Clc03G11400 (gene) Watermelon (cordophanus) v2

Overview
NameClc03G11400
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptiontRNA pseudouridine synthase B
LocationClcChr03: 14952214 .. 14955379 (-)
RNA-Seq ExpressionClc03G11400
SyntenyClc03G11400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAATCACCTGCTCTGTTTTACGCCTCCCGTTTCTTCATCTGCTCTACACTCATGGCTTCTTCCAAATCACTCACTCTCTTCCTTCCACTCTCAAACCTCAACCATCTCCACGGGTTTGCCTTTCGCAATTCACTCTCTTCCTTATCGCTTTGCAGATTCTCCACCACTTCAACCCCATTCCCCCTACAATACGAAATGATCATCAACCGACCTTATCCTCTGCCGCCCCATCAGAATGGACGAACACCCGCCAGAATCAGCTCCGACAACTCTCCCGAGCTCCACTCTTCCGAGGATTCCACTTCCGAATTGGGGTTCGAGAGCTGGATGGATCGGAAGCTTATATCCGAGAGTGAAGCTGTGTCCGGTAAGGAAGGCGTGGTCATGGATAAGGCCATGAGGAAATACTATAATAAAAGAAGGAAGAGGATGTATGGGTCTAATGAAGATGAAGATAATAAGACGCAGGACGAGGGATTTGCTTAAAGCCTGAGGTGGTTGAGTTTAACACATTGCATAAGAGAGAGGAGGAATTGTTCTTTTATGATGCTTTTGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGTATCAGTTAGAGAAAATATATTTTCCTGATGAAGGTCTTGACAAGGCTTTTTCGGGACCTGGAGAGTCAAATGTGGAAGTCAATGAACAGACAAAGGCAAGGAAGGGGGTGAAAAAGGCAGTGGGGGTTAAGCCCGAGACGAAAGTAGAGGTCCCCAATGGCATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAAACCAGAGAAGGATAATAAGGGGTCGGTGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCAGCTAAAGATTCTGAAATTGGTCAGATGGATCCATATCTTTTAACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTCTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGGTGGACTTCATTTACGGTATGTGGAAAACTTTGGCGCCTAGTCAAAGTGAAAAAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCGATGAAGTTGGTAGACAGATATCAAGGTATGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTACCTCCCATGTTTTCTGCCATTAAAGTTGGAGGTGAGAAGATGTACGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTCTCCCCAAGACGGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAGATGACAGGCAAAGCTTGATTTTTCGAGTGACATGCTCTAAAGGAACATACATACGGTCACCTTGTGCAGATCTTGGGAAGGTTCTTGGCAACTATTTCAAAAAAAATACCACCTATTGCAATTACCCTTTCTTAAATTCTTGCTTTGAGCAAGGAAAAAATTTAGGCCATGAATATATTAGGGGCTTGAGAAAGTTGGCGTTGTATTTTATACACTTGACCTCAGCACCTGAATTATTCATCTAGTTCTTCACATCTCACTCATTTATTCATCTAGTTCTCCACATCTCACTTCATCCAACTTGAGGCTGTCTGGTGCTTGTATCACCCTTATCCTGCGAACTAATTTGTGATTTGAAATTATCGATTGAACTTGCTACCGTTTTTGTGAGTGTGTGCAATGTTTCAAAAGGTTTTTGAGGCATAGTCCATGATGCTCACCTGAGGTTGAGGCTCGAACTTTTAGCCTCAAAGACTTGCACTTTCTTTAGATCATAAGTGGCTGTGGCTTCTTTAGGCTTCTTATAGAGAGGCCTGTGTTCATGAGAATGCATCCAGGTTTGCAGCCTTTACCTCATGAAATAGCCAGAGGAATATATTTAACTTGTTAACTAAAATTATATTAAGAAAATTTGCTTAGACTTTAATGTTTTAATATACCATGAGTTTCGGTAACTTAGGCCCTTACTAGTTTTACCATCAAGGTAAAAAAAGAACTTTGAAGCAAAGTGAAGGATGAGTTGAGTCTATTATGATATTTATTTAAGTGACATTTGACATGCAGGAGTTTCTTTTCCTCCATTTTTGTACTTCTTGATAATCAATTTAATTTAACAATACTTGATCTTGGTAGGAGTGGGAGAGGGAATGATATCTAATATGATAGGGTTTGGGTTAGTTACTTTACCTCTATATTACTTGCTGACTTAGCTTAAAAAATGAAATTATTGATAAATGAAATTAGGGGGAAATGTTAGTTCTTTCCAGTTAAGAGGCCATTTCTGATGCCATTGCATTTCATGCTTGTTAACATCAGCTGATGGTGATGAGTATGAGTATATTTAGAGGATCCATATTTCATAATCTGATAATCTAAGTCCTGACTATACGCTCTCGTGCTGAGGACATATTTTAGTTTTTGATATTTTCTTTTATTGCAACACATCATTTTGCTTGGATTCTCTGCTAATATAAGTTGTACCTTCTGACAACTATTCCCCAGCTCTTGCTGCAAAAAGGAACAGGTATCAGAGGCAGAAATCCTTAATGCTGTTGGAAATGGAAAGTCAACATGCCAAGGGATTATCACCTTTCACTCCCCTCAACTGAATTATGCTGCAAAAAACAGGAAAAAAGTCCTTGGGAGAGTTTTGAAACCTGCAACAGACCTTTAGAACACTAACTTAAATACAACCGTACAGGATGTTCGAACATAAAATAGTCATCGGCTTTGCTGCTGAAATGAGACTGAAGAATCTGGCTTTCATGAGAGCTTCATTCAAGTAACAAAGCAATATATCACAGAGAGCAATTACACCTTGATCAATGGAACAGGGACAATTTTTCTTTGTAGCATTTTGATGGTATGATCCAATAGAGCATTTGATTGGTGTACATTTGGAATGGCATAGATCATTCTATGTAATGAGATGTCTTCGTAATATGTAGTTCTGTTCCAAAAGTGTATATACAGAGTGTTTTTGGTGAATAGTAGTCAGTTTTATCCACTACTTGTACCAGTT

mRNA sequence

ATGGCGAAATCACCTGCTCTGTTTTACGCCTCCCGTTTCTTCATCTGCTCTACACTCATGGCTTCTTCCAAATCACTCACTCTCTTCCTTCCACTCTCAAACCTCAACCATCTCCACGGGTTTGCCTTTCGCAATTCACTCTCTTCCTTATCGCTTTGCAGATTCTCCACCACTTCAACCCCATTCCCCCTACAATACGAAATGATCATCAACCGACCTTATCCTCTGCCGCCCCATCAGAATGGACGAACACCCGCCAGAATCAGCTCCGACAACTCTCCCGAGCTCCACTCTTCCGAGGATTCCACTTCCGAATTGGGGTTCGAGAGCTGGATGGATCGGAAGCTTATATCCGAGAGTGAAGCTGTGTCCGGACGAGGGATTTGCTTAAAGCCTGAGGTGGTTGAGTTTAACACATTGCATAAGAGAGAGGAGGAATTGTTCTTTTATGATGCTTTTGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGTATCAGTTAGAGAAAATATATTTTCCTGATGAAGGTCTTGACAAGGCTTTTTCGGGACCTGGAGAGTCAAATGTGGAAGTCAATGAACAGACAAAGGCAAGGAAGGGGGTGAAAAAGGCAGTGGGGGTTAAGCCCGAGACGAAAGTAGAGGTCCCCAATGGCATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAAACCAGAGAAGGATAATAAGGGGTCGGTGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCAGCTAAAGATTCTGAAATTGGTCAGATGGATCCATATCTTTTAACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTCTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGGTGGACTTCATTTACGGTATGTGGAAAACTTTGGCGCCTAGTCAAAGTGAAAAAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCGATGAAGTTGGTAGACAGATATCAAGGTATGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTACCTCCCATGTTTTCTGCCATTAAAGTTGGAGGTGAGAAGATGTACGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTCTCCCCAAGACGGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAGATGACAGGCAAAGCTTGATTTTTCGAGTGACATGCTCTAAAGGAACATACATACGGTCACCTTGTGCAGATCTTGGGAAGGCTTCTTATAGAGAGGCCTGTGTTCATGAGAATGCATCCAGCTCTTGCTGCAAAAAGGAACAGGTATCAGAGGCAGAAATCCTTAATGCTGTTGGAAATGGAAAGTCAACATGCCAAGGGATTATCACCTTTCACTCCCCTCAACTGAATTATGCTGCAAAAAACAGGAAAAAAGTCCTTGGGAGAGTTTTGAAACCTGCAACAGACCTTTAGAACACTAACTTAAATACAACCGTACAGGATGTTCGAACATAAAATAGTCATCGGCTTTGCTGCTGAAATGAGACTGAAGAATCTGGCTTTCATGAGAGCTTCATTCAAGTAACAAAGCAATATATCACAGAGAGCAATTACACCTTGATCAATGGAACAGGGACAATTTTTCTTTGTAGCATTTTGATGGTATGATCCAATAGAGCATTTGATTGGTGTACATTTGGAATGGCATAGATCATTCTATGTAATGAGATGTCTTCGTAATATGTAGTTCTGTTCCAAAAGTGTATATACAGAGTGTTTTTGGTGAATAGTAGTCAGTTTTATCCACTACTTGTACCAGTT

Coding sequence (CDS)

ATGGCGAAATCACCTGCTCTGTTTTACGCCTCCCGTTTCTTCATCTGCTCTACACTCATGGCTTCTTCCAAATCACTCACTCTCTTCCTTCCACTCTCAAACCTCAACCATCTCCACGGGTTTGCCTTTCGCAATTCACTCTCTTCCTTATCGCTTTGCAGATTCTCCACCACTTCAACCCCATTCCCCCTACAATACGAAATGATCATCAACCGACCTTATCCTCTGCCGCCCCATCAGAATGGACGAACACCCGCCAGAATCAGCTCCGACAACTCTCCCGAGCTCCACTCTTCCGAGGATTCCACTTCCGAATTGGGGTTCGAGAGCTGGATGGATCGGAAGCTTATATCCGAGAGTGAAGCTGTGTCCGGACGAGGGATTTGCTTAAAGCCTGAGGTGGTTGAGTTTAACACATTGCATAAGAGAGAGGAGGAATTGTTCTTTTATGATGCTTTTGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGTATCAGTTAGAGAAAATATATTTTCCTGATGAAGGTCTTGACAAGGCTTTTTCGGGACCTGGAGAGTCAAATGTGGAAGTCAATGAACAGACAAAGGCAAGGAAGGGGGTGAAAAAGGCAGTGGGGGTTAAGCCCGAGACGAAAGTAGAGGTCCCCAATGGCATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAAACCAGAGAAGGATAATAAGGGGTCGGTGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCAGCTAAAGATTCTGAAATTGGTCAGATGGATCCATATCTTTTAACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTCTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGGTGGACTTCATTTACGGTATGTGGAAAACTTTGGCGCCTAGTCAAAGTGAAAAAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCGATGAAGTTGGTAGACAGATATCAAGGTATGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTACCTCCCATGTTTTCTGCCATTAAAGTTGGAGGTGAGAAGATGTACGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTCTCCCCAAGACGGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAGATGACAGGCAAAGCTTGATTTTTCGAGTGACATGCTCTAAAGGAACATACATACGGTCACCTTGTGCAGATCTTGGGAAGGCTTCTTATAGAGAGGCCTGTGTTCATGAGAATGCATCCAGCTCTTGCTGCAAAAAGGAACAGGTATCAGAGGCAGAAATCCTTAATGCTGTTGGAAATGGAAAGTCAACATGCCAAGGGATTATCACCTTTCACTCCCCTCAACTGAATTATGCTGCAAAAAACAGGAAAAAAGTCCTTGGGAGAGTTTTGAAACCTGCAACAGACCTTTAG

Protein sequence

MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTSTPFPLQYEMIINRPYPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISESEAVSGRGICLKPEVVEFNTLHKREEELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVKKAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKAMKLVDRYQGMSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLDDRQSLIFRVTCSKGTYIRSPCADLGKASYREACVHENASSSCCKKEQVSEAEILNAVGNGKSTCQGIITFHSPQLNYAAKNRKKVLGRVLKPATDL
Homology
BLAST of Clc03G11400 vs. NCBI nr
Match: KAA0037597.1 (TruB_N domain-containing protein [Cucumis melo var. makuwa] >TYK09336.1 TruB_N domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 798.5 bits (2061), Expect = 3.5e-227
Identity = 418/518 (80.69%), Postives = 439/518 (84.75%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MAKSPALF+AS FFI STLM SSKSL LFLPLSNLNH H FAFRNSLS LSL RF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQYE+IINRP YP PPHQN RTPARISSDN PEL+SSED +SELGF+SW+DRKLISE
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           S  VSG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLEK YFPD+GLDKAF GPGESNVEVNEQTK R+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KAV VKPET VEV NGM+DKG+VFFDEGKPEK+NKGSV DV EKKVEEFFKCLKK PAKD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           S+IGQ +PYLLTRH ELPA+WDSP GTVVLLNKPKGWTSFTVCGKL RLVKV+KVGHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KLVDRYQGM   YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

BLAST of Clc03G11400 vs. NCBI nr
Match: XP_008458830.1 (PREDICTED: uncharacterized protein LOC103498118 [Cucumis melo])

HSP 1 Score: 798.5 bits (2061), Expect = 3.5e-227
Identity = 418/518 (80.69%), Postives = 439/518 (84.75%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MAKSPALF+AS FFI STLM SSKSL LFLPLSNLNH H FAFRNSLS LSL RF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQYE+IINRP YP PPHQN RTPARISSDN PEL+SSED +SELGF+SW+DRKLISE
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           S  VSG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLEK YFPD+GLDKAF GPGESNVEVNEQTK R+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KAV VKPET VEV NGM+DKG+VFFDEGKPEK+NKGSV DV EKKVEEFFKCLKK PAKD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           S+IGQ +PYLLTRH ELPA+WDSP GTVVLLNKPKGWTSFTVCGKL RLVKV+KVGHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KLVDRYQGM   YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

BLAST of Clc03G11400 vs. NCBI nr
Match: XP_038891059.1 (uncharacterized protein LOC120080471 [Benincasa hispida])

HSP 1 Score: 797.3 bits (2058), Expect = 7.9e-227
Identity = 417/518 (80.50%), Postives = 433/518 (83.59%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           M KS ALF+AS FFI ST M SS +LTLFL  SNLNHLH FAFRNSLS LS  R STTST
Sbjct: 1   MVKSSALFHASNFFIRSTFMPSSITLTLFLLPSNLNHLHRFAFRNSLSPLSPRRLSTTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQYEMIINRP YP PPHQN RTP RI SDNSPE  SSED TSE+GFESW+DRKLISE
Sbjct: 61  PFPLQYEMIINRPSYPPPPHQNRRTPTRIRSDNSPEFDSSEDPTSEMGFESWVDRKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           SE +SG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SETISGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFFYDAFAYPWEKDKHYKMVYQLEK YFPDEGLDKAF GPGESNVEVNEQTK RKGVK
Sbjct: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           K VGV+PE KV+V +GM+DKGLVFFDEGKPE +NKGSVKDV EKKVEEFFKCLKKVPAKD
Sbjct: 241 KGVGVEPEMKVKVTHGMDDKGLVFFDEGKPENENKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           SEIG  +PYLLTRHTELPAKWDSP GTVVLLNKPKGWTSFTVCGKL RLVKVKKVGHAGT
Sbjct: 301 SEIGPTEPYLLTRHTELPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KLV+RYQGM  SYSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
            DIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 GDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

BLAST of Clc03G11400 vs. NCBI nr
Match: XP_022938741.1 (uncharacterized protein LOC111444875 [Cucurbita moschata] >KAG7016215.1 truB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 789.3 bits (2037), Expect = 2.1e-224
Identity = 416/518 (80.31%), Postives = 433/518 (83.59%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MA SPAL +AS FFI STLMASSKSL  F PLSNLNHLH   F NSLS LSL R STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLH--RFPNSLSLLSLRRSSTTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQY+MII+RP YP  P QN RTPARIS DNSPEL SSED  SELGFESW+D+KLISE
Sbjct: 61  PFPLQYDMIIDRPIYPPMPPQNRRTPARISPDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           SE+ SG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRKRMYGSDSDEEKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFFYD FAYPWEKDKHYKMVYQLEK YFPDEGLDKAF GPGESNVEVNEQTK RKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KAVGVKPETKVEV NG+++KGLVFFD G+PEKDNKGSVKDV EKKVEEFFKCLKKVPAKD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           SEIGQM PYLLTRHTELPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGT
Sbjct: 301 SEIGQMGPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKA ASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISI+KFDIERSLD
Sbjct: 421 DDIKKAVASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

BLAST of Clc03G11400 vs. NCBI nr
Match: KAG6578676.1 (hypothetical protein SDJN03_23124, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 788.1 bits (2034), Expect = 4.8e-224
Identity = 415/518 (80.12%), Postives = 433/518 (83.59%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MA SPAL +AS FFI STLMASSKSL  F PLSNLNHLH   F NSLS LSL R STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLH--RFPNSLSLLSLRRSSTTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQY+MII+RP YP  P QN RTPARIS DNSPEL SSED  SELGFESW+D+KLISE
Sbjct: 61  PFPLQYDMIIDRPIYPPMPPQNRRTPARISPDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           SE+ SG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRKRMYGSDSDEEKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFFYD FAYPWEKDKHYKMVYQLEK YFPDEGLDKAF GPGESNVEVNEQTK RKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KAVGVKPETKVEV NG+++KGLVFFD G+P+KDNKGSVKDV EKKVEEFFKCLKKVPAKD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPDKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           SEIGQM PYLLTRHTELPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGT
Sbjct: 301 SEIGQMGPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKA ASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISI+KFDIERSLD
Sbjct: 421 DDIKKAVASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

BLAST of Clc03G11400 vs. ExPASy Swiss-Prot
Match: Q9RB36 (tRNA pseudouridine synthase B OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=truB PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.9e-38
Identity = 89/219 (40.64%), Postives = 130/219 (59.36%), Query Frame = 0

Query: 290 GTVVLLNKPKGWTSFTVCGKLWRLV-------KVKKVGHAGTLDPMATGLLIVCVGKAMK 349
           G V+L++KP  W+SF    KL  L+       K  K+GHAGTLDP+ATGLL++C GK  K
Sbjct: 9   GQVLLIDKPLKWSSFQAVNKLKYLLINKVGLPKKFKIGHAGTLDPLATGLLLICTGKFTK 68

Query: 350 LVDRYQGMS--YSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVP 409
            +   QG +  Y+G F +G  T ++D ++ + Q  P EHI +  I +    F GEI Q P
Sbjct: 69  KISELQGQAKEYTGTFYIGATTPSYDLETEIDQTFPTEHINEVLIHETVKQFLGEIDQKP 128

Query: 410 PMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLDDRQSLIFRVTCSKGTYIR 469
           P+FSAIK  G ++YE AR GESIE+  R+ +I +F+I R       + FRV CSKGTYIR
Sbjct: 129 PIFSAIKKDGVRLYEHARAGESIEIESRKTTIHEFEITRIA--LPEIDFRVVCSKGTYIR 188

Query: 470 SPCADLGKASYREACVHENASSSCCKKEQVSEAEILNAV 500
           S   D GKA      ++  +  +  ++ ++ + ++ NA+
Sbjct: 189 SLAYDFGKA------MNSGSHLTVLRRTKIGDYDVKNAI 219

BLAST of Clc03G11400 vs. ExPASy Swiss-Prot
Match: Q5LJ29 (tRNA pseudouridine synthase B OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=truB PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.6e-36
Identity = 83/189 (43.92%), Postives = 118/189 (62.43%), Query Frame = 0

Query: 290 GTVVLLNKPKGWTSFTVCG----KLWRLVKVK--KVGHAGTLDPMATGLLIVCVGKAMKL 349
           G V+  NKP GWTSF V G     + R +KVK  KVGHAGTLDP+ATG++IVC GKA K 
Sbjct: 6   GEVLYFNKPLGWTSFKVVGHARYHMCRRMKVKKLKVGHAGTLDPLATGVMIVCTGKATKR 65

Query: 350 VD--RYQGMSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPP 409
           ++  +Y    Y    +LG  T ++D +  +    P EHI  + ++K   +F GEI Q+PP
Sbjct: 66  IEEFQYHTKEYVATIQLGATTPSYDLEHEIDATYPTEHITRELVEKTLKTFVGEIQQIPP 125

Query: 410 MFSAIKVGGEKMYEKARRGESIELSPRRISIFKFD-IERSLDDRQSLIFRVTCSKGTYIR 469
            FSA KV G + Y+ AR+G+ +EL P+ + I + + +E +L +   +  RV CSKGTYIR
Sbjct: 126 AFSACKVDGARAYDLARKGQEVELKPKLLVIDEIELLECNLPE---IKIRVVCSKGTYIR 185

BLAST of Clc03G11400 vs. ExPASy Swiss-Prot
Match: Q650L8 (tRNA pseudouridine synthase B OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=truB PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.6e-36
Identity = 83/189 (43.92%), Postives = 118/189 (62.43%), Query Frame = 0

Query: 290 GTVVLLNKPKGWTSFTVCG----KLWRLVKVK--KVGHAGTLDPMATGLLIVCVGKAMKL 349
           G V+  NKP GWTSF V G     + R +KVK  KVGHAGTLDP+ATG++IVC GKA K 
Sbjct: 6   GEVLYFNKPLGWTSFKVVGHARYHMCRRMKVKKLKVGHAGTLDPLATGVMIVCTGKATKR 65

Query: 350 VD--RYQGMSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPP 409
           ++  +Y    Y    +LG  T ++D +  +    P EHI  + ++K   +F GEI Q+PP
Sbjct: 66  IEEFQYHTKEYVATIQLGATTPSYDLEHEIDATYPTEHITRELVEKTLKTFVGEIQQIPP 125

Query: 410 MFSAIKVGGEKMYEKARRGESIELSPRRISIFKFD-IERSLDDRQSLIFRVTCSKGTYIR 469
            FSA KV G + Y+ AR+G+ +EL P+ + I + + +E +L +   +  RV CSKGTYIR
Sbjct: 126 AFSACKVDGARAYDLARKGQEVELKPKLLVIDEIELLECNLPE---IKIRVVCSKGTYIR 185

BLAST of Clc03G11400 vs. ExPASy Swiss-Prot
Match: A6LEL7 (tRNA pseudouridine synthase B OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152) OX=435591 GN=truB PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.6e-36
Identity = 83/192 (43.23%), Postives = 118/192 (61.46%), Query Frame = 0

Query: 286 DSPSGTVVLLNKPKGWTSFTVCG----KLWRLVKVK--KVGHAGTLDPMATGLLIVCVGK 345
           D  +G ++  NKP  WTSF +      KL R +KVK  KVGHAGTLDP+ATG++IVC G+
Sbjct: 2   DFIAGEILYFNKPLTWTSFDLVNKFRYKLSRKLKVKKIKVGHAGTLDPLATGVMIVCTGR 61

Query: 346 AMKLVD--RYQGMSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIW 405
           A K +D  +YQ   Y    +LGE T ++D +  +    P EHI  + +++   +F G I 
Sbjct: 62  ATKRIDEFQYQTKEYIATLKLGETTPSFDLEKEIDAVYPTEHITRELVEEVLKTFVGTIQ 121

Query: 406 QVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLDDRQSLIFRVTCSKGT 465
           Q+PP+FSA KV G++ YE AR+GE +EL  + + I + ++     D   +  RV CSKGT
Sbjct: 122 QIPPVFSACKVEGKRAYELARKGEEVELKSKTLVIDELELLEC--DLPVIKIRVVCSKGT 181

Query: 466 YIRSPCADLGKA 470
           YIR+   D+GKA
Sbjct: 182 YIRALARDIGKA 191

BLAST of Clc03G11400 vs. ExPASy Swiss-Prot
Match: Q47RU8 (tRNA pseudouridine synthase B OS=Thermobifida fusca (strain YX) OX=269800 GN=truB PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.6e-36
Identity = 80/185 (43.24%), Postives = 116/185 (62.70%), Query Frame = 0

Query: 292 VVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKAMKLVDRYQGMS 351
           VV+++KP GWTS  V  ++ RL   ++VGHAGTLDPMATG+L+V VGKA +L+  Y  ++
Sbjct: 5   VVIVDKPAGWTSHDVVARVRRLAGTRRVGHAGTLDPMATGVLVVGVGKATRLLG-YLALT 64

Query: 352 ---YSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKV 411
              Y    RLG++T+T DA+  +++R P +HI +  +     +  G I QVPP  SA+KV
Sbjct: 65  EKVYEATIRLGQSTTTDDAEGELLERRPADHIDEAAVHAGTRALTGVIHQVPPQVSAVKV 124

Query: 412 GGEKMYEKARRGESIELSPRRISIFKFDIE--RSLDDRQSLIF----RVTCSKGTYIRSP 468
            G++ Y +AR GE++EL  R +++ +F +   R +D           RVTCS GTYIRS 
Sbjct: 125 RGQRAYRRARAGETVELKAREVTVHEFTVTGFRRVDSPDGAFVDVDARVTCSSGTYIRSL 184

BLAST of Clc03G11400 vs. ExPASy TrEMBL
Match: A0A5A7T7S0 (TruB_N domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00150 PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.7e-227
Identity = 418/518 (80.69%), Postives = 439/518 (84.75%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MAKSPALF+AS FFI STLM SSKSL LFLPLSNLNH H FAFRNSLS LSL RF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQYE+IINRP YP PPHQN RTPARISSDN PEL+SSED +SELGF+SW+DRKLISE
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           S  VSG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLEK YFPD+GLDKAF GPGESNVEVNEQTK R+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KAV VKPET VEV NGM+DKG+VFFDEGKPEK+NKGSV DV EKKVEEFFKCLKK PAKD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           S+IGQ +PYLLTRH ELPA+WDSP GTVVLLNKPKGWTSFTVCGKL RLVKV+KVGHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KLVDRYQGM   YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

BLAST of Clc03G11400 vs. ExPASy TrEMBL
Match: A0A1S3C8B0 (uncharacterized protein LOC103498118 OS=Cucumis melo OX=3656 GN=LOC103498118 PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.7e-227
Identity = 418/518 (80.69%), Postives = 439/518 (84.75%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MAKSPALF+AS FFI STLM SSKSL LFLPLSNLNH H FAFRNSLS LSL RF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQYE+IINRP YP PPHQN RTPARISSDN PEL+SSED +SELGF+SW+DRKLISE
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           S  VSG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLEK YFPD+GLDKAF GPGESNVEVNEQTK R+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KAV VKPET VEV NGM+DKG+VFFDEGKPEK+NKGSV DV EKKVEEFFKCLKK PAKD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           S+IGQ +PYLLTRH ELPA+WDSP GTVVLLNKPKGWTSFTVCGKL RLVKV+KVGHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KLVDRYQGM   YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

BLAST of Clc03G11400 vs. ExPASy TrEMBL
Match: A0A6J1FDZ7 (uncharacterized protein LOC111444875 OS=Cucurbita moschata OX=3662 GN=LOC111444875 PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 1.0e-224
Identity = 416/518 (80.31%), Postives = 433/518 (83.59%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MA SPAL +AS FFI STLMASSKSL  F PLSNLNHLH   F NSLS LSL R STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLH--RFPNSLSLLSLRRSSTTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQY+MII+RP YP  P QN RTPARIS DNSPEL SSED  SELGFESW+D+KLISE
Sbjct: 61  PFPLQYDMIIDRPIYPPMPPQNRRTPARISPDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           SE+ SG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRKRMYGSDSDEEKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFFYD FAYPWEKDKHYKMVYQLEK YFPDEGLDKAF GPGESNVEVNEQTK RKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KAVGVKPETKVEV NG+++KGLVFFD G+PEKDNKGSVKDV EKKVEEFFKCLKKVPAKD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           SEIGQM PYLLTRHTELPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGT
Sbjct: 301 SEIGQMGPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKA ASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISI+KFDIERSLD
Sbjct: 421 DDIKKAVASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

BLAST of Clc03G11400 vs. ExPASy TrEMBL
Match: A0A6J1JSH8 (uncharacterized protein LOC111489372 OS=Cucurbita maxima OX=3661 GN=LOC111489372 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.3e-224
Identity = 415/518 (80.12%), Postives = 432/518 (83.40%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MA SPAL +AS FFI STLMASSKSL  F PLSNLNHLH   F NSLS LSL R STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLH--RFPNSLSLLSLRRSSTTST 60

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
           PFPLQY+MIIN P YP  P QN RTP RISSDNSPEL SSED  SELGFESW+D+KLISE
Sbjct: 61  PFPLQYDMIINHPIYPPMPPQNRRTPPRISSDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
           SE+ SG+                                    + LKPEVVEFNTLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRRRMYGSDSDEDKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFFYD FAYPWEKDKHYKMVYQLEK YFPDEGLDKAF GPGESNVEVNEQTK RKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KAVGVKPETKVEV NG+++KGLVFFD G+PEKDNKGSVKDV EKKVEEFFKCLKKVPAKD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           SEIGQ +PYLLTRHTELPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGT
Sbjct: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISI+KFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

BLAST of Clc03G11400 vs. ExPASy TrEMBL
Match: A0A0A0KP03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G522940 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.3e-224
Identity = 413/518 (79.73%), Postives = 437/518 (84.36%), Query Frame = 0

Query: 1   MAKSPALFYASRFFICSTLMASSKSLTLFLPLSNLNHLHGFAFRNSLSSLSLCRFSTTST 60
           MAKSPALF+AS FFI STLM SSKSL+LFLPLSNLNHLH FAFRNSLS LSL  FSTT T
Sbjct: 16  MAKSPALFHASHFFIRSTLMPSSKSLSLFLPLSNLNHLHRFAFRNSLSPLSLRTFSTT-T 75

Query: 61  PFPLQYEMIINRP-YPLPPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISE 120
            FPLQYE+IINRP YP PPHQN RTPAR+SSDNSPEL+SSED TSELGF+SW+DRKLISE
Sbjct: 76  LFPLQYELIINRPSYPSPPHQNPRTPARVSSDNSPELNSSEDPTSELGFDSWVDRKLISE 135

Query: 121 SEAVSGRG-----------------------------------ICLKPEVVEFNTLHKRE 180
              VSG+                                    + LKPEVVEFNTLHKRE
Sbjct: 136 GGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQAEGFVELKPEVVEFNTLHKRE 195

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLEK YFPD+GLDKAF GPGESNVEVNEQTK R+GV+
Sbjct: 196 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVR 255

Query: 241 KAVGVKPETKVEVPNGMNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKD 300
           KA  VKPE  VEV NGM+DK +VFFDEGKPEK+NKGSV DV+EKKVEEFFKCLKK PAKD
Sbjct: 256 KAGRVKPEMNVEVANGMDDKRMVFFDEGKPEKENKGSVVDVSEKKVEEFFKCLKKGPAKD 315

Query: 301 SEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGT 360
           S IGQ +PYLLTRH ELPAKWDSP GTVVLLNKPKGWTSFTVCGKL RLVKVKKVGHAGT
Sbjct: 316 SNIGQGEPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 375

Query: 361 LDPMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKA KLVDRYQGM  SYSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 376 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 435

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDI+KAAASFCGEIWQVPPMFSAIKVGGE+MYEKARRGESIELSPR+ISIFKFDIERSLD
Sbjct: 436 DDIQKAAASFCGEIWQVPPMFSAIKVGGERMYEKARRGESIELSPRQISIFKFDIERSLD 495

BLAST of Clc03G11400 vs. TAIR 10
Match: AT5G14460.1 (Pseudouridine synthase family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 5.8e-143
Identity = 300/516 (58.14%), Postives = 347/516 (67.25%), Query Frame = 0

Query: 19  LMASSKSLTLFLPL--SNLNHLHGFAFRNSLSSLSLCRFSTTSTPFPLQYEMIINRPYPL 78
           LM+SS  +++  PL  S++        +  L+SL L   STTST +PLQY+MIINRP   
Sbjct: 10  LMSSSARMSMTSPLFFSSVKPWRNI-HKPYLASLFL---STTSTRYPLQYDMIINRPTQS 69

Query: 79  PPHQNGRTPARISSDNSPELHSSEDSTSELGFESWMDRKLISESE-AVSGRG-------- 138
              QN R P +     +P+       ++E  F+SW+D KL  E E    G G        
Sbjct: 70  SLSQNRRRPPKAIESGAPD-------SAEPEFDSWVDNKLAMEREQGRPGSGDPEMDKAK 129

Query: 139 -----------------------------ICLKPEVVEFNTLHKREEELFFYDAFAYPWE 198
                                        + LKPEVVEF+ LH+REEEL+FYD FAYPWE
Sbjct: 130 RKYYSKRRKRLYGSDSEDENSSRKSDEGFVELKPEVVEFDRLHQREEELYFYDTFAYPWE 189

Query: 199 KDKHYKMVYQLEKIYFPDEGLDKAFSGPGESNVEVNEQTKARKGVKKAVGVKPETKVEVP 258
           KDKHYKMVYQLEK Y+PD+ LDKAF  PGE   + ++  K R G KK V      + EV 
Sbjct: 190 KDKHYKMVYQLEKKYYPDQCLDKAFLQPGEVLKKSDDSGKVR-GKKKVVAALGGKRSEVK 249

Query: 259 N-GM-----NDKGLVFFDEGKPEKDNKGSVKD---VAEKKVEEFFKCLKKVPAKD---SE 318
             GM     +D  LVFFDE K +++ K S  D   V EKKVE+FFK L K P +    S 
Sbjct: 250 RIGMENCDEDDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQFFKGLTKSPNEKGMASG 309

Query: 319 IGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLD 378
            G  +P+L+TR+ ELP +WD P+GTV+L+NKPKGWTSFTVCGKL RLVKVKKVGHAGTLD
Sbjct: 310 GGDGEPFLVTRNGELPPRWDGPNGTVLLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLD 369

Query: 379 PMATGLLIVCVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDD 438
           PMATGLLIVCVGKA K+VDRYQGM   YSGVFRLGEATST DADSPVIQRE WEHIKDDD
Sbjct: 370 PMATGLLIVCVGKATKVVDRYQGMIKGYSGVFRLGEATSTLDADSPVIQRESWEHIKDDD 429

Query: 439 IKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLDDR 481
           IKKA  SF GEIWQVPPMFSAIKVGGEKMYEKARRGE++ELSPRRISIF+FDIERSLDDR
Sbjct: 430 IKKALTSFLGEIWQVPPMFSAIKVGGEKMYEKARRGETVELSPRRISIFQFDIERSLDDR 489

BLAST of Clc03G11400 vs. TAIR 10
Match: AT3G57150.1 (homologue of NAP57 )

HSP 1 Score: 84.3 bits (207), Expect = 3.2e-16
Identity = 59/178 (33.15%), Postives = 89/178 (50.00%), Query Frame = 0

Query: 292 VVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKAMKLVDRYQ--G 351
           V+ L+KP   +S  V   + R+++V+K GH+GTLDP  TG LIVC+ +A +LV   Q  G
Sbjct: 82  VINLDKPANPSSHEVVAWIKRILRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAG 141

Query: 352 MSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKVG 411
             Y  V RL  A        P + +          + +A  S  G ++Q PP+ SA+K  
Sbjct: 142 KEYVCVARLHSAV-------PDVAK----------VARALESLTGAVFQRPPLISAVK-- 201

Query: 412 GEKMYEKARRGESIELSPRRISIFKFDIERSLDDRQSLIFRVTCSKGTYIRSPCADLG 468
                 +  R  +I  S     + ++D +R L     ++F V+C  GTYIR+ C  LG
Sbjct: 202 ------RQLRIRTIYES----KLLEYDADRHL-----VVFWVSCEAGTYIRTMCVHLG 225

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0037597.13.5e-22780.69TruB_N domain-containing protein [Cucumis melo var. makuwa] >TYK09336.1 TruB_N d... [more]
XP_008458830.13.5e-22780.69PREDICTED: uncharacterized protein LOC103498118 [Cucumis melo][more]
XP_038891059.17.9e-22780.50uncharacterized protein LOC120080471 [Benincasa hispida][more]
XP_022938741.12.1e-22480.31uncharacterized protein LOC111444875 [Cucurbita moschata] >KAG7016215.1 truB [Cu... [more]
KAG6578676.14.8e-22480.12hypothetical protein SDJN03_23124, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q9RB362.9e-3840.64tRNA pseudouridine synthase B OS=Flavobacterium johnsoniae (strain ATCC 17061 / ... [more]
Q5LJ294.6e-3643.92tRNA pseudouridine synthase B OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2... [more]
Q650L84.6e-3643.92tRNA pseudouridine synthase B OS=Bacteroides fragilis (strain YCH46) OX=295405 G... [more]
A6LEL74.6e-3643.23tRNA pseudouridine synthase B OS=Parabacteroides distasonis (strain ATCC 8503 / ... [more]
Q47RU84.6e-3643.24tRNA pseudouridine synthase B OS=Thermobifida fusca (strain YX) OX=269800 GN=tru... [more]
Match NameE-valueIdentityDescription
A0A5A7T7S01.7e-22780.69TruB_N domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C8B01.7e-22780.69uncharacterized protein LOC103498118 OS=Cucumis melo OX=3656 GN=LOC103498118 PE=... [more]
A0A6J1FDZ71.0e-22480.31uncharacterized protein LOC111444875 OS=Cucurbita moschata OX=3662 GN=LOC1114448... [more]
A0A6J1JSH82.3e-22480.12uncharacterized protein LOC111489372 OS=Cucurbita maxima OX=3661 GN=LOC111489372... [more]
A0A0A0KP032.3e-22479.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G522940 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G14460.15.8e-14358.14Pseudouridine synthase family protein [more]
AT3G57150.13.2e-1633.15homologue of NAP57 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014780tRNA pseudouridine synthase II, TruBTIGRFAMTIGR00431TIGR00431coord: 291..476
e-value: 6.6E-52
score: 174.2
IPR014780tRNA pseudouridine synthase II, TruBPANTHERPTHR13767TRNA-PSEUDOURIDINE SYNTHASEcoord: 264..475
IPR014780tRNA pseudouridine synthase II, TruBHAMAPMF_01080TruB_bactcoord: 289..532
score: 14.407361
IPR014780tRNA pseudouridine synthase II, TruBCDDcd02573PseudoU_synth_EcTruBcoord: 292..469
e-value: 6.38276E-82
score: 252.365
IPR002501Pseudouridine synthase II, N-terminalPFAMPF01509TruB_Ncoord: 312..459
e-value: 4.4E-48
score: 163.4
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 283..494
e-value: 2.0E-68
score: 232.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..105
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 291..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc03G11400.1Clc03G11400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990481 mRNA pseudouridine synthesis
biological_process GO:0006400 tRNA modification
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
biological_process GO:0006396 RNA processing
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding