Clc03G00220 (gene) Watermelon (cordophanus) v2

BLAST of Clc03G00220 vs. NCBI nr
Match: XP_038895283.1 (E3 ubiquitin-protein ligase listerin isoform X4 [Benincasa hispida])

HSP 1 Score: 3464.9 bits (8983), Expect = 0.0e+00
Identity = 1759/1910 (92.09%), Postives = 1820/1910 (95.29%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MG+PKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGS+RLDSSLTGDDA PFS  
Sbjct: 1    MGKPKGDGARSKARPSSSSLAASLLPSDSAANTAGFGGFLGSHRLDSSLTGDDAAPFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE LKQKSGKD AS+IPQWVFEYKKLLMDYNRD
Sbjct: 61   DSEVAQHLKRLSRKD-PTTKLKALASLSEHLKQKSGKDAASVIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDALILCT+EIFMYLEENLKLTPDTLSDK VAKDELEEMHQQVISS+LLALATLIDVLVS
Sbjct: 181  VDALILCTTEIFMYLEENLKLTPDTLSDKAVAKDELEEMHQQVISSTLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
            VR ERSGTGKSSGE KHASKSRS ETAI+FAEKLFTEHKYF+DLLKSKST+VRSATYTVL
Sbjct: 241  VRFERSGTGKSSGETKHASKSRSRETAISFAEKLFTEHKYFIDLLKSKSTIVRSATYTVL 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            RSLVKNIPHAFKEQNMKTIAGSILGAFQE+DPSCHSS+WDTVLLFSKRLPNCWTYVNVQK
Sbjct: 301  RSLVKNIPHAFKEQNMKTIAGSILGAFQEKDPSCHSSMWDTVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ+ISYPALILFLDTVPP AVGGEKFLLDFFENLWVGRNP HSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQQISYPALILFLDTVPPRAVGGEKFLLDFFENLWVGRNPLHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQVTLVDVILVKLLWKDYLH 728
              ERLAFFQ+FKECFL GIRNASS       FCNGDDLAHFQVTLVDVILVKLLWKDYLH
Sbjct: 421  SAERLAFFQSFKECFLRGIRNASS-------FCNGDDLAHFQVTLVDVILVKLLWKDYLH 480

Query: 729  VQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVKHDLLSVF 788
            VQCL+NQDRA S+DAP N+ MAEEIPSIKYPMSYLQDLRKCIVEILSGIHLV HDLLSVF
Sbjct: 481  VQCLKNQDRAVSKDAPFNNKMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVNHDLLSVF 540

Query: 789  SMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTWPLVLLVGPTLANTF 848
            +MEFQK+CISMF LTES+GVASET+E IIGFILELEQLS+DKDDTWPLVLLVGPTLANTF
Sbjct: 541  AMEFQKNCISMFQLTESIGVASETVEQIIGFILELEQLSMDKDDTWPLVLLVGPTLANTF 600

Query: 849  PIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVEAQDVEASQFMQLF 908
            PIIRSLDSSDGVRLLSAAVSVFGPRK+VQ LFIHN+GMSS+QFSGVEAQDVEA QFMQLF
Sbjct: 601  PIIRSLDSSDGVRLLSAAVSVFGPRKVVQELFIHNHGMSSSQFSGVEAQDVEARQFMQLF 660

Query: 909  NEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLESMNSES 968
            NEVFVPWCLQGNNSSASARLDLLL LIDDEHFSDQWHSVISYSTNLDHTEVV+ESMNSES
Sbjct: 661  NEVFVPWCLQGNNSSASARLDLLLALIDDEHFSDQWHSVISYSTNLDHTEVVVESMNSES 720

Query: 969  LAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVAIAQSYAPFRIIISL 1028
            LAVLAKLLDRARVKITNSD RK T TWQKANLG+WH+EHLESAAVAIAQS+APFR   + 
Sbjct: 721  LAVLAKLLDRARVKITNSDTRK-TRTWQKANLGDWHHEHLESAAVAIAQSHAPFRSSFTD 780

Query: 1029 MRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISRPENS 1088
               SVLGGSL NDC SFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSS  ISRP NS
Sbjct: 781  FLCSVLGGSLWNDCSSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSLFISRPGNS 840

Query: 1089 FPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERRQDD 1148
            FPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENY LPSILATIYAIDW+CS+E +QDD
Sbjct: 841  FPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYPLPSILATIYAIDWNCSIEGKQDD 900

Query: 1149 MLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFSEDS 1208
            MLDDK+KEER+A L FGECVRALRQKIT QFWKSCR HDRK+YGSILIQFIRSAIFSEDS
Sbjct: 901  MLDDKYKEERKARLHFGECVRALRQKITKQFWKSCRAHDRKQYGSILIQFIRSAIFSEDS 960

Query: 1209 EEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASNMKN 1268
            EEI SLCCQWMLEILDQISQDHLEEQYMLDQLLIKDD WPFWIAPDFMAPNELAASNMKN
Sbjct: 961  EEIVSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDTWPFWIAPDFMAPNELAASNMKN 1020

Query: 1269 VGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGEN-SSCLGKTTKNEVTSRAWLVAEIL 1328
            VGLDIHKSGNHKLVSLVS+LMSKIG+EKLLSGQ EN SSCLGKTTKNEVTSRAWLVAEIL
Sbjct: 1021 VGLDIHKSGNHKLVSLVSMLMSKIGLEKLLSGQVENSSSCLGKTTKNEVTSRAWLVAEIL 1080

Query: 1329 CTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNIWPY 1388
            CTWKWPGG+ARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYS+R AQSIVNIWPY
Sbjct: 1081 CTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSSRAAQSIVNIWPY 1140

Query: 1389 PVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDCLRILP 1448
            PVSLLEDIQEPFLRAL SFLFSL+KENIWG+DKASSLFEL VSRLFIGEAVNIDCLRILP
Sbjct: 1141 PVSLLEDIQEPFLRALTSFLFSLLKENIWGKDKASSLFELAVSRLFIGEAVNIDCLRILP 1200

Query: 1449 LIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLGQDM 1508
            LIVSYLV PMCETNFTFDDSGSCPGE SLKENIIQNAAEGWLQRVLLFPSLNEWQLGQDM
Sbjct: 1201 LIVSYLVHPMCETNFTFDDSGSCPGEGSLKENIIQNAAEGWLQRVLLFPSLNEWQLGQDM 1260

Query: 1509 EDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHAPSV 1568
            EDWLLLV+SCYPFSSSMGGLQTLK+DRNISTEEGSLLLELFRKQRKTSGRSPAVNHAP V
Sbjct: 1261 EDWLLLVISCYPFSSSMGGLQTLKLDRNISTEEGSLLLELFRKQRKTSGRSPAVNHAPWV 1320

Query: 1569 QMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIVKSSTAM 1628
            QMLLSELMVVSVGYCWK F+DEDWEFLL+QLMSWIQSAVVIMEEIAESVNDIIVKSSTAM
Sbjct: 1321 QMLLSELMVVSVGYCWKLFNDEDWEFLLVQLMSWIQSAVVIMEEIAESVNDIIVKSSTAM 1380

Query: 1629 NLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRLDKLNHL 1688
            NLNEILEKLE+SV ISDPIPFC+SRNALLSFSLF GSLGLQGLKD+ESSSP RLDKLNH+
Sbjct: 1381 NLNEILEKLERSVQISDPIPFCVSRNALLSFSLFNGSLGLQGLKDVESSSPQRLDKLNHV 1440

Query: 1689 NDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDARE 1748
            NDRI E ILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDARE
Sbjct: 1441 NDRIVEGILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDARE 1500

Query: 1749 RALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAIIQENTSCYLD 1808
            RA+KSIEFWGL KG VSSLYGILFS KP+PSLQYAAYVMLSTEPISYSAII ENTSCYLD
Sbjct: 1501 RAVKSIEFWGLCKGAVSSLYGILFSLKPLPSLQYAAYVMLSTEPISYSAIIHENTSCYLD 1560

Query: 1809 YDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQERVNIYLAWSLLLSH 1868
            YDITTEQGSTQVDFSSEYNVLLKEEILL+IEKLPDDVFDMELIAQERVNIYLAWSLLLSH
Sbjct: 1561 YDITTEQGSTQVDFSSEYNVLLKEEILLLIEKLPDDVFDMELIAQERVNIYLAWSLLLSH 1620

Query: 1869 LWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKDTELPAGLSEAATAA 1928
            LWSLPPSSSARERLVQYIQNSASSRILDCLFQHIP+EGMALQKKKDTELPAGLSEAATAA
Sbjct: 1621 LWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTELPAGLSEAATAA 1680

Query: 1929 NQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSSVIE 1988
            NQAITTGSLLFSVEFLWP+EP+KL+ FAGAIFGLM+RVLPAYVRGWFSDLRDRSKS+ IE
Sbjct: 1681 NQAITTGSLLFSVEFLWPIEPVKLSLFAGAIFGLMLRVLPAYVRGWFSDLRDRSKSTAIE 1740

Query: 1989 SFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS 2048
            SFTKAWCSPSLIANEL QIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS
Sbjct: 1741 SFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS 1800

Query: 2049 YPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEEC 2108
            YPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEEC
Sbjct: 1801 YPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEEC 1860

Query: 2109 PICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 2152
            PICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF
Sbjct: 1861 PICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 1901

BLAST of Clc03G00220 vs. NCBI nr
Match: XP_038895280.1 (E3 ubiquitin-protein ligase listerin isoform X2 [Benincasa hispida])

HSP 1 Score: 3464.1 bits (8981), Expect = 0.0e+00
Identity = 1760/1924 (91.48%), Postives = 1822/1924 (94.70%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MG+PKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGS+RLDSSLTGDDA PFS  
Sbjct: 1    MGKPKGDGARSKARPSSSSLAASLLPSDSAANTAGFGGFLGSHRLDSSLTGDDAAPFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE LKQKSGKD AS+IPQWVFEYKKLLMDYNRD
Sbjct: 61   DSEVAQHLKRLSRKD-PTTKLKALASLSEHLKQKSGKDAASVIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDALILCT+EIFMYLEENLKLTPDTLSDK VAKDELEEMHQQVISS+LLALATLIDVLVS
Sbjct: 181  VDALILCTTEIFMYLEENLKLTPDTLSDKAVAKDELEEMHQQVISSTLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
            VR ERSGTGKSSGE KHASKSRS ETAI+FAEKLFTEHKYF+DLLKSKST+VRSATYTVL
Sbjct: 241  VRFERSGTGKSSGETKHASKSRSRETAISFAEKLFTEHKYFIDLLKSKSTIVRSATYTVL 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            RSLVKNIPHAFKEQNMKTIAGSILGAFQE+DPSCHSS+WDTVLLFSKRLPNCWTYVNVQK
Sbjct: 301  RSLVKNIPHAFKEQNMKTIAGSILGAFQEKDPSCHSSMWDTVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ+ISYPALILFLDTVPP AVGGEKFLLDFFENLWVGRNP HSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQQISYPALILFLDTVPPRAVGGEKFLLDFFENLWVGRNPLHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNAS--------------SHFIQNWTFCNGDDLAHFQVTLV 728
              ERLAFFQ+FKECFL GIRNAS               HF    +FCNGDDLAHFQVTLV
Sbjct: 421  SAERLAFFQSFKECFLRGIRNASRWVVVSSPCYFRMGDHFFPCTSFCNGDDLAHFQVTLV 480

Query: 729  DVILVKLLWKDYLHVQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEIL 788
            DVILVKLLWKDYLHVQCL+NQDRA S+DAP N+ MAEEIPSIKYPMSYLQDLRKCIVEIL
Sbjct: 481  DVILVKLLWKDYLHVQCLKNQDRAVSKDAPFNNKMAEEIPSIKYPMSYLQDLRKCIVEIL 540

Query: 789  SGIHLVKHDLLSVFSMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTW 848
            SGIHLV HDLLSVF+MEFQK+CISMF LTES+GVASET+E IIGFILELEQLS+DKDDTW
Sbjct: 541  SGIHLVNHDLLSVFAMEFQKNCISMFQLTESIGVASETVEQIIGFILELEQLSMDKDDTW 600

Query: 849  PLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGV 908
            PLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRK+VQ LFIHN+GMSS+QFSGV
Sbjct: 601  PLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRKVVQELFIHNHGMSSSQFSGV 660

Query: 909  EAQDVEASQFMQLFNEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNL 968
            EAQDVEA QFMQLFNEVFVPWCLQGNNSSASARLDLLL LIDDEHFSDQWHSVISYSTNL
Sbjct: 661  EAQDVEARQFMQLFNEVFVPWCLQGNNSSASARLDLLLALIDDEHFSDQWHSVISYSTNL 720

Query: 969  DHTEVVLESMNSESLAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVA 1028
            DHTEVV+ESMNSESLAVLAKLLDRARVKITNSD RK T TWQKANLG+WH+EHLESAAVA
Sbjct: 721  DHTEVVVESMNSESLAVLAKLLDRARVKITNSDTRK-TRTWQKANLGDWHHEHLESAAVA 780

Query: 1029 IAQSYAPFRIIISLMRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWA 1088
            IAQS+APFR   +    SVLGGSL NDC SFVSRDALIAIFEAVFQKLVSFLLHSPLIWA
Sbjct: 781  IAQSHAPFRSSFTDFLCSVLGGSLWNDCSSFVSRDALIAIFEAVFQKLVSFLLHSPLIWA 840

Query: 1089 RNSSSFLISRPENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIY 1148
            RNSSS  ISRP NSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENY LPSILATIY
Sbjct: 841  RNSSSLFISRPGNSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYPLPSILATIY 900

Query: 1149 AIDWDCSMERRQDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSI 1208
            AIDW+CS+E +QDDMLDDK+KEER+A L FGECVRALRQKIT QFWKSCR HDRK+YGSI
Sbjct: 901  AIDWNCSIEGKQDDMLDDKYKEERKARLHFGECVRALRQKITKQFWKSCRAHDRKQYGSI 960

Query: 1209 LIQFIRSAIFSEDSEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPD 1268
            LIQFIRSAIFSEDSEEI SLCCQWMLEILDQISQDHLEEQYMLDQLLIKDD WPFWIAPD
Sbjct: 961  LIQFIRSAIFSEDSEEIVSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDTWPFWIAPD 1020

Query: 1269 FMAPNELAASNMKNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGEN-SSCLGKTTK 1328
            FMAPNELAASNMKNVGLDIHKSGNHKLVSLVS+LMSKIG+EKLLSGQ EN SSCLGKTTK
Sbjct: 1021 FMAPNELAASNMKNVGLDIHKSGNHKLVSLVSMLMSKIGLEKLLSGQVENSSSCLGKTTK 1080

Query: 1329 NEVTSRAWLVAEILCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLY 1388
            NEVTSRAWLVAEILCTWKWPGG+ARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLY
Sbjct: 1081 NEVTSRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLY 1140

Query: 1389 SNRTAQSIVNIWPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLF 1448
            S+R AQSIVNIWPYPVSLLEDIQEPFLRAL SFLFSL+KENIWG+DKASSLFEL VSRLF
Sbjct: 1141 SSRAAQSIVNIWPYPVSLLEDIQEPFLRALTSFLFSLLKENIWGKDKASSLFELAVSRLF 1200

Query: 1449 IGEAVNIDCLRILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVL 1508
            IGEAVNIDCLRILPLIVSYLV PMCETNFTFDDSGSCPGE SLKENIIQNAAEGWLQRVL
Sbjct: 1201 IGEAVNIDCLRILPLIVSYLVHPMCETNFTFDDSGSCPGEGSLKENIIQNAAEGWLQRVL 1260

Query: 1509 LFPSLNEWQLGQDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRK 1568
            LFPSLNEWQLGQDMEDWLLLV+SCYPFSSSMGGLQTLK+DRNISTEEGSLLLELFRKQRK
Sbjct: 1261 LFPSLNEWQLGQDMEDWLLLVISCYPFSSSMGGLQTLKLDRNISTEEGSLLLELFRKQRK 1320

Query: 1569 TSGRSPAVNHAPSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIA 1628
            TSGRSPAVNHAP VQMLLSELMVVSVGYCWK F+DEDWEFLL+QLMSWIQSAVVIMEEIA
Sbjct: 1321 TSGRSPAVNHAPWVQMLLSELMVVSVGYCWKLFNDEDWEFLLVQLMSWIQSAVVIMEEIA 1380

Query: 1629 ESVNDIIVKSSTAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDM 1688
            ESVNDIIVKSSTAMNLNEILEKLE+SV ISDPIPFC+SRNALLSFSLF GSLGLQGLKD+
Sbjct: 1381 ESVNDIIVKSSTAMNLNEILEKLERSVQISDPIPFCVSRNALLSFSLFNGSLGLQGLKDV 1440

Query: 1689 ESSSPHRLDKLNHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDL 1748
            ESSSP RLDKLNH+NDRI E ILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDL
Sbjct: 1441 ESSSPQRLDKLNHVNDRIVEGILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDL 1500

Query: 1749 IASSVTKSSKDARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPIS 1808
            IASSVTKSSKDARERA+KSIEFWGL KG VSSLYGILFS KP+PSLQYAAYVMLSTEPIS
Sbjct: 1501 IASSVTKSSKDARERAVKSIEFWGLCKGAVSSLYGILFSLKPLPSLQYAAYVMLSTEPIS 1560

Query: 1809 YSAIIQENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQE 1868
            YSAII ENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILL+IEKLPDDVFDMELIAQE
Sbjct: 1561 YSAIIHENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILLLIEKLPDDVFDMELIAQE 1620

Query: 1869 RVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKD 1928
            RVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIP+EGMALQKKKD
Sbjct: 1621 RVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKD 1680

Query: 1929 TELPAGLSEAATAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGW 1988
            TELPAGLSEAATAANQAITTGSLLFSVEFLWP+EP+KL+ FAGAIFGLM+RVLPAYVRGW
Sbjct: 1681 TELPAGLSEAATAANQAITTGSLLFSVEFLWPIEPVKLSLFAGAIFGLMLRVLPAYVRGW 1740

Query: 1989 FSDLRDRSKSSVIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTK 2048
            FSDLRDRSKS+ IESFTKAWCSPSLIANEL QIKKAEFADENFSVVVSKSANEVIATYTK
Sbjct: 1741 FSDLRDRSKSTAIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTK 1800

Query: 2049 DETGMDLVIRLPSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIW 2108
            DETGMDLVIRLPSSYPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIW
Sbjct: 1801 DETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIW 1860

Query: 2109 KRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLC 2152
            KRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLC
Sbjct: 1861 KRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLC 1920

BLAST of Clc03G00220 vs. NCBI nr
Match: XP_038895282.1 (E3 ubiquitin-protein ligase listerin isoform X3 [Benincasa hispida])

HSP 1 Score: 3455.2 bits (8958), Expect = 0.0e+00
Identity = 1759/1924 (91.42%), Postives = 1820/1924 (94.59%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MG+PKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGS+RLDSSLTGDDA PFS  
Sbjct: 1    MGKPKGDGARSKARPSSSSLAASLLPSDSAANTAGFGGFLGSHRLDSSLTGDDAAPFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE LKQKSGKD AS+IPQWVFEYKKLLMDYNRD
Sbjct: 61   DSEVAQHLKRLSRKD-PTTKLKALASLSEHLKQKSGKDAASVIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDALILCT+EIFMYLEENLKLTPDTLSDK VAKDELEEMHQQVISS+LLALATLIDVLVS
Sbjct: 181  VDALILCTTEIFMYLEENLKLTPDTLSDKAVAKDELEEMHQQVISSTLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
            VR ERSGTGKSSGE KHASKSRS ETAI+FAEKLFTEHKYF+DLLKSKST+VRSATYTVL
Sbjct: 241  VRFERSGTGKSSGETKHASKSRSRETAISFAEKLFTEHKYFIDLLKSKSTIVRSATYTVL 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            RSLVKNIPHAFKEQNMKTIAGSILGAFQE+DPSCHSS+WDTVLLFSKRLPNCWTYVNVQK
Sbjct: 301  RSLVKNIPHAFKEQNMKTIAGSILGAFQEKDPSCHSSMWDTVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ+ISYPALILFLDTVPP AVGGEKFLLDFFENLWVGRNP HSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQQISYPALILFLDTVPPRAVGGEKFLLDFFENLWVGRNPLHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQVTLVDVILVKLLWKDYLH 728
              ERLAFFQ+FKECFL GIRNASS       FCNGDDLAHFQVTLVDVILVKLLWKDYLH
Sbjct: 421  SAERLAFFQSFKECFLRGIRNASS-------FCNGDDLAHFQVTLVDVILVKLLWKDYLH 480

Query: 729  VQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVKHDLLSVF 788
            VQCL+NQDRA S+DAP N+ MAEEIPSIKYPMSYLQDLRKCIVEILSGIHLV HDLLSVF
Sbjct: 481  VQCLKNQDRAVSKDAPFNNKMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVNHDLLSVF 540

Query: 789  SMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTWPLVLLVGPTLANTF 848
            +MEFQK+CISMF LTES+GVASET+E IIGFILELEQLS+DKDDTWPLVLLVGPTLANTF
Sbjct: 541  AMEFQKNCISMFQLTESIGVASETVEQIIGFILELEQLSMDKDDTWPLVLLVGPTLANTF 600

Query: 849  PIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVEAQDVEASQFMQLF 908
            PIIRSLDSSDGVRLLSAAVSVFGPRK+VQ LFIHN+GMSS+QFSGVEAQDVEA QFMQLF
Sbjct: 601  PIIRSLDSSDGVRLLSAAVSVFGPRKVVQELFIHNHGMSSSQFSGVEAQDVEARQFMQLF 660

Query: 909  NEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLESMNSES 968
            NEVFVPWCLQGNNSSASARLDLLL LIDDEHFSDQWHSVISYSTNLDHTEVV+ESMNSES
Sbjct: 661  NEVFVPWCLQGNNSSASARLDLLLALIDDEHFSDQWHSVISYSTNLDHTEVVVESMNSES 720

Query: 969  LAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVAIAQSYAPFRIIISL 1028
            LAVLAKLLDRARVKITNSD RK T TWQKANLG+WH+EHLESAAVAIAQS+APFR   + 
Sbjct: 721  LAVLAKLLDRARVKITNSDTRK-TRTWQKANLGDWHHEHLESAAVAIAQSHAPFRSSFTD 780

Query: 1029 MRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISRPENS 1088
               SVLGGSL NDC SFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSS  ISRP NS
Sbjct: 781  FLCSVLGGSLWNDCSSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSLFISRPGNS 840

Query: 1089 FPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERRQDD 1148
            FPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENY LPSILATIYAIDW+CS+E +QDD
Sbjct: 841  FPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYPLPSILATIYAIDWNCSIEGKQDD 900

Query: 1149 MLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFSEDS 1208
            MLDDK+KEER+A L FGECVRALRQKIT QFWKSCR HDRK+YGSILIQFIRSAIFSEDS
Sbjct: 901  MLDDKYKEERKARLHFGECVRALRQKITKQFWKSCRAHDRKQYGSILIQFIRSAIFSEDS 960

Query: 1209 EEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASNMKN 1268
            EEI SLCCQWMLEILDQISQDHLEEQYMLDQLLIKDD WPFWIAPDFMAPNELAASNMKN
Sbjct: 961  EEIVSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDTWPFWIAPDFMAPNELAASNMKN 1020

Query: 1269 VGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGEN-SSCLGKTTKNEVTSRAWLVAEIL 1328
            VGLDIHKSGNHKLVSLVS+LMSKIG+EKLLSGQ EN SSCLGKTTKNEVTSRAWLVAEIL
Sbjct: 1021 VGLDIHKSGNHKLVSLVSMLMSKIGLEKLLSGQVENSSSCLGKTTKNEVTSRAWLVAEIL 1080

Query: 1329 CTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNIWPY 1388
            CTWKWPGG+ARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYS+R AQSIVNIWPY
Sbjct: 1081 CTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSSRAAQSIVNIWPY 1140

Query: 1389 PVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDCLRILP 1448
            PVSLLEDIQEPFLRAL SFLFSL+KENIWG+DKASSLFEL VSRLFIGEAVNIDCLRILP
Sbjct: 1141 PVSLLEDIQEPFLRALTSFLFSLLKENIWGKDKASSLFELAVSRLFIGEAVNIDCLRILP 1200

Query: 1449 LIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLGQDM 1508
            LIVSYLV PMCETNFTFDDSGSCPGE SLKENIIQNAAEGWLQRVLLFPSLNEWQLGQDM
Sbjct: 1201 LIVSYLVHPMCETNFTFDDSGSCPGEGSLKENIIQNAAEGWLQRVLLFPSLNEWQLGQDM 1260

Query: 1509 EDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHAPSV 1568
            EDWLLLV+SCYPFSSSMGGLQTLK+DRNISTEEGSLLLELFRKQRKTSGRSPAVNHAP V
Sbjct: 1261 EDWLLLVISCYPFSSSMGGLQTLKLDRNISTEEGSLLLELFRKQRKTSGRSPAVNHAPWV 1320

Query: 1569 QMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIVKSSTAM 1628
            QMLLSELMVVSVGYCWK F+DEDWEFLL+QLMSWIQSAVVIMEEIAESVNDIIVKSSTAM
Sbjct: 1321 QMLLSELMVVSVGYCWKLFNDEDWEFLLVQLMSWIQSAVVIMEEIAESVNDIIVKSSTAM 1380

Query: 1629 NLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRLDKLNHL 1688
            NLNEILEKLE+SV ISDPIPFC+SRNALLSFSLF GSLGLQGLKD+ESSSP RLDKLNH+
Sbjct: 1381 NLNEILEKLERSVQISDPIPFCVSRNALLSFSLFNGSLGLQGLKDVESSSPQRLDKLNHV 1440

Query: 1689 NDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDARE 1748
            NDRI E ILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDARE
Sbjct: 1441 NDRIVEGILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDARE 1500

Query: 1749 RALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAIIQENTSCYLD 1808
            RA+KSIEFWGL KG VSSLYGILFS KP+PSLQYAAYVMLSTEPISYSAII ENTSCYLD
Sbjct: 1501 RAVKSIEFWGLCKGAVSSLYGILFSLKPLPSLQYAAYVMLSTEPISYSAIIHENTSCYLD 1560

Query: 1809 YDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQERVNIYLAWSLLLSH 1868
            YDITTEQGSTQVDFSSEYNVLLKEEILL+IEKLPDDVFDMELIAQERVNIYLAWSLLLSH
Sbjct: 1561 YDITTEQGSTQVDFSSEYNVLLKEEILLLIEKLPDDVFDMELIAQERVNIYLAWSLLLSH 1620

Query: 1869 LWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKDTELPAGLSEAATAA 1928
            LWSLPPSSSARERLVQYIQNSASSRILDCLFQHIP+EGMALQKKKDTELPAGLSEAATAA
Sbjct: 1621 LWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTELPAGLSEAATAA 1680

Query: 1929 NQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSSVIE 1988
            NQAITTGSLLFSVEFLWP+EP+KL+ FAGAIFGLM+RVLPAYVRGWFSDLRDRSKS+ IE
Sbjct: 1681 NQAITTGSLLFSVEFLWPIEPVKLSLFAGAIFGLMLRVLPAYVRGWFSDLRDRSKSTAIE 1740

Query: 1989 SFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS 2048
            SFTKAWCSPSLIANEL QIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS
Sbjct: 1741 SFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS 1800

Query: 2049 YPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQ--------------NGALAEAIRIW 2108
            YPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQ              NGALAEAIRIW
Sbjct: 1801 YPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQAKAMLCGETNRWSENGALAEAIRIW 1860

Query: 2109 KRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLC 2152
            KRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLC
Sbjct: 1861 KRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLC 1915

BLAST of Clc03G00220 vs. NCBI nr
Match: XP_038895279.1 (E3 ubiquitin-protein ligase listerin isoform X1 [Benincasa hispida])

HSP 1 Score: 3454.5 bits (8956), Expect = 0.0e+00
Identity = 1760/1938 (90.82%), Postives = 1822/1938 (94.01%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MG+PKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGS+RLDSSLTGDDA PFS  
Sbjct: 1    MGKPKGDGARSKARPSSSSLAASLLPSDSAANTAGFGGFLGSHRLDSSLTGDDAAPFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE LKQKSGKD AS+IPQWVFEYKKLLMDYNRD
Sbjct: 61   DSEVAQHLKRLSRKD-PTTKLKALASLSEHLKQKSGKDAASVIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDALILCT+EIFMYLEENLKLTPDTLSDK VAKDELEEMHQQVISS+LLALATLIDVLVS
Sbjct: 181  VDALILCTTEIFMYLEENLKLTPDTLSDKAVAKDELEEMHQQVISSTLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
            VR ERSGTGKSSGE KHASKSRS ETAI+FAEKLFTEHKYF+DLLKSKST+VRSATYTVL
Sbjct: 241  VRFERSGTGKSSGETKHASKSRSRETAISFAEKLFTEHKYFIDLLKSKSTIVRSATYTVL 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            RSLVKNIPHAFKEQNMKTIAGSILGAFQE+DPSCHSS+WDTVLLFSKRLPNCWTYVNVQK
Sbjct: 301  RSLVKNIPHAFKEQNMKTIAGSILGAFQEKDPSCHSSMWDTVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ+ISYPALILFLDTVPP AVGGEKFLLDFFENLWVGRNP HSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQQISYPALILFLDTVPPRAVGGEKFLLDFFENLWVGRNPLHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNAS--------------SHFIQNWTFCNGDDLAHFQVTLV 728
              ERLAFFQ+FKECFL GIRNAS               HF    +FCNGDDLAHFQVTLV
Sbjct: 421  SAERLAFFQSFKECFLRGIRNASRWVVVSSPCYFRMGDHFFPCTSFCNGDDLAHFQVTLV 480

Query: 729  DVILVKLLWKDYLHVQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEIL 788
            DVILVKLLWKDYLHVQCL+NQDRA S+DAP N+ MAEEIPSIKYPMSYLQDLRKCIVEIL
Sbjct: 481  DVILVKLLWKDYLHVQCLKNQDRAVSKDAPFNNKMAEEIPSIKYPMSYLQDLRKCIVEIL 540

Query: 789  SGIHLVKHDLLSVFSMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTW 848
            SGIHLV HDLLSVF+MEFQK+CISMF LTES+GVASET+E IIGFILELEQLS+DKDDTW
Sbjct: 541  SGIHLVNHDLLSVFAMEFQKNCISMFQLTESIGVASETVEQIIGFILELEQLSMDKDDTW 600

Query: 849  PLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGV 908
            PLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRK+VQ LFIHN+GMSS+QFSGV
Sbjct: 601  PLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRKVVQELFIHNHGMSSSQFSGV 660

Query: 909  EAQDVEASQFMQLFNEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNL 968
            EAQDVEA QFMQLFNEVFVPWCLQGNNSSASARLDLLL LIDDEHFSDQWHSVISYSTNL
Sbjct: 661  EAQDVEARQFMQLFNEVFVPWCLQGNNSSASARLDLLLALIDDEHFSDQWHSVISYSTNL 720

Query: 969  DHTEVVLESMNSESLAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVA 1028
            DHTEVV+ESMNSESLAVLAKLLDRARVKITNSD RK T TWQKANLG+WH+EHLESAAVA
Sbjct: 721  DHTEVVVESMNSESLAVLAKLLDRARVKITNSDTRK-TRTWQKANLGDWHHEHLESAAVA 780

Query: 1029 IAQSYAPFRIIISLMRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWA 1088
            IAQS+APFR   +    SVLGGSL NDC SFVSRDALIAIFEAVFQKLVSFLLHSPLIWA
Sbjct: 781  IAQSHAPFRSSFTDFLCSVLGGSLWNDCSSFVSRDALIAIFEAVFQKLVSFLLHSPLIWA 840

Query: 1089 RNSSSFLISRPENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIY 1148
            RNSSS  ISRP NSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENY LPSILATIY
Sbjct: 841  RNSSSLFISRPGNSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYPLPSILATIY 900

Query: 1149 AIDWDCSMERRQDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSI 1208
            AIDW+CS+E +QDDMLDDK+KEER+A L FGECVRALRQKIT QFWKSCR HDRK+YGSI
Sbjct: 901  AIDWNCSIEGKQDDMLDDKYKEERKARLHFGECVRALRQKITKQFWKSCRAHDRKQYGSI 960

Query: 1209 LIQFIRSAIFSEDSEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPD 1268
            LIQFIRSAIFSEDSEEI SLCCQWMLEILDQISQDHLEEQYMLDQLLIKDD WPFWIAPD
Sbjct: 961  LIQFIRSAIFSEDSEEIVSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDTWPFWIAPD 1020

Query: 1269 FMAPNELAASNMKNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGEN-SSCLGKTTK 1328
            FMAPNELAASNMKNVGLDIHKSGNHKLVSLVS+LMSKIG+EKLLSGQ EN SSCLGKTTK
Sbjct: 1021 FMAPNELAASNMKNVGLDIHKSGNHKLVSLVSMLMSKIGLEKLLSGQVENSSSCLGKTTK 1080

Query: 1329 NEVTSRAWLVAEILCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLY 1388
            NEVTSRAWLVAEILCTWKWPGG+ARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLY
Sbjct: 1081 NEVTSRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLY 1140

Query: 1389 SNRTAQSIVNIWPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLF 1448
            S+R AQSIVNIWPYPVSLLEDIQEPFLRAL SFLFSL+KENIWG+DKASSLFEL VSRLF
Sbjct: 1141 SSRAAQSIVNIWPYPVSLLEDIQEPFLRALTSFLFSLLKENIWGKDKASSLFELAVSRLF 1200

Query: 1449 IGEAVNIDCLRILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVL 1508
            IGEAVNIDCLRILPLIVSYLV PMCETNFTFDDSGSCPGE SLKENIIQNAAEGWLQRVL
Sbjct: 1201 IGEAVNIDCLRILPLIVSYLVHPMCETNFTFDDSGSCPGEGSLKENIIQNAAEGWLQRVL 1260

Query: 1509 LFPSLNEWQLGQDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRK 1568
            LFPSLNEWQLGQDMEDWLLLV+SCYPFSSSMGGLQTLK+DRNISTEEGSLLLELFRKQRK
Sbjct: 1261 LFPSLNEWQLGQDMEDWLLLVISCYPFSSSMGGLQTLKLDRNISTEEGSLLLELFRKQRK 1320

Query: 1569 TSGRSPAVNHAPSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIA 1628
            TSGRSPAVNHAP VQMLLSELMVVSVGYCWK F+DEDWEFLL+QLMSWIQSAVVIMEEIA
Sbjct: 1321 TSGRSPAVNHAPWVQMLLSELMVVSVGYCWKLFNDEDWEFLLVQLMSWIQSAVVIMEEIA 1380

Query: 1629 ESVNDIIVKSSTAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDM 1688
            ESVNDIIVKSSTAMNLNEILEKLE+SV ISDPIPFC+SRNALLSFSLF GSLGLQGLKD+
Sbjct: 1381 ESVNDIIVKSSTAMNLNEILEKLERSVQISDPIPFCVSRNALLSFSLFNGSLGLQGLKDV 1440

Query: 1689 ESSSPHRLDKLNHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDL 1748
            ESSSP RLDKLNH+NDRI E ILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDL
Sbjct: 1441 ESSSPQRLDKLNHVNDRIVEGILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDL 1500

Query: 1749 IASSVTKSSKDARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPIS 1808
            IASSVTKSSKDARERA+KSIEFWGL KG VSSLYGILFS KP+PSLQYAAYVMLSTEPIS
Sbjct: 1501 IASSVTKSSKDARERAVKSIEFWGLCKGAVSSLYGILFSLKPLPSLQYAAYVMLSTEPIS 1560

Query: 1809 YSAIIQENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQE 1868
            YSAII ENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILL+IEKLPDDVFDMELIAQE
Sbjct: 1561 YSAIIHENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILLLIEKLPDDVFDMELIAQE 1620

Query: 1869 RVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKD 1928
            RVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIP+EGMALQKKKD
Sbjct: 1621 RVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKD 1680

Query: 1929 TELPAGLSEAATAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGW 1988
            TELPAGLSEAATAANQAITTGSLLFSVEFLWP+EP+KL+ FAGAIFGLM+RVLPAYVRGW
Sbjct: 1681 TELPAGLSEAATAANQAITTGSLLFSVEFLWPIEPVKLSLFAGAIFGLMLRVLPAYVRGW 1740

Query: 1989 FSDLRDRSKSSVIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTK 2048
            FSDLRDRSKS+ IESFTKAWCSPSLIANEL QIKKAEFADENFSVVVSKSANEVIATYTK
Sbjct: 1741 FSDLRDRSKSTAIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTK 1800

Query: 2049 DETGMDLVIRLPSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQ----------- 2108
            DETGMDLVIRLPSSYPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQ           
Sbjct: 1801 DETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQAKAMLCGETNR 1860

Query: 2109 ---NGALAEAIRIWKRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLY 2152
               NGALAEAIRIWKRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLY
Sbjct: 1861 WSENGALAEAIRIWKRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLY 1920

BLAST of Clc03G00220 vs. NCBI nr
Match: XP_008457305.1 (PREDICTED: E3 ubiquitin-protein ligase listerin isoform X1 [Cucumis melo])

HSP 1 Score: 3332.0 bits (8638), Expect = 0.0e+00
Identity = 1691/1913 (88.40%), Postives = 1777/1913 (92.89%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MGRPKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGSYRLDSSLTGDDA PFS  
Sbjct: 1    MGRPKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSSLTGDDAAPFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE+LKQKSGKDVASIIPQWVFEYKKLLMDYNRD
Sbjct: 61   DSEVAQHLKRLSRKD-PTTKLKALASLSEILKQKSGKDVASIIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDAL+LCT+EIF+YLEENLKLTPDTLSDK+VAKDELEEMHQQVISSSLLALATLIDVLVS
Sbjct: 181  VDALVLCTTEIFIYLEENLKLTPDTLSDKLVAKDELEEMHQQVISSSLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
             RSERSGTGKSSGE KH S SRS ETAI+FAEKLFTEHKYF+DLLKSKS +VRSATY+V+
Sbjct: 241  GRSERSGTGKSSGETKHTSMSRSRETAISFAEKLFTEHKYFIDLLKSKSNIVRSATYSVM 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            RSLVKNIPHAFKEQNMKTIAGSILGAFQE+DPSCHS +WD VLLFSKRLPNCWTYVNVQK
Sbjct: 301  RSLVKNIPHAFKEQNMKTIAGSILGAFQEKDPSCHSPMWDAVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ+ISYP LILFLDTVPP AVGGEKFLLDFFENLWVGRNPFHSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQKISYPTLILFLDTVPPCAVGGEKFLLDFFENLWVGRNPFHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQVTLVDVILVKLLWKDYLH 728
              ERLAFFQAFKECFLWGI+NASS       FCNGDD  HFQVTL+D ILVKLLWKDYLH
Sbjct: 421  STERLAFFQAFKECFLWGIQNASS-------FCNGDDFVHFQVTLIDAILVKLLWKDYLH 480

Query: 729  VQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVKHDLLSVF 788
            VQCL+NQDR FSEDAPLN+ M E++PS KYPMSYLQDLRKCIVEILS IHL KHDLLSVF
Sbjct: 481  VQCLKNQDRVFSEDAPLNNKMTEDLPSTKYPMSYLQDLRKCIVEILSSIHLAKHDLLSVF 540

Query: 789  SMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTWPLVLLVGPTLANTF 848
            +MEFQK+CI MF LT+++GVASETIE IIGFILELEQLS+DKDDTW LV LVGPTLANTF
Sbjct: 541  AMEFQKNCIDMFQLTDNVGVASETIEQIIGFILELEQLSMDKDDTWLLVHLVGPTLANTF 600

Query: 849  PIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVEAQDVEASQFMQLF 908
            PII+SLDS DGVRLLSAAVSVFGPRKIV+ LFI+NNGMSST+FSGVEAQD+EA QFMQ+F
Sbjct: 601  PIIQSLDSLDGVRLLSAAVSVFGPRKIVRELFINNNGMSSTKFSGVEAQDLEARQFMQVF 660

Query: 909  NEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLESMNSES 968
            N++FVPWCLQGNNSS+SA+LDLLL LIDDEHFSDQWHSVISYSTNL+HTEVVLESMNSES
Sbjct: 661  NDIFVPWCLQGNNSSSSAQLDLLLALIDDEHFSDQWHSVISYSTNLNHTEVVLESMNSES 720

Query: 969  LAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVAIAQSYAPFRIIISL 1028
            LAVLAKLL+R R KITNSD RK+THTWQ+ANLGNWH+EHLESAA+AIAQS+AP R   + 
Sbjct: 721  LAVLAKLLNRVRGKITNSDARKVTHTWQRANLGNWHHEHLESAAIAIAQSHAPIRSSFTD 780

Query: 1029 MRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISR---P 1088
               SVLGGS+QNDC SFVSRDALIAIFEA+FQKLVSFLLHSP  WARNS S LISR   P
Sbjct: 781  FLCSVLGGSVQNDCSSFVSRDALIAIFEALFQKLVSFLLHSPFTWARNSCSLLISRPDSP 840

Query: 1089 ENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERR 1148
            E  FPK T SSEVV MA+FALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSME +
Sbjct: 841  EKIFPKYT-SSEVVVMANFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMEGK 900

Query: 1149 QDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFS 1208
            QDD+LD+KFKEE +A LLFGE VRALRQKITDQFWKSCRTH+RKKYGSILIQFIRSAIFS
Sbjct: 901  QDDVLDEKFKEESKARLLFGESVRALRQKITDQFWKSCRTHNRKKYGSILIQFIRSAIFS 960

Query: 1209 EDSEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASN 1268
            EDSEEI SLC QWMLEILD ISQD  EEQYMLDQLLIK+D WPFWIAPDFMAPNE AASN
Sbjct: 961  EDSEEIVSLCLQWMLEILDHISQDQFEEQYMLDQLLIKNDTWPFWIAPDFMAPNEFAASN 1020

Query: 1269 MKNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSS-CLGKTTKNEVTSRAWLVA 1328
             KN+GLDIH SGNHK +SL+S+ MSKIG+EKL SGQ ENSS C+ K TKNEVTSRAWLVA
Sbjct: 1021 TKNIGLDIHISGNHKFISLISMFMSKIGLEKLFSGQVENSSPCISKMTKNEVTSRAWLVA 1080

Query: 1329 EILCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNI 1388
            EILCTWKWPGG+ARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYS+R AQS++NI
Sbjct: 1081 EILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSSRAAQSLINI 1140

Query: 1389 WPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDCLR 1448
            WPYPVSLLEDIQEPFLRALAS LFSL+KENIWGRDKASS FELLVSRLFIGEAVNIDCLR
Sbjct: 1141 WPYPVSLLEDIQEPFLRALASLLFSLLKENIWGRDKASSQFELLVSRLFIGEAVNIDCLR 1200

Query: 1449 ILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLG 1508
            ILPLI+SYLVRPMCE N TFDD GSC G+ SL EN  Q   EGWLQRVLLFPSLNEWQLG
Sbjct: 1201 ILPLILSYLVRPMCERNSTFDDCGSCSGD-SLMENTFQRTTEGWLQRVLLFPSLNEWQLG 1260

Query: 1509 QDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHA 1568
            QDME WLLLV+SCYPFS S+GGLQTLK+DRNISTEEGSLLLELFRKQRK S RSPAVNHA
Sbjct: 1261 QDMEYWLLLVISCYPFSCSIGGLQTLKLDRNISTEEGSLLLELFRKQRKASSRSPAVNHA 1320

Query: 1569 PSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIVKSS 1628
            P VQMLLSELMVVSVGYCWKQF  EDWEFLL QLMSWIQSAVVIMEEIAESVNDIIVKSS
Sbjct: 1321 PWVQMLLSELMVVSVGYCWKQFSHEDWEFLLFQLMSWIQSAVVIMEEIAESVNDIIVKSS 1380

Query: 1629 TAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRLDKL 1688
            TAM+LNEILEKLEQSVLI DPIPFCISRNALLSFSLF GSLGL GLKDMESSSP + DKL
Sbjct: 1381 TAMDLNEILEKLEQSVLILDPIPFCISRNALLSFSLFDGSLGLHGLKDMESSSPQQFDKL 1440

Query: 1689 NHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKD 1748
            NH+NDRI E ILRMFFCTGISEAIA S SDKAASIISSSRLELPYFWDLIASSVTKSSKD
Sbjct: 1441 NHVNDRIVEGILRMFFCTGISEAIAYSFSDKAASIISSSRLELPYFWDLIASSVTKSSKD 1500

Query: 1749 ARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAIIQENTSC 1808
            ARERA+KSIEFWGLSKGPVSSLYGILFS KP+PSLQYAAYVMLSTEPIS SAII+ENTSC
Sbjct: 1501 ARERAVKSIEFWGLSKGPVSSLYGILFSPKPIPSLQYAAYVMLSTEPISNSAIIRENTSC 1560

Query: 1809 YLDYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQERVNIYLAWSLL 1868
            YLDYDITTEQ STQVDFSSEYNVLLKEEIL MIEKLPDDVF+MELIAQERVNIYLAWSLL
Sbjct: 1561 YLDYDITTEQRSTQVDFSSEYNVLLKEEILCMIEKLPDDVFEMELIAQERVNIYLAWSLL 1620

Query: 1869 LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKDTELPAGLSEAA 1928
            LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIP+EGMALQK+KDTELPAGLSEAA
Sbjct: 1621 LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKRKDTELPAGLSEAA 1680

Query: 1929 TAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSS 1988
            TAANQAITTGSLLFSVEFLWP+EP+KLASFAGAIFGLM+RVLPAYVRGWFSDLRDRSKSS
Sbjct: 1681 TAANQAITTGSLLFSVEFLWPIEPVKLASFAGAIFGLMLRVLPAYVRGWFSDLRDRSKSS 1740

Query: 1989 VIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL 2048
            V+ESFTKAWCSPS+IANEL QIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL
Sbjct: 1741 VLESFTKAWCSPSIIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL 1800

Query: 2049 PSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV 2108
            PSSYPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV
Sbjct: 1801 PSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV 1860

Query: 2109 EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 2152
            EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF
Sbjct: 1861 EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 1903

BLAST of Clc03G00220 vs. ExPASy Swiss-Prot
Match: Q9FGI1 (E3 ubiquitin-protein ligase listerin OS=Arabidopsis thaliana OX=3702 GN=At5g58410 PE=3 SV=1)

HSP 1 Score: 1652.1 bits (4277), Expect = 0.0e+00
Identity = 912/1930 (47.25%), Postives = 1271/1930 (65.85%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFSVW 308
            MG+PKGD ARSKARPSSSSLAASLLPS SAA V GFGG++GS R  +SL+ +D+  F + 
Sbjct: 1    MGKPKGDAARSKARPSSSSLAASLLPSGSAAAV-GFGGYVGSSRFQTSLSNEDSASF-LD 60

Query: 309  LFKRVATSHYYFQ------QLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
            L   VA             ++KALASLSEL+KQK GK++  IIPQW FEYKKL++DY+RD
Sbjct: 61   LDSEVAQHLQRLSRKDPTTKIKALASLSELVKQKQGKELLPIIPQWTFEYKKLILDYSRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQ--------- 428
            VRRATHD MTN+V  AGR+IAPHLKS+MGPWWFSQFD  SEVSQ+A  S Q         
Sbjct: 121  VRRATHDVMTNVVTGAGRDIAPHLKSIMGPWWFSQFDLASEVSQAAKSSFQVGSSFGNSV 180

Query: 429  ----AAFPAQEKRVDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSS 488
                AAFPAQEKR+ AL LC++EIF YLEENLKLTP  LSDK +A DELEEM+QQ+ISSS
Sbjct: 181  FLVEAAFPAQEKRLHALNLCSAEIFAYLEENLKLTPQNLSDKSLASDELEEMYQQMISSS 240

Query: 489  LLALATLIDVLVSVRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKS 548
            L+ LATL+D+L+    + +G+   + E K ASK+R+   A + AEK+F+ HK F++ LKS
Sbjct: 241  LVGLATLLDILLR-EPDNTGSANINSESKLASKARA--VATSSAEKMFSSHKCFLNFLKS 300

Query: 549  KSTMVRSATYTVLRSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSK 608
            +S  +RSATY++L S +KN+P  F E +++++A ++LG F+E +P+CHSS+W+ VLLFSK
Sbjct: 301  ESPSIRSATYSLLSSFIKNVPEVFGEGDVRSLAPALLGVFRENNPTCHSSMWEAVLLFSK 360

Query: 609  RLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFF 668
            + P  W Y+NV K+VLN  W FLRNGC+GS ++SYPALILFL+ +P  +V  +KF ++FF
Sbjct: 361  KFPQSWVYLNVHKSVLNHLWQFLRNGCYGSPQVSYPALILFLEVMPAQSVESDKFFVNFF 420

Query: 669  ENLWVGRNPFHSSRVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAH-FQVTLV 728
            +NL  GR+   SS  ++L+  +A  ECFLWG+RNAS        +C+  +  H  QV L+
Sbjct: 421  KNLLAGRSMCESSSTDQLSLLRATTECFLWGLRNAS-------RYCDVPNSIHDLQVDLI 480

Query: 729  DVILVKLLWKDYLHVQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEIL 788
            D +LVK+LW D+  +              P N   + E   +   +SYLQ+L +CI+EIL
Sbjct: 481  DKVLVKILWADFTELS---------KGSIPPNQRKSAENLGMGNSVSYLQELGRCILEIL 540

Query: 789  SGIHLVKHDLLSVFSMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTW 848
            SGI+L++ +LLS F    Q+  ++M    + + + + ++  +I F+L LE+ SV + ++W
Sbjct: 541  SGINLLEQNLLSFFCKAVQESFLNMLQQGD-LEIVAGSMRKMIDFLLLLERYSVLEGESW 600

Query: 849  PLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGV 908
            PL   +GP L+  FP IRS +  DGV+LLS +VSVFGPRK+V  L   ++  +ST  S  
Sbjct: 601  PLHQFMGPLLSKAFPWIRSSELLDGVKLLSVSVSVFGPRKVVPVLI--DDIETSTLLSVE 660

Query: 909  EAQDVEASQFMQLFNEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNL 968
            + +++   + +++F E+F+PWC+ G +SS +AR DLL  L+DDE F+ QW  VISY  N 
Sbjct: 661  KEKNMSPEKLIKVFQEIFIPWCMDGYDSSTAARQDLLFSLLDDECFTQQWSDVISYVFNQ 720

Query: 969  DHTEVVLESMNSESLAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVA 1028
             H           +LA +  LL++AR +IT     +  +    +   +WH+  +ES A++
Sbjct: 721  QH-------QGFNNLAAMKMLLEKARDEITKRSSGQELNQRIGSRPEHWHHTLIESTAIS 780

Query: 1029 IAQSYAPFRIIISLMRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWA 1088
            +  S +           SVLGGS Q+   SFVSR +L+ I+  + +KL+SF+  SPL   
Sbjct: 781  LVHSSSATTTSAVQFLCSVLGGSTQDSSISFVSRSSLVLIYRGILEKLLSFIKQSPLCSV 840

Query: 1089 RNSSSFLISRPENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIY 1148
             ++ S LI   E     S+SS +V+ +A FA EV+D   F L +L ++  LL ++L++I+
Sbjct: 841  NDTCSSLI--VEAIAFDSSSSVDVIVVAKFAAEVIDGSFFSLKSLSQDATLLTTVLSSIF 900

Query: 1149 AIDWDCSMERRQDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSI 1208
             ID +  M    D+ L +  KE+R+      + V A+  K+ +QFWKS     RK   S 
Sbjct: 901  IIDLENRMTSLVDNTLSES-KEKRKDRNFVCDYVHAVCSKMDNQFWKSINYDVRKSSAST 960

Query: 1209 LIQFIRSAIFSEDS---EEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWI 1268
            L QF+RS +  ED     E+  LC   M E+L+ +S D  +E+ +   LL++ D WP W+
Sbjct: 961  LAQFLRSVVLLEDDLQPFELTLLCASRMTEVLEYLSLDQSDEENICGLLLLESDAWPIWV 1020

Query: 1269 APDFMAPNELAASNMKNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSSCLGKT 1328
            +P   A   +    M     ++ KS + + VS +  L+ K+GI + + G  ++       
Sbjct: 1021 SPSSSA--SIDTHGMPVQLCELRKSKSQRYVSFIDSLIMKLGIHRFIVGHKDHG------ 1080

Query: 1329 TKNEVTSRAWLVAEILCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGAL 1388
                  S+AWL  EILCTW+WPGG  + SFLP   ++ K   S   LL+S F++LL+GAL
Sbjct: 1081 ----FASQAWLSVEILCTWEWPGGKVQTSFLPNLVSFCKDEPSSGGLLNSIFDILLNGAL 1140

Query: 1389 LYSNRTAQSIVNIWPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSR 1448
            ++     + + N+W    + + D+ EPFLRAL SFL  L KE++WG ++A + F+++  +
Sbjct: 1141 VHVKDEEEGLGNMWVDFNNNIVDVVEPFLRALVSFLHILFKEDLWGEEEAMAAFKMITDK 1200

Query: 1449 LFIGEAVNIDCLRILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQR 1508
            LFIGE  + +CLRI+P I+S ++ P+     T   SG    +  L   ++      WL+R
Sbjct: 1201 LFIGEETSKNCLRIIPYIMSIIISPL----RTKVKSGGSGKDTLLPLEVL---LRNWLER 1260

Query: 1509 VLLFPSLNEWQLGQDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQ 1568
             L FP L  WQ G+D++DW  LV+SCYP S      +  ++ R++STEE +LLL+LFRKQ
Sbjct: 1261 SLSFPPLVLWQSGEDIQDWFQLVISCYPVSDK--AEEAKELQRHLSTEERTLLLDLFRKQ 1320

Query: 1569 RKTSGRSPAVNHAPSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEE 1628
            ++  G S  V   P+VQ+LL+ L++++V YC   F+++DW+F+   L   IQSAVV+MEE
Sbjct: 1321 KQDPGASTVVTQLPAVQILLARLIMIAVSYCGNDFNEDDWDFVFSNLKRLIQSAVVVMEE 1380

Query: 1629 IAESVNDII--VKSSTAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQG 1688
             +E+VND I  V S      N+ LE L   V ISDP     ++NAL +FSL    +  + 
Sbjct: 1381 TSENVNDFISGVSSMEKEKENDTLEGLGHIVFISDP-SINSAQNALSAFSLLNALVNHKS 1440

Query: 1689 LKDMESSSPHRLDKLNHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPY 1748
            ++  ++      +  + + DRI E +LR+FFCTG++EAIA S S +AASI++S R++   
Sbjct: 1441 VEGEDNLKSLADETWDPVKDRILEGVLRLFFCTGLTEAIAASYSPEAASIVASFRVDHLQ 1500

Query: 1749 FWDLIASSVTKSSKDARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLST 1808
            FW+L+A  V  SS  AR+RA++++EFWGLS+G +SSLY I+FS  P+PSLQ AAY +LST
Sbjct: 1501 FWELVAHLVVDSSPRARDRAVRAVEFWGLSRGSISSLYAIMFSSNPIPSLQLAAYTVLST 1560

Query: 1809 EPISYSAIIQENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMEL 1868
            EPIS  AI+ +  +  L+ +   +Q S+     SE  +LL++E+  M+EKL  ++ D +L
Sbjct: 1561 EPISRLAIVAD-LNAPLNDESLNDQDSSNAGLPSEDKLLLRDEVSCMVEKLDHELLDTDL 1620

Query: 1869 IAQERVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIE---GM 1928
             A ERV  +LAWSLLLS++ SLP  +  RERLVQYI+ +A+  ILD LFQHIP+E   G 
Sbjct: 1621 TAPERVQTFLAWSLLLSNVNSLPSLTQGRERLVQYIEKTANPLILDSLFQHIPLELYMGQ 1680

Query: 1929 ALQKKKDTELPAGLSEAATAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVL 1988
            +L KKKD ++P+ LS  A+AA +AI TGS L +VE LWP+E  K+AS AGAI+GLM+RVL
Sbjct: 1681 SL-KKKDGDIPSELSVVASAATRAIITGSSLSTVESLWPIETGKMASLAGAIYGLMLRVL 1740

Query: 1989 PAYVRGWFSDLRDRSKSSVIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANE 2048
            PAYVR WFS++RDRS SS+IE+FT+ WCSPSLI NEL QIKKA+F DE+FSV +SK+ANE
Sbjct: 1741 PAYVREWFSEMRDRSASSLIEAFTRTWCSPSLIKNELSQIKKADFNDESFSVSISKAANE 1800

Query: 2049 VIATYTKDETGMDLVIRLPSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGAL 2108
            V+ATYTKDETGMDLVIRLP SYPL+ VDV C +S+GISE KQRKWL+SM  FVR+QNGAL
Sbjct: 1801 VVATYTKDETGMDLVIRLPVSYPLKPVDVNCAKSIGISEAKQRKWLMSMQMFVRHQNGAL 1860

Query: 2109 AEAIRIWKRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSH 2151
            AEAIRIWKRN DKEFEGVE+CPICYSVIH  NHS+PR AC TCK+KFH ACL KWF TS+
Sbjct: 1861 AEAIRIWKRNSDKEFEGVEDCPICYSVIHIGNHSLPRRACVTCKYKFHKACLDKWFYTSN 1872

BLAST of Clc03G00220 vs. ExPASy Swiss-Prot
Match: O94822 (E3 ubiquitin-protein ligase listerin OS=Homo sapiens OX=9606 GN=LTN1 PE=1 SV=6)

HSP 1 Score: 275.0 bits (702), Expect = 7.1e-72
Identity = 439/2008 (21.86%), Postives = 799/2008 (39.79%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGF-------------LGSYRLDS 308
            MG       +   RPS+S  AA LL  +    V GF GF              G+  +DS
Sbjct: 1    MGGKNKQRTKGNLRPSNSGRAAELLAKEQ-GTVPGFIGFGTSQSDLGYVPAIQGAEEIDS 60

Query: 309  SLTGDDATPFSVWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKL 368
             +  D          K V T      +LKA+     +  ++  + V  ++P W   + K+
Sbjct: 61   LVDSDFRMVLRKLSKKDVTT------KLKAMQEFGTMCTERDTETVKGVLPYWPRIFCKI 120

Query: 369  LMDYNRDVRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAA 428
             +D++R VR AT      L++   +++AP+LKSLMG W  +Q D+ +  + +A  + +AA
Sbjct: 121  SLDHDRRVREATQQAFEKLILKVKKQLAPYLKSLMGYWLMAQCDTYTPAAFAAKDAFEAA 180

Query: 429  FPAQEKRVDALILCTSEIFMYLEENL-KLTPDTLSD-KVVAKDELEEMHQQVISSSLLAL 488
            FP   K+ +A+  C  EI   L+++L K TPDTLSD + V ++E E    +V++ SLLAL
Sbjct: 181  FP-PSKQPEAIAFCKDEITSVLQDHLIKETPDTLSDPQTVPEEEREAKFYRVVTCSLLAL 240

Query: 489  ATLIDVLVSVRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTM 548
              L+ +L                ++   KS            L +++K++    K     
Sbjct: 241  KRLLCLL---------PDNELDSLEEKFKS------------LLSQNKFW-KYGKHSVPQ 300

Query: 549  VRSATYTVLRSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPN 608
            +RSA + ++ +L + IP   KE+  K ++ S+L +  + DP    ++W+ VL     + +
Sbjct: 301  IRSAYFELVSALCQRIPQLMKEEASK-VSPSVLLSIDDSDPIVCPALWEAVLYTLTTIED 360

Query: 609  CWTYVNVQKTVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENL- 668
            CW +VN +K+V  +    +R G  G   + YP L+ F+  +P      +   LDFF+N  
Sbjct: 361  CWLHVNAKKSVFPKLSTVIREGGRGLATVIYPYLLPFISKLPQSITNPK---LDFFKNFL 420

Query: 669  -----WVGRNPFHSSRVERLAFFQAFKECFLWGI-RNASSHFIQNWTFCNGDDLAHFQVT 728
                  +      +S +E  A   AF EC  + + +N     I+       D L  F   
Sbjct: 421  TSLVAGLSTERTKTSSLESSAVISAFFECLRFIMQQNLGEEEIEQMLV--NDQLIPF--- 480

Query: 729  LVDVILVKLLWKD--YLHVQCLENQDRAFSE-----DAPLNDNMAEEIPSIKYPMSYLQD 788
             +D +L     KD    H Q   +     S      D   ++  A  + ++   + + + 
Sbjct: 481  -IDAVL-----KDPGLQHGQLFNHLAETLSSWEAKADTEKDEKTAHNLENVL--IHFWER 540

Query: 789  LRKCIVEILSGIHLVKHDLLSVFSM--EFQKDCISMFHLTESMG---------------- 848
            L +  V  +S        +L V ++    QK   S+    +  G                
Sbjct: 541  LSEICVAKISEPEADVESVLGVSNLLQVLQKPKSSLKSSKKKNGKVRFADEILESNKENE 600

Query: 849  --VASETIELIIGFILELEQLSVDKDD-------TWPLVLLVGPTLANTFPIIRSLDSSD 908
              V+SE  E I G+ L  E                 PL  LV      +   +    S  
Sbjct: 601  KCVSSEG-EKIEGWELTTEPSLTHNSSGLLSPLRKKPLEDLVCKLADISINYVNERKSEQ 660

Query: 909  GVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVE-AQDVEASQFMQLFNEVFVPWCL 968
             +R LS  +  F   ++ + L + +   S  Q   +E A+ V+ +  +Q   +  + W  
Sbjct: 661  HLRFLSTLLDSFSSSRVFKML-LGDEKQSIVQAKPLEIAKLVQKNPAVQFLYQKLIGWLN 720

Query: 969  QGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLESMNSESLA--VLAKL 1028
            +         +D+L             +S +    N    + VL+ +    L    L K+
Sbjct: 721  EDQRKDFGFLVDIL-------------YSALRCCDNDMERKKVLDDLTKVDLKWNSLLKI 780

Query: 1029 LDRARVKITNSDVRKLTHTWQK--------ANLGN-WHNEHLESAAVAIAQSYAPFRIII 1088
            +++A     +SD   L   W K         NL +   NE LES  V+    ++    ++
Sbjct: 781  IEKA---CPSSDKHALVTPWLKGDILGEKLVNLADCLCNEDLES-RVSSESHFSERWTLL 840

Query: 1089 SLMRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISRPE 1148
            SL+ S              V  D LI     V+ + +   LH  L   +  S        
Sbjct: 841  SLVLS------------QHVKNDYLIG---DVYVERIIVRLHETLFKTKKLSE------- 900

Query: 1149 NSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERRQ 1208
                  +S S V  +   A         CL     E+ LL     T++ +      +   
Sbjct: 901  ----AESSDSSVSFICDVAYNYFSSAKGCLLMPSSEDLLL-----TLFQLCAQSKEKTHL 960

Query: 1209 DDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFSE 1268
             D L  K K    +G      V  L  +    + +S   H              SA++ +
Sbjct: 961  PDFLICKLKNTWLSG------VNLLVHQTDSSYKESTFLH-------------LSALWLK 1020

Query: 1269 DSEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASNM 1328
            +  +  SL    +  +L  +         +L+ LL  +D +   +    + PN+     M
Sbjct: 1021 NQVQASSLDINSLQVLLSAVDD-------LLNTLLESEDSYLMGVYIGSVMPNDSEWEKM 1080

Query: 1329 K---------NVGLDIHKSGNHKLVSL------VSLLMSKIGIEKLLSGQGENSSCLGKT 1388
            +            L+   S N++          +  L S +    LLS        L K 
Sbjct: 1081 RQSLPMQWLHRPLLEGRLSLNYECFKTDFKEQDIKTLPSHLCTSALLSKM--VLIALRKE 1140

Query: 1389 TKNEVTSRAWLVAEILCTWKWPGGHARGS-FLPLFCAYV-KRSCSHES---------LLD 1448
            T  E      ++AE+L + +W         FL  FC  + K + ++++         LL 
Sbjct: 1141 TVLENNELEKIIAELLYSLQWCEELDNPPIFLIGFCEILQKMNITYDNLRVLGNTSGLLQ 1200

Query: 1449 STFNMLLDGALLYSNRTAQSIVNIWPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDK 1508
              FN   +   L+S   A+ I++      S+  D  +P  +   SF F L + N+     
Sbjct: 1201 LLFNRSREHGTLWSLIIAKLILS-----RSISSDEVKPHYKRKESF-FPLTEGNLHTIQS 1260

Query: 1509 ASSLFELLVSRLFIGEAVNIDCLRILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENI 1568
                      + F  + +        P ++ +  + +C TN  F       G  ++  + 
Sbjct: 1261 LCPFLSKEEKKEFSAQCI--------PALLGWTKKDLCSTNGGF-------GHLAIFNSC 1320

Query: 1569 IQNAA--EGWLQRVLLFPSLNEWQLGQDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNIST 1628
            +Q  +  +G L   +L   +  W+  ++ ED  + + SC                 N+S 
Sbjct: 1321 LQTKSIDDGELLHGIL-KIIISWK--KEHED--IFLFSC-----------------NLSE 1380

Query: 1629 EEGSLLLELFRKQRKTSGRSPAVNHAPSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQL 1688
                +L                      V + +   + + + YC     + +W+F++  +
Sbjct: 1381 ASPEVL---------------------GVNIEIIRFLSLFLKYCSSPLAESEWDFIMCSM 1440

Query: 1689 MSWIQSAVVIMEEIAESVNDIIVKSSTAMNLNEILEKLEQSVLISDPIPFCISRNALLSF 1748
            ++W+++                       + N+ L  +    L +     C+S +     
Sbjct: 1441 LAWLETT----------------------SENQALYSIPLVQLFA-----CVSCDLACDL 1500

Query: 1749 SLFYGSLGLQGLKDMESSSPHRLDKLNHLNDRIFEAILRMFFCTGISEAIACSCSDKAAS 1808
            S F+ S  L  + ++     + + +      +   ++L     T   E    S +    +
Sbjct: 1501 SAFFDSTTLDTIGNL---PVNLISEWKEFFSQGIHSLLLPILVTVTGENKDVSETSFQNA 1560

Query: 1809 IISSSRLELPYFWDLIASSVTKSSKDARERALKSIEFWGLSKGPVSSLYGILFSHKPVPS 1868
            ++      L Y       S    ++   ++     E+       ++ L  +LF  +PV  
Sbjct: 1561 MLKPMCETLTYISKEQLLSHKLPARLVADQKTNLPEYLQTLLNTLAPL--LLFRARPV-- 1620

Query: 1869 LQYAAYVMLSTEPISYSAIIQENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEIL-LMI 1928
             Q A Y ML           Q+N   Y D +   E+ +     +    + ++E++L  ++
Sbjct: 1621 -QIAVYHMLYKLMPELPQYDQDNLKSYGDEE---EEPALSPPAALMSLLSIQEDLLENVL 1680

Query: 1929 EKLP-DDVFDMELIAQE---RVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSAS-SR 1988
              +P   +  ++ ++++    +   L W L+L+   +   SS  R     Y++ + S ++
Sbjct: 1681 GCIPVGQIVTIKPLSEDFCYVLGYLLTWKLILTFFKA--ASSQLRALYSMYLRKTKSLNK 1740

Query: 1989 ILDCLFQHIPIEGMALQKKKDTELPAGLSEAATAAN---QAITTGSLLFSVEFLWPVEPM 2048
            +L  LF+ +P            E P     A    N   +   T  L  S+     + P 
Sbjct: 1741 LLYHLFRLMP------------ENPTYAETAVEVPNKDPKTFFTEELQLSIRET-TMLPY 1765

Query: 2049 KLASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSSVIESFTKAWCSPSLIANELFQIKKA 2108
             +   A +++ + ++ LPA VR W++   ++   ++++ FT  + S  L   E+  ++ +
Sbjct: 1801 HIPHLACSVYHMTLKDLPAMVRLWWNS-SEKRVFNIVDRFTSKYVSSVLSFQEISSVQTS 1765

Query: 2109 EFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSSYPLRHVDVECMRSLGISEVKQR 2152
                   +V    +  EV+ATYT ++  ++L+I+LPS+YPL  + VE  + +G++  + R
Sbjct: 1861 TQLFNGMTVKARATTREVMATYTIEDIVIELIIQLPSNYPLGSIIVESGKRVGVAVQQWR 1765

BLAST of Clc03G00220 vs. ExPASy Swiss-Prot
Match: Q555H8 (E3 ubiquitin-protein ligase listerin OS=Dictyostelium discoideum OX=44689 GN=rnf160 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 1.6e-71
Identity = 462/2062 (22.41%), Postives = 848/2062 (41.13%), Query Frame = 0

Query: 249  MGRPKGDGAR-SKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGD------D 308
            MG+ +  G R +K  PSSS  A +L+ S S+       GF+G     S+LT         
Sbjct: 1    MGKDRDKGFRGAKYNPSSSGEANTLMGSQSS-------GFIGFSAFSSNLTNATDPDYYQ 60

Query: 309  ATPFSVWLFKRVATSHYYFQQLKALASLSELLKQKSGKD----VASIIP---QWVFEYKK 368
              P    L K++        ++K L  L+   ++ + +D    ++SI P    W F YK+
Sbjct: 61   VEPEYKVLLKKLQKKD-SISRIKGLEELNSKFQKINIEDAEFNISSISPLMNAWEFMYKR 120

Query: 369  LLMDYNRDVRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQA 428
            L+ D +R+VR      + ++    G+ + PH+K L+GPW  +  D   +   +A+ S Q 
Sbjct: 121  LVNDDDRNVRDLASQCLGSIGSRIGKHLGPHVKQLLGPWIVAICDQNDQSINNALLSFQN 180

Query: 429  AFPAQEKRVDALILCTSEIFMYLEENLKLTPDTLSD-KVVAKDELEEMHQQVISSSLLAL 488
             FP ++KR D       E   YL ENL+ TP T+ D K ++++ L+E +++ IS+SLLA+
Sbjct: 181  IFP-EKKRKDVFKFGHDEALTYLCENLQETPQTIGDSKSISQEILQERYERCISNSLLAI 240

Query: 489  ATLIDVLVSVRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTM 548
              LI+   +  ++ S +  SS      + + +G T I   EK+F     F    +SKS+ 
Sbjct: 241  EYLINNTTATATDSSSS--SSTTTTTTTTNNTGNTEI--YEKIF--ESQFFSFFQSKSSN 300

Query: 549  VRSATYTVLRSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRL-P 608
            +R   Y VL +++K +   + E+N K  +  +LG F E+D S H  +WD ++ F ++   
Sbjct: 301  IRKTAYRVLTTVIKKLA-GYVEKNFKDFSSKVLGLFSEKDSSTHLYMWDAIISFLQQYGD 360

Query: 609  NCWTYVNVQKTVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEK--------- 668
              W+ V+V+K VL R W FLR+GC+GS E+SYP+++  L  +P   V G           
Sbjct: 361  KAWSNVDVRKHVLPRLWAFLRSGCYGSFELSYPSILPLLTFIPNSIVIGSPSPSPSPSPN 420

Query: 669  ------FLLDFFENLWVG----------RNPFHSSRVERLAFFQAFKECFL-----WG-- 728
                  F  + F NL  G           NP    R   L     + EC +     WG  
Sbjct: 421  TNIDIGFFKELFTNLEKGLEVVDNPRQYGNPNSVDRPSNL-LLSCYLECLIYSSKKWGQQ 480

Query: 729  IRNASSHFIQNWTF------------CNGDDLAHFQVTLVDVILVKLLWKDYLHVQCL-- 788
             +  + + I    F            C  + L   ++    + L  L   + L +Q L  
Sbjct: 481  YQQINDYLIDTLLFNFYNNHFNLEHSCFSEKLFIEKLLESTIKLANLSNSNLLKIQNLFN 540

Query: 789  -------ENQDRAFSEDAPL-----NDNMAEEIPSIKYPMSYLQDLRK-CIVEILSGIHL 848
                    N++   + + P+     N+N     P IK     +Q + K  ++  L+   +
Sbjct: 541  SNSFINFNNKENLLNNNEPITTDNNNNNNNNSSPIIKKQEKIIQPISKETLISRLNTFII 600

Query: 849  V-----------KHDLLSVFSMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSV 908
            V            ++++ V         ++  +L  +  ++ + ++ I+G     + +S 
Sbjct: 601  VCSNNNNSNNSNNNNVIQVIIKRLFN--LACDNLNSNQELSIKILDFILGNYKIDQIISN 660

Query: 909  DKDDTWPLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSS 968
              DD   ++  +   L  +      L+   G  L+S  V +        AL    + +  
Sbjct: 661  GLDDIKKIITQLALALNESLLSTGGLNLGVGGDLVSVIVGLL-------ALVPDEDEIK- 720

Query: 969  TQFSGVEAQDVEASQFMQLFNEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVI 1028
                      +  SQ +Q+F +         NN++ S + +    LI +   ++    +I
Sbjct: 721  ----------LIWSQILQIFLDSI------NNNNNNSKKKNNRSNLITNT--TNSLVHLI 780

Query: 1029 SYSTNLDHTEVVLESMNSESLAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHL 1088
            + ++N  +  ++L +   +S+    K+ D+ ++++  S +          N  N +NE  
Sbjct: 781  NSASNKINRNLLLSTTLFKSI----KIQDKKQLQLIKSILFN--------NNNNNNNEFS 840

Query: 1089 -----ESAAVAIAQSYAPFRIIISLM-------RSSVLGGSLQNDCYS---FVSRDALIA 1148
                 ES  + I   Y     II+ M         +  GG   ND  S    VS D L+ 
Sbjct: 841  PIIPNESVELLIKWVYNKLVDIINTMVQLNLVFNDNDKGGDNNNDDDSDDNDVSIDYLVE 900

Query: 1149 IFEAVF-QKLVSFLLHSPLIWARNSSSFLISRPENSFPKSTSSSEVVAMAHFALEVLDRC 1208
            + + +F  +  S  L S LI     +  + +RP  S  KS  +S            +D  
Sbjct: 901  LSKLLFSSRFKSKSLESLLIEWCKFNIEINNRPSLSGFKSKCNS------------IDEY 960

Query: 1209 IFCLYNLGEENYLLPSILATIYAIDWDCSMERRQDDMLDDKFKEERRAGLLFGECVRALR 1268
            +F   +  E +  + S+L  I +ID + S     D+ L+  F       L+     R   
Sbjct: 961  LFSTTSNIENDKFILSLL--IESIDRNSSTIITNDN-LNLNFLSNCAIKLIKSGSYRHDE 1020

Query: 1269 QKITDQFWKSCRTHDRKKYGSILIQFIRSAIFSEDSEEIGSLCCQW----MLEILDQI-- 1328
                D         D + Y   + +F  + +     ++I     ++       +LD    
Sbjct: 1021 NGDDDD-------DDDQLYSYFIKEFTSNQLVINSKDQIKISWFRYNRITPFYLLDNYGS 1080

Query: 1329 SQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASNMKNVGLDIHKSGNHKLVSLVS 1388
            ++D+ +        L+K   + F +   F     +  SN +N              S+  
Sbjct: 1081 NKDNNDSTTFSTDKLLKVLIFNFKVIESFGLEKFIKYSNKENNN------------SIEL 1140

Query: 1389 LLMSKIGIEKLLSGQGENSSCLGKTTKNEVTSRAWLVAEILCTWKWPGGHARGSFLPLFC 1448
            LL++      LL   G+N   L  T     T+   +   ++ +                 
Sbjct: 1141 LLLN------LLFVNGDNQ--LPPTPTTTTTTTTTITTAVVVS----------------- 1200

Query: 1449 AYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNIWPYPVSLLEDIQEPFLRALASF 1508
                   ++E +L+           + +N T            +LL    E   +     
Sbjct: 1201 -------TNEQILE----------FIENNST------------NLLNQFIESLFKFYGDE 1260

Query: 1509 L-----FSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDCLRILPL-IVSYLVRPMCET 1568
            +     F+ +  N+ G      ++ +L++ LFI    +  CL    + + S L+  +   
Sbjct: 1261 IFNCNWFNCLVSNVGGDGNNGGIYSVLLA-LFIDYITSDACLPTSSMKLKSALLNSIKID 1320

Query: 1569 NFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLGQDMEDWLLLVMSCYPF 1628
            N   DD+G+   ++  + N++Q          +L P L +  + +  + W +  +S    
Sbjct: 1321 N---DDNGNSI-DSIRQLNLVQ----------ILLPILQKESI-ESYKQWSINQLSQLS- 1380

Query: 1629 SSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHAPSVQMLLSE--LMVVS 1688
            SS     +++ +  +I +   S++++  ++Q + S +          ++L  +  L +++
Sbjct: 1381 SSIPTNFKSISIQLSILS---SIVIQRQQQQHQQSQKEQQQITQDDNEILKEKEWLTLLN 1440

Query: 1689 VGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAES-VNDIIVKSSTAMNLNEILEKL- 1748
            +    +Q++ +    ++ Q    ++   + M+E+  S +N I++      N+N    K  
Sbjct: 1441 LIQTIQQWNSKIKSAIIKQQEPELE--FLFMKEVELSCLNFILIVIQYISNINSNKSKQP 1500

Query: 1749 -----EQSVLISDPIPFCISRN-ALLSFSLFYGSLGLQGLKDMESSSPHRLDKLNHLNDR 1808
                  QS  +   +   +S N  LLSF+ F  +   Q  +++      ++  +   ND 
Sbjct: 1501 IYLSNNQSQFVQSFLSIWLSNNFTLLSFNKFKSNYYYQQYRNLIEYQSLKIYNIIISNDN 1560

Query: 1809 IFEAILRMFFCTGISEAIACSCS----DKAASIISSSR--------LELPYFWDLIASSV 1868
            I       F   GI +      S    +   + +S+SR         +  Y  +L +++ 
Sbjct: 1561 I------KFIIPGIGDQFQIDHSILILNNWLNRVSNSRSNNNNGNTSDSSYILELYSNTF 1620

Query: 1869 TKSSKDARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAII 1928
             K S D +         + +S   + +L  +L +     S+     +  ++  +  S   
Sbjct: 1621 MKMSNDIK---------YKISLTKLDNLIELLSTFNYSTSIYNNKGIKKTSYNLLESYFG 1680

Query: 1929 QENTSCYLDYDITTE---------QGSTQVDFSSEYNVLLKEEILLMIE---KLPDDVFD 1988
             ENT     + +  E          G  +V F    N+   E+ILL  +   K      D
Sbjct: 1681 NENTQLVKSFLLENENNNNNNNNDDGEEKVSFILFSNIF--EKILLDFDSTFKPMKKSKD 1740

Query: 1989 MELIAQERVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGM 2048
             +LI    +   + W+LLL +   +    + R  L  ++ ++   ++   L Q  P    
Sbjct: 1741 NQLINSISMGQLMCWNLLLLNYSKI--DLNRRSILNGWLNDNNIQKVTLFLNQIFP---- 1800

Query: 2049 ALQKKKDTELPAGLSEAATAANQAITTGSLLFSVEFLWP-------VEPMKLASFAGAIF 2108
                K ++ L    +      N         +S+EF +        +    +       F
Sbjct: 1801 -FINKDESPLTTTTTTTDNNGNN--------YSIEFPFSIIDNDQLITYRMIQKLCSNCF 1860

Query: 2109 GLMIRVLPAYVRGWFSDLRDRSK-SSVIESFTKAWCSPSLIANEL-----FQIKKAEFAD 2150
              ++  LP+ VR W  D    SK +  I+S+   + SP +I NE+     ++   ++ +D
Sbjct: 1861 YSLVNYLPSMVRRWADDNSTNSKMNQQIQSYITRFISPIIIQNEIQRVNSYRCTDSDSSD 1861

BLAST of Clc03G00220 vs. ExPASy Swiss-Prot
Match: E1C231 (E3 ubiquitin-protein ligase listerin OS=Gallus gallus OX=9031 GN=LTN1 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 6.0e-71
Identity = 451/2007 (22.47%), Postives = 827/2007 (41.21%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGF-------------LGSYRLDS 308
            MG       +   RPSSS  AA LL  +    V GF GF              G+  +DS
Sbjct: 1    MGGKNKQRTKGNVRPSSSGRAAELLAKE-RGTVPGFIGFGTSQSDLGYVPAVQGAEEIDS 60

Query: 309  SLTGDDATPFSVWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKL 368
             +  D    F + L  R  +      +LKA+     + K++  + V  ++P W   Y K+
Sbjct: 61   LVDAD----FRMVL--RKLSKRDIITKLKAMQEFGTMCKEREAEVVKGVLPYWPRIYCKI 120

Query: 369  LMDYNRDVRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAA 428
             +D++R VR AT  +   L++   + +AP+LKS+MG W  +Q D+ S  + +A ++ + A
Sbjct: 121  SLDHDRRVREATQQSFEQLILKVKKHLAPYLKSIMGYWLIAQCDTYSPAASAAKEAFEKA 180

Query: 429  FPAQEKRVDALILCTSEIFMYLEEN-LKLTPDTLSD-KVVAKDELEEMHQQVISSSLLAL 488
            FP+  K+ +AL  C  EI   L+++ LK TPDTLSD + V ++E E    ++++ SLLAL
Sbjct: 181  FPS-SKQPEALAFCKDEILNVLQDHLLKETPDTLSDPQTVPEEEREAKFFRILTCSLLAL 240

Query: 489  ATLIDVLVSVRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTM 548
              L+ +L           K    ++    S            L +++K++    K  +  
Sbjct: 241  KKLLSMLPK---------KEMHSLEEKLMS------------LLSQNKFW-KYGKHSTPQ 300

Query: 549  VRSATYTVLRSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPN 608
            VRSA + +  +  + +P   K +  + +  ++L +  + D     ++W+ VL     + +
Sbjct: 301  VRSAFFELASAFCQFLPELVKAEAPR-VCPAVLLSIDDSDAVVCPALWEAVLHAIATIED 360

Query: 609  CWTYVNVQKTVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGE-KFLLDFFENL 668
            CW++VN +K VL + W  LR G  G   + YP ++ F+  VPP     + ++   FF ++
Sbjct: 361  CWSHVNARKGVLPKLWTVLREGGRGLATVIYPNILPFISKVPPGITEPKLEYFRTFFSSI 420

Query: 669  WVGRNPFH--SSRVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQ-VTLVD 728
              G +     +S  E  A    F EC  + I        Q         L + Q + L D
Sbjct: 421  IQGLSNERALASPSESSAIITTFMECLRFAILQKIDEDEQRQIH---QMLIYDQLIPLTD 480

Query: 729  VILVK-LLWKDYLHVQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEIL 788
             +L +  L    L  Q  E      ++    +D+   E+        + + L+ CI+   
Sbjct: 481  AVLQEPRLQNGPLFYQIAETLSSWEAKAEVSSDDNTNEVFQKLLSNFWDRLLKMCILH-- 540

Query: 789  SGIHLVKHDLLSVFSMEFQKDCISMFHLTESMGVASETIELIIGFILELE---------- 848
              +  ++ D  ++F++    D + +    ++    +    L + F  E E          
Sbjct: 541  --VDKLEADEKTLFAI---SDMLEVLQNPKTATKPNNRKSLKVKFSDEDESERNTENGKI 600

Query: 849  ---------QLSVDKDDT-----WPLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVF 908
                     ++  D   +      PL  LV      +   +    S   ++ LSA ++ F
Sbjct: 601  TEVRSNSDSEIQADLQHSSILRKEPLENLVCNLAELSIVYVNEQKSEQHLKFLSALLNFF 660

Query: 909  GPRKIVQALFIHNNGMSSTQFSGVEAQDVEASQFMQLFNEVFVPWCLQGNNSSASARLDL 968
               ++ Q L          Q S       E+ + M++ NE         N S     ++L
Sbjct: 661  SSNRVFQVLL--------EQGSNAGCPPAESQEDMKVHNE---------NPSVQFLYMNL 720

Query: 969  LLELID----DEHF-SDQWHSVISYSTNLDHTEVVLESMNSESL--AVLAKLLDRARVKI 1028
            +  L +    D HF  D  +SV++   + D  +V+L+ +    L   V  +++ +A    
Sbjct: 721  ITWLKEDWRKDMHFLVDILYSVLNCCNSDDERKVILDDLTKMDLKWIVFLQIIQKA---C 780

Query: 1029 TNSDVRKLTHTWQKANL---------GNWHNEHLESAAVAIAQSYAPFRIIISLMRSSVL 1088
            +++    L   W K ++          +  +  L+  A +   S +    ++SL+ S   
Sbjct: 781  SSTTKLSLISEWLKGDMLGERLVMLADDLCHLGLKPIATSPESSSSEKWTVLSLVLS--- 840

Query: 1089 GGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISRPENSFPKSTS 1148
                       +  ++LI   E   ++++   L + L  A++ S    + P  SF    +
Sbjct: 841  ---------QHIKNESLIG--ETYVERIID-KLQAALSKAKDLSEAGNTEPSVSFICDVA 900

Query: 1149 SSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERRQD------D 1208
            SS   ++             CL     E+ LL     TI+ +   C+  +RQD      D
Sbjct: 901  SSFFSSVKG-----------CLLMPSSEDLLL-----TIFQL---CA--QRQDATHLTVD 960

Query: 1209 MLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFSEDS 1268
            +L  K K    +G+     VR LR        +S   H             +SA++ ++ 
Sbjct: 961  LLVCKLKHTWISGV--NSLVRHLRSMQN----QSTFLH-------------KSALWIKNQ 1020

Query: 1269 EEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAP--DFMAPNELAASNM 1328
             +  SL  + +  ++  +S        +L  LL  D +    +    + + PN      +
Sbjct: 1021 IQSSSLDVKSLQVLISAVSD-------LLSTLLEADRQSGCLVGAYVEHVMPNRTEWETL 1080

Query: 1329 KNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSSCLGKTTKNEVTSRAWLVAEI 1388
            +               SL +  M K  +E  LS    N   LG   K             
Sbjct: 1081 RE--------------SLSAEWMHKPLLEGRLS---MNCEHLGSCVK------------- 1140

Query: 1389 LC-TWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNIW 1448
            LC T K PG     + L      V  +   +   D+    +L   LLYS +  + + N  
Sbjct: 1141 LCGTTKLPGHLCTSALLSKMVLLVLENDMVKGNEDAE---ILVAELLYSLQWIEELENP- 1200

Query: 1449 PY----PVSLLEDIQEPFLRALASFLFSLVKENIWGR-DKASSLFELLVSRLFIGE-AVN 1508
            PY     + +LE++   + +  A    + +++ I+ R ++   L+ L ++++  GE AV+
Sbjct: 1201 PYLLLEYLHMLEEMHITYEKFSALSNTTNLQQTIFDRSEEHGRLWSLTMAKVIRGENAVS 1260

Query: 1509 IDCLRI-------LPLIVSYLVRPMCETNFTFDDSG-----SCPGEASLKENIIQNAAEG 1568
             +  ++       LPL    L    C + F  ++        C  +         ++ +G
Sbjct: 1261 REMTKLFKTSEGFLPLTEGRLHTLQCLSPFLIEEEKRELVFHCVAKLMTCTQTELSSTDG 1320

Query: 1569 WLQ-RVLLFPSLNEWQLGQD--MEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLL 1628
                  +L  SLN+   G D  +   L +++S                    +  E S L
Sbjct: 1321 AFGCLAILNSSLNDKSFGCDHLLPGVLKIIISWK------------------NDNEDSFL 1380

Query: 1629 LELFRKQRKTSGRSPAVNHAPSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQS 1688
                 K+  TS +    N    ++M+    + + + Y      D +W+F++  +++W++ 
Sbjct: 1381 FSCNLKE--TSAQLLGFN----IEMI--RYLPLLLKYSTAPLADNEWDFIMCSMLAWLE- 1440

Query: 1689 AVVIMEEIAESVNDIIVKSSTAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGS 1748
                               +T+ N +     L Q       I  C+S +   + S ++  
Sbjct: 1441 -------------------TTSENYSLYHVPLVQ-------IFACVSCDLASALSAYFEP 1500

Query: 1749 LGLQGLKDMESSSPHRLDKLNHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSR 1808
                  K  E+   + + +           +L   F     E    +      S+++S  
Sbjct: 1501 ---AAPKTTENLPVNLVSEWKEFFSEGIHNLLLPLFVKVTGETKTAAEGSFQNSVLTS-- 1560

Query: 1809 LELPYFWDLIASSVTKSSKD-ARERALKSIEFWGLSKGPVSSLYGILFSHKPV-----PS 1868
                     +  ++T  SKD      L +    G       +L  +L +  P+      S
Sbjct: 1561 ---------LGEALTYISKDQLLNHKLPAKFVAGQKTNLPDNLQTLLNTLSPLLLFWARS 1620

Query: 1869 LQYAAYVMLSTEPISYSAIIQENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILL--M 1928
            +Q + Y MLS           E+   Y D     E+       ++  +VL  +E+LL  +
Sbjct: 1621 VQVSVYHMLSKLMPELPKFDDEDLKSYGD----EEEELALSPPAALMSVLATQELLLENI 1680

Query: 1929 IEKLPDDVFD-MELIAQE---RVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSAS-S 1988
            +E +P   F  ++ ++ E    +   L W L L+   +   SS  R    QY++ + S +
Sbjct: 1681 LECIPVGEFAVIQPLSDEFCLVLGYLLTWKLTLTFFKA--ASSQLRVLYSQYLRRTKSLN 1740

Query: 1989 RILDCLFQHIPIEGMALQKKKDTELPAGL-SEAATAANQAITTGSLLFSVEFLWPVEPMK 2048
            ++L  LF+ +P          +  + +GL SE      +   T  L   V+    +   +
Sbjct: 1741 KLLYHLFRLMP----------ENPVFSGLTSEVPNKDTKTFFTEELHLDVKGTGALS-SQ 1765

Query: 2049 LASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSSVIESFTKAWCSPSLIANELFQIKKAE 2108
            +   A +++ + ++ LPA VR W++    R   +V++ FT  + S  L + E+  ++ + 
Sbjct: 1801 IPHLACSVYHITLKDLPAMVRLWWNSCEKR-VFNVVDKFTSKYVSSVLSSQEISSVQTST 1765

Query: 2109 FADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSSYPLRHVDVECMRSLGISEVKQRK 2152
                  +V    +A EVIATY+ D+  ++L+I+LPS+YPL  + VE  + +G++  + R 
Sbjct: 1861 QLFNGMTVKARSAAREVIATYSVDDIFIELIIQLPSNYPLGSITVESGKRVGVAVQQWRN 1765

BLAST of Clc03G00220 vs. ExPASy Swiss-Prot
Match: A0JM49 (E3 ubiquitin-protein ligase listerin OS=Xenopus tropicalis OX=8364 GN=ltn1 PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 1.8e-70
Identity = 427/1922 (22.22%), Postives = 780/1922 (40.58%), Query Frame = 0

Query: 332  LLKQKSGKDVASIIPQWVFEYKKLLMDYNRDVRRATHDTMTNLVMAAGREIAPHLKSLMG 391
            + K++  + V  ++P W   Y K+ +D +R VR AT      L++   + +APHLKSLMG
Sbjct: 7    MCKEREMETVRGVLPYWPRIYCKISLDLDRRVREATQQAFEQLILKVKKYLAPHLKSLMG 66

Query: 392  PWWFSQFDSVSEVSQSAMQSLQAAFPAQEKRVDALILCTSEIF-MYLEENLKLTPDTLSD 451
             W  +Q D+ S  + SA  + +AAFPA  K+ +AL  C  EI  + L+  LK TPDTLSD
Sbjct: 67   FWLIAQCDTYSPAASSARVAFEAAFPA-HKQPEALAFCKEEIMNVLLDHLLKETPDTLSD 126

Query: 452  -KVVAKDELEEMHQQVISSSLLALATLIDVLVSVRSERSGTGKSSGEIKHASKSRSGETA 511
             + V  ++ E  + +V++ SLLAL  L+ +L   RSE S   +    +   SK       
Sbjct: 127  TQAVPIEDRESKYFRVVTCSLLALKRLLCML--PRSENSSLQERLAHLLSQSK------- 186

Query: 512  IAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVLRSLVKNIPHAFKEQNMKTIAGSILGAF 571
                         F    K  ++ +RSA + ++ +L +  P   + +  + +  ++L + 
Sbjct: 187  -------------FWKYSKHSTSSIRSAFFELMSALCQCSPDVLRAEASR-LCPAVLLSI 246

Query: 572  QERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQEISYPALIL 631
             + D     ++W+ VL     + +CW +VN QK VL + W+ LR G  G   + +P L+ 
Sbjct: 247  DDSDAVVCPALWEAVLYTITTIEDCWEHVNAQKGVLPKLWSVLREGGRGLATVIFPNLLP 306

Query: 632  FLDTVPPHAV-GGEKFLLDFFENLWVGRNPFH--SSRVERLAFFQAFKECFLWGIRNASS 691
            F+  VP + +     F  +FF +L  G +     +S  E  A   A+ EC  + ++  + 
Sbjct: 307  FISRVPTNIIKTPTDFYSNFFNSLGQGLSSERAVASPAECSAIISAYMECLRFAMQENAG 366

Query: 692  HFIQN-----------------------------------WTFCNGDDLAHFQ-----VT 751
               +N                                    T  + +  A  Q     ++
Sbjct: 367  EEEENKKVQQILISDQLMPLIDSSLKDPKLQNSPLFSQVEETLYSWEKKAESQGLDDKLS 426

Query: 752  LVDVILVKLLWKDYLHVQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVE 811
             +   L++  W++  H+ C+ + D        + +   + +  +   +  LQ+   C+++
Sbjct: 427  RIHATLLREFWENLAHI-CVSHVD--------VIEAGEKNLAGVSGLLQVLQN-PNCLLK 486

Query: 812  ILSGIHLVKHDLLSVFSMEFQKDCISMFHLTESMGVASETIELI--IGFILELEQLSVDK 871
            +       K      F  E   +  ++ H++ES  ++   +E     G    + QL  ++
Sbjct: 487  VNK-----KKKAKIRFKGEGDDESDAVSHVSESQPMSKFIMETCSSSGRDPGIAQLRQER 546

Query: 872  DDTWPLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQ 931
                 L  LV      +   I    S   +  L+A +S F   ++ Q L   ++      
Sbjct: 547  -----LEDLVCKLAELSMVYISEQRSQQHLMFLAALLSTFPSIRVFQVLLQQSSEEDPKN 606

Query: 932  FSGVEAQDV---EASQFMQLFNEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSV 991
             +  +  DV     +  +Q      + W     N  +    D L++++         +SV
Sbjct: 607  LNSPD-HDVTPRHKNPAVQFLYAKLIFWF----NDKSLEESDFLVDIL---------YSV 666

Query: 992  ISYSTNLDHTEVVLESMNSESL--AVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHN 1051
            +   T+    + +L+ M   +   +V   ++ +A    T+ +   L   W K   G    
Sbjct: 667  LFCCTDSSERKHLLDDMTKMNFKWSVFLYIIQKA---CTDPEKYSLASDWLK---GEVLG 726

Query: 1052 EHLESAAVAIAQSYAPFRIIISLMRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSF 1111
            E L   A  +  S    +   S    S     L       V  + LIA  +A  +K+++ 
Sbjct: 727  ERLICLANDLCTSGLMDKATPSEAYCSKKWALLSLVLSEHVHNEYLIA--DAYVEKIIN- 786

Query: 1112 LLHSPLIWARNSSSFLISRPENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYL 1171
             LHS L+ ARN S         SF     S+   ++             CL     E+ L
Sbjct: 787  KLHSALMKARNLSKAGHLEQSVSFICDVGSNFFSSIKG-----------CLQMPSAEDLL 846

Query: 1172 LPSILATIYAIDWDCSMERRQDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCR- 1231
            L     TI+ +    S      + L  K K    AGL    C      + ++   +S + 
Sbjct: 847  L-----TIFQLCAQASDTSHMSESLLLKLKNTWLAGLHSLVCQYKNMPQGSNFLKQSAQW 906

Query: 1232 THDRKKYGSILIQFIRSAIFSED---SEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLL 1291
              D+ +   + ++ ++S I + D   S  + S    + L      S     E +   +  
Sbjct: 907  VKDQIQTSHLNVKSLQSLICTVDDLYSTILDSSLSGFSLLAEHTASMMPSAETWQKMRHA 966

Query: 1292 IKDDKWPFWIAPDFMAPNELAASNMKNVGLDIHKSGNHKLVS--LVSLLMSKIGIEKLLS 1351
            +       W++   +      + N +  G D+       L S    + L+SK+ +  L  
Sbjct: 967  LSSQ----WLSKPLLEGR--LSMNTETSGTDLKSFPKTWLPSHLCTASLLSKMALRLLEK 1026

Query: 1352 GQGENSSCLGKTTKNEVTSRAWLVAEILCTWKWPGGHAR-----GSFLPLFCAYVKRSCS 1411
             +      +G   K  +T     VAE+L + +W     +     G    + C+      S
Sbjct: 1027 EKSLKEEEIG--VKINIT-----VAEMLYSLQWCEELEKPPTLIGECCEMLCSL---GVS 1086

Query: 1412 HESLLDSTFNMLLDGALLYSNRTAQSIVNIWPYPVS---------------LLEDIQEPF 1471
            HE ++D + ++     LLY NR+ +   ++W    +               L ED ++ F
Sbjct: 1087 HEKVMDLSVHLPGLLELLY-NRSKED-GSLWSLTANRFIHKRGAGPSELLPLTEDTEKYF 1146

Query: 1472 ---------LRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDC----LRIL 1531
                     +++L++FL   +KE          L     +RL    A  + C       L
Sbjct: 1147 PVTLGSLHTIQSLSAFLCPELKE---------ELVIQCTARLMTCSASAVSCTDGGFGYL 1206

Query: 1532 PLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLGQD 1591
             +I                   SC G  S   ++ Q    G L+ +L       W+   D
Sbjct: 1207 AII------------------NSCLGSDS---DLCQEILPGVLKVML------SWK--DD 1266

Query: 1592 MEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHAPS 1651
             ED  L   +    S S+ GL  +++ R +     S+LL+                 A S
Sbjct: 1267 QEDIFLFSSNVQDSSQSLIGL-NVEMIRFL-----SILLKYL---------------ASS 1326

Query: 1652 VQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIVKSSTA 1711
            V  L+                D DW+F++  +++W++SA                +SS A
Sbjct: 1327 VSSLV----------------DVDWDFIMCSMLAWLESA---------------CESSAA 1386

Query: 1712 MNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRLDKLNH 1771
             ++  +             +  C S +     S ++ S+       + ++      +   
Sbjct: 1387 YHIPLV------------RLFACASCDLATEISHYFESVATSTTMSLPANLMSEWKEF-- 1446

Query: 1772 LNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDAR 1831
             ++ I+  +L M     I+E    S +   + ++ S    L Y           S +   
Sbjct: 1447 FSEGIYSLMLPML--VRIAEEYKASETSHMSHVLKSLGRTLGYI----------SKEQLL 1506

Query: 1832 ERALKSIEFWGLSKGPVSSLYGILFSHKPV-----PSLQYAAYVMLSTEPISYSAIIQEN 1891
               L +    G       SL  +L +  P+      S+Q   Y +L            E+
Sbjct: 1507 NHKLPAKFVAGQKTNLTDSLQSLLNTLAPLLLYRERSVQITVYHLLDKIMADLPGFDDED 1566

Query: 1892 TSCYLDYDITTEQGSTQVDFSSEYNVLLKEEIL-LMIEKLPDDVFD-MELIAQE---RVN 1951
               Y D D   E+ +     +    + ++E+++  +++++P   F  +E + +E    + 
Sbjct: 1567 LKSYGDED---EEPALSPPAALMSVLAIQEDLVESILKEIPVGEFAVIEPLKEEFCFVLG 1626

Query: 1952 IYLAWSLLLSHLWSLPPSSSARERLVQYIQNSAS-SRILDCLFQHIPIEGMALQKKKDTE 2011
              L W L+L+   +   SS  R    QY++ + S +++L  LF+ +P          +  
Sbjct: 1627 YLLTWKLILTFFKA--ASSQLRALYSQYLRKTKSLNKLLYNLFKLMP---------HNPV 1686

Query: 2012 LPAGLSEAATAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFS 2071
            LP   S+  +   +   T  L  +V+    V+  ++   A  ++ + ++ LPA VR W++
Sbjct: 1687 LPGQASDMPSKEPKTFFTEELQLAVKGTATVQ-QEVPHLACCVYHMTLKDLPAMVRLWWN 1695

Query: 2072 DLRDRSKSSVIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDE 2131
                R   +V++ FT  + S  L + E+  ++ +    +  +V    +  EVIATY+ D+
Sbjct: 1747 GCEKRI-FNVVDRFTTKYVSSVLSSQEISSVQLSTQVFDGMTVKARSTTREVIATYSVDD 1695

Query: 2132 TGMDLVIRLPSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 2152
              ++L+I+LP +YPL  + VE  R +G++  + R W+L + +++ +QNG++ E + +WK 
Sbjct: 1807 ICIELIIQLPQNYPLGSITVESGRRVGVAVQQWRNWMLQLNTYLTHQNGSIMEGLALWKN 1695

BLAST of Clc03G00220 vs. ExPASy TrEMBL
Match: A0A1S3C4S0 (E3 ubiquitin-protein ligase listerin OS=Cucumis melo OX=3656 GN=LOC103497027 PE=3 SV=1)

HSP 1 Score: 3332.0 bits (8638), Expect = 0.0e+00
Identity = 1691/1913 (88.40%), Postives = 1777/1913 (92.89%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MGRPKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGSYRLDSSLTGDDA PFS  
Sbjct: 1    MGRPKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSSLTGDDAAPFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE+LKQKSGKDVASIIPQWVFEYKKLLMDYNRD
Sbjct: 61   DSEVAQHLKRLSRKD-PTTKLKALASLSEILKQKSGKDVASIIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDAL+LCT+EIF+YLEENLKLTPDTLSDK+VAKDELEEMHQQVISSSLLALATLIDVLVS
Sbjct: 181  VDALVLCTTEIFIYLEENLKLTPDTLSDKLVAKDELEEMHQQVISSSLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
             RSERSGTGKSSGE KH S SRS ETAI+FAEKLFTEHKYF+DLLKSKS +VRSATY+V+
Sbjct: 241  GRSERSGTGKSSGETKHTSMSRSRETAISFAEKLFTEHKYFIDLLKSKSNIVRSATYSVM 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            RSLVKNIPHAFKEQNMKTIAGSILGAFQE+DPSCHS +WD VLLFSKRLPNCWTYVNVQK
Sbjct: 301  RSLVKNIPHAFKEQNMKTIAGSILGAFQEKDPSCHSPMWDAVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ+ISYP LILFLDTVPP AVGGEKFLLDFFENLWVGRNPFHSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQKISYPTLILFLDTVPPCAVGGEKFLLDFFENLWVGRNPFHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQVTLVDVILVKLLWKDYLH 728
              ERLAFFQAFKECFLWGI+NASS       FCNGDD  HFQVTL+D ILVKLLWKDYLH
Sbjct: 421  STERLAFFQAFKECFLWGIQNASS-------FCNGDDFVHFQVTLIDAILVKLLWKDYLH 480

Query: 729  VQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVKHDLLSVF 788
            VQCL+NQDR FSEDAPLN+ M E++PS KYPMSYLQDLRKCIVEILS IHL KHDLLSVF
Sbjct: 481  VQCLKNQDRVFSEDAPLNNKMTEDLPSTKYPMSYLQDLRKCIVEILSSIHLAKHDLLSVF 540

Query: 789  SMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTWPLVLLVGPTLANTF 848
            +MEFQK+CI MF LT+++GVASETIE IIGFILELEQLS+DKDDTW LV LVGPTLANTF
Sbjct: 541  AMEFQKNCIDMFQLTDNVGVASETIEQIIGFILELEQLSMDKDDTWLLVHLVGPTLANTF 600

Query: 849  PIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVEAQDVEASQFMQLF 908
            PII+SLDS DGVRLLSAAVSVFGPRKIV+ LFI+NNGMSST+FSGVEAQD+EA QFMQ+F
Sbjct: 601  PIIQSLDSLDGVRLLSAAVSVFGPRKIVRELFINNNGMSSTKFSGVEAQDLEARQFMQVF 660

Query: 909  NEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLESMNSES 968
            N++FVPWCLQGNNSS+SA+LDLLL LIDDEHFSDQWHSVISYSTNL+HTEVVLESMNSES
Sbjct: 661  NDIFVPWCLQGNNSSSSAQLDLLLALIDDEHFSDQWHSVISYSTNLNHTEVVLESMNSES 720

Query: 969  LAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVAIAQSYAPFRIIISL 1028
            LAVLAKLL+R R KITNSD RK+THTWQ+ANLGNWH+EHLESAA+AIAQS+AP R   + 
Sbjct: 721  LAVLAKLLNRVRGKITNSDARKVTHTWQRANLGNWHHEHLESAAIAIAQSHAPIRSSFTD 780

Query: 1029 MRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISR---P 1088
               SVLGGS+QNDC SFVSRDALIAIFEA+FQKLVSFLLHSP  WARNS S LISR   P
Sbjct: 781  FLCSVLGGSVQNDCSSFVSRDALIAIFEALFQKLVSFLLHSPFTWARNSCSLLISRPDSP 840

Query: 1089 ENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERR 1148
            E  FPK T SSEVV MA+FALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSME +
Sbjct: 841  EKIFPKYT-SSEVVVMANFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMEGK 900

Query: 1149 QDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFS 1208
            QDD+LD+KFKEE +A LLFGE VRALRQKITDQFWKSCRTH+RKKYGSILIQFIRSAIFS
Sbjct: 901  QDDVLDEKFKEESKARLLFGESVRALRQKITDQFWKSCRTHNRKKYGSILIQFIRSAIFS 960

Query: 1209 EDSEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASN 1268
            EDSEEI SLC QWMLEILD ISQD  EEQYMLDQLLIK+D WPFWIAPDFMAPNE AASN
Sbjct: 961  EDSEEIVSLCLQWMLEILDHISQDQFEEQYMLDQLLIKNDTWPFWIAPDFMAPNEFAASN 1020

Query: 1269 MKNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSS-CLGKTTKNEVTSRAWLVA 1328
             KN+GLDIH SGNHK +SL+S+ MSKIG+EKL SGQ ENSS C+ K TKNEVTSRAWLVA
Sbjct: 1021 TKNIGLDIHISGNHKFISLISMFMSKIGLEKLFSGQVENSSPCISKMTKNEVTSRAWLVA 1080

Query: 1329 EILCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNI 1388
            EILCTWKWPGG+ARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYS+R AQS++NI
Sbjct: 1081 EILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSSRAAQSLINI 1140

Query: 1389 WPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDCLR 1448
            WPYPVSLLEDIQEPFLRALAS LFSL+KENIWGRDKASS FELLVSRLFIGEAVNIDCLR
Sbjct: 1141 WPYPVSLLEDIQEPFLRALASLLFSLLKENIWGRDKASSQFELLVSRLFIGEAVNIDCLR 1200

Query: 1449 ILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLG 1508
            ILPLI+SYLVRPMCE N TFDD GSC G+ SL EN  Q   EGWLQRVLLFPSLNEWQLG
Sbjct: 1201 ILPLILSYLVRPMCERNSTFDDCGSCSGD-SLMENTFQRTTEGWLQRVLLFPSLNEWQLG 1260

Query: 1509 QDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHA 1568
            QDME WLLLV+SCYPFS S+GGLQTLK+DRNISTEEGSLLLELFRKQRK S RSPAVNHA
Sbjct: 1261 QDMEYWLLLVISCYPFSCSIGGLQTLKLDRNISTEEGSLLLELFRKQRKASSRSPAVNHA 1320

Query: 1569 PSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIVKSS 1628
            P VQMLLSELMVVSVGYCWKQF  EDWEFLL QLMSWIQSAVVIMEEIAESVNDIIVKSS
Sbjct: 1321 PWVQMLLSELMVVSVGYCWKQFSHEDWEFLLFQLMSWIQSAVVIMEEIAESVNDIIVKSS 1380

Query: 1629 TAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRLDKL 1688
            TAM+LNEILEKLEQSVLI DPIPFCISRNALLSFSLF GSLGL GLKDMESSSP + DKL
Sbjct: 1381 TAMDLNEILEKLEQSVLILDPIPFCISRNALLSFSLFDGSLGLHGLKDMESSSPQQFDKL 1440

Query: 1689 NHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKD 1748
            NH+NDRI E ILRMFFCTGISEAIA S SDKAASIISSSRLELPYFWDLIASSVTKSSKD
Sbjct: 1441 NHVNDRIVEGILRMFFCTGISEAIAYSFSDKAASIISSSRLELPYFWDLIASSVTKSSKD 1500

Query: 1749 ARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAIIQENTSC 1808
            ARERA+KSIEFWGLSKGPVSSLYGILFS KP+PSLQYAAYVMLSTEPIS SAII+ENTSC
Sbjct: 1501 ARERAVKSIEFWGLSKGPVSSLYGILFSPKPIPSLQYAAYVMLSTEPISNSAIIRENTSC 1560

Query: 1809 YLDYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQERVNIYLAWSLL 1868
            YLDYDITTEQ STQVDFSSEYNVLLKEEIL MIEKLPDDVF+MELIAQERVNIYLAWSLL
Sbjct: 1561 YLDYDITTEQRSTQVDFSSEYNVLLKEEILCMIEKLPDDVFEMELIAQERVNIYLAWSLL 1620

Query: 1869 LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKDTELPAGLSEAA 1928
            LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIP+EGMALQK+KDTELPAGLSEAA
Sbjct: 1621 LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKRKDTELPAGLSEAA 1680

Query: 1929 TAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSS 1988
            TAANQAITTGSLLFSVEFLWP+EP+KLASFAGAIFGLM+RVLPAYVRGWFSDLRDRSKSS
Sbjct: 1681 TAANQAITTGSLLFSVEFLWPIEPVKLASFAGAIFGLMLRVLPAYVRGWFSDLRDRSKSS 1740

Query: 1989 VIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL 2048
            V+ESFTKAWCSPS+IANEL QIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL
Sbjct: 1741 VLESFTKAWCSPSIIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL 1800

Query: 2049 PSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV 2108
            PSSYPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV
Sbjct: 1801 PSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV 1860

Query: 2109 EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 2152
            EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF
Sbjct: 1861 EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 1903

BLAST of Clc03G00220 vs. ExPASy TrEMBL
Match: A0A0A0LXT2 (E3 ubiquitin-protein ligase listerin OS=Cucumis sativus OX=3659 GN=Csa_1G533710 PE=3 SV=1)

HSP 1 Score: 3321.9 bits (8612), Expect = 0.0e+00
Identity = 1687/1913 (88.19%), Postives = 1775/1913 (92.79%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MGRPKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGSYRLD SLTGDDA PFS  
Sbjct: 1    MGRPKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDYSLTGDDAAPFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE+LKQKSGKDVASIIPQWVFEYKKLLMDYNRD
Sbjct: 61   DGEVAQHLKRLSRKD-PTTKLKALASLSEILKQKSGKDVASIIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDALILCT+EIF+YLEENLKLTPDTLS+KVVAKDELEEMHQQVISSSLLALATLIDVLVS
Sbjct: 181  VDALILCTTEIFIYLEENLKLTPDTLSEKVVAKDELEEMHQQVISSSLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
             RSERSGTGKSSGE KHASKSRS ETAI+FAEKLFTEHKYF+DLLKSKS +VR ATY+V+
Sbjct: 241  GRSERSGTGKSSGETKHASKSRSRETAISFAEKLFTEHKYFIDLLKSKSNIVRFATYSVM 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            RSLVKNIPHAFKEQNMKTIAGSILGAFQE+DPSCHS +W+ VLLFSKRLPNCWTYVNVQK
Sbjct: 301  RSLVKNIPHAFKEQNMKTIAGSILGAFQEKDPSCHSPMWEAVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ+ISYP LILFLDTVPP AVGGEKFLLDFF+NLWVGRNPFHSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQKISYPTLILFLDTVPPRAVGGEKFLLDFFDNLWVGRNPFHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQVTLVDVILVKLLWKDYLH 728
              ERLAFFQAFKECFLWGI+NASS       FCNGDD AHFQVTLVD ILVK+LWKDYLH
Sbjct: 421  STERLAFFQAFKECFLWGIQNASS-------FCNGDDFAHFQVTLVDAILVKILWKDYLH 480

Query: 729  VQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVKHDLLSVF 788
            VQCL+NQDR FSED PLN+ M E+IPS KYPMSYLQDLRKCIVEILS IHLVKHDLLSVF
Sbjct: 481  VQCLKNQDRVFSEDEPLNNKMIEDIPSTKYPMSYLQDLRKCIVEILSSIHLVKHDLLSVF 540

Query: 789  SMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTWPLVLLVGPTLANTF 848
            +MEFQK+C+ MF LT+++GVASETIE IIGFILELEQLS+DKDDTW LV LVGPTLANTF
Sbjct: 541  AMEFQKNCLDMFQLTDNVGVASETIEQIIGFILELEQLSMDKDDTWLLVHLVGPTLANTF 600

Query: 849  PIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVEAQDVEASQFMQLF 908
            PII+SLDSSDGVRLLSAAVSVFGPRKIVQ LFI+NNGMSST+FSGVEAQD+EA QFMQ+F
Sbjct: 601  PIIQSLDSSDGVRLLSAAVSVFGPRKIVQELFINNNGMSSTEFSGVEAQDLEARQFMQVF 660

Query: 909  NEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLESMNSES 968
            N+VFVPWCLQGNNSS+SARLDLLL LIDDEHFSDQWHS+ISYSTNLDHTEVVLESMNSES
Sbjct: 661  NDVFVPWCLQGNNSSSSARLDLLLALIDDEHFSDQWHSIISYSTNLDHTEVVLESMNSES 720

Query: 969  LAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVAIAQSYAPFRIIISL 1028
            LAVLAKLL+R R KITNSD RK+THTWQ+ANLGNWH+EHLESAAVAIAQS++P R   + 
Sbjct: 721  LAVLAKLLNRVRGKITNSDARKVTHTWQRANLGNWHHEHLESAAVAIAQSHSPIRSSFTD 780

Query: 1029 MRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISR---P 1088
               SVLGGS+QNDC SFVSRDALIAIFEA+FQKLVSFLLHSPL WARNS S LISR   P
Sbjct: 781  FVCSVLGGSVQNDCSSFVSRDALIAIFEALFQKLVSFLLHSPLTWARNSCSLLISRPDYP 840

Query: 1089 ENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERR 1148
            E SFPK TSSSEVV MA+FALEVLDRC FCL +LGEENYLLPSILATIYAIDWDCSME +
Sbjct: 841  EISFPKYTSSSEVVVMANFALEVLDRCFFCLCHLGEENYLLPSILATIYAIDWDCSMEGK 900

Query: 1149 QDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFS 1208
            QDDMLD+KFKEE +A L+FGE VRALRQKITD+FW SC TH RKKYGSILIQFIRSAIFS
Sbjct: 901  QDDMLDEKFKEESKARLVFGESVRALRQKITDKFWNSCTTHHRKKYGSILIQFIRSAIFS 960

Query: 1209 EDSEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASN 1268
            EDSEEI SLC QWMLEILDQISQD  EEQYMLDQLLIK D WPFWIAP+FMAPNELAASN
Sbjct: 961  EDSEEIVSLCFQWMLEILDQISQDQFEEQYMLDQLLIKTDTWPFWIAPNFMAPNELAASN 1020

Query: 1269 MKNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSS-CLGKTTKNEVTSRAWLVA 1328
             KNVGLDIHKSGNHK +SL+S+ MSKIG+EKL + Q ENSS C+ K TKNEVTSRAWLVA
Sbjct: 1021 TKNVGLDIHKSGNHKFISLISMFMSKIGLEKLFNVQVENSSTCISKMTKNEVTSRAWLVA 1080

Query: 1329 EILCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNI 1388
            EILCTWKWPGG+ARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYS+R AQS +NI
Sbjct: 1081 EILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSSRAAQSFINI 1140

Query: 1389 WPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDCLR 1448
            WPYPVSLLEDIQEPFLRALAS LFSL++ENIWGRDKA S FELLVSRLFIGEAVNIDCLR
Sbjct: 1141 WPYPVSLLEDIQEPFLRALASLLFSLLEENIWGRDKAISQFELLVSRLFIGEAVNIDCLR 1200

Query: 1449 ILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLG 1508
            ILPLI+SYLVRPMCE N TFDDSGSC G+ SL EN  Q+  EGWLQRVLLFPSLNEWQLG
Sbjct: 1201 ILPLILSYLVRPMCERNSTFDDSGSCSGD-SLMENTFQSTIEGWLQRVLLFPSLNEWQLG 1260

Query: 1509 QDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHA 1568
            QDME WLLLV+SCYPFS ++GGLQTLK+DRNISTEEGSLLLELFRKQRK SGRSPA NHA
Sbjct: 1261 QDMEYWLLLVISCYPFSCTIGGLQTLKLDRNISTEEGSLLLELFRKQRKASGRSPAGNHA 1320

Query: 1569 PSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIVKSS 1628
            P VQMLLSELMVVSVGYCWKQF DEDWEFLL QLMS IQSAVVIMEEIAESVNDIIVKSS
Sbjct: 1321 PWVQMLLSELMVVSVGYCWKQFSDEDWEFLLFQLMSGIQSAVVIMEEIAESVNDIIVKSS 1380

Query: 1629 TAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRLDKL 1688
            T M+LNEILEKLEQSVLIS+PIPFCISRNALLSFSLF GSLGL GLKD+ESSSP + DKL
Sbjct: 1381 TTMDLNEILEKLEQSVLISNPIPFCISRNALLSFSLFDGSLGLHGLKDLESSSPQQFDKL 1440

Query: 1689 NHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKD 1748
            NH+NDRI E ILRMFFCTGISEAIACS SDKAASIISSSRLELPYFWDLIASSVTKSSKD
Sbjct: 1441 NHVNDRIVEGILRMFFCTGISEAIACSFSDKAASIISSSRLELPYFWDLIASSVTKSSKD 1500

Query: 1749 ARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAIIQENTSC 1808
            ARERA+KSIEFWGLSKGP+SSLYGILFS KPVPSLQYAAYVMLSTEPIS SAII+ENTSC
Sbjct: 1501 ARERAVKSIEFWGLSKGPISSLYGILFSPKPVPSLQYAAYVMLSTEPISNSAIIRENTSC 1560

Query: 1809 YLDYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQERVNIYLAWSLL 1868
            YLDYD TTEQGSTQVDFSSEYNVLLKEEIL MIEKLPDDVFDMELIAQERVNIYLAWSLL
Sbjct: 1561 YLDYDTTTEQGSTQVDFSSEYNVLLKEEILCMIEKLPDDVFDMELIAQERVNIYLAWSLL 1620

Query: 1869 LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKDTELPAGLSEAA 1928
            LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIP+EGMALQK+KDTE PAGLSEAA
Sbjct: 1621 LSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKRKDTEQPAGLSEAA 1680

Query: 1929 TAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSS 1988
            TAANQAITTGSLLFSVEFLWP+EP+KLA+FAGAIFGLM+RVLPAYVRGWFSDLRDRSKSS
Sbjct: 1681 TAANQAITTGSLLFSVEFLWPIEPVKLATFAGAIFGLMLRVLPAYVRGWFSDLRDRSKSS 1740

Query: 1989 VIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL 2048
             +ESFTK WCSPSLI NEL QIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL
Sbjct: 1741 ALESFTKVWCSPSLITNELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRL 1800

Query: 2049 PSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV 2108
            PSSYPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV
Sbjct: 1801 PSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGV 1860

Query: 2109 EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 2152
            EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF
Sbjct: 1861 EECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 1904

BLAST of Clc03G00220 vs. ExPASy TrEMBL
Match: A0A6J1DNK4 (E3 ubiquitin-protein ligase listerin OS=Momordica charantia OX=3673 GN=LOC111022033 PE=3 SV=1)

HSP 1 Score: 3240.3 bits (8400), Expect = 0.0e+00
Identity = 1647/1910 (86.23%), Postives = 1750/1910 (91.62%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MGRPKG+G RSKARPSSSSLAASLLPSDSAAN AGFGGF+GSYRLDSSL GDDA PFS  
Sbjct: 1    MGRPKGEGGRSKARPSSSSLAASLLPSDSAANAAGFGGFIGSYRLDSSLAGDDAAPFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD
Sbjct: 61   DSEVAQHLKRLSRKD-PITKLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VRRATHDTMTNLV+AAGR+IAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRRATHDTMTNLVIAAGRDIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDALILCT+EIFMYLEENLKLTP TLSDK VAKDELEEMHQQVISSSLLALATLIDVLV+
Sbjct: 181  VDALILCTTEIFMYLEENLKLTPGTLSDKAVAKDELEEMHQQVISSSLLALATLIDVLVA 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
             RSERS TGK SGE KHASKSR  ETAI+FAEKLFTEHKYF+DLL SKS ++RSATY+VL
Sbjct: 241  -RSERSETGKGSGETKHASKSR--ETAISFAEKLFTEHKYFIDLLNSKSPIIRSATYSVL 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            RSLVKNIPHAFKEQNMKTIAGSILGAFQE+DPSCHSS+WDTVLLFSKRLPNCW YVNVQK
Sbjct: 301  RSLVKNIPHAFKEQNMKTIAGSILGAFQEKDPSCHSSMWDTVLLFSKRLPNCWNYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ+ISYPALILFLDTVPP AV GEKFLL+FF+NLWVGRNPFHSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQQISYPALILFLDTVPPSAVAGEKFLLEFFQNLWVGRNPFHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQVTLVDVILVKLLWKDYLH 728
              ERLAFFQAFKECFLWG+RNAS        FCNGD+   FQVTLVDVILVKLLW+DYLH
Sbjct: 421  NAERLAFFQAFKECFLWGLRNAS-------RFCNGDNSPQFQVTLVDVILVKLLWEDYLH 480

Query: 729  VQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVKHDLLSVF 788
            VQCL+NQD A SEDA LN+    EI S KYPMSYLQDLRKCIVE+LSGIHLVK DLLSVF
Sbjct: 481  VQCLKNQDMALSEDASLNNKRTAEISSTKYPMSYLQDLRKCIVEVLSGIHLVKQDLLSVF 540

Query: 789  SMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTWPLVLLVGPTLANTF 848
            +MEFQK CISMF LTE+M VAS+TIE IIGFILELEQLS+DKDDTWPLVLLVGPTLANTF
Sbjct: 541  AMEFQKSCISMFQLTENMEVASKTIEQIIGFILELEQLSMDKDDTWPLVLLVGPTLANTF 600

Query: 849  PIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVEAQDVEASQFMQLF 908
            PII+SLDSSDGVRLLSAAVSVFGPRKI+  LFIHNNGMSST FSGVE QD+EA QFMQLF
Sbjct: 601  PIIKSLDSSDGVRLLSAAVSVFGPRKIIHELFIHNNGMSSTHFSGVEGQDLEARQFMQLF 660

Query: 909  NEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLESMNSES 968
            NE+FVPWCLQGNNSSASARLDLLL LIDDEH S+QWHSVISYSTNLDH   VLESMNSES
Sbjct: 661  NEIFVPWCLQGNNSSASARLDLLLALIDDEHLSEQWHSVISYSTNLDHPGNVLESMNSES 720

Query: 969  LAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVAIAQSYAPFRIIISL 1028
            LA+LAKLLDRAR KIT++D RK+T+TWQKANLGNWH+EHL+SAAVAIAQS+AP +   + 
Sbjct: 721  LAMLAKLLDRARGKITHNDSRKVTNTWQKANLGNWHHEHLDSAAVAIAQSHAPLKSSFTD 780

Query: 1029 MRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISRPENS 1088
               +VLGGS+Q+DC SFVSR+ L AI EAVFQKL SFL  SPLIWARNSSS LI+RP NS
Sbjct: 781  FLCAVLGGSVQSDCSSFVSRNGLTAILEAVFQKLASFLSQSPLIWARNSSSLLIARPGNS 840

Query: 1089 FPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERRQDD 1148
            F  STS S+ VAMAHFALEVLDRC FCL+NLGEEN+LLPSILA +YAIDWDCS+E RQDD
Sbjct: 841  FLNSTSYSDAVAMAHFALEVLDRCTFCLHNLGEENFLLPSILAALYAIDWDCSIEGRQDD 900

Query: 1149 MLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFSEDS 1208
            MLD+KF EER A LLFG+CV ALRQKITDQFWKSC TH+RKKYGSILIQFIRSAIF+ED+
Sbjct: 901  MLDEKFMEERSARLLFGKCVHALRQKITDQFWKSCGTHNRKKYGSILIQFIRSAIFNEDT 960

Query: 1209 EEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASNMKN 1268
            EE+ SL CQWMLEILDQISQDH EEQYMLDQLLIK D WP WIAP+FMAPNELAAS MKN
Sbjct: 961  EEVVSLSCQWMLEILDQISQDHSEEQYMLDQLLIKSDTWPVWIAPNFMAPNELAASTMKN 1020

Query: 1269 VGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSS-CLGKTTKNEVTSRAWLVAEIL 1328
            +GLDIHKSG+HKL+SLV++LMSKIG EKL SG+ ENSS CL K+T NEV SRAWLVAEIL
Sbjct: 1021 IGLDIHKSGDHKLISLVNMLMSKIGFEKLFSGEVENSSPCLDKSTNNEVISRAWLVAEIL 1080

Query: 1329 CTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNIWPY 1388
            CTWKWPGG ARGSFLPL CAYVKRSCSHESLL+STFNMLLDGALLY +R A+SI+NIWPY
Sbjct: 1081 CTWKWPGGSARGSFLPLLCAYVKRSCSHESLLNSTFNMLLDGALLYGSRAARSIINIWPY 1140

Query: 1389 PVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDCLRILP 1448
            PVS+LEDIQEPFLRALAS LF L++ENIWGRDKASSLFELLVSRLFIGE VNIDCLRILP
Sbjct: 1141 PVSILEDIQEPFLRALASLLFILLEENIWGRDKASSLFELLVSRLFIGEVVNIDCLRILP 1200

Query: 1449 LIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLGQDM 1508
            LIVS+LVRPMCE NFT  D GSC G+ S KEN+IQN AEGWLQRVL FPSLNEWQ GQDM
Sbjct: 1201 LIVSFLVRPMCERNFTL-DFGSCSGDGSSKENLIQNTAEGWLQRVLSFPSLNEWQAGQDM 1260

Query: 1509 EDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHAPSV 1568
            EDWLLLV+SCYPFSSSM GL TLK+DRNISTEE +LLLELF+KQRK S +SPAVNHAP V
Sbjct: 1261 EDWLLLVISCYPFSSSMAGL-TLKLDRNISTEESNLLLELFQKQRKISVKSPAVNHAPWV 1320

Query: 1569 QMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIVKSSTAM 1628
            QMLLSELMVVSVGYCWKQF+DEDWEFLL+QLMSWIQSAVVIMEEIAESV+DIIVKSST+ 
Sbjct: 1321 QMLLSELMVVSVGYCWKQFNDEDWEFLLLQLMSWIQSAVVIMEEIAESVDDIIVKSSTSR 1380

Query: 1629 NLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRLDKLNHL 1688
            NL+EILEKLEQSVLISDP+PFCISRNALLSFSLF GS GLQGLKDMES +P +LDKLNH+
Sbjct: 1381 NLDEILEKLEQSVLISDPVPFCISRNALLSFSLFDGSFGLQGLKDMESLNPLQLDKLNHV 1440

Query: 1689 NDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDARE 1748
            NDRI E ILRMFFCTGISEA+ACSC DKAASIISSSRLELPYFWDLIAS VTKSSKDARE
Sbjct: 1441 NDRIVEGILRMFFCTGISEAVACSCCDKAASIISSSRLELPYFWDLIASIVTKSSKDARE 1500

Query: 1749 RALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAIIQENTSCYLD 1808
            RALKSIEFWGLSKGPVSSLYGILFS KPVPSLQYAAYVMLSTEPISYSAII+ENT CYLD
Sbjct: 1501 RALKSIEFWGLSKGPVSSLYGILFSPKPVPSLQYAAYVMLSTEPISYSAIIRENTPCYLD 1560

Query: 1809 YDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQERVNIYLAWSLLLSH 1868
            YD TTEQGSTQVDFSSEYNV+LKEEI  MIEKLP++ FDMELIAQERVNIYLAWSLLLSH
Sbjct: 1561 YDATTEQGSTQVDFSSEYNVILKEEISCMIEKLPNNFFDMELIAQERVNIYLAWSLLLSH 1620

Query: 1869 LWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKDTELPAGLSEAATAA 1928
            LWSLPPSS +RERLVQYIQNSASS ILDCLFQHIP+EGMALQKKKDTELPAGLSEA+TAA
Sbjct: 1621 LWSLPPSSPSRERLVQYIQNSASSGILDCLFQHIPVEGMALQKKKDTELPAGLSEASTAA 1680

Query: 1929 NQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSSVIE 1988
            NQAIT GSLLFSVEFLW VEP+KLASFAGAIFGLM+RVLPAYVRGWFSDLRDRSKSS IE
Sbjct: 1681 NQAITIGSLLFSVEFLWLVEPVKLASFAGAIFGLMLRVLPAYVRGWFSDLRDRSKSSAIE 1740

Query: 1989 SFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS 2048
            SFTKAWCSPSLIANEL QIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS
Sbjct: 1741 SFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPSS 1800

Query: 2049 YPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEEC 2108
            YPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEEC
Sbjct: 1801 YPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEEC 1860

Query: 2109 PICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 2152
            PICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF
Sbjct: 1861 PICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 1897

BLAST of Clc03G00220 vs. ExPASy TrEMBL
Match: A0A6J1G9M2 (E3 ubiquitin-protein ligase listerin OS=Cucurbita moschata OX=3662 GN=LOC111452200 PE=3 SV=1)

HSP 1 Score: 3229.9 bits (8373), Expect = 0.0e+00
Identity = 1643/1911 (85.98%), Postives = 1742/1911 (91.16%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MGRPKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGSYRLDS+LTGDDA  FS  
Sbjct: 1    MGRPKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE LKQKSGKDVASIIPQWVFEYKKLLMDYNRD
Sbjct: 61   DGEVAQHLKRLSRKD-PTTKLKALASLSEFLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VR ATH+TMTNLV+AAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDALILCT+EIF+YLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS
Sbjct: 181  VDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
            VR+ERSGTGK SGE KHASKSR  ETAI+FAEKLFTEHKYFV LL+SKST+VRSAT++V+
Sbjct: 241  VRTERSGTGKGSGETKHASKSR--ETAISFAEKLFTEHKYFVHLLQSKSTIVRSATFSVV 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            +SLVKNIPHAFKEQNMKTI+GSILGAFQE+DPSCHSS+WD VLLFSKRLPNCWTYVNVQK
Sbjct: 301  KSLVKNIPHAFKEQNMKTISGSILGAFQEKDPSCHSSMWDAVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ ISYPALILFLDTVPP AVG +KFLL+FFENLWVGRNPFHSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQLISYPALILFLDTVPPSAVGEQKFLLNFFENLWVGRNPFHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQVTLVDVILVKLLWKDYLH 728
              ERLAFFQAFKECFLWG++NAS        FC+GDDLAHFQVTLVDVILV LLWKDYLH
Sbjct: 421  NAERLAFFQAFKECFLWGLQNAS-------RFCSGDDLAHFQVTLVDVILVNLLWKDYLH 480

Query: 729  -VQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVKHDLLSV 788
             VQCL+NQDRA S  APLN+    E PS KYPMSYLQDLRKCIVEILSGI+LV+HDLLSV
Sbjct: 481  DVQCLKNQDRALSGGAPLNNKRTGETPSTKYPMSYLQDLRKCIVEILSGIYLVEHDLLSV 540

Query: 789  FSMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTWPLVLLVGPTLANT 848
            F+ EFQK+CISMF LT++  VAS TIE IIGFILELEQLS+DKDD WPL +LVGPTLAN 
Sbjct: 541  FATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQLSMDKDDIWPLAILVGPTLANA 600

Query: 849  FPIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVEAQDVEASQFMQL 908
            FPIIRS +SSDGVRLLSAA+SVFGPR IV+ LFI NNG SST  SGVEAQDVEA QFMQ+
Sbjct: 601  FPIIRSHESSDGVRLLSAAISVFGPRNIVRELFICNNGKSSTHLSGVEAQDVEARQFMQI 660

Query: 909  FNEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLESMNSE 968
            FNEVFVPWCLQGNN SA ARLDLLL LIDDEHFS QW+SVISYS +LDHT VVLES+NSE
Sbjct: 661  FNEVFVPWCLQGNNCSAGARLDLLLTLIDDEHFSHQWNSVISYSFDLDHTGVVLESINSE 720

Query: 969  SLAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVAIAQSYAPFRIIIS 1028
            SLA+LAKLLDRAR KITNSD R +T+TWQKANLGNWH+EHLESAAVAIAQS APFR   +
Sbjct: 721  SLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHEHLESAAVAIAQSQAPFRSSFT 780

Query: 1029 LMRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLISRPEN 1088
                SVLGGS+Q+DC SFVSRD LIAIFEAVFQKLVSFLLHSPL WARNS S LI +P N
Sbjct: 781  EFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSFLLHSPLSWARNSCSLLIPKPGN 840

Query: 1089 SFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSMERRQD 1148
            SFP S SSS+VVA+AHF+LEVLDRC+FCLYNLGEENYLLPSILATIYAIDWDCS+E RQD
Sbjct: 841  SFPNSISSSDVVAIAHFSLEVLDRCVFCLYNLGEENYLLPSILATIYAIDWDCSIEERQD 900

Query: 1149 DMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSAIFSED 1208
            DMLDDKFKEERR  LLFG  VR LRQKI  QFWK+C+THDRKKYGSILIQFIRSAIFSED
Sbjct: 901  DMLDDKFKEERRERLLFGGRVRVLRQKIY-QFWKNCKTHDRKKYGSILIQFIRSAIFSED 960

Query: 1209 SEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELAASNMK 1268
            +EEI SLCCQWMLE+ DQISQDHLEEQYML QLLIK D WPFWIAP+FMA NELA SN K
Sbjct: 961  TEEIVSLCCQWMLEVKDQISQDHLEEQYMLYQLLIKGDTWPFWIAPNFMASNELATSNTK 1020

Query: 1269 NVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSS-CLGKTTKNEVTSRAWLVAEI 1328
            N+G DIHKSGNHKL+SL S+LMSKIG+EK  SGQ ENSS CLG+ T NEVT RAWLVAEI
Sbjct: 1021 NIGFDIHKSGNHKLISLASMLMSKIGLEKFFSGQVENSSPCLGEATNNEVTCRAWLVAEI 1080

Query: 1329 LCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSIVNIWP 1388
            LCTW WPGG+ARGSFLPLFCAYVK+SCSHESLLDSTFN+LLDGALL  +R AQSIVNIWP
Sbjct: 1081 LCTWNWPGGNARGSFLPLFCAYVKKSCSHESLLDSTFNILLDGALLCGSRAAQSIVNIWP 1140

Query: 1389 YPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNIDCLRIL 1448
            YP SLLEDIQEPFLRALAS LF ++KENIWGRDKASSLFELLV RLFIGEAVNIDCLRIL
Sbjct: 1141 YPDSLLEDIQEPFLRALASLLFCMLKENIWGRDKASSLFELLVRRLFIGEAVNIDCLRIL 1200

Query: 1449 PLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEWQLGQD 1508
            PLIVS+L+RPMCE N  FDDSGSC  E S KENIIQN  EGWLQRVLLFPSL++WQ G+D
Sbjct: 1201 PLIVSFLIRPMCERNTVFDDSGSCSVEGS-KENIIQNTIEGWLQRVLLFPSLSQWQAGED 1260

Query: 1509 MEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAVNHAPS 1568
            MEDWLLLV+SCYPFSSSMGGL TLK DRNISTEE SLLLE+FRKQR  SGRS  VNHAP 
Sbjct: 1261 MEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNISGRSSTVNHAPR 1320

Query: 1569 VQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIVKSSTA 1628
            VQMLLSELMVVSVGYCWKQF+DEDWEFLL QLMSWIQ AVV+MEEIAESVN IIV SST+
Sbjct: 1321 VQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESVNGIIVNSSTS 1380

Query: 1629 MNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRLDKLNH 1688
            MN NEILEKLEQSV ISDPIPFCISRNALLSFSLFYGS GLQGLKDME+ +P RLDKLNH
Sbjct: 1381 MNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMETLNPQRLDKLNH 1440

Query: 1689 LNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKSSKDAR 1748
            +NDRI E ILRMFFCTGISEAI CS  DKA +II+SSR+ELP+FWDLIASSVTKSSKDAR
Sbjct: 1441 VNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIASSVTKSSKDAR 1500

Query: 1749 ERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAIIQENTSCYL 1808
            ERA+KSIEFWGLSKGPVSSLYGILFS KPVPSLQYAAY MLSTEPISYSAII+ENTSCYL
Sbjct: 1501 ERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYSAIIRENTSCYL 1560

Query: 1809 DYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQERVNIYLAWSLLLS 1868
            DYD TTEQGSTQVDFSSEYNVLLKEEI+ MIEKLPDDVFDMEL+A ERVNI+LAWSLLLS
Sbjct: 1561 DYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAHERVNIFLAWSLLLS 1620

Query: 1869 HLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKDTELPAGLSEAATA 1928
            HLWSLPPSSSARERLVQYIQNSASSRILDC+FQHIP+EGMALQKKKDTELPAGLSEAATA
Sbjct: 1621 HLWSLPPSSSARERLVQYIQNSASSRILDCIFQHIPVEGMALQKKKDTELPAGLSEAATA 1680

Query: 1929 ANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFSDLRDRSKSSVI 1988
            ANQAITTGSLLF VEFLWPVEP+KLAS+AGAI+GLM+RVLPAYVRGWF DLRDRSKSSVI
Sbjct: 1681 ANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCDLRDRSKSSVI 1740

Query: 1989 ESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPS 2048
            ESFTKAWCSPSLIANEL QIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPS
Sbjct: 1741 ESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIRLPS 1800

Query: 2049 SYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEE 2108
            SYPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEE
Sbjct: 1801 SYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEGVEE 1860

Query: 2109 CPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 2152
            CPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF
Sbjct: 1861 CPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 1899

BLAST of Clc03G00220 vs. ExPASy TrEMBL
Match: A0A6J1KER5 (E3 ubiquitin-protein ligase listerin OS=Cucurbita maxima OX=3661 GN=LOC111493187 PE=3 SV=1)

HSP 1 Score: 3223.7 bits (8357), Expect = 0.0e+00
Identity = 1644/1916 (85.80%), Postives = 1746/1916 (91.13%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFS-- 308
            MGRPKGDGARSKARPSSSSLAASLLPSDSAAN AGFGGFLGSYRLDS+LTGDDA  FS  
Sbjct: 1    MGRPKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDI 60

Query: 309  ----VWLFKRVATSHYYFQQLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
                    KR++       +LKALASLSE LKQKSGKDVASIIPQWVFEYKKLLMDYNRD
Sbjct: 61   DGEVAQHLKRLSRKD-PTTKLKALASLSEFLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 428
            VR ATH+TMTNLV+AAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR
Sbjct: 121  VRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKR 180

Query: 429  VDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 488
            VDALILCT+EIF+YLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS
Sbjct: 181  VDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALATLIDVLVS 240

Query: 489  VRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKSKSTMVRSATYTVL 548
            VR+ERSGTGK SGE KHASKSR  ETAI+FAEKLFTEHKYFV LL+SKST+VRSAT++VL
Sbjct: 241  VRTERSGTGKGSGETKHASKSR--ETAISFAEKLFTEHKYFVHLLQSKSTIVRSATFSVL 300

Query: 549  RSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSKRLPNCWTYVNVQK 608
            +SLVKNIPHAFKEQNMKTI+GSILGAFQE+DPSCHSS+WD VLLFSKRLPNCWTYVNVQK
Sbjct: 301  KSLVKNIPHAFKEQNMKTISGSILGAFQEKDPSCHSSMWDAVLLFSKRLPNCWTYVNVQK 360

Query: 609  TVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFFENLWVGRNPFHSS 668
            TVLNRFWNFLRNGCFGSQ IS+PALILFLDTVPP AVG +KFLL+FFENLWVGRNPFHSS
Sbjct: 361  TVLNRFWNFLRNGCFGSQLISHPALILFLDTVPPSAVGEQKFLLNFFENLWVGRNPFHSS 420

Query: 669  RVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAHFQVTLVDVILVKLLWKDYLH 728
              ERLAFFQAFKECFLWG++NAS        FC+GDDLAHFQVTLVDVILV LLWKDYLH
Sbjct: 421  NAERLAFFQAFKECFLWGLQNAS-------RFCSGDDLAHFQVTLVDVILVNLLWKDYLH 480

Query: 729  -VQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEILSGIHLVKHDLLSV 788
             VQCL++QDRA S  APLN+    E PS KYPMSYLQDLRKCIVEILSGI+LV+HDLLSV
Sbjct: 481  DVQCLKSQDRALSGGAPLNNKRTAETPSTKYPMSYLQDLRKCIVEILSGIYLVEHDLLSV 540

Query: 789  FSMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTWPLVLLVGPTLANT 848
            F+ EFQK+CISMF LT++  VAS TIE IIGFILELEQLS+DKDD WPL LLVGPTLAN 
Sbjct: 541  FATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQLSMDKDDIWPLALLVGPTLANA 600

Query: 849  FPIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGVEAQDVEAS----- 908
            FPIIRS +SSDGVRLLSAA+SVFGPRKIV+ LFI NNGMSST  SGVEAQDVEA      
Sbjct: 601  FPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNGMSSTHLSGVEAQDVEAQDVEAR 660

Query: 909  QFMQLFNEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNLDHTEVVLE 968
            QFMQ+FNEVFVPWCLQGNNSSA +RLDLLL LIDDEHFS QW+SVISYS NLDHT VVLE
Sbjct: 661  QFMQIFNEVFVPWCLQGNNSSAGSRLDLLLALIDDEHFSHQWNSVISYSFNLDHTGVVLE 720

Query: 969  SMNSESLAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVAIAQSYAPF 1028
            S+NSESLA+LAKLLDRAR KITNSD R +T+TWQKANLGNWH+EHLESAAVAIAQS APF
Sbjct: 721  SINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHEHLESAAVAIAQSQAPF 780

Query: 1029 RIIISLMRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWARNSSSFLI 1088
            R   +    SVLGGS+Q+DC SFVSRD LIAIFEAVFQKLVSF+LHSPL WARNS S LI
Sbjct: 781  RSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSFILHSPLSWARNSCSLLI 840

Query: 1089 SRPENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIYAIDWDCSM 1148
             +P NSFP S SSS+VVAMAHF+LEVLDRC+FCLYNLGEENYLLPSILATIYAIDWDCS+
Sbjct: 841  PKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYLLPSILATIYAIDWDCSI 900

Query: 1149 ERRQDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSILIQFIRSA 1208
            E RQDDMLDDKFKEERR  LLFG  VR LRQKI  QFWK+C+TH+RKKYGSILIQFIRSA
Sbjct: 901  EERQDDMLDDKFKEERRERLLFGGRVRVLRQKIY-QFWKNCKTHNRKKYGSILIQFIRSA 960

Query: 1209 IFSEDSEEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWIAPDFMAPNELA 1268
            IFSED+EEI SLCCQWMLEI DQISQDHLEEQYMLDQLLIK D WPFWIAP+FMAPNELA
Sbjct: 961  IFSEDTEEIVSLCCQWMLEIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNFMAPNELA 1020

Query: 1269 ASNMKNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSS-CLGKTTKNEVTSRAW 1328
            ASN KN+G  IHKSGNHKL+SL S+LMSKIG+EK  SGQ E+SS CLG+ T NEVT RAW
Sbjct: 1021 ASNTKNIGFHIHKSGNHKLISLASMLMSKIGLEKFFSGQVESSSPCLGEATNNEVTCRAW 1080

Query: 1329 LVAEILCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGALLYSNRTAQSI 1388
            LVAEILCTWKWPGG+ARGSFLPLFCAYVKRSCSH SLL+STFN+LLDGALL  +R AQSI
Sbjct: 1081 LVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHVSLLNSTFNILLDGALLCGSRAAQSI 1140

Query: 1389 VNIWPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSRLFIGEAVNID 1448
            VNIWPYP +LL+DIQEPFLRALAS LFS++KENIWGRDKASSLFELLV RLFIGEAVNID
Sbjct: 1141 VNIWPYPDTLLDDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFIGEAVNID 1200

Query: 1449 CLRILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQRVLLFPSLNEW 1508
            CLRILPLIV++L+RPMCE N  FDDSGSC  E S KENIIQN  EGWLQRVLLFPSL++W
Sbjct: 1201 CLRILPLIVNFLIRPMCERNTVFDDSGSCSVEGS-KENIIQNTIEGWLQRVLLFPSLSQW 1260

Query: 1509 QLGQDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQRKTSGRSPAV 1568
            Q G+D EDWLLLV+SCYPFSSSM GL TLK DRNISTEEGSLLLE+FRKQR  SGRS  V
Sbjct: 1261 QAGEDAEDWLLLVISCYPFSSSMRGLHTLKPDRNISTEEGSLLLEIFRKQRNISGRSSTV 1320

Query: 1569 NHAPSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEEIAESVNDIIV 1628
            NHAP VQMLLSELMVVSVGYCWKQF+DEDWEFLL QLMSWIQ AVV+MEEIAESVN IIV
Sbjct: 1321 NHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESVNGIIV 1380

Query: 1629 KSSTAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQGLKDMESSSPHRL 1688
             SST+MN NEILEKLEQSV ISDPIPFCISRNALLSFSLFYGS GLQGLK+ME+ +P RL
Sbjct: 1381 NSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKNMETLNPQRL 1440

Query: 1689 DKLNHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPYFWDLIASSVTKS 1748
            DKLNH+NDRI E ILRMFFCTGISEAI CS  DKA +IISSSR+ELP+FWDLIASSVTKS
Sbjct: 1441 DKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIISSSRIELPHFWDLIASSVTKS 1500

Query: 1749 SKDARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLSTEPISYSAIIQEN 1808
            SKDARERA+KSIEFWGLSKGPVSSLYGILFS KPVPSLQYAAY MLSTEPISYSAII+EN
Sbjct: 1501 SKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYSAIIREN 1560

Query: 1809 TSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMELIAQERVNIYLAW 1868
            TSCYLDYD TTEQGSTQVDFSSEYNVLLKEEI+ MIEKLPDDVFDMEL+A ERVNI+LAW
Sbjct: 1561 TSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAHERVNIFLAW 1620

Query: 1869 SLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIEGMALQKKKDTELPAGLS 1928
            SLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIP+E MALQKKKDTELPAGLS
Sbjct: 1621 SLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVESMALQKKKDTELPAGLS 1680

Query: 1929 EAATAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVLPAYVRGWFSDLRDRS 1988
            EAATAANQAITTGSLLF VEFLWPVEP+KLAS+AGAI+GLM+RVLPAYVRGWF DLRDRS
Sbjct: 1681 EAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCDLRDRS 1740

Query: 1989 KSSVIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLV 2048
            KSSVIESFTKAWCSPSLIANEL QIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLV
Sbjct: 1741 KSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLV 1800

Query: 2049 IRLPSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEF 2108
            IRLPSSYPLRHVDV+CMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEF
Sbjct: 1801 IRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEF 1860

Query: 2109 EGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 2152
            EGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF
Sbjct: 1861 EGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 1904

BLAST of Clc03G00220 vs. TAIR 10
Match: AT5G58410.1 (HEAT/U-box domain-containing protein )

HSP 1 Score: 1652.1 bits (4277), Expect = 0.0e+00
Identity = 912/1930 (47.25%), Postives = 1271/1930 (65.85%), Query Frame = 0

Query: 249  MGRPKGDGARSKARPSSSSLAASLLPSDSAANVAGFGGFLGSYRLDSSLTGDDATPFSVW 308
            MG+PKGD ARSKARPSSSSLAASLLPS SAA V GFGG++GS R  +SL+ +D+  F + 
Sbjct: 1    MGKPKGDAARSKARPSSSSLAASLLPSGSAAAV-GFGGYVGSSRFQTSLSNEDSASF-LD 60

Query: 309  LFKRVATSHYYFQ------QLKALASLSELLKQKSGKDVASIIPQWVFEYKKLLMDYNRD 368
            L   VA             ++KALASLSEL+KQK GK++  IIPQW FEYKKL++DY+RD
Sbjct: 61   LDSEVAQHLQRLSRKDPTTKIKALASLSELVKQKQGKELLPIIPQWTFEYKKLILDYSRD 120

Query: 369  VRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQ--------- 428
            VRRATHD MTN+V  AGR+IAPHLKS+MGPWWFSQFD  SEVSQ+A  S Q         
Sbjct: 121  VRRATHDVMTNVVTGAGRDIAPHLKSIMGPWWFSQFDLASEVSQAAKSSFQVGSSFGNSV 180

Query: 429  ----AAFPAQEKRVDALILCTSEIFMYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSS 488
                AAFPAQEKR+ AL LC++EIF YLEENLKLTP  LSDK +A DELEEM+QQ+ISSS
Sbjct: 181  FLVEAAFPAQEKRLHALNLCSAEIFAYLEENLKLTPQNLSDKSLASDELEEMYQQMISSS 240

Query: 489  LLALATLIDVLVSVRSERSGTGKSSGEIKHASKSRSGETAIAFAEKLFTEHKYFVDLLKS 548
            L+ LATL+D+L+    + +G+   + E K ASK+R+   A + AEK+F+ HK F++ LKS
Sbjct: 241  LVGLATLLDILLR-EPDNTGSANINSESKLASKARA--VATSSAEKMFSSHKCFLNFLKS 300

Query: 549  KSTMVRSATYTVLRSLVKNIPHAFKEQNMKTIAGSILGAFQERDPSCHSSIWDTVLLFSK 608
            +S  +RSATY++L S +KN+P  F E +++++A ++LG F+E +P+CHSS+W+ VLLFSK
Sbjct: 301  ESPSIRSATYSLLSSFIKNVPEVFGEGDVRSLAPALLGVFRENNPTCHSSMWEAVLLFSK 360

Query: 609  RLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQEISYPALILFLDTVPPHAVGGEKFLLDFF 668
            + P  W Y+NV K+VLN  W FLRNGC+GS ++SYPALILFL+ +P  +V  +KF ++FF
Sbjct: 361  KFPQSWVYLNVHKSVLNHLWQFLRNGCYGSPQVSYPALILFLEVMPAQSVESDKFFVNFF 420

Query: 669  ENLWVGRNPFHSSRVERLAFFQAFKECFLWGIRNASSHFIQNWTFCNGDDLAH-FQVTLV 728
            +NL  GR+   SS  ++L+  +A  ECFLWG+RNAS        +C+  +  H  QV L+
Sbjct: 421  KNLLAGRSMCESSSTDQLSLLRATTECFLWGLRNAS-------RYCDVPNSIHDLQVDLI 480

Query: 729  DVILVKLLWKDYLHVQCLENQDRAFSEDAPLNDNMAEEIPSIKYPMSYLQDLRKCIVEIL 788
            D +LVK+LW D+  +              P N   + E   +   +SYLQ+L +CI+EIL
Sbjct: 481  DKVLVKILWADFTELS---------KGSIPPNQRKSAENLGMGNSVSYLQELGRCILEIL 540

Query: 789  SGIHLVKHDLLSVFSMEFQKDCISMFHLTESMGVASETIELIIGFILELEQLSVDKDDTW 848
            SGI+L++ +LLS F    Q+  ++M    + + + + ++  +I F+L LE+ SV + ++W
Sbjct: 541  SGINLLEQNLLSFFCKAVQESFLNMLQQGD-LEIVAGSMRKMIDFLLLLERYSVLEGESW 600

Query: 849  PLVLLVGPTLANTFPIIRSLDSSDGVRLLSAAVSVFGPRKIVQALFIHNNGMSSTQFSGV 908
            PL   +GP L+  FP IRS +  DGV+LLS +VSVFGPRK+V  L   ++  +ST  S  
Sbjct: 601  PLHQFMGPLLSKAFPWIRSSELLDGVKLLSVSVSVFGPRKVVPVLI--DDIETSTLLSVE 660

Query: 909  EAQDVEASQFMQLFNEVFVPWCLQGNNSSASARLDLLLELIDDEHFSDQWHSVISYSTNL 968
            + +++   + +++F E+F+PWC+ G +SS +AR DLL  L+DDE F+ QW  VISY  N 
Sbjct: 661  KEKNMSPEKLIKVFQEIFIPWCMDGYDSSTAARQDLLFSLLDDECFTQQWSDVISYVFNQ 720

Query: 969  DHTEVVLESMNSESLAVLAKLLDRARVKITNSDVRKLTHTWQKANLGNWHNEHLESAAVA 1028
             H           +LA +  LL++AR +IT     +  +    +   +WH+  +ES A++
Sbjct: 721  QH-------QGFNNLAAMKMLLEKARDEITKRSSGQELNQRIGSRPEHWHHTLIESTAIS 780

Query: 1029 IAQSYAPFRIIISLMRSSVLGGSLQNDCYSFVSRDALIAIFEAVFQKLVSFLLHSPLIWA 1088
            +  S +           SVLGGS Q+   SFVSR +L+ I+  + +KL+SF+  SPL   
Sbjct: 781  LVHSSSATTTSAVQFLCSVLGGSTQDSSISFVSRSSLVLIYRGILEKLLSFIKQSPLCSV 840

Query: 1089 RNSSSFLISRPENSFPKSTSSSEVVAMAHFALEVLDRCIFCLYNLGEENYLLPSILATIY 1148
             ++ S LI   E     S+SS +V+ +A FA EV+D   F L +L ++  LL ++L++I+
Sbjct: 841  NDTCSSLI--VEAIAFDSSSSVDVIVVAKFAAEVIDGSFFSLKSLSQDATLLTTVLSSIF 900

Query: 1149 AIDWDCSMERRQDDMLDDKFKEERRAGLLFGECVRALRQKITDQFWKSCRTHDRKKYGSI 1208
             ID +  M    D+ L +  KE+R+      + V A+  K+ +QFWKS     RK   S 
Sbjct: 901  IIDLENRMTSLVDNTLSES-KEKRKDRNFVCDYVHAVCSKMDNQFWKSINYDVRKSSAST 960

Query: 1209 LIQFIRSAIFSEDS---EEIGSLCCQWMLEILDQISQDHLEEQYMLDQLLIKDDKWPFWI 1268
            L QF+RS +  ED     E+  LC   M E+L+ +S D  +E+ +   LL++ D WP W+
Sbjct: 961  LAQFLRSVVLLEDDLQPFELTLLCASRMTEVLEYLSLDQSDEENICGLLLLESDAWPIWV 1020

Query: 1269 APDFMAPNELAASNMKNVGLDIHKSGNHKLVSLVSLLMSKIGIEKLLSGQGENSSCLGKT 1328
            +P   A   +    M     ++ KS + + VS +  L+ K+GI + + G  ++       
Sbjct: 1021 SPSSSA--SIDTHGMPVQLCELRKSKSQRYVSFIDSLIMKLGIHRFIVGHKDHG------ 1080

Query: 1329 TKNEVTSRAWLVAEILCTWKWPGGHARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDGAL 1388
                  S+AWL  EILCTW+WPGG  + SFLP   ++ K   S   LL+S F++LL+GAL
Sbjct: 1081 ----FASQAWLSVEILCTWEWPGGKVQTSFLPNLVSFCKDEPSSGGLLNSIFDILLNGAL 1140

Query: 1389 LYSNRTAQSIVNIWPYPVSLLEDIQEPFLRALASFLFSLVKENIWGRDKASSLFELLVSR 1448
            ++     + + N+W    + + D+ EPFLRAL SFL  L KE++WG ++A + F+++  +
Sbjct: 1141 VHVKDEEEGLGNMWVDFNNNIVDVVEPFLRALVSFLHILFKEDLWGEEEAMAAFKMITDK 1200

Query: 1449 LFIGEAVNIDCLRILPLIVSYLVRPMCETNFTFDDSGSCPGEASLKENIIQNAAEGWLQR 1508
            LFIGE  + +CLRI+P I+S ++ P+     T   SG    +  L   ++      WL+R
Sbjct: 1201 LFIGEETSKNCLRIIPYIMSIIISPL----RTKVKSGGSGKDTLLPLEVL---LRNWLER 1260

Query: 1509 VLLFPSLNEWQLGQDMEDWLLLVMSCYPFSSSMGGLQTLKVDRNISTEEGSLLLELFRKQ 1568
             L FP L  WQ G+D++DW  LV+SCYP S      +  ++ R++STEE +LLL+LFRKQ
Sbjct: 1261 SLSFPPLVLWQSGEDIQDWFQLVISCYPVSDK--AEEAKELQRHLSTEERTLLLDLFRKQ 1320

Query: 1569 RKTSGRSPAVNHAPSVQMLLSELMVVSVGYCWKQFDDEDWEFLLIQLMSWIQSAVVIMEE 1628
            ++  G S  V   P+VQ+LL+ L++++V YC   F+++DW+F+   L   IQSAVV+MEE
Sbjct: 1321 KQDPGASTVVTQLPAVQILLARLIMIAVSYCGNDFNEDDWDFVFSNLKRLIQSAVVVMEE 1380

Query: 1629 IAESVNDII--VKSSTAMNLNEILEKLEQSVLISDPIPFCISRNALLSFSLFYGSLGLQG 1688
             +E+VND I  V S      N+ LE L   V ISDP     ++NAL +FSL    +  + 
Sbjct: 1381 TSENVNDFISGVSSMEKEKENDTLEGLGHIVFISDP-SINSAQNALSAFSLLNALVNHKS 1440

Query: 1689 LKDMESSSPHRLDKLNHLNDRIFEAILRMFFCTGISEAIACSCSDKAASIISSSRLELPY 1748
            ++  ++      +  + + DRI E +LR+FFCTG++EAIA S S +AASI++S R++   
Sbjct: 1441 VEGEDNLKSLADETWDPVKDRILEGVLRLFFCTGLTEAIAASYSPEAASIVASFRVDHLQ 1500

Query: 1749 FWDLIASSVTKSSKDARERALKSIEFWGLSKGPVSSLYGILFSHKPVPSLQYAAYVMLST 1808
            FW+L+A  V  SS  AR+RA++++EFWGLS+G +SSLY I+FS  P+PSLQ AAY +LST
Sbjct: 1501 FWELVAHLVVDSSPRARDRAVRAVEFWGLSRGSISSLYAIMFSSNPIPSLQLAAYTVLST 1560

Query: 1809 EPISYSAIIQENTSCYLDYDITTEQGSTQVDFSSEYNVLLKEEILLMIEKLPDDVFDMEL 1868
            EPIS  AI+ +  +  L+ +   +Q S+     SE  +LL++E+  M+EKL  ++ D +L
Sbjct: 1561 EPISRLAIVAD-LNAPLNDESLNDQDSSNAGLPSEDKLLLRDEVSCMVEKLDHELLDTDL 1620

Query: 1869 IAQERVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPIE---GM 1928
             A ERV  +LAWSLLLS++ SLP  +  RERLVQYI+ +A+  ILD LFQHIP+E   G 
Sbjct: 1621 TAPERVQTFLAWSLLLSNVNSLPSLTQGRERLVQYIEKTANPLILDSLFQHIPLELYMGQ 1680

Query: 1929 ALQKKKDTELPAGLSEAATAANQAITTGSLLFSVEFLWPVEPMKLASFAGAIFGLMIRVL 1988
            +L KKKD ++P+ LS  A+AA +AI TGS L +VE LWP+E  K+AS AGAI+GLM+RVL
Sbjct: 1681 SL-KKKDGDIPSELSVVASAATRAIITGSSLSTVESLWPIETGKMASLAGAIYGLMLRVL 1740

Query: 1989 PAYVRGWFSDLRDRSKSSVIESFTKAWCSPSLIANELFQIKKAEFADENFSVVVSKSANE 2048
            PAYVR WFS++RDRS SS+IE+FT+ WCSPSLI NEL QIKKA+F DE+FSV +SK+ANE
Sbjct: 1741 PAYVREWFSEMRDRSASSLIEAFTRTWCSPSLIKNELSQIKKADFNDESFSVSISKAANE 1800

Query: 2049 VIATYTKDETGMDLVIRLPSSYPLRHVDVECMRSLGISEVKQRKWLLSMMSFVRNQNGAL 2108
            V+ATYTKDETGMDLVIRLP SYPL+ VDV C +S+GISE KQRKWL+SM  FVR+QNGAL
Sbjct: 1801 VVATYTKDETGMDLVIRLPVSYPLKPVDVNCAKSIGISEAKQRKWLMSMQMFVRHQNGAL 1860

Query: 2109 AEAIRIWKRNFDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSH 2151
            AEAIRIWKRN DKEFEGVE+CPICYSVIH  NHS+PR AC TCK+KFH ACL KWF TS+
Sbjct: 1861 AEAIRIWKRNSDKEFEGVEDCPICYSVIHIGNHSLPRRACVTCKYKFHKACLDKWFYTSN 1872

BLAST of Clc03G00220 vs. TAIR 10
Match: AT2G30270.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 110.9 bits (276), Expect = 1.3e-23
Identity = 76/200 (38.00%), Postives = 108/200 (54.00%), Query Frame = 0

Query: 17  IPVDLFLSRKHPDYLTNSSGDIVYRLSRRS----LKSSSVDKIVLLDAAASPLISIYRVN 76
           IPVDLF S+K P     SSGD+ +  S       L+ SS     LLD++  PL SI R++
Sbjct: 18  IPVDLFASKKLPGL---SSGDLGFADSSEHLVFILRKSSSSLKSLLDSSGVPLFSISRLH 77

Query: 77  SKLKASNSQKLSVFRLIFLRSYDLKVMEGLHWFQKESWQGFKGDIGE-EDLLFKVQRTLN 136
           + +                                  W+  KGD+ + +DL+  V+RT  
Sbjct: 78  NGV----------------------------------WELHKGDVEKRKDLVLTVKRTSK 137

Query: 137 KLRRTEFRVFLVSENLDDSNASLEMKGWPFQRSCTIYKGNTIVAQTSLMHKLHQIWVRRG 196
           +  +TE  V    E    S+ +L +KG PFQ+SCTIY  ++IVAQTSLM+KL QI+V R 
Sbjct: 138 RFSKTESEVSFAGE----SSENLVIKGVPFQKSCTIYSQDSIVAQTSLMYKLRQIYVGRS 176

Query: 197 RFRLTIFPGSVDPALIVTLI 212
           +FRLTIFPGS+D +L+V ++
Sbjct: 198 KFRLTIFPGSIDHSLVVAMV 176

BLAST of Clc03G00220 vs. TAIR 10
Match: AT1G67856.1 (RING/U-box superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 3.8e-04
Identity = 19/28 (67.86%), Postives = 23/28 (82.14%), Query Frame = 0

Query: 2122 TCKHKFHSACLYKWFSTSHKSTCPLCQS 2150
            +CKH FHSACL KWF  +H +TCPLC+S
Sbjct: 105  SCKHYFHSACLDKWFGNNH-TTCPLCRS 131