAGGTTTTGAGTTTAGGGAGGATGATAATTAAACTAACCAATGGTTGGTGTCCAGCTCGGGAACGTCGGGAGGAGAGAGAAAACTGATGCCAACAATAGAGGAAGAGCTAGAAAGAAGATCATTACTGTACAGTTTACTAATGCCAGTAATGAGCCAATTCGTAGGAGGGTTAGAGAAAGGCAAAGGAATGTACTTCTTATTCGTAAAATCAGAATCAAAAACCCCAAGTGGATTACTAGCTCGACCTGTATTAACAAGCTATTACAAAAGCCCTCATTTCAAAAACCGCCCATTTGACCCCTACACCAATTACACAAGCCCAAACGAAGCCATCCTCTGTTCCGACGCCTACCAGAGCATGTACTCTCAGCTCCTCTGCGGCCTCCTCCACCGTGCCGACGTCCTCCGTGTCGGCGCCGTCTTTGCCTCCGGCTTCATCCGCGCTATCCGCTTCTTGGAGAAGCACTTTTCATTGCTCTGCCACGACATTCGAACAGGGACTCTGAACCCGGAAATCACGGACCAGGGGATTCGTGAATCGTTGAGGAAAATTCTGAAACCGGACCCCAAATTGGCGGATTTTGTGGAAGGGGAGTGTAGTAAGAAGAGTTGGGAAGGGATTATAAGTAGGATTTGGCCGAATACGAAGTATGTGGATGTGATTGTAACAGGGACAATGTCGCAGTATATAAGGACACTGGATTATTACAGTAATGGGTTGCCGATTGTTTGTACAATGTATGCTTCGTCGGAGTGTTATTTTGGGGTGAATTTGAAGCCGATGTGTAAGCCGGAGGAAGTGGCGTATACGCTAATACCAACAATGGCGTATTTTGAGTTCCTTCCTGTGAATAGGAATAAAGGGTTGAGTGAGAAGGAGTTGCAAAAGGAGCTTGTGGATCTTGTTGATGTTCAGCTTGGTCAGGAGTATGAGCTCGTTGTTACTACCTATGCAGGTCAGCAATTTATTTATTTATTATTATTTTTTTGTGGGAATTCGGGACGGAGTCGGAAAGAAGGTGACTTTTCCACTCATTTCAATTTGTATCTAATAGATCTTCCATAAATTTCAATTTTGTGTACCGGTTATTTACAGCAAATCTTTTATACGTGAAAAGTAAAGTTTCGTTTTTATAAAACTAAACTTATGCTAAATCAAACATAATTCAACTAGTATGAGAGAATATTGTTGACTAAAAATTCTTTGTAGTTTATATATATATATATATATATATATATATATAATAAGGCTTAATTTCTCTCAATTTTGCCACTAATAAATCATTGAATTACTTTTTTTAATTAATTTTCGAAATTTGACTTAGTTTTTTAAAAACACTAATAGAAAGTGTTTAACTATTTTCATCTAGTGTTTATATGTTTAATTTTTTAGAAATAAAAACTAAAACCAAGAAACCAACCATTACAAATAGGGTCTTAGTTTTTAGTGTTTGAAAATTAAGTTTATATACATAATTTCTCTATCTTGTTATTTATTTTTAGGAAAATAAATAGAAATTCAAGAAAAAAAGAAGTAATTTTAAAAAACGGTTGGATGTTTTTCTCAAAATCGAAAAACTAAATAGTTATCAAACCAAGCTTAAGATTTTAAATCTTCCTCTTTCTAGACTCTAATTCGAAACCAAGCATTAAATATAACACATGATGATGATGATGTTTGTATGTTTGATGTCTATGTCTTTTGATTTCAGGATTGTATAGGTATAGAGTGGGAGATGTACTAAGAGTGGCAGGGTTCAAGAACAATGCACCGCAATTCAACTTCATTTGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAAGTTGAGTTACAAAATGCTGTCAAAAATGCATTAAACCATTTGATTCCATTTGATGTCACATTGAGTGAGTACACAAGCTGTGTAGACACTTCGAAAATCCCAGGCCATTATGTACTCTATTGGGAGCTAACTATGAATAATGGAATCAAAACAACACCAATTCCACCCTCTGTCTTTGAGGATTGTTGCCTCACAATTGAAGAGTCTCTCAACGCCGTGTACCGTCAGGGTCGGGTCTCCGATAAGTCCATCGGCCCCCTAGAGATCAAGATCGTCGAGACCGGGACGTTTGACAAGCTCATGGACTATGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAGACACCAAGATGTGTTAAGTATGAACCCATTATTCAACTATTGAATTCAAGGGTTGTGTCAAATTACTTCAGCCCCAAGTGCCCTAAATGGGTTCCAGGTTATAAGCAGTGGAATTGAAATGGAAATTTTAATTGGATTATTGTATCAATGGGGAATATTTAAACACACCCTAGAGATTTTATGGTTTCTTCTCTAATCTTGTTCTTGATATTGCATCCTAATTTCTCAAATTTTTAGCAGCTCTTGGAAGTGGGTTTAGGCTATTTTCAACTTGATAGACTGTGTAC

mRNA sequence

TATAAACCCACTATGCTTCCTTTTTTCCTCCCCACATCTGAAATCCATCCCACCATCATTTGTAAACACACACACACAAAAACACACACACACACACATACACACACACACACACACAAACACCCTTCTCTTAATTACTCTTCCTTTTCCTCTCTCTTTCTTAACTATCCTTTTCAAACTGCAAAAACACAACAACAACAACAACTACTACTACTACAACAAACAACATCATGCCTGAAGAAGCTCTTCAAAACTCCTCATTTCTCTCCACCACTGAATACTACAAACTGTTAGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCAACTCCAGCGTCGCGTCCTCACCGAGATCCTCTCTCGCAATGCCAACGTCGAGTACCTCCGTCGACATGGCCTCCGTGGCGCCACCGACCGTAATACCTTCAAAAACACCCTTCCTCTCATCTCCTATGAGGATATTCAACCTGATATCGACCGTATTGCCAATGGGGACTCCTCTCCCATCCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACCAGCTCGGGAACGTCGGGAGGAGAGAGAAAACTGATGCCAACAATAGAGGAAGAGCTAGAAAGAAGATCATTACTGTACAGTTTACTAATGCCAGTAATGAGCCAATTCGTAGGAGGGTTAGAGAAAGGCAAAGGAATGTACTTCTTATTCGTAAAATCAGAATCAAAAACCCCAAGTGGATTACTAGCTCGACCTGTATTAACAAGCTATTACAAAAGCCCTCATTTCAAAAACCGCCCATTTGACCCCTACACCAATTACACAAGCCCAAACGAAGCCATCCTCTGTTCCGACGCCTACCAGAGCATGTACTCTCAGCTCCTCTGCGGCCTCCTCCACCGTGCCGACGTCCTCCGTGTCGGCGCCGTCTTTGCCTCCGGCTTCATCCGCGCTATCCGCTTCTTGGAGAAGCACTTTTCATTGCTCTGCCACGACATTCGAACAGGGACTCTGAACCCGGAAATCACGGACCAGGGGATTCGTGAATCGTTGAGGAAAATTCTGAAACCGGACCCCAAATTGGCGGATTTTGTGGAAGGGGAGTGTAGTAAGAAGAGTTGGGAAGGGATTATAAGTAGGATTTGGCCGAATACGAAGTATGTGGATGTGATTGTAACAGGGACAATGTCGCAGTATATAAGGACACTGGATTATTACAGTAATGGGTTGCCGATTGTTTGTACAATGTATGCTTCGTCGGAGTGTTATTTTGGGGTGAATTTGAAGCCGATGTGTAAGCCGGAGGAAGTGGCGTATACGCTAATACCAACAATGGCGTATTTTGAGTTCCTTCCTGTGAATAGGAATAAAGGGTTGAGTGAGAAGGAGTTGCAAAAGGAGCTTGTGGATCTTGTTGATGTTCAGCTTGGTCAGGAGTATGAGCTCGTTGTTACTACCTATGCAGGATTGTATAGGTATAGAGTGGGAGATGTACTAAGAGTGGCAGGGTTCAAGAACAATGCACCGCAATTCAACTTCATTTGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAAGTTGAGTTACAAAATGCTGTCAAAAATGCATTAAACCATTTGATTCCATTTGATGTCACATTGAGTGAGTACACAAGCTGTGTAGACACTTCGAAAATCCCAGGCCATTATGTACTCTATTGGGAGCTAACTATGAATAATGGAATCAAAACAACACCAATTCCACCCTCTGTCTTTGAGGATTGTTGCCTCACAATTGAAGAGTCTCTCAACGCCGTGTACCGTCAGGGTCGGGTCTCCGATAAGTCCATCGGCCCCCTAGAGATCAAGATCGTCGAGACCGGGACGTTTGACAAGCTCATGGACTATGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAGACACCAAGATGTGTTAAGTATGAACCCATTATTCAACTATTGAATTCAAGGGTTGTGTCAAATTACTTCAGCCCCAAGTGCCCTAAATGGGTTCCAGGTTATAAGCAGTGGAATTGAAATGGAAATTTTAATTGGATTATTGTATCAATGGGGAATATTTAAACACACCCTAGAGATTTTATGGTTTCTTCTCTAATCTTGTTCTTGATATTGCATCCTAATTTCTCAAATTTTTAGCAGCTCTTGGAAGTGGGTTTAGGCTATTTTCAACTTGATAGACTGTGTAC

Coding sequence (CDS)

ATGCCTGAAGAAGCTCTTCAAAACTCCTCATTTCTCTCCACCACTGAATACTACAAACTGTTAGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCAACTCCAGCGTCGCGTCCTCACCGAGATCCTCTCTCGCAATGCCAACGTCGAGTACCTCCGTCGACATGGCCTCCGTGGCGCCACCGACCGTAATACCTTCAAAAACACCCTTCCTCTCATCTCCTATGAGGATATTCAACCTGATATCGACCGTATTGCCAATGGGGACTCCTCTCCCATCCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACCAGCTCGGGAACGTCGGGAGGAGAGAGAAAACTGATGCCAACAATAGAGGAAGAGCTAGAAAGAAGATCATTACTGTACAGTTTACTAATGCCAGTAATGAGCCAATTCGTAGGAGGGTTAGAGAAAGGCAAAGGAATGTACTTCTTATTCGTAAAATCAGAATCAAAAACCCCAAGTGGATTACTAGCTCGACCTGTATTAACAAGCTATTACAAAAGCCCTCATTTCAAAAACCGCCCATTTGACCCCTACACCAATTACACAAGCCCAAACGAAGCCATCCTCTGTTCCGACGCCTACCAGAGCATGTACTCTCAGCTCCTCTGCGGCCTCCTCCACCGTGCCGACGTCCTCCGTGTCGGCGCCGTCTTTGCCTCCGGCTTCATCCGCGCTATCCGCTTCTTGGAGAAGCACTTTTCATTGCTCTGCCACGACATTCGAACAGGGACTCTGAACCCGGAAATCACGGACCAGGGGATTCGTGAATCGTTGAGGAAAATTCTGAAACCGGACCCCAAATTGGCGGATTTTGTGGAAGGGGAGTGTAGTAAGAAGAGTTGGGAAGGGATTATAAGTAGGATTTGGCCGAATACGAAGTATGTGGATGTGATTGTAACAGGGACAATGTCGCAGTATATAAGGACACTGGATTATTACAGTAATGGGTTGCCGATTGTTTGTACAATGTATGCTTCGTCGGAGTGTTATTTTGGGGTGAATTTGAAGCCGATGTGTAAGCCGGAGGAAGTGGCGTATACGCTAATACCAACAATGGCGTATTTTGAGTTCCTTCCTGTGAATAGGAATAAAGGGTTGAGTGAGAAGGAGTTGCAAAAGGAGCTTGTGGATCTTGTTGATGTTCAGCTTGGTCAGGAGTATGAGCTCGTTGTTACTACCTATGCAGGATTGTATAGGTATAGAGTGGGAGATGTACTAAGAGTGGCAGGGTTCAAGAACAATGCACCGCAATTCAACTTCATTTGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAAGTTGAGTTACAAAATGCTGTCAAAAATGCATTAAACCATTTGATTCCATTTGATGTCACATTGAGTGAGTACACAAGCTGTGTAGACACTTCGAAAATCCCAGGCCATTATGTACTCTATTGGGAGCTAACTATGAATAATGGAATCAAAACAACACCAATTCCACCCTCTGTCTTTGAGGATTGTTGCCTCACAATTGAAGAGTCTCTCAACGCCGTGTACCGTCAGGGTCGGGTCTCCGATAAGTCCATCGGCCCCCTAGAGATCAAGATCGTCGAGACCGGGACGTTTGACAAGCTCATGGACTATGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAGACACCAAGATGTGTTAAGTATGAACCCATTATTCAACTATTGAATTCAAGGGTTGTGTCAAATTACTTCAGCCCCAAGTGCCCTAAATGGGTTCCAGGTTATAAGCAGTGGAATTGA

Protein sequence

MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGYKQWN
Homology
BLAST of Clc05G16860 vs. NCBI nr
Match: XP_038896877.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida])

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 574/609 (94.25%), Postives = 589/609 (96.72%), Query Frame = 0

Query: 1   MPEEALQNSSFL-STTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYL 60
           MPEE  Q SSFL STTEYYKLLEKNKKTLQFIEDVTANAD +QRRVLTEIL+RNA+VEYL
Sbjct: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60

Query: 61  RRHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           RRHGL GATDR TFK TLPLISYEDIQPDI+RIANGDSSPILCSSPISEFLTSSGTSGGE
Sbjct: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLT 180
           RKLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLF+KSESKTPSGLLARPVLT
Sbjct: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180

Query: 181 SYYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGF 240
           SYYKS HFK+RPFDPYTNYTSPN+AILCSD+YQSMY+QLLCGLLHR DVLRVGAVFASGF
Sbjct: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWE 300
           IRAIRFLEKHF LLCHDIRTGTLN EITDQGIRESLRKILKPDPKLADFVEGEC KK W 
Sbjct: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360
           GIISRIWPNTKYVDVIVTGTMSQYI+TLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE
Sbjct: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360

Query: 361 EVAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV 420
           EVAYTLIPTMAYFEFLPVNR+KGLS+KELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV
Sbjct: 361 EVAYTLIPTMAYFEFLPVNRSKGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 480
           GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 480

Query: 481 SCVDTSKIPGHYVLYWELTM-NNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSI 540
           SCVDTSKIPGHYVLYWELT+ NN IKTTPIPPSVFEDCC TIEESLNAVYRQGRVSDKSI
Sbjct: 481 SCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSI 540

Query: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600
           GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600

Query: 601 WVPGYKQWN 608
           WVPG+KQWN
Sbjct: 601 WVPGHKQWN 609

BLAST of Clc05G16860 vs. NCBI nr
Match: KAG6592005.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 556/607 (91.60%), Postives = 582/607 (95.88%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEEA Q SSFLSTTEYYKLL++NKKTLQFIEDVTANA Q+QRRVL EILSRNA+VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL GATD  TFKNT+P+ISYEDIQ DI+RIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKNTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSDAYQSMY+QLLCGL HR DVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKHF LLCHDIRTGTLNP+ITDQ IRESL+K LKPDPKLADF+EGECSKKSW G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPAE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNRN  L+EKELQ ELVDLVDVQLGQEYELVVTTYAGLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           C DTSKIPGHYVLYWEL++ NG KTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSV-NGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600

Query: 601 PGYKQWN 608
           PG+KQW+
Sbjct: 601 PGHKQWS 606

BLAST of Clc05G16860 vs. NCBI nr
Match: KAG7024880.1 (Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 555/607 (91.43%), Postives = 582/607 (95.88%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEEA Q SSFLSTTEYYKLL++NKKTLQFIEDVTANA Q+QRRVL EILSRNA+VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL GATD  TFKNT+P+ISYEDIQ DI+RIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKNTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVM+QF+GGLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMNQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSDAYQSMY+QLLCGL HR DVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKHF LLCHDIRTGTLNP+ITDQ IRESL+K LKPDPKLADF+EGECSKKSW G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPAE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNRN  L+EKELQ ELVDLVDVQLGQEYELVVTTYAGLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           C DTSKIPGHYVLYWEL++ NG KTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSV-NGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600

Query: 601 PGYKQWN 608
           PG+KQW+
Sbjct: 601 PGHKQWS 606

BLAST of Clc05G16860 vs. NCBI nr
Match: XP_022935867.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 555/607 (91.43%), Postives = 581/607 (95.72%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEEA Q SSFLSTTEYYKLL++NKKTLQFIEDVTANA Q+QRRVL EILSRNA+VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL GATD  TFK T+P+ISYEDIQ DI+RIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKKTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSDAYQSMY+QLLCGL HR DVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKHF LLCHDIRTGTLNP+ITDQ IRESL+K LKPDPKLADF+EGECSKKSW G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPSE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNRN  L+EKELQ ELVDLVDVQLGQEYELVVTTYAGLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           C DTSKIPGHYVLYWEL++ NG KTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSV-NGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600

Query: 601 PGYKQWN 608
           PG+KQW+
Sbjct: 601 PGHKQWS 606

BLAST of Clc05G16860 vs. NCBI nr
Match: XP_023535071.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 554/607 (91.27%), Postives = 581/607 (95.72%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEEA Q SSFLSTTEYYKLL++NKKTLQFIEDVTANA Q+QRRVL EILSRNA+VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RH L GATD  TFKNT+P+ISYEDIQ DI+RIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHSLAGATDPKTFKNTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSDAYQSMY+QLLCGL HR DVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKHF LLCHDIRTGTLNP+ITDQ IRESL+K LKPDPKLADF+EGECSKKSW G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPAE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNRN  L+EKELQ ELVDLVDVQLGQEYELVVTTYAGLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           C DTSKIPGHYVLYWEL++ NG KTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSV-NGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR+VSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRIVSNYFSPKCPKWV 600

Query: 601 PGYKQWN 608
           PG+KQW+
Sbjct: 601 PGHKQWS 606

BLAST of Clc05G16860 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 6.5e-289
Identity = 482/597 (80.74%), Postives = 533/597 (89.28%), Query Frame = 0

Query: 20  LLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATDRNTFKNTLPL 79
           L EKNK  LQFIEDVT NAD +QRRVL EILSRNA+VEYL+RHGL G TDR TFK+ +P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 80  ISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSL 139
           ++YEDIQP+I+RIANGD S +LCS+PISEFLTSSGTSGGERKLMPTIEEEL+RRSLLYSL
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 140 LMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYT 199
           LMPVM QFV GL+KGKGMYFLF+KSESKTP GL ARPVLTSYYKS HFKNRP+DPYTNYT
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYT 196

Query: 200 SPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRT 259
           SPN+ ILCSD+YQSMYSQ+LCGL    +VLRVGAVFASGFIRAI+FLEKH+  L  DIRT
Sbjct: 197 SPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRT 256

Query: 260 GTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGT 319
           GTL+ EITD  +RE++ +ILKPDPKLADFVE EC K SW+GII+R+WPNTKYVDVIVTGT
Sbjct: 257 GTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 316

Query: 320 MSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNR 379
           MSQYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP MAYFEFLPV+R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 376

Query: 380 NKG----------LSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFK 439
           N G          L+EKE Q+ELVDLVDV+LGQEYELVVTTYAGLYRYRVGDVL VAGFK
Sbjct: 377 NSGVTSSISLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 436

Query: 440 NNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPG 499
           NNAPQF+FICRKNV LSIDSDKTDEVELQNAVKNA+ HL+PFD +LSEYTS  DTS IPG
Sbjct: 437 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 500 HYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETG 559
           HYVL+WEL +N     TPIPPSVFEDCCLTIEESLN+VYRQGRVSDKSIGPLEIK+VE+G
Sbjct: 497 HYVLFWELCLNG---NTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 556

Query: 560 TFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGYKQW 607
           TFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPKCPKW PG+KQW
Sbjct: 557 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQW 609

BLAST of Clc05G16860 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 5.9e-282
Identity = 473/595 (79.50%), Postives = 525/595 (88.24%), Query Frame = 0

Query: 22  EKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATDRNTFKNTLPLIS 81
           +KNK+ LQ IE++T+NADQ+QR+VL EIL+RNA+VEYLRRH L G TDR TFKN +P+I+
Sbjct: 19  QKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVIT 78

Query: 82  YEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 141
           YEDI+P+I+RIANGD SPIL S PISEFLTSSGTSGGERKLMPTIEEEL+RRSLLYSLLM
Sbjct: 79  YEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 142 PVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSP 201
           PVMSQFV GLE GKGMYFLF+KSESKTP GL ARPVLTSYYKS HFK RP+DPYTNYTSP
Sbjct: 139 PVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSP 198

Query: 202 NEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRTGT 261
           NE ILCSD+YQSMYSQ+LCGL    +VLRVGAVFASGFIRAI+FLEKH+  L  DIRTGT
Sbjct: 199 NETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGT 258

Query: 262 LNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGTMS 321
           L+  ITD  +RE++ KILKP PKLADFVE EC K SW+GII+R+WPNTKYVDVIVTGTMS
Sbjct: 259 LSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 322 QYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRNK 381
           QYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP+MAYFEFLPV+RN 
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 378

Query: 382 G----------LSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNN 441
           G          L+EKE Q+ELVDLVDV+LGQEYELVVTTYAGL RYRVGD+LRV GFKN 
Sbjct: 379 GVTNSINLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNK 438

Query: 442 APQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPGHY 501
           APQF+FICRKNV LSIDSDKTDEVELQNAVKNA+ HL+PFD +LSEYTS  DTS IPGHY
Sbjct: 439 APQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHY 498

Query: 502 VLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTF 561
           VL+WEL ++     TPIPPSVFEDCCL +EES N VYRQGRVSDKSIGPLEIKIVE GTF
Sbjct: 499 VLFWELCLDG---NTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 558

Query: 562 DKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGYKQW 607
           DKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPKCPKWVPG+KQW
Sbjct: 559 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQW 609

BLAST of Clc05G16860 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 7.5e-245
Identity = 405/583 (69.47%), Postives = 484/583 (83.02%), Query Frame = 0

Query: 26  KTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATD-RNTFKNTLPLISYED 85
           + L+FIE VTANA Q+QRRVL EIL++NA  EYLRR+G+ G+ D  + F+  +PL++YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 86  IQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMPVM 145
           +QPDI RIANGD+SPI    PISEFLTSSGTSGGERKLMPTI +E+ RRSLLYSLLMPVM
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 146 SQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSPNEA 205
           SQ V GL+KGK MY LFVK+ES+TP GL ARPVLTSYY+S  F +RP DPYT+YTSP+EA
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 206 ILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRTGTLNP 265
           ILC D+YQSMY+QLLCGL+HRADVLRVGAVFASGF+RAI FLEKH++ LCHDIRTG L+P
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 266 EITDQGIRESLRKILKPDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGTMSQYI 325
           EITD+ +R+++ ++L+ DP LAD +E EC++ SWEGII R+WP TKY+DVIVTGTMSQYI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 326 RTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRNKGLS 385
            TL++Y  GLP+ CTMYASSECYFG+NL PMCKP +VAYTLIPTM Y+EFLPVN N   +
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 386 EKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRKNVA 445
           E    ++LVDLVDV+LG EYELVVTTY+GLYRYRVGDVLRVAGFKN AP F+F+ R+NVA
Sbjct: 382 EAS-HRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVA 441

Query: 446 LSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPGHYVLYWELTMNNGIK 505
           LS+DSDKTDE EL  AV  A+ HL PF  +L EYTS  D + IPGHYVL+WEL       
Sbjct: 442 LSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAG---- 501

Query: 506 TTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAISLGAS 565
           +T +P SVFE+CCL++EE+LN+VYRQGR  D+SIGPLEI++V  GTFDKLMDYAIS GAS
Sbjct: 502 STAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGAS 561

Query: 566 INQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGYKQWN 608
           INQYK PRCV+  P+++LL++RV   YFSPKCPKW PG KQWN
Sbjct: 562 INQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQWN 599

BLAST of Clc05G16860 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 1.2e-229
Identity = 396/591 (67.01%), Postives = 468/591 (79.19%), Query Frame = 0

Query: 23  KNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATD--RNTFKNTLPLI 82
           ++++ L+ IE VT NA Q QRRVL EIL++NA  EYLRR G+ G        F+   PL+
Sbjct: 22  EHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLV 81

Query: 83  SYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLL 142
           +YEDI PD+ RIANGD+SPIL   P+SEFLTSSGTSGGERKLMPTIEEE+ERRS LYSLL
Sbjct: 82  TYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLL 141

Query: 143 MPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTS 202
           MPVMS+ V GL+KGK MY  FVKSE +TP GL ARPVLTS+Y+S +F  RP DPYT YTS
Sbjct: 142 MPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTS 201

Query: 203 PNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRTG 262
           P+EA+LC DAYQSMY+QL+CGL+HRADVLRVGAVFASGF+RAIRFLEKH+  LC DIR G
Sbjct: 202 PDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAG 261

Query: 263 TLNPEITDQGIRESLRKILK-PDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGT 322
            L+  +TD  +R ++ ++L+  DP LAD +E EC++ SW+GII R+WP+TKY+DVIVTG 
Sbjct: 262 ELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTGA 321

Query: 323 MSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVN- 382
           M+QYI TL++Y  GLP+ CTMYASSECYFG+NL PMCKP EVAYTLIPTM YFEFLPVN 
Sbjct: 322 MAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPVNS 381

Query: 383 -RNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNF 442
             N   + +   + LVDLVDV+LG EYELVVTTY+GLYRYRVGDVLRVAGFKN AP F F
Sbjct: 382 GANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMFAF 441

Query: 443 ICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDT-SKIPGHYVLYWE 502
           + RKNVALSIDSDKTDE EL  AV  A+ HL PF  +L EYTS  DT + IPGHYVL+WE
Sbjct: 442 VRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYVLFWE 501

Query: 503 LTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMD 562
           L    G   TP+P SVFEDCCL +EE LN+VYRQ R +D+SIGPLEI++V  GTFDKLMD
Sbjct: 502 LRSPAG--GTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGTFDKLMD 561

Query: 563 YAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW-VPGYKQW 607
           YA+S GASINQYK PRCV+  P+++LL+ RV + YFSPKCPKW   G KQW
Sbjct: 562 YALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQW 610

BLAST of Clc05G16860 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 1.0e-225
Identity = 384/581 (66.09%), Postives = 460/581 (79.17%), Query Frame = 0

Query: 22  EKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATDRNTFKNTLPLIS 81
           EK+ K L+FIE++T NAD +Q  +L EIL+RNA+ EYLRR  L GATDR+TFK  +P+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 82  YEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 141
           YED+QP+I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+RR LLYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 142 PVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSP 201
           PVM+ +V GL+KGKGMYFLFVKSE+KTP GL ARPVLTSYYKS HF++RP+DPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 202 NEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRTGT 261
           NEAILC D++QSMY+Q+LCGLL R  VLRVGAVFASG +RAIRFL+ H+S   HDI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 262 LNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGTMS 321
           L+ EITD  IR+ +  ILKPDP LA+F+  EC   +WE II+RIWPNTKY+DVIVTG M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 322 QYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRNK 381
           QYI TL+YYS GLP+ CTMYASSECYFG+NL PM KP EV+YT++P MAYFEF+P+   K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 382 GLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRK 441
                      V+LVDV +G+EYELVVTTYAGL RYRVGD+LRV GF N+APQF+F+ RK
Sbjct: 377 A----------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRK 436

Query: 442 NVALSIDSDKTDEVELQNAVKNALNHL-IPFDVTLSEYTSCVDTSKIPGHYVLYWELTMN 501
           NV LSIDSDKTDE ELQ AV+NA + L       ++EYTS  DTS IPGHYVLYWEL + 
Sbjct: 437 NVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVR 496

Query: 502 NGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAIS 561
           +G +           CCL +EESLN+VYRQ RV+D S+GPLEI++V  GTF++LMDYAIS
Sbjct: 497 DGARQP--SHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAIS 556

Query: 562 LGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVP 602
            GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P
Sbjct: 557 RGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTP 585

BLAST of Clc05G16860 vs. ExPASy TrEMBL
Match: A0A6J1F6T2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111442645 PE=3 SV=1)

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 555/607 (91.43%), Postives = 581/607 (95.72%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEEA Q SSFLSTTEYYKLL++NKKTLQFIEDVTANA Q+QRRVL EILSRNA+VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL GATD  TFK T+P+ISYEDIQ DI+RIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKKTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSDAYQSMY+QLLCGL HR DVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKHF LLCHDIRTGTLNP+ITDQ IRESL+K LKPDPKLADF+EGECSKKSW G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPSE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNRN  L+EKELQ ELVDLVDVQLGQEYELVVTTYAGLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           C DTSKIPGHYVLYWEL++ NG KTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSV-NGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600

Query: 601 PGYKQWN 608
           PG+KQW+
Sbjct: 601 PGHKQWS 606

BLAST of Clc05G16860 vs. ExPASy TrEMBL
Match: A0A6J1IM75 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucurbita maxima OX=3661 GN=LOC111476851 PE=3 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 553/607 (91.10%), Postives = 579/607 (95.39%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEEA Q SSFLSTTEYYKLL++NKKTLQFIEDVTANA Q+QRRVL EILSRNA+VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQIQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL GATD  TFKNT+P+ISYEDIQ DI+RIANGD SPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDSKTFKNTIPVISYEDIQHDINRIANGDFSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKS HFK+RPFDPYTNYTSPNEAILCSDAYQSMY+QLLCGL HR DVLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKHF LLCHDIRTGTLNP+ITDQ IRESL+K LKPDPKLADF+EGECSKKSW G
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWHG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           II+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP E
Sbjct: 301 IITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPAE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           V+YTLIPTMAYFEFLPVNRN  L+EKELQ ELVDLVDVQLGQEYELVVTTYAGLYRYRVG
Sbjct: 361 VSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYTS
Sbjct: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           C DTSKIPGHYVLYWEL++ NG KTT IPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CADTSKIPGHYVLYWELSV-NGAKTTLIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRAVSNYFSPKCPKWV 600

Query: 601 PGYKQWN 608
           PG+KQW+
Sbjct: 601 PGHKQWS 606

BLAST of Clc05G16860 vs. ExPASy TrEMBL
Match: A0A5A7V8A5 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold977G00060 PE=3 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 559/606 (92.24%), Postives = 575/606 (94.88%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEE  Q SSFLSTTEYYKLLEKNKKTLQFIEDVT NADQ+QRRVLTEILSRNA VEYLR
Sbjct: 1   MPEETPQKSSFLSTTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL GATD  TFKNTLPLISYEDIQPDI+RIANGD+SPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKSPHFKNRPFDPYTNYTSPNEAILCSD+YQSMY+QLLCGLLHR DVLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKH++LLCHDIRTGTLN EITDQGIRES+RKILK D KLADFVEGEC KK W G
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           IISRIWPNTKYVDVIVTGTMSQYI TLDYYSNGLPIVCTMYASSECYFGVNLKPMCKP E
Sbjct: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           VAYTLIPTMAYFEFLPVNR+K   EKEL+KELVDLVDV+LG EYELVVTTY+GLYRYRVG
Sbjct: 361 VAYTLIPTMAYFEFLPVNRSK---EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           D+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL HLIPFDVTLSEYTS
Sbjct: 421 DILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           CVDTSKIPGHYVLYWELT NN IK+TPIPPSVFEDCC  IEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CVDTSKIPGHYVLYWELTPNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPKWV 600

Query: 601 PGYKQW 607
           P  KQW
Sbjct: 601 P--KQW 601

BLAST of Clc05G16860 vs. ExPASy TrEMBL
Match: A0A1S3BYK9 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103494980 PE=3 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 559/606 (92.24%), Postives = 575/606 (94.88%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEE  Q SSFLSTTEYYKLLEKNKKTLQFIEDVT NADQ+QRRVLTEILSRNA VEYLR
Sbjct: 1   MPEETPQKSSFLSTTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL GATD  TFKNTLPLISYEDIQPDI+RIANGD+SPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKSPHFKNRPFDPYTNYTSPNEAILCSD+YQSMY+QLLCGLLHR DVLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKH++LLCHDIRTGTLN EITDQGIRES+RKILK D KLADFVEGEC KK W G
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           IISRIWPNTKYVDVIVTGTMSQYI TLDYYSNGLPIVCTMYASSECYFGVNLKPMCKP E
Sbjct: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           VAYTLIPTMAYFEFLPVNR+K   EKEL+KELVDLVDV+LG EYELVVTTY+GLYRYRVG
Sbjct: 361 VAYTLIPTMAYFEFLPVNRSK---EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           D+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL HLIPFDVTLSEYTS
Sbjct: 421 DILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           CVDTSKIPGHYVLYWELT NN IK+TPIPPSVFEDCC  IEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CVDTSKIPGHYVLYWELTPNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPKWV 600

Query: 601 PGYKQW 607
           P  KQW
Sbjct: 601 P--KQW 601

BLAST of Clc05G16860 vs. ExPASy TrEMBL
Match: A0A0A0LAR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G431430 PE=3 SV=1)

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 559/606 (92.24%), Postives = 577/606 (95.21%), Query Frame = 0

Query: 1   MPEEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLR 60
           MPEE  QNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQ+QRRVLTEILSRNA VEYLR
Sbjct: 1   MPEETPQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL GATD  TFKNTLPLISYEDIQPDI+RIANGDSS ILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLF+KSESKTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFI 240
           YYKSPHFKNRPFDPYTNYTSPNEAILCSD+YQSMY+QLLCGLLHR DVLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RAIRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEG 300
           RAIRFLEKH++LLCHDIRTGTLN EITDQGIRES+RKILK D KLADFVEGEC KK W G
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWGG 300

Query: 301 IISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEE 360
           IISRIWPNTKYVDVIVTGTMSQYI TLDYYSNGLPIVCTMYASSECYFGVNLKPMCKP E
Sbjct: 301 IISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPGE 360

Query: 361 VAYTLIPTMAYFEFLPVNRNKGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVG 420
           VAYTLIPTMAYFEFLPVNR+K   EKEL+KELVDLVDV+LG EYELVVTTY+GLYRYRVG
Sbjct: 361 VAYTLIPTMAYFEFLPVNRSK---EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTS 480
           D+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL HLIPFDVTLSEYTS
Sbjct: 421 DILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYTS 480

Query: 481 CVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGP 540
           CVDTS IPGHYVLYWELT+NN IK+TPIPPSVFEDCC  IEESLNAVYRQGRVSDKSIGP
Sbjct: 481 CVDTSNIPGHYVLYWELTLNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSIGP 540

Query: 541 LEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWV 600
           LEIKIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SRVVSNYFSPKCPKWV
Sbjct: 541 LEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPKWV 600

Query: 601 PGYKQW 607
           P  KQW
Sbjct: 601 P--KQW 601

BLAST of Clc05G16860 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 994.2 bits (2569), Expect = 4.6e-290
Identity = 482/597 (80.74%), Postives = 533/597 (89.28%), Query Frame = 0

Query: 20  LLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATDRNTFKNTLPL 79
           L EKNK  LQFIEDVT NAD +QRRVL EILSRNA+VEYL+RHGL G TDR TFK+ +P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 80  ISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSL 139
           ++YEDIQP+I+RIANGD S +LCS+PISEFLTSSGTSGGERKLMPTIEEEL+RRSLLYSL
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 140 LMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYT 199
           LMPVM QFV GL+KGKGMYFLF+KSESKTP GL ARPVLTSYYKS HFKNRP+DPYTNYT
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYT 196

Query: 200 SPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRT 259
           SPN+ ILCSD+YQSMYSQ+LCGL    +VLRVGAVFASGFIRAI+FLEKH+  L  DIRT
Sbjct: 197 SPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRT 256

Query: 260 GTLNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGT 319
           GTL+ EITD  +RE++ +ILKPDPKLADFVE EC K SW+GII+R+WPNTKYVDVIVTGT
Sbjct: 257 GTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 316

Query: 320 MSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNR 379
           MSQYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP MAYFEFLPV+R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 376

Query: 380 NKG----------LSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFK 439
           N G          L+EKE Q+ELVDLVDV+LGQEYELVVTTYAGLYRYRVGDVL VAGFK
Sbjct: 377 NSGVTSSISLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 436

Query: 440 NNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPG 499
           NNAPQF+FICRKNV LSIDSDKTDEVELQNAVKNA+ HL+PFD +LSEYTS  DTS IPG
Sbjct: 437 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 500 HYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETG 559
           HYVL+WEL +N     TPIPPSVFEDCCLTIEESLN+VYRQGRVSDKSIGPLEIK+VE+G
Sbjct: 497 HYVLFWELCLNG---NTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 556

Query: 560 TFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGYKQW 607
           TFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPKCPKW PG+KQW
Sbjct: 557 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQW 609

BLAST of Clc05G16860 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 971.1 bits (2509), Expect = 4.2e-283
Identity = 473/595 (79.50%), Postives = 525/595 (88.24%), Query Frame = 0

Query: 22  EKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATDRNTFKNTLPLIS 81
           +KNK+ LQ IE++T+NADQ+QR+VL EIL+RNA+VEYLRRH L G TDR TFKN +P+I+
Sbjct: 19  QKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVIT 78

Query: 82  YEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 141
           YEDI+P+I+RIANGD SPIL S PISEFLTSSGTSGGERKLMPTIEEEL+RRSLLYSLLM
Sbjct: 79  YEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 142 PVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSP 201
           PVMSQFV GLE GKGMYFLF+KSESKTP GL ARPVLTSYYKS HFK RP+DPYTNYTSP
Sbjct: 139 PVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSP 198

Query: 202 NEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRTGT 261
           NE ILCSD+YQSMYSQ+LCGL    +VLRVGAVFASGFIRAI+FLEKH+  L  DIRTGT
Sbjct: 199 NETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGT 258

Query: 262 LNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGTMS 321
           L+  ITD  +RE++ KILKP PKLADFVE EC K SW+GII+R+WPNTKYVDVIVTGTMS
Sbjct: 259 LSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 322 QYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRNK 381
           QYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP+MAYFEFLPV+RN 
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNN 378

Query: 382 G----------LSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNN 441
           G          L+EKE Q+ELVDLVDV+LGQEYELVVTTYAGL RYRVGD+LRV GFKN 
Sbjct: 379 GVTNSINLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNK 438

Query: 442 APQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPGHY 501
           APQF+FICRKNV LSIDSDKTDEVELQNAVKNA+ HL+PFD +LSEYTS  DTS IPGHY
Sbjct: 439 APQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHY 498

Query: 502 VLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTF 561
           VL+WEL ++     TPIPPSVFEDCCL +EES N VYRQGRVSDKSIGPLEIKIVE GTF
Sbjct: 499 VLFWELCLDG---NTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 558

Query: 562 DKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGYKQW 607
           DKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPKCPKWVPG+KQW
Sbjct: 559 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQW 609

BLAST of Clc05G16860 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 784.3 bits (2024), Expect = 7.3e-227
Identity = 384/581 (66.09%), Postives = 460/581 (79.17%), Query Frame = 0

Query: 22  EKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATDRNTFKNTLPLIS 81
           EK+ K L+FIE++T NAD +Q  +L EIL+RNA+ EYLRR  L GATDR+TFK  +P+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 82  YEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 141
           YED+QP+I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+RR LLYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 142 PVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSP 201
           PVM+ +V GL+KGKGMYFLFVKSE+KTP GL ARPVLTSYYKS HF++RP+DPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 202 NEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRTGT 261
           NEAILC D++QSMY+Q+LCGLL R  VLRVGAVFASG +RAIRFL+ H+S   HDI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 262 LNPEITDQGIRESLRKILKPDPKLADFVEGECSKKSWEGIISRIWPNTKYVDVIVTGTMS 321
           L+ EITD  IR+ +  ILKPDP LA+F+  EC   +WE II+RIWPNTKY+DVIVTG M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 322 QYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRNK 381
           QYI TL+YYS GLP+ CTMYASSECYFG+NL PM KP EV+YT++P MAYFEF+P+   K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 382 GLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRK 441
                      V+LVDV +G+EYELVVTTYAGL RYRVGD+LRV GF N+APQF+F+ RK
Sbjct: 377 A----------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRK 436

Query: 442 NVALSIDSDKTDEVELQNAVKNALNHL-IPFDVTLSEYTSCVDTSKIPGHYVLYWELTMN 501
           NV LSIDSDKTDE ELQ AV+NA + L       ++EYTS  DTS IPGHYVLYWEL + 
Sbjct: 437 NVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVR 496

Query: 502 NGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAIS 561
           +G +           CCL +EESLN+VYRQ RV+D S+GPLEI++V  GTF++LMDYAIS
Sbjct: 497 DGARQP--SHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAIS 556

Query: 562 LGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVP 602
            GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P
Sbjct: 557 RGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTP 585

BLAST of Clc05G16860 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 770.4 bits (1988), Expect = 1.1e-222
Identity = 381/602 (63.29%), Postives = 469/602 (77.91%), Query Frame = 0

Query: 3   EEALQNSSFLSTTEYYKLLEKNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRH 62
           +  LQ+    +TT      EK+ K L+FIE++T N D +Q +VL EIL+RN+N EYL+R 
Sbjct: 4   DSPLQSRMVSATTS-----EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRF 63

Query: 63  GLRGATDRNTFKNTLPLISYEDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKL 122
            L G  DR TFK+ +P+++YED++P+I RI+NGD SPIL S PI+EFLTSSGTS GERKL
Sbjct: 64  DLDGVVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKL 123

Query: 123 MPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYY 182
           MPTIEE+L+RR LLYSLLMPVM+ +V GL+KGKG+YFLFVKSESKT  GL ARPVLTSYY
Sbjct: 124 MPTIEEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYY 183

Query: 183 KSPHFKNRPFDPYTNYTSPNEAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRA 242
           KS HFK RP+DPY  YTSPNEAILCSD+ QSMY+Q+LCGLL R +VLR+GAVFASG +RA
Sbjct: 184 KSDHFKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRA 243

Query: 243 IRFLEKHFSLLCHDIRTGTLNPEITDQGIRESLRKIL-KPDPKLADFVEGECSKKSWEGI 302
           I FL+ ++  L  DI TGTL+  I D  I+  + KIL KPD +LA+F+ G CS+++WEGI
Sbjct: 244 ISFLQNNWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGI 303

Query: 303 ISRIWPNTKYVDVIVTGTMSQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEV 362
           I++IWPNTKY+DVIVTG M+QYI TL+YYS GLP+ CTMYASSE YFG+NLKPMCKP EV
Sbjct: 304 ITKIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEV 363

Query: 363 AYTLIPTMAYFEFLPVNRN-KGLSEKEL-QKELVDLVDVQLGQEYELVVTTYAGLYRYRV 422
           +YT++P MAYFEFLP N +  G +E  L +  LV+L +V++G+EYELV+TTYAGLYRYRV
Sbjct: 364 SYTIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRV 423

Query: 423 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 482
           GD+LRV GF N+APQF FI RKNV LS++SDKTDE ELQ AV+NA          + EYT
Sbjct: 424 GDILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYT 483

Query: 483 SCVDTSKIPGHYVLYWELTMNNGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIG 542
           S  +T  IPGHYV+YWEL +        +   V   CCL +EESLN+VYRQ RV+DKSIG
Sbjct: 484 SYAETKTIPGHYVIYWEL-LGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIG 543

Query: 543 PLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 602
           PLEI++V  GTF++LMDYAIS GASINQYK PRCV + PI++LL+SRVVS +FSP  P W
Sbjct: 544 PLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHW 599

BLAST of Clc05G16860 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 752.7 bits (1942), Expect = 2.3e-217
Identity = 373/581 (64.20%), Postives = 462/581 (79.52%), Query Frame = 0

Query: 23  KNKKTLQFIEDVTANADQLQRRVLTEILSRNANVEYLRRHGLRGATDRNTFKNTLPLISY 82
           K+ K L+FIE++T N D +Q++V+ EILSRN++ EYL+R GL+G TDR TFK  +P++ Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 83  EDIQPDIDRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMP 142
           +D++P+I RIANGD S IL S PI+EFLTSSGTS GERKLMPTI+E+++RR LLYSLLMP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 143 VMSQFVGGLEKGKGMYFLFVKSESKTPSGLLARPVLTSYYKSPHFKNRPFDPYTNYTSPN 202
           VM+ +V GL+KGK +YFLFVK+ESKTP GL ARPVLTSYYKS  FK RP DPY  YTSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 203 EAILCSDAYQSMYSQLLCGLLHRADVLRVGAVFASGFIRAIRFLEKHFSLLCHDIRTGTL 262
           EAILC D+ QSMY+Q+LCGLL R +VLR+GAVFASG +RAI FL+ ++  L  DI TGTL
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 263 NPEITDQGIRESLRKIL-KPDPKLADFVEGECSK-KSWEGIISRIWPNTKYVDVIVTGTM 322
           +  I+D  I+ES+ KIL KPD +LADF+   C +  SWEGII++IWPNTKY+DVIVTG M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 323 SQYIRTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRN 382
           +QYI  L+YYS GLP+ CTMYASSE YFG+NLKPMCKP EV+YT++P MAYFEFLP   +
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP--HH 377

Query: 383 KGLSEKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICR 442
           +  +EK    ELV+L DV++G+EYELV+TTYAGL RYRVGD+L+V GF N+APQF F+ R
Sbjct: 378 EVPTEK---SELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRR 437

Query: 443 KNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPGHYVLYWELTMN 502
           KNV LSI+SDKTDE ELQ+AV+NA   L      + EYTS  +T  IPGHYV+YWEL + 
Sbjct: 438 KNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVK 497

Query: 503 NGIKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAIS 562
           +  +T P    V   CCL +EESLN+VYRQ RV+DKSIGPLEI++V+ GTF++LMDYAIS
Sbjct: 498 D--QTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAIS 557

Query: 563 LGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVP 602
            GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P
Sbjct: 558 RGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSP 591

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896877.10.0e+0094.25indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida][more]
KAG6592005.10.0e+0091.60Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7024880.10.0e+0091.43Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma sub... [more]
XP_022935867.10.0e+0091.43indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata][more]
XP_023535071.10.0e+0091.27indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LSQ46.5e-28980.74Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818295.9e-28279.50Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM57.5e-24569.47Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
Q60EJ61.2e-22967.01Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
O823331.0e-22566.09Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1F6T20.0e+0091.43indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1IM750.0e+0091.10indole-3-acetic acid-amido synthetase GH3.6 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A5A7V8A50.0e+0092.24Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BYK90.0e+0092.24indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103494... [more]
A0A0A0LAR60.0e+0092.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G431430 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G54510.14.6e-29080.74Auxin-responsive GH3 family protein [more]
AT4G27260.14.2e-28379.50Auxin-responsive GH3 family protein [more]
AT2G14960.17.3e-22766.09Auxin-responsive GH3 family protein [more]
AT4G37390.11.1e-22263.29Auxin-responsive GH3 family protein [more]
AT2G23170.12.3e-21764.20Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 31..576
e-value: 2.3E-203
score: 676.9
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 14..606
NoneNo IPR availablePANTHERPTHR31901:SF70BNAC03G14570D PROTEINcoord: 14..606

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc05G16860.1Clc05G16860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity