Clc05G00100 (gene) Watermelon (cordophanus) v2

Overview
NameClc05G00100
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionS5A_REDUCTASE domain-containing protein
LocationClcChr05: 94939 .. 97594 (-)
RNA-Seq ExpressionClc05G00100
SyntenyClc05G00100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAAACCCTCCTGATCAACGGCAACCTAACCAGGAAATTGCAAACAGGAGTGCATTGGCTGTCCTGTTTGAATCAATGCTGCCTTGGGTTAACTATGGGGATGAAAGAGATGTTGGTGTTGAGGAAGGTAACCAATTCGATGAGCATGATGACCATTAAGTTGTCAATCCTTCTTGAGTCGCCGGTTCTCTTCAAATGCTTTGAGAGAATGGACTTAATGTGATGTGGCATAGATCACGTATGCGCAACTTGCCTTGTGTTGGAGCAGTTGTATCCAGTTTTGTTTACTGCTATCTGTCACTGGTTGCCAACCGATAATGCTTGGTACTGGTAGTTGTACTTGTATGTAGGAAGTTTTTGAATTCCCAACGCATTTGCTGTATTTCTCAAGCAAAGACGAACAATTCCCAAAATTGGAGGAAAATGTTTTCTTACTGGTTGGGGTGTATATAGAATAATAGAGAGGGCGTATGTTGTGGATGGTGCGGTTGTGAGGACTGACTGTGTCCCAATCATTACATACAGCTGCGTCAGTTGGATTCAGGAGATTTATAGTTCAGCCGGACGGCGCGGGGGGCCGCACCATCATTATGATTAGGCGCCCTTGCATTGCCCTGGCCCTGGCCCCCCAACCCATCATTTCGCTCGCACCCCAAACCAATTGCCAATAGCATGCAAAGTTATTTGCAGTGTCTTGATCATATGCTAATACCAAGCGTGCTTGTGGTCCTCTAATAAATAATTATCATAATTATTTTACTTGCTTAATAATTGTCATCATTATTATTTTACTTGCATGGTCCTCTTCTCTGCAGTAAGCCCCACTGCCAGAGGCTCTGCTAGTTGTTTTTGTTAAAATTAGGGGCCATTTTAAGTATTTCAATTGTGATTTGTGCAAAATGAAAATTTAATATCCGGTTATGATAGCATGCCAACTCTTCGTTACCATTTAAATTAATAGTACTATTACAATGAAAATCTACAGCTAATAGTTCATAAGATAGAGTGTAGGAACTGTGAAAGCAACACAAGTAGTGATAGAATGTGGGGATTACGGTTGTAATTTAACCAATTGTAATACAATAAATATAAATATGAAAGAAGACTTTTATTTTTAAATGGAGGGCTTTAGCACGTTAGCTTGCTTTTTCATCCTTTTCTAAGCATGTAATAAAAGTGAGAAGAAATTCGCCAGAGTAGGTCGGTTACAAAGTCAAAGAGAGCCAAAAGTCTGGTCGCAGCAGCAGCCCTATAAAAATGGATAGGGAAGAGAGCAATGAGGTGTGGGAGGCGTTGGCATAAGCGAAGAGACGGAAGGAAATAAAAGTTGGGAAACCCGATGGCCATGGCTAGCGGCTCAAATGGAAGTAATCTGAAGAATGGTGTCATAGCATTACTGGTTCCGCTTCCCTCCCATTCTCTTTTACCTCTGTTTCCTCAGCAAACTGTGGTGGTGGTAGCGGTCTCTGGAAATGGTGCAATCACCATCCTCTTTTGTTGGTGAATGCACTCTTCTTCCTCAATGTCAACCTTGTCTTCTGGCTCATCAGCCATCTCCAGTCCAGCCACTGGGTGAGACTCAAAATTCTTCTCAATTAAACTACACCTACTATATGTTATTAATTGGTGCACTGCAGATGATCGACCTGTACTGGACTTTGATACCCGTGATGCTGGTTCACTATTTTGCTTCTCACCCCTTAGCTGAGTATAATGAATTGAGGTCGTGGGTCGCAGTTGCTTTGACTTGGATTTGGAGCATAAGGCTTTCCCACAACTACTTAAGGCGGGAGGGATGGCAGTTGGGAGCCAGAGAGGACTGGAGGTTCACGGATATGCGGCGACAGTACGGAAAGAAACTGGTGGTGGGCTTCTTTCTTCGCCGTCTACCTTTCACAGCAGGTAATCTAATAACTAATCAAGGAATTAGCTTTCACATACTGGTGGTGGTGGTTGATTTGGTTTCAACTTTTGTACAGGTATTTCTGATGGGCGTATGCCTGCCTCTGTACGTTGTCCACTCAGTTGAGGAGGGATTGAAGTTGTGGGATTTGGTAGCCATCTTTGTGTGTGGTTGTGGAATTGGGATGGCCTACTTTGCTGACACCAGCTGCATGAATTCGTGAGCAGGAACAGGAAGCTGAAAATGAGTGGAAAATCGTTGGTGCCCATTCTTGAGGAGGGATTGTGGCGTTACTGTCGACACCCAAACTACTTTGGAGAGCAGTTGTGGTGGTGGGGGCTTGGGATATTGGGATGGGGTTTAGGTCAGAGATGGAGCCTTGCAGGAGCGTTGCTCAACACTATATGCTTAGCTTATGTAACCAAACTGGTTGAAGACCGAATGGTGAAACAACACTACAGAGCAGAAGCTTACAGGCAATATCAGAAGACCACATCAGTATGGATACCTTGGTTCAGGACATCACTTCACAAGGAAAAGAATATGTGACTTCACTTCTATCTTTTTATTATTATTATTATTATTTTAATGAATTCTCAGTTGTGGATGCACCGAGACTGAGCTGTGTTGTTTTATGTTATCTTGTTGTAATGTAAGTGTAACATTTCTCACGTGAATTATATTGTGCAACAAAAAGTACGGTTTTATCCGTTGTCTATTCTATCAATAAAACCGCCTTTGTCTTGTTTT

mRNA sequence

ATGCCAAACCCTCCTGATCAACGGCAACCTAACCAGGAAATTGCAAACAGGAGTGCATTGGCTGTCCTGTTTGAATCAATGCTGCCTTGGGTTAACTATGGGGATGAAAGAGATGTTGGTGTTGAGGAAGATCACGTATGCGCAACTTGCCTTGTGTTGGAGCAGTTGTATCCAGTTTTGTTTACTGCTATCTGTCACTGGTTGCCAACCGATAATGCTTGGTACTGCATTACTGGTTCCGCTTCCCTCCCATTCTCTTTTACCTCTGTTTCCTCAGCAAACTGTGGTGGTGGTAGCGGTCTCTGGAAATGGTGCAATCACCATCCTCTTTTGTTGGTGAATGCACTCTTCTTCCTCAATGTCAACCTTGTCTTCTGGCTCATCAGCCATCTCCAGTCCAGCCACTGGATGATCGACCTGTACTGGACTTTGATACCCGTGATGCTGGTTCACTATTTTGCTTCTCACCCCTTAGCTGAGTATAATGAATTGAGGTCGTGGGTCGCAGTTGCTTTGACTTGGATTTGGAGCATAAGGCTTTCCCACAACTACTTAAGGCGGGAGGGATGGCAGTTGGGAGCCAGAGAGGACTGGAGGTTCACGGATATGCGGCGACAGTACGGAAAGAAACTGGTGGTGGGCTTCTTTCTTCGCCGTCTACCTTTCACAGCAGGTATTTCTGATGGGCGTATGCCTGCCTCTCTGCATGAATTCGTGAGCAGGAACAGGAAGCTGAAAATGAGTGGAAAATCGTTGGTGCCCATTCTTGAGGAGGGATTGTGGCGTTACTGTCGACACCCAAACTACTTTGGAGAGCAGTTGTGGTGGTGGGGGCTTGGGATATTGGGATGGGGTTTAGGTCAGAGATGGAGCCTTGCAGGAGCGTTGCTCAACACTATATGCTTAGCTTATGTAACCAAACTGGTTGAAGACCGAATGGTGAAACAACACTACAGAGCAGAAGCTTACAGGCAATATCAGAAGACCACATCAGTATGGATACCTTGGTTCAGGACATCACTTCACAAGGAAAAGAATATGTGACTTCACTTCTATCTTTTTATTATTATTATTATTATTTTAATGAATTCTCAGTTGTGGATGCACCGAGACTGAGCTGTGTTGTTTTATGTTATCTTGTTGTAATGTAAGTGTAACATTTCTCACGTGAATTATATTGTGCAACAAAAAGTACGGTTTTATCCGTTGTCTATTCTATCAATAAAACCGCCTTTGTCTTGTTTT

Coding sequence (CDS)

ATGCCAAACCCTCCTGATCAACGGCAACCTAACCAGGAAATTGCAAACAGGAGTGCATTGGCTGTCCTGTTTGAATCAATGCTGCCTTGGGTTAACTATGGGGATGAAAGAGATGTTGGTGTTGAGGAAGATCACGTATGCGCAACTTGCCTTGTGTTGGAGCAGTTGTATCCAGTTTTGTTTACTGCTATCTGTCACTGGTTGCCAACCGATAATGCTTGGTACTGCATTACTGGTTCCGCTTCCCTCCCATTCTCTTTTACCTCTGTTTCCTCAGCAAACTGTGGTGGTGGTAGCGGTCTCTGGAAATGGTGCAATCACCATCCTCTTTTGTTGGTGAATGCACTCTTCTTCCTCAATGTCAACCTTGTCTTCTGGCTCATCAGCCATCTCCAGTCCAGCCACTGGATGATCGACCTGTACTGGACTTTGATACCCGTGATGCTGGTTCACTATTTTGCTTCTCACCCCTTAGCTGAGTATAATGAATTGAGGTCGTGGGTCGCAGTTGCTTTGACTTGGATTTGGAGCATAAGGCTTTCCCACAACTACTTAAGGCGGGAGGGATGGCAGTTGGGAGCCAGAGAGGACTGGAGGTTCACGGATATGCGGCGACAGTACGGAAAGAAACTGGTGGTGGGCTTCTTTCTTCGCCGTCTACCTTTCACAGCAGGTATTTCTGATGGGCGTATGCCTGCCTCTCTGCATGAATTCGTGAGCAGGAACAGGAAGCTGAAAATGAGTGGAAAATCGTTGGTGCCCATTCTTGAGGAGGGATTGTGGCGTTACTGTCGACACCCAAACTACTTTGGAGAGCAGTTGTGGTGGTGGGGGCTTGGGATATTGGGATGGGGTTTAGGTCAGAGATGGAGCCTTGCAGGAGCGTTGCTCAACACTATATGCTTAGCTTATGTAACCAAACTGGTTGAAGACCGAATGGTGAAACAACACTACAGAGCAGAAGCTTACAGGCAATATCAGAAGACCACATCAGTATGGATACCTTGGTTCAGGACATCACTTCACAAGGAAAAGAATATGTGA

Protein sequence

MPNPPDQRQPNQEIANRSALAVLFESMLPWVNYGDERDVGVEEDHVCATCLVLEQLYPVLFTAICHWLPTDNAWYCITGSASLPFSFTSVSSANCGGGSGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVMLVHYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGKKLVVGFFLRRLPFTAGISDGRMPASLHEFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGALLNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEKNM
Homology
BLAST of Clc05G00100 vs. NCBI nr
Match: XP_038893369.1 (uncharacterized protein C594.04c [Benincasa hispida])

HSP 1 Score: 442.6 bits (1137), Expect = 3.2e-120
Identity = 218/291 (74.91%), Postives = 227/291 (78.01%), Query Frame = 0

Query: 93  ANCG----GGSGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVM 152
           +NCG     GSGLWKWC HHPLLLVNALFFLNVNLVFWLISH+ SSHWMIDLYWTLIPVM
Sbjct: 34  SNCGDGNCSGSGLWKWCYHHPLLLVNALFFLNVNLVFWLISHVLSSHWMIDLYWTLIPVM 93

Query: 153 LVHYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYG 212
           LVHYFASHPLAEYNELRSW AVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYG
Sbjct: 94  LVHYFASHPLAEYNELRSWAAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYG 153

Query: 213 KK-LVVGFFLRRLP------------------------------FTAGISDGR---MPAS 272
           K    V FF   L                               F  G   G        
Sbjct: 154 KNWWWVSFFAVYLSQQVFLMGVCLPLYVVHSVEEGLKLWDLVAIFVCGSGIGMAYFADTQ 213

Query: 273 LHEFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAG 332
           LHEFVSRNRKLK+SGKS+VP L+EGLWRYCRHPNYFGEQLWWWGLGILGWGLGQ W LAG
Sbjct: 214 LHEFVSRNRKLKISGKSMVPNLQEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQGWCLAG 273

Query: 333 ALLNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEK 346
           +LLNT+CLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLH ++
Sbjct: 274 SLLNTMCLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHHKE 324

BLAST of Clc05G00100 vs. NCBI nr
Match: XP_004134275.1 (uncharacterized protein C594.04c [Cucumis sativus] >KGN56330.1 hypothetical protein Csa_011415 [Cucumis sativus])

HSP 1 Score: 441.8 bits (1135), Expect = 5.4e-120
Identity = 216/291 (74.23%), Postives = 229/291 (78.69%), Query Frame = 0

Query: 93  ANCGGG--SGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVMLV 152
           +NCGGG  SGLWKWC HHPLLLVNALFFLNVNL+FWLISH+QSSHWMIDLYWTLIPVMLV
Sbjct: 34  SNCGGGCSSGLWKWCYHHPLLLVNALFFLNVNLLFWLISHVQSSHWMIDLYWTLIPVMLV 93

Query: 153 HYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGKK 212
           HYFASHPLAEYNELRSWV VALTWIWSIRLSHNYLRREGWQLG REDWRFTDMR+QYGK 
Sbjct: 94  HYFASHPLAEYNELRSWVVVALTWIWSIRLSHNYLRREGWQLGTREDWRFTDMRQQYGKN 153

Query: 213 -LVVGFF---LRRLPFTAGISDGR------------------------------MPASLH 272
              V FF   L +  F  G+                                      LH
Sbjct: 154 WWWVSFFAVYLSQQVFLMGVCVPLYVVHSVKEELKLWDLVAIFICVSGIGMAYFADTQLH 213

Query: 273 EFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGAL 332
           EFVSRNRKLKMSGK++VP LEEGLWRYCRHPNYFGEQLWWWG+GILGWGLG  WSL G+L
Sbjct: 214 EFVSRNRKLKMSGKAMVPNLEEGLWRYCRHPNYFGEQLWWWGVGILGWGLGVGWSLVGSL 273

Query: 333 LNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEKNM 348
           LNT+CLAYVTKLVE RMVKQHYRAEAYRQYQKTTSVWIPWFR SL+K+KNM
Sbjct: 274 LNTMCLAYVTKLVEARMVKQHYRAEAYRQYQKTTSVWIPWFRKSLYKQKNM 324

BLAST of Clc05G00100 vs. NCBI nr
Match: KAG7028469.1 (Transcription factor 25 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441.0 bits (1133), Expect = 9.2e-120
Identity = 236/392 (60.20%), Postives = 269/392 (68.62%), Query Frame = 0

Query: 3   NPPDQRQPNQEIANRSALAVLFESMLPWVNYGDERDV--GVEEDHVCATCLVLEQLYPVL 62
           NPP+QR P +EIANRSALAVLFESMLPW   G  +    GV      A  ++ +     +
Sbjct: 591 NPPNQRHPTREIANRSALAVLFESMLPW---GRRQSAVGGVGISGAVARTIIEQDSDRKV 650

Query: 63  FTAICHWLPTD-----NAWYCITGSASLP-FSFTSVSSANCGGG--SGLWKWCNHHPLLL 122
             A+      +     NA   +     LP   F     +NCG G  +GLWKWC HHPLLL
Sbjct: 651 GKAMAMGSGRNGGNLKNAVVALL--VPLPSILFYLCFLSNCGNGTCTGLWKWCYHHPLLL 710

Query: 123 VNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVMLVHYFASHPLAEYNELRSWVAVAL 182
           VNALFFLNVN VFWL+SH+QSSHWMIDLYWTLIPVMLVHYFASHPLA YNELRSW+A+AL
Sbjct: 711 VNALFFLNVNFVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIAL 770

Query: 183 TWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGKK-LVVGFF---LRRLPFTAGIS- 242
           TWIWSIRL+HNYLRREGWQLG REDWRFTDMR+QYGK    V FF   L +  F  G+  
Sbjct: 771 TWIWSIRLTHNYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYLSQQIFLIGVCL 830

Query: 243 ------------------------DGRMPA-----SLHEFVSRNRKLKMSGKSLVPILEE 302
                                    G + A      LHEFV+RN +LKMSGKS+VP LEE
Sbjct: 831 PLYVVHSVDEALNMWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEE 890

Query: 303 GLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGALLNTICLAYVTKLVEDRMVKQHY 348
           GLWRY RHPNYFGEQLWWWGL I  WGLG+ WSL G+++N++CLAYVTKLVE+RMVKQ Y
Sbjct: 891 GLWRYSRHPNYFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEY 950

BLAST of Clc05G00100 vs. NCBI nr
Match: XP_008437759.1 (PREDICTED: uncharacterized protein C594.04c [Cucumis melo] >KAA0048799.1 uncharacterized protein E6C27_scaffold171G00110 [Cucumis melo var. makuwa] >TYK20751.1 uncharacterized protein E5676_scaffold291G00100 [Cucumis melo var. makuwa])

HSP 1 Score: 431.4 bits (1108), Expect = 7.3e-117
Identity = 212/292 (72.60%), Postives = 227/292 (77.74%), Query Frame = 0

Query: 93  ANCGGG---SGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVML 152
           +NCGGG   SGLWKWC HHPLLLVNALFFLNVNL+FWLIS +QSSHWMIDLYWTLIPV+L
Sbjct: 34  SNCGGGGCSSGLWKWCYHHPLLLVNALFFLNVNLLFWLISLVQSSHWMIDLYWTLIPVVL 93

Query: 153 VHYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGK 212
           VHYFASHPLAEYNELRSWV VALTWIWS RLSHNYLRREGWQLGAREDWRFTDMR+QYG+
Sbjct: 94  VHYFASHPLAEYNELRSWVVVALTWIWSTRLSHNYLRREGWQLGAREDWRFTDMRQQYGR 153

Query: 213 K-LVVGFF---LRRLPFTAGISDGR------------------------------MPASL 272
               V FF   L +  F  G+                                      L
Sbjct: 154 NWWWVSFFAVYLSQQVFLMGVCVPLYVVHSVKEELKLWDLVAIFMCVSGIGMAYFADTQL 213

Query: 273 HEFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGA 332
           HEFVSRNRKLKMSGK++VP LEEGLWRYCRHPNYFGEQLWWWG+GILGWGLG  WS  G+
Sbjct: 214 HEFVSRNRKLKMSGKAMVPNLEEGLWRYCRHPNYFGEQLWWWGVGILGWGLGVGWSPVGS 273

Query: 333 LLNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEKNM 348
           LLNT+CLAYVTKLVE RMVKQHYRAEAYRQYQKTTSVWIPWFR SL+K+KNM
Sbjct: 274 LLNTMCLAYVTKLVEARMVKQHYRAEAYRQYQKTTSVWIPWFRKSLYKQKNM 325

BLAST of Clc05G00100 vs. NCBI nr
Match: KAG6597001.1 (putative calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 416.8 bits (1070), Expect = 1.9e-112
Identity = 201/291 (69.07%), Postives = 225/291 (77.32%), Query Frame = 0

Query: 93  ANCGGG--SGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVMLV 152
           +NCG G  +GLWKWC HHPLLLVNALFFLNVN VFWL+SH+QSSHWMIDLYWTLIPVMLV
Sbjct: 34  SNCGNGTCTGLWKWCYHHPLLLVNALFFLNVNFVFWLLSHVQSSHWMIDLYWTLIPVMLV 93

Query: 153 HYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGKK 212
           HYFASHPLA YNELRSW+A++LTWIWSIRL+HNYLRREGWQLG REDWRFTDMR+QYGK 
Sbjct: 94  HYFASHPLAHYNELRSWIAISLTWIWSIRLTHNYLRREGWQLGVREDWRFTDMRQQYGKN 153

Query: 213 -LVVGFF---LRRLPFTAGIS-------------------------DGRMPA-----SLH 272
              V FF   L +  F  G+                           G + A      LH
Sbjct: 154 WWWVSFFAVYLSQQIFLIGVCLPLYVVHSVDEALNMWDLVAIFVCVSGIVIAYFADTQLH 213

Query: 273 EFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGAL 332
           EFV+RN +LKMSGKS+VP LEEGLWRY RHPNYFGEQLWWWGL I  WGLG+ WSL G++
Sbjct: 214 EFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIFAWGLGEEWSLVGSM 273

Query: 333 LNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEKNM 348
           +N++CLAYVTKLVE+RMVKQ YRAEAYRQYQKTTSVWIPWF TS+ K+KNM
Sbjct: 274 VNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVKKDKNM 324

BLAST of Clc05G00100 vs. ExPASy TrEMBL
Match: A0A0A0L2V4 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G116670 PE=4 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 2.6e-120
Identity = 216/291 (74.23%), Postives = 229/291 (78.69%), Query Frame = 0

Query: 93  ANCGGG--SGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVMLV 152
           +NCGGG  SGLWKWC HHPLLLVNALFFLNVNL+FWLISH+QSSHWMIDLYWTLIPVMLV
Sbjct: 34  SNCGGGCSSGLWKWCYHHPLLLVNALFFLNVNLLFWLISHVQSSHWMIDLYWTLIPVMLV 93

Query: 153 HYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGKK 212
           HYFASHPLAEYNELRSWV VALTWIWSIRLSHNYLRREGWQLG REDWRFTDMR+QYGK 
Sbjct: 94  HYFASHPLAEYNELRSWVVVALTWIWSIRLSHNYLRREGWQLGTREDWRFTDMRQQYGKN 153

Query: 213 -LVVGFF---LRRLPFTAGISDGR------------------------------MPASLH 272
              V FF   L +  F  G+                                      LH
Sbjct: 154 WWWVSFFAVYLSQQVFLMGVCVPLYVVHSVKEELKLWDLVAIFICVSGIGMAYFADTQLH 213

Query: 273 EFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGAL 332
           EFVSRNRKLKMSGK++VP LEEGLWRYCRHPNYFGEQLWWWG+GILGWGLG  WSL G+L
Sbjct: 214 EFVSRNRKLKMSGKAMVPNLEEGLWRYCRHPNYFGEQLWWWGVGILGWGLGVGWSLVGSL 273

Query: 333 LNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEKNM 348
           LNT+CLAYVTKLVE RMVKQHYRAEAYRQYQKTTSVWIPWFR SL+K+KNM
Sbjct: 274 LNTMCLAYVTKLVEARMVKQHYRAEAYRQYQKTTSVWIPWFRKSLYKQKNM 324

BLAST of Clc05G00100 vs. ExPASy TrEMBL
Match: A0A5D3DAX2 (S5A_REDUCTASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00100 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.5e-117
Identity = 212/292 (72.60%), Postives = 227/292 (77.74%), Query Frame = 0

Query: 93  ANCGGG---SGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVML 152
           +NCGGG   SGLWKWC HHPLLLVNALFFLNVNL+FWLIS +QSSHWMIDLYWTLIPV+L
Sbjct: 34  SNCGGGGCSSGLWKWCYHHPLLLVNALFFLNVNLLFWLISLVQSSHWMIDLYWTLIPVVL 93

Query: 153 VHYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGK 212
           VHYFASHPLAEYNELRSWV VALTWIWS RLSHNYLRREGWQLGAREDWRFTDMR+QYG+
Sbjct: 94  VHYFASHPLAEYNELRSWVVVALTWIWSTRLSHNYLRREGWQLGAREDWRFTDMRQQYGR 153

Query: 213 K-LVVGFF---LRRLPFTAGISDGR------------------------------MPASL 272
               V FF   L +  F  G+                                      L
Sbjct: 154 NWWWVSFFAVYLSQQVFLMGVCVPLYVVHSVKEELKLWDLVAIFMCVSGIGMAYFADTQL 213

Query: 273 HEFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGA 332
           HEFVSRNRKLKMSGK++VP LEEGLWRYCRHPNYFGEQLWWWG+GILGWGLG  WS  G+
Sbjct: 214 HEFVSRNRKLKMSGKAMVPNLEEGLWRYCRHPNYFGEQLWWWGVGILGWGLGVGWSPVGS 273

Query: 333 LLNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEKNM 348
           LLNT+CLAYVTKLVE RMVKQHYRAEAYRQYQKTTSVWIPWFR SL+K+KNM
Sbjct: 274 LLNTMCLAYVTKLVEARMVKQHYRAEAYRQYQKTTSVWIPWFRKSLYKQKNM 325

BLAST of Clc05G00100 vs. ExPASy TrEMBL
Match: A0A1S3AUR9 (uncharacterized protein C594.04c OS=Cucumis melo OX=3656 GN=LOC103483096 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.5e-117
Identity = 212/292 (72.60%), Postives = 227/292 (77.74%), Query Frame = 0

Query: 93  ANCGGG---SGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVML 152
           +NCGGG   SGLWKWC HHPLLLVNALFFLNVNL+FWLIS +QSSHWMIDLYWTLIPV+L
Sbjct: 34  SNCGGGGCSSGLWKWCYHHPLLLVNALFFLNVNLLFWLISLVQSSHWMIDLYWTLIPVVL 93

Query: 153 VHYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGK 212
           VHYFASHPLAEYNELRSWV VALTWIWS RLSHNYLRREGWQLGAREDWRFTDMR+QYG+
Sbjct: 94  VHYFASHPLAEYNELRSWVVVALTWIWSTRLSHNYLRREGWQLGAREDWRFTDMRQQYGR 153

Query: 213 K-LVVGFF---LRRLPFTAGISDGR------------------------------MPASL 272
               V FF   L +  F  G+                                      L
Sbjct: 154 NWWWVSFFAVYLSQQVFLMGVCVPLYVVHSVKEELKLWDLVAIFMCVSGIGMAYFADTQL 213

Query: 273 HEFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGA 332
           HEFVSRNRKLKMSGK++VP LEEGLWRYCRHPNYFGEQLWWWG+GILGWGLG  WS  G+
Sbjct: 214 HEFVSRNRKLKMSGKAMVPNLEEGLWRYCRHPNYFGEQLWWWGVGILGWGLGVGWSPVGS 273

Query: 333 LLNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEKNM 348
           LLNT+CLAYVTKLVE RMVKQHYRAEAYRQYQKTTSVWIPWFR SL+K+KNM
Sbjct: 274 LLNTMCLAYVTKLVEARMVKQHYRAEAYRQYQKTTSVWIPWFRKSLYKQKNM 325

BLAST of Clc05G00100 vs. ExPASy TrEMBL
Match: A0A6J1F9X4 (uncharacterized protein C594.04c OS=Cucurbita moschata OX=3662 GN=LOC111442154 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 9.0e-113
Identity = 201/291 (69.07%), Postives = 225/291 (77.32%), Query Frame = 0

Query: 93  ANCGGG--SGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVMLV 152
           +NCG G  +GLWKWC HHPLLLVNALFFLNVN VFWL+SH+QSSHWMIDLYWTLIPVMLV
Sbjct: 34  SNCGNGTCTGLWKWCYHHPLLLVNALFFLNVNFVFWLLSHVQSSHWMIDLYWTLIPVMLV 93

Query: 153 HYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGKK 212
           HYFASHPLA YNELRSW+A++LTWIWSIRL+HNYLRREGWQLG REDWRFTDMR+QYGK 
Sbjct: 94  HYFASHPLAHYNELRSWIAISLTWIWSIRLTHNYLRREGWQLGVREDWRFTDMRQQYGKN 153

Query: 213 -LVVGFF---LRRLPFTAGIS-------------------------DGRMPA-----SLH 272
              V FF   L +  F  G+                           G + A      LH
Sbjct: 154 WWWVSFFAVYLSQQIFLIGVCLPLYVVHSVDEALNMWDLVAIFVCVSGIVIAYFADTQLH 213

Query: 273 EFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGAL 332
           EFV+RN +LKMSGKS+VP LEEGLWRY RHPNYFGEQLWWWGL I  WGLG+ WSL G++
Sbjct: 214 EFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIFAWGLGEEWSLVGSM 273

Query: 333 LNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSLHKEKNM 348
           +N++CLAYVTKLVE+RMVKQ YRAEAYRQYQKTTSVWIPWF TS+ K+KNM
Sbjct: 274 VNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVKKDKNM 324

BLAST of Clc05G00100 vs. ExPASy TrEMBL
Match: A0A6J1I9U8 (uncharacterized protein C594.04c OS=Cucurbita maxima OX=3661 GN=LOC111472914 PE=4 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.9e-110
Identity = 200/294 (68.03%), Postives = 223/294 (75.85%), Query Frame = 0

Query: 93  ANCGGG--SGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPVMLV 152
           +NCG    +GLWKWC HHPLLLVNALFFLNVN VFWL+SH+QSSHWMIDLYWTLIPVMLV
Sbjct: 34  SNCGNDTCTGLWKWCYHHPLLLVNALFFLNVNFVFWLLSHVQSSHWMIDLYWTLIPVMLV 93

Query: 153 HYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGKK 212
           HYFASHPLA YNELRSW+A+ALTWIWSIRL+HNYLRREGWQLG REDWRFTDMR+QYGK 
Sbjct: 94  HYFASHPLAHYNELRSWIAIALTWIWSIRLTHNYLRREGWQLGVREDWRFTDMRQQYGKN 153

Query: 213 -LVVGFF---LRRLPFTAGIS-------------------------DGRMPA-----SLH 272
              V FF     +  F  G+                           G + A      LH
Sbjct: 154 WWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDEALNLWDLVAIFVCVSGIVIAYFADTQLH 213

Query: 273 EFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLAGAL 332
           EFV+RN +LKMSGKS+VP LEEGLWRY RHPNYFGEQLWWWGL I  WGLG+ WSL G++
Sbjct: 214 EFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIFAWGLGEEWSLVGSM 273

Query: 333 LNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRTSL---HKEKNM 348
           +N++CLAYVTKLVE+RMVKQ YRAEAYRQYQKTTSVWIPWF TS+    K+KNM
Sbjct: 274 VNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVKDDEKDKNM 327

BLAST of Clc05G00100 vs. TAIR 10
Match: AT2G46890.1 (Protein of unknown function (DUF1295) )

HSP 1 Score: 337.8 bits (865), Expect = 1.0e-92
Identity = 164/295 (55.59%), Postives = 199/295 (67.46%), Query Frame = 0

Query: 88  TSVSSANCGGGSGLWKWCNHHPLLLVNALFFLNVNLVFWLISHLQSSHWMIDLYWTLIPV 147
           ++ SS++    S +  WC +HPLLL N LFFLNVN++FW+I  LQSSHWMID+YWT+IPV
Sbjct: 27  SNYSSSSDSELSSIQSWCLNHPLLLANLLFFLNVNVLFWVIGLLQSSHWMIDVYWTVIPV 86

Query: 148 MLVHYFASHPLAEYNELRSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQY 207
           MLVHYFASHPL++YN+LRS + + LTWIWSIRL+HNY RRE W+ GAREDWRF D+R+QY
Sbjct: 87  MLVHYFASHPLSQYNKLRSMIVITLTWIWSIRLTHNYFRRENWEWGAREDWRFNDLRKQY 146

Query: 208 GKK---------------LVVG-------------------FFLRRLPFTAGISDGRMPA 267
           GK                 ++G                   F    +  T  +       
Sbjct: 147 GKHWWWLSFFSVYVSQQIFLIGICLPLYVIHSIDEPLNIWDFISSAICLTGIVMAYFADT 206

Query: 268 SLHEFVSRNRKLKMSGKSLVPILEEGLWRYCRHPNYFGEQLWWWGLGILGWGLGQRWSLA 327
            LHEFV+ N+KLK  GK  +P L+ GLWRY RHPNY GEQLWWWGL I  W LGQ W+L 
Sbjct: 207 QLHEFVTGNQKLKEQGKPKIPNLDSGLWRYSRHPNYLGEQLWWWGLVIFAWNLGQGWTLI 266

Query: 328 GALLNTICLAYVTKLVEDRMVKQHYRAEAYRQYQKTTSVWIPWFRT--SLHKEKN 347
           GAL+NT+CL YVT LVE RMVKQ YRAEAYR YQKTTSVWIPWF++  +  K+KN
Sbjct: 267 GALVNTLCLVYVTILVERRMVKQQYRAEAYRAYQKTTSVWIPWFKSHAAATKDKN 321

BLAST of Clc05G00100 vs. TAIR 10
Match: AT1G18180.1 (Protein of unknown function (DUF1295) )

HSP 1 Score: 69.7 bits (169), Expect = 5.2e-12
Identity = 65/196 (33.16%), Postives = 89/196 (45.41%), Query Frame = 0

Query: 165 RSWVAVALTWIWSIRLSHNYLRREGWQLGAREDWRFTDMRRQYGKKLVVGFFLR------ 224
           R  V   L  +W +RL    L R    L   ED RF DMR   GK +V   F        
Sbjct: 62  RQIVLTVLVVVWGLRLGLFLLMR---ILQWGEDRRFDDMRENIGKLVVFWIFQAVWVWTV 121

Query: 225 RLP--FTAGISDGRM--PAS-----------LHEFVSRNRKL--KMSGKSLVPILEEGLW 284
            LP  F    + GR+  PA            L E  +  +KL  K   ++     + G+W
Sbjct: 122 SLPVTFVNASNGGRLFQPADVIGWTMWVAGFLIEATADQQKLSFKKCPENKGKWCDVGVW 181

Query: 285 RYCRHPNYFGEQLWWWGLGILGW----GLGQRWSLAGALLNTICLAYVT--KLVEDRMVK 332
           +Y RHPNYFGE L WWGL +       GL +   + G +  T+ L +V+   L+E+   K
Sbjct: 182 KYSRHPNYFGEMLLWWGLYVASLPVLKGL-EYLVIIGPVFLTLLLLFVSGIPLLEESADK 241

BLAST of Clc05G00100 vs. TAIR 10
Match: AT1G73650.1 (Protein of unknown function (DUF1295) )

HSP 1 Score: 59.7 bits (143), Expect = 5.4e-09
Identity = 32/78 (41.03%), Postives = 46/78 (58.97%), Query Frame = 0

Query: 259 GLWRYCRHPNYFGEQLWWWGLGILGWGL---GQRWSLAGALLNTICLAYVT--KLVEDRM 318
           G+W+Y RHPNYFGE L WWG+ +    +    +   + G L  T+ L +V+   L+E   
Sbjct: 94  GVWKYSRHPNYFGEMLLWWGIFVAASPVLEGAEYLVIFGPLFLTLLLLFVSGIPLLEASA 153

Query: 319 VKQHYRAEAYRQYQKTTS 332
            K+H  + AYR Y+KTTS
Sbjct: 154 DKKHGNSGAYRSYKKTTS 171

BLAST of Clc05G00100 vs. TAIR 10
Match: AT1G73650.2 (Protein of unknown function (DUF1295) )

HSP 1 Score: 59.7 bits (143), Expect = 5.4e-09
Identity = 32/78 (41.03%), Postives = 46/78 (58.97%), Query Frame = 0

Query: 259 GLWRYCRHPNYFGEQLWWWGLGILGWGL---GQRWSLAGALLNTICLAYVT--KLVEDRM 318
           G+W+Y RHPNYFGE L WWG+ +    +    +   + G L  T+ L +V+   L+E   
Sbjct: 176 GVWKYSRHPNYFGEMLLWWGIFVAASPVLEGAEYLVIFGPLFLTLLLLFVSGIPLLEASA 235

Query: 319 VKQHYRAEAYRQYQKTTS 332
            K+H  + AYR Y+KTTS
Sbjct: 236 DKKHGNSGAYRSYKKTTS 253

BLAST of Clc05G00100 vs. TAIR 10
Match: AT1G73650.3 (Protein of unknown function (DUF1295) )

HSP 1 Score: 59.7 bits (143), Expect = 5.4e-09
Identity = 32/78 (41.03%), Postives = 46/78 (58.97%), Query Frame = 0

Query: 259 GLWRYCRHPNYFGEQLWWWGLGILGWGL---GQRWSLAGALLNTICLAYVT--KLVEDRM 318
           G+W+Y RHPNYFGE L WWG+ +    +    +   + G L  T+ L +V+   L+E   
Sbjct: 176 GVWKYSRHPNYFGEMLLWWGIFVAASPVLEGAEYLVIFGPLFLTLLLLFVSGIPLLEASA 235

Query: 319 VKQHYRAEAYRQYQKTTS 332
            K+H  + AYR Y+KTTS
Sbjct: 236 DKKHGNSGAYRSYKKTTS 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893369.13.2e-12074.91uncharacterized protein C594.04c [Benincasa hispida][more]
XP_004134275.15.4e-12074.23uncharacterized protein C594.04c [Cucumis sativus] >KGN56330.1 hypothetical prot... [more]
KAG7028469.19.2e-12060.20Transcription factor 25 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_008437759.17.3e-11772.60PREDICTED: uncharacterized protein C594.04c [Cucumis melo] >KAA0048799.1 unchara... [more]
KAG6597001.11.9e-11269.07putative calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L2V42.6e-12074.23S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G1166... [more]
A0A5D3DAX23.5e-11772.60S5A_REDUCTASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3AUR93.5e-11772.60uncharacterized protein C594.04c OS=Cucumis melo OX=3656 GN=LOC103483096 PE=4 SV... [more]
A0A6J1F9X49.0e-11369.07uncharacterized protein C594.04c OS=Cucurbita moschata OX=3662 GN=LOC111442154 P... [more]
A0A6J1I9U81.9e-11068.03uncharacterized protein C594.04c OS=Cucurbita maxima OX=3661 GN=LOC111472914 PE=... [more]
Match NameE-valueIdentityDescription
AT2G46890.11.0e-9255.59Protein of unknown function (DUF1295) [more]
AT1G18180.15.2e-1233.16Protein of unknown function (DUF1295) [more]
AT1G73650.15.4e-0941.03Protein of unknown function (DUF1295) [more]
AT1G73650.25.4e-0941.03Protein of unknown function (DUF1295) [more]
AT1G73650.35.4e-0941.03Protein of unknown function (DUF1295) [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010721Protein of unknown function DUF1295PFAMPF06966DUF1295coord: 247..330
e-value: 6.3E-25
score: 88.0
coord: 125..213
e-value: 3.5E-20
score: 72.5
IPR010721Protein of unknown function DUF1295PANTHERPTHR322513-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 234..342
coord: 90..216
NoneNo IPR availableGENE3D1.20.120.1630coord: 132..337
e-value: 5.0E-15
score: 57.7
NoneNo IPR availablePANTHERPTHR32251:SF27SUBFAMILY NOT NAMEDcoord: 234..342
coord: 90..216
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 243..286
score: 9.933291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc05G00100.1Clc05G00100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors