Clc03G03400 (gene) Watermelon (cordophanus) v2

Overview
NameClc03G03400
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionProtein kinase domain-containing protein
LocationClcChr03: 3458597 .. 3462364 (-)
RNA-Seq ExpressionClc03G03400
SyntenyClc03G03400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAAATTTCCTTCTGCGGAAGAATATTTCTTCTCATCTCCTTCTCCTTCCCAAACCCCACAGAAATCTTCTTCAACCATCTTCTCCCTTTCAAGACTCTGCTTCTTTCTGCGAGATCATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCCGAGGCCCCTAAGGTATAATCTACGATCCTCTCTTTCAGATCTGATTTTATTTTGTCTTGATTTTGGTTTTGTAGAGCTAGATCTGCTGGATTTATCTTTGTTTAGGTATGTGTTAGTTTCACACACCGAGGTATAATCTCTGATCAGCGTAGCTTTAGAAGCCTTTCGGTTTGTCGTCGTTGTTTATTATCCTTGCCTTTTCTGATGGCCGATTTACGTTTTGTTTTTAATTTTAATTTCTTTTTCTATCAAGATTCTTGATTTTGTTCTACGAGTCTATCCTATGTTCTTGTAGTTTCTTTACCGATCTGTTGAGTTAACGAGTTTTTATTTTATTTTTAAGCTTGAATGAATTAACTTGTCAGATCAGGTAAGTATTTTCCTTTATTCATTTGGATGTTTATGAGCAACTTTTATCTCTCTTCGTATGGATGATTTTTTCTTATAATTGTTGCTTCAGTCCACGATCTGTTTTAGCTAGGAGTTTCTACGTTTTTTTTTCCACCATCCGTGTCTGTTTACCATGTATCTGGCTTATATTTTGTATATATCCAAGAATTTGCCTCCCATCTGTATTCATACAACAAAATCTTGCAATTTCTTTTGTGTTATGGAAAATGAGGACTTTGGGTGAGGATATTAGGTTTAGGAAGGGCAAACTTTTATATGAATGGGTGACTGTGTGTAGGCCCAGGTAGGAATATGTTGTGGACAAGAGATTGCGAATATTCCAAGCTTTGCATCTACAAGAGCACCATCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCACGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTGTAAATACACTTGACTATGATTAAAGAAACTTTTATGTGCTATAAAATTGATCATCCTAGCATTCTTCTCTTATTAATTAGTTCCTTTGCTTATTTAAATGATGGCTATAATTATTAATTTTTGCTTTGAACTTAAGATCGTATTTAAAAGAGTATTCATCATCTTTACCTTCATCTACTTTTCCCAAAACAGAACTTATCTGCCATTTGATATGTTTTTCAAAATCATTACTTAATTGGAATGGTTTAGGACTAGTGAGATTTAGGGACTTGTCAACTTTTTCCTGATCGTTTCTTTTTCTTACCCAGTCAATTCATTTCTTCTATGGCAGATAAAATACATAGCAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGGTGAGTTTCTTTTTTGTTTTTAGACTCTTTCTTATGACGTTGTTGGCACTCTGTGGCTCTTGTCATCATTGTGGCTATGGTTAAATGGCTTTATTCCTTGCAGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTAAGTATAAGGCTGTTTTATCATGGATTAATTTCCCCGATTCACCACCAAATATGGGGGGTTAAAATTTGTGGGTGAACTTAAATGGTTTTAACAGGTGTTTGAGAAGCTTGGGCCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATGTGTGTAGTATTTTATTGAACCTAGTCCTTTCTTCTTTTCCCATTCTTAGTGTCTTTTGTTTGTTGCAAAACATAACTCCACGTTTTTTCATGTTAAATTGGTTATAAGCATCTGTTGTGTTCCACTTTGTTTATTCACTTTTAACAAAATTGTGTGAGGAGTCAGTTTCTTATCATTGTGTGTTCTGTATCCTAATAATTTCTTTGACCCTTGAAATCCAGTCATGCATGATCTACGAATGATACATACGGACTTGAAGCCAGAGAACATATTGTTGGTCTCCGCAGATTATGTAAAAGTACACGACTACAAGGTACTTTGATCTTGTTTCTGATCTGGTTTCCATTTTGAAATTCTGTTTGATCTTCTTGAGGATGATTGTACGAGCTATTTCAGGTTCTGGTTTCTCATAATATTTTGATCTTTCGTGTCAGAATTTGTCTCGATCACCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCTAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACATATGATCGTCAAGACCAGAATTACATTGTATCAACACGCCATTATAGAGCACCAGAGGTTATTCTTGGTAAGGATTTTTGATCATTTTTCTTTTTTCTTTTTTTCTTTTTTTGTTTTTGTTTTCACGAGTGTTCTGATTTGTGTTGAGTGCATGGCTTGACCATATTTAATTGTGTAGGTCTTGGATGGAGTTTTCCTTGTGACATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGTATGTGCTTCTTTTCTCTGTGAATACGTCCTTTATAGAGGAAGGTTTTGTGTAATTGAATTTTCAATACGAAATGATTTTTAGGGTGAAGCACTGTTTCAAACTCATGAAAACTTGGAGCACTTGGCAATGATGGAGCGAGTGCTCGGTCCATTGCCACAGGAGATGCTGAAGAGAGTAGAGTGAGTATATTCTCACCTTTTTTCCCCCTTGTTTTTAAGCTGTCAAGAAACCAAATGCCGATGTACGTATTTATTTATTTTGTTTTGGTATATCCATAGTCGGCATGCAGAAAAATATGTGAGAAGAGGCAGATTAGATTGGCCAGAAGGTGCAACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGGCTCGTTTACCGGTTTGTATTTTAATGGTGTCTTAAAAGGTTGGATAGGACCTGTTTGGTTTGCAAGTTAACACTAATTATCTCGTGTAAACACGTGCAGAACTTGATAATGCAGCATGTGGACCATTCAGCTGGGGAATTGATTCATCTTGTGCAAGGGTTGCTTCGATACGATCCTACACATAGACTAACGGCTCGTGAGGCTCTAAGGCACCCATTCTTTACAAGGGATCCCTTGAGGAGATAGTAGGCTGTGTTGTCTCATATTTAACTGACTGGCATTTGAGTTTTTAACACTCTTCTCCCATCGTCTTCATGCAACTGTATAGCTGCGCTAGTCTGTTGATTGACGATCGCCATTCCTCTGTGGCTCCAGAAGAGGACTATTTCGCTGTGATGTAAATATATATATGCTTAGTCCAAGCAGAGTTTTGCAATGCTAGCACTAGTTGCTTAATTGCACATGATTCCGAATGTTTGATTAGAAAGATATACACTAACTTATTTTTACGACATTGGGTGTACGTATTTGCAAAATTATCAACGAGGACGGTTGAGATGGTGGTTGACATACATATAACATATGATTATGGCAGAATGTAGCAGGATGTGCTTCTAATTTTCTCTGTTTATTTTGTATTTGTTTAATTAACAACAATGTG

mRNA sequence

CACAAATTTCCTTCTGCGGAAGAATATTTCTTCTCATCTCCTTCTCCTTCCCAAACCCCACAGAAATCTTCTTCAACCATCTTCTCCCTTTCAAGACTCTGCTTCTTTCTGCGAGATCATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCCGAGGCCCCTAAGGTATGTGTTAGTTTCACACACCGAGGTATAATCTCTGATCAGCGTAGCTTTAGAAGCCTTTCGGCCCAGGTAGGAATATGTTGTGGACAAGAGATTGCGAATATTCCAAGCTTTGCATCTACAAGAGCACCATCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCACGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTATAAAATACATAGCAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTGTTTGAGAAGCTTGGGCCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATTCATGCATGATCTACGAATGATACATACGGACTTGAAGCCAGAGAACATATTGTTGGTCTCCGCAGATTATGTAAAAGTACACGACTACAAGAATTTGTCTCGATCACCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCTAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACATATGATCGTCAAGACCAGAATTACATTGTATCAACACGCCATTATAGAGCACCAGAGGTTATTCTTGGTCTTGGATGGAGTTTTCCTTGTGACATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGGTGAAGCACTGTTTCAAACTCATGAAAACTTGGAGCACTTGGCAATGATGGAGCGAGTGCTCGGTCCATTGCCACAGGAGATGCTGAAGAGAGTAGATCGGCATGCAGAAAAATATGTGAGAAGAGGCAGATTAGATTGGCCAGAAGGTGCAACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGGCTCGTTTACCGAACTTGATAATGCAGCATGTGGACCATTCAGCTGGGGAATTGATTCATCTTGTGCAAGGGTTGCTTCGATACGATCCTACACATAGACTAACGGCTCGTGAGGCTCTAAGGCACCCATTCTTTACAAGGGATCCCTTGAGGAGATAGTAGGCTGTGTTGTCTCATATTTAACTGACTGGCATTTGAGTTTTTAACACTCTTCTCCCATCGTCTTCATGCAACTGTATAGCTGCGCTAGTCTGTTGATTGACGATCGCCATTCCTCTGTGGCTCCAGAAGAGGACTATTTCGCTGTGATGTAAATATATATATGCTTAGTCCAAGCAGAGTTTTGCAATGCTAGCACTAGTTGCTTAATTGCACATGATTCCGAATGTTTGATTAGAAAGATATACACTAACTTATTTTTACGACATTGGGTGTACGTATTTGCAAAATTATCAACGAGGACGGTTGAGATGGTGGTTGACATACATATAACATATGATTATGGCAGAATGTAGCAGGATGTGCTTCTAATTTTCTCTGTTTATTTTGTATTTGTTTAATTAACAACAATGTG

Coding sequence (CDS)

ATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCCGAGGCCCCTAAGGTATGTGTTAGTTTCACACACCGAGGTATAATCTCTGATCAGCGTAGCTTTAGAAGCCTTTCGGCCCAGGTAGGAATATGTTGTGGACAAGAGATTGCGAATATTCCAAGCTTTGCATCTACAAGAGCACCATCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCACGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTATAAAATACATAGCAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTGTTTGAGAAGCTTGGGCCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATTCATGCATGATCTACGAATGATACATACGGACTTGAAGCCAGAGAACATATTGTTGGTCTCCGCAGATTATGTAAAAGTACACGACTACAAGAATTTGTCTCGATCACCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCTAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACATATGATCGTCAAGACCAGAATTACATTGTATCAACACGCCATTATAGAGCACCAGAGGTTATTCTTGGTCTTGGATGGAGTTTTCCTTGTGACATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGGTGAAGCACTGTTTCAAACTCATGAAAACTTGGAGCACTTGGCAATGATGGAGCGAGTGCTCGGTCCATTGCCACAGGAGATGCTGAAGAGAGTAGATCGGCATGCAGAAAAATATGTGAGAAGAGGCAGATTAGATTGGCCAGAAGGTGCAACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGGCTCGTTTACCGAACTTGATAATGCAGCATGTGGACCATTCAGCTGGGGAATTGATTCATCTTGTGCAAGGGTTGCTTCGATACGATCCTACACATAGACTAACGGCTCGTGAGGCTCTAAGGCACCCATTCTTTACAAGGGATCCCTTGAGGAGATAG

Protein sequence

MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGICCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR
Homology
BLAST of Clc03G03400 vs. NCBI nr
Match: XP_038894261.1 (serine/threonine-protein kinase AFC2 isoform X1 [Benincasa hispida])

HSP 1 Score: 881.7 bits (2277), Expect = 2.7e-252
Identity = 428/454 (94.27%), Postives = 431/454 (94.93%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY
Sbjct: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRE+KE VAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDREKKETVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVS+DYVKVHDYKNLSRSPRDSSNFKRVP+SSAIKVIDFGSTTYDRQD
Sbjct: 241 MIHTDLKPENILLVSSDYVKVHDYKNLSRSPRDSSNFKRVPRSSAIKVIDFGSTTYDRQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDP HRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPMHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. NCBI nr
Match: XP_008439081.1 (PREDICTED: serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo] >KAA0055279.1 serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo var. makuwa] >TYJ99202.1 serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 879.4 bits (2271), Expect = 1.3e-251
Identity = 426/454 (93.83%), Postives = 430/454 (94.71%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRY
Sbjct: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRE+KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVS DYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD
Sbjct: 241 MIHTDLKPENILLVSGDYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQ+MLK+VDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQDMLKKVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDP HRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPAHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. NCBI nr
Match: XP_023519374.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.5 bits (2266), Expect = 5.1e-251
Identity = 425/454 (93.61%), Postives = 430/454 (94.71%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA K                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIAN+PSFAS+RAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY
Sbjct: 61  CCGQEIANMPSFASSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVSADY+KVHDYKN SRSPRDSSNFKRVPKSS+IKVIDFGSTTYDRQD
Sbjct: 241 MIHTDLKPENILLVSADYMKVHDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDRQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILG+GWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. NCBI nr
Match: XP_023001260.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 876.3 bits (2263), Expect = 1.1e-250
Identity = 425/454 (93.61%), Postives = 429/454 (94.49%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA K                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIAN+PSFAS+RAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY
Sbjct: 61  CCGQEIANMPSFASSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVSADY+KVHDYKN SRSPRDSSNFKRVPKSS IKVIDFGSTTYDRQD
Sbjct: 241 MIHTDLKPENILLVSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILG+GWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. NCBI nr
Match: XP_022923920.1 (serine/threonine-protein kinase AFC2 isoform X1 [Cucurbita moschata] >KAG6584116.1 Serine/threonine-protein kinase AFC2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019705.1 Serine/threonine-protein kinase AFC2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 876.3 bits (2263), Expect = 1.1e-250
Identity = 424/454 (93.39%), Postives = 430/454 (94.71%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA K                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIAN+PSFAS+RAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY
Sbjct: 61  CCGQEIANMPSFASSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVSADY+KVHDYKN SRSPRDSSNFKRVPKSS+IKVIDFGSTTYDR+D
Sbjct: 241 MIHTDLKPENILLVSADYMKVHDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDRED 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILG+GWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. ExPASy Swiss-Prot
Match: P51567 (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=1 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 1.6e-215
Identity = 364/449 (81.07%), Postives = 401/449 (89.31%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A K                     AQVG+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATK---------------------AQVGM 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
            CGQEI NI SFAS+ APSD+SS+ L VKGVARNGSPPWREDDKDGHY+F LG++LT RY
Sbjct: 61  FCGQEIGNISSFASSGAPSDNSSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KI+SKMGEGTFGQVLECWDRERKEMVA+KIVRG++KYR+AAMIEIE+LQQLGKHDKGGNR
Sbjct: 121 KIYSKMGEGTFGQVLECWDRERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLG SLYDFLRKNNYRSFPIDLVR+IG QLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVS+DYVK+ +YK  SR  RD   +KRVPKSSAIKVIDFGSTTY+RQD
Sbjct: 241 MIHTDLKPENILLVSSDYVKIPEYKG-SRLQRDVC-YKRVPKSSAIKVIDFGSTTYERQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           Q YIVSTRHYRAPEVILGLGWS+PCD+WSVGCI+VELC+GEALFQTHENLEHLAMMERVL
Sbjct: 301 QTYIVSTRHYRAPEVILGLGWSYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GP PQ+MLK+VDRH+EKYVRRGRLDWP+GATSRDS+KAV KL RL NLIMQHVDHSAGEL
Sbjct: 361 GPFPQQMLKKVDRHSEKYVRRGRLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTR 450
           I++VQGLLR+DP+ R+TAREALRHPFF R
Sbjct: 421 INMVQGLLRFDPSERITAREALRHPFFAR 425

BLAST of Clc03G03400 vs. ExPASy Swiss-Prot
Match: P51566 (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=2 SV=2)

HSP 1 Score: 637.5 bits (1643), Expect = 1.2e-181
Identity = 311/456 (68.20%), Postives = 359/456 (78.73%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVV------PEAPKVCVSFTHRGIISDQRSFRSL 60
           +E +R +EFPH  +D+RPRKR RL WD        P  P V     + G           
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYG----------- 76

Query: 61  SAQVGICCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGE 120
                          P FAS   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+
Sbjct: 77  ---------------PEFASGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGD 136

Query: 121 NLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKH 180
            LT RY+I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +H
Sbjct: 137 TLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRH 196

Query: 181 DKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVA 240
           D GG+RCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA
Sbjct: 197 DVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVA 256

Query: 241 FMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGST 300
           +MHDLR+IHTDLKPENILLVS++Y+K+ DYK LSR  +D S FK +PKSSAIK+IDFGST
Sbjct: 257 YMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGST 316

Query: 301 TYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLA 360
           T++ QD NYIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLA
Sbjct: 317 TFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLA 376

Query: 361 MMERVLGPLPQEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSIKAVQKLARLPNLIMQHV 420
           MMERVLGPLP  M+ R DR +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHV
Sbjct: 377 MMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHV 436

Query: 421 DHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTR 450
           DHSAG+LI L+QGLLRYDPT R  AREAL HPFFTR
Sbjct: 437 DHSAGDLIDLLQGLLRYDPTERFKAREALNHPFFTR 445

BLAST of Clc03G03400 vs. ExPASy Swiss-Prot
Match: P51568 (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=1 SV=2)

HSP 1 Score: 512.3 bits (1318), Expect = 5.6e-144
Identity = 244/388 (62.89%), Postives = 307/388 (79.12%), Query Frame = 0

Query: 64  QEIANIPSFASTRAPSDHSSNPLFVKGVARNG---SPPWREDDKDGHYMFALGENLTSRY 123
           + +   P      AP++  +    +KG   +G   SPP R+DD+DGHY+F+L +NLT RY
Sbjct: 12  ERVRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSLRDNLTPRY 71

Query: 124 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 183
           KI SKMGEGTFG+VLECWDR+ KE VAIKI+R I+KYRDAAMIEI+VLQ+L K DKG  R
Sbjct: 72  KILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTR 131

Query: 184 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 243
           CVQ++NWFDYRNHICIVFEKLGPSL+DFL++N Y +FP+ LVRD G QLLE VA+MH+L+
Sbjct: 132 CVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLESVAYMHELQ 191

Query: 244 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 303
           ++HTDLKPENILLVS++ VK+ D K   RS  + ++F+ +PKSSAIK+IDFGST  D + 
Sbjct: 192 LVHTDLKPENILLVSSENVKLPDNK---RSAANETHFRCLPKSSAIKLIDFGSTVCDNRI 251

Query: 304 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 363
            + IV TRHYR+PEVILGLGWS+ CD+WS+GCIL ELC+GEALFQTH+NLEHLAMMER L
Sbjct: 252 HHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMMERAL 311

Query: 364 GPLPQEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGE 423
           GPLP+ M ++  R AEKY RRG RL+WPEGA SR+SI+AV++L RL +++ +HVD++   
Sbjct: 312 GPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDMVSKHVDNTRSR 371

Query: 424 LIHLVQGLLRYDPTHRLTAREALRHPFF 448
              L+ GLL YDP+ RLTA EAL HPFF
Sbjct: 372 FADLLYGLLAYDPSERLTANEALDHPFF 396

BLAST of Clc03G03400 vs. ExPASy Swiss-Prot
Match: P49760 (Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.2e-91
Identity = 170/355 (47.89%), Postives = 240/355 (67.61%), Query Frame = 0

Query: 101 EDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRER-KEMVAIKIVRGIRKYRD 160
           EDD +GH ++ +G+ L  RY+I S +GEGTFG+V++C D  R    VA+KI++ + KY++
Sbjct: 144 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKE 203

Query: 161 AAMIEIEVLQQLGKHDKGG-NRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFP 220
           AA +EI VL+++ + D    N CVQ+ +WFDY  H+CI FE LG S +DFL+ NNY  +P
Sbjct: 204 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 263

Query: 221 IDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFK 280
           I  VR +  QL + V F+HD ++ HTDLKPENIL V++DY   +   NL +  RD    +
Sbjct: 264 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTY---NLEKK-RD----E 323

Query: 281 RVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELC 340
           R  KS+A++V+DFGS T+D +  + IVSTRHYRAPEVIL LGWS PCD+WS+GCI+ E  
Sbjct: 324 RSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYY 383

Query: 341 SGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGRLDWPEGATS----RD 400
            G  LFQTH+N EHLAMMER+LGP+P  M+++  +  +KY  RGRLDW E  ++    R+
Sbjct: 384 VGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRK--QKYFYRGRLDWDENTSAGRYVRE 443

Query: 401 SIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTR 450
           + K +++      L  +  +H   +L  L++ +L Y+P  RLT  EAL+HPFF R
Sbjct: 444 NCKPLRRY-----LTSEAEEHH--QLFDLIESMLEYEPAKRLTLGEALQHPFFAR 481

BLAST of Clc03G03400 vs. ExPASy Swiss-Prot
Match: O35491 (Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2)

HSP 1 Score: 336.7 bits (862), Expect = 4.2e-91
Identity = 170/353 (48.16%), Postives = 239/353 (67.71%), Query Frame = 0

Query: 101 EDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRER-KEMVAIKIVRGIRKYRD 160
           EDD +GH ++ +G+ L  RY+I S +GEGTFG+V++C D  R    VA+KI++ + KY++
Sbjct: 143 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKE 202

Query: 161 AAMIEIEVLQQLGKHDKGG-NRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFP 220
           AA +EI VL+++ + D    N CVQ+ +WFDY  H+CI FE LG S +DFL+ NNY  +P
Sbjct: 203 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 262

Query: 221 IDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFK 280
           I  VR +  QL + V F+HD ++ HTDLKPENIL V++DY   +   NL +  RD    +
Sbjct: 263 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTY---NLEKK-RD----E 322

Query: 281 RVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELC 340
           R  KS+A++V+DFGS T+D +  + IVSTRHYRAPEVIL LGWS PCD+WS+GCI+ E  
Sbjct: 323 RSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYY 382

Query: 341 SGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGRLDWPEGATS----RD 400
            G  LFQTH+N EHLAMMER+LGP+P  M+++  +  +KY  RGRLDW E  ++    R+
Sbjct: 383 VGFTLFQTHDNREHLAMMERILGPVPSRMIRKTRK--QKYFYRGRLDWDENTSAGRYVRE 442

Query: 401 SIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREALRHPFF 448
           + K +++      L  +  DH   +L  L++ +L Y+P  RLT  EAL+HPFF
Sbjct: 443 NCKPLRRY-----LTSEAEDHH--QLFDLIENMLEYEPAKRLTLGEALQHPFF 478

BLAST of Clc03G03400 vs. ExPASy TrEMBL
Match: A0A5A7UP87 (Serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004120 PE=4 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.4e-252
Identity = 426/454 (93.83%), Postives = 430/454 (94.71%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRY
Sbjct: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRE+KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVS DYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD
Sbjct: 241 MIHTDLKPENILLVSGDYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQ+MLK+VDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQDMLKKVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDP HRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPAHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. ExPASy TrEMBL
Match: A0A1S3AXH4 (serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483972 PE=4 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.4e-252
Identity = 426/454 (93.83%), Postives = 430/454 (94.71%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRY
Sbjct: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRE+KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVS DYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD
Sbjct: 241 MIHTDLKPENILLVSGDYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQ+MLK+VDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQDMLKKVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDP HRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPAHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. ExPASy TrEMBL
Match: A0A6J1KKP8 (serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495446 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.5e-251
Identity = 425/454 (93.61%), Postives = 429/454 (94.49%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA K                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIAN+PSFAS+RAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY
Sbjct: 61  CCGQEIANMPSFASSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVSADY+KVHDYKN SRSPRDSSNFKRVPKSS IKVIDFGSTTYDRQD
Sbjct: 241 MIHTDLKPENILLVSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILG+GWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. ExPASy TrEMBL
Match: A0A6J1EDB4 (serine/threonine-protein kinase AFC2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431493 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.5e-251
Identity = 424/454 (93.39%), Postives = 430/454 (94.71%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA K                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIAN+PSFAS+RAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY
Sbjct: 61  CCGQEIANMPSFASSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVSADY+KVHDYKN SRSPRDSSNFKRVPKSS+IKVIDFGSTTYDR+D
Sbjct: 241 MIHTDLKPENILLVSADYMKVHDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDRED 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILG+GWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGEL
Sbjct: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR
Sbjct: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 433

BLAST of Clc03G03400 vs. ExPASy TrEMBL
Match: A0A0A0LTN5 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G048950 PE=4 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.0e-250
Identity = 425/454 (93.61%), Postives = 427/454 (94.05%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK                     AQVGI
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPK---------------------AQVGI 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
           CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY
Sbjct: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KIHSKMGEGTFGQVLECWDRE+KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR
Sbjct: 121 KIHSKMGEGTFGQVLECWDREKKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVS DYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD
Sbjct: 241 MIHTDLKPENILLVSGDYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           QNYIVSTRHYRAPEVILGLGW FPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL
Sbjct: 301 QNYIVSTRHYRAPEVILGLGWRFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GPLPQEMLK+VDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHS GEL
Sbjct: 361 GPLPQEMLKKVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSGGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR 455
           IHLVQGLLRYDP  RLTAREALRHPFFTRD LRR
Sbjct: 421 IHLVQGLLRYDPLLRLTAREALRHPFFTRDSLRR 433

BLAST of Clc03G03400 vs. TAIR 10
Match: AT4G24740.1 (FUS3-complementing gene 2 )

HSP 1 Score: 750.0 bits (1935), Expect = 1.1e-216
Identity = 364/449 (81.07%), Postives = 401/449 (89.31%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKVCVSFTHRGIISDQRSFRSLSAQVGI 60
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A K                     AQVG+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATK---------------------AQVGM 60

Query: 61  CCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY 120
            CGQEI NI SFAS+ APSD+SS+ L VKGVARNGSPPWREDDKDGHY+F LG++LT RY
Sbjct: 61  FCGQEIGNISSFASSGAPSDNSSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRY 120

Query: 121 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 180
           KI+SKMGEGTFGQVLECWDRERKEMVA+KIVRG++KYR+AAMIEIE+LQQLGKHDKGGNR
Sbjct: 121 KIYSKMGEGTFGQVLECWDRERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNR 180

Query: 181 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 240
           CVQIRNWFDYRNHICIVFEKLG SLYDFLRKNNYRSFPIDLVR+IG QLLECVAFMHDLR
Sbjct: 181 CVQIRNWFDYRNHICIVFEKLGSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLR 240

Query: 241 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 300
           MIHTDLKPENILLVS+DYVK+ +YK  SR  RD   +KRVPKSSAIKVIDFGSTTY+RQD
Sbjct: 241 MIHTDLKPENILLVSSDYVKIPEYKG-SRLQRDVC-YKRVPKSSAIKVIDFGSTTYERQD 300

Query: 301 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 360
           Q YIVSTRHYRAPEVILGLGWS+PCD+WSVGCI+VELC+GEALFQTHENLEHLAMMERVL
Sbjct: 301 QTYIVSTRHYRAPEVILGLGWSYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVL 360

Query: 361 GPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGEL 420
           GP PQ+MLK+VDRH+EKYVRRGRLDWP+GATSRDS+KAV KL RL NLIMQHVDHSAGEL
Sbjct: 361 GPFPQQMLKKVDRHSEKYVRRGRLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGEL 420

Query: 421 IHLVQGLLRYDPTHRLTAREALRHPFFTR 450
           I++VQGLLR+DP+ R+TAREALRHPFF R
Sbjct: 421 INMVQGLLRFDPSERITAREALRHPFFAR 425

BLAST of Clc03G03400 vs. TAIR 10
Match: AT3G53570.3 (FUS3-complementing gene 1 )

HSP 1 Score: 637.5 bits (1643), Expect = 8.2e-183
Identity = 311/456 (68.20%), Postives = 359/456 (78.73%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVV------PEAPKVCVSFTHRGIISDQRSFRSL 60
           +E +R +EFPH  +D+RPRKR RL WD        P  P V     + G           
Sbjct: 3   LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYG----------- 62

Query: 61  SAQVGICCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGE 120
                          P FAS   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+
Sbjct: 63  ---------------PEFASGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGD 122

Query: 121 NLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKH 180
            LT RY+I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +H
Sbjct: 123 TLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRH 182

Query: 181 DKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVA 240
           D GG+RCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA
Sbjct: 183 DVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVA 242

Query: 241 FMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGST 300
           +MHDLR+IHTDLKPENILLVS++Y+K+ DYK LSR  +D S FK +PKSSAIK+IDFGST
Sbjct: 243 YMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGST 302

Query: 301 TYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLA 360
           T++ QD NYIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLA
Sbjct: 303 TFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLA 362

Query: 361 MMERVLGPLPQEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSIKAVQKLARLPNLIMQHV 420
           MMERVLGPLP  M+ R DR +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHV
Sbjct: 363 MMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHV 422

Query: 421 DHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTR 450
           DHSAG+LI L+QGLLRYDPT R  AREAL HPFFTR
Sbjct: 423 DHSAGDLIDLLQGLLRYDPTERFKAREALNHPFFTR 431

BLAST of Clc03G03400 vs. TAIR 10
Match: AT3G53570.1 (FUS3-complementing gene 1 )

HSP 1 Score: 637.5 bits (1643), Expect = 8.2e-183
Identity = 311/456 (68.20%), Postives = 359/456 (78.73%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVV------PEAPKVCVSFTHRGIISDQRSFRSL 60
           +E +R +EFPH  +D+RPRKR RL WD        P  P V     + G           
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYG----------- 76

Query: 61  SAQVGICCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGE 120
                          P FAS   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+
Sbjct: 77  ---------------PEFASGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGD 136

Query: 121 NLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKH 180
            LT RY+I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +H
Sbjct: 137 TLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRH 196

Query: 181 DKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVA 240
           D GG+RCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA
Sbjct: 197 DVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVA 256

Query: 241 FMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGST 300
           +MHDLR+IHTDLKPENILLVS++Y+K+ DYK LSR  +D S FK +PKSSAIK+IDFGST
Sbjct: 257 YMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGST 316

Query: 301 TYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLA 360
           T++ QD NYIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLA
Sbjct: 317 TFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLA 376

Query: 361 MMERVLGPLPQEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSIKAVQKLARLPNLIMQHV 420
           MMERVLGPLP  M+ R DR +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHV
Sbjct: 377 MMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHV 436

Query: 421 DHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTR 450
           DHSAG+LI L+QGLLRYDPT R  AREAL HPFFTR
Sbjct: 437 DHSAGDLIDLLQGLLRYDPTERFKAREALNHPFFTR 445

BLAST of Clc03G03400 vs. TAIR 10
Match: AT3G53570.2 (FUS3-complementing gene 1 )

HSP 1 Score: 637.5 bits (1643), Expect = 8.2e-183
Identity = 311/456 (68.20%), Postives = 359/456 (78.73%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVV------PEAPKVCVSFTHRGIISDQRSFRSL 60
           +E +R +EFPH  +D+RPRKR RL WD        P  P V     + G           
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYG----------- 76

Query: 61  SAQVGICCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGE 120
                          P FAS   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+
Sbjct: 77  ---------------PEFASGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGD 136

Query: 121 NLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKH 180
            LT RY+I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +H
Sbjct: 137 TLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRH 196

Query: 181 DKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVA 240
           D GG+RCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA
Sbjct: 197 DVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVA 256

Query: 241 FMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGST 300
           +MHDLR+IHTDLKPENILLVS++Y+K+ DYK LSR  +D S FK +PKSSAIK+IDFGST
Sbjct: 257 YMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGST 316

Query: 301 TYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLA 360
           T++ QD NYIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLA
Sbjct: 317 TFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLA 376

Query: 361 MMERVLGPLPQEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSIKAVQKLARLPNLIMQHV 420
           MMERVLGPLP  M+ R DR +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHV
Sbjct: 377 MMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHV 436

Query: 421 DHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTR 450
           DHSAG+LI L+QGLLRYDPT R  AREAL HPFFTR
Sbjct: 437 DHSAGDLIDLLQGLLRYDPTERFKAREALNHPFFTR 445

BLAST of Clc03G03400 vs. TAIR 10
Match: AT3G53570.4 (FUS3-complementing gene 1 )

HSP 1 Score: 637.5 bits (1643), Expect = 8.2e-183
Identity = 311/456 (68.20%), Postives = 359/456 (78.73%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVV------PEAPKVCVSFTHRGIISDQRSFRSL 60
           +E +R +EFPH  +D+RPRKR RL WD        P  P V     + G           
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYG----------- 76

Query: 61  SAQVGICCGQEIANIPSFASTRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGE 120
                          P FAS   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+
Sbjct: 77  ---------------PEFASGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGD 136

Query: 121 NLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKH 180
            LT RY+I SKMGEGTFGQVLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +H
Sbjct: 137 TLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRH 196

Query: 181 DKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVA 240
           D GG+RCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA
Sbjct: 197 DVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVA 256

Query: 241 FMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGST 300
           +MHDLR+IHTDLKPENILLVS++Y+K+ DYK LSR  +D S FK +PKSSAIK+IDFGST
Sbjct: 257 YMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGST 316

Query: 301 TYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLA 360
           T++ QD NYIVSTRHYRAPEVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLA
Sbjct: 317 TFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLA 376

Query: 361 MMERVLGPLPQEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSIKAVQKLARLPNLIMQHV 420
           MMERVLGPLP  M+ R DR +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHV
Sbjct: 377 MMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHV 436

Query: 421 DHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTR 450
           DHSAG+LI L+QGLLRYDPT R  AREAL HPFFTR
Sbjct: 437 DHSAGDLIDLLQGLLRYDPTERFKAREALNHPFFTR 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894261.12.7e-25294.27serine/threonine-protein kinase AFC2 isoform X1 [Benincasa hispida][more]
XP_008439081.11.3e-25193.83PREDICTED: serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo] >KAA00... [more]
XP_023519374.15.1e-25193.61serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita pepo subsp. pepo... [more]
XP_023001260.11.1e-25093.61serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima][more]
XP_022923920.11.1e-25093.39serine/threonine-protein kinase AFC2 isoform X1 [Cucurbita moschata] >KAG6584116... [more]
Match NameE-valueIdentityDescription
P515671.6e-21581.07Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=... [more]
P515661.2e-18168.20Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=... [more]
P515685.6e-14462.89Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=... [more]
P497603.2e-9147.89Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1[more]
O354914.2e-9148.16Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7UP876.4e-25293.83Serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3AXH46.4e-25293.83serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1KKP85.5e-25193.61serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1EDB45.5e-25193.39serine/threonine-protein kinase AFC2 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0LTN56.0e-25093.61Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G048... [more]
Match NameE-valueIdentityDescription
AT4G24740.11.1e-21681.07FUS3-complementing gene 2 [more]
AT3G53570.38.2e-18368.20FUS3-complementing gene 1 [more]
AT3G53570.18.2e-18368.20FUS3-complementing gene 1 [more]
AT3G53570.28.2e-18368.20FUS3-complementing gene 1 [more]
AT3G53570.48.2e-18368.20FUS3-complementing gene 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 120..447
e-value: 2.3E-75
score: 266.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 120..447
e-value: 1.4E-49
score: 168.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 120..447
score: 37.417656
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 174..447
e-value: 2.8E-112
score: 377.3
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 107..200
e-value: 2.8E-112
score: 377.3
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 114..253
e-value: 3.9E-10
score: 37.0
NoneNo IPR availablePANTHERPTHR45646:SF7SERINE/THREONINE-PROTEIN KINASE AFC2coord: 1..454
NoneNo IPR availablePANTHERPTHR45646SERINE/THREONINE-PROTEIN KINASE DOA-RELATEDcoord: 1..454
NoneNo IPR availableCDDcd14134PKc_CLKcoord: 107..447
e-value: 0.0
score: 562.572
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 241..253
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 107..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc03G03400.2Clc03G03400.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006397 mRNA processing
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity