Clc04G00820 (gene) Watermelon (cordophanus) v2

Overview
NameClc04G00820
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionAdenylosuccinate synthetase, chloroplastic
LocationClcChr04: 2527384 .. 2531174 (+)
RNA-Seq ExpressionClc04G00820
SyntenyClc04G00820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACATCTCTTCTCTTGTTTTTGAGCCCACTCTTTTCTCCGCCGCCAAATCGCCGTCGCCGCCCTGTCGGAAACCCTTTTCTGTTCGTCATCCTGCACTCGTCTTCTGCTCATCTGCTAAGCCCACCGCGGCTGTTAGTTCTTCTGTCAGTGTGCCGGAGTCGGCGAAGAAGGACGGACTGAAACGAATTGGGGAACTCAGTCAAGTATCGGGCGTGTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGACATCTTGGCCCAGCACTTTGAAATCGTCGCTCGCTGTCAGGTAGTCTTCGAGATTTTATTCCTTTTGTCTTGTGTTGTTTTTCTGTTTCTTTAAATGATTTTGGTGTTTAGGTTTCCTTTTTTTTTTTTCCTTTCTTTCTTTTTTTTTATTTTTGCTATTCAATTTCGATTTGTTGTTTGACTTGCTTACCTGCCTCCCTGTGATTCTTGTTTTCTTCTGTTTCCTTACTTTCTCTTTGTTCTCTTTGTTCTTCCTTCGCTGTCCGGTATTTTGACATTTTGTCTCCATTTGTTTAACTTTCGTTTTGTTTTTGAAGCATATTAGAATCTGCTTGTAGTCTTTTGTTTATGTACATGTAATGGTGAAAGCCAAAGGGCATCTATGATTTATCTCTGTATTTTCACTTCATCTTTTCGAAGAGCCTATGACCCATTTTGATTCTATGTCACGTTTTTATGTGAAATCCTGATGTGCATGCTACTTTAGGTTTCTGGAACTATCTGAAAGTTCAAACCGATTTGCAGGGTGGGGCTAATGCTGGGCACACAATATATAATGCAGAAGGGAAGAAGTTTGCTCTTCATCTCATTCCCTCAGGGATCCTTAATGAGGATACTTTATGTGTTATTGGAAATGGAGTTGTGGTGCACGTGCCTGGTTTCTTTGAGGAAATCGACCGTCTAGAAGCAAGTGGAGTCTCGTGCAAGGGAAGGATCTTGGTATCTGACCGAGCCCATCTGTTATTTGATTTCCATCAGGAGGTGGATGGTCTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCTAGTAAAGTGATTAGAAATGGTATTAGAGTGGGTGATTTGAGGCACTTGGACACTCTTCCTCAGAAGCTGGACCTTCTACTCTCGGATGCTGCTTCGAGATTTAAGGATTTTAAATATACCCCAGAAATTCTGAAGGAAGAAGTTGAAAAGTACAAAAGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGTATTTCATTAATGAAGCCATCTCTGCAAATAAGAAGATTTTGGTTGAAGGTGGTCAAGCGACTATGCTTGACATTGACTTTGGAACTTATCCTTTTGTAACTTCCTCCAGCCCTTCGGCAGGTGGAATTTGCACTGGTCTTGGAATTGCTCCAAGAGTTGTTGGTGATCTTATAGGCGTGGTGAGTATCTTTTCATTCAATTTTTTGTGACTTTGTTTAACTGATGAAAGAATTCCAAGTTTTATTCACCTCTTCTCTCTTTGAGGATTTGTGTCTACTATATTCTCTTTCCTTTCTTGTAAATCATGAAAAATTAAGACAGACTCAAAAAGTCTACTTCCTATCCTTAGGCATTAATCCCTTCCTGGTTCTCCTTGGCAATATGTATGCAAACATACTAATTCATTTGCTGTATGTATTTAGTGTCTGCTTTAATGACATTATAAAATCATTTTTGTGGAAGCCATGGTATCTAGAAGAACCTTTGAAACTATCTAAATTATTATTCAACCTTGAAGAGATTACAATTCATTGATTTTATTCTTTCATGCATTAACACAAAGCCATGAGTGACTGTTTCTTTATTTCTTTCTTGATGGAAAACTGATTTATATATCGGATATGCATACCCACAAGGTTCTCTGTTGATCTTTGCAGGTGAAGGCATACACAACTAGAGTTGGTTCTGGCCCTTTTCCCACTGAGATCTTGGGAAAGGGAGGTGACCTACTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGTCGACCTCGTCGTTGTGGTTGGCTTGATATAGTTGCACTGAAATATTGCTGTCAAATTAATGGCTTCTCTTCTCTGAATCTTACGAAACTTGACGTCCTCTCAGAACTGTCTGAGATACAACTGGGAATTTCTTATTGGACACCTGATGGAACACCAGTCAAATCATTTCCCGCAGATCTTAGCCTTCTTGAACAAATAAAGGCAAGCAATATGCATCTTTTTCTTTTCCCCCATTTTTCCTTGCATTTTGTTAACTTTATACGAAGTTATTGGAATCTGCTTGAATATGGTTGTTGAGTTTATTTACGATTTATGAATTATTTTAGAAATCAGTTTATAATTCATGCTTAAACTAGATCTTCATTCTTTTTTTCCAAGGGAGGAAAAAGTTTTGATTCAGAGTTTTTATTTGAGATAGAAAAGAAAGCTTTTGACCAAATCCAATTGAATCATAGGTTACGTATATGAACTTTTGGTGAGTAAAAGAAACTCTGTATATTAGATCATAGGTGGATTTGTGGTTTTCTCAATGGAAGATCTAGATGTTCGATTTGTGCTTTGGGCAATTTGATCACTTGTCTCCATTCTTAGTTTGTTAAATGGGGGTGTCTTGGGTAGCCTTAATGATAAAGGTTTTTCAAATGGTGGCTTTCAGGAATTCTGAAGAAGAAATAAACAAAAATATAAGTATAAAAGATAAATAAAAGGGTGGACTTTGCAGAAGGCCTTTCCCATGTGCTCCCTCATCGTTACTTCAGTTAAAAATTTTGGGAGCATTGTGTTGCAAGATTTTAAATGCTTTTAGGTAGTTGATGTCTCCATAAATAACAATATTACTCAATTATTTATGCTTGGTGTGCATGGGTTTAGAAGACGAGCCAAAAACTTTATAGGTTGTCTATCATTCATTCTTCTGGTAAACTTTTCAAAAATCAGCGAAAAGTTTGTCTAGTGTTTCAAAAAAGATAAAGTCTGAATGCATTTTTGTGCTTGAATGTGTTCTGTGCTACTAAGATTTTGGACGTTGATTGTCATTTTTTTTCCTAAAAAAAAAAAGAGTATTTGATTGTCTTGAATCTGAATAATATTTCTCTCTCTCACTCTCTCTATATTATTATTATTATTTTGCATCTATTGAATTTATGGTTGATTTAGAATGCACTATAGAAAACAGTAATATTTCTAAAAGTTTCAAGTGAAAAATTAGTCAACCTGACTGTAGCTAATAGTTGAGACATTATAACATTGACCATGGTTGGAAGCTTGAATCTCCACTCCCAAATATAATTGATTGAAAAGAAAAAGTTTCAATTAAAAAATTAGCGTTCTTAATATTGTCTGAAGTTTTTTTGAGGCTGTCCATGGTTCTTGGCATTTCTCTTTTGCAACTGTATCCTATATTATTTGCCAATCATTTTCATGCCTTTTTTTGCTTCCAAATAATCAACTGAATTGGTAGCTGAGATGTTCTTAAATTTCAAGGTCTTCATCTCCTAAATCCAGAATCTTAACAATACTTGTGTTTTTTCCATTTGTTTTTCCTGTTTGCAGGTGGAATATGAAACAATTCCTGGATGGATGTGTGATATCTCTTCAGTAAGAGATTATTCTGACCTTCCTAAGGCTGCGAAAGAATATGTATCAAGGATTGAAGAGCTTGTGGGTATACCTGTCCATTACATTGGTGTCGGTCCAGGCCGTGATGCACTATTATACAAATGA

mRNA sequence

ATGAACATCTCTTCTCTTGTTTTTGAGCCCACTCTTTTCTCCGCCGCCAAATCGCCGTCGCCGCCCTGTCGGAAACCCTTTTCTGTTCGTCATCCTGCACTCGTCTTCTGCTCATCTGCTAAGCCCACCGCGGCTGTTAGTTCTTCTGTCAGTGTGCCGGAGTCGGCGAAGAAGGACGGACTGAAACGAATTGGGGAACTCAGTCAAGTATCGGGCGTGTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGACATCTTGGCCCAGCACTTTGAAATCGTCGCTCGCTGTCAGGGTGGGGCTAATGCTGGGCACACAATATATAATGCAGAAGGGAAGAAGTTTGCTCTTCATCTCATTCCCTCAGGGATCCTTAATGAGGATACTTTATGTGTTATTGGAAATGGAGTTGTGGTGCACGTGCCTGGTTTCTTTGAGGAAATCGACCGTCTAGAAGCAAGTGGAGTCTCGTGCAAGGGAAGGATCTTGGTATCTGACCGAGCCCATCTGTTATTTGATTTCCATCAGGAGGTGGATGGTCTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCTAGTAAAGTGATTAGAAATGGTATTAGAGTGGGTGATTTGAGGCACTTGGACACTCTTCCTCAGAAGCTGGACCTTCTACTCTCGGATGCTGCTTCGAGATTTAAGGATTTTAAATATACCCCAGAAATTCTGAAGGAAGAAGTTGAAAAGTACAAAAGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGTATTTCATTAATGAAGCCATCTCTGCAAATAAGAAGATTTTGGTTGAAGGTGGTCAAGCGACTATGCTTGACATTGACTTTGGAACTTATCCTTTTGTAACTTCCTCCAGCCCTTCGGCAGGTGGAATTTGCACTGGTCTTGGAATTGCTCCAAGAGTTGTTGGTGATCTTATAGGCGTGGTGAAGGCATACACAACTAGAGTTGGTTCTGGCCCTTTTCCCACTGAGATCTTGGGAAAGGGAGGTGACCTACTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGTCGACCTCGTCGTTGTGGTTGGCTTGATATAGTTGCACTGAAATATTGCTGTCAAATTAATGGCTTCTCTTCTCTGAATCTTACGAAACTTGACGTCCTCTCAGAACTGTCTGAGATACAACTGGGAATTTCTTATTGGACACCTGATGGAACACCAGTCAAATCATTTCCCGCAGATCTTAGCCTTCTTGAACAAATAAAGGCAAGCAATATGCATCTTTTTCTTTTCCCCCATTTTTCCTTGCATTTTGTTAACTTTATACGAAGTTATTGGAATCTGCTTGAATATGGTTACGTATATGAACTTTTGGTGGAATATGAAACAATTCCTGGATGGATGTGTGATATCTCTTCAGTAAGAGATTATTCTGACCTTCCTAAGGCTGCGAAAGAATATGTATCAAGGATTGAAGAGCTTGTGGGTATACCTGTCCATTACATTGGTGTCGGTCCAGGCCGTGATGCACTATTATACAAATGA

Coding sequence (CDS)

ATGAACATCTCTTCTCTTGTTTTTGAGCCCACTCTTTTCTCCGCCGCCAAATCGCCGTCGCCGCCCTGTCGGAAACCCTTTTCTGTTCGTCATCCTGCACTCGTCTTCTGCTCATCTGCTAAGCCCACCGCGGCTGTTAGTTCTTCTGTCAGTGTGCCGGAGTCGGCGAAGAAGGACGGACTGAAACGAATTGGGGAACTCAGTCAAGTATCGGGCGTGTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGACATCTTGGCCCAGCACTTTGAAATCGTCGCTCGCTGTCAGGGTGGGGCTAATGCTGGGCACACAATATATAATGCAGAAGGGAAGAAGTTTGCTCTTCATCTCATTCCCTCAGGGATCCTTAATGAGGATACTTTATGTGTTATTGGAAATGGAGTTGTGGTGCACGTGCCTGGTTTCTTTGAGGAAATCGACCGTCTAGAAGCAAGTGGAGTCTCGTGCAAGGGAAGGATCTTGGTATCTGACCGAGCCCATCTGTTATTTGATTTCCATCAGGAGGTGGATGGTCTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCTAGTAAAGTGATTAGAAATGGTATTAGAGTGGGTGATTTGAGGCACTTGGACACTCTTCCTCAGAAGCTGGACCTTCTACTCTCGGATGCTGCTTCGAGATTTAAGGATTTTAAATATACCCCAGAAATTCTGAAGGAAGAAGTTGAAAAGTACAAAAGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGTATTTCATTAATGAAGCCATCTCTGCAAATAAGAAGATTTTGGTTGAAGGTGGTCAAGCGACTATGCTTGACATTGACTTTGGAACTTATCCTTTTGTAACTTCCTCCAGCCCTTCGGCAGGTGGAATTTGCACTGGTCTTGGAATTGCTCCAAGAGTTGTTGGTGATCTTATAGGCGTGGTGAAGGCATACACAACTAGAGTTGGTTCTGGCCCTTTTCCCACTGAGATCTTGGGAAAGGGAGGTGACCTACTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGTCGACCTCGTCGTTGTGGTTGGCTTGATATAGTTGCACTGAAATATTGCTGTCAAATTAATGGCTTCTCTTCTCTGAATCTTACGAAACTTGACGTCCTCTCAGAACTGTCTGAGATACAACTGGGAATTTCTTATTGGACACCTGATGGAACACCAGTCAAATCATTTCCCGCAGATCTTAGCCTTCTTGAACAAATAAAGGCAAGCAATATGCATCTTTTTCTTTTCCCCCATTTTTCCTTGCATTTTGTTAACTTTATACGAAGTTATTGGAATCTGCTTGAATATGGTTACGTATATGAACTTTTGGTGGAATATGAAACAATTCCTGGATGGATGTGTGATATCTCTTCAGTAAGAGATTATTCTGACCTTCCTAAGGCTGCGAAAGAATATGTATCAAGGATTGAAGAGCTTGTGGGTATACCTGTCCATTACATTGGTGTCGGTCCAGGCCGTGATGCACTATTATACAAATGA

Protein sequence

MNISSLVFEPTLFSAAKSPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKDGLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDGTPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIPGWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK
Homology
BLAST of Clc04G00820 vs. NCBI nr
Match: XP_038882112.1 (LOW QUALITY PROTEIN: adenylosuccinate synthetase, chloroplastic [Benincasa hispida])

HSP 1 Score: 947.6 bits (2448), Expect = 4.6e-272
Identity = 470/528 (89.02%), Postives = 480/528 (90.91%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAKSPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKDG 60
           MNISSL FEPTLFSAA  PSP CR+P S RHPALV CSS KP AAVSSSVSVP+SA+KDG
Sbjct: 1   MNISSLAFEPTLFSAANXPSPACRRPXSARHPALVVCSSTKPAAAVSSSVSVPDSARKDG 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQV GVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVXGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF YTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG
Sbjct: 241 KDFNYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQ+G+SYW PDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQVGVSYWAPDGT 420

Query: 421 PVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIPG 480
           PVKSFPADLSLLEQIK                                    VEYET+PG
Sbjct: 421 PVKSFPADLSLLEQIK------------------------------------VEYETMPG 480

Query: 481 WMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           WMCDISS+RDYSDLPKAAK+Y+SRIEELVGIPVHYIGVGPGRDALLYK
Sbjct: 481 WMCDISSIRDYSDLPKAAKDYISRIEELVGIPVHYIGVGPGRDALLYK 492

BLAST of Clc04G00820 vs. NCBI nr
Match: XP_004144664.1 (adenylosuccinate synthetase, chloroplastic [Cucumis sativus] >KGN54874.1 hypothetical protein Csa_012933 [Cucumis sativus])

HSP 1 Score: 933.3 bits (2411), Expect = 9.0e-268
Identity = 466/529 (88.09%), Postives = 479/529 (90.55%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAK-SPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKD 60
           MNISSL FEPTLFSAA  SPS  CR+PFS RHP L+ CSSAK  AAVSSSVSVPES +KD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFSGRHPPLIVCSSAKTVAAVSSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+LKEEVE YKRYAERLEPFITDTVYFINEAISANK+ILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLKEEVENYKRYAERLEPFITDTVYFINEAISANKRILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDG 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG+SY TPDG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 TPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIP 480
           TPV+SFPADLSLLEQIK                                    VEYET+P
Sbjct: 421 TPVRSFPADLSLLEQIK------------------------------------VEYETMP 480

Query: 481 GWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           GWMCDISS+RDYSDLPKAAKEY+SRIEELVG+PVHYIGVGPGRDALLYK
Sbjct: 481 GWMCDISSIRDYSDLPKAAKEYISRIEELVGLPVHYIGVGPGRDALLYK 493

BLAST of Clc04G00820 vs. NCBI nr
Match: XP_022967784.1 (adenylosuccinate synthetase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 928.7 bits (2399), Expect = 2.2e-266
Identity = 464/528 (87.88%), Postives = 477/528 (90.34%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAKSPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKDG 60
           M+ISSL F+PTLFSAAK PSP CR+PFS RHPAL+ CSSAKP AAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLPSPACRRPFSGRHPALIVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLRE ELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLP KLDLLLSDAASRF
Sbjct: 181 VDGLREVELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPHKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF Y+PEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYSPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG+SY TPDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGT 420

Query: 421 PVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIPG 480
           PV+SFPADLSLLEQIK                                    VEYET+PG
Sbjct: 421 PVESFPADLSLLEQIK------------------------------------VEYETMPG 480

Query: 481 WMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           WMCDISS+RDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK
Sbjct: 481 WMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 492

BLAST of Clc04G00820 vs. NCBI nr
Match: XP_008442241.1 (PREDICTED: adenylosuccinate synthetase, chloroplastic [Cucumis melo] >KAA0060060.1 adenylosuccinate synthetase [Cucumis melo var. makuwa])

HSP 1 Score: 928.3 bits (2398), Expect = 2.9e-266
Identity = 465/529 (87.90%), Postives = 477/529 (90.17%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAK-SPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKD 60
           MNISSL FEPTLFSAA  SPS  CR+PF  R+P L  CSSAK  AAV SSVSVPES +KD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFYGRYPTLFVCSSAKTVAAVGSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+L+EEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLEEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDG 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG+SY TPDG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 TPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIP 480
           TPV+SFPADLSLLEQIK                                    VEYET+P
Sbjct: 421 TPVRSFPADLSLLEQIK------------------------------------VEYETMP 480

Query: 481 GWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           GWMCDISS+RDYSDLPKAAKEY+SRIEELVGIPVHYIGVGPGRDALLYK
Sbjct: 481 GWMCDISSIRDYSDLPKAAKEYISRIEELVGIPVHYIGVGPGRDALLYK 493

BLAST of Clc04G00820 vs. NCBI nr
Match: XP_022928609.1 (adenylosuccinate synthetase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 927.9 bits (2397), Expect = 3.8e-266
Identity = 465/528 (88.07%), Postives = 477/528 (90.34%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAKSPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKDG 60
           M+ISSL F+PTLFSAAK  SP CR+PFS RHPALV CSSAKP AAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF YTPEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG+SY  PDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420

Query: 421 PVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIPG 480
           PV+SFPADLSLLEQIK                                    VEYET+PG
Sbjct: 421 PVESFPADLSLLEQIK------------------------------------VEYETMPG 480

Query: 481 WMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           WMCDISS+RDYSDLPKAAKEYV+RIEELVGIPVHYIGVGPGRDALLYK
Sbjct: 481 WMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYIGVGPGRDALLYK 492

BLAST of Clc04G00820 vs. ExPASy Swiss-Prot
Match: B9IJ21 (Adenylosuccinate synthetase, chloroplastic OS=Populus trichocarpa OX=3694 GN=PURA PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 4.0e-226
Identity = 397/531 (74.76%), Postives = 437/531 (82.30%), Query Frame = 0

Query: 1   MNISSLVFEPT---LFSAAKSPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAK 60
           MN+SSL  E      + AA  P+     P   R+   V CSS KP+A  SSS+SV ES  
Sbjct: 1   MNLSSLRLESNPRWSYHAAHFPAHHGLNPSFRRN--FVSCSSIKPSA--SSSLSVAESFT 60

Query: 61  KDGLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKK 120
           +D   RI  LSQVSGVLG QWGDEGKGKLVDILA+HF+IVARCQGGANAGHTIYN+EGKK
Sbjct: 61  RDSASRIESLSQVSGVLGSQWGDEGKGKLVDILAEHFDIVARCQGGANAGHTIYNSEGKK 120

Query: 121 FALHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDF 180
           FALHL+PSGILNEDTLCVIGNGVVVH+PG F+EID LEA+GVSC GRILVSDRAHLLFDF
Sbjct: 121 FALHLVPSGILNEDTLCVIGNGVVVHLPGLFKEIDGLEANGVSCTGRILVSDRAHLLFDF 180

Query: 181 HQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA 240
           HQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRV DLRH+DT PQKLD LLSD A
Sbjct: 181 HQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVCDLRHMDTFPQKLDALLSDVA 240

Query: 241 SRFKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDI 300
           SRF+ FKY PE+LKEEVE+YKR+AERLEPFITDTV+F+NE+I+  KKILVEGGQATMLDI
Sbjct: 241 SRFESFKYGPEMLKEEVERYKRFAERLEPFITDTVHFMNESIAKKKKILVEGGQATMLDI 300

Query: 301 DFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLL 360
           DFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYT+RVGSGPFPTEILG+GGDLL
Sbjct: 301 DFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTSRVGSGPFPTEILGQGGDLL 360

Query: 361 RFAGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTP 420
           RFAGQEFGTTTGRPRRCGWLD+VALKY CQINGFSSLNLTKLDVLSE SEIQ+G+SY   
Sbjct: 361 RFAGQEFGTTTGRPRRCGWLDVVALKYVCQINGFSSLNLTKLDVLSEFSEIQIGVSYKQI 420

Query: 421 DGTPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYET 480
           DGTPV+SFP DL LLEQ+K                                    V+YE 
Sbjct: 421 DGTPVESFPGDLCLLEQLK------------------------------------VDYEV 480

Query: 481 IPGWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           +PGW  DISS+R Y+DLPKAA++YV RIEELVG+P+HYIG+GPGRDAL+YK
Sbjct: 481 LPGWKSDISSIRKYADLPKAAQQYVERIEELVGVPIHYIGIGPGRDALIYK 491

BLAST of Clc04G00820 vs. ExPASy Swiss-Prot
Match: Q96529 (Adenylosuccinate synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PURA PE=1 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 1.7e-224
Identity = 394/530 (74.34%), Postives = 440/530 (83.02%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAKSPSPPCRKPFSVRHP-ALVFCSSAKPTAAVSSSVSV-PESAKK 60
           M++SSL  +     A   P    R+   + HP + V CS+ +P  AVS+S+SV  +SA  
Sbjct: 1   MSLSSLTLDSNPRFAVGGPYH--RRYPPLHHPRSFVSCSAKRP--AVSASLSVAADSAAT 60

Query: 61  DGLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKF 120
           + L RIG LSQVSGVLGCQWGDEGKGKLVDILAQHF+IVARCQGGANAGHTIYN+EGKKF
Sbjct: 61  ESLGRIGSLSQVSGVLGCQWGDEGKGKLVDILAQHFDIVARCQGGANAGHTIYNSEGKKF 120

Query: 121 ALHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFH 180
           ALHL+PSGILNEDT CVIGNGVVVH+PG F+EID LE++GVSCKGRILVSDRAHLLFDFH
Sbjct: 121 ALHLVPSGILNEDTTCVIGNGVVVHLPGLFKEIDGLESNGVSCKGRILVSDRAHLLFDFH 180

Query: 181 QEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAS 240
           QEVDGLRE+ELAKSFIGTT+RGIGP YSSKVIRNGIRVGDLRH+DTLPQKLDLLLSDAA+
Sbjct: 181 QEVDGLRESELAKSFIGTTKRGIGPAYSSKVIRNGIRVGDLRHMDTLPQKLDLLLSDAAA 240

Query: 241 RFKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDID 300
           RF+ FKYTPE+L+EEVE YKRYA+RLEP+ITDTV+FIN++IS  KK+LVEGGQATMLDID
Sbjct: 241 RFQGFKYTPEMLREEVEAYKRYADRLEPYITDTVHFINDSISQKKKVLVEGGQATMLDID 300

Query: 301 FGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLR 360
           FGTYPFVTSSSPSAGGICTGLGIAP VVGDLIGVVKAYTTRVGSGPFPTE LG GGDLLR
Sbjct: 301 FGTYPFVTSSSPSAGGICTGLGIAPSVVGDLIGVVKAYTTRVGSGPFPTENLGTGGDLLR 360

Query: 361 FAGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPD 420
            AGQEFGTTTGRPRRCGWLDIVALK+ CQINGF+SLNLTKLDVLS+L+EIQLG++Y   D
Sbjct: 361 LAGQEFGTTTGRPRRCGWLDIVALKFSCQINGFASLNLTKLDVLSDLNEIQLGVAYKRSD 420

Query: 421 GTPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETI 480
           GTPVKSFP DL LLE                                    EL VEYE +
Sbjct: 421 GTPVKSFPGDLRLLE------------------------------------ELHVEYEVL 480

Query: 481 PGWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           PGW  DISSVR+YSDLPKAA++YV RIEELVG+P+HYIG+GPGRDAL+YK
Sbjct: 481 PGWKSDISSVRNYSDLPKAAQQYVERIEELVGVPIHYIGIGPGRDALIYK 490

BLAST of Clc04G00820 vs. ExPASy Swiss-Prot
Match: B9SL58 (Adenylosuccinate synthetase 2, chloroplastic OS=Ricinus communis OX=3988 GN=PURA2 PE=3 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 2.9e-224
Identity = 385/498 (77.31%), Postives = 415/498 (83.33%), Query Frame = 0

Query: 31  HPALVFCSSAKPTAAVSSSVSVPESAKKDGLKRIGELSQVSGVLGCQWGDEGKGKLVDIL 90
           H   V CS   P  +  S     +SA  D L RI  LSQVSGVLGCQWGDEGKGKLVDIL
Sbjct: 29  HRNFVVCSIKSP--STPSLNLATDSASTDSLSRINSLSQVSGVLGCQWGDEGKGKLVDIL 88

Query: 91  AQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEDTLCVIGNGVVVHVPGFFEE 150
           AQHF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNEDTLCVIGNGVVVH+PG F+E
Sbjct: 89  AQHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEDTLCVIGNGVVVHLPGLFKE 148

Query: 151 IDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVI 210
           ID LE+SGVSC GRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCY+SK I
Sbjct: 149 IDGLESSGVSCTGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYASKAI 208

Query: 211 RNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFKYTPEILKEEVEKYKRYAERLEPFITD 270
           RNGIRV DLRH+DT PQKLD+LLSDAASRF+ FKY PE+L+EEVEKYKRYAERLEPFI D
Sbjct: 209 RNGIRVSDLRHMDTFPQKLDILLSDAASRFQGFKYGPEMLREEVEKYKRYAERLEPFIAD 268

Query: 271 TVYFINEAISANKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLI 330
           TV+ +NE+I   KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPR VGDLI
Sbjct: 269 TVHVMNESIEQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRAVGDLI 328

Query: 331 GVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQING 390
           GVVKAYTTRVGSGPFPTEILG+GGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQING
Sbjct: 329 GVVKAYTTRVGSGPFPTEILGQGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQING 388

Query: 391 FSSLNLTKLDVLSELSEIQLGISYWTPDGTPVKSFPADLSLLEQIKASNMHLFLFPHFSL 450
           FSSLNLTKLDVLS+L EIQLG++Y   DGTP KSFPADL LLEQ+K              
Sbjct: 389 FSSLNLTKLDVLSDLPEIQLGVAYKHADGTPFKSFPADLRLLEQLK-------------- 448

Query: 451 HFVNFIRSYWNLLEYGYVYELLVEYETIPGWMCDISSVRDYSDLPKAAKEYVSRIEELVG 510
                                 VEYE +PGW  DISS+R+Y+D PKAA++YV RIEELVG
Sbjct: 449 ----------------------VEYEVLPGWKSDISSIRNYADFPKAAQQYVERIEELVG 488

Query: 511 IPVHYIGVGPGRDALLYK 529
           +PVHYIGVGPGRDAL+YK
Sbjct: 509 VPVHYIGVGPGRDALIYK 488

BLAST of Clc04G00820 vs. ExPASy Swiss-Prot
Match: A9XLE1 (Adenylosuccinate synthetase 1, chloroplastic OS=Capsicum frutescens OX=4073 GN=PURA1 PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 3.7e-224
Identity = 377/499 (75.55%), Postives = 426/499 (85.37%), Query Frame = 0

Query: 30  RHPALVFCSSAKPTAAVSSSVSVPESAKKDGLKRIGELSQVSGVLGCQWGDEGKGKLVDI 89
           ++P+++ CS+ KP A+V     V ES    GL RI  LSQVSGVLGCQWGDEGKGKLVDI
Sbjct: 50  KNPSIIVCSTTKPLASVVDRHGVSES----GLSRIESLSQVSGVLGCQWGDEGKGKLVDI 109

Query: 90  LAQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEDTLCVIGNGVVVHVPGFFE 149
           LA+HF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNE+T+CVIGNGVVVH+PG F+
Sbjct: 110 LAKHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEETVCVIGNGVVVHLPGLFK 169

Query: 150 EIDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKV 209
           EID LE++GVSC+GRILVSDRAHLLFDFHQE+DGLREAELAKSFIGTT+RGIGPCYSSKV
Sbjct: 170 EIDGLESNGVSCQGRILVSDRAHLLFDFHQEIDGLREAELAKSFIGTTKRGIGPCYSSKV 229

Query: 210 IRNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFKYTPEILKEEVEKYKRYAERLEPFIT 269
           IRNGIRV DLRH+DT PQKLD LLSDAA+RF  FKY PE+L+EEVE+YK++AERLEPFIT
Sbjct: 230 IRNGIRVSDLRHMDTFPQKLDRLLSDAAARFPGFKYGPEMLREEVERYKKFAERLEPFIT 289

Query: 270 DTVYFINEAISANKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDL 329
           DTV+F+N+AIS  KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDL
Sbjct: 290 DTVHFMNDAISQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDL 349

Query: 330 IGVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQIN 389
           +GVVKAYTTRVGSGPFPTEI+GKGGDLLRFAGQEFGTTTGRPRRCGWLDIVAL+YCCQIN
Sbjct: 350 VGVVKAYTTRVGSGPFPTEIMGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALRYCCQIN 409

Query: 390 GFSSLNLTKLDVLSELSEIQLGISYWTPDGTPVKSFPADLSLLEQIKASNMHLFLFPHFS 449
           GF+SLNLTKLDVLS+LSEIQLG++Y  PDG+ + SFP+DL LLEQ+K             
Sbjct: 410 GFASLNLTKLDVLSDLSEIQLGVTYRHPDGSILNSFPSDLRLLEQLK------------- 469

Query: 450 LHFVNFIRSYWNLLEYGYVYELLVEYETIPGWMCDISSVRDYSDLPKAAKEYVSRIEELV 509
                                  VEYE + GW  DISS+R YSDLPK+A+EYV RIEELV
Sbjct: 470 -----------------------VEYEVLRGWQSDISSIRKYSDLPKSAREYVERIEELV 508

Query: 510 GIPVHYIGVGPGRDALLYK 529
           G+P+HYIG+GPGRDAL+YK
Sbjct: 530 GVPIHYIGIGPGRDALIYK 508

BLAST of Clc04G00820 vs. ExPASy Swiss-Prot
Match: Q6T7E8 (Adenylosuccinate synthetase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PURA PE=2 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 5.4e-223
Identity = 380/497 (76.46%), Postives = 425/497 (85.51%), Query Frame = 0

Query: 32  PALVFCSSAKPTAAVSSSVSVPESAKKDGLKRIGELSQVSGVLGCQWGDEGKGKLVDILA 91
           P+++ CS+ KP A+V     V +S    GL RI  LSQVSGVLGCQWGDEGKGKLVDILA
Sbjct: 50  PSIIVCST-KPLASVVEHQGVNDS----GLTRIESLSQVSGVLGCQWGDEGKGKLVDILA 109

Query: 92  QHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEDTLCVIGNGVVVHVPGFFEEI 151
           +HF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNE+T+CVIGNGVVVH+PG F+EI
Sbjct: 110 KHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEETICVIGNGVVVHLPGLFKEI 169

Query: 152 DRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIR 211
           D LE++GVSC+GRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTT+RGIGPCYSSKVIR
Sbjct: 170 DNLESNGVSCQGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTKRGIGPCYSSKVIR 229

Query: 212 NGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFKYTPEILKEEVEKYKRYAERLEPFITDT 271
           NGIRV DLRH+DT PQ+LDLLLSDAASRF+ F Y  ++LKEEVE+YK++AERLEPFITDT
Sbjct: 230 NGIRVSDLRHMDTFPQQLDLLLSDAASRFQGFNYGRDMLKEEVERYKKFAERLEPFITDT 289

Query: 272 VYFINEAISANKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIG 331
           V+F+N+AIS  KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDL+G
Sbjct: 290 VHFMNDAISQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLVG 349

Query: 332 VVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQINGF 391
           VVKAYTTRVGSGPFPTEI+GKGGDLLRFAGQEFGTTTGRPRRCGWLDIVAL++CCQINGF
Sbjct: 350 VVKAYTTRVGSGPFPTEIMGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALRFCCQINGF 409

Query: 392 SSLNLTKLDVLSELSEIQLGISYWTPDGTPVKSFPADLSLLEQIKASNMHLFLFPHFSLH 451
           +SLNLTKLDVLS+LSEIQLG++Y  PDG+ + SFP+D+ LLEQIK               
Sbjct: 410 ASLNLTKLDVLSDLSEIQLGVTYRHPDGSTLNSFPSDIRLLEQIK--------------- 469

Query: 452 FVNFIRSYWNLLEYGYVYELLVEYETIPGWMCDISSVRDYSDLPKAAKEYVSRIEELVGI 511
                                VEYE +PGW  DISSVR YSDLPKAA+EYV RIEELVG+
Sbjct: 470 ---------------------VEYEVMPGWQSDISSVRKYSDLPKAAREYVERIEELVGV 505

Query: 512 PVHYIGVGPGRDALLYK 529
           PVHYIGVGPGRDAL+YK
Sbjct: 530 PVHYIGVGPGRDALIYK 505

BLAST of Clc04G00820 vs. ExPASy TrEMBL
Match: A0A0A0L1E1 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis sativus OX=3659 GN=PURA PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.4e-268
Identity = 466/529 (88.09%), Postives = 479/529 (90.55%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAK-SPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKD 60
           MNISSL FEPTLFSAA  SPS  CR+PFS RHP L+ CSSAK  AAVSSSVSVPES +KD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFSGRHPPLIVCSSAKTVAAVSSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+LKEEVE YKRYAERLEPFITDTVYFINEAISANK+ILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLKEEVENYKRYAERLEPFITDTVYFINEAISANKRILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDG 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG+SY TPDG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 TPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIP 480
           TPV+SFPADLSLLEQIK                                    VEYET+P
Sbjct: 421 TPVRSFPADLSLLEQIK------------------------------------VEYETMP 480

Query: 481 GWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           GWMCDISS+RDYSDLPKAAKEY+SRIEELVG+PVHYIGVGPGRDALLYK
Sbjct: 481 GWMCDISSIRDYSDLPKAAKEYISRIEELVGLPVHYIGVGPGRDALLYK 493

BLAST of Clc04G00820 vs. ExPASy TrEMBL
Match: A0A6J1HXQ5 (Adenylosuccinate synthetase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467197 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.1e-266
Identity = 464/528 (87.88%), Postives = 477/528 (90.34%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAKSPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKDG 60
           M+ISSL F+PTLFSAAK PSP CR+PFS RHPAL+ CSSAKP AAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLPSPACRRPFSGRHPALIVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLRE ELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLP KLDLLLSDAASRF
Sbjct: 181 VDGLREVELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPHKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF Y+PEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYSPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG+SY TPDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGT 420

Query: 421 PVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIPG 480
           PV+SFPADLSLLEQIK                                    VEYET+PG
Sbjct: 421 PVESFPADLSLLEQIK------------------------------------VEYETMPG 480

Query: 481 WMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           WMCDISS+RDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK
Sbjct: 481 WMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 492

BLAST of Clc04G00820 vs. ExPASy TrEMBL
Match: A0A5A7UY81 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=PURA PE=3 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.4e-266
Identity = 465/529 (87.90%), Postives = 477/529 (90.17%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAK-SPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKD 60
           MNISSL FEPTLFSAA  SPS  CR+PF  R+P L  CSSAK  AAV SSVSVPES +KD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFYGRYPTLFVCSSAKTVAAVGSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+L+EEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLEEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDG 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG+SY TPDG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 TPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIP 480
           TPV+SFPADLSLLEQIK                                    VEYET+P
Sbjct: 421 TPVRSFPADLSLLEQIK------------------------------------VEYETMP 480

Query: 481 GWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           GWMCDISS+RDYSDLPKAAKEY+SRIEELVGIPVHYIGVGPGRDALLYK
Sbjct: 481 GWMCDISSIRDYSDLPKAAKEYISRIEELVGIPVHYIGVGPGRDALLYK 493

BLAST of Clc04G00820 vs. ExPASy TrEMBL
Match: A0A1S3B4T1 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486156 PE=3 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.4e-266
Identity = 465/529 (87.90%), Postives = 477/529 (90.17%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAK-SPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKD 60
           MNISSL FEPTLFSAA  SPS  CR+PF  R+P L  CSSAK  AAV SSVSVPES +KD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFYGRYPTLFVCSSAKTVAAVGSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+L+EEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLEEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDG 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG+SY TPDG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 TPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIP 480
           TPV+SFPADLSLLEQIK                                    VEYET+P
Sbjct: 421 TPVRSFPADLSLLEQIK------------------------------------VEYETMP 480

Query: 481 GWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           GWMCDISS+RDYSDLPKAAKEY+SRIEELVGIPVHYIGVGPGRDALLYK
Sbjct: 481 GWMCDISSIRDYSDLPKAAKEYISRIEELVGIPVHYIGVGPGRDALLYK 493

BLAST of Clc04G00820 vs. ExPASy TrEMBL
Match: A0A6J1ELC2 (Adenylosuccinate synthetase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435468 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.8e-266
Identity = 465/528 (88.07%), Postives = 477/528 (90.34%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAKSPSPPCRKPFSVRHPALVFCSSAKPTAAVSSSVSVPESAKKDG 60
           M+ISSL F+PTLFSAAK  SP CR+PFS RHPALV CSSAKP AAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF YTPEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG+SY  PDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420

Query: 421 PVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETIPG 480
           PV+SFPADLSLLEQIK                                    VEYET+PG
Sbjct: 421 PVESFPADLSLLEQIK------------------------------------VEYETMPG 480

Query: 481 WMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           WMCDISS+RDYSDLPKAAKEYV+RIEELVGIPVHYIGVGPGRDALLYK
Sbjct: 481 WMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYIGVGPGRDALLYK 492

BLAST of Clc04G00820 vs. TAIR 10
Match: AT3G57610.1 (adenylosuccinate synthase )

HSP 1 Score: 780.0 bits (2013), Expect = 1.2e-225
Identity = 394/530 (74.34%), Postives = 440/530 (83.02%), Query Frame = 0

Query: 1   MNISSLVFEPTLFSAAKSPSPPCRKPFSVRHP-ALVFCSSAKPTAAVSSSVSV-PESAKK 60
           M++SSL  +     A   P    R+   + HP + V CS+ +P  AVS+S+SV  +SA  
Sbjct: 1   MSLSSLTLDSNPRFAVGGPYH--RRYPPLHHPRSFVSCSAKRP--AVSASLSVAADSAAT 60

Query: 61  DGLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKF 120
           + L RIG LSQVSGVLGCQWGDEGKGKLVDILAQHF+IVARCQGGANAGHTIYN+EGKKF
Sbjct: 61  ESLGRIGSLSQVSGVLGCQWGDEGKGKLVDILAQHFDIVARCQGGANAGHTIYNSEGKKF 120

Query: 121 ALHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFH 180
           ALHL+PSGILNEDT CVIGNGVVVH+PG F+EID LE++GVSCKGRILVSDRAHLLFDFH
Sbjct: 121 ALHLVPSGILNEDTTCVIGNGVVVHLPGLFKEIDGLESNGVSCKGRILVSDRAHLLFDFH 180

Query: 181 QEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAS 240
           QEVDGLRE+ELAKSFIGTT+RGIGP YSSKVIRNGIRVGDLRH+DTLPQKLDLLLSDAA+
Sbjct: 181 QEVDGLRESELAKSFIGTTKRGIGPAYSSKVIRNGIRVGDLRHMDTLPQKLDLLLSDAAA 240

Query: 241 RFKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDID 300
           RF+ FKYTPE+L+EEVE YKRYA+RLEP+ITDTV+FIN++IS  KK+LVEGGQATMLDID
Sbjct: 241 RFQGFKYTPEMLREEVEAYKRYADRLEPYITDTVHFINDSISQKKKVLVEGGQATMLDID 300

Query: 301 FGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLR 360
           FGTYPFVTSSSPSAGGICTGLGIAP VVGDLIGVVKAYTTRVGSGPFPTE LG GGDLLR
Sbjct: 301 FGTYPFVTSSSPSAGGICTGLGIAPSVVGDLIGVVKAYTTRVGSGPFPTENLGTGGDLLR 360

Query: 361 FAGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGISYWTPD 420
            AGQEFGTTTGRPRRCGWLDIVALK+ CQINGF+SLNLTKLDVLS+L+EIQLG++Y   D
Sbjct: 361 LAGQEFGTTTGRPRRCGWLDIVALKFSCQINGFASLNLTKLDVLSDLNEIQLGVAYKRSD 420

Query: 421 GTPVKSFPADLSLLEQIKASNMHLFLFPHFSLHFVNFIRSYWNLLEYGYVYELLVEYETI 480
           GTPVKSFP DL LLE                                    EL VEYE +
Sbjct: 421 GTPVKSFPGDLRLLE------------------------------------ELHVEYEVL 480

Query: 481 PGWMCDISSVRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 529
           PGW  DISSVR+YSDLPKAA++YV RIEELVG+P+HYIG+GPGRDAL+YK
Sbjct: 481 PGWKSDISSVRNYSDLPKAAQQYVERIEELVGVPIHYIGIGPGRDALIYK 490

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882112.14.6e-27289.02LOW QUALITY PROTEIN: adenylosuccinate synthetase, chloroplastic [Benincasa hispi... [more]
XP_004144664.19.0e-26888.09adenylosuccinate synthetase, chloroplastic [Cucumis sativus] >KGN54874.1 hypothe... [more]
XP_022967784.12.2e-26687.88adenylosuccinate synthetase, chloroplastic [Cucurbita maxima][more]
XP_008442241.12.9e-26687.90PREDICTED: adenylosuccinate synthetase, chloroplastic [Cucumis melo] >KAA0060060... [more]
XP_022928609.13.8e-26688.07adenylosuccinate synthetase, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B9IJ214.0e-22674.76Adenylosuccinate synthetase, chloroplastic OS=Populus trichocarpa OX=3694 GN=PUR... [more]
Q965291.7e-22474.34Adenylosuccinate synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU... [more]
B9SL582.9e-22477.31Adenylosuccinate synthetase 2, chloroplastic OS=Ricinus communis OX=3988 GN=PURA... [more]
A9XLE13.7e-22475.55Adenylosuccinate synthetase 1, chloroplastic OS=Capsicum frutescens OX=4073 GN=P... [more]
Q6T7E85.4e-22376.46Adenylosuccinate synthetase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PURA ... [more]
Match NameE-valueIdentityDescription
A0A0A0L1E14.4e-26888.09Adenylosuccinate synthetase, chloroplastic OS=Cucumis sativus OX=3659 GN=PURA PE... [more]
A0A6J1HXQ51.1e-26687.88Adenylosuccinate synthetase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5A7UY811.4e-26687.90Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B4T11.4e-26687.90Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034861... [more]
A0A6J1ELC21.8e-26688.07Adenylosuccinate synthetase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G57610.11.2e-22574.34adenylosuccinate synthase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001114Adenylosuccinate synthetaseSMARTSM00788adenylsucc_syntcoord: 70..527
e-value: 6.7E-233
score: 789.7
IPR001114Adenylosuccinate synthetaseTIGRFAMTIGR00184TIGR00184coord: 73..437
e-value: 7.0E-135
score: 448.1
IPR001114Adenylosuccinate synthetasePFAMPF00709Adenylsucc_syntcoord: 73..437
e-value: 1.2E-147
score: 492.2
coord: 472..524
e-value: 5.7E-15
score: 55.2
IPR001114Adenylosuccinate synthetasePANTHERPTHR11846ADENYLOSUCCINATE SYNTHETASEcoord: 30..438
coord: 469..528
IPR001114Adenylosuccinate synthetaseHAMAPMF_00011Adenylosucc_synthcoord: 1..528
score: 164.416168
IPR001114Adenylosuccinate synthetaseCDDcd03108AdSScoord: 69..527
e-value: 0.0
score: 534.386
IPR042111Adenylosuccinate synthetase, domain 3GENE3D3.90.170.10Adenylosuccinate Synthetase, subunit A, domain 3coord: 333..527
e-value: 2.1E-63
score: 214.6
IPR042109Adenylosuccinate synthetase, domain 1GENE3D3.40.440.10Adenylosuccinate Synthetase, subunit A, domain 1coord: 73..332
e-value: 1.4E-111
score: 374.4
IPR042110Adenylosuccinate synthetase, domain 2GENE3D1.10.300.10Adenylosuccinate Synthetase, subunit A, domain 2coord: 169..267
e-value: 1.4E-111
score: 374.4
NoneNo IPR availablePANTHERPTHR11846:SF14ADENYLOSUCCINATE SYNTHETASE, CHLOROPLASTICcoord: 469..528
NoneNo IPR availablePANTHERPTHR11846:SF14ADENYLOSUCCINATE SYNTHETASE, CHLOROPLASTICcoord: 30..438
IPR033128Adenylosuccinate synthase, active sitePROSITEPS00513ADENYLOSUCCIN_SYN_2coord: 200..211
IPR018220Adenylosuccinate synthase, GTP-binding sitePROSITEPS01266ADENYLOSUCCIN_SYN_1coord: 77..84
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 69..526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc04G00820.2Clc04G00820.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044208 'de novo' AMP biosynthetic process
biological_process GO:0046040 IMP metabolic process
biological_process GO:0006164 purine nucleotide biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004019 adenylosuccinate synthase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0000287 magnesium ion binding