Clc05G12420 (gene) Watermelon (cordophanus) v2

BLAST of Clc05G12420 vs. NCBI nr
Match: XP_038886841.1 (LOW QUALITY PROTEIN: kinetochore protein NDC80 homolog [Benincasa hispida])

HSP 1 Score: 944.9 bits (2441), Expect = 3.5e-271
Identity = 513/601 (85.36%), Postives = 538/601 (89.52%), Query Frame = 0

Query: 6   RRRPKGSFNPPP--PPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQISAVN 65
           RRRPK SFNP    P   +FRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQISAVN
Sbjct: 83  RRRPKASFNPASTCPRPIKFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQISAVN 142

Query: 66  TINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKSLGY 125
           TINSSLK+LFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDL IVLKSLGY
Sbjct: 143 TINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLSIVLKSLGY 202

Query: 126 PFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLNSYV 185
           PFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNY+LANDS+S+++SNDVNIYVLNSY+
Sbjct: 203 PFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYSLANDSNSVVMSNDVNIYVLNSYL 262

Query: 186 QYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQREVLE 245
           QYIRGDDDSVEALDQEFMERLKK RDDA ESLK +E+EVKELEAKAEALRSDP+QREVLE
Sbjct: 263 QYIRGDDDSVEALDQEFMERLKKGRDDAFESLKELEKEVKELEAKAEALRSDPTQREVLE 322

Query: 246 KQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKKRVE 305
           KQQ LLEEDVKKFHTMI GYSEKMQEMKKILEEKE+ELEVKV ERNKICEENEELKKRVE
Sbjct: 323 KQQCLLEEDVKKFHTMITGYSEKMQEMKKILEEKERELEVKVHERNKICEENEELKKRVE 382

Query: 306 SQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAMECNQ 365
           SQLFNPRDVERMRRELQAVERDI +AEVSRN WEEKSWDLDAT+GNKFKELEA+AMECNQ
Sbjct: 383 SQLFNPRDVERMRRELQAVERDIGDAEVSRNVWEEKSWDLDATVGNKFKELEAIAMECNQ 442

Query: 366 AMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAKGST 425
           AM+R                                       LKLDNCHQY LNAKGST
Sbjct: 443 AMRR---------------------------------------LKLDNCHQYFLNAKGST 502

Query: 426 PADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIASLQ 485
           PADVMGIDYKTT+KPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKR+HIASLQ
Sbjct: 503 PADVMGIDYKTTVKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRHHIASLQ 562

Query: 486 SHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAKLVH 545
           SHINE ETQLISLK+EIRDYTLRCSTESKK+VEDV+QEAHNLDLVEREA ESLKNAKL H
Sbjct: 563 SHINEGETQLISLKNEIRDYTLRCSTESKKMVEDVQQEAHNLDLVEREAGESLKNAKLAH 622

Query: 546 QEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSSMPA 605
           QEAIKQGEEEIQ+CARELVSCVDSVS++KAFVRSKILDMK CLSNAAA ISEAFK+SMP 
Sbjct: 623 QEAIKQGEEEIQLCARELVSCVDSVSEHKAFVRSKILDMKGCLSNAAAGISEAFKNSMPV 644

BLAST of Clc05G12420 vs. NCBI nr
Match: XP_004149971.2 (kinetochore protein NDC80 homolog isoform X1 [Cucumis sativus] >KAE8650517.1 hypothetical protein Csa_009662 [Cucumis sativus])

HSP 1 Score: 942.6 bits (2435), Expect = 1.7e-270
Identity = 505/604 (83.61%), Postives = 536/604 (88.74%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           MKGT RRRPK SFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRP++ASIDMYKERAVQIS
Sbjct: 1   MKGTARRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
            VNTINSSLK+LFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDY PTKLEDDLPI+LKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVA+YNYNLANDS+ +   +DVNIYVLN
Sbjct: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SY+ Y+RGDDDSVEALDQEFMERLKK RDD+ ESLK +EEEVK LEAK EALRSDPSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKK+LEEKEKEL+VK++ERNKICEENEELKK
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKK 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQLFNPRDVERMRRELQAVERDI +AEVSRN+WEEKSWDLDATLGNKFKELEA+AME
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLD+CHQYVLNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDSCHQYVLNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPADVMGIDYKTT+KPALDSFAEDIRRSSM KLEELISLRQQSSENTAKIESKR+HIA
Sbjct: 421 GSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEELISLRQQSSENTAKIESKRDHIA 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSH+NE ETQLISLK E+RDY LRCSTESKK+VEDV+QEAHNLDLVE EA ESLKNAK
Sbjct: 481 SLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDVQQEAHNLDLVEGEAEESLKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           L HQEAIKQGEEEI MCAREL+SC+DSVSQYKA V  KI DM+SCLS AAA ISEAFK+S
Sbjct: 541 LAHQEAIKQGEEEIHMCARELLSCLDSVSQYKAVVSCKISDMESCLSKAAAGISEAFKNS 565

Query: 601 MPAE 605
           MPAE
Sbjct: 601 MPAE 565

BLAST of Clc05G12420 vs. NCBI nr
Match: XP_008439248.1 (PREDICTED: kinetochore protein NDC80 homolog [Cucumis melo] >XP_008439249.1 PREDICTED: kinetochore protein NDC80 homolog [Cucumis melo] >KAA0033664.1 kinetochore protein NDC80-like protein [Cucumis melo var. makuwa] >TYK22962.1 kinetochore protein NDC80-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 928.3 bits (2398), Expect = 3.3e-266
Identity = 499/604 (82.62%), Postives = 531/604 (87.91%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           MKGTGRRRPK SFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRP +ASIDMYKERAVQIS
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
            VNTINSSLK+LFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDY PTKLEDDLPI+LKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINKNILK PPAPHQWYQILALLHWLVQVA+YNYNLANDS+ + + +DVNIYVLN
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SY+ Y+RGDDDSVEALDQEFMERLKK RDD+ ESLK +EEEVK LEAK EALRSDPSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKK+LEEKEKELEVK++ERNKICEENEELKK
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELEVKMEERNKICEENEELKK 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQLFNPRDVERMRRELQAVERDI +AEVSRN+WEEKSWDLDATLGNKFKELEA+AME
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLD+CHQYVLNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDSCHQYVLNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPADVMGIDYK+T+KPALDSFAEDIRRSSM KLEELISLRQQSSENTAKI+SKRN IA
Sbjct: 421 GSTPADVMGIDYKSTVKPALDSFAEDIRRSSMMKLEELISLRQQSSENTAKIDSKRNQIA 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSHINE ET+LISLK E+RDY LRCSTESKK+VED +QEAHNLDLVE EA ESLKNAK
Sbjct: 481 SLQSHINEHETKLISLKDEMRDYILRCSTESKKMVEDFQQEAHNLDLVEGEAEESLKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           L +QEA KQ EEEI MCAREL+SC+DSVSQYKA V  KI DM+SCLS AAA ISEAFK+S
Sbjct: 541 LANQEATKQEEEEIHMCARELLSCLDSVSQYKAVVSCKISDMESCLSKAAAGISEAFKNS 565

Query: 601 MPAE 605
           MP E
Sbjct: 601 MPVE 565

BLAST of Clc05G12420 vs. NCBI nr
Match: XP_022984383.1 (kinetochore protein NDC80 homolog [Cucurbita maxima])

HSP 1 Score: 917.1 bits (2369), Expect = 7.7e-263
Identity = 492/607 (81.05%), Postives = 531/607 (87.48%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           MKG GRR PKGSFNPPPPPTPQFRRDSD SFASSRPSSIGM+RPAAASIDMYKERAVQIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
           AVNTINSSLK+LF+HSFN+TFKPFSPPSLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINK ILK PPAPHQWY ILALLHWLVQVAVYNYNLANDSDS + SNDVN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SYVQYIRGDDDSVEALDQEFMERLKK RDDA+ESL  +E EVKELEAKAEALRSDPSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQ++LEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQE  KICEENEELK 
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQ+FN RDVERMRRELQAVERDI +AEVSRN+WEEK+WD+DAT+GNKFKELEA+A E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLDNCHQY LNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDNCHQYFLNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPADVMG+DYKTTIKPALDSFAEDI++SSMTKLE+LISLRQQSSENTAKIES+R+HI 
Sbjct: 421 GSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLISLRQQSSENTAKIESRRSHIL 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSHI E ETQ+ SLK+EIRDYTLRCSTESKK+VEDV+ EAH++D+VEREA E LKNAK
Sbjct: 481 SLQSHIEEAETQVNSLKNEIRDYTLRCSTESKKMVEDVQLEAHSIDVVEREAGEILKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           L H+EAIKQGEEEI +CARELV+CVDSVSQYK FVRSKI DM+S LS  A  ISEA+K+S
Sbjct: 541 LKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKISDMRSNLSKTAVGISEAYKNS 568

Query: 601 MPAEQGT 608
           +PAE GT
Sbjct: 601 LPAELGT 568

BLAST of Clc05G12420 vs. NCBI nr
Match: XP_023553517.1 (kinetochore protein NDC80 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 914.8 bits (2363), Expect = 3.8e-262
Identity = 491/607 (80.89%), Postives = 530/607 (87.31%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           MKG GRR PKGSFNPPPPPTPQFRRDSD SFASSRPSSIGM+RPAAASIDMYKERAVQIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
           AVNTINSSLK+LF+HSFN+TFKPFSPPSLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINK ILK PPAPHQWY ILALLHWLVQVAVYNYNLANDSDS + SNDVN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SYVQYIRGDDDSVEALDQEFMERLKK RDDA+ESL  +E EVKELEAKAEALRSDPSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQ++LEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQE  KICEENEELK 
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQ+FN RDVERMRRELQAVERDI +AEVSRN+WEEK+WD+DAT+GNKFKELEA+A E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLDNCHQY LNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDNCHQYFLNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPADVMG+DYKTTIKPALDSFAEDI++SSMTKLE+LISLRQQSSENTAKIES+R+HI 
Sbjct: 421 GSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLISLRQQSSENTAKIESRRSHIV 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSHI E ETQ+ SLK+EIRDYTLRCSTESKK+VEDV+ EAH++D+VE EA E LKNAK
Sbjct: 481 SLQSHIEEVETQVNSLKNEIRDYTLRCSTESKKMVEDVQLEAHSIDVVESEAGEILKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           L H+EAIKQGEEEI +CARELV+CVDSVSQYK FVRSKI DM+S LS  A  ISEA+K+S
Sbjct: 541 LKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKISDMRSNLSKTAVGISEAYKNS 568

Query: 601 MPAEQGT 608
           +PAE GT
Sbjct: 601 LPAELGT 568

BLAST of Clc05G12420 vs. ExPASy Swiss-Prot
Match: Q9M1G5 (Kinetochore protein NDC80 homolog OS=Arabidopsis thaliana OX=3702 GN=NDC80 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 9.3e-110
Identity = 243/613 (39.64%), Postives = 381/613 (62.15%), Query Frame = 0

Query: 5   GRRRPKGSFN---PPPPPTPQFR------RDSDVSFASSRPSSIGMSRPAAASIDMYKER 64
           G+RR    F    PPPPP+ + +      RDSD SFASSRPSSIG+    A+      +R
Sbjct: 7   GKRRTTVGFGGAPPPPPPSIEQQRHLFNSRDSDASFASSRPSSIGLGGRGASD-----DR 66

Query: 65  AVQISAVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDY--TPTKLEDD 124
           +  I  +N        L +H+F ++ +    PS+KD++ET+  LL  LDY     K ++D
Sbjct: 67  SSMIRFINAF------LSTHNFPISIRGNPVPSVKDISETLKFLLSALDYPCDSIKWDED 126

Query: 125 LPIVLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSND 184
           L   LKS   PFKI K+ LK P  PH W  +LA++HWL ++A ++ +L ++S S+   N 
Sbjct: 127 LVFFLKSQKCPFKITKSSLKAPNTPHNWPTVLAVVHWLAELARFHQHLVSNSTSVPEDNS 186

Query: 185 VNIYVLNSYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALR 244
           +N + + S+  +IRG+DD V  LD +F+ +L+  +    E++   E+   ELEAK E+LR
Sbjct: 187 MNFFAIQSFGHFIRGEDDKVNDLDSQFLGKLEAEKTSVAETISGCEKISGELEAKLESLR 246

Query: 245 SDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICE 304
             PS++E LEK ++ LE DV KF T++  Y+++   M+K++EEK KEL+ K +ER +I  
Sbjct: 247 KGPSKKESLEKVKADLENDVNKFRTIVVEYTDRNPAMEKVVEEKAKELKAKEEERERISV 306

Query: 305 ENEELKKRVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKE 364
           EN+ELKK VE Q F+  DV RMRRELQAVERD+++AEV+R+ W++K+W+L++ + N+F +
Sbjct: 307 ENKELKKSVELQNFSAADVNRMRRELQAVERDVADAEVARDGWDQKAWELNSQIRNQFHQ 366

Query: 365 LEALAMECNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCH 424
           ++ LA++CNQA++R                                       LKLD   
Sbjct: 367 IQTLAIDCNQALRR---------------------------------------LKLD--I 426

Query: 425 QYVLNAKGSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIE 484
           Q+ +N +G TPA VMG+DYK+ +KPAL S  + I+ SS  K+EEL++L+   SE  +KIE
Sbjct: 427 QFAVNERGETPAAVMGVDYKSVVKPALCSLCDGIKGSSAEKVEELVTLQHHKSEMASKIE 486

Query: 485 SKRNHIASLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAV 544
           SKR+ + S+Q  IN+ E ++  +K E ++ + +C  E+K +VE V+ EA NL++VE+EA 
Sbjct: 487 SKRSLLGSIQLQINDLEEKMKLVKKETQELSTKCDLEAKTLVESVKAEALNLEVVEKEAA 546

Query: 545 ESLKNAKLVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARI 604
           E +K ++L  QEA+K+ EEE+Q CA +L + +DS+S+ K ++ SKI ++K+ +++ A+ +
Sbjct: 547 EFVKASELRLQEAVKESEEEVQACAAQLFALIDSISKQKEYMDSKISEIKTGVADTASAV 567

Query: 605 SEAFKSSMPAEQG 607
           SE +K++     G
Sbjct: 607 SEIYKANFKKNLG 567

BLAST of Clc05G12420 vs. ExPASy Swiss-Prot
Match: Q76I89 (Kinetochore protein NDC80 homolog OS=Gallus gallus OX=9031 GN=NDC80 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.7e-15
Identity = 120/557 (21.54%), Postives = 233/557 (41.83%), Query Frame = 0

Query: 75  HSFNVTFKPFSPPSLKD----LTETINLLLQCLDYTPTKLEDDLPIVLKSLGYPFKINKN 134
           ++ NVT K    PS+KD     T     L    +   +K E+++P V K LGYPF ++K+
Sbjct: 111 YAHNVTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKS 170

Query: 135 ILKNPPAPHQWYQILALLHWLVQ-VAVY------------NYNLANDSDSLILSNDVNI- 194
            +    APH W QI+A L WL     +Y              N   ++D  I+ N + + 
Sbjct: 171 SMYTVGAPHTWPQIVAALIWLTDCFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMD 230

Query: 195 YVLNSYVQYIRGDDDSVEALDQEFMERLK---KARDDALESL----KVMEEEVKELEAKA 254
           YV+  Y  +++G  D+ E LD E   +LK      +  +E L    K + EE+  LE + 
Sbjct: 231 YVVKCYEHFMKG-GDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKER 290

Query: 255 EALRSDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERN 314
           E   S+P +   L K +S  + DV+K+   +A        + + ++   +E+E    E  
Sbjct: 291 E---SEPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVE 350

Query: 315 KICEENEELKKRVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGN 374
            + +EN  L+   ++Q ++  D+ER+  E   +++ I++      A E + W+ +     
Sbjct: 351 AMKQENARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKYAR 410

Query: 375 KFKELEALAMECNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKL 434
             + +E    E ++  ++L L PIS       + +G                        
Sbjct: 411 NKEAIETQLAEYHKLARKLKLIPIS-----AENSKG------------------------ 470

Query: 435 DNCHQYVLNAKGSTPADVMGIDYKTTIKPAL----DSFAEDIRRSSMTKLEELISLRQQS 494
              H + ++       + + + Y+T IK  L    +   E+IR+++  K+    SL    
Sbjct: 471 ---HDFEIHFNPEAGPNCL-VKYRTQIKAPLMEIVNQTEEEIRKATQRKM----SLEDTL 530

Query: 495 SENTAKIESKRNHIASLQSHINE-DETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHN 554
            +  A +  K++ + +L+    + D+     LK            E +K   ++E    +
Sbjct: 531 EQVNAMVAEKKSSVKTLKEEAEKLDDLYHQKLK--------EAEEEEQKCANELELLEKH 590

Query: 555 LDLVEREAVESLKNAKLVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKS 602
             L+E    E L  A     +  +Q +  +Q    E     D++++    + + ++ ++ 
Sbjct: 591 KQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESRKAGDNLNRLLEVITTHVVSIEK 618

BLAST of Clc05G12420 vs. ExPASy Swiss-Prot
Match: Q4R630 (Kinetochore protein NDC80 homolog OS=Macaca fascicularis OX=9541 GN=NDC80 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.9e-15
Identity = 113/523 (21.61%), Postives = 236/523 (45.12%), Query Frame = 0

Query: 75  HSFNVTFKPFSPPSLKDLTETINLLLQCL----DYTPTKLEDDLPIVLKSLGYPFKINKN 134
           ++ NV+ K    PS+KD  +    L   L    +   TK E+++P + K LGYPF ++K+
Sbjct: 108 YAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKS 167

Query: 135 ILKNPPAPHQWYQILALLHWLVQVAVYNYNL-------------ANDSDSLILSNDVNI- 194
            +    APH W  I+A L WL+     +  +               +++  I+ N + + 
Sbjct: 168 SMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLD 227

Query: 195 YVLNSYVQYIRGDDDSVEALDQEFMERLKKARD-DA--LESLKVMEEEVKELEAKAEALR 254
           Y +  Y  ++ G  DS + ++ E   +LK   + DA  LESL+     + E  A+ E  R
Sbjct: 228 YTIKCYESFMSG-ADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQER 287

Query: 255 -SDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKIC 314
             +P++ E L K ++ L+ DV+K+      Y   ++    IL++K   L  ++      C
Sbjct: 288 EKEPNRLESLRKLKASLQGDVQKYQ----AYMSNLESHSAILDQKLNGLNEEIARVELEC 347

Query: 315 E----ENEELKKRVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLG 374
           E    EN  L+  +++Q ++  D+ER+  E   +++ I++      A ++K W+ +    
Sbjct: 348 ETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYA 407

Query: 375 NKFKELEALAMECNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLK 434
              + +E    E ++  ++L L P        +     +  E   +  +  +  + V LK
Sbjct: 408 RGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLK 467

Query: 435 --LDNCHQYVLNAKGSTPADVMGID--------YKTTIKPALDSFAEDIRRSSMTKLEEL 494
             L+   + +  A+       MG++          T  K ++ +  E++++      +++
Sbjct: 468 ELLNETEEEINKARNKK----MGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKI 527

Query: 495 ISLRQQSSENTAKIESKRNHIASLQSHINEDETQLIS-LKSEIRDYTL--RCSTESKKIV 554
               ++  +  +++ES   H   L+S +N+  ++ ++ L +  R+Y L  + +TE ++ V
Sbjct: 528 KEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKV 587

BLAST of Clc05G12420 vs. ExPASy Swiss-Prot
Match: O14777 (Kinetochore protein NDC80 homolog OS=Homo sapiens OX=9606 GN=NDC80 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.9e-14
Identity = 112/519 (21.58%), Postives = 235/519 (45.28%), Query Frame = 0

Query: 75  HSFNVTFKPFSPPSLKDLTETINLLLQCL----DYTPTKLEDDLPIVLKSLGYPFKINKN 134
           ++ NV+ K    PS+KD  +    L   L    +   TK E+++P + K LGYPF ++K+
Sbjct: 108 YAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKS 167

Query: 135 ILKNPPAPHQWYQILALLHWLVQVAVYNYNL-------------ANDSDSLILSNDVNI- 194
            +    APH W  I+A L WL+     +  +               +++  I+ N + + 
Sbjct: 168 SMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLD 227

Query: 195 YVLNSYVQYIRGDDDSVEALDQEFMERLKKARD-DA--LESLKVMEEEVKELEAKAEALR 254
           Y +  Y  ++ G  DS + ++ E   +LK   + DA  LESL+     + E  A+ E  R
Sbjct: 228 YTIKCYESFMSG-ADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQER 287

Query: 255 -SDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKIC 314
             +P++ E L K ++ L+ DV+K+      Y   ++    IL++K   L  ++      C
Sbjct: 288 EKEPNRLESLRKLKASLQGDVQKYQ----AYMSNLESHSAILDQKLNGLNEEIARVELEC 347

Query: 315 E----ENEELKKRVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLG 374
           E    EN  L+  +++Q ++  D+ER+  E   +++ I++      A ++K W+ +    
Sbjct: 348 ETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYA 407

Query: 375 NKFKELEALAMECNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLK 434
              + +E    E ++  ++L L P        +     +  E   +  +  +  + V LK
Sbjct: 408 RGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLK 467

Query: 435 --LDNCHQYV---LNAKGSTPADVMGIDYK-TTIKPALDSFAEDIRRSSMTKLEELISLR 494
             L+   + +   LN K      +  ++   T  K ++ +  E++++      +++    
Sbjct: 468 ELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAE 527

Query: 495 QQSSENTAKIESKRNHIASLQSHINEDETQLIS-LKSEIRDYTL--RCSTESKKIVED-- 554
           ++  +  +++ES   H   L+S +N+  ++ ++ L +  R+Y L  + +TE ++ V +  
Sbjct: 528 EEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNL 587

BLAST of Clc05G12420 vs. ExPASy Swiss-Prot
Match: Q9D0F1 (Kinetochore protein NDC80 homolog OS=Mus musculus OX=10090 GN=Ndc80 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.6e-13
Identity = 112/528 (21.21%), Postives = 236/528 (44.70%), Query Frame = 0

Query: 77  FNVTFKPFSPPSLKDLTETINLLLQCL----DYTPTKLEDDLPIVLKSLGYPFKINKNIL 136
           ++V+ K    PS K+  +    L   L    +   TK E+++P + K+LGYPF ++K+ +
Sbjct: 110 YSVSMKSLQAPSTKEFLKIFAFLYGFLCPSYELPGTKCEEEVPRIFKALGYPFTLSKSSM 169

Query: 137 KNPPAPHQWYQILALLHWLVQVAVYNYNL-------------ANDSDSLILSNDVNI-YV 196
               APH W  I+A L WL+     +  +               +++  I  N + + Y 
Sbjct: 170 YTVGAPHTWPHIVAALVWLIDCIKIDTAMKESSPLFDDGQLWGEETEDGIKHNKLFLEYT 229

Query: 197 LNSYVQYIRGDDDSVEALDQEFMERLK---KARDDALESL----KVMEEEVKELEAKAEA 256
              Y +++ G  DS E  D E   +LK   K     LESL    K + E++  LE + E 
Sbjct: 230 KKCYEKFMTG-ADSFEEEDAELQAKLKDLYKVDASKLESLEAENKALNEQIARLEEERE- 289

Query: 257 LRSDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKI 316
              +P++   L+K ++ L+ DV+ +   ++     +  +K+     ++E+    QE   +
Sbjct: 290 --REPNRLMSLKKLKASLQADVQNYKAYMSNLESHLAVLKQKSNSLDEEIGRVEQECETV 349

Query: 317 CEENEELKKRVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKF 376
            +EN  L+  V++Q ++  D+ER+  E   +++ I++      A +++ W+ +       
Sbjct: 350 KQENTRLQSIVDNQKYSVADIERINHEKNELQQTINKLTKDLEAEQQQMWNEELKYARGK 409

Query: 377 KELEALAMECNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLK--L 436
           + +EA   E ++  ++L L P        +     +  E   +  +  +  +   LK  L
Sbjct: 410 EAIEAQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRTQVYAPLKELL 469

Query: 437 DNCHQYV---LNAKGSTPADVMGID-YKTTIKPALDSFAEDIRRSSMTKLEELISLRQQS 496
           +   + +   LN K      +  ++  KT  K  +    E+I++      + +    ++ 
Sbjct: 470 NESEEEINKALNKKRHLEDTLEQLNTMKTESKNTVRMLKEEIQKLDDLHQQAVKEAEEKD 529

Query: 497 SENTAKIESKRNHIASLQSHINEDETQLI-SLKSEIRDY--TLRCSTESKKIVEDVEQEA 556
            ++ +++ES   H   L+S +N+  ++ +  L +  R+Y  T++ +TE ++ VE+  Q  
Sbjct: 530 KKSASELESLEKHKHLLESGVNDGLSEAMDELDAVQREYQLTVKTTTEERRKVENNLQR- 589

Query: 557 HNLDLVEREAVESLKNAKLVHQEAIKQGEEEIQMCARELVSCVDSVSQ 571
               L+E  A       K + +E  K   E  +  + +L+  +  +++
Sbjct: 590 ----LLEMVATHVGSLEKHLEEENAKADREYEEFMSEDLLENIREMAE 628

BLAST of Clc05G12420 vs. ExPASy TrEMBL
Match: A0A1S3AYB0 (kinetochore protein NDC80 homolog OS=Cucumis melo OX=3656 GN=LOC103484089 PE=3 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.6e-266
Identity = 499/604 (82.62%), Postives = 531/604 (87.91%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           MKGTGRRRPK SFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRP +ASIDMYKERAVQIS
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
            VNTINSSLK+LFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDY PTKLEDDLPI+LKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINKNILK PPAPHQWYQILALLHWLVQVA+YNYNLANDS+ + + +DVNIYVLN
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SY+ Y+RGDDDSVEALDQEFMERLKK RDD+ ESLK +EEEVK LEAK EALRSDPSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKK+LEEKEKELEVK++ERNKICEENEELKK
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELEVKMEERNKICEENEELKK 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQLFNPRDVERMRRELQAVERDI +AEVSRN+WEEKSWDLDATLGNKFKELEA+AME
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLD+CHQYVLNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDSCHQYVLNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPADVMGIDYK+T+KPALDSFAEDIRRSSM KLEELISLRQQSSENTAKI+SKRN IA
Sbjct: 421 GSTPADVMGIDYKSTVKPALDSFAEDIRRSSMMKLEELISLRQQSSENTAKIDSKRNQIA 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSHINE ET+LISLK E+RDY LRCSTESKK+VED +QEAHNLDLVE EA ESLKNAK
Sbjct: 481 SLQSHINEHETKLISLKDEMRDYILRCSTESKKMVEDFQQEAHNLDLVEGEAEESLKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           L +QEA KQ EEEI MCAREL+SC+DSVSQYKA V  KI DM+SCLS AAA ISEAFK+S
Sbjct: 541 LANQEATKQEEEEIHMCARELLSCLDSVSQYKAVVSCKISDMESCLSKAAAGISEAFKNS 565

Query: 601 MPAE 605
           MP E
Sbjct: 601 MPVE 565

BLAST of Clc05G12420 vs. ExPASy TrEMBL
Match: A0A5D3DHD6 (Kinetochore protein NDC80-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00670 PE=3 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.6e-266
Identity = 499/604 (82.62%), Postives = 531/604 (87.91%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           MKGTGRRRPK SFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRP +ASIDMYKERAVQIS
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
            VNTINSSLK+LFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDY PTKLEDDLPI+LKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINKNILK PPAPHQWYQILALLHWLVQVA+YNYNLANDS+ + + +DVNIYVLN
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SY+ Y+RGDDDSVEALDQEFMERLKK RDD+ ESLK +EEEVK LEAK EALRSDPSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKK+LEEKEKELEVK++ERNKICEENEELKK
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELEVKMEERNKICEENEELKK 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQLFNPRDVERMRRELQAVERDI +AEVSRN+WEEKSWDLDATLGNKFKELEA+AME
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLD+CHQYVLNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDSCHQYVLNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPADVMGIDYK+T+KPALDSFAEDIRRSSM KLEELISLRQQSSENTAKI+SKRN IA
Sbjct: 421 GSTPADVMGIDYKSTVKPALDSFAEDIRRSSMMKLEELISLRQQSSENTAKIDSKRNQIA 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSHINE ET+LISLK E+RDY LRCSTESKK+VED +QEAHNLDLVE EA ESLKNAK
Sbjct: 481 SLQSHINEHETKLISLKDEMRDYILRCSTESKKMVEDFQQEAHNLDLVEGEAEESLKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           L +QEA KQ EEEI MCAREL+SC+DSVSQYKA V  KI DM+SCLS AAA ISEAFK+S
Sbjct: 541 LANQEATKQEEEEIHMCARELLSCLDSVSQYKAVVSCKISDMESCLSKAAAGISEAFKNS 565

Query: 601 MPAE 605
           MP E
Sbjct: 601 MPVE 565

BLAST of Clc05G12420 vs. ExPASy TrEMBL
Match: A0A6J1JAC2 (kinetochore protein NDC80 homolog OS=Cucurbita maxima OX=3661 GN=LOC111482699 PE=3 SV=1)

HSP 1 Score: 917.1 bits (2369), Expect = 3.7e-263
Identity = 492/607 (81.05%), Postives = 531/607 (87.48%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           MKG GRR PKGSFNPPPPPTPQFRRDSD SFASSRPSSIGM+RPAAASIDMYKERAVQIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
           AVNTINSSLK+LF+HSFN+TFKPFSPPSLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINK ILK PPAPHQWY ILALLHWLVQVAVYNYNLANDSDS + SNDVN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SYVQYIRGDDDSVEALDQEFMERLKK RDDA+ESL  +E EVKELEAKAEALRSDPSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQ++LEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQE  KICEENEELK 
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQ+FN RDVERMRRELQAVERDI +AEVSRN+WEEK+WD+DAT+GNKFKELEA+A E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLDNCHQY LNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDNCHQYFLNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPADVMG+DYKTTIKPALDSFAEDI++SSMTKLE+LISLRQQSSENTAKIES+R+HI 
Sbjct: 421 GSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLISLRQQSSENTAKIESRRSHIL 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSHI E ETQ+ SLK+EIRDYTLRCSTESKK+VEDV+ EAH++D+VEREA E LKNAK
Sbjct: 481 SLQSHIEEAETQVNSLKNEIRDYTLRCSTESKKMVEDVQLEAHSIDVVEREAGEILKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           L H+EAIKQGEEEI +CARELV+CVDSVSQYK FVRSKI DM+S LS  A  ISEA+K+S
Sbjct: 541 LKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKISDMRSNLSKTAVGISEAYKNS 568

Query: 601 MPAEQGT 608
           +PAE GT
Sbjct: 601 LPAELGT 568

BLAST of Clc05G12420 vs. ExPASy TrEMBL
Match: A0A6J1E5Y9 (kinetochore protein NDC80 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430885 PE=3 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 6.0e-261
Identity = 486/607 (80.07%), Postives = 530/607 (87.31%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           MKG GRR PKGSFNPPPPPTPQFRRDSD SFASSRPSSIGM+RPAAASIDMYKERAVQIS
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
           AVNTINSSLK+LF+HSFN+TFKPFSPPSLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINK ILK PPAPHQWY ILALLHWLVQVAVYNYNLANDSDS + SNDVN+YVLN
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SYVQYIRGDDDSVEALDQEFMERLKK RDDA+ESL  +E EVKELEAKAEALRSDPSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQ++LEEDVKKFHTMI GYSEKMQEMKKILEEKEKELEVKVQE  KICEENEELK 
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIGGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQ+FN RDVERMRRELQAVERDI +AEVSRN+WEEK+WD+DAT+GNKFKELEA+A E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLDNCHQY LNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDNCHQYFLNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPADVMG+DYKTTIKP+LDSFAEDI++SSMTKLE+LISLRQQSSEN+AKIES+R+HI 
Sbjct: 421 GSTPADVMGVDYKTTIKPSLDSFAEDIKKSSMTKLEDLISLRQQSSENSAKIESRRSHIL 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSHI E ETQ+ SL++EIRDYTLRCSTESKK+VED++ EAH++D+VEREA E LKNAK
Sbjct: 481 SLQSHIEEGETQVNSLRNEIRDYTLRCSTESKKMVEDLQLEAHSIDVVEREAGELLKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           + H+EAIKQGEEEI +CARELV+CVDSVSQYK FVRSKI DM+S LS  A  ISEA+K+S
Sbjct: 541 VKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKISDMRSNLSKTAVGISEAYKNS 568

Query: 601 MPAEQGT 608
           +PAE GT
Sbjct: 601 LPAELGT 568

BLAST of Clc05G12420 vs. ExPASy TrEMBL
Match: A0A6J1D5D4 (kinetochore protein NDC80 homolog OS=Momordica charantia OX=3673 GN=LOC111017757 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 4.7e-242
Identity = 456/607 (75.12%), Postives = 515/607 (84.84%), Query Frame = 0

Query: 1   MKGTGRRRPKGSFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPAAASIDMYKERAVQIS 60
           M+G GRRRPK SFNPPPPPTPQFRRDSD SFASSRPSSIGM+R AAASID+YKERAVQ S
Sbjct: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60

Query: 61  AVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYTPTKLEDDLPIVLKS 120
           AVN INSSLK+LFSHSFNVTFKP +P SLKDLTETINLLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSNDVNIYVLN 180
           LGYPFKINK+ILK PPAPHQWY ILALLHWLVQVAVYN +LANDS S I +N VN+Y L 
Sbjct: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180

Query: 181 SYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALRSDPSQRE 240
           SYV YIRGDDDSVE LDQ+FMERL+K RD+ +ESL  ++++V+ELEA AEALR++PSQRE
Sbjct: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEANAEALRTEPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICEENEELKK 300
           VLEKQQS+LEEDVKKFHTMI GYSEK+QEMKKILEEKEKELEVK QE+ +ICEENEELK 
Sbjct: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQEKKRICEENEELKM 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKELEALAME 360
           RVESQ+FNPRDVERMRRELQAVERDI + EVSRNAWEEK+WDLDATLG+KFKELEA+AME
Sbjct: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360

Query: 361 CNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCHQYVLNAK 420
           CNQAM+R                                       LKLDNC QY+LNAK
Sbjct: 361 CNQAMRR---------------------------------------LKLDNCRQYILNAK 420

Query: 421 GSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIESKRNHIA 480
           GSTPA++MGIDYK+T+KPALDS A+DI++SSMTKLE+LISLRQQSSENTAK+ES+R +IA
Sbjct: 421 GSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLISLRQQSSENTAKMESRRKNIA 480

Query: 481 SLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAVESLKNAK 540
           SLQSHI+E ETQ+ SL++E +DY LRCSTE+KK+VEDV QEAHNLDL+EREA E LKNAK
Sbjct: 481 SLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVRQEAHNLDLMEREAGEILKNAK 540

Query: 541 LVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARISEAFKSS 600
           L HQEA+KQ EEEIQ+CAREL++ VDSVS+Y+  VRSKI DMKS LS  A  ISEA+K+S
Sbjct: 541 LKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKISDMKSNLSKTAIGISEAYKNS 568

Query: 601 MPAEQGT 608
           +PAE GT
Sbjct: 601 LPAEMGT 568

BLAST of Clc05G12420 vs. TAIR 10
Match: AT3G54630.1 (CONTAINS InterPro DOMAIN/s: Kinetochore protein Ndc80 (InterPro:IPR005550); Has 24780 Blast hits to 15608 proteins in 1321 species: Archae - 545; Bacteria - 2969; Metazoa - 12597; Fungi - 2181; Plants - 1581; Viruses - 39; Other Eukaryotes - 4868 (source: NCBI BLink). )

HSP 1 Score: 399.1 bits (1024), Expect = 6.6e-111
Identity = 243/613 (39.64%), Postives = 381/613 (62.15%), Query Frame = 0

Query: 5   GRRRPKGSFN---PPPPPTPQFR------RDSDVSFASSRPSSIGMSRPAAASIDMYKER 64
           G+RR    F    PPPPP+ + +      RDSD SFASSRPSSIG+    A+      +R
Sbjct: 7   GKRRTTVGFGGAPPPPPPSIEQQRHLFNSRDSDASFASSRPSSIGLGGRGASD-----DR 66

Query: 65  AVQISAVNTINSSLKDLFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDY--TPTKLEDD 124
           +  I  +N        L +H+F ++ +    PS+KD++ET+  LL  LDY     K ++D
Sbjct: 67  SSMIRFINAF------LSTHNFPISIRGNPVPSVKDISETLKFLLSALDYPCDSIKWDED 126

Query: 125 LPIVLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVAVYNYNLANDSDSLILSND 184
           L   LKS   PFKI K+ LK P  PH W  +LA++HWL ++A ++ +L ++S S+   N 
Sbjct: 127 LVFFLKSQKCPFKITKSSLKAPNTPHNWPTVLAVVHWLAELARFHQHLVSNSTSVPEDNS 186

Query: 185 VNIYVLNSYVQYIRGDDDSVEALDQEFMERLKKARDDALESLKVMEEEVKELEAKAEALR 244
           +N + + S+  +IRG+DD V  LD +F+ +L+  +    E++   E+   ELEAK E+LR
Sbjct: 187 MNFFAIQSFGHFIRGEDDKVNDLDSQFLGKLEAEKTSVAETISGCEKISGELEAKLESLR 246

Query: 245 SDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQERNKICE 304
             PS++E LEK ++ LE DV KF T++  Y+++   M+K++EEK KEL+ K +ER +I  
Sbjct: 247 KGPSKKESLEKVKADLENDVNKFRTIVVEYTDRNPAMEKVVEEKAKELKAKEEERERISV 306

Query: 305 ENEELKKRVESQLFNPRDVERMRRELQAVERDISEAEVSRNAWEEKSWDLDATLGNKFKE 364
           EN+ELKK VE Q F+  DV RMRRELQAVERD+++AEV+R+ W++K+W+L++ + N+F +
Sbjct: 307 ENKELKKSVELQNFSAADVNRMRRELQAVERDVADAEVARDGWDQKAWELNSQIRNQFHQ 366

Query: 365 LEALAMECNQAMKRLPLFPISLFIDCCHHLRGIWGAELKTDYSIVGKIGILVKLKLDNCH 424
           ++ LA++CNQA++R                                       LKLD   
Sbjct: 367 IQTLAIDCNQALRR---------------------------------------LKLD--I 426

Query: 425 QYVLNAKGSTPADVMGIDYKTTIKPALDSFAEDIRRSSMTKLEELISLRQQSSENTAKIE 484
           Q+ +N +G TPA VMG+DYK+ +KPAL S  + I+ SS  K+EEL++L+   SE  +KIE
Sbjct: 427 QFAVNERGETPAAVMGVDYKSVVKPALCSLCDGIKGSSAEKVEELVTLQHHKSEMASKIE 486

Query: 485 SKRNHIASLQSHINEDETQLISLKSEIRDYTLRCSTESKKIVEDVEQEAHNLDLVEREAV 544
           SKR+ + S+Q  IN+ E ++  +K E ++ + +C  E+K +VE V+ EA NL++VE+EA 
Sbjct: 487 SKRSLLGSIQLQINDLEEKMKLVKKETQELSTKCDLEAKTLVESVKAEALNLEVVEKEAA 546

Query: 545 ESLKNAKLVHQEAIKQGEEEIQMCARELVSCVDSVSQYKAFVRSKILDMKSCLSNAAARI 604
           E +K ++L  QEA+K+ EEE+Q CA +L + +DS+S+ K ++ SKI ++K+ +++ A+ +
Sbjct: 547 EFVKASELRLQEAVKESEEEVQACAAQLFALIDSISKQKEYMDSKISEIKTGVADTASAV 567

Query: 605 SEAFKSSMPAEQG 607
           SE +K++     G
Sbjct: 607 SEIYKANFKKNLG 567