Clc03G06140 (gene) Watermelon (cordophanus) v2

Overview
NameClc03G06140
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Descriptionpolyphenol oxidase, chloroplastic-like
LocationClcChr03: 5857923 .. 5860832 (-)
RNA-Seq ExpressionClc03G06140
SyntenyClc03G06140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCCCATCAATAAAAATAATTCAACATTTTAGTTGAAATTAAGATAAATCATTTTTATATTTTTTTGGGTTATAAATACAACACAAACGAGTAGCATTAGAAATAAACCAAGCTTTTGAATCAAATAATCAAACAATGGCTTCTCTATCCCATCCACCAACTCTCACCCCATTTCTCCCATCATCCAATCCCGTTCTCCGAAGAACAATAACCTTTCCCATTCCTCGAATCTCATGTAACGCCGCCAATGGCAATGACCAAAATACCCCCAAACCCAAAATTGATAGAAGAGACGTTCTCATTGGCCTTACCGGTCTCTACGGAGCTTCAACTCTCTCCCAAAGCTCCACTTTTGCTCTCGCCGCTCCAGTCCAAACCCCCCACATCAGCAAGTGTGGCCCACCAGACCTGCCACCTGGCGCCGACCAAACCAACTGCTGCCCGCCGCCCGCCACCTCAATCCTCGACTTCCAGCCACCGCCACCCGGAAAATTACGGGTCCGGCCAGCCGCCCATTTAGCCGATCAAAACTACATAGAAAAATACAAGAGAGCCGTTGAGCTAATGAAAGCACTGCCCGATGACGACCCACGTAATTTTACACAACAAGCAAATGTTCACTGTGCTTATTGTAATGGAGCGTATGACCAAGTGGGGTTTCCCGTTGAAGTTCAAGTTCATAATTCATGGCTGTTTTTTCCATTCCATAGATTTTATCTTTACTTTCATGAGAAGATATTGGGAGAGTTGATTGGCGATCCAAGTTTCGCGTTGCCGTTTTGGAATTATGATGCTCCCGGAGGAATGAAAATGCCTGAGATGTACGCGGAGGAGAAATCATCGCTTTATGATGAACTTCGTAACCAAAATCATTTGCCGCCGAGATTGGTGAATTTGGATTTCGGTGGAGATGATCCTACGGTTACCGATGATGCGCAGATAAGGAGTAATCTTAGTATTATGTATCGGCAGATGGTTTCTAATAGTAGTACGCCATCGCTGTTTTTTGGTAGCGCTTATAAAGCGGGTGATGAACCTTCACCAGGTAAATTAATTAATTCAAAAAAAACTTCTTTTTAATACTTAGATCTTAGTTTTATTCATGGTTTTGTATTTTAAACTTTGAAATTCCAGAACTAATTATTAAAATAATTGTTAGTTAATAAAGCATCGCGTAAAAGAAAGAAGAGAAATACTGTTTTAATATGTAATTTAATCGTATGTAAATATTTTAACGACAAACTTTTGGTTGAGACATAAATTCTGGAAAAAATGTTTGAATTTTGAATTCTCACTCGTTGAACTCAATATTGTTTGTCACATTATTTGGAGATTTTTCTTCTAATAATTTTTCTTACTCCACTTGGTAGCAAAAGCAACTTAGTCATATCACAAGCTAGATACCAACCTTTTTTTCTTTTCTTGATTTGTATTTGAAAGGAAAATTCAATGAGTTTCATAATAATCATTGAAAATCATGCTCCATGACAATCACAAAACATAAATTACTTTTCTCTCAAACTTTCAAAATTTCAAAGAATGAAAACAAAAATGTGAGGACAATCTATGTTGTATCATCCTTGATGTCACCTAACAACTATTTAATTTAAATTGGAAATCAACTATTTTTATGCATCTCACCAAATTATCTCATCATAATATGACATGTGGTAAATTATTTTTATTTTTATTTTTAAAATATTTTGATTATTTTTTTGTTGCGAGTGATGGAAACATACATATATATATATATAGGTGTATCTTAAGATGCACGGAAAATACTATCCTAAAAAAAGTGACATGGCAATTTTTTTTTTTTTTGAATGGTATGACAGGAGGTGGTTCAGTGGAGAATATTCCTCACGGTCCGGTTCACGTATGGTGCGGCGACAACAACGAACCCAACTTCGAGAACATGGGAAACTTCTACTCCGCCGCCAGAGACCCCATTTTCTTCGCCCACCACTCCAACATCGACCGTTTTTGGTCCATCTGGAAGACCCTTGGCGGCAAACGACAGGACCTTCGAAACCCCGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAAAAATCCCAACCCGTCCGAGTCCGAGTCCGCGACTGTCTCGACACCAAAACTCTCGGCTACGTCTACCAAGACGTTGACCTCCCATGGCTCCGAACCCGTCCCACTCCTCGCCGAGCCCAAAGATCATCAACCGTCGCGAATAACAACATCCTCCCCTTCGGCATCGGCCCCGCCATGGCTGCCACCAACCCCGGCCCCTCCTCCAGCCGCGTCAGACCGGCTAAGATCAAGTTCCCTATCACTCTGAAGTCAGCGGTGAGCGTGGAAGTGAAGAGACCGAAGAAATCGAGGAGCAAGAAGGAGAAAGAGGATGAAGAGGAGATTTTGGTAATTTATGGAATTGGATTCGATCCTAACCAAGCCATAAAATTCGATGTTTTCATTAACGATGAAGATGACAGGGAAATTAGACCGGATAATACTGAGTTTGCGGGCAGCTTCGTGAACGTGCCACATAAGCACGGGCACAAGAAGAAGGTGAAGACCGGACTTAGGTTGGGAATAACCGAATTGCTTGAAGATTTGGAAGCTGATAATGACGACAGCGTCATCGTCACTCTCATTCCTAGGCTTGGAGCTGGGTTGTTCTCCATTGATGGAATCAAGATCGAATTTGATAAATAATTTAACTCTTGGGATTATTAATAATTAGGGTTTAATTAATGTATAATTAATTAAACCTTTATTTATTTGCACTGATTTTGTGTATGTAATAATTTGGGATATGTGTTTTTGAAGACTTTTTATAAGTCCCTGTTTTGTTGAATAATTATTTCGAACAACTTTATTTGATGGGGGAGACTACTATTTTCATGTATATATATTTTTTTTCTTTTAA

mRNA sequence

TTTCCCATCAATAAAAATAATTCAACATTTTAGTTGAAATTAAGATAAATCATTTTTATATTTTTTTGGGTTATAAATACAACACAAACGAGTAGCATTAGAAATAAACCAAGCTTTTGAATCAAATAATCAAACAATGGCTTCTCTATCCCATCCACCAACTCTCACCCCATTTCTCCCATCATCCAATCCCGTTCTCCGAAGAACAATAACCTTTCCCATTCCTCGAATCTCATGTAACGCCGCCAATGGCAATGACCAAAATACCCCCAAACCCAAAATTGATAGAAGAGACGTTCTCATTGGCCTTACCGGTCTCTACGGAGCTTCAACTCTCTCCCAAAGCTCCACTTTTGCTCTCGCCGCTCCAGTCCAAACCCCCCACATCAGCAAGTGTGGCCCACCAGACCTGCCACCTGGCGCCGACCAAACCAACTGCTGCCCGCCGCCCGCCACCTCAATCCTCGACTTCCAGCCACCGCCACCCGGAAAATTACGGGTCCGGCCAGCCGCCCATTTAGCCGATCAAAACTACATAGAAAAATACAAGAGAGCCGTTGAGCTAATGAAAGCACTGCCCGATGACGACCCACGTAATTTTACACAACAAGCAAATGTTCACTGTGCTTATTGTAATGGAGCGTATGACCAAGTGGGGTTTCCCGTTGAAGTTCAAGTTCATAATTCATGGCTGTTTTTTCCATTCCATAGATTTTATCTTTACTTTCATGAGAAGATATTGGGAGAGTTGATTGGCGATCCAAGTTTCGCGTTGCCGTTTTGGAATTATGATGCTCCCGGAGGAATGAAAATGCCTGAGATGTACGCGGAGGAGAAATCATCGCTTTATGATGAACTTCGTAACCAAAATCATTTGCCGCCGAGATTGGTGAATTTGGATTTCGGTGGAGATGATCCTACGGTTACCGATGATGCGCAGATAAGGAGTAATCTTAGTATTATGTATCGGCAGATGGTTTCTAATAGTAGTACGCCATCGCTGTTTTTTGGTAGCGCTTATAAAGCGGGTGATGAACCTTCACCAGGAGGTGGTTCAGTGGAGAATATTCCTCACGGTCCGGTTCACGTATGGTGCGGCGACAACAACGAACCCAACTTCGAGAACATGGGAAACTTCTACTCCGCCGCCAGAGACCCCATTTTCTTCGCCCACCACTCCAACATCGACCGTTTTTGGTCCATCTGGAAGACCCTTGGCGGCAAACGACAGGACCTTCGAAACCCCGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAAAAATCCCAACCCGTCCGAGTCCGAGTCCGCGACTGTCTCGACACCAAAACTCTCGGCTACGTCTACCAAGACGTTGACCTCCCATGGCTCCGAACCCGTCCCACTCCTCGCCGAGCCCAAAGATCATCAACCGTCGCGAATAACAACATCCTCCCCTTCGGCATCGGCCCCGCCATGGCTGCCACCAACCCCGGCCCCTCCTCCAGCCGCGTCAGACCGGCTAAGATCAAGTTCCCTATCACTCTGAAGTCAGCGGTGAGCGTGGAAGTGAAGAGACCGAAGAAATCGAGGAGCAAGAAGGAGAAAGAGGATGAAGAGGAGATTTTGGTAATTTATGGAATTGGATTCGATCCTAACCAAGCCATAAAATTCGATGTTTTCATTAACGATGAAGATGACAGGGAAATTAGACCGGATAATACTGAGTTTGCGGGCAGCTTCGTGAACGTGCCACATAAGCACGGGCACAAGAAGAAGGTGAAGACCGGACTTAGGTTGGGAATAACCGAATTGCTTGAAGATTTGGAAGCTGATAATGACGACAGCGTCATCGTCACTCTCATTCCTAGGCTTGGAGCTGGGTTGTTCTCCATTGATGGAATCAAGATCGAATTTGATAAATAATTTAACTCTTGGGATTATTAATAATTAGGGTTTAATTAATGTATAATTAATTAAACCTTTATTTATTTGCACTGATTTTGTGTATGTAATAATTTGGGATATGTGTTTTTGAAGACTTTTTATAAGTCCCTGTTTTGTTGAATAATTATTTCGAACAACTTTATTTGATGGGGGAGACTACTATTTTCATGTATATATATTTTTTTTCTTTTAA

Coding sequence (CDS)

ATGGCTTCTCTATCCCATCCACCAACTCTCACCCCATTTCTCCCATCATCCAATCCCGTTCTCCGAAGAACAATAACCTTTCCCATTCCTCGAATCTCATGTAACGCCGCCAATGGCAATGACCAAAATACCCCCAAACCCAAAATTGATAGAAGAGACGTTCTCATTGGCCTTACCGGTCTCTACGGAGCTTCAACTCTCTCCCAAAGCTCCACTTTTGCTCTCGCCGCTCCAGTCCAAACCCCCCACATCAGCAAGTGTGGCCCACCAGACCTGCCACCTGGCGCCGACCAAACCAACTGCTGCCCGCCGCCCGCCACCTCAATCCTCGACTTCCAGCCACCGCCACCCGGAAAATTACGGGTCCGGCCAGCCGCCCATTTAGCCGATCAAAACTACATAGAAAAATACAAGAGAGCCGTTGAGCTAATGAAAGCACTGCCCGATGACGACCCACGTAATTTTACACAACAAGCAAATGTTCACTGTGCTTATTGTAATGGAGCGTATGACCAAGTGGGGTTTCCCGTTGAAGTTCAAGTTCATAATTCATGGCTGTTTTTTCCATTCCATAGATTTTATCTTTACTTTCATGAGAAGATATTGGGAGAGTTGATTGGCGATCCAAGTTTCGCGTTGCCGTTTTGGAATTATGATGCTCCCGGAGGAATGAAAATGCCTGAGATGTACGCGGAGGAGAAATCATCGCTTTATGATGAACTTCGTAACCAAAATCATTTGCCGCCGAGATTGGTGAATTTGGATTTCGGTGGAGATGATCCTACGGTTACCGATGATGCGCAGATAAGGAGTAATCTTAGTATTATGTATCGGCAGATGGTTTCTAATAGTAGTACGCCATCGCTGTTTTTTGGTAGCGCTTATAAAGCGGGTGATGAACCTTCACCAGGAGGTGGTTCAGTGGAGAATATTCCTCACGGTCCGGTTCACGTATGGTGCGGCGACAACAACGAACCCAACTTCGAGAACATGGGAAACTTCTACTCCGCCGCCAGAGACCCCATTTTCTTCGCCCACCACTCCAACATCGACCGTTTTTGGTCCATCTGGAAGACCCTTGGCGGCAAACGACAGGACCTTCGAAACCCCGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAAAAATCCCAACCCGTCCGAGTCCGAGTCCGCGACTGTCTCGACACCAAAACTCTCGGCTACGTCTACCAAGACGTTGACCTCCCATGGCTCCGAACCCGTCCCACTCCTCGCCGAGCCCAAAGATCATCAACCGTCGCGAATAACAACATCCTCCCCTTCGGCATCGGCCCCGCCATGGCTGCCACCAACCCCGGCCCCTCCTCCAGCCGCGTCAGACCGGCTAAGATCAAGTTCCCTATCACTCTGAAGTCAGCGGTGAGCGTGGAAGTGAAGAGACCGAAGAAATCGAGGAGCAAGAAGGAGAAAGAGGATGAAGAGGAGATTTTGGTAATTTATGGAATTGGATTCGATCCTAACCAAGCCATAAAATTCGATGTTTTCATTAACGATGAAGATGACAGGGAAATTAGACCGGATAATACTGAGTTTGCGGGCAGCTTCGTGAACGTGCCACATAAGCACGGGCACAAGAAGAAGGTGAAGACCGGACTTAGGTTGGGAATAACCGAATTGCTTGAAGATTTGGAAGCTGATAATGACGACAGCGTCATCGTCACTCTCATTCCTAGGCTTGGAGCTGGGTTGTTCTCCATTGATGGAATCAAGATCGAATTTGATAAATAA

Protein sequence

MASLSHPPTLTPFLPSSNPVLRRTITFPIPRISCNAANGNDQNTPKPKIDRRDVLIGLTGLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPPPGKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFPVEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSSLYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYKAGDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSIWKTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRPTPRRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRPKKSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHKHGHKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK
Homology
BLAST of Clc03G06140 vs. NCBI nr
Match: XP_004146644.1 (polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64656.1 hypothetical protein Csa_013940 [Cucumis sativus])

HSP 1 Score: 1037.7 bits (2682), Expect = 3.8e-299
Identity = 498/590 (84.41%), Postives = 544/590 (92.20%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSNPVLRRTITFPI-PRISCNAANGNDQNTPKPKIDRRDVLIGLT 60
           MASLSHPP+LT       P LR+T T  I PRISC A N NDQN P PKIDRRD+LIGLT
Sbjct: 1   MASLSHPPSLT-----LTPFLRKTKTLLITPRISCKATNHNDQNFPNPKIDRRDILIGLT 60

Query: 61  GLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPPPGK 120
           GLYG+STLSQS+ FALAAP++TP+ISKCGPPDLPPGAD TNCCPPPAT+ILDFQPPPP K
Sbjct: 61  GLYGSSTLSQSTHFALAAPIETPNISKCGPPDLPPGADATNCCPPPATTILDFQPPPPSK 120

Query: 121 LRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFPVEV 180
           LRVRPAAHLAD+NYIEKYK+AVELMKALP+DDPR+FTQQANVHCAYCNGAYDQVGFPVEV
Sbjct: 121 LRVRPAAHLADRNYIEKYKKAVELMKALPEDDPRSFTQQANVHCAYCNGAYDQVGFPVEV 180

Query: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSSLYD 240
           QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAP GMKMP MYAEEKSSLYD
Sbjct: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPDGMKMPGMYAEEKSSLYD 240

Query: 241 ELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYKAGD 300
           ELRN+NHLPPRLV+LDFGG DP + DDAQI+SNLSIMYRQMVS S TPSLFFGSAY AGD
Sbjct: 241 ELRNENHLPPRLVDLDFGGVDPNIGDDAQIKSNLSIMYRQMVSGSRTPSLFFGSAYVAGD 300

Query: 301 EPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSIWKT 360
           EPSPGGGSVENIPHGPVHVWCGD ++PNFENMGNFYSAARDPIFFAHHSNIDR WS+WKT
Sbjct: 301 EPSPGGGSVENIPHGPVHVWCGDTDQPNFENMGNFYSAARDPIFFAHHSNIDRLWSVWKT 360

Query: 361 LGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRPTPR 420
           LGG+RQ+L+NPEWL+ASFIFY+EK+QP+RVRVRDCLD+K+LGY+YQ VDLPWL+TRPTPR
Sbjct: 361 LGGRRQNLQNPEWLNASFIFYDEKAQPIRVRVRDCLDSKSLGYIYQPVDLPWLQTRPTPR 420

Query: 421 RAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRPKKSR 480
           RAQ++STVA      FGI PA+AA   GPSSSRV+PA IKFPIT+KS VSVEVKRPKKSR
Sbjct: 421 RAQKTSTVA------FGIAPALAA--GGPSSSRVKPANIKFPITVKSPVSVEVKRPKKSR 480

Query: 481 SKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHKHGHK 540
           SKKEKE+EEE+LVIYGIGFDPN  IKFDVFINDEDD+EI+PDNTEFAGSFVNVPHKHGHK
Sbjct: 481 SKKEKEEEEEVLVIYGIGFDPNNGIKFDVFINDEDDKEIKPDNTEFAGSFVNVPHKHGHK 540

Query: 541 KKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK 590
           KKVKTGLR+GITE LEDLEADNDDS+IVT++PR+GAGLFSIDG+KIEFDK
Sbjct: 541 KKVKTGLRVGITEELEDLEADNDDSIIVTILPRIGAGLFSIDGLKIEFDK 577

BLAST of Clc03G06140 vs. NCBI nr
Match: XP_038903169.1 (polyphenol oxidase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1030.4 bits (2663), Expect = 6.1e-297
Identity = 501/594 (84.34%), Postives = 543/594 (91.41%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSNPVLRRTITFP---IPR-ISCNAANGNDQNTPKPKIDRRDVLI 60
           MASLSHPP+LT + PSSN  L +T T P    PR ISCN  N NDQNTPKPKIDRRD+L+
Sbjct: 1   MASLSHPPSLTTYFPSSNSFLPKTKTLPNNISPRIISCNTVNHNDQNTPKPKIDRRDILV 60

Query: 61  GLTGLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPP 120
           GL+GLYGASTL ++S FALAAP+QTP+ISKCGP DLPPGAD TNCCPPPAT ILDFQPP 
Sbjct: 61  GLSGLYGASTLYKNSNFALAAPIQTPNISKCGPADLPPGADSTNCCPPPATRILDFQPPA 120

Query: 121 PGKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFP 180
             KLRVRPAAHL D+NYIEKYK+AVELMKALP++DPR+FTQQANVHCAYCNGAYDQVGFP
Sbjct: 121 QEKLRVRPAAHLVDKNYIEKYKKAVELMKALPENDPRSFTQQANVHCAYCNGAYDQVGFP 180

Query: 181 VEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSS 240
           VEVQVHNSWLFFPFHR YLYFHEKILGELIGDP+FALPFWNYDAPGGM+MPEMYAEEKSS
Sbjct: 181 VEVQVHNSWLFFPFHRLYLYFHEKILGELIGDPNFALPFWNYDAPGGMRMPEMYAEEKSS 240

Query: 241 LYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYK 300
           LYDELRN+NH+PPRLV+LDFG  DP + DDAQIRSNLSIMYRQMVSNS TPSLFFGSAY+
Sbjct: 241 LYDELRNENHVPPRLVDLDFGRVDPNIGDDAQIRSNLSIMYRQMVSNSRTPSLFFGSAYR 300

Query: 301 AGDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSI 360
           AGDEPSPGGGS+ENIPHGPVHVWCGDNNEPN ENMGNFYSAARDPIF+AHHSNIDR WS+
Sbjct: 301 AGDEPSPGGGSIENIPHGPVHVWCGDNNEPNGENMGNFYSAARDPIFYAHHSNIDRLWSV 360

Query: 361 WKTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRP 420
           WKTLGG+RQDL+NPEWL+ SFIFYNEK++ VRVRVR  LD+K LGYVYQDVD+PWL+TRP
Sbjct: 361 WKTLGGRRQDLQNPEWLNTSFIFYNEKAEAVRVRVRQSLDSKILGYVYQDVDVPWLQTRP 420

Query: 421 TPRRAQRSSTVANNN-ILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRP 480
           TPRRAQ+  TVANNN ILPF IGPA+AA   GPSSSRVRPA IKFPIT+KSAVSVEVKRP
Sbjct: 421 TPRRAQK--TVANNNIILPFSIGPALAA---GPSSSRVRPANIKFPITVKSAVSVEVKRP 480

Query: 481 KKSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHK 540
           KKSRSKKEKE+EEEILVIYGIGFDPNQ IKFDVFINDEDD+EIRPDNTEFAGSFVNVPHK
Sbjct: 481 KKSRSKKEKEEEEEILVIYGIGFDPNQGIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHK 540

Query: 541 HGHKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK 590
            GHKK VKTGLRLGITE+LEDLEAD+DDSV VT++PRLGAGLFSIDGIKI  DK
Sbjct: 541 LGHKKTVKTGLRLGITEILEDLEADDDDSVRVTIVPRLGAGLFSIDGIKIVCDK 589

BLAST of Clc03G06140 vs. NCBI nr
Match: XP_008442274.1 (PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056968.1 polyphenol oxidase [Cucumis melo var. makuwa] >TYK26394.1 polyphenol oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.2e-291
Identity = 489/591 (82.74%), Postives = 535/591 (90.52%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSNPVLRRTITFPI-PRISCNAANGNDQNTPKPKIDRRDVLIGLT 60
           MA LSHPP+LT       P L +T T  I  RISC A+N NDQNT  PKIDRRD+LIGLT
Sbjct: 1   MAFLSHPPSLT-----LTPFLHKTKTISITSRISCKASNHNDQNTSNPKIDRRDILIGLT 60

Query: 61  GLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPPPGK 120
           GLYG+STLSQS  FALAAP+QTP+ISKCGPPDLPPGAD TNCCPPPAT+ILDFQPPPP K
Sbjct: 61  GLYGSSTLSQSPHFALAAPIQTPNISKCGPPDLPPGADPTNCCPPPATTILDFQPPPPTK 120

Query: 121 LRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFPVEV 180
           LRVRPAAHLADQNYIEKYK+A+ELMKALP+DDPR+FTQQANVHCAYCNGAYDQ+GFPVEV
Sbjct: 121 LRVRPAAHLADQNYIEKYKKAIELMKALPEDDPRSFTQQANVHCAYCNGAYDQIGFPVEV 180

Query: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSSLYD 240
           QVHNSWLFFPFHRFYLYFHEKILGELIGD SFALPFWNYDAPGGMKMP MYAEEKSSLYD
Sbjct: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDSSFALPFWNYDAPGGMKMPRMYAEEKSSLYD 240

Query: 241 ELRNQNHLPPRLVNLDFGG-DDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYKAG 300
           ELRN+NHLPPRLV+LDFGG DDP + DDAQIRSNLSIMYRQMVS S TPSLFFGSAY  G
Sbjct: 241 ELRNENHLPPRLVDLDFGGVDDPNIGDDAQIRSNLSIMYRQMVSGSRTPSLFFGSAYVEG 300

Query: 301 DEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSIWK 360
           DEPSPGGGSVENIPHGPVHVWCGDN++PNFENMGNFYSAARDPIFFAHHSN+DR WS+WK
Sbjct: 301 DEPSPGGGSVENIPHGPVHVWCGDNDQPNFENMGNFYSAARDPIFFAHHSNVDRLWSVWK 360

Query: 361 TLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRPTP 420
           TLGG+R+DL+NPEWL+ASFIFYNEK+QPVRV VRDCLD+K+LGY YQ VDLPWL+TRPTP
Sbjct: 361 TLGGRRKDLQNPEWLNASFIFYNEKAQPVRVHVRDCLDSKSLGYTYQPVDLPWLQTRPTP 420

Query: 421 RRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRPKKS 480
           RRAQ+ +TVA      FGI  A+AA   GPSSSRV+PA IKFPIT+KS VSVEVKRPKKS
Sbjct: 421 RRAQK-TTVA------FGIASALAA---GPSSSRVKPANIKFPITVKSPVSVEVKRPKKS 480

Query: 481 RSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHKHGH 540
           RSKKEKE+EEE+LVIYGIGFDPN  IKFDVFINDEDD+EI+PDNTEFAGSFVNVPHK  H
Sbjct: 481 RSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFINDEDDKEIKPDNTEFAGSFVNVPHKQAH 540

Query: 541 KKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK 590
           KKKVKTGLR+GITE+LEDLEADNDDS+IVT++P++GAGLFSI G+KIE D+
Sbjct: 541 KKKVKTGLRVGITEVLEDLEADNDDSIIVTIVPKIGAGLFSIGGLKIELDE 576

BLAST of Clc03G06140 vs. NCBI nr
Match: APO14262.1 (polyphenol oxidase 3 [Luffa aegyptiaca])

HSP 1 Score: 970.7 bits (2508), Expect = 5.7e-279
Identity = 471/594 (79.29%), Postives = 523/594 (88.05%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSN---PVLRRTITF--PIPRISCNAANGNDQNTPKPKIDRRDVL 60
           ++S +   T  PFLP++    P LRRT  F    PRI C+A+NG   +     IDRRD+L
Sbjct: 8   LSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQNH-----IDRRDIL 67

Query: 61  IGLTGLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPP 120
           IGL GLYGASTLSQ S  A AAPVQTP+ISKCG PDLPPGAD  NCCPPP T+++DF+P 
Sbjct: 68  IGLGGLYGASTLSQGS-LAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFRPQ 127

Query: 121 PPGKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGF 180
            PGKLRVRPAAHLADQNY+EKYKRAVELMKALPDDDPR+F QQANVHCAYCNGAYDQVGF
Sbjct: 128 APGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQVGF 187

Query: 181 PVEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKS 240
           PVEVQVHNSWLFFPFHR+YL+FHEKILGELIGDPSFALPFWNYDAPGGMKMP MYA+EKS
Sbjct: 188 PVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEKS 247

Query: 241 SLYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAY 300
           SLYDELRN+NHLPP LV+LDFGG DPT+ D+AQIRSNLSIMYRQMVSNS TPSLFFG+AY
Sbjct: 248 SLYDELRNRNHLPPTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFFGNAY 307

Query: 301 KAGDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWS 360
            AGDEPSPGGG VENIPHGPVH+WCGDNNEPNFENMGNFYSAARDPIF+AHHSNIDR WS
Sbjct: 308 LAGDEPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDRLWS 367

Query: 361 IWKTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTR 420
           +WKTLGG+RQDL+N +WL+ASFIFY+EK+Q VRVRVRDCLD++ LGYVYQDV LPWL +R
Sbjct: 368 VWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWLESR 427

Query: 421 PTPRRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRP 480
           PTPRRA R+     +  LPFG+GPA+AA  PGPSSSR+RPAKIKFPITL S +S+EVKRP
Sbjct: 428 PTPRRALRAQKAVAH--LPFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISMEVKRP 487

Query: 481 KKSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHK 540
           K+SRSKKEK++EEEI+VIYGI FD N+AIKFDVFINDEDD+EIRPDNTEFAGSFVNVPHK
Sbjct: 488 KRSRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHK 547

Query: 541 HGHKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK 590
           HGHKK VKTGLRLGITELLEDL+AD+DDSV VTL+PR G GL SI GIKIEFDK
Sbjct: 548 HGHKKNVKTGLRLGITELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEFDK 593

BLAST of Clc03G06140 vs. NCBI nr
Match: KAG6584007.1 (hypothetical protein SDJN03_19939, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 904.8 bits (2337), Expect = 3.8e-259
Identity = 445/590 (75.42%), Postives = 493/590 (83.56%), Query Frame = 0

Query: 1   MASLSHPPTLT-PFLPSSNPVLRRTITFPIPRISCNAANGNDQNTPKPKIDRRDVLIGL- 60
           MASL  P   T  F PSS+    + +     RI C+AA+G        KIDRRDVLIG+ 
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADG--------KIDRRDVLIGIG 60

Query: 61  -TGLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPPP 120
             GLYGAS  ++ STFALAAPVQTP+ISKCGPPDLPPGAD TNCCPPP TSI+DF PP P
Sbjct: 61  AGGLYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAP 120

Query: 121 GKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFPV 180
           GKLRVRPAAHLADQ+YI+KYKRAVELMK LP+DDPR+F QQANVHCAYCNG YDQVGFPV
Sbjct: 121 GKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPV 180

Query: 181 EVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSSL 240
           EVQVHNSWLFFPFHR+YLYF+EKILGELI DP+FALPFWNYDAPGGMKMP MYA++ SSL
Sbjct: 181 EVQVHNSWLFFPFHRYYLYFYEKILGELIEDPTFALPFWNYDAPGGMKMPAMYADQNSSL 240

Query: 241 YDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYKA 300
           YD+LRNQNHLPP  ++LDF G D  + D+AQIRSNL IMYRQMVSNS TP LFFGSAY A
Sbjct: 241 YDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVA 300

Query: 301 GDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSIW 360
           GDEPSPGGGSVENIPH  VH WCGDNNEPN ENMGNFYSAARDPIF++HHSNIDR WS+W
Sbjct: 301 GDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVW 360

Query: 361 KTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRPT 420
           KTLGG+RQDL+ PEWLDASFIFYNEK+Q VRVRVRDCLDTKTLGY+YQDV+LPWL TRPT
Sbjct: 361 KTLGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKTLGYIYQDVELPWLETRPT 420

Query: 421 PRRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRPKK 480
           PRRA R+    ++N+                 S RV+PA+IKFP+T+KSAVS EVKR KK
Sbjct: 421 PRRALRAQKSVDSNV----------------PSRRVKPARIKFPVTVKSAVSTEVKRAKK 480

Query: 481 SRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHKHG 540
           SRSKKEKE+EEEILVI GI FDPN AIK DV+INDEDD EIR DNTEFAGSFVNVPHKHG
Sbjct: 481 SRSKKEKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHG 540

Query: 541 HKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEF 588
           HKKKVKTGLRLGI+E+LEDLEAD+DDS+IVT+IPRLGAGL SI  I IE+
Sbjct: 541 HKKKVKTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITIEY 566

BLAST of Clc03G06140 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 1.4e-208
Identity = 365/604 (60.43%), Postives = 448/604 (74.17%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLP----------SSNPVLRRTITFP----IPRISCNAANGNDQNTPK 60
           M SLS P   TP +P          S N      +T P      ++SC A N +  +  +
Sbjct: 1   MTSLSPPVVTTPTVPNPATKPLSPFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQ 60

Query: 61  PKIDRRDVLIGLTGLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPA 120
            K+DRR+VL+GL GLYG + +  +  FA A P+  P +SKCGP DLP GA  TNCCPPP+
Sbjct: 61  SKLDRRNVLLGLGGLYGVAGMG-TDPFAFAKPIAPPDVSKCGPADLPQGAVPTNCCPPPS 120

Query: 121 TSILDFQPPPPGKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYC 180
           T I+DF+ P P KLR+RP AH  DQ Y +KY +A+ELMKALPDDDPR+F QQA VHCAYC
Sbjct: 121 TKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAYC 180

Query: 181 NGAYDQVGFP-VEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMK 240
           +GAYDQVGFP +E+Q+HNSWLFFPFHR+YLYF EKILG+LI DP+FALPFWN+D+P GM 
Sbjct: 181 DGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMP 240

Query: 241 MPEMYAEEKSSLYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSS 300
           +P +YA+ KS LYD+LR+ NH PP LV+LD+ G +  V+ +  I +NL IMYRQMVSNS 
Sbjct: 241 LPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSK 300

Query: 301 TPSLFFGSAYKAGDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFA 360
              LFFG+ Y+AGDEP PGGGS+E  PH PVH+W GDN +PNFE+MGNFYSA RDPIFFA
Sbjct: 301 NAKLFFGNPYRAGDEPDPGGGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIFFA 360

Query: 361 HHSNIDRFWSIWKTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQ 420
           HHSN+DR WSIWKTLGGKR DL + +WLD+ F+FYNE ++ VRV+VRDCL+TK LGYVYQ
Sbjct: 361 HHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQ 420

Query: 421 DVDLPWLRTRPTPRRAQRS-STVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITL 480
           DVD+PWL ++PTPRRA+ + S VA        +G A AA     SSS+V  A  +FPI+L
Sbjct: 421 DVDIPWLSSKPTPRRAKVALSKVAKK------LGVAHAAV---ASSSKV-VAGTEFPISL 480

Query: 481 KSAVSVEVKRPK-KSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNT 540
            S +S  VKRPK K RSKK KEDEEEILVI GI FD + A+KFDV++ND DD    PD T
Sbjct: 481 GSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDKT 540

Query: 541 EFAGSFVNVPHKHGHKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGI 588
           EFAGSFV+VPH H HKKK+ T LRLG+T+LLE++EA++DDSV+VTL+P+ GA    I GI
Sbjct: 541 EFAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGA--VKIGGI 591

BLAST of Clc03G06140 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 5.0e-177
Identity = 323/620 (52.10%), Postives = 416/620 (67.10%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSNPVLRRTITFPI------------PRISCNAANGNDQNTPKPK 60
           M S+S    ++    SSN  +  +  +P             P+     ++ N+QN PK +
Sbjct: 1   MTSISALSFISTINVSSNSKISHSSVYPFLQKQHQSSKLRKPKRQVTCSSNNNQNNPKEE 60

Query: 61  ID-------RRDVLIGLTGLYGASTLSQSSTFALAAPVQTPHISKCGPP-DLPPGA--DQ 120
            +       RR+VLIGL G+YG    + S   ALA+P+  P +SKC PP DLP G     
Sbjct: 61  QELSNIVGHRRNVLIGLGGIYGTLATNPS---ALASPISPPDLSKCVPPSDLPSGTTPPN 120

Query: 121 TNCCPPPATSILDFQPPPPGKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQ 180
            NCCPP +T I DF+ P    LRVR AAHL D  ++EKYK+A ELMKALP +DPRNFTQQ
Sbjct: 121 INCCPPYSTKITDFKFPSNQPLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQ 180

Query: 181 ANVHCAYCNGAYDQVGFP-VEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWN 240
           AN+HCAYC+GAY Q+GFP +++QVH SWLFFPFHR+YLYF+E+ILG LI DP+FALPFWN
Sbjct: 181 ANIHCAYCDGAYSQIGFPDLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWN 240

Query: 241 YDAPGGMKMPEMYAEEKSSLYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMY 300
           YDAP GM++P +YA++ S LYDELRN +H PP L++L+F      +  +  I++NLSIMY
Sbjct: 241 YDAPDGMQLPTIYADKASPLYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMY 300

Query: 301 RQMVSNSSTPSLFFGSAYKAGDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSA 360
           RQ+ SN  T  LF G+ Y+AGD    G GS+EN+PH PVH W GDN + N E+MG FYSA
Sbjct: 301 RQVYSNGKTSRLFLGNPYRAGDAEPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSA 360

Query: 361 ARDPIFFAHHSNIDRFWSIWKTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDT 420
           ARDPIF++HHSN+DR W IWKTLGGK+ D  + +WL++ F+FY+E    VRV V+D LD 
Sbjct: 361 ARDPIFYSHHSNVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDI 420

Query: 421 KTLGYVYQDVDLPWLRTRPTPRRAQRSSTV---ANNNILPFGIGPAMAATNPGPSSSRVR 480
             LGY YQDV +PW + +P PRR +    V    N+  L              P+   VR
Sbjct: 421 DKLGYAYQDVPIPWEKAKPVPRRTKVQKLVEVEVNDGNL-----------RKSPTIFLVR 480

Query: 481 ---PAK-IKFPITLKSAVSVEVKRPKKSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFI 540
              P K + FP+ L + VS  VKRPKK RSKKEKE+EEE+LVI GI F  N AIKFDV+I
Sbjct: 481 QQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYI 540

Query: 541 NDEDDREIRPDNTEFAGSFVNVPHK-HGHK-KKVKTGLRLGITELLEDLEADNDDSVIVT 589
           NDEDD+ +   NTEFAGSFVN+PH  HGHK KK+ T LRLGIT+LLEDL  + DD+++VT
Sbjct: 541 NDEDDK-VGAGNTEFAGSFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVT 600

BLAST of Clc03G06140 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 1.9e-176
Identity = 318/594 (53.54%), Postives = 407/594 (68.52%), Query Frame = 0

Query: 7   PPTLTPFLPSSNPVLR-RTITFPIPRISCNAANGND--------QNTPKPKIDRRDVLIG 66
           PP+      S  PV R R+  F   ++SCN+ANG+         + T   K+DRR+VL+G
Sbjct: 24  PPSPLFQRASHVPVARNRSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVLLG 83

Query: 67  LTGLYGAS-TLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPP 126
           + GLYGA+  L  +   A  AP+Q P ISKCG   +P G   TNCCPP  T I+DFQ P 
Sbjct: 84  IGGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPS 143

Query: 127 PGK-LRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGF 186
            G  +R RPAAHL  + Y+ KYK+A+EL KALPDDDPR+F QQANVHC YC GAYDQVG+
Sbjct: 144 SGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVGY 203

Query: 187 -PVEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEK 246
             +E+QVH SWLF PFHR+YLYF+E+IL +LI DP+FALP+W +D P GM MP +YA   
Sbjct: 204 TDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSP 263

Query: 247 SSLYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSA 306
           SSLYDE RN  HLPP +++LD+ G +PT+ DD     NL+IMY+Q+VS ++TP LF G  
Sbjct: 264 SSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYP 323

Query: 307 YKAGDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFW 366
           Y+AGD   PG G++E+ PH  VH W G  ++P+ E+MGNFY+A RDPIFF HH+N+DR W
Sbjct: 324 YRAGDAIDPGAGTLEHAPHNIVHKWTGLADKPS-EDMGNFYTAGRDPIFFGHHANVDRMW 383

Query: 367 SIWKTLGGK-RQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLR 426
           +IWKT+GGK R+D  + +WLDA+F+FY+E  Q V+V+V DC+DT  L Y YQD+ +PWL 
Sbjct: 384 NIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWL- 443

Query: 427 TRPTPRRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVK 486
             P   +A+  +T  ++       G A AA  P  + S +      FP  L S + VEV 
Sbjct: 444 --PKNTKAKAKTTTKSSK-----SGVAKAAELPKTTISSIG----DFPKALNSVIRVEVP 503

Query: 487 RPKKSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVP 546
           RPKKSRSKKEKEDEEE+L+I GI  D    +KFDV+INDED    RP N+EFAGSFVNVP
Sbjct: 504 RPKKSRSKKEKEDEEEVLLIKGIELDRENFVKFDVYINDEDYSVSRPKNSEFAGSFVNVP 563

Query: 547 HKHGHKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEF 588
           HKH  + K KT LR  I ELLEDL A++D+SVIVT++PR G    +I GI+IEF
Sbjct: 564 HKHMKEMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIEF 604

BLAST of Clc03G06140 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 9.4e-168
Identity = 315/605 (52.07%), Postives = 399/605 (65.95%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSNPVLRRTITFPIPRISCNAANGN-DQNTPKPK---IDRRDVLI 60
           MASLS    L    P S P   +     + R+SC A+N N D+   + K   IDRR++LI
Sbjct: 1   MASLSSQSKLLA-TPYSFPYHTKPSRVSLRRVSCKASNDNKDKPNDQEKTFSIDRRNMLI 60

Query: 61  GLTGLYGASTLSQSSTFALAAPVQTPHI-SKCGPP-DLPPGADQTNCCPPPATSILDFQ- 120
           GL GLYGAS +  S+   LAAP+Q P +   C PP DL  G +   CCPP     +DFQ 
Sbjct: 61  GLGGLYGASNVFPSNQSTLAAPIQPPVLPDSCHPPEDLAEGVN-VLCCPPDVKDPIDFQM 120

Query: 121 PPPPGKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQV 180
           P  P +LR+RPAAHLAD  YIEKYK+A+  MKALP +DPR+F QQAN+HCAYCNGAYDQV
Sbjct: 121 PSNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAYCNGAYDQV 180

Query: 181 GFP-VEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAE 240
           GFP V +QVH+SWLF PFHR+YLYF+E+ILG LI DP+FA+PFWN+DAP GM MP M+ +
Sbjct: 181 GFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMHMPHMFID 240

Query: 241 EKSSLYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFG 300
             S LYD  RN  H P  +V+LDF   +    +  QI +NLSIMY+Q+V  +    LF G
Sbjct: 241 PNSPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSIMYKQVV-RAKKARLFHG 300

Query: 301 SAYKAGDEPSPGG-GSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNID 360
              +AG  P   G GS+E  PHG +H+W GD  + NFENMGNFYSA RDP+F+AHH+N+D
Sbjct: 301 RPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNFYSAGRDPLFYAHHANVD 360

Query: 361 RFWSIWKTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPW 420
           R W IWK   G R+D +  +WL+A F+ ++E +QPVRV VRD LD + LGY YQ+VD+PW
Sbjct: 361 RMWYIWKNSLG-RKDYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLGYAYQEVDIPW 420

Query: 421 LRTRPTPRRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKI-----KFPITLKS 480
           + ++P PR+A                       NP P+  R +         KFP+TL S
Sbjct: 421 INSKPKPRKA-----------------------NPWPNRLRSKATTTTKLINKFPLTLDS 480

Query: 481 AVSVEVKRPKKSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFA 540
            VS EVKRPKKSRSK EKEDEEE+LVI  I  +P   +KFDV+INDED+     D TEFA
Sbjct: 481 TVSFEVKRPKKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDEDPSAADQTEFA 540

Query: 541 GSFVNVPHKHGH----KKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDG 588
           GSFVNVPH H H     K+  T L +GI+E+L++L+ D DDS++VTL+PR+G+G  +I G
Sbjct: 541 GSFVNVPHFHRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGG 578

BLAST of Clc03G06140 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 586.3 bits (1510), Expect = 4.0e-166
Identity = 304/599 (50.75%), Postives = 405/599 (67.61%), Query Frame = 0

Query: 3   SLSHPPTLTPFLPSSNPV----LRRTITFPIPRISCNAANGNDQNTPKPKIDRRDVLIGL 62
           SLS   T++  LP  + +     +RT  F   ++SCNA NG+ Q    PK+DRRDVL+GL
Sbjct: 19  SLSSSATISSPLPKPSQIHIATAKRTHHF---KVSCNAPNGDSQ----PKLDRRDVLLGL 78

Query: 63  TGLYGASTLSQSSTFALAAPVQTPHISKC--GPPDLPPGADQTNCCPPPATSILDFQPP- 122
            GL GA++L  ++  A A P+  P ISKC   P DLPP A   NCCPP AT+++ ++ P 
Sbjct: 79  GGLAGAASLI-NNPLAFAEPIHAPEISKCVVPPKDLPPDAIVDNCCPPLATNVIPYKVPK 138

Query: 123 -PPGKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVG 182
             P  +++RPA H  D+ YI K+++A+ LMK LP DDPRNF QQA VHCAYCNG Y Q  
Sbjct: 139 TSPSAMKIRPAIHRMDKEYIAKFEKAIRLMKELPADDPRNFYQQALVHCAYCNGGYVQTD 198

Query: 183 FP-VEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEE 242
           +P  E+QVHNSWLFFPFHR+YLYF+E+ILG+LIGDP+F LPFWN+D P GM +P+ +  +
Sbjct: 199 YPDKEIQVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGMLIPQYFRNQ 258

Query: 243 KSSLYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGS 302
            S LYDE RNQ+HL P +++L + G D  VTD  +I +NL++MY+ MV+N+ T  LF G 
Sbjct: 259 NSPLYDENRNQSHL-PLVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAGTAELFLGK 318

Query: 303 AYKAGDEP-SPGGGSVENIPHGPVHVWCGD--NNEPNFENMGNFYSAARDPIFFAHHSNI 362
            YKAGD+P + GGGS+ENIPH PVH W GD      N E+MGNFYSA RD +F+ HHSN+
Sbjct: 319 PYKAGDDPVNKGGGSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDILFYCHHSNV 378

Query: 363 DRFWSIWKTLGGK--RQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVD 422
           DR W+IW+ LGGK  R+D  + +WLDA+FIFY+E  Q VRVRV D LD + LGY Y+  +
Sbjct: 379 DRMWTIWQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKLGYKYEFTN 438

Query: 423 LPWLRTRPTPRRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAV 482
           LPWL ++P P + +                  +AA +  P  + V      FP+TL   V
Sbjct: 439 LPWLNSKPLPTKKK----------------TGLAARSKAPFVTDV------FPLTLDKVV 498

Query: 483 SVEVKRPKKSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGS 542
            V+V RPKKSRSK+EKE EEEIL I GI    +Q  KFDV++NDED+ E   +  E+AGS
Sbjct: 499 QVKVPRPKKSRSKEEKEAEEEILEIQGIEVAIDQYAKFDVYLNDEDEPEAGKEKAEYAGS 558

Query: 543 FVNVPHKHGHKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEF 588
           F ++PHKH   KK++T L LG+ E LEDL A++DD+V+VTL P++G G+ +++ IKI +
Sbjct: 559 FAHLPHKHTGSKKIRTSLSLGLNEPLEDLGAEDDDAVLVTLAPKVGGGVVTVENIKIVY 586

BLAST of Clc03G06140 vs. ExPASy TrEMBL
Match: A0A0A0LXD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 1.8e-299
Identity = 498/590 (84.41%), Postives = 544/590 (92.20%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSNPVLRRTITFPI-PRISCNAANGNDQNTPKPKIDRRDVLIGLT 60
           MASLSHPP+LT       P LR+T T  I PRISC A N NDQN P PKIDRRD+LIGLT
Sbjct: 1   MASLSHPPSLT-----LTPFLRKTKTLLITPRISCKATNHNDQNFPNPKIDRRDILIGLT 60

Query: 61  GLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPPPGK 120
           GLYG+STLSQS+ FALAAP++TP+ISKCGPPDLPPGAD TNCCPPPAT+ILDFQPPPP K
Sbjct: 61  GLYGSSTLSQSTHFALAAPIETPNISKCGPPDLPPGADATNCCPPPATTILDFQPPPPSK 120

Query: 121 LRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFPVEV 180
           LRVRPAAHLAD+NYIEKYK+AVELMKALP+DDPR+FTQQANVHCAYCNGAYDQVGFPVEV
Sbjct: 121 LRVRPAAHLADRNYIEKYKKAVELMKALPEDDPRSFTQQANVHCAYCNGAYDQVGFPVEV 180

Query: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSSLYD 240
           QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAP GMKMP MYAEEKSSLYD
Sbjct: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPDGMKMPGMYAEEKSSLYD 240

Query: 241 ELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYKAGD 300
           ELRN+NHLPPRLV+LDFGG DP + DDAQI+SNLSIMYRQMVS S TPSLFFGSAY AGD
Sbjct: 241 ELRNENHLPPRLVDLDFGGVDPNIGDDAQIKSNLSIMYRQMVSGSRTPSLFFGSAYVAGD 300

Query: 301 EPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSIWKT 360
           EPSPGGGSVENIPHGPVHVWCGD ++PNFENMGNFYSAARDPIFFAHHSNIDR WS+WKT
Sbjct: 301 EPSPGGGSVENIPHGPVHVWCGDTDQPNFENMGNFYSAARDPIFFAHHSNIDRLWSVWKT 360

Query: 361 LGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRPTPR 420
           LGG+RQ+L+NPEWL+ASFIFY+EK+QP+RVRVRDCLD+K+LGY+YQ VDLPWL+TRPTPR
Sbjct: 361 LGGRRQNLQNPEWLNASFIFYDEKAQPIRVRVRDCLDSKSLGYIYQPVDLPWLQTRPTPR 420

Query: 421 RAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRPKKSR 480
           RAQ++STVA      FGI PA+AA   GPSSSRV+PA IKFPIT+KS VSVEVKRPKKSR
Sbjct: 421 RAQKTSTVA------FGIAPALAA--GGPSSSRVKPANIKFPITVKSPVSVEVKRPKKSR 480

Query: 481 SKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHKHGHK 540
           SKKEKE+EEE+LVIYGIGFDPN  IKFDVFINDEDD+EI+PDNTEFAGSFVNVPHKHGHK
Sbjct: 481 SKKEKEEEEEVLVIYGIGFDPNNGIKFDVFINDEDDKEIKPDNTEFAGSFVNVPHKHGHK 540

Query: 541 KKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK 590
           KKVKTGLR+GITE LEDLEADNDDS+IVT++PR+GAGLFSIDG+KIEFDK
Sbjct: 541 KKVKTGLRVGITEELEDLEADNDDSIIVTILPRIGAGLFSIDGLKIEFDK 577

BLAST of Clc03G06140 vs. ExPASy TrEMBL
Match: A0A5D3DS75 (Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00570 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.1e-291
Identity = 489/591 (82.74%), Postives = 535/591 (90.52%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSNPVLRRTITFPI-PRISCNAANGNDQNTPKPKIDRRDVLIGLT 60
           MA LSHPP+LT       P L +T T  I  RISC A+N NDQNT  PKIDRRD+LIGLT
Sbjct: 1   MAFLSHPPSLT-----LTPFLHKTKTISITSRISCKASNHNDQNTSNPKIDRRDILIGLT 60

Query: 61  GLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPPPGK 120
           GLYG+STLSQS  FALAAP+QTP+ISKCGPPDLPPGAD TNCCPPPAT+ILDFQPPPP K
Sbjct: 61  GLYGSSTLSQSPHFALAAPIQTPNISKCGPPDLPPGADPTNCCPPPATTILDFQPPPPTK 120

Query: 121 LRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFPVEV 180
           LRVRPAAHLADQNYIEKYK+A+ELMKALP+DDPR+FTQQANVHCAYCNGAYDQ+GFPVEV
Sbjct: 121 LRVRPAAHLADQNYIEKYKKAIELMKALPEDDPRSFTQQANVHCAYCNGAYDQIGFPVEV 180

Query: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSSLYD 240
           QVHNSWLFFPFHRFYLYFHEKILGELIGD SFALPFWNYDAPGGMKMP MYAEEKSSLYD
Sbjct: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDSSFALPFWNYDAPGGMKMPRMYAEEKSSLYD 240

Query: 241 ELRNQNHLPPRLVNLDFGG-DDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYKAG 300
           ELRN+NHLPPRLV+LDFGG DDP + DDAQIRSNLSIMYRQMVS S TPSLFFGSAY  G
Sbjct: 241 ELRNENHLPPRLVDLDFGGVDDPNIGDDAQIRSNLSIMYRQMVSGSRTPSLFFGSAYVEG 300

Query: 301 DEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSIWK 360
           DEPSPGGGSVENIPHGPVHVWCGDN++PNFENMGNFYSAARDPIFFAHHSN+DR WS+WK
Sbjct: 301 DEPSPGGGSVENIPHGPVHVWCGDNDQPNFENMGNFYSAARDPIFFAHHSNVDRLWSVWK 360

Query: 361 TLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRPTP 420
           TLGG+R+DL+NPEWL+ASFIFYNEK+QPVRV VRDCLD+K+LGY YQ VDLPWL+TRPTP
Sbjct: 361 TLGGRRKDLQNPEWLNASFIFYNEKAQPVRVHVRDCLDSKSLGYTYQPVDLPWLQTRPTP 420

Query: 421 RRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRPKKS 480
           RRAQ+ +TVA      FGI  A+AA   GPSSSRV+PA IKFPIT+KS VSVEVKRPKKS
Sbjct: 421 RRAQK-TTVA------FGIASALAA---GPSSSRVKPANIKFPITVKSPVSVEVKRPKKS 480

Query: 481 RSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHKHGH 540
           RSKKEKE+EEE+LVIYGIGFDPN  IKFDVFINDEDD+EI+PDNTEFAGSFVNVPHK  H
Sbjct: 481 RSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFINDEDDKEIKPDNTEFAGSFVNVPHKQAH 540

Query: 541 KKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK 590
           KKKVKTGLR+GITE+LEDLEADNDDS+IVT++P++GAGLFSI G+KIE D+
Sbjct: 541 KKKVKTGLRVGITEVLEDLEADNDDSIIVTIVPKIGAGLFSIGGLKIELDE 576

BLAST of Clc03G06140 vs. ExPASy TrEMBL
Match: A0A1S3B632 (polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103486180 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.1e-291
Identity = 489/591 (82.74%), Postives = 535/591 (90.52%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSNPVLRRTITFPI-PRISCNAANGNDQNTPKPKIDRRDVLIGLT 60
           MA LSHPP+LT       P L +T T  I  RISC A+N NDQNT  PKIDRRD+LIGLT
Sbjct: 1   MAFLSHPPSLT-----LTPFLHKTKTISITSRISCKASNHNDQNTSNPKIDRRDILIGLT 60

Query: 61  GLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPPPGK 120
           GLYG+STLSQS  FALAAP+QTP+ISKCGPPDLPPGAD TNCCPPPAT+ILDFQPPPP K
Sbjct: 61  GLYGSSTLSQSPHFALAAPIQTPNISKCGPPDLPPGADPTNCCPPPATTILDFQPPPPTK 120

Query: 121 LRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFPVEV 180
           LRVRPAAHLADQNYIEKYK+A+ELMKALP+DDPR+FTQQANVHCAYCNGAYDQ+GFPVEV
Sbjct: 121 LRVRPAAHLADQNYIEKYKKAIELMKALPEDDPRSFTQQANVHCAYCNGAYDQIGFPVEV 180

Query: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSSLYD 240
           QVHNSWLFFPFHRFYLYFHEKILGELIGD SFALPFWNYDAPGGMKMP MYAEEKSSLYD
Sbjct: 181 QVHNSWLFFPFHRFYLYFHEKILGELIGDSSFALPFWNYDAPGGMKMPRMYAEEKSSLYD 240

Query: 241 ELRNQNHLPPRLVNLDFGG-DDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYKAG 300
           ELRN+NHLPPRLV+LDFGG DDP + DDAQIRSNLSIMYRQMVS S TPSLFFGSAY  G
Sbjct: 241 ELRNENHLPPRLVDLDFGGVDDPNIGDDAQIRSNLSIMYRQMVSGSRTPSLFFGSAYVEG 300

Query: 301 DEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSIWK 360
           DEPSPGGGSVENIPHGPVHVWCGDN++PNFENMGNFYSAARDPIFFAHHSN+DR WS+WK
Sbjct: 301 DEPSPGGGSVENIPHGPVHVWCGDNDQPNFENMGNFYSAARDPIFFAHHSNVDRLWSVWK 360

Query: 361 TLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRPTP 420
           TLGG+R+DL+NPEWL+ASFIFYNEK+QPVRV VRDCLD+K+LGY YQ VDLPWL+TRPTP
Sbjct: 361 TLGGRRKDLQNPEWLNASFIFYNEKAQPVRVHVRDCLDSKSLGYTYQPVDLPWLQTRPTP 420

Query: 421 RRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRPKKS 480
           RRAQ+ +TVA      FGI  A+AA   GPSSSRV+PA IKFPIT+KS VSVEVKRPKKS
Sbjct: 421 RRAQK-TTVA------FGIASALAA---GPSSSRVKPANIKFPITVKSPVSVEVKRPKKS 480

Query: 481 RSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHKHGH 540
           RSKKEKE+EEE+LVIYGIGFDPN  IKFDVFINDEDD+EI+PDNTEFAGSFVNVPHK  H
Sbjct: 481 RSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFINDEDDKEIKPDNTEFAGSFVNVPHKQAH 540

Query: 541 KKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK 590
           KKKVKTGLR+GITE+LEDLEADNDDS+IVT++P++GAGLFSI G+KIE D+
Sbjct: 541 KKKVKTGLRVGITEVLEDLEADNDDSIIVTIVPKIGAGLFSIGGLKIELDE 576

BLAST of Clc03G06140 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.8e-279
Identity = 471/594 (79.29%), Postives = 523/594 (88.05%), Query Frame = 0

Query: 1   MASLSHPPTLTPFLPSSN---PVLRRTITF--PIPRISCNAANGNDQNTPKPKIDRRDVL 60
           ++S +   T  PFLP++    P LRRT  F    PRI C+A+NG   +     IDRRD+L
Sbjct: 8   LSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQNH-----IDRRDIL 67

Query: 61  IGLTGLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPP 120
           IGL GLYGASTLSQ S  A AAPVQTP+ISKCG PDLPPGAD  NCCPPP T+++DF+P 
Sbjct: 68  IGLGGLYGASTLSQGS-LAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFRPQ 127

Query: 121 PPGKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGF 180
            PGKLRVRPAAHLADQNY+EKYKRAVELMKALPDDDPR+F QQANVHCAYCNGAYDQVGF
Sbjct: 128 APGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQVGF 187

Query: 181 PVEVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKS 240
           PVEVQVHNSWLFFPFHR+YL+FHEKILGELIGDPSFALPFWNYDAPGGMKMP MYA+EKS
Sbjct: 188 PVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEKS 247

Query: 241 SLYDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAY 300
           SLYDELRN+NHLPP LV+LDFGG DPT+ D+AQIRSNLSIMYRQMVSNS TPSLFFG+AY
Sbjct: 248 SLYDELRNRNHLPPTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFFGNAY 307

Query: 301 KAGDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWS 360
            AGDEPSPGGG VENIPHGPVH+WCGDNNEPNFENMGNFYSAARDPIF+AHHSNIDR WS
Sbjct: 308 LAGDEPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDRLWS 367

Query: 361 IWKTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTR 420
           +WKTLGG+RQDL+N +WL+ASFIFY+EK+Q VRVRVRDCLD++ LGYVYQDV LPWL +R
Sbjct: 368 VWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWLESR 427

Query: 421 PTPRRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRP 480
           PTPRRA R+     +  LPFG+GPA+AA  PGPSSSR+RPAKIKFPITL S +S+EVKRP
Sbjct: 428 PTPRRALRAQKAVAH--LPFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISMEVKRP 487

Query: 481 KKSRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHK 540
           K+SRSKKEK++EEEI+VIYGI FD N+AIKFDVFINDEDD+EIRPDNTEFAGSFVNVPHK
Sbjct: 488 KRSRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHK 547

Query: 541 HGHKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEFDK 590
           HGHKK VKTGLRLGITELLEDL+AD+DDSV VTL+PR G GL SI GIKIEFDK
Sbjct: 548 HGHKKNVKTGLRLGITELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEFDK 593

BLAST of Clc03G06140 vs. ExPASy TrEMBL
Match: A0A6J1EGP5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434015 PE=3 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 2.1e-258
Identity = 443/590 (75.08%), Postives = 493/590 (83.56%), Query Frame = 0

Query: 1   MASLSHPPTLT-PFLPSSNPVLRRTITFPIPRISCNAANGNDQNTPKPKIDRRDVLIGL- 60
           MASL  P   T  F PSS+    + +     RI C+AA+G        KIDRRDVLIG+ 
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADG--------KIDRRDVLIGIG 60

Query: 61  -TGLYGASTLSQSSTFALAAPVQTPHISKCGPPDLPPGADQTNCCPPPATSILDFQPPPP 120
             GLYGAS+ ++ STFALAAPVQTP+ISKCGPPDLPPGAD TNCCPPP TSI+DF PP P
Sbjct: 61  AGGLYGASSFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAP 120

Query: 121 GKLRVRPAAHLADQNYIEKYKRAVELMKALPDDDPRNFTQQANVHCAYCNGAYDQVGFPV 180
           GKLRVRPAAHLADQ+YI+KYKRAVELMK LP+DDPR+F QQANVHCAYCNG YDQVGFPV
Sbjct: 121 GKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPV 180

Query: 181 EVQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPGGMKMPEMYAEEKSSL 240
           EVQVHNSWLFFPFHR+YLYF+EKILGELIGDP+FALPFWNYDAPGGMKMP MYA++ SSL
Sbjct: 181 EVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSL 240

Query: 241 YDELRNQNHLPPRLVNLDFGGDDPTVTDDAQIRSNLSIMYRQMVSNSSTPSLFFGSAYKA 300
           YD+LRNQNHLPP  ++LDF G D  + D+AQIRSNL IMYRQMVSNS TP LFFGSAY A
Sbjct: 241 YDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVA 300

Query: 301 GDEPSPGGGSVENIPHGPVHVWCGDNNEPNFENMGNFYSAARDPIFFAHHSNIDRFWSIW 360
           GDEPSPGGGSVENIPH  VH WCGDNNEPN ENMGNFYSAARDPIF++HHSNIDR WS+W
Sbjct: 301 GDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVW 360

Query: 361 KTLGGKRQDLRNPEWLDASFIFYNEKSQPVRVRVRDCLDTKTLGYVYQDVDLPWLRTRPT 420
           KTLGG+RQDL+ PEWLDASFIFYNEK+Q VRVRVRDCLDTK LGY+YQDV+L WL TRPT
Sbjct: 361 KTLGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPT 420

Query: 421 PRRAQRSSTVANNNILPFGIGPAMAATNPGPSSSRVRPAKIKFPITLKSAVSVEVKRPKK 480
           PRRA R+    ++N+                 S RV+PA+IKFP+T+KSAVS EVKR KK
Sbjct: 421 PRRALRAQKSVDSNV----------------PSRRVKPARIKFPVTVKSAVSTEVKRAKK 480

Query: 481 SRSKKEKEDEEEILVIYGIGFDPNQAIKFDVFINDEDDREIRPDNTEFAGSFVNVPHKHG 540
           SRSKKEKE+EEEILVI GI FDPN AIK DV+INDEDD EIR DNTEFAGSFVNVPHKHG
Sbjct: 481 SRSKKEKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHG 540

Query: 541 HKKKVKTGLRLGITELLEDLEADNDDSVIVTLIPRLGAGLFSIDGIKIEF 588
           HKKKVKTGLRLGI+E+LEDLEAD+DDS+IVT+IPRLGAGL SI  I +E+
Sbjct: 541 HKKKVKTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITMEY 566

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146644.13.8e-29984.41polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64656.1 hypothetical pro... [more]
XP_038903169.16.1e-29784.34polyphenol oxidase, chloroplastic-like [Benincasa hispida][more]
XP_008442274.12.2e-29182.74PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056968.1 p... [more]
APO14262.15.7e-27979.29polyphenol oxidase 3 [Luffa aegyptiaca][more]
KAG6584007.13.8e-25975.42hypothetical protein SDJN03_19939, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
P433091.4e-20860.43Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q062155.0e-17752.10Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
P433111.9e-17653.54Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q6UIL39.4e-16852.07(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... [more]
Q9MB144.0e-16650.75Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LXD01.8e-29984.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1[more]
A0A5D3DS751.1e-29182.74Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G0... [more]
A0A1S3B6321.1e-29182.74polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103486180 P... [more]
A0A1L5JHU32.8e-27979.29Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1EGP52.1e-25875.08polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 312..323
score: 41.33
coord: 213..218
score: 55.1
coord: 339..357
score: 53.92
coord: 182..199
score: 56.35
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 152..359
e-value: 8.8E-33
score: 114.5
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 340..351
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 182..199
IPR016213Polyphenol oxidasePIRSFPIRSF000290PPO_plantcoord: 3..589
e-value: 3.6E-254
score: 842.9
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 77..413
e-value: 4.7E-121
score: 406.4
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 82..412
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 460..587
e-value: 2.8E-53
score: 179.5
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 366..417
e-value: 9.6E-24
score: 82.9
NoneNo IPR availablePIRSRPIRSR000290-1PIRSR000290-1coord: 77..588
e-value: 2.1E-228
score: 757.0
NoneNo IPR availablePIRSRPIRSR000290-2PIRSR000290-2coord: 77..588
e-value: 2.1E-228
score: 757.0
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 10..588
NoneNo IPR availablePANTHERPTHR11474:SF94POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 10..588

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc03G06140.1Clc03G06140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity