Clc05G02730 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc05G02730
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	Abhydrolase_3 domain-containing protein
Location	ClcChr05: 1835460 .. 1836405 (-)
RNA-Seq Expression	Clc05G02730
Synteny	Clc05G02730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAATCCCCCTCTGCAACTGCAACAACCTCCATTAGTGTTCCCTTCGAGCTTCTCAACATCAAGCTCAACCCAGACGGCTCAATCACCAGACCTGACCTATTTCCCACAATATCCCCGACACAAACCCCCAATCCCAACACCTATAATGTCTCTACAATTTCTAAAGACATTCCCCTAAACCCTTCCAATAAAACCTTCATTCGCCTCTATAAGCCCACAATATTCCCCAAAATCCCACCAACAAACTTCCCATTTTGATCTACGCCCATGGCGGCGGCTTCATTTTCTTCAGCGCAGCCACACAGCCATTTCACGACTTGTGTTGTCGTCTTTCTGCTCATCTTCCAGCCCTCGTCGTGTCCTTAGAGTACCGCCTCACCCCTGAACATCGTCTACGGGCAGCCTACGACAATGCCTTTCAAGCCCTCGGATGGCTTAGCCACGCTGATTTAATCGACGGTGGCGATTGCGAGCCATGGTTGAAAGAATGTGCCGACTTTTCGCGGTGTTTTTTTTCGGCAGTAGCTCAGGCGGGAGCATTATGTTCTATGCAGCTTTACGAGCACTGGATTCAGATCTTACGCCGGTTAAAATCGTGGGGCATATATTTAATCAGCCGTTTTTCGGTGGCACGAAACGGATGGAATCGGAGCTCCGGTGGGCGAACGACCGCATTTAATAAAGCATTTTGTCACACCAAGAGAAGATCGGACGGCTGGAAAGGTGCTTGGTGAGAGGGCATGGTGGGGATCCGTTGATTGATAGGCAGAAGGAGTTTGTGAAGCTACTAGAGGCACGTGGGGTTCACGTGGATGCCAGATTTCACGAGGGAGGATTTCATGGCGTGGAGCTATTTGAACCGTCCAAACTCCAAATTCTGGAGGATGATATAAAGGATTTCATCGATGCTTCCACGGAGGGATCCTCATTGTTGCAGTGA

mRNA sequence

ATGGCGGAATCCCCCTCTGCAACTGCAACAACCTCCATTAGTGTTCCCTTCGAGCTTCTCAACATCAAGCTCAACCCAGACGGCTCAATCACCAGACCTGACCTATTTCCCACAATATCCCCGACACAAACCCCCAATCCCAACACCTATAATCCATTTCACGACTTGTGTTGTCGTCTTTCTGCTCATCTTCCAGCCCTCGTCGTGTCCTTAGAGTACCGCCTCACCCCTGAACATCGTCTACGGGCAGCCTACGACAATGCCTTTCAAGCCCTCGGATGGCTTAGCCACGCTGATTTAATCGACGGTGGCGATTGCGAGCCATGGTTGAAAGAATGTGCCGACTTTTCGCGGTGTTTTTTTTCGGCAGTAGCTCAGGCGGGAGCATTATGTTCTATGCAGCTTTACGAGCACTGGATTCAGATCTTACGCCGGTTAAAATCGTGGGGCATATATTTAATCAGCCGTTTTTCGGTGGCACGAAACGGATGGAATCGGAGCTCCGGTGGGCGAACGACCGCATTTAATAAAGCATTTTGTCACACCAAGAGAAGATCGGACGGCTGGAAAGGTGCTTGGCAGAAGGAGTTTGTGAAGCTACTAGAGGCACGTGGGGTTCACGTGGATGCCAGATTTCACGAGGGAGGATTTCATGGCGTGGAGCTATTTGAACCGTCCAAACTCCAAATTCTGGAGGATGATATAAAGGATTTCATCGATGCTTCCACGGAGGGATCCTCATTGTTGCAGTGA

Coding sequence (CDS)

ATGGCGGAATCCCCCTCTGCAACTGCAACAACCTCCATTAGTGTTCCCTTCGAGCTTCTCAACATCAAGCTCAACCCAGACGGCTCAATCACCAGACCTGACCTATTTCCCACAATATCCCCGACACAAACCCCCAATCCCAACACCTATAATCCATTTCACGACTTGTGTTGTCGTCTTTCTGCTCATCTTCCAGCCCTCGTCGTGTCCTTAGAGTACCGCCTCACCCCTGAACATCGTCTACGGGCAGCCTACGACAATGCCTTTCAAGCCCTCGGATGGCTTAGCCACGCTGATTTAATCGACGGTGGCGATTGCGAGCCATGGTTGAAAGAATGTGCCGACTTTTCGCGGTGTTTTTTTTCGGCAGTAGCTCAGGCGGGAGCATTATGTTCTATGCAGCTTTACGAGCACTGGATTCAGATCTTACGCCGGTTAAAATCGTGGGGCATATATTTAATCAGCCGTTTTTCGGTGGCACGAAACGGATGGAATCGGAGCTCCGGTGGGCGAACGACCGCATTTAATAAAGCATTTTGTCACACCAAGAGAAGATCGGACGGCTGGAAAGGTGCTTGGCAGAAGGAGTTTGTGAAGCTACTAGAGGCACGTGGGGTTCACGTGGATGCCAGATTTCACGAGGGAGGATTTCATGGCGTGGAGCTATTTGAACCGTCCAAACTCCAAATTCTGGAGGATGATATAAAGGATTTCATCGATGCTTCCACGGAGGGATCCTCATTGTTGCAGTGA

Protein sequence

MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTPNPNTYNPFHDLCCRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWLSHADLIDGGDCEPWLKECADFSRCFFSAVAQAGALCSMQLYEHWIQILRRLKSWGIYLISRFSVARNGWNRSSGGRTTAFNKAFCHTKRRSDGWKGAWQKEFVKLLEARGVHVDARFHEGGFHGVELFEPSKLQILEDDIKDFIDASTEGSSLLQ

Homology

[Close]

BLAST of Clc05G02730 vs. NCBI nr
Match: XP_023526606.1 (probable carboxylesterase 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 221.1 bits (562), Expect = 1.1e-53
Identity = 149/348 (42.82%), Postives = 173/348 (49.71%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTPNPNTYN--------- 60
           MAES SATATTSI  PFE LNIKLNPDGSITR + FPT+ PT+TP PNT N         
Sbjct: 1   MAESLSATATTSIIDPFEHLNIKLNPDGSITRHNQFPTVPPTETPTPNTNNLSTCCSKDI 60

Query: 61  ---------------------------------------------PFHDLCCRLSAHLPA 120
                                                         FHD CCRLSAHLPA
Sbjct: 61  PLNPSNNTFIRLFKPHNIPQNPTNKLPILIDAHGGGFILFSAATQLFHDTCCRLSAHLPA 120

Query: 121 LVVSLEYRLTPEHRLRAAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSRCFFSAVA 180
           L+VS EYRL PEHRL AAYD+A +AL WL   A+ I GGDC+PWLK+CADFSRCF    +
Sbjct: 121 LIVSFEYRLAPEHRLPAAYDDALEALRWLCRQANQIHGGDCDPWLKDCADFSRCFLLGTS 180

Query: 181 QAGALCSMQLYEHWIQILRRLKSWGIYLISRFSVARNGWNRS-SGGRTTAFNKAFCHTKR 240
             G +        +   LR L S     +S   +  + +N+   GG     ++  C   R
Sbjct: 181 AGGNIT-------FYAALRALDS----DLSPVKIVGHIYNQPFFGGIKRTESELRCVNDR 240

Query: 241 -------------------RSDGW------KGAW-----------------------QKE 245
                                D W      +G                         QKE
Sbjct: 241 ILPLSVNDLMWSLALPEGCDRDHWFCDPTVEGPHQEKIRRLQRCLVRGHVGDPLIDRQKE 300

[Close]

BLAST of Clc05G02730 vs. NCBI nr
Match: KAG6582576.1 (putative carboxylesterase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 215.3 bits (547), Expect = 5.9e-52
Identity = 145/314 (46.18%), Postives = 168/314 (53.50%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTP-NPNTYNP------- 60
           MAES SATATTSI  PFE LNIKLNPDGSITR + FPT  P   P NP    P       
Sbjct: 1   MAESLSATATTSIIDPFEHLNIKLNPDGSITRHNQFPT--PHNIPQNPTNKLPILIDAHG 60

Query: 61  ------------FHDLCCRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWL-SHAD 120
                       FH+ CCRLSAHLPAL+VS EYRL PEHRL AAYD+A +AL WL   A+
Sbjct: 61  GGFILFSAATQLFHETCCRLSAHLPALIVSFEYRLAPEHRLPAAYDDALEALRWLCRQAN 120

Query: 121 LIDGGDCEPWLKECADFSRCFFSAVAQAGALCSMQLYEHWIQILRRLKSWGIYLISRFSV 180
            I GGDC+PWLK+CADFSRCF    +  G +        +   LR L S     +S   +
Sbjct: 121 QIHGGDCDPWLKDCADFSRCFLLGTSAGGNIT-------FYAALRALDS----DLSPVKI 180

Query: 181 ARNGWNRS-SGGRTTAFNKAFCHTKR-------------------RSDGW------KGAW 240
             + +N+   GG     ++  C   R                     D W      +G  
Sbjct: 181 VGHIYNQPFFGGIKRTESELRCVNDRILPLSVNDLMWSLALPEGCDRDHWFCDPTVEGPH 240

Query: 241 -----------------------QKEFVKLLEARGVHVDARFHEGGFHGVELFEPSKLQI 245
                                  QKEFVKLLEARGV VDARF EGG+HGV+LF+PSKLQI
Sbjct: 241 QEKIRRLQRCLVRGHVGDPLIDRQKEFVKLLEARGVKVDARFREGGYHGVDLFDPSKLQI 300

[Close]

BLAST of Clc05G02730 vs. NCBI nr
Match: XP_022924288.1 (probable carboxylesterase 8 [Cucurbita moschata])

HSP 1 Score: 215.3 bits (547), Expect = 5.9e-52
Identity = 146/348 (41.95%), Postives = 171/348 (49.14%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTPNPNTYN--------- 60
           MAES SATAT SI  PFE LNIKLNPDGSITR +  PT+ PT+TP PNT N         
Sbjct: 1   MAESLSATATASIIDPFEHLNIKLNPDGSITRHNQLPTVPPTETPTPNTNNLSTCCSKDI 60

Query: 61  ---------------------------------------------PFHDLCCRLSAHLPA 120
                                                         FH+ CCRLSAHLPA
Sbjct: 61  PLNPSNNTFIRLFKPHNIPQNPTNKLPILIDAHGGGFILFSAATQLFHETCCRLSAHLPA 120

Query: 121 LVVSLEYRLTPEHRLRAAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSRCFFSAVA 180
           L+VS EYRL PEHRL AAYD+A +AL WL   A+ I GGDC+PWLK+CADFSRCF    +
Sbjct: 121 LIVSFEYRLAPEHRLPAAYDDALEALRWLCRQANQIHGGDCDPWLKDCADFSRCFLLGTS 180

Query: 181 QAGALCSMQLYEHWIQILRRLKSWGIYLISRFSVARNGWNRS-SGGRTTAFNKAFCHTKR 240
             G +        +   LR L S     +S   +  + +N+   GG     ++  C   R
Sbjct: 181 AGGNIT-------FYAALRALDS----DLSPVKIVGHIYNQPFFGGIKRTESELRCVNDR 240

Query: 241 -------------------RSDGW------KGAW-----------------------QKE 245
                                D W      +G                         QKE
Sbjct: 241 ILPLSVNDLMWSLALPEGCDRDHWFCDPTVEGPHQEKIRRLQRCLVRGHVGDPLIDRQKE 300

[Close]

BLAST of Clc05G02730 vs. NCBI nr
Match: PQQ17285.1 (putative carboxylesterase 8 [Prunus yedoensis var. nudiflora])

HSP 1 Score: 143.3 bits (360), Expect = 2.9e-30
Identity = 106/315 (33.65%), Postives = 141/315 (44.76%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTPN-------------- 60
           MA+ PS T T++   P++LLNI  NPDGS+TR   FP + P+ TP               
Sbjct: 1   MADKPSQTPTSN-DDPYKLLNIVKNPDGSLTRHAPFPMVPPSPTPTDSISSTAAATELFK 60

Query: 61  -----PNT-------------------YNPFHDLCCRLSAHLPALVVSLEYRLTPEHRLR 120
                PN+                     PFHD C RL+  LPALVV++EYRL PEH L 
Sbjct: 61  PHPLPPNSKLPLIFYFHGGGFILFSVALQPFHDSCSRLALSLPALVVAVEYRLAPEHPLP 120

Query: 121 AAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSRCF--------------------- 180
           +AYD+A +A+ W  S A  I G  C+PWLK+  DFS+CF                     
Sbjct: 121 SAYDDAIEAVNWARSQASNIYG--CDPWLKDTVDFSKCFLLGSSAGANIVYHAGLRIAGV 180

Query: 181 -FSAVAQAGALCSMQLY--------------EHWIQILRRLKSWGIYLISRFSVARNGWN 240
             S V   G + +   +              +  + ++     W + L           N
Sbjct: 181 DLSPVKIRGLIFNQPYFGGVERTQSELRLINDRILPLVSNDLMWALSLPKGADRNHEYSN 240

[Close]

BLAST of Clc05G02730 vs. NCBI nr
Match: CAB4300203.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-29
Identity = 115/339 (33.92%), Postives = 147/339 (43.36%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTP--------------- 60
           MA+ PS T T++   P++LLNI  NPDGS+TR   FP + P+ TP               
Sbjct: 1   MADKPSQTPTSN-DDPYKLLNIVKNPDGSLTRHPPFPMVPPSPTPTDSISSTAAATESNS 60

Query: 61  -----------NPNTYN-----------------------------------PFHDLCCR 120
                      NP T N                                   PFHD C R
Sbjct: 61  PQLVLSKDIQINPTTKNSVRLFKPHPLPPNSKLPLIFYFHGGGFILFSVALQPFHDSCSR 120

Query: 121 LSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSR 180
           ++  LPALVVS+EYRL PEH L +AYD+A +A+ W  S A  I+G  C+PWLK+  DFS+
Sbjct: 121 MALSLPALVVSVEYRLAPEHPLPSAYDDAVEAVHWARSQASNING--CDPWLKDTVDFSK 180

Query: 181 CFFSAVAQAGA---------LCSMQLYEHWIQIL----------RRLKS----------- 240
           CF    + AGA         +  + L    IQ L           R +S           
Sbjct: 181 CFLMG-SSAGANIVYHAGLRVAGVDLSPVKIQGLIFNQPYFGGVERTQSELRLINDRILP 240

[Close]

BLAST of Clc05G02730 vs. ExPASy Swiss-Prot
Match: O64640 (Probable carboxylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=CXE8 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 6.7e-22
Identity = 94/320 (29.38%), Postives = 138/320 (43.12%), Query Frame = 0


Query: 14  SVPFELLNIKLNPDGSITRPDLFPTISPTQ----TPNPNTYN------------------ 73
           S P++ LNI LN DGS+TR   FP + PT+     P   T N                  
Sbjct: 8   SDPYKFLNITLNSDGSLTRHRDFPKLPPTEQSKDIPLNQTNNTFIRIFKPRNIPPESKLP 67

Query: 74  ------------------PFHDLCCRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALG 133
                             PFH+ C +++  L  +++S+EYRL PEHRL AAY++A +A+ 
Sbjct: 68  ILVYFHGGGFILYSAASAPFHESCTKMADRLQTIILSVEYRLAPEHRLPAAYEDAVEAIL 127

Query: 134 WL--SHADLIDGGDCEPWLKECADFSRCFFSAVAQAG--------ALCSMQLYEHWIQIL 193
           WL       I+GGDC+ WLK+  DFS+C+    +  G         +    L    IQ L
Sbjct: 128 WLRDQARGPINGGDCDTWLKDGVDFSKCYVMGSSSGGNIVYNVALRVVDTDLSPVKIQGL 187

Query: 194 ----------------RRLKS------------WGIYL---ISRFSVARNGWNRSSGGRT 248
                            RLK             W + L   + R  V  N   +SSG + 
Sbjct: 188 IMNQAFFGGVEPSDSESRLKDDKICPLPATHLLWSLCLPDGVDRDHVYSNP-IKSSGPQE 247

[Close]

BLAST of Clc05G02730 vs. ExPASy Swiss-Prot
Match: O64641 (Probable carboxylesterase 9 OS=Arabidopsis thaliana OX=3702 GN=CXE9 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 5.8e-18
Identity = 90/331 (27.19%), Postives = 130/331 (39.27%), Query Frame = 0


Query: 16  PFELLNIKLNPDGSITRPDLFPTISPTQTPNPN-----------------------TYNP 75
           P++ LNI +NP+GS TR  ++P + P   P P                        T  P
Sbjct: 11  PYKHLNITINPNGSCTRHFVWPRVEPDPDPCPGKLAASKDVTINHETGVSVRIFRPTNLP 70

Query: 76  FHD-----------------------------LCCRLSAHLPALVVSLEYRLTPEHRLRA 135
            +D                              C ++++ L  +VVS+ YRL PEHRL A
Sbjct: 71  SNDNAVARLPIIIHLHGSGWILYPANSAANDRCCSQMASELTVIVVSVHYRLPPEHRLPA 130

Query: 136 AYDNAFQALGWLSHADLIDGGDCEPWLKECADFSRCFFSAVAQAGALCSMQL----YEHW 195
            YD+A  AL W+    ++D  + EPWLK+ ADFSRC+    +  GA  + QL     +H 
Sbjct: 131 QYDDALDALLWVKQ-QVVDSTNGEPWLKDYADFSRCYICG-SSNGANIAFQLALRSLDHD 190

Query: 196 IQILR---------------RLKS------------------WGI--------------- 240
           +  L+               R KS                  W +               
Sbjct: 191 LTPLQIDGCVFYQPLFGGKTRTKSELKNFADPVMPVPAVDAMWELSLPVGVDRDHRYCNP 250

[Close]

BLAST of Clc05G02730 vs. ExPASy Swiss-Prot
Match: A0A0A1EQ07 (3-O-acetylpapaveroxine carboxylesterase CXE2 OS=Papaver somniferum OX=3469 GN=CXE2 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-17
Identity = 73/229 (31.88%), Postives = 104/229 (45.41%), Query Frame = 0


Query: 54  HDLCCRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWLSHADLIDGGDCEPWLKEC 113
           HD C  ++ HLPALVVS++YRL PE+RL AAYD+A  AL W+    L    + E WLKE 
Sbjct: 87  HDFCQSIATHLPALVVSVDYRLAPENRLPAAYDDAVDALNWVKDQGLGKLNNSEVWLKEY 146

Query: 114 ADFSRCFFSAVAQAGALC------------------SMQLYEHWIQILRRLKS------- 173
            DFS+CF    +  G +                    + L+  +   L+R +S       
Sbjct: 147 GDFSKCFIMGCSSGGNIAYHASLRAIEMDLEPVIINGLILHSPFFGSLQRTESDLKVIND 206

Query: 174 -----------WGIYL---ISRFSVARNGWNRSSGGRTTAFNKAFCHTKRRSDGWKG--- 233
                      W + L    SR  V  N  N ++ G ++  N A    +    G+ G   
Sbjct: 207 QDLPLAVRDVMWELALPLGSSRDHVYCNP-NIANDGSSSG-NMAGLIKRCLVIGFYGDPL 266

Query: 234 -AWQKEFVKLLEARGVHVDARFHEGGFHGVELFEPSKLQILEDDIKDFI 240
              Q + VK+LE +GV V+    + G+HGV  F+P   + L   IK FI
Sbjct: 267 IDRQIQLVKMLEEKGVKVETWIEQEGYHGVPCFDPKIRETLLGKIKYFI 313

[Close]

BLAST of Clc05G02730 vs. ExPASy Swiss-Prot
Match: I3PLR2 (3-O-acetylpapaveroxine carboxylesterase CXE1 OS=Papaver somniferum OX=3469 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.9e-17
Identity = 85/286 (29.72%), Postives = 115/286 (40.21%), Query Frame = 0


Query: 21  NIKLNPDGS----ITRPDLFPTISPTQTPN-------------------PNTYNPFHDLC 80
           +I LNPD      I RP   PT  P  T N                    +T N  HD C
Sbjct: 36  DISLNPDRKTSLRIFRP---PTKEPPVTKNKLLPIIIYFHGGGFILFNADSTMN--HDFC 95

Query: 81  CRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWLSHADLIDGGDCEPWLKECADFS 140
             ++ H+PALVVS++YRL PE+RL AAYD+A  AL W+    L    + E WLKE  DFS
Sbjct: 96  QSIATHIPALVVSVDYRLAPENRLPAAYDDAVDALNWVKDQGLGKLNNSEVWLKEYGDFS 155

Query: 141 RCFF---SAVAQAGALCSMQLYEHWIQILRRLKSWGIYLISRF----------SVARNGW 200
           +CF    S+ A      S++  E     L   K  G+ L   F          S   N  
Sbjct: 156 KCFIMGCSSGANVAYHASLRAIE---MDLEPAKINGLILHCPFFGSLERTESDSKVINNQ 215

Query: 201 NRSSGGRTTAFNKA----------FCHTKRRSDGWKGA---------------------W 240
           +     R   +  A          +C+     DG                          
Sbjct: 216 DLPLAVRDVMWELALPLGSTRDHVYCNPNIDHDGSSSGNMVGLIERCFVVGFYGDPLIDR 275

[Close]

BLAST of Clc05G02730 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.5e-15
Identity = 66/231 (28.57%), Postives = 98/231 (42.42%), Query Frame = 0


Query: 53  FHDLCCRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWLSHADLIDGGDCEPWLKE 112
           FHD CC ++ H   ++ S++YRL PEHRL AAYD+A +AL W+  +        + WL  
Sbjct: 104 FHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKDSR-------DEWLTN 163

Query: 113 CADFSRCFFSAVAQAG----------ALCSMQLYEHWIQIL------------------- 172
            ADFS CF    +  G          A  + +L    I+ L                   
Sbjct: 164 FADFSNCFIMGESAGGNIAYHAGLRAAAVADELLPLKIKGLVLDEPGFGGSKRTGSELRL 223

Query: 173 ---RRLKSWGIYLISRFSVARNGWNRSSGGRTTAFNK-AFCHTKRRSDGWKGA------- 232
               RL ++ + LI   S+             TA ++  +   K RS GW+         
Sbjct: 224 ANDSRLPTFVLDLIWELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGD 283

Query: 233 ----WQKEFVKLLEARGVHVDARFHEGGFHGVELFEPSKLQILEDDIKDFI 240
                Q E  + LE +GV V A+F  GG+H V+L +P K +     +K F+
Sbjct: 284 PMIDRQMELAERLEKKGVDVVAQFDVGGYHAVKLEDPEKAKQFFVILKKFV 327

[Close]

BLAST of Clc05G02730 vs. ExPASy TrEMBL
Match: A0A6J1EC01 (probable carboxylesterase 8 OS=Cucurbita moschata OX=3662 GN=LOC111431817 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 2.9e-52
Identity = 146/348 (41.95%), Postives = 171/348 (49.14%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTPNPNTYN--------- 60
           MAES SATAT SI  PFE LNIKLNPDGSITR +  PT+ PT+TP PNT N         
Sbjct: 1   MAESLSATATASIIDPFEHLNIKLNPDGSITRHNQLPTVPPTETPTPNTNNLSTCCSKDI 60

Query: 61  ---------------------------------------------PFHDLCCRLSAHLPA 120
                                                         FH+ CCRLSAHLPA
Sbjct: 61  PLNPSNNTFIRLFKPHNIPQNPTNKLPILIDAHGGGFILFSAATQLFHETCCRLSAHLPA 120

Query: 121 LVVSLEYRLTPEHRLRAAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSRCFFSAVA 180
           L+VS EYRL PEHRL AAYD+A +AL WL   A+ I GGDC+PWLK+CADFSRCF    +
Sbjct: 121 LIVSFEYRLAPEHRLPAAYDDALEALRWLCRQANQIHGGDCDPWLKDCADFSRCFLLGTS 180

Query: 181 QAGALCSMQLYEHWIQILRRLKSWGIYLISRFSVARNGWNRS-SGGRTTAFNKAFCHTKR 240
             G +        +   LR L S     +S   +  + +N+   GG     ++  C   R
Sbjct: 181 AGGNIT-------FYAALRALDS----DLSPVKIVGHIYNQPFFGGIKRTESELRCVNDR 240

Query: 241 -------------------RSDGW------KGAW-----------------------QKE 245
                                D W      +G                         QKE
Sbjct: 241 ILPLSVNDLMWSLALPEGCDRDHWFCDPTVEGPHQEKIRRLQRCLVRGHVGDPLIDRQKE 300

[Close]

BLAST of Clc05G02730 vs. ExPASy TrEMBL
Match: A0A314ZB62 (Putative carboxylesterase 8 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_39350 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 1.4e-30
Identity = 106/315 (33.65%), Postives = 141/315 (44.76%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTPN-------------- 60
           MA+ PS T T++   P++LLNI  NPDGS+TR   FP + P+ TP               
Sbjct: 1   MADKPSQTPTSN-DDPYKLLNIVKNPDGSLTRHAPFPMVPPSPTPTDSISSTAAATELFK 60

Query: 61  -----PNT-------------------YNPFHDLCCRLSAHLPALVVSLEYRLTPEHRLR 120
                PN+                     PFHD C RL+  LPALVV++EYRL PEH L 
Sbjct: 61  PHPLPPNSKLPLIFYFHGGGFILFSVALQPFHDSCSRLALSLPALVVAVEYRLAPEHPLP 120

Query: 121 AAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSRCF--------------------- 180
           +AYD+A +A+ W  S A  I G  C+PWLK+  DFS+CF                     
Sbjct: 121 SAYDDAIEAVNWARSQASNIYG--CDPWLKDTVDFSKCFLLGSSAGANIVYHAGLRIAGV 180

Query: 181 -FSAVAQAGALCSMQLY--------------EHWIQILRRLKSWGIYLISRFSVARNGWN 240
             S V   G + +   +              +  + ++     W + L           N
Sbjct: 181 DLSPVKIRGLIFNQPYFGGVERTQSELRLINDRILPLVSNDLMWALSLPKGADRNHEYSN 240

[Close]

BLAST of Clc05G02730 vs. ExPASy TrEMBL
Match: A0A6J5WIR3 (Abhydrolase_3 domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS15167 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 5.3e-30
Identity = 115/339 (33.92%), Postives = 147/339 (43.36%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTP--------------- 60
           MA+ PS T T++   P++LLNI  NPDGS+TR   FP + P+ TP               
Sbjct: 1   MADKPSQTPTSN-DDPYKLLNIVKNPDGSLTRHPPFPMVPPSPTPTDSISSTAAATESNS 60

Query: 61  -----------NPNTYN-----------------------------------PFHDLCCR 120
                      NP T N                                   PFHD C R
Sbjct: 61  PQLVLSKDIQINPTTKNSVRLFKPHPLPPNSKLPLIFYFHGGGFILFSVALQPFHDSCSR 120

Query: 121 LSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSR 180
           ++  LPALVVS+EYRL PEH L +AYD+A +A+ W  S A  I+G  C+PWLK+  DFS+
Sbjct: 121 MALSLPALVVSVEYRLAPEHPLPSAYDDAVEAVHWARSQASNING--CDPWLKDTVDFSK 180

Query: 181 CFFSAVAQAGA---------LCSMQLYEHWIQIL----------RRLKS----------- 240
           CF    + AGA         +  + L    IQ L           R +S           
Sbjct: 181 CFLMG-SSAGANIVYHAGLRVAGVDLSPVKIQGLIFNQPYFGGVERTQSELRLINDRILP 240

[Close]

BLAST of Clc05G02730 vs. ExPASy TrEMBL
Match: A0A6P5TG45 (probable carboxylesterase 8 OS=Prunus avium OX=42229 GN=LOC110766902 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 9.0e-30
Identity = 108/340 (31.76%), Postives = 145/340 (42.65%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTP--------------- 60
           MA+ PS T T++   P++LLNI  NPDGS+TR   FP + P+ TP               
Sbjct: 1   MADKPSQTPTSN-DDPYKLLNIVKNPDGSLTRHAPFPIVPPSPTPTDSISSTAAATESNS 60

Query: 61  -----------NPNTYN-----------------------------------PFHDLCCR 120
                      NP T N                                   PFHD C R
Sbjct: 61  PQLVLSKDIQINPTTKNSVRLFKPHPLPPNSKLPLIFYFHGGGFVLFSVALQPFHDSCSR 120

Query: 121 LSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSR 180
           ++  LPALVV++EYRL PEH L +AYD+A +A+ W  S A  I+G  C+PWLK+  DFS+
Sbjct: 121 MALSLPALVVAVEYRLAPEHPLPSAYDDAIEAVNWTRSQASNING--CDPWLKDTVDFSK 180

Query: 181 CF----------------------FSAVAQAGALCSMQLY--------------EHWIQI 240
           CF                       S V   G + +   +              +  + +
Sbjct: 181 CFLLGSSAGANIVYHAGLRIAGVDLSPVKIRGLIFNQPYFGGVERTQSELRLINDRILPL 240

Query: 241 LRRLKSWGIYLISRFSVARNGWNRSSGGRTTAFNK-AFCHTKRRSDGWKGAWQKEFVKLL 242
           +     W + L           N + GG      +   C  +          QKEFVKLL
Sbjct: 241 VSNDLMWALSLPKGADRNHEYSNPTVGGGDERIGRLPTCIVRGFGGDPLVDRQKEFVKLL 300

[Close]

BLAST of Clc05G02730 vs. ExPASy TrEMBL
Match: A0A6J5U0I2 (Abhydrolase_3 domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS15609 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 9.0e-30
Identity = 114/339 (33.63%), Postives = 147/339 (43.36%), Query Frame = 0


Query: 1   MAESPSATATTSISVPFELLNIKLNPDGSITRPDLFPTISPTQTP--------------- 60
           MA+ PS T T++   P++LLNI  NPDGS+TR   FP + P+ TP               
Sbjct: 1   MADKPSQTPTSN-DDPYKLLNIVKNPDGSLTRHPPFPMVPPSPTPTDSISSTAAATESNS 60

Query: 61  -----------NPNTYN-----------------------------------PFHDLCCR 120
                      NP T N                                   PFHD C R
Sbjct: 61  PQLVLSKDIQINPTTKNSVRLFKPHPLPPNSKLPLIFYFHGGGFILFSVALQPFHDSCSR 120

Query: 121 LSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWL-SHADLIDGGDCEPWLKECADFSR 180
           ++  LPALVVS+EYRL PEH L +AYD+A +A+ W  S A  I+G  C+PWLK+  DFS+
Sbjct: 121 MALSLPALVVSVEYRLAPEHPLPSAYDDAVEAVDWARSQASNING--CDPWLKDTVDFSK 180

Query: 181 CFFSAVAQAGA---------LCSMQLYEHWIQIL----------RRLKS----------- 240
           CF    + AGA         +  + L    IQ L           R +S           
Sbjct: 181 CFLMG-SSAGANIVYHAGLRVAGVDLSPVKIQGLIFNQPYFGGVERTQSELRLINDRILP 240

[Close]

BLAST of Clc05G02730 vs. TAIR 10
Match: AT2G45600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 4.7e-23
Identity = 94/320 (29.38%), Postives = 138/320 (43.12%), Query Frame = 0


Query: 14  SVPFELLNIKLNPDGSITRPDLFPTISPTQ----TPNPNTYN------------------ 73
           S P++ LNI LN DGS+TR   FP + PT+     P   T N                  
Sbjct: 8   SDPYKFLNITLNSDGSLTRHRDFPKLPPTEQSKDIPLNQTNNTFIRIFKPRNIPPESKLP 67

Query: 74  ------------------PFHDLCCRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALG 133
                             PFH+ C +++  L  +++S+EYRL PEHRL AAY++A +A+ 
Sbjct: 68  ILVYFHGGGFILYSAASAPFHESCTKMADRLQTIILSVEYRLAPEHRLPAAYEDAVEAIL 127

Query: 134 WL--SHADLIDGGDCEPWLKECADFSRCFFSAVAQAG--------ALCSMQLYEHWIQIL 193
           WL       I+GGDC+ WLK+  DFS+C+    +  G         +    L    IQ L
Sbjct: 128 WLRDQARGPINGGDCDTWLKDGVDFSKCYVMGSSSGGNIVYNVALRVVDTDLSPVKIQGL 187

Query: 194 ----------------RRLKS------------WGIYL---ISRFSVARNGWNRSSGGRT 248
                            RLK             W + L   + R  V  N   +SSG + 
Sbjct: 188 IMNQAFFGGVEPSDSESRLKDDKICPLPATHLLWSLCLPDGVDRDHVYSNP-IKSSGPQE 247

[Close]

BLAST of Clc05G02730 vs. TAIR 10
Match: AT2G45610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 4.1e-19
Identity = 90/331 (27.19%), Postives = 130/331 (39.27%), Query Frame = 0


Query: 16  PFELLNIKLNPDGSITRPDLFPTISPTQTPNPN-----------------------TYNP 75
           P++ LNI +NP+GS TR  ++P + P   P P                        T  P
Sbjct: 11  PYKHLNITINPNGSCTRHFVWPRVEPDPDPCPGKLAASKDVTINHETGVSVRIFRPTNLP 70

Query: 76  FHD-----------------------------LCCRLSAHLPALVVSLEYRLTPEHRLRA 135
            +D                              C ++++ L  +VVS+ YRL PEHRL A
Sbjct: 71  SNDNAVARLPIIIHLHGSGWILYPANSAANDRCCSQMASELTVIVVSVHYRLPPEHRLPA 130

Query: 136 AYDNAFQALGWLSHADLIDGGDCEPWLKECADFSRCFFSAVAQAGALCSMQL----YEHW 195
            YD+A  AL W+    ++D  + EPWLK+ ADFSRC+    +  GA  + QL     +H 
Sbjct: 131 QYDDALDALLWVKQ-QVVDSTNGEPWLKDYADFSRCYICG-SSNGANIAFQLALRSLDHD 190

Query: 196 IQILR---------------RLKS------------------WGI--------------- 240
           +  L+               R KS                  W +               
Sbjct: 191 LTPLQIDGCVFYQPLFGGKTRTKSELKNFADPVMPVPAVDAMWELSLPVGVDRDHRYCNP 250

[Close]

BLAST of Clc05G02730 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 79.0 bits (193), Expect = 6.2e-15
Identity = 86/330 (26.06%), Postives = 115/330 (34.85%), Query Frame = 0


Query: 13  ISVPFELLNIKLNPDGSITRPDLFPTISPTQTPNPNTYNP-------------------- 72
           I+ P+  LNI  NPDGSITR DL        TP+P+  NP                    
Sbjct: 7   IADPYAYLNIVNNPDGSITR-DLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYL 66

Query: 73  -----------------------------------FHDLCCRLSAHLPALVVSLEYRLTP 132
                                              FHD C  ++  L A+VVS  YRL P
Sbjct: 67  PSSAVNEGNVSSQKLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAP 126

Query: 133 EHRLRAAYDNAFQALGWLSHADLIDGGDCEPWLKECADFSRCFFSAVAQAGALC---SMQ 192
           EHRL AAYD+  +AL W+  +D       + W+K  ADFS  F    +  G L     ++
Sbjct: 127 EHRLPAAYDDGVEALDWIKTSD-------DEWIKSHADFSNVFLMGTSAGGNLAYNVGLR 186

Query: 193 LYEHWIQILRRLKSWGIYLISRFSVARNGWNRSSGGRTTAFNKAFC-------------- 240
             +  +  L  L+  G+ L   F     G    S       N   C              
Sbjct: 187 SVDS-VSDLSPLQIRGLILHHPFF----GGEERSESEIRLMNDQVCPPIVTDVMWDLSLP 246

[Close]

BLAST of Clc05G02730 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 64.3 bits (155), Expect = 1.6e-10
Identity = 33/85 (38.82%), Postives = 48/85 (56.47%), Query Frame = 0


Query: 46  NPNTYNPFHDLCCRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWL--SHADLIDG 105
           +PN Y P+ ++C R +  LPA V+S+ YRL PEHR  A YD+ F AL ++  +H  ++  
Sbjct: 102 SPNAY-PYDNVCRRFARKLPAYVISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSILPA 161

Query: 106 GDCEPWLKECADFSRCFFSAVAQAG 129
                     AD SRCFF+  +  G
Sbjct: 162 N---------ADLSRCFFAGDSAGG 176

[Close]

BLAST of Clc05G02730 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 58.5 bits (140), Expect = 8.7e-09
Identity = 44/140 (31.43%), Postives = 63/140 (45.00%), Query Frame = 0


Query: 53  FHDLCCRLSAHLPALVVSLEYRLTPEHRLRAAYDNAFQALGWLSHADLIDGGDCEPWLKE 112
           +HD    L+     ++VS+ YRL PEHRL AAYD+    + WL    +  GG    WL +
Sbjct: 113 YHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK 172

Query: 113 CADFSRCFFSAVAQAGALCSMQLYEHWI---QILRRLKSWGIYLISRFSVARNGWNRSSG 172
           C + S  F  A   AGA  + Q+    +   +    L   GI LI  F     G +R+S 
Sbjct: 173 C-NLSNVFL-AGDSAGANIAYQVAVRIMASGKYANTLHLKGIILIHPFF---GGESRTSS 232

Query: 173 GRTTAFNKAFCHTKRRSDGW 190
            +     K+   T   SD +
Sbjct: 233 EKQQHHTKSSALTLSASDAY 247

The following BLAST results are available for this feature:

BLAST of Clc05G02730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023526606.1	1.1e-53	42.82	probable carboxylesterase 8 [Cucurbita pepo subsp. pepo]	[more]
KAG6582576.1	5.9e-52	46.18	putative carboxylesterase 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022924288.1	5.9e-52	41.95	probable carboxylesterase 8 [Cucurbita moschata]	[more]
PQQ17285.1	2.9e-30	33.65	putative carboxylesterase 8 [Prunus yedoensis var. nudiflora]	[more]
CAB4300203.1	1.1e-29	33.92	unnamed protein product [Prunus armeniaca]	[more]

BLAST of Clc05G02730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64640	6.7e-22	29.38	Probable carboxylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=CXE8 PE=2 SV=1	[more]
O64641	5.8e-18	27.19	Probable carboxylesterase 9 OS=Arabidopsis thaliana OX=3702 GN=CXE9 PE=2 SV=1	[more]
A0A0A1EQ07	1.3e-17	31.88	3-O-acetylpapaveroxine carboxylesterase CXE2 OS=Papaver somniferum OX=3469 GN=CX...	[more]
I3PLR2	4.9e-17	29.72	3-O-acetylpapaveroxine carboxylesterase CXE1 OS=Papaver somniferum OX=3469 GN=CX...	[more]
Q0ZPV7	3.5e-15	28.57	Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1	[more]

BLAST of Clc05G02730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EC01	2.9e-52	41.95	probable carboxylesterase 8 OS=Cucurbita moschata OX=3662 GN=LOC111431817 PE=4 S...	[more]
A0A314ZB62	1.4e-30	33.65	Putative carboxylesterase 8 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn...	[more]
A0A6J5WIR3	5.3e-30	33.92	Abhydrolase_3 domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHA...	[more]
A0A6P5TG45	9.0e-30	31.76	probable carboxylesterase 8 OS=Prunus avium OX=42229 GN=LOC110766902 PE=4 SV=1	[more]
A0A6J5U0I2	9.0e-30	33.63	Abhydrolase_3 domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_L...	[more]

BLAST of Clc05G02730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45600.1	4.7e-23	29.38	alpha/beta-Hydrolases superfamily protein	[more]
AT2G45610.1	4.1e-19	27.19	alpha/beta-Hydrolases superfamily protein	[more]
AT5G62180.1	6.2e-15	26.06	carboxyesterase 20	[more]
AT5G23530.1	1.6e-10	38.82	carboxyesterase 18	[more]
AT5G16080.1	8.7e-09	31.43	carboxyesterase 17	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 17..247 e-value: 5.6E-19 score: 70.8
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 51..240
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 50..121 e-value: 1.9E-11 score: 44.3
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 52..129
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 194..240
None	No IPR available	PANTHER	PTHR23024:SF491	ALPHA/BETA HYDROLASE FOLD PROTEIN	coord: 52..129
None	No IPR available	PANTHER	PTHR23024:SF491	ALPHA/BETA HYDROLASE FOLD PROTEIN	coord: 194..240

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc05G02730.2	Clc05G02730.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity