Clc05G03250 (gene) Watermelon (cordophanus) v2

Overview
NameClc05G03250
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptiontRNA (Guanine-N(7)-)-methyltransferase
LocationClcChr05: 2236306 .. 2239342 (+)
RNA-Seq ExpressionClc05G03250
SyntenyClc05G03250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCAATCCAAAATAGTGAGGGAAAGTATAATTTAAATGAAAGAAATAGGAAAATATAAAAGAAGGTGATAGAGAGCGAATAGAAACAGCTCCACTTCGAGGAGGGGCAAAATGGCTTCGTCCCTTTGCTTGCATTTACAAACTTGCTCGCACCTCCGCTGCATTGCGGCTGCAAAGGCGGCGGCGCCGTCGATGGTCGCAACTGCAAACAAAAGCTTGTGTCCGTTTCTAAAACGACCATTTCTTCCAAACTTCTGTTTACCATCGGGCTCTTCCGCCGACCTTTGCGAGCGGCAACCCCTCCGGAGTCCGGAATTGGTGGCGTTGGAATATGCCGACCTTAATCTCTCTTATAAAATCTTCGAGGTTTGTGTCTATGTTCATAAATTTTGCCTCTAATTTCTGAAATCGTTGCCGAGTAATTGAAAATTGAATGTACTGATTAGAATTGATGTGCATTTTTCTTGGTTTTCGTATAATTGCAGGAGGCGGGCCACGTTAGAGTCAGGCAGCATGTTAATCCTCTTAGCTCTTCTTTCTCTGTATGTAATTTGAAGAGCAATTGAAGGAATTGTGGGTTTGAAATTTCAGCTTAGTGCTGGTTAATTGAAGGAAGGCTCTCCCTCTTTTACCTTTCAGGTCCCTACACCAGCACCAAACTGGGATGAAGTCTTCAAGGACCCCAAATTGCCCCTCATGGTCGATATAGGAAGTGGTGAGTGAGATGATGCATTTTCTGTTATATACCTATAAATATCTGTTTTTTTCACAATACCTACTTCTTTTGTATTCGAGCTACTTATGCATACCTGGATTTCATTGGACAATATTTGATTATCTATAACATTTGGCTGAATCCATGTCCGTTATGAACTGTTTGAGATTGAGAGTGATTTCAAAATGGTTAAAATCATAATTGTCATGTTCAAAATCTCTATGAAACATGCTTTTTATCATTCTAAATCAAATTTGATGGTATGAAAATAGTGTTTAGAAGTGTAAAATTAAACAATAAATTGATTTAAATTGATTAAAGACACAATGTTCAAGGAATGATTTTGAAGCTAACAAAAGTGATATTTTAAAATCACTCTCAAATATGGCATTATTATAGTCTGTCTCTTACCCATTATCTGCCAGCTTGGGTAAGCTAAACAGAGGATAGCCATGAAAAAATCTTTGAAGCTACTGCTTGATTTGAAAAAGAAGATCAAAAGACAGATATATTTAGAGATTCTGCCATTTTCTTAACCATGGTTTGATGATATTCAATTAACCAGCATGAAGAAGTCATAGAAACCAAGTAAATTGGGCTGGTAAGAAAATTGTATCAAACGATTGTTAATATCTTAAAAGGGAAATATTATCCTCAAGCATCATATTGATTACTGCATTCAGCGATCTTATTTACCTGGCACTTTGACTCAAAATTCAACTTGATATGCATGATAAAGCAACTCTAAATATGAAACAGTACGGCACATGTGGAGAAAAATACGCTATTCAATAGCAGCTATGAGCCATCCACCTTGATTTAAACATTGGGCTAGCTAGTGAAAGTTAATACAATATCAGTTACCTAAGTTGACCTGGAGAATTTACCTAGGAAAAGATTCTTGGTAAAAATATTATTGTTTCAAATGCTCCAATGGCCGAGCATATCACATGTATGCACGAAAATTATTTATCAGATATAATGTGATCTAATTTGCATCTTTACTCTTAAAAATGTTAGTTACAATTGATGAAAATCTTGATTATCTCTCAATTTTGGTTTTACTAGCCTCATTACATACTTTTTGTGGGATTCATGAGGCTGTTTTATACTTTCTTTGCCCATCAAGATTTTAACAGAACAAGTATTCCTATTCCCAGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAGACACCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAAGTACGGAACTGAAAATTTTCACTTTTGACGTACTCTTAATTGATCTGAACTATTTGATCCTCATTCTCTGGAGGCCTTAGTTTGTACTTCGCTTTCTTTTGTGCTCTTGCAGCTGGTCAAGCGTGCTGATATCTGGGTTAAAGAGTTGGGATTTAGCAATGTGTAAGGACCTTAACTTCATATCAGGTAGTGAAAAGCAAGATCACCTTTTCGCTATTGGTACATTAGTAATGAACTTGCTTTTTAAACTTGATTGCAGACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATTATATCTTCATATCCCGGACCCCTGATGCTGGTTTCCATACTGGTTAGTGCACAACCATTCATACTTTCATAGCACACTAACCCAAACAATTTAACCTGAACTTCCCCATTGATTCGATTCGATTAATCGACAAAGATAGAACTCACTTGTGTTTAATCTCATTGCAGTGCCCAGATCCCCATTTTAAGAAACGACATCATAAGCGAAGGGTTTTGCAGAAGCCTTTAGTAGAATCTATAGTAGACAGTTTAATGCCAGGAGGGCAGGTATATGATTTGGTTATTCGCCTAACATTGAATGTTCTTTTGCCTGTGCTGTTTCAACCTGATGTGATGCAATATGCAATATGATGATTATTCTATATGATTCAACAGGTGTTCATACAATCTGACGTGCTCGAAGTGGCAGATGACATGAGACTTTGGTTCGATTCCAAAGCCAATGTTCTCAAACACATCGACAAGCTTGACCGGAGTTTTTTGTGTGATAAAGATGGATGGTTGTTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAATTCATGCTGAATCAGAAGGATCAAACATTTATAGAAGAATGTATCAAAAGTATACTTGACAAAAGAATAACTTACTAAATGGATTGTTTAATCATAATCTGTTTGCATTTGAGTCATCATTGCCTGTTATTTCTTTTGAGGCCGTCTGCCTATTAGTGTTATTGGGAAGTTATATAATATTTTCTAGACATATATTTATTGCACGTTTATAAAAATAAGAAAAAACTAACTTTTCTTCTTC

mRNA sequence

ATCAATCCAAAATAGTGAGGGAAAGTATAATTTAAATGAAAGAAATAGGAAAATATAAAAGAAGGTGATAGAGAGCGAATAGAAACAGCTCCACTTCGAGGAGGGGCAAAATGGCTTCGTCCCTTTGCTTGCATTTACAAACTTGCTCGCACCTCCGCTGCATTGCGGCTGCAAAGGCGGCGGCGCCGTCGATGGTCGCAACTGCAAACAAAAGCTTGTGTCCGTTTCTAAAACGACCATTTCTTCCAAACTTCTGTTTACCATCGGGCTCTTCCGCCGACCTTTGCGAGCGGCAACCCCTCCGGAGTCCGGAATTGGTGGCGTTGGAATATGCCGACCTTAATCTCTCTTATAAAATCTTCGAGGAGGCGGGCCACGTTAGAGTCAGGCAGCATGTTAATCCTCTTAGCTCTTCTTTCTCTGTCCCTACACCAGCACCAAACTGGGATGAAGTCTTCAAGGACCCCAAATTGCCCCTCATGGTCGATATAGGAAGTGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAGACACCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAACTGGTCAAGCGTGCTGATATCTGGGTTAAAGAGTTGGGATTTAGCAATGTACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATTATATCTTCATATCCCGGACCCCTGATGCTGGTTTCCATACTGTGCCCAGATCCCCATTTTAAGAAACGACATCATAAGCGAAGGGTTTTGCAGAAGCCTTTAGTAGAATCTATAGTAGACAGTTTAATGCCAGGAGGGCAGGTGTTCATACAATCTGACGTGCTCGAAGTGGCAGATGACATGAGACTTTGGTTCGATTCCAAAGCCAATGTTCTCAAACACATCGACAAGCTTGACCGGAGTTTTTTGTGTGATAAAGATGGATGGTTGTTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAATTCATGCTGAATCAGAAGGATCAAACATTTATAGAAGAATGTATCAAAAGTATACTTGACAAAAGAATAACTTACTAAATGGATTGTTTAATCATAATCTGTTTGCATTTGAGTCATCATTGCCTGTTATTTCTTTTGAGGCCGTCTGCCTATTAGTGTTATTGGGAAGTTATATAATATTTTCTAGACATATATTTATTGCACGTTTATAAAAATAAGAAAAAACTAACTTTTCTTCTTC

Coding sequence (CDS)

ATGGCTTCGTCCCTTTGCTTGCATTTACAAACTTGCTCGCACCTCCGCTGCATTGCGGCTGCAAAGGCGGCGGCGCCGTCGATGGTCGCAACTGCAAACAAAAGCTTGTGTCCGTTTCTAAAACGACCATTTCTTCCAAACTTCTGTTTACCATCGGGCTCTTCCGCCGACCTTTGCGAGCGGCAACCCCTCCGGAGTCCGGAATTGGTGGCGTTGGAATATGCCGACCTTAATCTCTCTTATAAAATCTTCGAGGAGGCGGGCCACGTTAGAGTCAGGCAGCATGTTAATCCTCTTAGCTCTTCTTTCTCTGTCCCTACACCAGCACCAAACTGGGATGAAGTCTTCAAGGACCCCAAATTGCCCCTCATGGTCGATATAGGAAGTGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAGACACCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAACTGGTCAAGCGTGCTGATATCTGGGTTAAAGAGTTGGGATTTAGCAATGTACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATTATATCTTCATATCCCGGACCCCTGATGCTGGTTTCCATACTGTGCCCAGATCCCCATTTTAAGAAACGACATCATAAGCGAAGGGTTTTGCAGAAGCCTTTAGTAGAATCTATAGTAGACAGTTTAATGCCAGGAGGGCAGGTGTTCATACAATCTGACGTGCTCGAAGTGGCAGATGACATGAGACTTTGGTTCGATTCCAAAGCCAATGTTCTCAAACACATCGACAAGCTTGACCGGAGTTTTTTGTGTGATAAAGATGGATGGTTGTTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAATTCATGCTGAATCAGAAGGATCAAACATTTATAGAAGAATGTATCAAAAGTATACTTGA

Protein sequence

MASSLCLHLQTCSHLRCIAAAKAAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSADLCERQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFKDPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGSNIYRRMYQKYT
Homology
BLAST of Clc05G03250 vs. NCBI nr
Match: XP_038879912.1 (tRNA (guanine-N(7)-)-methyltransferase [Benincasa hispida])

HSP 1 Score: 581.3 bits (1497), Expect = 5.1e-162
Identity = 285/307 (92.83%), Postives = 296/307 (96.42%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAKAAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSADLCE 60
           MASSLCLHLQTCSHLR IAAAKAAAPS VATANK+L  FLKRPFLPNFCLPSGSSADLCE
Sbjct: 1   MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCE 60

Query: 61  RQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFKDPK 120
           RQPLRSPELVALEYADLNLS+KIFE++GHVR+RQHVNPLSSSFSVPTPAPNWDEVFKDP 
Sbjct: 61  RQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPT 120

Query: 121 LPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMFANA 180
           LPLMVDIG GSGRFLLWLAKRH DSRNFLGLEIR+KLVKRADIWVKELGFSNVHFMFANA
Sbjct: 121 LPLMVDIGCGSGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNVHFMFANA 180

Query: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240
           TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD
Sbjct: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240

Query: 241 VLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGSNIY 300
           VL+VA DMRL FDSKA+VLKHIDKLD+S LCD+DGWLL NPMGIRTEREIHAESEG+NIY
Sbjct: 241 VLQVAVDMRLLFDSKADVLKHIDKLDQSLLCDEDGWLLNNPMGIRTEREIHAESEGANIY 300

Query: 301 RRMYQKY 308
           RRMYQKY
Sbjct: 301 RRMYQKY 307

BLAST of Clc05G03250 vs. NCBI nr
Match: XP_023527130.1 (uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 559.7 bits (1441), Expect = 1.6e-155
Identity = 274/310 (88.39%), Postives = 291/310 (93.87%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAKA---AAPSMVATANKSLCPFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LRCIAAAKA   AAPS VATAN++L   LKRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAVAAPSSVATANRTLSLLLKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLS+KIFE++GHVR+RQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMF 180
           DP LPLMVDIGSGSGRFLLWLAK+HLDSRNFLGLEIRQKLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGS 300
           QSDVLEVA DMRLWFDS  +VLKH+D LD+S LCDKDGWLLKNPMGIRTEREIHAESEG+
Sbjct: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Clc05G03250 vs. NCBI nr
Match: KAG6582546.1 (hypothetical protein SDJN03_22548, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 558.5 bits (1438), Expect = 3.5e-155
Identity = 274/310 (88.39%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAK---AAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LRCIAAAK   AAAPS VATAN++L   +KRPFL +FCLPS SSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSDSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLS+KIFE++GHVR+RQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMF 180
           DPKLPLMVDIGSGSGRFLLWLAK+HLDSRNFLGLEIRQKLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPKLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGS 300
           QSDVLEVA DMRLWFDS  NVLKH D LD+S LCDKDGWLLKNPMGIRTEREIHAESEG+
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHADVLDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Clc05G03250 vs. NCBI nr
Match: XP_022924717.1 (uncharacterized protein LOC111432133 [Cucurbita moschata])

HSP 1 Score: 557.8 bits (1436), Expect = 6.0e-155
Identity = 273/310 (88.06%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAK---AAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LRCIAAAK   AAAPS VATAN++L   +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLS+KIFE++GHVR+RQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMF 180
           DP LPLMVDIGSGSGRFLLWLAK+HLDSRNFLGLEIRQKLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGS 300
           QSDVLEVA DMRLWFDS  NVLKH+D  D+S LCDKDGWLLKNPMGIRTEREIHAESEG+
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Clc05G03250 vs. NCBI nr
Match: KAG7018929.1 (trmB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 556.6 bits (1433), Expect = 1.3e-154
Identity = 271/310 (87.42%), Postives = 291/310 (93.87%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAK---AAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LRCIAAAK   AAAPS VATAN++L   +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLS+KIFE++GHVR+RQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMF 180
           DP LPLMVDIGSGSGRFLLWLAK+HLDSRNFLGLEIRQKLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGS 300
           QSDVLEVA DMRLWFDS  +VLKH+D LD+S LCDK+GWLLKNPMGIRTERE+HAESEG+
Sbjct: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Clc05G03250 vs. ExPASy Swiss-Prot
Match: Q2JJQ0 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=trmB PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.1e-45
Identity = 103/214 (48.13%), Postives = 131/214 (61.21%), Query Frame = 0

Query: 88  GHVRVRQHVNPLSSSFSVPTPAPNWDEVFKDPKLPLMVDIGSGSGRFLLWLAKRHLDSRN 147
           GH RVRQHVNPL + +  P P P+W+ V++    P  +DIG+GSGRFLL +A+   D  N
Sbjct: 35  GH-RVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRFLLRIAQEQPD-WN 94

Query: 148 FLGLEIRQKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHF 207
           FLG+EIRQ LV+RA+ W  ELG  NVHF+FAN  +S + + +  PG L  V+IL PDP F
Sbjct: 95  FLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTILFPDPWF 154

Query: 208 KKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLEVADDMRLWFDSKANVLKHIDKLDR 267
           KKRHHKRR++Q  LV  +   L PG  VF+QSD+ EVA++M       A  L+H      
Sbjct: 155 KKRHHKRRIVQPQLVADLALLLRPGSPVFLQSDIQEVAEEM------AARFLEH-----P 214

Query: 268 SFLCDKDGWLLKNPMGIRTEREIHAESEGSNIYR 302
            F     G L  NP+GI TERE         IYR
Sbjct: 215 QFWDPHQGPLDSNPLGIPTEREWQCLQLDLPIYR 233

BLAST of Clc05G03250 vs. ExPASy Swiss-Prot
Match: Q2JRH1 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=trmB PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 9.0e-45
Identity = 103/211 (48.82%), Postives = 130/211 (61.61%), Query Frame = 0

Query: 91  RVRQHVNPLSSSFSVPTPAPNWDEVFKDPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLG 150
           R+RQHVNPL + +  PTP P+W+ V++    PL +DIGSGSGRFLL +A+   D  NFLG
Sbjct: 34  RLRQHVNPLQAQYQQPTPPPHWERVYRRLGQPLHLDIGSGSGRFLLRMAQEQPD-WNFLG 93

Query: 151 LEIRQKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKR 210
           +EIRQ LV+RA+ W  ELG  NVHF+FAN  +S + + +  PG L  V+I  PDP FKKR
Sbjct: 94  VEIRQPLVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTIQFPDPWFKKR 153

Query: 211 HHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLEVADDMRLWFDSKANVLKHIDKLDRSFL 270
           HHKRR++Q  LV  +   L PG  VF+QSDV EVA++M   F      L+H       F 
Sbjct: 154 HHKRRIVQPRLVADLALLLQPGSPVFLQSDVREVAEEMVDRF------LEH-----PQFW 213

Query: 271 CDKDGWLLKNPMGIRTEREIHAESEGSNIYR 302
               G +  NP GI TERE      G  IYR
Sbjct: 214 NPYQGPIDDNPFGIPTEREWQCLQLGLPIYR 230

BLAST of Clc05G03250 vs. ExPASy Swiss-Prot
Match: P73161 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=trmB PE=3 SV=2)

HSP 1 Score: 169.1 bits (427), Expect = 7.9e-41
Identity = 94/217 (43.32%), Postives = 128/217 (58.99%), Query Frame = 0

Query: 90  VRVRQHVNPLSSSFSVPTPAPNWDEVFKDPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFL 149
           VR+RQHVNPLS  +      P+W  V+ D + PL +DIG   GRF L +A++H D  NFL
Sbjct: 4   VRIRQHVNPLSEKYRQVLACPDWATVYDDVQRPLHLDIGCARGRFPLKMAQQHPD-WNFL 63

Query: 150 GLEIRQKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKK 209
           G+EIRQ LV  A+     LG  N+H++F N  +  ++  S++P  L  VSI  PDP FK+
Sbjct: 64  GVEIRQPLVLEANETGDRLGLKNLHYLFGNINVEPEKFFSAFPPTLQRVSIQFPDPWFKQ 123

Query: 210 RHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLEVADDMRLWFDSKANVLKHIDKLDRSF 269
           RH+KRRV Q  LV +I ++L PGG+V +QSDV  VA DMR  F    N +   D      
Sbjct: 124 RHNKRRVAQPELVTAIANALPPGGEVLLQSDVEPVAQDMRDRFAENDNFVFTHD------ 183

Query: 270 LCDKDGWLLKNPMGIRTEREIHAESEGSNIYRRMYQK 307
                 WL +NP+G+ TEREI   +    +YR + Q+
Sbjct: 184 ----TPWLAENPLGVPTEREIACFNLQRPVYRCLLQR 209

BLAST of Clc05G03250 vs. ExPASy Swiss-Prot
Match: P72546 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=trmB PE=3 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 9.6e-39
Identity = 92/216 (42.59%), Postives = 131/216 (60.65%), Query Frame = 0

Query: 90  VRVRQHVNPLSSSFSVPTPAPNWDEVFKDPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFL 149
           VRVRQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 150 GLEIRQKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 209
           GLEIR+ LV  A+   +E   +N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 210 KRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLEVADDMRLWFDSKANVLKHIDKLDRS 269
           KRH KRRV+Q  LV+++  +L  G +VF+QSDVLEV  +M   F ++        +  R+
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAE-------PRFQRT 183

Query: 270 FLCDKDGWLLKNPMGIRTEREIHAESEGSNIYRRMY 305
            L     WL +NP+ + TEREI  +++   +YR ++
Sbjct: 184 CL----DWLPENPLPVPTEREIAVQNKQLPVYRALF 207

BLAST of Clc05G03250 vs. ExPASy Swiss-Prot
Match: Q5N2X5 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=trmB PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 9.6e-39
Identity = 92/216 (42.59%), Postives = 131/216 (60.65%), Query Frame = 0

Query: 90  VRVRQHVNPLSSSFSVPTPAPNWDEVFKDPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFL 149
           VRVRQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 150 GLEIRQKLVKRADIWVKELGFSNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 209
           GLEIR+ LV  A+   +E   +N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 210 KRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLEVADDMRLWFDSKANVLKHIDKLDRS 269
           KRH KRRV+Q  LV+++  +L  G +VF+QSDVLEV  +M   F ++        +  R+
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAE-------PRFQRT 183

Query: 270 FLCDKDGWLLKNPMGIRTEREIHAESEGSNIYRRMY 305
            L     WL +NP+ + TEREI  +++   +YR ++
Sbjct: 184 CL----DWLPENPLPVPTEREIAVQNKQLPVYRALF 207

BLAST of Clc05G03250 vs. ExPASy TrEMBL
Match: A0A6J1E9S1 (uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC111432133 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.9e-155
Identity = 273/310 (88.06%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAK---AAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LRCIAAAK   AAAPS VATAN++L   +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLS+KIFE++GHVR+RQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMF 180
           DP LPLMVDIGSGSGRFLLWLAK+HLDSRNFLGLEIRQKLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGS 300
           QSDVLEVA DMRLWFDS  NVLKH+D  D+S LCDKDGWLLKNPMGIRTEREIHAESEG+
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Clc05G03250 vs. ExPASy TrEMBL
Match: A0A6J1ITE1 (uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.1e-154
Identity = 272/310 (87.74%), Postives = 289/310 (93.23%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAK---AAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSAD 60
           MASSLC  L TCS LRCIAAAK   AAAPS VATAN++L   +KRPFL +FCLPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLHSFCLPSGSSAE 60

Query: 61  LCERQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRSPELVALEYADLNLS+KIFE++GHVR+RQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMF 180
           DP LPLMVDIGSGSGRFLLWLAK+HLDSRNFLGLEIRQKLVKRAD+WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSL PGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQVFI 240

Query: 241 QSDVLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGS 300
           QSDVLEVA DMRLWFDS  NVLKH+D LD+S LCDKDGWLL NPMGIRTEREIHAESEG+
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKY 308
           NIYRRMYQKY
Sbjct: 301 NIYRRMYQKY 310

BLAST of Clc05G03250 vs. ExPASy TrEMBL
Match: A0A5A7U1F8 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003710 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.6e-153
Identity = 270/306 (88.24%), Postives = 286/306 (93.46%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAKAAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSADLCE 60
           MASSLCLHLQTCSHLRC+ AAK AAPS+VATANK L    KRPFL NF LPSGSSADLCE
Sbjct: 1   MASSLCLHLQTCSHLRCLGAAKPAAPSVVATANKILS---KRPFLRNFSLPSGSSADLCE 60

Query: 61  RQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFKDPK 120
           RQPLRS ELVALEYADLNLS+K+ E+ GHVR+RQHVNPLSSSFSVPTPAPNWDEVF+DP 
Sbjct: 61  RQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPT 120

Query: 121 LPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMFANA 180
           LPLMVDIGSGSGRFLLWLAKRHL+SRNFLGLEIRQKLVKRA  WVKELGFSNVHFMFANA
Sbjct: 121 LPLMVDIGSGSGRFLLWLAKRHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMFANA 180

Query: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240
           TISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD
Sbjct: 181 TISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240

Query: 241 VLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGSNIY 300
           VLEVA DMRLWFDS+ ++L+HIDK+D+S LCDKDGWLL NPMGIRTEREIHAESEG+NIY
Sbjct: 241 VLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGANIY 300

Query: 301 RRMYQK 307
           RRMY K
Sbjct: 301 RRMYSK 303

BLAST of Clc05G03250 vs. ExPASy TrEMBL
Match: A0A1S3AVT2 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.7e-153
Identity = 270/306 (88.24%), Postives = 286/306 (93.46%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAKAAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSADLCE 60
           MASSLCLHLQTCSHLRC+AAAK AAPS+VATANK L    KRPFL NF LPSGSSADLCE
Sbjct: 1   MASSLCLHLQTCSHLRCLAAAKPAAPSVVATANKILS---KRPFLRNFSLPSGSSADLCE 60

Query: 61  RQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFKDPK 120
           RQPLRS ELVALEYADLNLS+K+ E+ GHVR+RQHVNPLSSSFSVPTPAPNWDEVF+DP 
Sbjct: 61  RQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPT 120

Query: 121 LPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMFANA 180
           LPLMVDIGSGSGRFLLWLAK HL+SRNFLGLEIRQKLVKRA  WVKELGFSNVHFMFANA
Sbjct: 121 LPLMVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMFANA 180

Query: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240
           TISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD
Sbjct: 181 TISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240

Query: 241 VLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGSNIY 300
           VLEVA DMRLWFDS+ ++L+HIDK+D+S LCDKDGWLL NPMGIRTEREIHAESEG+NIY
Sbjct: 241 VLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGANIY 300

Query: 301 RRMYQK 307
           RRMY K
Sbjct: 301 RRMYSK 303

BLAST of Clc05G03250 vs. ExPASy TrEMBL
Match: A0A0A0L947 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126850 PE=4 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.3e-152
Identity = 271/306 (88.56%), Postives = 286/306 (93.46%), Query Frame = 0

Query: 1   MASSLCLHLQTCSHLRCIAAAKAAAPSMVATANKSLCPFLKRPFLPNFCLPSGSSADLCE 60
           MASSLCLHLQ  SHLRCIAAAK AAPSMVATANK L    KRPFL NF LPSGSSADLCE
Sbjct: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILS---KRPFLRNFSLPSGSSADLCE 60

Query: 61  RQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPTPAPNWDEVFKDPK 120
           RQPLRS ELVALEYADLNLS+K+ E+ GHVR+RQHVNPLSSSFSVPTPAPNWDEVF+DP 
Sbjct: 61  RQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPT 120

Query: 121 LPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMFANA 180
           LPLMVDIGSGSGRFLLWLAKRH +SRNFLGLEIRQKLVKRA IWVKELGFSNVHFMFANA
Sbjct: 121 LPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNVHFMFANA 180

Query: 181 TISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240
           TISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD
Sbjct: 181 TISFKQIISSYPGPLILVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSD 240

Query: 241 VLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTEREIHAESEGSNIY 300
           VLEVA DMRLWFDS+A++L+HI+K+D+S LCDKDGWLL NPMGIRTEREIHAESEG+NIY
Sbjct: 241 VLEVAADMRLWFDSEAHILEHINKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGANIY 300

Query: 301 RRMYQK 307
           RRMY K
Sbjct: 301 RRMYSK 303

BLAST of Clc05G03250 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 371.7 bits (953), Expect = 5.7e-103
Identity = 192/319 (60.19%), Postives = 236/319 (73.98%), Query Frame = 0

Query: 3   SSLCLHLQTCSHLRCIAAAKAAAPSMVATANKSLCP----FLKRPFLPNFC--------- 62
           +SLC   Q CS       + +A P ++A    SL      F++   +P  C         
Sbjct: 2   ASLC---QACS-------SSSATPLLLAKRVFSLASSPFIFMREHKIPRHCNSFRAFNAV 61

Query: 63  -LPSGSSADLC-ERQPLRSPELVALEYADLNLSYKIFEEAGHVRVRQHVNPLSSSFSVPT 122
              S +S  +C + + LRS ELV LEYA+LNL++KI EE GHVR+RQHVNPLSSSFS P 
Sbjct: 62  SASSSASTSVCVDFKDLRSNELVELEYAELNLNHKISEEVGHVRIRQHVNPLSSSFSKPA 121

Query: 123 PAPNWDEVFKDPKLPLMVDIGSGSGRFLLWLAKRHLDSRNFLGLEIRQKLVKRADIWVKE 182
           P P WDEV+KDP LPLMVDIGSGSGRFLLW A ++++SRN+LGLEIRQKLVKRA+ WV E
Sbjct: 122 PVPVWDEVYKDPSLPLMVDIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNE 181

Query: 183 LGFSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVESIVD 242
           LG SNVHF+FANA +SF+ +ISSYPGPL +VSILCPDPHFKKRH KRRV+QKPLV SI+ 
Sbjct: 182 LGLSNVHFIFANAMVSFEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQ 241

Query: 243 SLMPGGQVFIQSDVLEVADDMRLWFDSKANVLKHIDKLDRSFLCDKDGWLLKNPMGIRTE 302
           +L PGG++F+QSDVL+VA DMR   D ++NVL+H+D +D      +DGWL +NPMGIRTE
Sbjct: 242 NLKPGGKIFVQSDVLDVAQDMRDQLDEESNVLQHMDTVD-----TEDGWLTENPMGIRTE 301

Query: 303 REIHAESEGSNIYRRMYQK 307
           REIHAE EG+ IYRR+YQK
Sbjct: 302 REIHAEFEGARIYRRLYQK 305

BLAST of Clc05G03250 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-11
Identity = 52/175 (29.71%), Postives = 83/175 (47.43%), Query Frame = 0

Query: 91  RVRQHVNPLSSS-FSVPTPAPNWDEVFKDPKL----------PLMVDIGSGSGRFLLWLA 150
           R R H NPLS S F +P    + D     PK               DIG G G  L+ LA
Sbjct: 23  RARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKFIKKVEFADIGCGFGGLLISLA 82

Query: 151 KRHLDSRNFLGLEIRQKLVKRADIWVKELGFSNVHFMFANATI----SFKQIISSY-PGP 210
               D+   +G+E+R K+ +     +  L  ++    + N ++    S K I + +  G 
Sbjct: 83  TLFPDTL-MIGMELRDKVTEYVKERILALRRTSSEGQYENISVVRTNSMKYIPNYFEKGQ 142

Query: 211 LMLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQSDVLEVADDMR 250
           L  +  L PDPHFK+++H+RRV+   L++     L  GG ++  +DV E+ + M+
Sbjct: 143 LSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTITDVEELGEWMK 196

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879912.15.1e-16292.83tRNA (guanine-N(7)-)-methyltransferase [Benincasa hispida][more]
XP_023527130.11.6e-15588.39uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo][more]
KAG6582546.13.5e-15588.39hypothetical protein SDJN03_22548, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022924717.16.0e-15588.06uncharacterized protein LOC111432133 [Cucurbita moschata][more]
KAG7018929.11.3e-15487.42trmB [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q2JJQ03.1e-4548.13tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-... [more]
Q2JRH19.0e-4548.82tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX... [more]
P731617.9e-4143.32tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / K... [more]
P725469.6e-3942.59tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 79... [more]
Q5N2X59.6e-3942.59tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 /... [more]
Match NameE-valueIdentityDescription
A0A6J1E9S12.9e-15588.06uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC1114321... [more]
A0A6J1ITE11.1e-15487.74uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752... [more]
A0A5A7U1F81.6e-15388.24tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3AVT22.7e-15388.24tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 P... [more]
A0A0A0L9472.3e-15288.56Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126850 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G17660.15.7e-10360.19tRNA (guanine-N-7) methyltransferase [more]
AT5G24840.11.8e-1129.71tRNA (guanine-N-7) methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePFAMPF02390Methyltransf_4coord: 122..301
e-value: 5.8E-33
score: 113.8
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePANTHERPTHR234173-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASEcoord: 53..306
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePROSITEPS51625SAM_MT_TRMBcoord: 80..306
score: 52.687393
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 91..308
e-value: 4.4E-54
score: 184.8
NoneNo IPR availablePANTHERPTHR23417:SF21TRNA (GUANINE-N(7)-)-METHYLTRANSFERASEcoord: 53..306
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 125..238
e-value: 2.23848E-4
score: 38.1799
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 119..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc05G03250.1Clc05G03250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0106004 tRNA (guanine-N7)-methylation
biological_process GO:0006400 tRNA modification
cellular_component GO:0043527 tRNA methyltransferase complex
molecular_function GO:0008176 tRNA (guanine-N7-)-methyltransferase activity