Clc03G08850 (gene) Watermelon (cordophanus) v2

Overview
NameClc03G08850
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionFAD-binding Berberine family protein
LocationClcChr03: 9209129 .. 9211744 (-)
RNA-Seq ExpressionClc03G08850
SyntenyClc03G08850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTGGTTGAGGAGAATTTATCTTTTTCTTCTCTTTTGTGTTACTTTGATTTGGTTATGTTCTTGTAAATCTCTTCAAGAGAACTTCTTGGAATGCTTCAATTCCACATCTTATTCAAAGCATTCAATTCCCATTTCGGAAGTGGTTTACACAAACGAAAGTGCTTCTTTCTCCTCTCTGCTGCAGCTTTCCATTAGAAATCTCAGATTTCTTACCACTACTTCGCCTAAACCACTGTTTTTGGTGACCCCATTTCATGAGTCTCATGTCCAAGCAGCCATAGTTTGTGCTAGAAAGAAGGGTTTGCAAGTTCGAGTACGTAGTGGAGGACATGACTACGAAGGTTAGAACCTAAAAGCTTAAGTTTAATGTCACCGCTGTGTTTTAGTACATCAATGGTCGGAAAAATTACATTTTTGGTTCTTAGGTTTTATATTTAGTTTCTATTTTGTCTATAAGTTTTAAAATGTTACACTTTTAATTCTTAAATTTTGAATTTGGTTTCACTTAGTCCTTAGACTTCAAAATATTATAATTTTACCTTTCAAATTTCAGCTTTGTGTCAATTTGGCCCCTAGGTTTCAAGATTTATACTTTTAAACTCGATTTTTTACTTAATATTCACTTCCCGATTTTAGTATGAAGAATCAAAATAATTATAATTAATTAAGTTTCATTATCTTACATCACTATATAAATTATAAATTTAAAATTTTATTTCACAACTATTTTAAATTAATTAATAGAGACTAAAAGTATTTTGTGAAAACTAGAGGTGTTAAATGAATGAGAGATGATGATGGTGTTAAGAAAATGTCCACCTAGTGGAGATGTTCCGGTATACCTCTGACTCAATGTATTTATTTATTATTATTTTTTTAAAAAAAAGTACTAATTGAAAATTTTGTATTAGTTGGCTAAATTTTAAAAGGTGGCAAAAAGTGATTTTTAACATATTAAATTCACTTTTAAAAAAATGTTATGGTGAAAAGTGAGTTGAAAAATTTAAAAATAAATTAGAAGACACTTAAAATGCTTTTGGAAGAAGTTTTTTGTTTCTTTTTTCAATATATATATATATCATCTTCACCAAAAGTGCTCATATATTTAATCACACATTTCTCCAAAGGCGCAGCGCACGGGATAGTTTATAAGCATATCCATAAATTTAAATTCTTAACACTTTGAAAATTACCGCAAAGTTTAACACGCGAATACCAATATTAATTAAACGGACAAACTCTATTCGTAGTATTACATGAAATCATCACTGAACCTAAAAGTTACGATGTATTTGAACCAGGTCTCTCTTATGTATCATCACAAGCTCCGTTCATAGTAATTGATCTCATCAACCTTAGCTCCATAATTATCGACATCGAAACCGAAACAGCATTGGTGGGGACCGGTGCCACACTCGGCGAGTTGTACTACAGAATTGCAAAGAAAAGCCCCACCCATGGCTTTCCAGCAGGAAGCTGCCCCACAGTGGGAGTGGGAGGGCATATAAGTGGAGGAGGCTTTGGAACCCTTTTCAGAAAATATGGTCTTGCAGCTGATAATGTAATTGATGCTAAAATTGTTGACTTTAATGGAAGAATTATGGACAGAAACTCCATGGGAGAAGACCTTTTTTGGGCCATTAGAGGAGGTGGAGGAGCAAGCTTTGGAGTGATTTTATCATGGAAACTCAAACTAGTTTCTGTTCCTTCAATTGTCACTGTTTTCAATGTGCAAAGAACTCTAGAACAAGGAGCTACTCAACTTTTCCAAAAATGGCAAACCATTGCACATAAACTTCATCAAGATATTTTCCTTCATGTCACTACAAAAGTTACCAATTTTCCTTCCAAAAAAACAATTAGACTTTCATTTACATCATTGTTTCTTGGACCAATTGAAAGGCTTATCCCATTAATGAAGACTCATTTTCCAGAGTTGGGATTGAAGAGCATTGACTGCACTGAGATGAGTTGGATTCAATCAGTTCTCTTCTTTGCTGACCTACCAACAGAAACCCCACTTGAAGTTTTGATGGAAAGATCACTTCCACTTTCTGAATCATTTTTCACAGCAAAATCTGATTATGTTACATCACCAATTTCGCAAAATGGGTTGGAAGGGCTTTGGACTAAGCTGCTAGAGGAAGAAAAATCTGAGCTGATTTTGACACCATATGGGGGAAAAATGAGCCAAATTTCTAAATCACAAACCCCTTTTCCACATAGAAAAGGGAGCATTTTTGGGATTCAATATTTGGCAACTTGGGATAATGCTAATGAAACTGAGAAGCATTTGAGTTGGATAAGGGAGGTTTACACTTACATGGAACCCTATGTTTCCAAGTCTCCAAGAGCTGCTTATTTGAATTATAGGGATCTTGATTTGGGAAGAAACTATGGTAGAAATACAAGCTATGAACAGGCAAAAGTGTGGGGTTTGAAGTATTTCAGTGATAATTTTGAGAGATTGGTGAAGGTGAAGACCAAAGTTGATCCTTCAAACTTCTTTTGGAATGAACAGAGCATTCCTCTTATCTATCATTATGAAGATGACACAAGAATTGCAAAGGTTTACTCAGGCTTGGACTTTGAAATTGTCCAAGAAAGATGA

mRNA sequence

ATGATTTGGTTGAGGAGAATTTATCTTTTTCTTCTCTTTTGTGTTACTTTGATTTGGTTATGTTCTTGTAAATCTCTTCAAGAGAACTTCTTGGAATGCTTCAATTCCACATCTTATTCAAAGCATTCAATTCCCATTTCGGAAGTGGTTTACACAAACGAAAGTGCTTCTTTCTCCTCTCTGCTGCAGCTTTCCATTAGAAATCTCAGATTTCTTACCACTACTTCGCCTAAACCACTGTTTTTGGTGACCCCATTTCATGAGTCTCATGTCCAAGCAGCCATAGTTTGTGCTAGAAAGAAGGGTTTGCAAGTTCGAGTACGTAGTGGAGGACATGACTACGAAGTATTACATGAAATCATCACTGAACCTAAAAGTTACGATGTATTTGAACCAGGTCTCTCTTATGTATCATCACAAGCTCCGTTCATAGTAATTGATCTCATCAACCTTAGCTCCATAATTATCGACATCGAAACCGAAACAGCATTGGTGGGGACCGGTGCCACACTCGGCGAGTTGTACTACAGAATTGCAAAGAAAAGCCCCACCCATGGCTTTCCAGCAGGAAGCTGCCCCACAGTGGGAGTGGGAGGGCATATAAGTGGAGGAGGCTTTGGAACCCTTTTCAGAAAATATGGTCTTGCAGCTGATAATGTAATTGATGCTAAAATTGTTGACTTTAATGGAAGAATTATGGACAGAAACTCCATGGGAGAAGACCTTTTTTGGGCCATTAGAGGAGGTGGAGGAGCAAGCTTTGGAGTGATTTTATCATGGAAACTCAAACTAGTTTCTGTTCCTTCAATTGTCACTGTTTTCAATGTGCAAAGAACTCTAGAACAAGGAGCTACTCAACTTTTCCAAAAATGGCAAACCATTGCACATAAACTTCATCAAGATATTTTCCTTCATGTCACTACAAAAGTTACCAATTTTCCTTCCAAAAAAACAATTAGACTTTCATTTACATCATTGTTTCTTGGACCAATTGAAAGGCTTATCCCATTAATGAAGACTCATTTTCCAGAGTTGGGATTGAAGAGCATTGACTGCACTGAGATGAGTTGGATTCAATCAGTTCTCTTCTTTGCTGACCTACCAACAGAAACCCCACTTGAAGTTTTGATGGAAAGATCACTTCCACTTTCTGAATCATTTTTCACAGCAAAATCTGATTATGTTACATCACCAATTTCGCAAAATGGGTTGGAAGGGCTTTGGACTAAGCTGCTAGAGGAAGAAAAATCTGAGCTGATTTTGACACCATATGGGGGAAAAATGAGCCAAATTTCTAAATCACAAACCCCTTTTCCACATAGAAAAGGGAGCATTTTTGGGATTCAATATTTGGCAACTTGGGATAATGCTAATGAAACTGAGAAGCATTTGAGTTGGATAAGGGAGGTTTACACTTACATGGAACCCTATGTTTCCAAGTCTCCAAGAGCTGCTTATTTGAATTATAGGGATCTTGATTTGGGAAGAAACTATGGTAGAAATACAAGCTATGAACAGGCAAAAGTGTGGGGTTTGAAGTATTTCAGTGATAATTTTGAGAGATTGGTGAAGGTGAAGACCAAAGTTGATCCTTCAAACTTCTTTTGGAATGAACAGAGCATTCCTCTTATCTATCATTATGAAGATGACACAAGAATTGCAAAGGTTTACTCAGGCTTGGACTTTGAAATTGTCCAAGAAAGATGA

Coding sequence (CDS)

ATGATTTGGTTGAGGAGAATTTATCTTTTTCTTCTCTTTTGTGTTACTTTGATTTGGTTATGTTCTTGTAAATCTCTTCAAGAGAACTTCTTGGAATGCTTCAATTCCACATCTTATTCAAAGCATTCAATTCCCATTTCGGAAGTGGTTTACACAAACGAAAGTGCTTCTTTCTCCTCTCTGCTGCAGCTTTCCATTAGAAATCTCAGATTTCTTACCACTACTTCGCCTAAACCACTGTTTTTGGTGACCCCATTTCATGAGTCTCATGTCCAAGCAGCCATAGTTTGTGCTAGAAAGAAGGGTTTGCAAGTTCGAGTACGTAGTGGAGGACATGACTACGAAGTATTACATGAAATCATCACTGAACCTAAAAGTTACGATGTATTTGAACCAGGTCTCTCTTATGTATCATCACAAGCTCCGTTCATAGTAATTGATCTCATCAACCTTAGCTCCATAATTATCGACATCGAAACCGAAACAGCATTGGTGGGGACCGGTGCCACACTCGGCGAGTTGTACTACAGAATTGCAAAGAAAAGCCCCACCCATGGCTTTCCAGCAGGAAGCTGCCCCACAGTGGGAGTGGGAGGGCATATAAGTGGAGGAGGCTTTGGAACCCTTTTCAGAAAATATGGTCTTGCAGCTGATAATGTAATTGATGCTAAAATTGTTGACTTTAATGGAAGAATTATGGACAGAAACTCCATGGGAGAAGACCTTTTTTGGGCCATTAGAGGAGGTGGAGGAGCAAGCTTTGGAGTGATTTTATCATGGAAACTCAAACTAGTTTCTGTTCCTTCAATTGTCACTGTTTTCAATGTGCAAAGAACTCTAGAACAAGGAGCTACTCAACTTTTCCAAAAATGGCAAACCATTGCACATAAACTTCATCAAGATATTTTCCTTCATGTCACTACAAAAGTTACCAATTTTCCTTCCAAAAAAACAATTAGACTTTCATTTACATCATTGTTTCTTGGACCAATTGAAAGGCTTATCCCATTAATGAAGACTCATTTTCCAGAGTTGGGATTGAAGAGCATTGACTGCACTGAGATGAGTTGGATTCAATCAGTTCTCTTCTTTGCTGACCTACCAACAGAAACCCCACTTGAAGTTTTGATGGAAAGATCACTTCCACTTTCTGAATCATTTTTCACAGCAAAATCTGATTATGTTACATCACCAATTTCGCAAAATGGGTTGGAAGGGCTTTGGACTAAGCTGCTAGAGGAAGAAAAATCTGAGCTGATTTTGACACCATATGGGGGAAAAATGAGCCAAATTTCTAAATCACAAACCCCTTTTCCACATAGAAAAGGGAGCATTTTTGGGATTCAATATTTGGCAACTTGGGATAATGCTAATGAAACTGAGAAGCATTTGAGTTGGATAAGGGAGGTTTACACTTACATGGAACCCTATGTTTCCAAGTCTCCAAGAGCTGCTTATTTGAATTATAGGGATCTTGATTTGGGAAGAAACTATGGTAGAAATACAAGCTATGAACAGGCAAAAGTGTGGGGTTTGAAGTATTTCAGTGATAATTTTGAGAGATTGGTGAAGGTGAAGACCAAAGTTGATCCTTCAAACTTCTTTTGGAATGAACAGAGCATTCCTCTTATCTATCATTATGAAGATGACACAAGAATTGCAAAGGTTTACTCAGGCTTGGACTTTGAAATTGTCCAAGAAAGATGA

Protein sequence

MIWLRRIYLFLLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIFLHVTTKVTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLIYHYEDDTRIAKVYSGLDFEIVQER
Homology
BLAST of Clc03G08850 vs. NCBI nr
Match: XP_038895849.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 994.2 bits (2569), Expect = 4.6e-286
Identity = 507/571 (88.79%), Postives = 527/571 (92.29%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCK-SLQENFLECFNSTSYSKHSIPISEVVYTNESASFS 60
           M+ LRR++LFLLF V+LIWLCS   SLQE FLECFNSTSYSKHSIPISEVV+TNESASFS
Sbjct: 1   MLCLRRVHLFLLFFVSLIWLCSSSDSLQERFLECFNSTSYSKHSIPISEVVFTNESASFS 60

Query: 61  SLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHE 120
           SLLQLSIRNLRFLTTT PKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYE    
Sbjct: 61  SLLQLSIRNLRFLTTTMPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYE---- 120

Query: 121 IITEPKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIA 180
                        GLSYVSSQAPFIVIDLINL SI IDIETET  V TGATLGELYY+IA
Sbjct: 121 -------------GLSYVSSQAPFIVIDLINLHSITIDIETETTSVETGATLGELYYKIA 180

Query: 181 KKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMG 240
           +KSP HGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMG
Sbjct: 181 EKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMG 240

Query: 241 EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLH 300
           EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGAT LFQKWQTIAHKL 
Sbjct: 241 EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQTIAHKLD 300

Query: 301 QDIFLHVTTKV-TNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWI 360
           QDIFLHVTTKV TNF SKKTIRLSFTSLFLGPIERLIP+MKTHF ELGLK  DCTEMSWI
Sbjct: 301 QDIFLHVTTKVITNFSSKKTIRLSFTSLFLGPIERLIPIMKTHFSELGLKRNDCTEMSWI 360

Query: 361 QSVLFFADLPTETPLEVLMERSL-PLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKS 420
           QSVLFFAD   ETPLEVLMERS  P+S++FFTAKSDYVTSPISQNGLEGLWTKLLEEEKS
Sbjct: 361 QSVLFFADFSIETPLEVLMERSSPPISDAFFTAKSDYVTSPISQNGLEGLWTKLLEEEKS 420

Query: 421 ELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPY 480
           ELILTPYGGKMSQIS+SQTPFPHR+GSIFGIQYLATWDNANETEKHLSWIREVYTYMEPY
Sbjct: 421 ELILTPYGGKMSQISESQTPFPHRRGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPY 480

Query: 481 VSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWN 540
           VSKSPRAAYLNYRDLDLGRNY +NTSYE+AKVWGLKYFSDNFERLV+VKTKVDP NFFWN
Sbjct: 481 VSKSPRAAYLNYRDLDLGRNYRKNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPLNFFWN 540

Query: 541 EQSIPLIYHYEDDTRIAKVYSGLDFEIVQER 569
           EQSIPL+YHYEDDTRI+KV+SGLDFEIVQER
Sbjct: 541 EQSIPLLYHYEDDTRISKVHSGLDFEIVQER 554

BLAST of Clc03G08850 vs. NCBI nr
Match: XP_008445632.1 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0047127.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa] >TYK14944.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa])

HSP 1 Score: 976.9 bits (2524), Expect = 7.7e-281
Identity = 497/571 (87.04%), Postives = 520/571 (91.07%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCK-SLQENFLECFNSTSYSKHSIPISEVVYTNESASFS 60
           MI LRR +LFLLFCV+LIWLCS   SLQ+NFLECFNSTSYSKHSIP+SEVV+TNESASFS
Sbjct: 3   MICLRRTHLFLLFCVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFS 62

Query: 61  SLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHE 120
           SL +LSIRNLRFLTTT PKPLFLVTPFHESHVQAAIVCAR+KGLQVRVRSGGHDYE    
Sbjct: 63  SLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYE---- 122

Query: 121 IITEPKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIA 180
                        GLSYVSSQAPFIVIDLINL SI IDIE ETA VGTGA LGELYYRIA
Sbjct: 123 -------------GLSYVSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIA 182

Query: 181 KKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMG 240
           KKS  HGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMG
Sbjct: 183 KKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMG 242

Query: 241 EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLH 300
           EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGAT LFQKWQ I+HKL 
Sbjct: 243 EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLD 302

Query: 301 QDIFLHVTTK-VTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWI 360
           QDIFLHVTTK VT+FPSKKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSWI
Sbjct: 303 QDIFLHVTTKVVTDFPSKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWI 362

Query: 361 QSVLFFADLPTETPLEVLMERSLP-LSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKS 420
           QSVLFFAD   +TPLEVLMERS P +S++FFTAKSDYV SPISQ GLEGLW KLLEEEKS
Sbjct: 363 QSVLFFADFSIDTPLEVLMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKS 422

Query: 421 ELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPY 480
           ELILTPYGGKMSQIS+SQTPFPHR+G IFGIQYLATWDNANETEKHLSWIREVY YM+PY
Sbjct: 423 ELILTPYGGKMSQISESQTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPY 482

Query: 481 VSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWN 540
           VSKSPRAAYLNYRDLDLGRNYGRNTSYE+AKVWGLKYFSDNFERLV+VKTKVDPSNFFWN
Sbjct: 483 VSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWN 542

Query: 541 EQSIPLIYHYEDDTRIAKVYSGLDFEIVQER 569
           EQSIPL+YHYEDDTRI KV+SGLDFEIVQER
Sbjct: 543 EQSIPLLYHYEDDTRITKVHSGLDFEIVQER 556

BLAST of Clc03G08850 vs. NCBI nr
Match: KAE8653305.1 (hypothetical protein Csa_023319 [Cucumis sativus])

HSP 1 Score: 956.8 bits (2472), Expect = 8.2e-275
Identity = 484/572 (84.62%), Postives = 517/572 (90.38%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLC--SCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASF 60
           MI  RRI+LFLL CV+LIWLC  S  SLQ+NFLECFNSTSYSKHSIPISEVV+TNESASF
Sbjct: 3   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 62

Query: 61  SSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLH 120
           SSLL+LSIRNLRFLTTT PKPL LVTPFHESHVQAAI+CAR+KGLQVRVRSGGHDYE   
Sbjct: 63  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYE--- 122

Query: 121 EIITEPKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRI 180
                         GLSY+SSQAPFIVIDLINL SI IDI+TETA V TGA+LGELYYRI
Sbjct: 123 --------------GLSYISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRI 182

Query: 181 AKKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSM 240
           AKKS  HGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SM
Sbjct: 183 AKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSM 242

Query: 241 GEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKL 300
           GEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLE+GAT LFQKWQ I+HKL
Sbjct: 243 GEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKL 302

Query: 301 HQDIFLHVTTK-VTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSW 360
            QDIFLHVTTK VTNFPSKKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSW
Sbjct: 303 DQDIFLHVTTKVVTNFPSKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSW 362

Query: 361 IQSVLFFADLPTETPLEVLMERSLP-LSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEK 420
           IQSVLFFAD   + PLEVLM+RS P +S++FFTAKSDYVTSPIS+NGLEGLW+KLLEE+K
Sbjct: 363 IQSVLFFADFSIDAPLEVLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDK 422

Query: 421 SELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEP 480
           SELI TPYGGKMSQIS+SQ PFPHR+G IFGIQYLATWDNANE EKHLSWIREVY YME 
Sbjct: 423 SELIFTPYGGKMSQISESQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMES 482

Query: 481 YVSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFW 540
           YVSKSPRAAYLNYRDLDLG NYGRNTSYE+AKVWGLKYFSDNF+RLV+VKTKVDPSNFFW
Sbjct: 483 YVSKSPRAAYLNYRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFW 542

Query: 541 NEQSIPLIYHYEDDTRIAKVYSGLDFEIVQER 569
           NEQSIPL+YHYEDDT++ KV+SGLDFEI+QER
Sbjct: 543 NEQSIPLLYHYEDDTKVTKVHSGLDFEIIQER 557

BLAST of Clc03G08850 vs. NCBI nr
Match: XP_004135724.1 (berberine bridge enzyme-like 13 [Cucumis sativus])

HSP 1 Score: 956.8 bits (2472), Expect = 8.2e-275
Identity = 484/572 (84.62%), Postives = 517/572 (90.38%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLC--SCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASF 60
           MI  RRI+LFLL CV+LIWLC  S  SLQ+NFLECFNSTSYSKHSIPISEVV+TNESASF
Sbjct: 1   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 60

Query: 61  SSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLH 120
           SSLL+LSIRNLRFLTTT PKPL LVTPFHESHVQAAI+CAR+KGLQVRVRSGGHDYE   
Sbjct: 61  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYE--- 120

Query: 121 EIITEPKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRI 180
                         GLSY+SSQAPFIVIDLINL SI IDI+TETA V TGA+LGELYYRI
Sbjct: 121 --------------GLSYISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRI 180

Query: 181 AKKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSM 240
           AKKS  HGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SM
Sbjct: 181 AKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSM 240

Query: 241 GEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKL 300
           GEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLE+GAT LFQKWQ I+HKL
Sbjct: 241 GEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKL 300

Query: 301 HQDIFLHVTTK-VTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSW 360
            QDIFLHVTTK VTNFPSKKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSW
Sbjct: 301 DQDIFLHVTTKVVTNFPSKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSW 360

Query: 361 IQSVLFFADLPTETPLEVLMERSLP-LSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEK 420
           IQSVLFFAD   + PLEVLM+RS P +S++FFTAKSDYVTSPIS+NGLEGLW+KLLEE+K
Sbjct: 361 IQSVLFFADFSIDAPLEVLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDK 420

Query: 421 SELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEP 480
           SELI TPYGGKMSQIS+SQ PFPHR+G IFGIQYLATWDNANE EKHLSWIREVY YME 
Sbjct: 421 SELIFTPYGGKMSQISESQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMES 480

Query: 481 YVSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFW 540
           YVSKSPRAAYLNYRDLDLG NYGRNTSYE+AKVWGLKYFSDNF+RLV+VKTKVDPSNFFW
Sbjct: 481 YVSKSPRAAYLNYRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFW 540

Query: 541 NEQSIPLIYHYEDDTRIAKVYSGLDFEIVQER 569
           NEQSIPL+YHYEDDT++ KV+SGLDFEI+QER
Sbjct: 541 NEQSIPLLYHYEDDTKVTKVHSGLDFEIIQER 555

BLAST of Clc03G08850 vs. NCBI nr
Match: XP_022140178.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 882.9 bits (2280), Expect = 1.5e-252
Identity = 450/575 (78.26%), Postives = 496/575 (86.26%), Query Frame = 0

Query: 6   RIYLFLLF--CVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQ 65
           RI+LFL+F  CV+     S   LQE+F+ECFNST+YSKHS+PISEVV+TNESASFSSLL+
Sbjct: 8   RIHLFLIFFHCVS-----SSDCLQESFMECFNSTTYSKHSVPISEVVFTNESASFSSLLR 67

Query: 66  LSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITE 125
           LSIRNLRFLT +SPKP FLVTPF  SHVQAA+VCA+KK LQVRVRSGGHDYE        
Sbjct: 68  LSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYE-------- 127

Query: 126 PKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSP 185
                    GLSYVSSQ PFIVIDLINLSSI IDIETETA V TGATLGE+YYRIA+KSP
Sbjct: 128 ---------GLSYVSSQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSP 187

Query: 186 THGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLF 245
            HGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKIVDFNGRI DRNSMGEDLF
Sbjct: 188 IHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLF 247

Query: 246 WAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIF 305
           WAIRGGGGASFGVILSWKLKLVSVP  VTVFNV RTL+QGAT LFQKWQ IAHK+ QD+F
Sbjct: 248 WAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLF 307

Query: 306 LHVTTKVTNFPSK---------KTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTE 365
           LHVTTKV +  +K         KTI+LSFTSLFLGPIERLIPLM THF ELGLK  DCTE
Sbjct: 308 LHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTE 367

Query: 366 MSWIQSVLFFADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEE 425
           MSWIQSVLFFADL TETP E+LMERS P+S++FFTAKSDYVTSPISQ GLE LW+KLLEE
Sbjct: 368 MSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEE 427

Query: 426 EKSELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYM 485
           EKSELILTPYGGKM QIS+S+TPFPHR+G+I+GIQYLA W+NANETEKHLSW+REVY+YM
Sbjct: 428 EKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYM 487

Query: 486 EPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNF 545
           +P+VSK PR AYLNYRDLDLGRNY RNTSY++AKVWGLKYF  NFERLV+VKTKVDP NF
Sbjct: 488 QPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNF 547

Query: 546 FWNEQSIPLIYHYEDD-TRIAKVYSGLDFEIVQER 569
           FWNEQSIPL +H+E++ TRI +V S LD +I+QER
Sbjct: 548 FWNEQSIPLCHHHEENGTRILQVNSYLDSKILQER 560

BLAST of Clc03G08850 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 8.0e-148
Identity = 267/539 (49.54%), Postives = 362/539 (67.16%), Query Frame = 0

Query: 12  LFCVTLIWL---CSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRN 71
           LF VTL+ +    S  +LQ++F++C    S     I  S       +  F   L+ + +N
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 71

Query: 72  LRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKSYD 131
           LR+LT ++PKP+F+  P +E+HVQAA+VCA+K  L +R+RSGGHDYE             
Sbjct: 72  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYE------------- 131

Query: 132 VFEPGLSYVS-SQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTHGF 191
               GLS+V+  + PF+++DL  L  + +D+++ +A    GAT+GE+YYRI +KS THGF
Sbjct: 132 ----GLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGF 191

Query: 192 PAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIR 251
           PAG C ++G+GGH+ GG +G++ RK+GL ADNV+DA+IVD NG+I+DR +MGED+FWAIR
Sbjct: 192 PAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIR 251

Query: 252 GGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIFLHV- 311
           GGGG SFGVIL+WK+KLV VP+ VTVF V +TLEQ  T++  KW+ IA KL  D+F+ V 
Sbjct: 252 GGGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVI 311

Query: 312 --TTKVTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFF 371
                 T  P  +TI +S+ + FLG   RL+ +M+  FPELGL   DCTEMSWI+SV++ 
Sbjct: 312 ISPASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI 371

Query: 372 ADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPY 431
           A  P     E L+     L ++ F AKSD+V  PI   GLEGLW + LEE+    I  PY
Sbjct: 372 AGFPNSAAPEALL-AGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPY 431

Query: 432 GGKMSQISKSQTPFPHRKGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPR 491
           GG MS+IS+S+ PFPHR G++F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR
Sbjct: 432 GGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPR 491

Query: 492 AAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 543
            AY+NYRDLDLG N G       A+ WG KY+  NFERLVK+K + DP NFF +EQS+P
Sbjct: 492 QAYVNYRDLDLGTNEGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Clc03G08850 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.4e-147
Identity = 265/542 (48.89%), Postives = 368/542 (67.90%), Query Frame = 0

Query: 9   LFLLFCVTLIWLCSCKSL-QENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIR 68
           + +LF V  IW  S  S   E F +C  S S  KH  PIS  ++ + + S+SS+LQ +IR
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANIR 65

Query: 69  NLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKSY 128
           NLRF TT++PKP  ++   HESHVQAAI C ++  LQ+++RSGGHDY+            
Sbjct: 66  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYD------------ 125

Query: 129 DVFEPGLSYVS-SQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTHG 188
                GLSYV+ S  PF V+D+ NL S+ +D+ ++TA V TGA LGE+YY I +KS T  
Sbjct: 126 -----GLSYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLA 185

Query: 189 FPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAI 248
           +PAG CPTVGVGGHISGGG+G + RKYGL  DN IDA++VD NG+I+DR  MGEDL+WAI
Sbjct: 186 YPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAI 245

Query: 249 RGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIFLHV 308
            GGGG S+GV+L++K+ LV VP  VTVF + RTLEQ AT +  +WQ +A KL  ++F+  
Sbjct: 246 NGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRT 305

Query: 309 TTKVTN--FPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFF 368
              V N    S+KT+R +F ++FLG    L+ ++   FPELGL   DCTE SWIQSVLF+
Sbjct: 306 VIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFW 365

Query: 369 ADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPY 428
            ++   +   +L++R+ P+  ++   KSDYV  PIS+ GLE +W K++E E   +   PY
Sbjct: 366 TNIQVGSSETLLLQRNQPV--NYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPY 425

Query: 429 GGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRA 488
           GG+M +IS + TPFP+R G+++ IQY A W +   T++++   R++Y +M P+VSK+PR 
Sbjct: 426 GGEMGRISSTVTPFPYRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQ 485

Query: 489 AYLNYRDLDLGRN--YGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 545
           ++ NYRD+DLG N   G+ +SY + K +G KYF+ NFERLVK+KT+VD  NFF NEQSIP
Sbjct: 486 SFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 526

BLAST of Clc03G08850 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 5.2e-147
Identity = 269/541 (49.72%), Postives = 367/541 (67.84%), Query Frame = 0

Query: 13  FCVTLIWL------CSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSI 72
           F VTL+ L       S  ++Q++F+ C    S +   +  S   +   ++SF   L+ S 
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 71

Query: 73  RNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKS 132
           +NLR+L  ++PKP F+  P +E+HVQAA++CA+K  L +R+RSGGHDYE           
Sbjct: 72  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYE----------- 131

Query: 133 YDVFEPGLSYVSS-QAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTH 192
                 GLSYVS  +  F+++DL  L  I +DIE+ +A V  GA++GE+YYRI +KS  H
Sbjct: 132 ------GLSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIH 191

Query: 193 GFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWA 252
           GFPAG C ++G+GGHI GG +G++ RK+GL ADNV+DA+IVD +G+I++R +MGED+FWA
Sbjct: 192 GFPAGLCTSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWA 251

Query: 253 IRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIFLH 312
           IRGGGG SFGVIL+WK+KLV VP IVTVF V RTLEQ  T+L  KWQ +A KL +D+F+ 
Sbjct: 252 IRGGGGGSFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIR 311

Query: 313 VTTKVTNFPSK---KTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVL 372
           V  + T+   K   +TI  S+   FLG   RL+ +M+  FP+LGL   DC E SWI+SV+
Sbjct: 312 VIIQPTSKTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVM 371

Query: 373 FFADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILT 432
           + A  P+  P E L++    L +++F AKSDYV  PI   GLEGLW KLLEE+    I  
Sbjct: 372 YIAGFPSTAPSEALLD-GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWN 431

Query: 433 PYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETE-KHLSWIREVYTYMEPYVSKS 492
           PYGG M++I +++TPFPHR G++F IQ+L  W +   +E KH+ W+RE+Y+YME YVSKS
Sbjct: 432 PYGGMMAKIPETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKS 491

Query: 493 PRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSI 543
           PR+AY+NYRDLDLG N G+ +    A+ WG +YF  NFERLV++K K DP NFF +EQSI
Sbjct: 492 PRSAYVNYRDLDLGMN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSI 530

BLAST of Clc03G08850 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.5e-146
Identity = 262/540 (48.52%), Postives = 364/540 (67.41%), Query Frame = 0

Query: 7   IYLFLLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSI 66
           +  F L+ +T     S  SLQ+ F+ C    ++    +  +    T   + FS +L+ + 
Sbjct: 14  VLYFSLYTITP--TSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTA 73

Query: 67  RNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKS 126
           +NLRFL  + PKP F+ +P HESHVQA+I+C++K  + +RVRSGGHDYE           
Sbjct: 74  QNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYE----------- 133

Query: 127 YDVFEPGLSYVSS-QAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTH 186
                 GLSYVS    PFI++DL  +  + I+I+  +A V +GAT+GELYYRIA+KS  H
Sbjct: 134 ------GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVH 193

Query: 187 GFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWA 246
           GFPAG C ++G+GGHI+GG +G++ RKYGL ADNV+DAKIVD NG+++DR +MGED FWA
Sbjct: 194 GFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWA 253

Query: 247 IRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQG-ATQLFQKWQTIAHKLHQDIFL 306
           IRGG G SFG+IL+WK+KLV VP  VTVF V +TL+Q    ++  KWQ +A KL +++F+
Sbjct: 254 IRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFI 313

Query: 307 HVTTKVTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFF 366
            V   V      KT+  S+ +LFLG    L+ +MK  FPELGL   DC EMSW++S+ + 
Sbjct: 314 RVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYI 373

Query: 367 ADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPY 426
           +  PT TP  VL++   P  +  F AKSD+V +PI ++GL+G++ KLL+E+   +I  PY
Sbjct: 374 SGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPY 433

Query: 427 GGKMSQISKSQTPFPHRKGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPR 486
           GG M++I +SQ PFPHRKG +F +QY+ +W D+     +H++WIR++Y+YM PYVS +PR
Sbjct: 434 GGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPR 493

Query: 487 AAYLNYRDLDLGRN-YGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 543
            AY+NYRDLDLGRN     T  +QA+VWG  YF +NF RL+ +K KVDP NFF +EQSIP
Sbjct: 494 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of Clc03G08850 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 5.7e-146
Identity = 265/540 (49.07%), Postives = 362/540 (67.04%), Query Frame = 0

Query: 11  LLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRNLR 70
           L F    I L S  SLQ++F++C    +  + ++  +       ++ F+ +L+ + +N R
Sbjct: 18  LYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLESTAQNQR 77

Query: 71  FLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKSYDVF 130
           +LT T PKP F+  P HESHVQA+++C++K  +  RVRSGGHDYE               
Sbjct: 78  YLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYE--------------- 137

Query: 131 EPGLSYVSS-QAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTHGFPA 190
             G+SYVS  + PF++IDL  L  I +DI+  +A V  GAT+GELYYRIA+KS  HGFPA
Sbjct: 138 --GVSYVSQIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPA 197

Query: 191 GSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGG 250
           G  P++G+GGHI+GG +G+L RKYGLAADNV+DAKIVD NG+++DR SMGEDLFWAIRGG
Sbjct: 198 GVYPSLGIGGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGG 257

Query: 251 GGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGAT-QLFQKWQTIAHKLHQDIFLHVTT 310
            G SFG+ILSWK+KLV VP  +TVF V +T EQ  + ++  KWQ IA  L  ++FL V  
Sbjct: 258 SGGSFGIILSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFF 317

Query: 311 KVTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLP 370
            V+   + KT+ +++   FLG    L+ +MK  FPELGL   DC EMSWI S+++ +  P
Sbjct: 318 TVSGNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFP 377

Query: 371 TE--TPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGG 430
           T    P+E+L++   P+ + +F  KSD+   PI   GLEG++ KLLEE+ + +I TPYGG
Sbjct: 378 TNPPPPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGG 437

Query: 431 KMSQISKSQTPFPHRKGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAA 490
           KM +I +S+ PFPHR G+ F IQY  +W D+     +   WIRE+Y YM PYVS +PR A
Sbjct: 438 KMDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQA 497

Query: 491 YLNYRDLDLGRNYGRNTS-YEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLI 545
           Y+NYRDLDLG+N   + S + +AK+WG  YF DNF RLV++K+KVDP NFF +EQSIP +
Sbjct: 498 YVNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of Clc03G08850 vs. ExPASy TrEMBL
Match: A0A5A7TYX1 (Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold437G00060 PE=3 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 3.7e-281
Identity = 497/571 (87.04%), Postives = 520/571 (91.07%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCK-SLQENFLECFNSTSYSKHSIPISEVVYTNESASFS 60
           MI LRR +LFLLFCV+LIWLCS   SLQ+NFLECFNSTSYSKHSIP+SEVV+TNESASFS
Sbjct: 3   MICLRRTHLFLLFCVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFS 62

Query: 61  SLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHE 120
           SL +LSIRNLRFLTTT PKPLFLVTPFHESHVQAAIVCAR+KGLQVRVRSGGHDYE    
Sbjct: 63  SLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYE---- 122

Query: 121 IITEPKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIA 180
                        GLSYVSSQAPFIVIDLINL SI IDIE ETA VGTGA LGELYYRIA
Sbjct: 123 -------------GLSYVSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIA 182

Query: 181 KKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMG 240
           KKS  HGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMG
Sbjct: 183 KKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMG 242

Query: 241 EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLH 300
           EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGAT LFQKWQ I+HKL 
Sbjct: 243 EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLD 302

Query: 301 QDIFLHVTTK-VTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWI 360
           QDIFLHVTTK VT+FPSKKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSWI
Sbjct: 303 QDIFLHVTTKVVTDFPSKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWI 362

Query: 361 QSVLFFADLPTETPLEVLMERSLP-LSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKS 420
           QSVLFFAD   +TPLEVLMERS P +S++FFTAKSDYV SPISQ GLEGLW KLLEEEKS
Sbjct: 363 QSVLFFADFSIDTPLEVLMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKS 422

Query: 421 ELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPY 480
           ELILTPYGGKMSQIS+SQTPFPHR+G IFGIQYLATWDNANETEKHLSWIREVY YM+PY
Sbjct: 423 ELILTPYGGKMSQISESQTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPY 482

Query: 481 VSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWN 540
           VSKSPRAAYLNYRDLDLGRNYGRNTSYE+AKVWGLKYFSDNFERLV+VKTKVDPSNFFWN
Sbjct: 483 VSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWN 542

Query: 541 EQSIPLIYHYEDDTRIAKVYSGLDFEIVQER 569
           EQSIPL+YHYEDDTRI KV+SGLDFEIVQER
Sbjct: 543 EQSIPLLYHYEDDTRITKVHSGLDFEIVQER 556

BLAST of Clc03G08850 vs. ExPASy TrEMBL
Match: A0A1S3BE11 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103488588 PE=3 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 3.7e-281
Identity = 497/571 (87.04%), Postives = 520/571 (91.07%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCK-SLQENFLECFNSTSYSKHSIPISEVVYTNESASFS 60
           MI LRR +LFLLFCV+LIWLCS   SLQ+NFLECFNSTSYSKHSIP+SEVV+TNESASFS
Sbjct: 3   MICLRRTHLFLLFCVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFS 62

Query: 61  SLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHE 120
           SL +LSIRNLRFLTTT PKPLFLVTPFHESHVQAAIVCAR+KGLQVRVRSGGHDYE    
Sbjct: 63  SLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYE---- 122

Query: 121 IITEPKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIA 180
                        GLSYVSSQAPFIVIDLINL SI IDIE ETA VGTGA LGELYYRIA
Sbjct: 123 -------------GLSYVSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIA 182

Query: 181 KKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMG 240
           KKS  HGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMG
Sbjct: 183 KKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMG 242

Query: 241 EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLH 300
           EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGAT LFQKWQ I+HKL 
Sbjct: 243 EDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLD 302

Query: 301 QDIFLHVTTK-VTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWI 360
           QDIFLHVTTK VT+FPSKKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSWI
Sbjct: 303 QDIFLHVTTKVVTDFPSKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWI 362

Query: 361 QSVLFFADLPTETPLEVLMERSLP-LSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKS 420
           QSVLFFAD   +TPLEVLMERS P +S++FFTAKSDYV SPISQ GLEGLW KLLEEEKS
Sbjct: 363 QSVLFFADFSIDTPLEVLMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKS 422

Query: 421 ELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPY 480
           ELILTPYGGKMSQIS+SQTPFPHR+G IFGIQYLATWDNANETEKHLSWIREVY YM+PY
Sbjct: 423 ELILTPYGGKMSQISESQTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPY 482

Query: 481 VSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWN 540
           VSKSPRAAYLNYRDLDLGRNYGRNTSYE+AKVWGLKYFSDNFERLV+VKTKVDPSNFFWN
Sbjct: 483 VSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWN 542

Query: 541 EQSIPLIYHYEDDTRIAKVYSGLDFEIVQER 569
           EQSIPL+YHYEDDTRI KV+SGLDFEIVQER
Sbjct: 543 EQSIPLLYHYEDDTRITKVHSGLDFEIVQER 556

BLAST of Clc03G08850 vs. ExPASy TrEMBL
Match: A0A6J1CHF0 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 7.3e-253
Identity = 450/575 (78.26%), Postives = 496/575 (86.26%), Query Frame = 0

Query: 6   RIYLFLLF--CVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQ 65
           RI+LFL+F  CV+     S   LQE+F+ECFNST+YSKHS+PISEVV+TNESASFSSLL+
Sbjct: 8   RIHLFLIFFHCVS-----SSDCLQESFMECFNSTTYSKHSVPISEVVFTNESASFSSLLR 67

Query: 66  LSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITE 125
           LSIRNLRFLT +SPKP FLVTPF  SHVQAA+VCA+KK LQVRVRSGGHDYE        
Sbjct: 68  LSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYE-------- 127

Query: 126 PKSYDVFEPGLSYVSSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSP 185
                    GLSYVSSQ PFIVIDLINLSSI IDIETETA V TGATLGE+YYRIA+KSP
Sbjct: 128 ---------GLSYVSSQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSP 187

Query: 186 THGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLF 245
            HGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKIVDFNGRI DRNSMGEDLF
Sbjct: 188 IHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLF 247

Query: 246 WAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIF 305
           WAIRGGGGASFGVILSWKLKLVSVP  VTVFNV RTL+QGAT LFQKWQ IAHK+ QD+F
Sbjct: 248 WAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLF 307

Query: 306 LHVTTKVTNFPSK---------KTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTE 365
           LHVTTKV +  +K         KTI+LSFTSLFLGPIERLIPLM THF ELGLK  DCTE
Sbjct: 308 LHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTE 367

Query: 366 MSWIQSVLFFADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEE 425
           MSWIQSVLFFADL TETP E+LMERS P+S++FFTAKSDYVTSPISQ GLE LW+KLLEE
Sbjct: 368 MSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEE 427

Query: 426 EKSELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYM 485
           EKSELILTPYGGKM QIS+S+TPFPHR+G+I+GIQYLA W+NANETEKHLSW+REVY+YM
Sbjct: 428 EKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYM 487

Query: 486 EPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNF 545
           +P+VSK PR AYLNYRDLDLGRNY RNTSY++AKVWGLKYF  NFERLV+VKTKVDP NF
Sbjct: 488 QPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNF 547

Query: 546 FWNEQSIPLIYHYEDD-TRIAKVYSGLDFEIVQER 569
           FWNEQSIPL +H+E++ TRI +V S LD +I+QER
Sbjct: 548 FWNEQSIPLCHHHEENGTRILQVNSYLDSKILQER 560

BLAST of Clc03G08850 vs. ExPASy TrEMBL
Match: A0A6J1JUS4 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111489931 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.3e-249
Identity = 445/576 (77.26%), Postives = 490/576 (85.07%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCKSL---QENFLECFNSTSYSKHSIPISEVVYTNESAS 60
           M+  RRI L L F V LIW+CS  S    QENFLECFNST+YSKHS+P+SEVV++NESAS
Sbjct: 1   MVHWRRIQLLLFFWVPLIWICSSSSSDFDQENFLECFNSTTYSKHSLPVSEVVFSNESAS 60

Query: 61  FSSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVL 120
           FSSLL+ SIRNLRFL+T+SPKPLFLVTPFHESHVQAAIVCA++  LQVRV SGGHDYE  
Sbjct: 61  FSSLLKHSIRNLRFLSTSSPKPLFLVTPFHESHVQAAIVCAKENDLQVRVLSGGHDYE-- 120

Query: 121 HEIITEPKSYDVFEPGLSYV-SSQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYY 180
                          GLSY+ SSQAPFIVIDLINL SI I+I+TETALV TGAT+GELYY
Sbjct: 121 ---------------GLSYLSSSQAPFIVIDLINLRSIDINIKTETALVETGATIGELYY 180

Query: 181 RIAKKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRN 240
           RIAKKSP HGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRN
Sbjct: 181 RIAKKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRN 240

Query: 241 SMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAH 300
           +MGEDLFWAIRGGGGASFGVILSWKLKLVS+PSIVT FNVQRTL QGAT LFQKWQ IAH
Sbjct: 241 TMGEDLFWAIRGGGGASFGVILSWKLKLVSLPSIVTTFNVQRTLNQGATHLFQKWQQIAH 300

Query: 301 KLHQDIFLHVTTKVTN----FPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDC 360
           KL QD+FLHVTTKV +      + KT+ LSFTSLFLG I+ LIPLM THFPELGLK   C
Sbjct: 301 KLDQDLFLHVTTKVIDQKMTKTTSKTLSLSFTSLFLGSIDSLIPLMDTHFPELGLKRYQC 360

Query: 361 TEMSWIQSVLFFADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLL 420
           TEM+WIQS+LFFAD  T++PLE+LM+R LP + S F AKSDYVTSPISQ+GLEGLW KLL
Sbjct: 361 TEMNWIQSILFFADFSTKSPLEILMQR-LPSTRSSFIAKSDYVTSPISQSGLEGLWAKLL 420

Query: 421 EEEKSELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYT 480
           E E SELI TPYGGKMSQIS+ +TPFPHRKGSIFG+QYLATWD++NE E+HL WIREVY+
Sbjct: 421 ENENSELIFTPYGGKMSQISELETPFPHRKGSIFGVQYLATWDDSNENERHLRWIREVYS 480

Query: 481 YMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPS 540
           YMEPYVS +PR  YLNYRDLDLGRNYGRNTSYE+AKVWGLKYF  NFERLVKVKTK DP 
Sbjct: 481 YMEPYVSMAPRGTYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFKGNFERLVKVKTKFDPL 540

Query: 541 NFFWNEQSIPLIYHYEDDTRIAKVYSGLDFEIVQER 569
           NFFWNEQSIPL+Y+ ED+ RI +VYS L+ E VQER
Sbjct: 541 NFFWNEQSIPLLYNCEDEARICEVYSDLNSENVQER 558

BLAST of Clc03G08850 vs. ExPASy TrEMBL
Match: A0A6J1GQY4 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111456716 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.7e-244
Identity = 440/579 (75.99%), Postives = 484/579 (83.59%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLC--SCKS----LQENFLECFNSTSYSKHSIPISEVVYTNE 60
           M+  RRI L L F     W+C  SC S    +QENF+ECFNST+YSKHS+P+SEVV++NE
Sbjct: 1   MVHWRRIQLLLFF-----WICSSSCSSSSAFVQENFIECFNSTTYSKHSLPVSEVVFSNE 60

Query: 61  SASFSSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDY 120
           SASFSSLL+ SIRNLRFL+T+SPKPLFLVTPFHESHVQAAIVCA++  LQVRV SGGHDY
Sbjct: 61  SASFSSLLKHSIRNLRFLSTSSPKPLFLVTPFHESHVQAAIVCAKENDLQVRVLSGGHDY 120

Query: 121 EVLHEIITEPKSYDVFEPGLSYV-SSQAPFIVIDLINLSSIIIDIETETALVGTGATLGE 180
           E                 GLSY+ SSQAPFIVIDLINL SI I+IETETA V TGATLGE
Sbjct: 121 E-----------------GLSYLSSSQAPFIVIDLINLRSIDINIETETASVETGATLGE 180

Query: 181 LYYRIAKKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIM 240
           LYYRIAKKSP HGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIM
Sbjct: 181 LYYRIAKKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIM 240

Query: 241 DRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQT 300
           DRN+M EDLFWAIRGGGGASFGVILSWKLKLVS+PSIVT FNVQRTL QG T LF KWQ 
Sbjct: 241 DRNTMEEDLFWAIRGGGGASFGVILSWKLKLVSLPSIVTTFNVQRTLNQGGTHLFLKWQE 300

Query: 301 IAHKLHQDIFLHVTTKVTN----FPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKS 360
           IAHKL+QD+FLHVTTKV +      + KT+ LSFTSLFLG I+ LIPLM THFPELGLK 
Sbjct: 301 IAHKLNQDLFLHVTTKVIDQKMTKTTSKTLSLSFTSLFLGSIDTLIPLMDTHFPELGLKR 360

Query: 361 IDCTEMSWIQSVLFFADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWT 420
             CTEM+WIQSVLFFAD  T++PLE+LM+R LP   S F AKSDYVTSPISQ+GLEGLW 
Sbjct: 361 YQCTEMNWIQSVLFFADFSTKSPLEILMQR-LPSIRSSFIAKSDYVTSPISQSGLEGLWA 420

Query: 421 KLLEEEKSELILTPYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIRE 480
           KLLE + SELI TPYGGKMSQIS+ +TPFPHRKGSIFG+QYLATWD++NE E+HL WIRE
Sbjct: 421 KLLENDNSELIFTPYGGKMSQISELETPFPHRKGSIFGVQYLATWDDSNENERHLRWIRE 480

Query: 481 VYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKV 540
           VY+YMEPYVS +PR  YLNYRDLDLGRNYGRNTSYE+AKVWGLKYF  NFERLVKVKTK 
Sbjct: 481 VYSYMEPYVSMAPRGTYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFKGNFERLVKVKTKF 540

Query: 541 DPSNFFWNEQSIPLIYHYEDDTRIAKVYSGLDFEIVQER 569
           DP NFFWNEQSIPL+Y+ ED+ RI +VYS LD   VQER
Sbjct: 541 DPLNFFWNEQSIPLLYNCEDEARICEVYSDLDSANVQER 556

BLAST of Clc03G08850 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 525.4 bits (1352), Expect = 5.7e-149
Identity = 267/539 (49.54%), Postives = 362/539 (67.16%), Query Frame = 0

Query: 12  LFCVTLIWL---CSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRN 71
           LF VTL+ +    S  +LQ++F++C    S     I  S       +  F   L+ + +N
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 71

Query: 72  LRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKSYD 131
           LR+LT ++PKP+F+  P +E+HVQAA+VCA+K  L +R+RSGGHDYE             
Sbjct: 72  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYE------------- 131

Query: 132 VFEPGLSYVS-SQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTHGF 191
               GLS+V+  + PF+++DL  L  + +D+++ +A    GAT+GE+YYRI +KS THGF
Sbjct: 132 ----GLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGF 191

Query: 192 PAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIR 251
           PAG C ++G+GGH+ GG +G++ RK+GL ADNV+DA+IVD NG+I+DR +MGED+FWAIR
Sbjct: 192 PAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIR 251

Query: 252 GGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIFLHV- 311
           GGGG SFGVIL+WK+KLV VP+ VTVF V +TLEQ  T++  KW+ IA KL  D+F+ V 
Sbjct: 252 GGGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVI 311

Query: 312 --TTKVTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFF 371
                 T  P  +TI +S+ + FLG   RL+ +M+  FPELGL   DCTEMSWI+SV++ 
Sbjct: 312 ISPASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI 371

Query: 372 ADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPY 431
           A  P     E L+     L ++ F AKSD+V  PI   GLEGLW + LEE+    I  PY
Sbjct: 372 AGFPNSAAPEALL-AGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPY 431

Query: 432 GGKMSQISKSQTPFPHRKGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPR 491
           GG MS+IS+S+ PFPHR G++F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR
Sbjct: 432 GGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPR 491

Query: 492 AAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 543
            AY+NYRDLDLG N G       A+ WG KY+  NFERLVK+K + DP NFF +EQS+P
Sbjct: 492 QAYVNYRDLDLGTNEGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Clc03G08850 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 524.6 bits (1350), Expect = 9.7e-149
Identity = 265/542 (48.89%), Postives = 368/542 (67.90%), Query Frame = 0

Query: 9   LFLLFCVTLIWLCSCKSL-QENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIR 68
           + +LF V  IW  S  S   E F +C  S S  KH  PIS  ++ + + S+SS+LQ +IR
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANIR 65

Query: 69  NLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKSY 128
           NLRF TT++PKP  ++   HESHVQAAI C ++  LQ+++RSGGHDY+            
Sbjct: 66  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYD------------ 125

Query: 129 DVFEPGLSYVS-SQAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTHG 188
                GLSYV+ S  PF V+D+ NL S+ +D+ ++TA V TGA LGE+YY I +KS T  
Sbjct: 126 -----GLSYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLA 185

Query: 189 FPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAI 248
           +PAG CPTVGVGGHISGGG+G + RKYGL  DN IDA++VD NG+I+DR  MGEDL+WAI
Sbjct: 186 YPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAI 245

Query: 249 RGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIFLHV 308
            GGGG S+GV+L++K+ LV VP  VTVF + RTLEQ AT +  +WQ +A KL  ++F+  
Sbjct: 246 NGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRT 305

Query: 309 TTKVTN--FPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFF 368
              V N    S+KT+R +F ++FLG    L+ ++   FPELGL   DCTE SWIQSVLF+
Sbjct: 306 VIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFW 365

Query: 369 ADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPY 428
            ++   +   +L++R+ P+  ++   KSDYV  PIS+ GLE +W K++E E   +   PY
Sbjct: 366 TNIQVGSSETLLLQRNQPV--NYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPY 425

Query: 429 GGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRA 488
           GG+M +IS + TPFP+R G+++ IQY A W +   T++++   R++Y +M P+VSK+PR 
Sbjct: 426 GGEMGRISSTVTPFPYRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQ 485

Query: 489 AYLNYRDLDLGRN--YGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 545
           ++ NYRD+DLG N   G+ +SY + K +G KYF+ NFERLVK+KT+VD  NFF NEQSIP
Sbjct: 486 SFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 526

BLAST of Clc03G08850 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 522.7 bits (1345), Expect = 3.7e-148
Identity = 269/541 (49.72%), Postives = 367/541 (67.84%), Query Frame = 0

Query: 13  FCVTLIWL------CSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSI 72
           F VTL+ L       S  ++Q++F+ C    S +   +  S   +   ++SF   L+ S 
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 71

Query: 73  RNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKS 132
           +NLR+L  ++PKP F+  P +E+HVQAA++CA+K  L +R+RSGGHDYE           
Sbjct: 72  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYE----------- 131

Query: 133 YDVFEPGLSYVSS-QAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTH 192
                 GLSYVS  +  F+++DL  L  I +DIE+ +A V  GA++GE+YYRI +KS  H
Sbjct: 132 ------GLSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIH 191

Query: 193 GFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWA 252
           GFPAG C ++G+GGHI GG +G++ RK+GL ADNV+DA+IVD +G+I++R +MGED+FWA
Sbjct: 192 GFPAGLCTSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWA 251

Query: 253 IRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIAHKLHQDIFLH 312
           IRGGGG SFGVIL+WK+KLV VP IVTVF V RTLEQ  T+L  KWQ +A KL +D+F+ 
Sbjct: 252 IRGGGGGSFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIR 311

Query: 313 VTTKVTNFPSK---KTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVL 372
           V  + T+   K   +TI  S+   FLG   RL+ +M+  FP+LGL   DC E SWI+SV+
Sbjct: 312 VIIQPTSKTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVM 371

Query: 373 FFADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILT 432
           + A  P+  P E L++    L +++F AKSDYV  PI   GLEGLW KLLEE+    I  
Sbjct: 372 YIAGFPSTAPSEALLD-GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWN 431

Query: 433 PYGGKMSQISKSQTPFPHRKGSIFGIQYLATWDNANETE-KHLSWIREVYTYMEPYVSKS 492
           PYGG M++I +++TPFPHR G++F IQ+L  W +   +E KH+ W+RE+Y+YME YVSKS
Sbjct: 432 PYGGMMAKIPETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKS 491

Query: 493 PRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSI 543
           PR+AY+NYRDLDLG N G+ +    A+ WG +YF  NFERLV++K K DP NFF +EQSI
Sbjct: 492 PRSAYVNYRDLDLGMN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSI 530

BLAST of Clc03G08850 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 521.2 bits (1341), Expect = 1.1e-147
Identity = 262/540 (48.52%), Postives = 364/540 (67.41%), Query Frame = 0

Query: 7   IYLFLLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSI 66
           +  F L+ +T     S  SLQ+ F+ C    ++    +  +    T   + FS +L+ + 
Sbjct: 14  VLYFSLYTITP--TSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTA 73

Query: 67  RNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKS 126
           +NLRFL  + PKP F+ +P HESHVQA+I+C++K  + +RVRSGGHDYE           
Sbjct: 74  QNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYE----------- 133

Query: 127 YDVFEPGLSYVSS-QAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTH 186
                 GLSYVS    PFI++DL  +  + I+I+  +A V +GAT+GELYYRIA+KS  H
Sbjct: 134 ------GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVH 193

Query: 187 GFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWA 246
           GFPAG C ++G+GGHI+GG +G++ RKYGL ADNV+DAKIVD NG+++DR +MGED FWA
Sbjct: 194 GFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWA 253

Query: 247 IRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQG-ATQLFQKWQTIAHKLHQDIFL 306
           IRGG G SFG+IL+WK+KLV VP  VTVF V +TL+Q    ++  KWQ +A KL +++F+
Sbjct: 254 IRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFI 313

Query: 307 HVTTKVTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFF 366
            V   V      KT+  S+ +LFLG    L+ +MK  FPELGL   DC EMSW++S+ + 
Sbjct: 314 RVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYI 373

Query: 367 ADLPTETPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPY 426
           +  PT TP  VL++   P  +  F AKSD+V +PI ++GL+G++ KLL+E+   +I  PY
Sbjct: 374 SGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPY 433

Query: 427 GGKMSQISKSQTPFPHRKGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPR 486
           GG M++I +SQ PFPHRKG +F +QY+ +W D+     +H++WIR++Y+YM PYVS +PR
Sbjct: 434 GGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPR 493

Query: 487 AAYLNYRDLDLGRN-YGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 543
            AY+NYRDLDLGRN     T  +QA+VWG  YF +NF RL+ +K KVDP NFF +EQSIP
Sbjct: 494 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of Clc03G08850 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 519.2 bits (1336), Expect = 4.1e-147
Identity = 265/540 (49.07%), Postives = 362/540 (67.04%), Query Frame = 0

Query: 11  LLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRNLR 70
           L F    I L S  SLQ++F++C    +  + ++  +       ++ F+ +L+ + +N R
Sbjct: 18  LYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLESTAQNQR 77

Query: 71  FLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEVLHEIITEPKSYDVF 130
           +LT T PKP F+  P HESHVQA+++C++K  +  RVRSGGHDYE               
Sbjct: 78  YLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYE--------------- 137

Query: 131 EPGLSYVSS-QAPFIVIDLINLSSIIIDIETETALVGTGATLGELYYRIAKKSPTHGFPA 190
             G+SYVS  + PF++IDL  L  I +DI+  +A V  GAT+GELYYRIA+KS  HGFPA
Sbjct: 138 --GVSYVSQIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPA 197

Query: 191 GSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGG 250
           G  P++G+GGHI+GG +G+L RKYGLAADNV+DAKIVD NG+++DR SMGEDLFWAIRGG
Sbjct: 198 GVYPSLGIGGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGG 257

Query: 251 GGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGAT-QLFQKWQTIAHKLHQDIFLHVTT 310
            G SFG+ILSWK+KLV VP  +TVF V +T EQ  + ++  KWQ IA  L  ++FL V  
Sbjct: 258 SGGSFGIILSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFF 317

Query: 311 KVTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLP 370
            V+   + KT+ +++   FLG    L+ +MK  FPELGL   DC EMSWI S+++ +  P
Sbjct: 318 TVSGNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFP 377

Query: 371 TE--TPLEVLMERSLPLSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGG 430
           T    P+E+L++   P+ + +F  KSD+   PI   GLEG++ KLLEE+ + +I TPYGG
Sbjct: 378 TNPPPPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGG 437

Query: 431 KMSQISKSQTPFPHRKGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAA 490
           KM +I +S+ PFPHR G+ F IQY  +W D+     +   WIRE+Y YM PYVS +PR A
Sbjct: 438 KMDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQA 497

Query: 491 YLNYRDLDLGRNYGRNTS-YEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLI 545
           Y+NYRDLDLG+N   + S + +AK+WG  YF DNF RLV++K+KVDP NFF +EQSIP +
Sbjct: 498 YVNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895849.14.6e-28688.79berberine bridge enzyme-like 8 [Benincasa hispida][more]
XP_008445632.17.7e-28187.04PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0047127.... [more]
KAE8653305.18.2e-27584.62hypothetical protein Csa_023319 [Cucumis sativus][more]
XP_004135724.18.2e-27584.62berberine bridge enzyme-like 13 [Cucumis sativus][more]
XP_022140178.11.5e-25278.26berberine bridge enzyme-like 8 [Momordica charantia][more]
Match NameE-valueIdentityDescription
O647438.0e-14849.54Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV... [more]
Q9SA851.4e-14748.89Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2... [more]
Q93ZA35.2e-14749.72Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=... [more]
Q9FKU81.5e-14648.52Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... [more]
Q9FKU95.7e-14649.07Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7TYX13.7e-28187.04Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BE113.7e-28187.04flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC10348858... [more]
A0A6J1CHF07.3e-25378.26berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE... [more]
A0A6J1JUS41.3e-24977.26berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111489931 PE=3... [more]
A0A6J1GQY44.7e-24475.99berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111456716 PE... [more]
Match NameE-valueIdentityDescription
AT2G34790.15.7e-14949.54FAD-binding Berberine family protein [more]
AT1G30700.19.7e-14948.89FAD-binding Berberine family protein [more]
AT1G30760.13.7e-14849.72FAD-binding Berberine family protein [more]
AT5G44400.11.1e-14748.52FAD-binding Berberine family protein [more]
AT5G44390.14.1e-14749.07FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 141..233
e-value: 7.5E-16
score: 58.1
NoneNo IPR availableGENE3D3.40.462.20coord: 270..481
e-value: 2.8E-171
score: 572.1
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 27..543
NoneNo IPR availablePANTHERPTHR32448:SF121FAD-BINDING BERBERINE FAMILY PROTEINcoord: 27..543
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..23
score: 6.0
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 485..542
e-value: 3.3E-22
score: 78.3
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 156..542
e-value: 2.8E-171
score: 572.1
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 24..151
e-value: 1.8E-34
score: 119.8
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 75..267
score: 17.676689
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 27..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc03G08850.2Clc03G08850.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding