Clc03G15480 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc03G15480
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	L-ascorbate oxidase
Location	ClcChr03: 27681480 .. 27685715 (+)
RNA-Seq Expression	Clc03G15480
Synteny	Clc03G15480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAAGTTGCAAATCAGCCATTATTTCCTATGCCTTTTCCAGTCATCTTCTTGGTTTTGTCAATCATTTCTGGGTTTGGAATAACACTCTCTGAGGCAGTTCCCATAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTCCCCGGACTGTGTCGAAAACGTCGTCATGGGAATCAACGGCCAGTTTCCTGGACCAACGATTAGAGCCAACGTTGGTGACACCACCGTTGTAGAGATGATTAACAAGCTCTCTACCGAAGGTCTCGTTATTCATTGGCATGGAATCCTGCAGAAGGGAACTCCTTGGGCTGATGGCACTGCTTCCATCTCGCAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGTGAGAAGGTTTTAAAATGTCAATGCTGATGAAGTGTCAAAAAAGTGATAAAATTTGTTTGTTCGTTGAACTCTTTTTTTTTTTTTTCCCCTGGCTGGGACGTATTTCTATCATGGCCACTTGGGGATGCAAAGAGCGGTAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAGGAGCCCTTCCATTACGATGAAGATATCAACTTATTGCTTAGTGATTGGTGGCATAAAAGCATTCAGAAGCAAGAAGTTGGCCTCACCTCCAACCCAATGCGTTGGATTGGTGATCCCGAGGTAGTTAATTTATGCTTCATCTACTAAATTATGTTTCAATTAACAATCATATTTCATTAAATCATACAAAATATTCATGATTGTGAGACAAGTTTCAATATTGTGCTTAACTATAAAAAGTTTCATTTTTTCTTTTTTTTTTTTTTGGGTATATTATGAAATTTATTTTATATATATATAACCTGTAGATGTGTTTTTCTTTCTTCTTCTTTTTCTTTTTTTTTTTTTTTTTTAACTATTTAAAAATAGATGAGGATGGAATGATGCTTAAGGTTTAAAAATTGAAATACCTACTTTAGTCTATTAACTAGTAAAATTTTTCTATATTTATAAATAGTTAGATATTTTTTCTATTCATAATCATTTCAATATATAGAGTTGTTTTCATATATAGTAAAATAAGTCAACTTATTTACAAATATAGCAAAATGACAAAAAAATAGTATATCATTGTCTATTAATTCAATCATTAGATTTTTTTTTTTTTTTCCTATATCAATTTGTCCATTTAAAACAACTCTTTGTTTGATAACCATTTATTTATTTATTTTTATAATTAATATGATTTCCTCTCAATTTAATATTCTAAAAAGAAAAACTAGTTTTCTATTAGAAAATATATAACAAAGCAAGATTTAGAAGTGAGAGAGGTGTTTTTAGGCTTACTTTTCAAAGATTAAAAAAAAAAAAAAAAACTAAGGCACTGTTTGATAACATAGTTGAATTCTTAACTACTACCAGAGTTGAATTCTTAACCACATTCTTAAAAATAAAAACAAGTTTTTAAAATTTATCTTATTAGTTTTCAAAACTTGCCTTAATTTTTGATAACATGGGTAAAAAATTAGATAAAAATAAATAATTTAAGGAGGGAAAAAGTGTTTAAATTTTTAGTTTAAAAAAAAATTAAAAACTCCAAGGAGAAATTACATTAATGATTACAACTTTAATAATCATCCAAACTCACACTTTTCAACTTATGTTGCTTTCTTTCATTAGACAGGAGAGAAAAATATTCAAAATCTTTGCTGCTTAAAAAAAAAAAAAAAACTTTTAATTTTGCATACAATAAATTTCTAAATTTTAGAAAATATCAAATACGTAAAAAATCTATTAGATTTATAATTAAAAATTCAATGATTTGTTAGAAACTTTTAAAGTTTAAAGATTTGTTAGACTCAAACTTAAAAGCTAATAGATTATAAACTTACAAGTTTACTTAGAAAAAGATTTATGGATCAGCTTGAATCTTTCCTATCACATATTTGTTGAACTCTAAAACAAATAAATAAATAATAAAAAAAAAAAAGCAAGATATACTTTTTTTTTAGTTAAATTAACTATTAAATAATTTAAAATTAAGAATACACACAAGGCTCAAACTACAAAGAATCAACAAAAGAACACACACATATCTTTTGGAGACATGTGTATAATTTAAGGTGTAATTTCCAACAAAAGAAAACATATTGGGAAAAAATATTCTCCCTCCTCTAAACTCCTTCCTTGCTTGAAATATGGTTTTTATGGTTTTTGTTATACATATAATATTAGACATATTATTCTGTAAACTTTTTTGCAAAGTTAAATTAGAATTATATAAGTTTTTTTTTTTTCTTTTGGTAAATTTCGAATTTTATATGTGTGTATGAAAAGAATGATGTAGACAACAACCCACATTTATTAGTTGTACACGTTAAAAAAAATATATATATATATATATATATATATATCTATATGTTTTCTAAAATTATAATATTACCTAGATCTTTTATAAACATTTCAAAACTACTCTTTAAGACATAAATTCTAAACGGAGACTTGAACACCAATTACACTATTCTAAGATAAATGGTTTCTATTTCAAAACTAATTTTGAAACATTTATAAATATAAAGAATATAAGAATATTTTTTAAACACATTGCAAATCTTAAAATATATTTTTTTCTTCCAATTTTGTCATATAATACATAAAAAAAAAAAAAAAAAAAAAAAACTTGGATTATATCTAACCATGTAAGAAAGTAAATATTTGGAAATTAAACCACCCTACATAAGTAAGTTTGGTTCTTTGTATTATTATTATTTTCTTTTTAACTTTGTCATTAGTGGTTGTATTAGGTAAAGTATTTATTAGAGATTTAATAATTGAACTATAGTGTTGTTGATTAATTAAATAGAATGTTTTTATAATATTTATGTTGTTGTTGTTGTTGTTGAAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGGTAACTCTCTCTCTCTCTTAAATGTTAAAATTGCTAAATAGTGATATTTTGTTATATTTATACAAATTTGGTTCTTTTGCTATATTTGATAATAACGTTGTTATTCTTGATTTTTAATATTTTTTTTAATCATTTTTCTTCTTCAGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAGCCCAAAGCCACCGGTGAAATCCAACCGCCGTATCTTTCTCCTCAACACCCAAAATCATATAAACGGCGGCTTCGTAAAGTGGGCCATCAATAACGTGTCTTTAGCCCTCCCCCCGACGCCGTACCTCGCCGCCATAAGAATCGGGCTAAACACCACCTTCGACCAAAATCCCCCACCAGAAACATTCCCAGAGGACTATGACATCTACAATCCACCACCGAACACGAACACGACGACCGGAAATGGCGTTTACCGATTCGAATTGTGGGAAATAGTGGATGTAATTCTACAAAATGCCAATACATTGGAGGATAAAAGAAGCGAGATTCACCCTTGGCATTTGCACGGCCATGATTTTTGGGTTTTGGGGTACGGAGAGGGGAAATTTTCCGCCGCCGTAGATGAGAAGAAATTGAATCTGAAAAACCCACCGCTGAGGAACACGGTGGTGATTTTCCCATATGGGTGGACGGCGATTCGATTTGTGGCTGATAATCCAGGAGTTTGGCCTTTCCATTGCCACATTGAACCTCATTTGCATATGGGAATGGGGGTTGTCTTCGCCGAAGGAGTTCATAAGGTCGACAGGATTCCGACGAAGGCTCTGGCTTGTGGTGAAACTGCGAAAGTGCTGATTAATAACCCCTCAAAAAAGCCCTAG

mRNA sequence

ATGGCAAAAGTTGCAAATCAGCCATTATTTCCTATGCCTTTTCCAGTCATCTTCTTGGTTTTGTCAATCATTTCTGGGTTTGGAATAACACTCTCTGAGGCAGTTCCCATAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTCCCCGGACTGTGTCGAAAACGTCGTCATGGGAATCAACGGCCAGTTTCCTGGACCAACGATTAGAGCCAACGTTGGTGACACCACCGTTGTAGAGATGATTAACAAGCTCTCTACCGAAGGTCTCGTTATTCATTGGCATGGAATCCTGCAGAAGGGAACTCCTTGGGCTGATGGCACTGCTTCCATCTCGCAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGTGAGAAGAGCGGTAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAGGAGCCCTTCCATTACGATGAAGATATCAACTTATTGCTTAGTGATTGGTGGCATAAAAGCATTCAGAAGCAAGAAGTTGGCCTCACCTCCAACCCAATGCGTTGGATTGGTGATCCCGAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAGCCCAAAGCCACCGGTGAAATCCAACCGCCGTATCTTTCTCCTCAACACCCAAAATCATATAAACGGCGGCTTCGTAAAGTGGGCCATCAATAACGTGTCTTTAGCCCTCCCCCCGACGCCGTACCTCGCCGCCATAAGAATCGGGCTAAACACCACCTTCGACCAAAATCCCCCACCAGAAACATTCCCAGAGGACTATGACATCTACAATCCACCACCGAACACGAACACGACGACCGGAAATGGCGTTTACCGATTCGAATTGTGGGAAATAGTGGATGTAATTCTACAAAATGCCAATACATTGGAGGATAAAAGAAGCGAGATTCACCCTTGGCATTTGCACGGCCATGATTTTTGGGTTTTGGGGTACGGAGAGGGGAAATTTTCCGCCGCCGTAGATGAGAAGAAATTGAATCTGAAAAACCCACCGCTGAGGAACACGGTGGTGATTTTCCCATATGGGTGGACGGCGATTCGATTTGTGGCTGATAATCCAGGAGTTTGGCCTTTCCATTGCCACATTGAACCTCATTTGCATATGGGAATGGGGGTTGTCTTCGCCGAAGGAGTTCATAAGGTCGACAGGATTCCGACGAAGGCTCTGGCTTGTGGTGAAACTGCGAAAGTGCTGATTAATAACCCCTCAAAAAAGCCCTAG

Coding sequence (CDS)

ATGGCAAAAGTTGCAAATCAGCCATTATTTCCTATGCCTTTTCCAGTCATCTTCTTGGTTTTGTCAATCATTTCTGGGTTTGGAATAACACTCTCTGAGGCAGTTCCCATAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTCCCCGGACTGTGTCGAAAACGTCGTCATGGGAATCAACGGCCAGTTTCCTGGACCAACGATTAGAGCCAACGTTGGTGACACCACCGTTGTAGAGATGATTAACAAGCTCTCTACCGAAGGTCTCGTTATTCATTGGCATGGAATCCTGCAGAAGGGAACTCCTTGGGCTGATGGCACTGCTTCCATCTCGCAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGTGAGAAGAGCGGTAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAGGAGCCCTTCCATTACGATGAAGATATCAACTTATTGCTTAGTGATTGGTGGCATAAAAGCATTCAGAAGCAAGAAGTTGGCCTCACCTCCAACCCAATGCGTTGGATTGGTGATCCCGAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAGCCCAAAGCCACCGGTGAAATCCAACCGCCGTATCTTTCTCCTCAACACCCAAAATCATATAAACGGCGGCTTCGTAAAGTGGGCCATCAATAACGTGTCTTTAGCCCTCCCCCCGACGCCGTACCTCGCCGCCATAAGAATCGGGCTAAACACCACCTTCGACCAAAATCCCCCACCAGAAACATTCCCAGAGGACTATGACATCTACAATCCACCACCGAACACGAACACGACGACCGGAAATGGCGTTTACCGATTCGAATTGTGGGAAATAGTGGATGTAATTCTACAAAATGCCAATACATTGGAGGATAAAAGAAGCGAGATTCACCCTTGGCATTTGCACGGCCATGATTTTTGGGTTTTGGGGTACGGAGAGGGGAAATTTTCCGCCGCCGTAGATGAGAAGAAATTGAATCTGAAAAACCCACCGCTGAGGAACACGGTGGTGATTTTCCCATATGGGTGGACGGCGATTCGATTTGTGGCTGATAATCCAGGAGTTTGGCCTTTCCATTGCCACATTGAACCTCATTTGCATATGGGAATGGGGGTTGTCTTCGCCGAAGGAGTTCATAAGGTCGACAGGATTCCGACGAAGGCTCTGGCTTGTGGTGAAACTGCGAAAGTGCTGATTAATAACCCCTCAAAAAAGCCCTAG

Protein sequence

MAKVANQPLFPMPFPVIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGETFIYRFVVDKVRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP

Homology

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BLAST of Clc03G15480 vs. NCBI nr
Match: XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])

HSP 1 Score: 1039.6 bits (2687), Expect = 9.8e-300
Identity = 486/590 (82.37%), Postives = 528/590 (89.49%), Query Frame = 0


Query: 1   MAKVANQPLFPMPFPVIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVM 60
           MAK ANQPLFP+PF VIFL+ S+ISGFGITLSEAV   R++KWEVEYMFWSPDCVEN+VM
Sbjct: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60

Query: 61  GINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNP 120
           GINGQFPGPTIRANVGDT +VE++NKL TEG+VIHWHGILQ+ TPWADGTASISQCA NP
Sbjct: 61  GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120

Query: 121 GETFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWW 180
           GETFIY+F  DK           ++R+ GLYGSLI+D EEGKKEPFHYDE+INLLLSDWW
Sbjct: 121 GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW 180

Query: 181 HKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFI 240
           HKS  KQEVGL+S+PMRWIG+PE ILINGRGQ +CSIAAKYN +L+QC+LKG EQCAPFI
Sbjct: 181 HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI 240

Query: 241 LHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVL 300
           L VKP KTYRIRIASTTSLSALN AI NHQLLVVEADGNYV+PF+TSDIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 300

Query: 301 ITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKN 360
           ITT+Q  ++NYWVSVGVR R PKTPPALT+LNYLPNSV+KLP+SPPPE PNW+DY RSKN
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 360

Query: 361 FTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGL 420
           FTYRILAAMGSP PP+K NRRIFLLNTQN +NGGF KWAINNVSL+LP TPYL AI+IGL
Sbjct: 361 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 420

Query: 421 NTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEI 480
           N TF+QNPPPETF EDYDI+NPPPN NT T NGVY F L E VDVILQNANTL DK SEI
Sbjct: 421 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
           HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540

Query: 541 PFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
            FHCHIEPHLHMGMGV+FAEGVHKV  IPTKALACG TAKVLINNP++KP
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 590

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BLAST of Clc03G15480 vs. NCBI nr
Match: XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1025.8 bits (2651), Expect = 1.5e-295
Identity = 487/592 (82.26%), Postives = 528/592 (89.19%), Query Frame = 0


Query: 1   MAKVANQPLFPMPFPVIFLVLSIISGFGITLSEA-VPIVRHYKWEVEYMFWSPDCVENVV 60
           MAKVA++ LFP PF +IF +LSII GFGITLSEA  P +RHYKWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKSLFPKPF-LIFFILSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATN 120
           MGI+GQFPGPTIRANVGDT VVE+ NKL TEG+VIHWHGI+Q+GTPWADGTASISQCA N
Sbjct: 61  MGIDGQFPGPTIRANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDK           ++R+ GLYGSLIVD EEGK EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDW 180

Query: 181 WHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH+S+ KQEVGL+S P+RWIG+P+ ILINGRGQ +CSIAAKYN +LKQCELKG EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV PNKTYRIRIASTT+L++LNFAI NH+LLVVEADGNY+QPFLTSDIDIYSGESYSV
Sbjct: 241 ILHVLPNKTYRIRIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQP+SENYWVS+GVRAR PKTPP LTILNYLPNSVSKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQPSSENYWVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPP++ NRRIFLLNTQN +N GFVKWAINNVSLALPPTPYLAA++  
Sbjct: 361 NFTFRIFAAMGSPKPPMRYNRRIFLLNTQNRMN-GFVKWAINNVSLALPPTPYLAAMKFK 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           LNT FDQNPPPETFP DYDI NPP N NT TGNGVYRF + EIVDVILQNAN L D  SE
Sbjct: 421 LNTAFDQNPPPETFPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLI-NNPSKKP 580
           W FHCHIEPHLHMGMGVVFAEGVHKV RIPTKALACG  AK LI NNP + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGAAKALINNNPPQDP 590

[Close]

BLAST of Clc03G15480 vs. NCBI nr
Match: NP_001284440.1 (L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucumis melo])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.7e-294
Identity = 476/588 (80.95%), Postives = 520/588 (88.44%), Query Frame = 0


Query: 3   KVANQPLFPMPFPVIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGI 62
           KVANQPLFPMPFP IF + SI  GFGITLS+A    RH+KWEVEYMF SPDC EN+VMGI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGE 122
           N QFPGPTIRANVGDT VVE+INKLSTE +VIHWHGILQKGTPWADGTASISQCA NPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHK 182
           +F Y+FVVDK           ++RA GLYGSLIVDLEEGKKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILH 242
           S+ KQEVGL+S+PMRWIG+P++ILINGRGQ+NCSI AKY  +++QC+L G E+CAPFIL 
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
           VKPNKTYRIRI STTSLSALNFAI NH+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFT 362
           TDQ  S+NYWVS+GVR R PKTPP LT+LNYLPNS SKLPISPPPE P W+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNT 422
           YRILAAMGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL LP TPYL AI+IG+N 
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHP 482
           TFDQNPPPET+PE+YDI+ PPPN NT TGNGVYRF+L EIVDV+LQNANTL DK SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NP++KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

[Close]

BLAST of Clc03G15480 vs. NCBI nr
Match: TYK09892.1 (L-ascorbate oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.2e-292
Identity = 475/588 (80.78%), Postives = 518/588 (88.10%), Query Frame = 0


Query: 3   KVANQPLFPMPFPVIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGI 62
           K ANQPLFPMPFP IF + SI  GFGITLS+A    RH+KWEVEYMF SPDC EN+VMGI
Sbjct: 4   KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGE 122
           N QFPGPTIRANVGDT VVE+INKLSTE +VIHWHGILQKGTPWADGTASISQCA NPGE
Sbjct: 64  NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHK 182
           +F Y+FVVDK           ++RA GLYGSLIVDLEEGKKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILH 242
           S+ KQEVGL+S+PMRWIG+P++ILINGRGQ+NCSI AKY  +++QC+L G E CAPFIL 
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243

Query: 243 VKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
           VKPNKTYRIRI STTSLSALNFAI NH+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFT 362
           TDQ  S+NYWVS+GVR R PKTPP LT+LNYLPNS SKLPISPPPE P W+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNT 422
           YRILAAMGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL LP TPYL AI+IG+N 
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHP 482
           TFDQNPPPET+PE+YDI+ PPPN NT TGNGVYRF+L EIVDV+LQNANTL DK SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NP++KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

[Close]

BLAST of Clc03G15480 vs. NCBI nr
Match: KAA0055636.1 (L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 981.5 bits (2536), Expect = 3.2e-282
Identity = 465/591 (78.68%), Postives = 510/591 (86.29%), Query Frame = 0


Query: 1   MAKVANQPLFPMPFPVIFLVLSIISGFGITLSEA-VPIVRHYKWEVEYMFWSPDCVENVV 60
           MAKVA++P FP PF ++F VLSII GFGITLSEA  P +RHYKWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPF-LVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATN 120
           MGINGQFPGPTIRAN GD  VVE+ NKL TEG+VIHWHGILQ+GTPWADGTASISQCA N
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDK           ++R+ GLYGSLIVD +EG  EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH+S+ KQEVGL+S PMRWIG+P++ILINGRGQ +CSIAAKY   LKQCEL G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV P KTYRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ  SENYWVS+GVRAR PKTPP LT+LNYLPNSVSKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA++  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           L+T F+QNPPPETFP DYDI NPP N  T TGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           W FHCHIEPHLHMGMGV+FAEGVHKV  IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

[Close]

BLAST of Clc03G15480 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 2.1e-284
Identity = 463/591 (78.34%), Postives = 512/591 (86.63%), Query Frame = 0


Query: 1   MAKVANQPLFPMPFPVIFLVLSIISGFGITLSEA-VPIVRHYKWEVEYMFWSPDCVENVV 60
           MAKVA++P FP PF + FLVLSII GFGITLSEA  P ++HYKW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPF-LSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATN 120
           MGING+FPGPTIRAN GD  VVE+ NKL TEG+VIHWHGILQ+GTPWADGTASISQCA N
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDK           ++R+ GLYGSLIVD  EG+ EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH+S+ KQEVGL+S PMRWIG+P++ILING+GQ +CSIAAKYN  LKQCEL G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV+P KTYRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ   ENYWVS+GVRAR PKTPP LT+LNYLPNS SKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA+++ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           LNT F+QNPPPETFP +YDI NPPPN  TTTGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           W FHCHIEPHLHMGMGVVFAEGVH V  IP KALACG TA  L+ N  + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587

[Close]

BLAST of Clc03G15480 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 924.9 bits (2389), Expect = 4.6e-268
Identity = 439/571 (76.88%), Postives = 482/571 (84.41%), Query Frame = 0


Query: 16  VIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANV 75
           +I     +I  FGI+ SE   I RHYKWEVEYMFW+PDC EN+VMGINGQFPGPTIRAN 
Sbjct: 13  LILFFFGLILAFGIS-SEGSQI-RHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANA 72

Query: 76  GDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGETFIYRFVVDK--- 135
           GDT VVE+INKL TEG+VIHWHGILQ+GTPWADGTASISQCA NPGETF Y F VD    
Sbjct: 73  GDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGT 132

Query: 136 --------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTSNP 195
                   ++R+ GLYGSLIVD  +GKKEPFHYD +INLLLSDWWH+SI KQEVGL+S P
Sbjct: 133 FFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKP 192

Query: 196 MRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRIAS 255
           +RWIG+P+ IL+NGRGQ +CSIAAKY+++L+ C+LKG E CAP+I HV P KTYRIRIAS
Sbjct: 193 IRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIAS 252

Query: 256 TTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSV 315
           TT+L+ALNFAI NH LLVVEADGNYVQPF TSDIDIYSGESYSVLITTDQ  SENYWVSV
Sbjct: 253 TTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSV 312

Query: 316 GVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPP 375
           G R R P TPP LT+LNYLPNSVSKLP SPPPE P WDD+DRSKNFTYRI AAMGSPKPP
Sbjct: 313 GTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSPKPP 372

Query: 376 VKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPE 435
           VKSNRRIFLLNTQN IN G+VKWAIN+VSLALPPTPYL A++  L   FDQNPPPE FPE
Sbjct: 373 VKSNRRIFLLNTQNVIN-GYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFPE 432

Query: 436 DYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGY 495
           DYDI  PP N  T  GNGVY+F++ EIVDVILQNAN +++  SEIHPWHLHGHDFWVLGY
Sbjct: 433 DYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGY 492

Query: 496 GEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMG 555
           G+GKF+A  +E  LNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHMGMG
Sbjct: 493 GDGKFTAE-EESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMG 552

Query: 556 VVFAEGVHKVDRIPTKALACGETAKVLINNP 576
           VVFAEGV KV RIPTKALACG TAK LINNP
Sbjct: 553 VVFAEGVEKVGRIPTKALACGGTAKSLINNP 579

[Close]

BLAST of Clc03G15480 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 1.3e-265
Identity = 427/549 (77.78%), Postives = 471/549 (85.79%), Query Frame = 0


Query: 38  VRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWH 97
           +RHYKWEVEYMFW+P+C EN+VMGINGQFPGPTIRAN GD+ VVE+ NKL TEG+VIHWH
Sbjct: 3   IRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWH 62

Query: 98  GILQKGTPWADGTASISQCATNPGETFIYRFVVDK-----------VRRAVGLYGSLIVD 157
           GILQ+GTPWADGTASISQCA NPGETF Y F VD            ++R+ GLYGSLIVD
Sbjct: 63  GILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVD 122

Query: 158 LEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSI 217
             +GKKEPFHYD +INLLLSDWWH+SI KQEVGL+S P+RWIG+P+ IL+NGRGQ +CSI
Sbjct: 123 PPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSI 182

Query: 218 AAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEAD 277
           AAKY+++L+ C+LKG E CAP+I HV P KTYRIRIASTT+L+ALNFAI NHQLLVVEAD
Sbjct: 183 AAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEAD 242

Query: 278 GNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNS 337
           GNYVQPF TSDIDIYSGESYSVLITTDQ  SENYWVSVG RAR P TPP LT+LNYLPNS
Sbjct: 243 GNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNS 302

Query: 338 VSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVK 397
           VSKLP SPPP+ P WDD+DRSKNFTYRI AAMGSPKPPVK NRRIFLLNTQN IN G+VK
Sbjct: 303 VSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVIN-GYVK 362

Query: 398 WAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRF 457
           WAIN+VSLALPPTPYL A++  L   FDQNPPPE FPEDYDI  PP N  T  GNGVY+F
Sbjct: 363 WAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQF 422

Query: 458 ELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLR 517
           ++ E+VDVILQNAN +++  SE HPWHLHGHDFWVLGYG+GKFSA  +E  LNLKNPPLR
Sbjct: 423 KIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAE-EESSLNLKNPPLR 482

Query: 518 NTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGE 576
           NTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHMGMGVVFAEGV KV RIPTKALACG 
Sbjct: 483 NTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGG 542

[Close]

BLAST of Clc03G15480 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 2.8e-225
Identity = 366/573 (63.87%), Postives = 440/573 (76.79%), Query Frame = 0


Query: 14  FPVIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRA 73
           F   FL+  I+     + S      RH+KW+VEY+ WSPD  E+VVMGINGQFPGPTIRA
Sbjct: 6   FLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 65

Query: 74  NVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGETFIYRFVVDK- 133
             GDT  V + NKL TEG+VIHWHGI Q GTPWADGTA+ISQCA NPGETF+YRF VDK 
Sbjct: 66  KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 125

Query: 134 ----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTS 193
                     ++R+ GLYGSLIV++ EG+KEPFHYD + NLLLSDWWHK   +QEV L+S
Sbjct: 126 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS 185

Query: 194 NPMRWIGDPEAILINGRGQTNCSIAAKYN-TSLKQCELKGDEQCAPFILHVKPNKTYRIR 253
           NP+RWIG+P+ +L+NGRGQ NCS+AA+++   L QC+L+G EQ AP IL V+PNK YR+R
Sbjct: 186 NPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 245

Query: 254 IASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYW 313
           +ASTT+L +L+ AI  H+++VVEADGNYVQPF   D+DIYSGESYSVL  TDQ  ++NYW
Sbjct: 246 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 305

Query: 314 VSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSP 373
           +S+ VR R PKTP  LT+LNYLPNS SK P  PPP  P W+DY+ SK+F+ +I A MGSP
Sbjct: 306 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSP 365

Query: 374 KPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPET 433
           KPP +++RRI LLNTQN I+ G+ KWAINNVSL LP   YL +IR G+N  FD  PPP+ 
Sbjct: 366 KPPPQNHRRIILLNTQNKID-GYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPPPDN 425

Query: 434 FPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWV 493
           FP+DYD+    PN+N+T GNGVY  +    +D+ILQNAN L    SEIHPWHLHGHDFWV
Sbjct: 426 FPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWV 485

Query: 494 LGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHM 553
           LGYGEGKFS   D KK NLKNPPLRNT VIFP+GWTA+RFV DNPGVW FHCHIEPHLHM
Sbjct: 486 LGYGEGKFSEK-DVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHM 545

Query: 554 GMGVVFAEGVHKVDRIPTKALACGETAKVLINN 575
           GMGV+FAEGVH V +IP +ALACG T K+L++N
Sbjct: 546 GMGVIFAEGVHLVKKIPKEALACGLTGKMLMSN 575

[Close]

BLAST of Clc03G15480 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 3.4e-218
Identity = 350/562 (62.28%), Postives = 424/562 (75.44%), Query Frame = 0


Query: 24  ISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTTVVEM 83
           I    I    A   VR Y WEVEY F  PDC E +VM +NGQFPGPTI A  GDT VV +
Sbjct: 6   IVAVAILAHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHL 65

Query: 84  INKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGETFIYRFVVDK----------- 143
            NKL+TEGLVIHWHGI Q G+PWADG A ++QCA +PGETF Y F VDK           
Sbjct: 66  TNKLATEGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYG 125

Query: 144 VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTSNPMRWIGDPE 203
           ++R+ GLYGSLI+D+ +GKKEP  YD + NLLLSDWWH+ +  QE+GL+S PMRWIG+ +
Sbjct: 126 MQRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQ 185

Query: 204 AILINGRGQTNCSIAAKY-NTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSAL 263
           +ILINGRGQ NCS+AA++ +TSL  C  K  +QCAP  LHV+PNKTYRIR+AS+T+L++L
Sbjct: 186 SILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASL 245

Query: 264 NFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPP 323
           NFA++ H+L+VVEADGNY+ PF T DIDIYSGE+YSVL+TTDQ  S+NY+++ GVR R P
Sbjct: 246 NFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKP 305

Query: 324 KTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRI 383
            TPPALT+LNY+    S+LP SPPPE P W+D+DRSKNF+ +I AAMGSP PP   + R+
Sbjct: 306 NTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFDERL 365

Query: 384 FLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIYNP 443
            LLNTQN I  GF KWAINNVSLA+P TPYL +++  L T F+++ PP+ +P DYDI  P
Sbjct: 366 ILLNTQNLIE-GFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTP 425

Query: 444 PPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSA 503
           P N N   GN    F     VDVILQNAN L    SEIHPWHLHGHDFWVLGYGEGKF  
Sbjct: 426 PRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKP 485

Query: 504 AVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGV 563
            VDEK  NLKNPPLRNTV ++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGVVFAEG+
Sbjct: 486 GVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGL 545

Query: 564 HKVDRIPTKALACGETAKVLIN 574
           +++ ++P +AL CG T + L+N
Sbjct: 546 NRIGKVPDEALGCGLTKQFLMN 566

[Close]

BLAST of Clc03G15480 vs. ExPASy TrEMBL
Match: Q9M5B5 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.3e-294
Identity = 476/588 (80.95%), Postives = 520/588 (88.44%), Query Frame = 0


Query: 3   KVANQPLFPMPFPVIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGI 62
           KVANQPLFPMPFP IF + SI  GFGITLS+A    RH+KWEVEYMF SPDC EN+VMGI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGE 122
           N QFPGPTIRANVGDT VVE+INKLSTE +VIHWHGILQKGTPWADGTASISQCA NPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHK 182
           +F Y+FVVDK           ++RA GLYGSLIVDLEEGKKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILH 242
           S+ KQEVGL+S+PMRWIG+P++ILINGRGQ+NCSI AKY  +++QC+L G E+CAPFIL 
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
           VKPNKTYRIRI STTSLSALNFAI NH+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFT 362
           TDQ  S+NYWVS+GVR R PKTPP LT+LNYLPNS SKLPISPPPE P W+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNT 422
           YRILAAMGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL LP TPYL AI+IG+N 
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHP 482
           TFDQNPPPET+PE+YDI+ PPPN NT TGNGVYRF+L EIVDV+LQNANTL DK SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NP++KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

[Close]

BLAST of Clc03G15480 vs. ExPASy TrEMBL
Match: A0A5D3CF11 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00020 PE=3 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.6e-293
Identity = 475/588 (80.78%), Postives = 518/588 (88.10%), Query Frame = 0


Query: 3   KVANQPLFPMPFPVIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGI 62
           K ANQPLFPMPFP IF + SI  GFGITLS+A    RH+KWEVEYMF SPDC EN+VMGI
Sbjct: 4   KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGE 122
           N QFPGPTIRANVGDT VVE+INKLSTE +VIHWHGILQKGTPWADGTASISQCA NPGE
Sbjct: 64  NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHK 182
           +F Y+FVVDK           ++RA GLYGSLIVDLEEGKKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILH 242
           S+ KQEVGL+S+PMRWIG+P++ILINGRGQ+NCSI AKY  +++QC+L G E CAPFIL 
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243

Query: 243 VKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
           VKPNKTYRIRI STTSLSALNFAI NH+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFT 362
           TDQ  S+NYWVS+GVR R PKTPP LT+LNYLPNS SKLPISPPPE P W+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNT 422
           YRILAAMGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL LP TPYL AI+IG+N 
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHP 482
           TFDQNPPPET+PE+YDI+ PPPN NT TGNGVYRF+L EIVDV+LQNANTL DK SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NP++KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

[Close]

BLAST of Clc03G15480 vs. ExPASy TrEMBL
Match: A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.5e-282
Identity = 465/591 (78.68%), Postives = 510/591 (86.29%), Query Frame = 0


Query: 1   MAKVANQPLFPMPFPVIFLVLSIISGFGITLSEA-VPIVRHYKWEVEYMFWSPDCVENVV 60
           MAKVA++P FP PF ++F VLSII GFGITLSEA  P +RHYKWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPF-LVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATN 120
           MGINGQFPGPTIRAN GD  VVE+ NKL TEG+VIHWHGILQ+GTPWADGTASISQCA N
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDK           ++R+ GLYGSLIVD +EG  EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH+S+ KQEVGL+S PMRWIG+P++ILINGRGQ +CSIAAKY   LKQCEL G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV P KTYRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ  SENYWVS+GVRAR PKTPP LT+LNYLPNSVSKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA++  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           L+T F+QNPPPETFP DYDI NPP N  T TGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           W FHCHIEPHLHMGMGV+FAEGVHKV  IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

[Close]

BLAST of Clc03G15480 vs. ExPASy TrEMBL
Match: Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 7.6e-282
Identity = 464/591 (78.51%), Postives = 509/591 (86.13%), Query Frame = 0


Query: 1   MAKVANQPLFPMPFPVIFLVLSIISGFGITLSEA-VPIVRHYKWEVEYMFWSPDCVENVV 60
           MAKVA++P FP PF ++F VLSII GFGITLSEA  P +RHYKWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPF-LVFFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATN 120
           MGINGQFPGPTIRAN GD  VVE+ NKL TEG+VIHWHGILQ+GTPWADGTASISQCA N
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDK           ++R+ GLYGSLIVD +EG  EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH+S+ KQEVGL+S PMRWIG+P++ILINGRGQ +CSIAAKY   LKQCEL G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV P K YRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ  SENYWVS+GVRAR PKTPP LT+LNYLPNSVSKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA++  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           L+T F+QNPPPETFP DYDI NPP N  T TGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           W FHCHIEPHLHMGMGV+FAEGVHKV  IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

[Close]

BLAST of Clc03G15480 vs. ExPASy TrEMBL
Match: A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 7.6e-282
Identity = 463/591 (78.34%), Postives = 512/591 (86.63%), Query Frame = 0


Query: 1   MAKVANQPLFPMPFPVIFLVLSIISGFGITLSEA-VPIVRHYKWEVEYMFWSPDCVENVV 60
           MAKVA++P FP PF + FLVLSII GFGITLSEA  P ++HYKW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPF-LSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATN 120
           MGING+FPGPTIRAN GD  VVE+ NKL TEG+VIHWHGILQ+GTPWADGTASISQCA N
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDK-----------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDK           ++R+ GLYGSLIVD  EG+ EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH+S+ KQEVGL+S PMRWIG+P++ILING+GQ +CSIAAKYN  LKQCEL G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV+P KTYRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ   ENYWVS+GVRAR PKTPP LT+LNYLPNS SKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA+++ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           LNT F+QNPPPETFP +YDI NPPPN  TTTGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPSKKP 580
           W FHCHIEPHLHMGMGVVFAEGVH V  IP KALACG TA  L+ N  + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587

[Close]

BLAST of Clc03G15480 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 767.3 bits (1980), Expect = 8.8e-222
Identity = 349/549 (63.57%), Postives = 425/549 (77.41%), Query Frame = 0


Query: 38  VRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWH 97
           VR Y WEVEY +WSPDC E  VM +NG+FPGPTI+A  GDT VV + NKL+TEGLVIHWH
Sbjct: 37  VREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLVIHWH 96

Query: 98  GILQKGTPWADGTASISQCATNPGETFIYRFVVDK-----------VRRAVGLYGSLIVD 157
           GI Q G+PWADG A ++QCA NPGETF Y F V+K           ++R+ GLYGSLIVD
Sbjct: 97  GIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGSLIVD 156

Query: 158 LEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSI 217
           + +GK E   YD + NLLLSDWWH++I  QE+GL+S PMRWIG+ ++ILINGRGQ NCS+
Sbjct: 157 VAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILINGRGQFNCSL 216

Query: 218 AAKY--NTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVE 277
           AA++  NTSL  C  K  +QCAP ILHV+PNKTYRIR++STT+L++LN A++ H+L+VVE
Sbjct: 217 AAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKLVVVE 276

Query: 278 ADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLP 337
           ADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+NY++SVGVR R P T  ALTILNY+ 
Sbjct: 277 ADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTILNYVT 336

Query: 338 NSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGF 397
              SKLP SPPP  P WDD++RSKNF+ +I +AMGSP PP K  +R+ LLNTQN I+ G+
Sbjct: 337 APASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLID-GY 396

Query: 398 VKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVY 457
            KWAINNVSL  P TPYL +++  L   F++  PP ++  DYDI NPPP  NTTTGNG+Y
Sbjct: 397 TKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIY 456

Query: 458 RFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPP 517
            F     VDVI+QNAN L+   SEIHPWHLHGHDFWVLGYG+GKF   +DEK  NLKNPP
Sbjct: 457 VFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPP 516

Query: 518 LRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALAC 574
           LRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGVVFAEG++++ ++P +AL C
Sbjct: 517 LRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALGC 576

[Close]

BLAST of Clc03G15480 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 707.2 bits (1824), Expect = 1.1e-203
Identity = 333/574 (58.01%), Postives = 423/574 (73.69%), Query Frame = 0


Query: 16  VIFLVLSIISGFGITLSEAVPIVRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANV 75
           +++ +L+++    +    A   V    WEVEY +W PDC E +VM INGQFPGPTI A  
Sbjct: 4   IVWWLLTVVV---VAFHSASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVA 63

Query: 76  GDTTVVEMINKLSTEGLVIHWHGILQKGTPWADGTASISQCATNPGETFIYRFVVDK--- 135
           GDT ++ ++NKLSTEG+VIHWHGI QKGTPWADG A ++QC  NPGETF Y+F+VDK   
Sbjct: 64  GDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGT 123

Query: 136 --------VRRAVGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTSNP 195
                   ++R+ GLYG LIV      KE   YD + NLLLSDWWH+SI  QE+ L+S P
Sbjct: 124 HFYHGHYGMQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSRP 183

Query: 196 MRWIGDPEAILINGRGQTNCSIAAKYNTSLKQ--CELKGDEQCAPFILHVKPNKTYRIRI 255
           MRWIG+P+++LINGRGQ NCS AA +N   ++  C  K ++QCAP  L V+PN+ YR+RI
Sbjct: 184 MRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRI 243

Query: 256 ASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWV 315
           ASTT+L++LN A++ HQL+VVEADGNYV PF  +DID+YSGE+YSVL+ T+   S+ YW+
Sbjct: 244 ASTTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWI 303

Query: 316 SVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPK 375
           SVGVR R PKTP ALT++NY+  + S+ P  PPP  P W+D DRSK+F+ +I AA G PK
Sbjct: 304 SVGVRGREPKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPK 363

Query: 376 PPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFD-QNPPPET 435
           PP KS+ ++ LLNTQN +   + KW+INNVSL++P TPYL +IR GL + +D ++P  + 
Sbjct: 364 PPEKSHDQLILLNTQN-LYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKL 423

Query: 436 FPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWV 495
             ++YDI  PPPN NTT G+G+Y F    +VDVILQNAN L+   SEIHPWH+HGHDFWV
Sbjct: 424 IMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWV 483

Query: 496 LGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHM 555
           LGYGEGKF   +DEK  NLKNPPLRNTVV++P+GWTAIRFV DNPGVW FHCHIEPHLHM
Sbjct: 484 LGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHM 543

Query: 556 GMGVVFAEGVHKVDR--IPTKALACGETAKVLIN 574
           GMGVVF EGV ++ +  IP +AL CG T K L+N
Sbjct: 544 GMGVVFVEGVDRIGKMEIPDEALGCGLTRKWLMN 569

[Close]

BLAST of Clc03G15480 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 696.4 bits (1796), Expect = 1.9e-200
Identity = 319/503 (63.42%), Postives = 386/503 (76.74%), Query Frame = 0


Query: 38  VRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWH 97
           VR Y WEVEY +WSPDC E  VM +NG+FPGPTI+A  GDT VV + NKL+TEGLVIHWH
Sbjct: 37  VREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLVIHWH 96

Query: 98  GILQKGTPWADGTASISQCATNPGETFIYRFVVDK-----------VRRAVGLYGSLIVD 157
           GI Q G+PWADG A ++QCA NPGETF Y F V+K           ++R+ GLYGSLIVD
Sbjct: 97  GIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGSLIVD 156

Query: 158 LEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSI 217
           + +GK E   YD + NLLLSDWWH++I  QE+GL+S PMRWIG+ ++ILINGRGQ NCS+
Sbjct: 157 VAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILINGRGQFNCSL 216

Query: 218 AAKY--NTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVE 277
           AA++  NTSL  C  K  +QCAP ILHV+PNKTYRIR++STT+L++LN A++ H+L+VVE
Sbjct: 217 AAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKLVVVE 276

Query: 278 ADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLP 337
           ADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+NY++SVGVR R P T  ALTILNY+ 
Sbjct: 277 ADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTILNYVT 336

Query: 338 NSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGF 397
              SKLP SPPP  P WDD++RSKNF+ +I +AMGSP PP K  +R+ LLNTQN I+ G+
Sbjct: 337 APASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLID-GY 396

Query: 398 VKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVY 457
            KWAINNVSL  P TPYL +++  L   F++  PP ++  DYDI NPPP  NTTTGNG+Y
Sbjct: 397 TKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIY 456

Query: 458 RFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPP 517
            F     VDVI+QNAN L+   SEIHPWHLHGHDFWVLGYG+GKF   +DEK  NLKNPP
Sbjct: 457 VFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPP 516

Query: 518 LRNTVVIFPYGWTAIRFVADNPG 528
           LRNT +++PYGWTAIRFV DNPG
Sbjct: 517 LRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of Clc03G15480 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 9.9e-157
Identity = 266/550 (48.36%), Postives = 351/550 (63.82%), Query Frame = 0


Query: 38  VRHYKWEVEYMFWSPDCVENVVMGINGQFPGPTIRANVGDTTVVEMINKLSTEGLVIHWH 97
           +R +KWEV+Y F SPDC E +V+ ING+FPGPTI+A  GDT VVE+ N   TE + +HWH
Sbjct: 35  IRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHWH 94

Query: 98  GILQKGTPWADGTASISQCATNPGETFIYRFVVDK-----------VRRAVGLYGSLIVD 157
           GI Q GTPW DG   ++QC   PGE FIY+FVVD+           ++R  GL G + V 
Sbjct: 95  GIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQVS 154

Query: 158 LEEGKKEPFHYDEDINLLLSDWWHKSIQKQEVGLTSNPMRWIGDPEAILINGRGQTNCSI 217
               + EPF YD D N LL+DW+HKS+ ++  GL S P +W+G+P++++I GRG+ NCS 
Sbjct: 155 PPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKWVGEPQSLMIQGRGRFNCSN 214

Query: 218 AAKYNTSLKQ--CELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVE 277
                 SL    C +  +  C+ FIL V P KTYR+RI S T+LSAL+F I  H L VVE
Sbjct: 215 NLTTPPSLVSGVCNV-SNADCSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVVE 274

Query: 278 ADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLP 337
           ADG+YV+PF   ++ +YSGE+YSVL+  DQ    NYW++  + +RP  TPPA  +LNY P
Sbjct: 275 ADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYYP 334

Query: 338 NSVSKLPISPPPE--IPNWDDYDRSKNFTYRILAAMGSPKP-PVKSNRRIFLLNTQNHIN 397
           N   + P +      +P W+D       +  I A  G     P  S++ I LLNTQN +N
Sbjct: 335 NHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQNEVN 394

Query: 398 GGFVKWAINNVSLALPPTPYLAAIRIGLNTTFD-QNPPPETF-PEDYDIYNPPPNTNTTT 457
            G+ +W++NNVS   P TPYL A++  L   FD +   PE +   +YDI+  P N N TT
Sbjct: 395 -GYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNANATT 454

Query: 458 GNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLN 517
            +G+YR      VDVILQNANT+    SE HPWHLHGHDFWVLGYGEGKF+ + D K+ N
Sbjct: 455 SDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPKRYN 514

Query: 518 LKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDRIPT 570
             +P  +NTV + P+GWTA+RF ADNPGVW FHCHIE H  MGMG+VF  G+ KV  +P+
Sbjct: 515 RVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSSLPS 574

[Close]

BLAST of Clc03G15480 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 542.0 bits (1395), Expect = 6.0e-154
Identity = 248/384 (64.58%), Postives = 303/384 (78.91%), Query Frame = 0


Query: 192 EAILINGRGQTNCSIAAKY--NTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLS 251
           ++ILINGRGQ NCS+AA++  NTSL  C  K  +QCAP ILHV+PNKTYRIR++STT+L+
Sbjct: 11  KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70

Query: 252 ALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRAR 311
           +LN A++ H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+NY++SVGVR R
Sbjct: 71  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130

Query: 312 PPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNR 371
            P T  ALTILNY+    SKLP SPPP  P WDD++RSKNF+ +I +AMGSP PP K  +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190

Query: 372 RIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIY 431
           R+ LLNTQN I+ G+ KWAINNVSL  P TPYL +++  L   F++  PP ++  DYDI 
Sbjct: 191 RLILLNTQNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 250

Query: 432 NPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKF 491
           NPPP  NTTTGNG+Y F     VDVI+QNAN L+   SEIHPWHLHGHDFWVLGYG+GKF
Sbjct: 251 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 310

Query: 492 SAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAE 551
              +DEK  NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGVVFAE
Sbjct: 311 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 370

Query: 552 GVHKVDRIPTKALACGETAKVLIN 574
           G++++ ++P +AL CG T + L+N
Sbjct: 371 GLNRIGKVPDEALGCGLTKQFLMN 393

The following BLAST results are available for this feature:

BLAST of Clc03G15480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880052.1	9.8e-300	82.37	L-ascorbate oxidase-like [Benincasa hispida]	[more]
XP_038878978.1	1.5e-295	82.26	L-ascorbate oxidase [Benincasa hispida]	[more]
NP_001284440.1	4.7e-294	80.95	L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucum...	[more]
TYK09892.1	1.2e-292	80.78	L-ascorbate oxidase-like [Cucumis melo var. makuwa]	[more]
KAA0055636.1	3.2e-282	78.68	L-ascorbate oxidase [Cucumis melo var. makuwa]	[more]

BLAST of Clc03G15480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14133	2.1e-284	78.34	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P24792	4.6e-268	76.88	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	1.3e-265	77.78	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
Q40588	2.8e-225	63.87	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]
M4DUF2	3.4e-218	62.28	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]

BLAST of Clc03G15480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q9M5B5	2.3e-294	80.95	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1	[more]
A0A5D3CF11	5.6e-293	80.78	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G0...	[more]
A0A5A7UMY3	1.5e-282	78.68	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G...	[more]
Q9M5B4	7.6e-282	78.51	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1	[more]
A0A0A0LXB9	7.6e-282	78.34	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1	[more]

BLAST of Clc03G15480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G21105.1	8.8e-222	63.57	Plant L-ascorbate oxidase	[more]
AT5G21100.1	1.1e-203	58.01	Plant L-ascorbate oxidase	[more]
AT5G21105.3	1.9e-200	63.42	Plant L-ascorbate oxidase	[more]
AT4G39830.1	9.9e-157	48.36	Cupredoxin superfamily protein	[more]
AT5G21105.2	6.0e-154	64.58	Plant L-ascorbate oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 45..132 e-value: 4.5E-27 score: 94.4
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 159..360 e-value: 2.0E-64 score: 219.4
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 361..578 e-value: 1.2E-72 score: 246.1
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 29..158 e-value: 6.4E-33 score: 115.8
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 154..359
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 38..171
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 363..564
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 435..550 e-value: 9.1E-35 score: 119.4
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 161..323 e-value: 2.0E-46 score: 158.0
IPR017760	L-ascorbate oxidase, plants	TIGRFAM	TIGR03388	TIGR03388	coord: 38..567 e-value: 3.1E-280 score: 928.4
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 33..568
None	No IPR available	PANTHER	PTHR11709:SF394	PLANT L-ASCORBATE OXIDASE	coord: 33..568
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 527..547
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 532..543
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 367..558 e-value: 1.42265E-83 score: 255.808

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc03G15480.2	Clc03G15480.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity
molecular_function	GO:0016491	oxidoreductase activity