Clc02G25010 (gene) Watermelon (cordophanus) v2

BLAST of Clc02G25010 vs. NCBI nr
Match: KAF4374957.1 (hypothetical protein G4B88_004708, partial [Cannabis sativa])

HSP 1 Score: 1001.1 bits (2587), Expect = 5.1e-288
Identity = 464/764 (60.73%), Postives = 597/764 (78.14%), Query Frame = 0

Query: 1   MGVGSMRMEKKIH------------ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYL 60
           MGVGS  +E K              ER PH KPPFTLSQIKKAIPPHCF RSLLRSF Y+
Sbjct: 30  MGVGSRMLEPKSELKDSKNNNNSPIERAPHTKPPFTLSQIKKAIPPHCFQRSLLRSFFYV 89

Query: 61  LSDLTIICLFFSLAITYFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQ 120
             DL  + LF+ LA +YF LLP PLPY+AWP YW+FQGC L    +I HECGHHAFSDY+
Sbjct: 90  FRDLFYVTLFYYLATSYFHLLPHPLPYLAWPLYWIFQGCALFAFGLIGHECGHHAFSDYK 149

Query: 121 LLDDIIGLFFHSALLVPYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLP 180
            +DD++G   HSA+L+PYFS+KYSHRRHHSNT S+ RDE FVPK KSQ+PW+  Y NN  
Sbjct: 150 WIDDMVGFVIHSAILLPYFSFKYSHRRHHSNTGSINRDEAFVPKTKSQMPWFSKYLNNPL 209

Query: 181 GRLISLLGTLTLGWPLYLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMA 240
           GR+++L   LT+G+P YL FN  GR Y+RFA HYDPY PI++  ER+QI ISD G+    
Sbjct: 210 GRVLTLGFLLTIGFPSYLTFNLLGRQYDRFASHYDPYSPIYSNNERLQILISDVGVFITI 269

Query: 241 FVLYRIGAAKGALWVIRIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALAT 300
           FVLY++  A+G  WV+ +YG+P+V+V+G+LVL+TYLQHTHP+LPHY+SSEWDWLRGAL+T
Sbjct: 270 FVLYQLALARGLSWVMLVYGVPMVLVSGWLVLVTYLQHTHPALPHYDSSEWDWLRGALST 329

Query: 301 VDRDYGVLNTVFHNITDTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIW 360
           VDRD+GVLN++FHNI++THVVHHLF T+P+Y+A EAT+AVK +LGEYYR+D T ++KA+W
Sbjct: 330 VDRDFGVLNSIFHNISNTHVVHHLFPTIPYYNAVEATKAVKPILGEYYRLDETPIIKAVW 389

Query: 361 REAKECVYVEADEDADSDKGVSFLAMGGGE----DKEMKKQSCVGGSKLASG--KRITHA 420
           REAKEC+YVE+D+++   KGV +    GG+     +  + +S + GSK  +   +R+ H 
Sbjct: 390 REAKECLYVESDDESPLYKGVFWYKNKGGKMGAGGRMPEPKSELNGSKNKNSLIERVPHT 449

Query: 421 KPPFSITQIKKAIPPHCFQRSLYRSFSYVILDSILLYTFYYIATTYFPRLPHPLHFLAWP 480
           KPPF++++IKKAIPPHCF+RSL RSF+ V  D      FYY+AT+YF  +P P+ ++AWP
Sbjct: 450 KPPFTLSEIKKAIPPHCFKRSLIRSFACVFHDLFFASLFYYVATSYFHLIPKPISYIAWP 509

Query: 481 LYCLSQGIVFTGLWVIAHECGHHAFSNYQSLDDLVGFLLHSSFLIPYFSFKISHRRHHAN 540
           +Y + QG + TG+WVIAHECGHHAFS++Q +DD VG +LHS+ L+PYFS+K SHRRHH+N
Sbjct: 510 IYWIFQGCILTGVWVIAHECGHHAFSDHQWVDDTVGLILHSALLVPYFSWKYSHRRHHSN 569

Query: 541 TASLDRDEVFVPKPKSKIPWYFNYLTSPPARLLIIFSTLTLGWPMYLAFNSSGRFYDRFA 600
           T S+DRDEVFVPKPKS++  +  YL +PP R+L +F TLTLGWP+YLAFN SGR YDRFA
Sbjct: 570 TGSIDRDEVFVPKPKSQVSPFAKYLNNPPGRVLSLFVTLTLGWPLYLAFNVSGRPYDRFA 629

Query: 601 SHYDPNSPMFSQKERLQVQISNAGVVTIWYLLYKLTVVKGITWVIGMYLLPLMVMNAFVV 660
            HYDP  P++S +ERLQ+ IS+ G+    + LY L   KG+ WV+ +Y +PL+++N F+V
Sbjct: 630 CHYDPYGPIYSNRERLQIFISDIGIFIATFALYHLVSAKGLGWVVLVYGVPLLIVNGFLV 689

Query: 661 LISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDYGMVLNKMFHNITDTHVIHHLFPSIPH 720
           LI+ LQHTHP+LP+YDSSEWDWLRG L TVDRDYG +LN++FHNITDTHV+HHLF ++PH
Sbjct: 690 LITYLQHTHPALPHYDSSEWDWLRGALSTVDRDYG-ILNRVFHNITDTHVVHHLFSTMPH 749

Query: 721 YNAMEATRAVKEVLGEYYQFDGTPILKAAWREFRECVYVEADND 747
           YNAMEAT+AVK +LGEYY+ D TPI+KA WRE +EC+YVE D+D
Sbjct: 750 YNAMEATKAVKPILGEYYRLDDTPIVKAMWREAKECLYVEQDDD 792

BLAST of Clc02G25010 vs. NCBI nr
Match: PHT67072.1 (Omega-6 fatty acid desaturase, endoplasmic reticulum [Capsicum annuum])

HSP 1 Score: 902.1 bits (2330), Expect = 3.2e-258
Identity = 420/752 (55.85%), Postives = 552/752 (73.40%), Query Frame = 0

Query: 14  ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPL 73
           +R+P +KPPFT+  IKKAIPPHCF RSL+RSF Y++ DL+++ +F+ +A TYF LLP P 
Sbjct: 22  QRMPTSKPPFTIGDIKKAIPPHCFKRSLIRSFYYVVRDLSLVSVFYYIASTYFHLLPSPY 81

Query: 74  PYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSH 133
            Y+AW  YW+ QGC  TG+W+++HECGHHAFSDY  +DD +G   HSALLVPYFS KYSH
Sbjct: 82  CYLAWVAYWIVQGCAFTGIWMVSHECGHHAFSDYPWVDDTVGFILHSALLVPYFSLKYSH 141

Query: 134 RRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGR 193
            RHHS+T SL  D+  VPK KS++PW+  Y NN   R++ L+  L LG  LY   N +G+
Sbjct: 142 SRHHSHTNSLDGDQTHVPKIKSELPWFYKYSNNPLVRILLLVTALGLGTFLYWTINFTGK 201

Query: 194 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 253
           +Y RFACH+DPY PI+  RER+ IYISDAG++   +V YRI  A+G  WV+ IYGMPL+I
Sbjct: 202 NYGRFACHFDPYAPIYNDRERLWIYISDAGLIATTYVWYRIALAQGLAWVVCIYGMPLLI 261

Query: 254 VNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLF 313
            N  LVL+TYL HTHPS+PHY+SSEWDWLRGALA+VDRD+G+LN VFHN  DTHV+HHLF
Sbjct: 262 HNVTLVLVTYLNHTHPSVPHYDSSEWDWLRGALASVDRDFGLLNKVFHNFGDTHVLHHLF 321

Query: 314 STMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVSFLA 373
           +T+PHYHA EAT A+K +LGEYY+ D T + KA+WRE  EC+YVE        KG     
Sbjct: 322 TTIPHYHALEATNAIKPLLGEYYQFDGTPIYKALWREFSECIYVE--------KG---SR 381

Query: 374 MGGGEDKEMKKQSCVGGSKLASGKRITHAKPPFSITQIKKAIPPHCFQRSLYRSFSYVIL 433
           MGGG +  M   +     K    +R+  +KPPF+I  IKKAIPPHCF+RSL RSF YV+ 
Sbjct: 382 MGGGGN--MSNPTAKTELKQNPLQRMPTSKPPFTIGDIKKAIPPHCFKRSLIRSFYYVVR 441

Query: 434 DSILLYTFYYIATTYFPRLPHPLHFLAWPLYCLSQGIVFTGLWVIAHECGHHAFSNYQSL 493
           D  L+  FYYIA+TYF  LP P  +LAW  Y + QG  FTG+W+++HECGHHAFS+Y  +
Sbjct: 442 DLSLVSVFYYIASTYFHLLPSPYCYLAWVAYWIVQGCAFTGIWMVSHECGHHAFSDYPWV 501

Query: 494 DDLVGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPKPKSKIPWYFNYLTSPPAR 553
           DD VGF+LHS+ L+PYFS K SH RHH++T SLD D+  VPK KS++PW++ Y  +P  R
Sbjct: 502 DDTVGFILHSALLVPYFSLKYSHSRHHSHTNSLDGDQTHVPKIKSELPWFYKYSNNPLVR 561

Query: 554 LLIIFSTLTLGWPMYLAFNSSGRFYDRFASHYDPNSPMFSQKERLQVQISNAGVVTIWYL 613
           +L++ + L LG  +Y   N +G+ Y RFA H+DP +P+++ +ERL + IS+AG++   Y+
Sbjct: 562 ILLLVTALGLGTFLYWTINFTGKNYGRFACHFDPYAPIYNDRERLWIYISDAGLIATTYV 621

Query: 614 LYKLTVVKGITWVIGMYLLPLMVMNAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVD 673
            Y++ + +G+ WV+ +Y +PL++ N  +VL++ L HTHPS+P+YDSSEWDWLRG L +VD
Sbjct: 622 WYRIALAQGLAWVVCIYGMPLLIHNVTLVLVTYLNHTHPSVPHYDSSEWDWLRGALASVD 681

Query: 674 RDYGMVLNKMFHNITDTHVIHHLFPSIPHYNAMEATRAVKEVLGEYYQFDGTPILKAAWR 733
           RD+G+ LNK+FHN  DTHV+HHLF +IPHY+A+EAT A+K +LGEYYQFDGTPI KA WR
Sbjct: 682 RDFGL-LNKVFHNFGDTHVLHHLFTTIPHYHALEATNAIKPLLGEYYQFDGTPIYKALWR 741

Query: 734 EFRECVYVEADNDQDERASEASRSKGIFWFRD 766
           EF EC+YVE          E S   G+FWF++
Sbjct: 742 EFSECIYVE--------KGEGSHDNGVFWFKN 751

BLAST of Clc02G25010 vs. NCBI nr
Match: KVI12052.1 (Fatty acid desaturase, type 1, partial [Cynara cardunculus var. scolymus])

HSP 1 Score: 877.9 bits (2267), Expect = 6.5e-251
Identity = 417/755 (55.23%), Postives = 538/755 (71.26%), Query Frame = 0

Query: 11  KIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLP 70
           ++  R P +KPPFT+  IKKAIPPHCF+RSL+RS SYL  DL +  LF+ +A  Y P LP
Sbjct: 44  EVVNRAPSSKPPFTVGDIKKAIPPHCFNRSLIRSSSYLFMDLFLSSLFYYVAAVYIPRLP 103

Query: 71  KPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWK 130
             L Y+AWP YW+ QG    G+WVI HECGH AFSDY  L+D IG   H+ +L PYFSWK
Sbjct: 104 VSLSYVAWPVYWILQGSVQMGLWVIGHECGHQAFSDYPWLNDTIGYLLHTGMLAPYFSWK 163

Query: 131 YSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNA 190
           YSHRRHHSNT SL+ DE FVPK KS +       N  PGRL  L    T+GW LY+ FN 
Sbjct: 164 YSHRRHHSNTGSLEHDESFVPKKKSSLNSIARLLNTPPGRLFRLAILCTVGWLLYICFNV 223

Query: 191 SGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMP 250
           SGR Y +FA H+DP  PI+  RER QI ++D G++  ++ LY++  A+G  W+I IY  P
Sbjct: 224 SGRKYEKFANHFDPKSPIYNDRERFQILLTDIGLVVTSYGLYKLALAQGFTWLITIYFAP 283

Query: 251 LVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVH 310
           LVIV GFLV+IT+L HTH SLPHY+S+EW+WLRGAL+T+DRDYGV NTV H+ITDTHV H
Sbjct: 284 LVIVYGFLVVITWLHHTHRSLPHYDSTEWNWLRGALSTMDRDYGVFNTVLHHITDTHVAH 343

Query: 311 HLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVS 370
           HLF T+PHYHA EAT+A+K +LGEYY+ D T ++KA+WREA EC +VEADE  D  KG  
Sbjct: 344 HLFFTIPHYHAMEATKAIKPILGEYYQFDDTPIIKAMWREATECFFVEADEGEDKSKG-- 403

Query: 371 FLAMGGGEDKEMKKQSCVGGSKLASGKRITHAKPPFSITQIKKAIPPHCFQRSLYRSFSY 430
              MG G     +  S   G+++   KR   +KPPF++  IKKAIPPHCF RSL +S SY
Sbjct: 404 ---MGSGGRATERDNS---GTEVV--KRAPSSKPPFTLGDIKKAIPPHCFNRSLIKSSSY 463

Query: 431 VILDSILLYTFYYIATTYFPRLPHPLHFLAWPLYCLSQGIVFTGLWVIAHECGHHAFSNY 490
           +  D  L + FYY+A  Y   LP PL ++AWP+Y + QG V  GLWVI HECGH AFS+Y
Sbjct: 464 LFTDLFLSFFFYYVAAKYIVHLPTPLSYVAWPVYWILQGSVQMGLWVIGHECGHQAFSDY 523

Query: 491 QSLDDLVGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPKPKSKIPWYFNYLTSP 550
           Q L+D +G+ LH+  L PYFS+K SHRRHH+NTASL+ DE FVPK KS +      L +P
Sbjct: 524 QWLNDTIGYFLHTGMLAPYFSWKYSHRRHHSNTASLENDESFVPKKKSSLNSIARLLNTP 583

Query: 551 PARLLIIFSTLTLGWPMYLAFNSSGRFYDRFASHYDPNSPMFSQKERLQVQISNAGVVTI 610
           P RL  +    T+GW +Y+ FN SGR Y++FA+H+DP SP++S++ERLQ+ +++ G++  
Sbjct: 584 PGRLFRLTILCTIGWLLYICFNVSGRKYEKFANHFDPKSPIYSERERLQILLTDVGLLVA 643

Query: 611 WYLLYKLTVVKGITWVIGMYLLPLMVMNAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLV 670
            Y +YKL + +G TW++ +Y  PL+++  F+V+I+ L HTH SLP+YDS+EW+WLRG L 
Sbjct: 644 SYGVYKLALAQGFTWLVLVYFAPLVIVYGFLVVITWLHHTHRSLPHYDSTEWNWLRGALS 703

Query: 671 TVDRDYGMVLNKMFHNITDTHVIHHLFPSIPHYNAMEATRAVKEVLGEYYQFDGTPILKA 730
           T+DRDYG V N + H+ITDTHV HHLF +IPHY+AMEAT+A+K +LGEYYQFD TPI KA
Sbjct: 704 TMDRDYG-VFNTVLHHITDTHVAHHLFFTIPHYHAMEATKAIKPILGEYYQFDDTPIFKA 763

Query: 731 AWREFRECVYVEADNDQDERASEASRSKGIFWFRD 766
            WRE  EC +VEAD+  D        +KG++WF +
Sbjct: 764 MWREATECFFVEADDGDD--------NKGVYWFNN 779

BLAST of Clc02G25010 vs. NCBI nr
Match: KVI12034.1 (hypothetical protein Ccrd_009537 [Cynara cardunculus var. scolymus])

HSP 1 Score: 840.1 bits (2169), Expect = 1.5e-239
Identity = 408/750 (54.40%), Postives = 529/750 (70.53%), Query Frame = 0

Query: 1   MGVGSMRME----KKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIIC 60
           MG G   ++    K I  RVP   PPFTLS +KKAIP HCF RSL+RS  Y++ DL +  
Sbjct: 1   MGAGGRMLDSSEGKDILNRVP-VDPPFTLSDLKKAIPAHCFERSLIRSSYYVVHDLIVAY 60

Query: 61  LFFSLAITYFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGL 120
           +F+ LA TY PLLP PL ++AWP YW  Q    TG+WVI HECGHHAFS+ Q + DI+G 
Sbjct: 61  VFYFLANTYIPLLPTPLAFLAWPIYWFCQASICTGLWVIGHECGHHAFSEQQWIGDIVGF 120

Query: 121 FFHSALLVPYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLG 180
             HSAL  PYFSWKYSHR HH+NT SL  DEV++PK KS++  Y    NN PGR+ +L+ 
Sbjct: 121 ILHSALFTPYFSWKYSHRNHHANTNSLDNDEVYIPKRKSKVRNYSKLLNNPPGRVFTLVF 180

Query: 181 TLTLGWPLYLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGA 240
            LTLG+PLYL  N SG+ Y RFA H+DP  PIFT+RERIQ+ ISD G+L + + +    A
Sbjct: 181 RLTLGFPLYLLTNISGKKYGRFANHFDPMSPIFTERERIQVLISDLGLLAVFYAIKLAVA 240

Query: 241 AKGALWVIRIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVL 300
           AKGA WVI +YG+P+V V+ F VLITYL HTH SLPHY+S+EW+W+RGAL+T+DRD+G L
Sbjct: 241 AKGAAWVICMYGIPVVGVHVFFVLITYLHHTHLSLPHYDSTEWNWIRGALSTIDRDFGFL 300

Query: 301 NTVFHNITDTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVY 360
           N VFH++T THV+HHL S +PHYHAKEA +A+K VLGEYY++DRT + KA+WREAKEC+Y
Sbjct: 301 NRVFHDVTHTHVLHHLISYIPHYHAKEARDAIKPVLGEYYKIDRTPIFKAMWREAKECIY 360

Query: 361 VEADEDADSDKGVSFLAMGGGEDKEM----------KKQSCVGGSKLASG------KRIT 420
           +E D+D++      +  +   +   +           +    GG  L S       KR+ 
Sbjct: 361 IEPDQDSEHKGTYWYHKVSLPQFITLCVLVLPTICRSRNMGAGGRMLDSSEGENILKRVP 420

Query: 421 HAKPPFSITQIKKAIPPHCFQRSLYRSFSYVILDSILLYTFYYIATTYFPRLPHPLHFLA 480
              PPFS++ +KKAIP HCF+RSL RS  YV+ D I+ Y FY++A TY P LP PL +LA
Sbjct: 421 -VDPPFSLSDLKKAIPAHCFERSLIRSSYYVVHDLIVAYVFYFLANTYIPLLPAPLAYLA 480

Query: 481 WPLYCLSQGIVFTGLWVIAHECGHHAFSNYQSLDDLVGFLLHSSFLIPYFSFKISHRRHH 540
           WP+Y   Q  + TGLWVI HECGHHAFS YQ +DD VGF+LHS+   PYFS+K SHR HH
Sbjct: 481 WPIYWFCQASICTGLWVIGHECGHHAFSEYQWVDDTVGFILHSALFTPYFSWKYSHRNHH 540

Query: 541 ANTASLDRDEVFVPKPKSKIPWYFNYLTSPPARLLIIFSTLTLGWPMYLAFNSSGRFYDR 600
           ANT SLD DEV++PK KSK+  Y   L +PP R+  +   LTLG+P+YL  N SG+ Y R
Sbjct: 541 ANTNSLDNDEVYIPKRKSKVRNYSKLLNNPPGRVFTLVFRLTLGFPLYLLTNISGKKYGR 600

Query: 601 FASHYDPNSPMFSQKERLQVQISNAGVVTIWYLLYKLTVVKGITWVIGMYLLPLMVMNAF 660
           FA+H+DP SP+F+++ER+QV IS+ G++ ++Y +      KG  WVI MY +P++ ++ F
Sbjct: 601 FANHFDPMSPIFTERERIQVLISDLGLLAVFYAIKLAVAAKGAAWVICMYGIPVVGVHVF 660

Query: 661 VVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDYGMVLNKMFHNITDTHVIHHLFPSI 720
            VLI+ L HTH SLP+YDS+EW+W+RG L T+DRD+G  LN++FH++T THV+HHL   I
Sbjct: 661 FVLITYLHHTHLSLPHYDSTEWNWIRGALSTIDRDFGF-LNRVFHDVTHTHVLHHLISYI 720

Query: 721 PHYNAMEATRAVKEVLGEYYQFDGTPILKA 731
           PHY+A EA  A+K VLGEYY+ D TPI KA
Sbjct: 721 PHYHAKEARDAIKPVLGEYYKIDRTPIFKA 747

BLAST of Clc02G25010 vs. NCBI nr
Match: KVH90428.1 (Fatty acid desaturase, type 1 [Cynara cardunculus var. scolymus])

HSP 1 Score: 800.0 bits (2065), Expect = 1.7e-227
Identity = 383/768 (49.87%), Postives = 505/768 (65.76%), Query Frame = 0

Query: 9    EKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPL 68
            +K + +RVP  K PF +S +KKAIPPHCF RSL  S  YL  D+ I   F+ +A  Y PL
Sbjct: 463  DKNVLKRVPTEKTPFEISDLKKAIPPHCFKRSLTTSSYYLFRDICICYTFYQIASNYIPL 522

Query: 69   LPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFS 128
            LPKPL YIAWP YW  QG    G+W I H+ GHH+FS+YQ LDDI+G   HS+ L PYFS
Sbjct: 523  LPKPLAYIAWPVYWFCQGSSFMGIWSIGHDLGHHSFSEYQWLDDILGFIVHSSFLTPYFS 582

Query: 129  WKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAF 188
            +KYSHR HH++T S++ DEV++PK K+    Y    NN  G L      L L +P+Y  F
Sbjct: 583  FKYSHRSHHAHTNSMEYDEVWIPKRKAD-TLYSEVLNNPLGNLFMTFVRLLLSYPMYFTF 642

Query: 189  NASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYG 248
            N  GR YN F  H+ P  P+F   ER  +++SDAG+L   + LY++ A  GA W+  IYG
Sbjct: 643  NIHGRPYNGFVSHFYPQSPLFNDSERKLVWLSDAGMLVAIYALYKVAATAGATWLFCIYG 702

Query: 249  MPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHV 308
             PL+++N   +  T+L H+HPSL HY+S EWDW+RGAL+TVDR+YG+LNTVFH++T  HV
Sbjct: 703  APLLVMNAHFIFFTFLHHSHPSLAHYDSREWDWIRGALSTVDRNYGILNTVFHDVTCAHV 762

Query: 309  VHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKG 368
            VHHL ST+PHYH  EAT AVK +LG+YY+ D T +LKA WRE KEC++VE DE A+   G
Sbjct: 763  VHHLISTIPHYHTVEATNAVKPILGDYYKYDDTPILKAFWRETKECIFVEPDEGAEK-SG 822

Query: 369  VSFLAMGGGEDKEMKKQSCVGGSKLASG-------------KRITHAKPPFSITQIKKAI 428
            V +       D    +    G  K+ +G             KR+   KPPF I+ +KKAI
Sbjct: 823  VYWFR----SDFFFHRLFAFGDDKMGAGGRMNDTTTEKDVLKRVPTQKPPFEISDLKKAI 882

Query: 429  PPHCFQRSLYRSFSYVILDSILLYTFYYIATTYFPRLPHPLHFLAWPLYCLSQGIVFTGL 488
            P HCF+R L RS  Y+  D  + +TFY +A+ Y P LP PL ++AWP+Y   QG  F G+
Sbjct: 883  PQHCFKRDLPRSLYYLFGDIAICFTFYQLASNYIPLLPKPLAYIAWPIYWFCQGSSFMGI 942

Query: 489  WVIAHECGHHAFSNYQSLDDLVGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPK 548
            W I H+ GHH+FS YQ LDD +GF++HSSFL PYFSFK SHR HHA+T S++ DEV++PK
Sbjct: 943  WSIGHDLGHHSFSEYQWLDDAIGFVVHSSFLTPYFSFKYSHRSHHAHTNSMEYDEVWIPK 1002

Query: 549  PKSKIPWYFNYLTSPPARLLIIFSTLTLGWPMYLAFNSSGRFYDRFASHYDPNSPMFSQK 608
             K+   +Y   L +P   L + F  L L +PMY  FN  GR YD F SH+ P SP+F+  
Sbjct: 1003 RKAD-TFYSEILNNPLGNLFMTFVRLLLSFPMYFTFNIHGRPYDGFVSHFYPQSPLFNDS 1062

Query: 609  ERLQVQISNAGVVTIWYLLYKLTVVKGITWVIGMYLLPLMVMNAFVVLISSLQHTHPSLP 668
            ER  V +SNAG++   Y LY++    G TW+  +Y  PL+VMNA  +  + L H+HPSL 
Sbjct: 1063 ERKLVWLSNAGMLVAIYALYQIAATTGATWLFCIYGAPLLVMNAHFIFFTFLHHSHPSLA 1122

Query: 669  YYDSSEWDWLRGNLVTVDRDYGMVLNKMFHNITDTHVIHHLFPSIPHYNAMEATRAVKEV 728
            ++DS EWDWLRG + TVDRDYG +LN +FH++T  HV+HHL  +IPHY+ +EAT+AVK +
Sbjct: 1123 HFDSREWDWLRGAMSTVDRDYG-ILNHVFHDVTCAHVLHHLISTIPHYHTVEATKAVKPI 1182

Query: 729  LGEYYQFDGTPILKAAWREFRECVYVEADNDQDERASEASRSKGIFWF 764
            LG+YY++D TPILKA WRE + C+YVE D        E +   G++WF
Sbjct: 1183 LGDYYKYDDTPILKAFWRETKNCIYVEPD--------EGAEKSGVYWF 1214

BLAST of Clc02G25010 vs. ExPASy Swiss-Prot
Match: Q84UB9 (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAD2 PE=2 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 8.2e-196
Identity = 320/362 (88.40%), Postives = 336/362 (92.82%), Query Frame = 0

Query: 8   MEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFP 67
           MEK + ERVPHAKPPFTLSQIKK +PPHCFHRSLLRSFSYLLSDL  + LFF LAI YFP
Sbjct: 1   MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFSYLLSDLAFVSLFFYLAIAYFP 60

Query: 68  LLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYF 127
           LLP P PYIAWP YW+FQGC LTGVWVIAHECGHHAFSDYQL+DDI+GLFFHSALLVPYF
Sbjct: 61  LLPHPFPYIAWPLYWIFQGCSLTGVWVIAHECGHHAFSDYQLIDDIVGLFFHSALLVPYF 120

Query: 128 SWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLA 187
           SWKYSHRRHHSNT SL+RDEVFVPKPKSQIPWYL YFNN PGRLISL+GTLTLGWPLYLA
Sbjct: 121 SWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYLKYFNNPPGRLISLIGTLTLGWPLYLA 180

Query: 188 FNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIY 247
           FN SGR Y+RFACHYDPY PIFTKRE IQ+YISDAGILGMAF+LYRIG  KG+ WV+RIY
Sbjct: 181 FNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGVEKGSFWVMRIY 240

Query: 248 GMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTH 307
           G+PLVIVNGFLVLITYLQHTHP+LPHYESSEWDWLRGALATVDRDYGVLN VFHNITDTH
Sbjct: 241 GIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYGVLNRVFHNITDTH 300

Query: 308 VVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDK 367
           VVHHLFSTMPHYHAKEATEAVK VLGEYYR+DRT VLKAIWREAKECVYVE DEDADSDK
Sbjct: 301 VVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVEPDEDADSDK 360

Query: 368 GV 370
           GV
Sbjct: 361 GV 362

BLAST of Clc02G25010 vs. ExPASy Swiss-Prot
Match: Q84UC0 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAC PE=2 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 1.1e-181
Identity = 301/392 (76.79%), Postives = 343/392 (87.50%), Query Frame = 0

Query: 374 MGGGEDKEMKKQSCVGGSKLASGKRITHAKPPFSITQIKKAIPPHCFQRSLYRSFSYVIL 433
           MGGGE  E++  S    +KLA G+RITHAKPPFSI+QIKKAIPPHCFQRSLYRSFSYVI 
Sbjct: 1   MGGGEGIEVRSGS--SSTKLAFGERITHAKPPFSISQIKKAIPPHCFQRSLYRSFSYVIF 60

Query: 434 DSILLYTFYYIATTYFPRLPHPLHFLAWPLYCLSQGIVFTGLWVIAHECGHHAFSNYQSL 493
           D I   TFY+IA T F RLPHPLH+LAWPLY   QG VFTGLWVIAHECGH AFS+YQ +
Sbjct: 61  DFIFASTFYHIAATNFHRLPHPLHYLAWPLYWFCQGSVFTGLWVIAHECGHRAFSDYQLV 120

Query: 494 DDLVGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPKPKSKIPWYFNYLTSPPAR 553
           DD+VGFLLH+SFLIPYFSFKISHRRHH+NTASL+RDEVFVPKPK+K+PWYF +LT+PPAR
Sbjct: 121 DDVVGFLLHTSFLIPYFSFKISHRRHHSNTASLERDEVFVPKPKAKMPWYFKHLTNPPAR 180

Query: 554 LLIIFSTLTLGWPMYLAFNSSGRFYDRFASHYDPNSPMFSQKERLQVQISNAGVVTIWYL 613
           +LIIF TLTLGWPMYLAFN SGRFY+RF SH+DPNSP+FS+ E LQV ISNAG+V +WYL
Sbjct: 181 VLIIFITLTLGWPMYLAFNISGRFYERFTSHFDPNSPIFSENEWLQVHISNAGIVAVWYL 240

Query: 614 LYKLTVVKGITWVIGMYLLPLMVMNAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVD 673
           LYKL   KGI WVI MY++P+ +MNAFVVLI+SLQHTHPS PYYDS+EW+WLRGNLVT+D
Sbjct: 241 LYKLAAAKGIAWVIRMYVVPVTIMNAFVVLITSLQHTHPSFPYYDSTEWNWLRGNLVTLD 300

Query: 674 RDYGMVLNKMFHNITDTHVIHHLFPSIPHYNAMEATRAVKEVLGEYYQFDGTPILKAAWR 733
           RDYG +LNK+FHNITDTHV+HHLFPS+PHYNAMEATRAVK+VLGEYY FDGTPI KAAWR
Sbjct: 301 RDYG-ILNKVFHNITDTHVVHHLFPSMPHYNAMEATRAVKQVLGEYYHFDGTPIFKAAWR 360

Query: 734 EFRECVYVEADNDQDERASEASRSKGIFWFRD 766
           EFREC+YVE DND+      +S SKG+FWFR+
Sbjct: 361 EFRECIYVEPDNDE----GASSSSKGVFWFRN 385

BLAST of Clc02G25010 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.0e-166
Identity = 269/363 (74.10%), Postives = 309/363 (85.12%), Query Frame = 0

Query: 7   RMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYF 66
           ++E ++ +RVPH+KPPFTL Q+KKAIPPHCF RS+LRSFSY++ DLT+  +F+ +A  YF
Sbjct: 15  KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNYF 74

Query: 67  PLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPY 126
            LLP+PL Y+AWP YW  QGC LTGVWVIAHECGHHAFSDYQLLDDI+GL  HS LLVPY
Sbjct: 75  HLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVPY 134

Query: 127 FSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYL 186
           FSWK+SHRRHHSNTASL+RDEVFVPK KS I W+  Y NN PGRL +L  TLTLGWPLYL
Sbjct: 135 FSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLYL 194

Query: 187 AFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRI 246
           AFN SGR Y+RFACHYDPYGPI+T RER +IYISDAG+L + F LYR+ AAKG  WVI +
Sbjct: 195 AFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVICV 254

Query: 247 YGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDT 306
           YG+PL+IVN FLV+ITYLQHTHPS+PHY+SSEWDWLRGALATVDRDYG+LN VFHNITDT
Sbjct: 255 YGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDT 314

Query: 307 HVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSD 366
           HV HHLFSTMPHYHA EAT+A+K +LGEYY+ D T   KA+WREAKEC+YVEAD D D  
Sbjct: 315 HVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEAD-DGDES 374

Query: 367 KGV 370
           KGV
Sbjct: 375 KGV 376

BLAST of Clc02G25010 vs. ExPASy Swiss-Prot
Match: Q9SP61 (Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia OX=3673 GN=FADX PE=1 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 2.6e-165
Identity = 271/398 (68.09%), Postives = 327/398 (82.16%), Query Frame = 0

Query: 369 VSFLAMGGGEDKEMKKQSCVGGSKLASGKRITHAKPPFSITQIKKAIPPHCFQRSLYRSF 428
           +  L  GGG  K+M       G  L  G+RITHA+PPFSI+QIKKAIPPHCFQRSL RSF
Sbjct: 7   IGVLRNGGGPKKKMGP-----GQGLGPGERITHARPPFSISQIKKAIPPHCFQRSLRRSF 66

Query: 429 SYVILDSILLYTFYYIATTYFPRLPHP-LHFLAWPLYCLSQGIVFTGLWVIAHECGHHAF 488
           SY++ D  L+  FYY+A TYF RLPHP LH+LAWP+Y   QG V TG+W IAH+CGHHAF
Sbjct: 67  SYLLSDIALVSAFYYVADTYFHRLPHPLLHYLAWPVYWFCQGAVLTGMWGIAHDCGHHAF 126

Query: 489 SNYQSLDDLVGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPKPKSKIPWYFNYL 548
           S+YQ +DD+VGFL+HS   +PYFSFKISHRRHH+NT+S+DRDEVFVPKPK+K+PWYF YL
Sbjct: 127 SDYQLVDDVVGFLIHSLVFVPYFSFKISHRRHHSNTSSVDRDEVFVPKPKAKMPWYFKYL 186

Query: 549 TSPPARLLIIFSTLTLGWPMYLAFNSSGRFYDRFASHYDPNSPMFSQKERLQVQISNAGV 608
           T+PPAR+ IIF TLTLGWPMYL FN SGR+Y RF SH+DPNSP+FS KER+ V ISNAG+
Sbjct: 187 TNPPARVFIIFITLTLGWPMYLTFNISGRYYGRFTSHFDPNSPIFSPKERVLVHISNAGL 246

Query: 609 VTIWYLLYKLTVVKGITWVIGMYLLPLMVMNAFVVLISSLQHTHPSLPYYDSSEWDWLRG 668
           V   YLLY++ + KG+ W+I +Y +PL+V+NA VVLI++LQHTHPS PYYDS+EWDWLRG
Sbjct: 247 VATGYLLYRIAMAKGVGWLIRLYGVPLIVLNACVVLITALQHTHPSFPYYDSTEWDWLRG 306

Query: 669 NLVTVDRDYGMVLNKMFHNITDTHVIHHLFPSIPHYNAMEATRAVKEVLGEYYQFDGTPI 728
           NLVTVDRDYG ++N++FH+ITDTHV+HHLFPS+PHYN  EAT A K +LGEYYQFDGTPI
Sbjct: 307 NLVTVDRDYGPIMNRVFHHITDTHVVHHLFPSMPHYNGKEATVAAKRILGEYYQFDGTPI 366

Query: 729 LKAAWREFRECVYVEADNDQDERASEASRSKGIFWFRD 766
            KAAWREFRECVYVE   D+D+ A+  S SKG+FW+ +
Sbjct: 367 WKAAWREFRECVYVEP--DEDDGATSGSSSKGVFWYHN 397

BLAST of Clc02G25010 vs. ExPASy Swiss-Prot
Match: Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)

HSP 1 Score: 569.7 bits (1467), Expect = 5.0e-161
Identity = 260/368 (70.65%), Postives = 308/368 (83.70%), Query Frame = 0

Query: 2   GVGSMRMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSL 61
           G G  + +K + +RVP AKPPFTLS+IKKAIPPHCF RSLL+SFSY+L DLT++ +F+ +
Sbjct: 15  GEGDEKSQKPV-QRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSYVLYDLTLVAIFYYV 74

Query: 62  AITYFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSA 121
           A TY   LP PL Y AWP YW  QGC LTGVWVIAHECGHHAFSDYQ +DD +GL  HSA
Sbjct: 75  ATTYIDALPGPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDYQWVDDCVGLVLHSA 134

Query: 122 LLVPYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLG 181
           LLVPYFSWKYSHRRHHSNT SL+RDEVFVPKPKS++PW+  Y NN PGR+++L+ TLTLG
Sbjct: 135 LLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNPPGRVMTLIVTLTLG 194

Query: 182 WPLYLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGAL 241
           WPLYLA N SG  Y+RFACH+DPYGPI+T RER+QIYISD GI+   + LY+I AA+G  
Sbjct: 195 WPLYLALNVSGWPYDRFACHFDPYGPIYTDRERLQIYISDVGIMAATYTLYKIAAARGLA 254

Query: 242 WVIRIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFH 301
           W++ +YG+PL+IVN FLV ITYLQHTHP+LPHY+SSEWDWLRGALAT DRDYG+LN VFH
Sbjct: 255 WLVCVYGVPLLIVNAFLVTITYLQHTHPALPHYDSSEWDWLRGALATADRDYGILNKVFH 314

Query: 302 NITDTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADE 361
           NITDTHV HHLFSTMPHYHA EAT+A+K +LG+YY+ D T V KA+WREA+EC+YVE D+
Sbjct: 315 NITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAMWREARECLYVEPDD 374

Query: 362 DADSDKGV 370
            A+S KGV
Sbjct: 375 GANS-KGV 380

BLAST of Clc02G25010 vs. ExPASy TrEMBL
Match: A0A7J6FWL1 (Uncharacterized protein (Fragment) OS=Cannabis sativa OX=3483 GN=G4B88_004708 PE=3 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 2.5e-288
Identity = 464/764 (60.73%), Postives = 597/764 (78.14%), Query Frame = 0

Query: 1   MGVGSMRMEKKIH------------ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYL 60
           MGVGS  +E K              ER PH KPPFTLSQIKKAIPPHCF RSLLRSF Y+
Sbjct: 30  MGVGSRMLEPKSELKDSKNNNNSPIERAPHTKPPFTLSQIKKAIPPHCFQRSLLRSFFYV 89

Query: 61  LSDLTIICLFFSLAITYFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQ 120
             DL  + LF+ LA +YF LLP PLPY+AWP YW+FQGC L    +I HECGHHAFSDY+
Sbjct: 90  FRDLFYVTLFYYLATSYFHLLPHPLPYLAWPLYWIFQGCALFAFGLIGHECGHHAFSDYK 149

Query: 121 LLDDIIGLFFHSALLVPYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLP 180
            +DD++G   HSA+L+PYFS+KYSHRRHHSNT S+ RDE FVPK KSQ+PW+  Y NN  
Sbjct: 150 WIDDMVGFVIHSAILLPYFSFKYSHRRHHSNTGSINRDEAFVPKTKSQMPWFSKYLNNPL 209

Query: 181 GRLISLLGTLTLGWPLYLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMA 240
           GR+++L   LT+G+P YL FN  GR Y+RFA HYDPY PI++  ER+QI ISD G+    
Sbjct: 210 GRVLTLGFLLTIGFPSYLTFNLLGRQYDRFASHYDPYSPIYSNNERLQILISDVGVFITI 269

Query: 241 FVLYRIGAAKGALWVIRIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALAT 300
           FVLY++  A+G  WV+ +YG+P+V+V+G+LVL+TYLQHTHP+LPHY+SSEWDWLRGAL+T
Sbjct: 270 FVLYQLALARGLSWVMLVYGVPMVLVSGWLVLVTYLQHTHPALPHYDSSEWDWLRGALST 329

Query: 301 VDRDYGVLNTVFHNITDTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIW 360
           VDRD+GVLN++FHNI++THVVHHLF T+P+Y+A EAT+AVK +LGEYYR+D T ++KA+W
Sbjct: 330 VDRDFGVLNSIFHNISNTHVVHHLFPTIPYYNAVEATKAVKPILGEYYRLDETPIIKAVW 389

Query: 361 REAKECVYVEADEDADSDKGVSFLAMGGGE----DKEMKKQSCVGGSKLASG--KRITHA 420
           REAKEC+YVE+D+++   KGV +    GG+     +  + +S + GSK  +   +R+ H 
Sbjct: 390 REAKECLYVESDDESPLYKGVFWYKNKGGKMGAGGRMPEPKSELNGSKNKNSLIERVPHT 449

Query: 421 KPPFSITQIKKAIPPHCFQRSLYRSFSYVILDSILLYTFYYIATTYFPRLPHPLHFLAWP 480
           KPPF++++IKKAIPPHCF+RSL RSF+ V  D      FYY+AT+YF  +P P+ ++AWP
Sbjct: 450 KPPFTLSEIKKAIPPHCFKRSLIRSFACVFHDLFFASLFYYVATSYFHLIPKPISYIAWP 509

Query: 481 LYCLSQGIVFTGLWVIAHECGHHAFSNYQSLDDLVGFLLHSSFLIPYFSFKISHRRHHAN 540
           +Y + QG + TG+WVIAHECGHHAFS++Q +DD VG +LHS+ L+PYFS+K SHRRHH+N
Sbjct: 510 IYWIFQGCILTGVWVIAHECGHHAFSDHQWVDDTVGLILHSALLVPYFSWKYSHRRHHSN 569

Query: 541 TASLDRDEVFVPKPKSKIPWYFNYLTSPPARLLIIFSTLTLGWPMYLAFNSSGRFYDRFA 600
           T S+DRDEVFVPKPKS++  +  YL +PP R+L +F TLTLGWP+YLAFN SGR YDRFA
Sbjct: 570 TGSIDRDEVFVPKPKSQVSPFAKYLNNPPGRVLSLFVTLTLGWPLYLAFNVSGRPYDRFA 629

Query: 601 SHYDPNSPMFSQKERLQVQISNAGVVTIWYLLYKLTVVKGITWVIGMYLLPLMVMNAFVV 660
            HYDP  P++S +ERLQ+ IS+ G+    + LY L   KG+ WV+ +Y +PL+++N F+V
Sbjct: 630 CHYDPYGPIYSNRERLQIFISDIGIFIATFALYHLVSAKGLGWVVLVYGVPLLIVNGFLV 689

Query: 661 LISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDYGMVLNKMFHNITDTHVIHHLFPSIPH 720
           LI+ LQHTHP+LP+YDSSEWDWLRG L TVDRDYG +LN++FHNITDTHV+HHLF ++PH
Sbjct: 690 LITYLQHTHPALPHYDSSEWDWLRGALSTVDRDYG-ILNRVFHNITDTHVVHHLFSTMPH 749

Query: 721 YNAMEATRAVKEVLGEYYQFDGTPILKAAWREFRECVYVEADND 747
           YNAMEAT+AVK +LGEYY+ D TPI+KA WRE +EC+YVE D+D
Sbjct: 750 YNAMEATKAVKPILGEYYRLDDTPIVKAMWREAKECLYVEQDDD 792

BLAST of Clc02G25010 vs. ExPASy TrEMBL
Match: M1C7A7 (Microsomal omega-6-desaturase OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 3.1e-275
Identity = 455/753 (60.42%), Postives = 570/753 (75.70%), Query Frame = 0

Query: 16  VPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPLPY 75
           V  +KPPFT+  IKK IPPHCF RSL+RSFSYL+ D  I+ +F+ +A TYF  L  P  Y
Sbjct: 13  VSFSKPPFTIGDIKKVIPPHCFQRSLVRSFSYLVQDFIIVSVFYYIATTYFHPLTSPCCY 72

Query: 76  IAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSHRR 135
           +AWP YW+ QGC LTG+W+I HE GH AFSDY  ++D IGL  HSALL PYFSWKY+HRR
Sbjct: 73  LAWPIYWIAQGCVLTGIWMIGHELGHQAFSDYPWINDTIGLILHSALLTPYFSWKYTHRR 132

Query: 136 HHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLP-GRLISLLGTLTLGWPLYLAFNASGRS 195
           HHSNT+SL+ DEV+VP+ KSQ+ W   Y N  P GR+  L  TL LGWPLYL FNASGR 
Sbjct: 133 HHSNTSSLEYDEVYVPRLKSQVTWLTKYLNKNPLGRVFGLATTLNLGWPLYLTFNASGRP 192

Query: 196 YNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVIV 255
           Y RFA HY P+GPI+  RER+QIY SDAG++   +VLYRI  A+G  W++ IYG+PL I+
Sbjct: 193 YPRFASHYHPHGPIYYDRERLQIYTSDAGVIATTYVLYRIALAQGLTWLVCIYGVPLQIM 252

Query: 256 NGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLFS 315
           N F+VLIT L HTH S+PHY+ SEWDWLRGALATVDRD+GVLN VFHNITDTHV+HHLFS
Sbjct: 253 NIFIVLITLLNHTHSSVPHYDLSEWDWLRGALATVDRDFGVLNKVFHNITDTHVLHHLFS 312

Query: 316 TMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADE-DADSDKGVSFLA 375
           T+PHYH  EAT A+K +LGEYY+ D T   KA+WR+ KEC+YVE DE   D + G+    
Sbjct: 313 TIPHYHGLEATRAIKPLLGEYYQFDSTPFYKALWRDYKECIYVEKDERSQDRESGLKTKM 372

Query: 376 MGGGEDKEMKKQSCVGGSKLASGKRITHAKPPFSITQIKKAIPPHCFQRSLYRSFSYVIL 435
            GGG    M   +     K    +R+  +KPPF++  IKKAIPPHCFQRSL RS SY++ 
Sbjct: 373 GGGG---NMFAPTAKTEQKKNPLERVPSSKPPFTVGDIKKAIPPHCFQRSLIRSISYLVQ 432

Query: 436 DSILLYTFYYIATTYFPRLPHPLHFLAWPLYCLSQGIVFTGLWVIAHECGHHAFSNYQSL 495
           D +L+  FYYIA++YF  LP P ++LAWP+Y + QG V TG+WVIAHECGH AFS+YQ +
Sbjct: 433 DLMLVSVFYYIASSYFHLLPSPYYYLAWPIYWIFQGCVCTGIWVIAHECGHQAFSDYQWI 492

Query: 496 DDLVGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPKPKSKIPWYFN-YLTSPPA 555
           +D VGF+ HS+ L PYFS+K SHRRHH+NT SL+ DE  VPK K+K+ WY   Y+ +P  
Sbjct: 493 NDTVGFIFHSALLTPYFSWKYSHRRHHSNTNSLEHDENHVPKFKTKLRWYTKLYMNNPLG 552

Query: 556 RLLIIFSTLTLGWPMYLAFNSSGRFYDRFASHYDPNSPMFSQKERLQVQISNAGVVTIWY 615
           RLLI+  TLT G P+Y A N +GR YDRFASHYDP SP+++ +ERLQ+ IS+AG++   Y
Sbjct: 553 RLLILAFTLTAGLPLYYAINIAGRPYDRFASHYDPYSPIYNDRERLQIYISDAGLIATIY 612

Query: 616 LLYKLTVVKGITWVIGMYLLPLMVMNAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTV 675
           +LY++T+ +G+TWV+ +Y +PL+++N F+VLI+ L HTH SLP+YDSSEWDWLRG L TV
Sbjct: 613 VLYRITLTQGLTWVVCIYGVPLLIVNGFIVLITLLHHTHASLPHYDSSEWDWLRGALATV 672

Query: 676 DRDYGMVLNKMFHNITDTHVIHHLFPSIPHYNAMEATRAVKEVLGEYYQFDGTPILKAAW 735
           DRDYG VL K+FHNITDTHV+HHLF S+PHY+AMEAT+AV+ +LG YYQFDGTP  KA W
Sbjct: 673 DRDYG-VLTKVFHNITDTHVVHHLFSSMPHYHAMEATKAVRPLLGAYYQFDGTPFYKAIW 732

Query: 736 REFRECVYVEADNDQDERASEASRSKGIFWFRD 766
           R+F EC+YV+ DN       EAS+ KGIFW+++
Sbjct: 733 RDF-ECIYVDKDN-------EASQGKGIFWYKN 753

BLAST of Clc02G25010 vs. ExPASy TrEMBL
Match: A0A2G2YBD4 (Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Capsicum annuum OX=4072 GN=T459_31497 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.6e-258
Identity = 420/752 (55.85%), Postives = 552/752 (73.40%), Query Frame = 0

Query: 14  ERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPL 73
           +R+P +KPPFT+  IKKAIPPHCF RSL+RSF Y++ DL+++ +F+ +A TYF LLP P 
Sbjct: 22  QRMPTSKPPFTIGDIKKAIPPHCFKRSLIRSFYYVVRDLSLVSVFYYIASTYFHLLPSPY 81

Query: 74  PYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSH 133
            Y+AW  YW+ QGC  TG+W+++HECGHHAFSDY  +DD +G   HSALLVPYFS KYSH
Sbjct: 82  CYLAWVAYWIVQGCAFTGIWMVSHECGHHAFSDYPWVDDTVGFILHSALLVPYFSLKYSH 141

Query: 134 RRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNASGR 193
            RHHS+T SL  D+  VPK KS++PW+  Y NN   R++ L+  L LG  LY   N +G+
Sbjct: 142 SRHHSHTNSLDGDQTHVPKIKSELPWFYKYSNNPLVRILLLVTALGLGTFLYWTINFTGK 201

Query: 194 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 253
           +Y RFACH+DPY PI+  RER+ IYISDAG++   +V YRI  A+G  WV+ IYGMPL+I
Sbjct: 202 NYGRFACHFDPYAPIYNDRERLWIYISDAGLIATTYVWYRIALAQGLAWVVCIYGMPLLI 261

Query: 254 VNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHHLF 313
            N  LVL+TYL HTHPS+PHY+SSEWDWLRGALA+VDRD+G+LN VFHN  DTHV+HHLF
Sbjct: 262 HNVTLVLVTYLNHTHPSVPHYDSSEWDWLRGALASVDRDFGLLNKVFHNFGDTHVLHHLF 321

Query: 314 STMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVSFLA 373
           +T+PHYHA EAT A+K +LGEYY+ D T + KA+WRE  EC+YVE        KG     
Sbjct: 322 TTIPHYHALEATNAIKPLLGEYYQFDGTPIYKALWREFSECIYVE--------KG---SR 381

Query: 374 MGGGEDKEMKKQSCVGGSKLASGKRITHAKPPFSITQIKKAIPPHCFQRSLYRSFSYVIL 433
           MGGG +  M   +     K    +R+  +KPPF+I  IKKAIPPHCF+RSL RSF YV+ 
Sbjct: 382 MGGGGN--MSNPTAKTELKQNPLQRMPTSKPPFTIGDIKKAIPPHCFKRSLIRSFYYVVR 441

Query: 434 DSILLYTFYYIATTYFPRLPHPLHFLAWPLYCLSQGIVFTGLWVIAHECGHHAFSNYQSL 493
           D  L+  FYYIA+TYF  LP P  +LAW  Y + QG  FTG+W+++HECGHHAFS+Y  +
Sbjct: 442 DLSLVSVFYYIASTYFHLLPSPYCYLAWVAYWIVQGCAFTGIWMVSHECGHHAFSDYPWV 501

Query: 494 DDLVGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPKPKSKIPWYFNYLTSPPAR 553
           DD VGF+LHS+ L+PYFS K SH RHH++T SLD D+  VPK KS++PW++ Y  +P  R
Sbjct: 502 DDTVGFILHSALLVPYFSLKYSHSRHHSHTNSLDGDQTHVPKIKSELPWFYKYSNNPLVR 561

Query: 554 LLIIFSTLTLGWPMYLAFNSSGRFYDRFASHYDPNSPMFSQKERLQVQISNAGVVTIWYL 613
           +L++ + L LG  +Y   N +G+ Y RFA H+DP +P+++ +ERL + IS+AG++   Y+
Sbjct: 562 ILLLVTALGLGTFLYWTINFTGKNYGRFACHFDPYAPIYNDRERLWIYISDAGLIATTYV 621

Query: 614 LYKLTVVKGITWVIGMYLLPLMVMNAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVD 673
            Y++ + +G+ WV+ +Y +PL++ N  +VL++ L HTHPS+P+YDSSEWDWLRG L +VD
Sbjct: 622 WYRIALAQGLAWVVCIYGMPLLIHNVTLVLVTYLNHTHPSVPHYDSSEWDWLRGALASVD 681

Query: 674 RDYGMVLNKMFHNITDTHVIHHLFPSIPHYNAMEATRAVKEVLGEYYQFDGTPILKAAWR 733
           RD+G+ LNK+FHN  DTHV+HHLF +IPHY+A+EAT A+K +LGEYYQFDGTPI KA WR
Sbjct: 682 RDFGL-LNKVFHNFGDTHVLHHLFTTIPHYHALEATNAIKPLLGEYYQFDGTPIYKALWR 741

Query: 734 EFRECVYVEADNDQDERASEASRSKGIFWFRD 766
           EF EC+YVE          E S   G+FWF++
Sbjct: 742 EFSECIYVE--------KGEGSHDNGVFWFKN 751

BLAST of Clc02G25010 vs. ExPASy TrEMBL
Match: A0A103YN26 (Fatty acid desaturase, type 1 (Fragment) OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_009529 PE=3 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 3.2e-251
Identity = 417/755 (55.23%), Postives = 538/755 (71.26%), Query Frame = 0

Query: 11  KIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLP 70
           ++  R P +KPPFT+  IKKAIPPHCF+RSL+RS SYL  DL +  LF+ +A  Y P LP
Sbjct: 44  EVVNRAPSSKPPFTVGDIKKAIPPHCFNRSLIRSSSYLFMDLFLSSLFYYVAAVYIPRLP 103

Query: 71  KPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWK 130
             L Y+AWP YW+ QG    G+WVI HECGH AFSDY  L+D IG   H+ +L PYFSWK
Sbjct: 104 VSLSYVAWPVYWILQGSVQMGLWVIGHECGHQAFSDYPWLNDTIGYLLHTGMLAPYFSWK 163

Query: 131 YSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPLYLAFNA 190
           YSHRRHHSNT SL+ DE FVPK KS +       N  PGRL  L    T+GW LY+ FN 
Sbjct: 164 YSHRRHHSNTGSLEHDESFVPKKKSSLNSIARLLNTPPGRLFRLAILCTVGWLLYICFNV 223

Query: 191 SGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMP 250
           SGR Y +FA H+DP  PI+  RER QI ++D G++  ++ LY++  A+G  W+I IY  P
Sbjct: 224 SGRKYEKFANHFDPKSPIYNDRERFQILLTDIGLVVTSYGLYKLALAQGFTWLITIYFAP 283

Query: 251 LVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVH 310
           LVIV GFLV+IT+L HTH SLPHY+S+EW+WLRGAL+T+DRDYGV NTV H+ITDTHV H
Sbjct: 284 LVIVYGFLVVITWLHHTHRSLPHYDSTEWNWLRGALSTMDRDYGVFNTVLHHITDTHVAH 343

Query: 311 HLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDADSDKGVS 370
           HLF T+PHYHA EAT+A+K +LGEYY+ D T ++KA+WREA EC +VEADE  D  KG  
Sbjct: 344 HLFFTIPHYHAMEATKAIKPILGEYYQFDDTPIIKAMWREATECFFVEADEGEDKSKG-- 403

Query: 371 FLAMGGGEDKEMKKQSCVGGSKLASGKRITHAKPPFSITQIKKAIPPHCFQRSLYRSFSY 430
              MG G     +  S   G+++   KR   +KPPF++  IKKAIPPHCF RSL +S SY
Sbjct: 404 ---MGSGGRATERDNS---GTEVV--KRAPSSKPPFTLGDIKKAIPPHCFNRSLIKSSSY 463

Query: 431 VILDSILLYTFYYIATTYFPRLPHPLHFLAWPLYCLSQGIVFTGLWVIAHECGHHAFSNY 490
           +  D  L + FYY+A  Y   LP PL ++AWP+Y + QG V  GLWVI HECGH AFS+Y
Sbjct: 464 LFTDLFLSFFFYYVAAKYIVHLPTPLSYVAWPVYWILQGSVQMGLWVIGHECGHQAFSDY 523

Query: 491 QSLDDLVGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPKPKSKIPWYFNYLTSP 550
           Q L+D +G+ LH+  L PYFS+K SHRRHH+NTASL+ DE FVPK KS +      L +P
Sbjct: 524 QWLNDTIGYFLHTGMLAPYFSWKYSHRRHHSNTASLENDESFVPKKKSSLNSIARLLNTP 583

Query: 551 PARLLIIFSTLTLGWPMYLAFNSSGRFYDRFASHYDPNSPMFSQKERLQVQISNAGVVTI 610
           P RL  +    T+GW +Y+ FN SGR Y++FA+H+DP SP++S++ERLQ+ +++ G++  
Sbjct: 584 PGRLFRLTILCTIGWLLYICFNVSGRKYEKFANHFDPKSPIYSERERLQILLTDVGLLVA 643

Query: 611 WYLLYKLTVVKGITWVIGMYLLPLMVMNAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLV 670
            Y +YKL + +G TW++ +Y  PL+++  F+V+I+ L HTH SLP+YDS+EW+WLRG L 
Sbjct: 644 SYGVYKLALAQGFTWLVLVYFAPLVIVYGFLVVITWLHHTHRSLPHYDSTEWNWLRGALS 703

Query: 671 TVDRDYGMVLNKMFHNITDTHVIHHLFPSIPHYNAMEATRAVKEVLGEYYQFDGTPILKA 730
           T+DRDYG V N + H+ITDTHV HHLF +IPHY+AMEAT+A+K +LGEYYQFD TPI KA
Sbjct: 704 TMDRDYG-VFNTVLHHITDTHVAHHLFFTIPHYHAMEATKAIKPILGEYYQFDDTPIFKA 763

Query: 731 AWREFRECVYVEADNDQDERASEASRSKGIFWFRD 766
            WRE  EC +VEAD+  D        +KG++WF +
Sbjct: 764 MWREATECFFVEADDGDD--------NKGVYWFNN 779

BLAST of Clc02G25010 vs. ExPASy TrEMBL
Match: A0A103YMT9 (Uncharacterized protein OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_009537 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 7.3e-240
Identity = 408/750 (54.40%), Postives = 529/750 (70.53%), Query Frame = 0

Query: 1   MGVGSMRME----KKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIIC 60
           MG G   ++    K I  RVP   PPFTLS +KKAIP HCF RSL+RS  Y++ DL +  
Sbjct: 1   MGAGGRMLDSSEGKDILNRVP-VDPPFTLSDLKKAIPAHCFERSLIRSSYYVVHDLIVAY 60

Query: 61  LFFSLAITYFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGL 120
           +F+ LA TY PLLP PL ++AWP YW  Q    TG+WVI HECGHHAFS+ Q + DI+G 
Sbjct: 61  VFYFLANTYIPLLPTPLAFLAWPIYWFCQASICTGLWVIGHECGHHAFSEQQWIGDIVGF 120

Query: 121 FFHSALLVPYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLG 180
             HSAL  PYFSWKYSHR HH+NT SL  DEV++PK KS++  Y    NN PGR+ +L+ 
Sbjct: 121 ILHSALFTPYFSWKYSHRNHHANTNSLDNDEVYIPKRKSKVRNYSKLLNNPPGRVFTLVF 180

Query: 181 TLTLGWPLYLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGA 240
            LTLG+PLYL  N SG+ Y RFA H+DP  PIFT+RERIQ+ ISD G+L + + +    A
Sbjct: 181 RLTLGFPLYLLTNISGKKYGRFANHFDPMSPIFTERERIQVLISDLGLLAVFYAIKLAVA 240

Query: 241 AKGALWVIRIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVL 300
           AKGA WVI +YG+P+V V+ F VLITYL HTH SLPHY+S+EW+W+RGAL+T+DRD+G L
Sbjct: 241 AKGAAWVICMYGIPVVGVHVFFVLITYLHHTHLSLPHYDSTEWNWIRGALSTIDRDFGFL 300

Query: 301 NTVFHNITDTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVY 360
           N VFH++T THV+HHL S +PHYHAKEA +A+K VLGEYY++DRT + KA+WREAKEC+Y
Sbjct: 301 NRVFHDVTHTHVLHHLISYIPHYHAKEARDAIKPVLGEYYKIDRTPIFKAMWREAKECIY 360

Query: 361 VEADEDADSDKGVSFLAMGGGEDKEM----------KKQSCVGGSKLASG------KRIT 420
           +E D+D++      +  +   +   +           +    GG  L S       KR+ 
Sbjct: 361 IEPDQDSEHKGTYWYHKVSLPQFITLCVLVLPTICRSRNMGAGGRMLDSSEGENILKRVP 420

Query: 421 HAKPPFSITQIKKAIPPHCFQRSLYRSFSYVILDSILLYTFYYIATTYFPRLPHPLHFLA 480
              PPFS++ +KKAIP HCF+RSL RS  YV+ D I+ Y FY++A TY P LP PL +LA
Sbjct: 421 -VDPPFSLSDLKKAIPAHCFERSLIRSSYYVVHDLIVAYVFYFLANTYIPLLPAPLAYLA 480

Query: 481 WPLYCLSQGIVFTGLWVIAHECGHHAFSNYQSLDDLVGFLLHSSFLIPYFSFKISHRRHH 540
           WP+Y   Q  + TGLWVI HECGHHAFS YQ +DD VGF+LHS+   PYFS+K SHR HH
Sbjct: 481 WPIYWFCQASICTGLWVIGHECGHHAFSEYQWVDDTVGFILHSALFTPYFSWKYSHRNHH 540

Query: 541 ANTASLDRDEVFVPKPKSKIPWYFNYLTSPPARLLIIFSTLTLGWPMYLAFNSSGRFYDR 600
           ANT SLD DEV++PK KSK+  Y   L +PP R+  +   LTLG+P+YL  N SG+ Y R
Sbjct: 541 ANTNSLDNDEVYIPKRKSKVRNYSKLLNNPPGRVFTLVFRLTLGFPLYLLTNISGKKYGR 600

Query: 601 FASHYDPNSPMFSQKERLQVQISNAGVVTIWYLLYKLTVVKGITWVIGMYLLPLMVMNAF 660
           FA+H+DP SP+F+++ER+QV IS+ G++ ++Y +      KG  WVI MY +P++ ++ F
Sbjct: 601 FANHFDPMSPIFTERERIQVLISDLGLLAVFYAIKLAVAAKGAAWVICMYGIPVVGVHVF 660

Query: 661 VVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDYGMVLNKMFHNITDTHVIHHLFPSI 720
            VLI+ L HTH SLP+YDS+EW+W+RG L T+DRD+G  LN++FH++T THV+HHL   I
Sbjct: 661 FVLITYLHHTHLSLPHYDSTEWNWIRGALSTIDRDFGF-LNRVFHDVTHTHVLHHLISYI 720

Query: 721 PHYNAMEATRAVKEVLGEYYQFDGTPILKA 731
           PHY+A EA  A+K VLGEYY+ D TPI KA
Sbjct: 721 PHYHAKEARDAIKPVLGEYYKIDRTPIFKA 747

BLAST of Clc02G25010 vs. TAIR 10
Match: AT3G12120.1 (fatty acid desaturase 2 )

HSP 1 Score: 525.8 bits (1353), Expect = 5.9e-149
Identity = 248/365 (67.95%), Postives = 288/365 (78.90%), Query Frame = 0

Query: 5   SMRMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAIT 64
           S + E    +RVP  KPPF++  +KKAIPPHCF RS+ RSFSYL+SD+ I   F+ +A  
Sbjct: 13  SKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDIIIASCFYYVATN 72

Query: 65  YFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLV 124
           YF LLP+PL Y+AWP YW  QGC LTG+WVIAHECGHHAFSDYQ LDD +GL FHS LLV
Sbjct: 73  YFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDDTVGLIFHSFLLV 132

Query: 125 PYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPL 184
           PYFSWKYSHRRHHSNT SL+RDEVFVPK KS I WY  Y NN  GR++ L     LGWPL
Sbjct: 133 PYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIMMLTVQFVLGWPL 192

Query: 185 YLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVI 244
           YLAFN SGR Y+ FACH+ P  PI+  RER+QIY+SDAGIL + F LYR  AA+G   +I
Sbjct: 193 YLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLYRYAAAQGMASMI 252

Query: 245 RIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNIT 304
            +YG+PL+IVN FLVLITYLQHTHPSLPHY+SSEWDWLRGALATVDRDYG+LN VFHNIT
Sbjct: 253 CLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGILNKVFHNIT 312

Query: 305 DTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDAD 364
           DTHV HHLFSTMPHY+A EAT+A+K +LG+YY+ D T    A++REAKEC+YVE D + D
Sbjct: 313 DTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPDREGD 372

Query: 365 SDKGV 370
             KGV
Sbjct: 373 K-KGV 376

BLAST of Clc02G25010 vs. TAIR 10
Match: AT3G12120.2 (fatty acid desaturase 2 )

HSP 1 Score: 525.8 bits (1353), Expect = 5.9e-149
Identity = 248/365 (67.95%), Postives = 288/365 (78.90%), Query Frame = 0

Query: 5   SMRMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAIT 64
           S + E    +RVP  KPPF++  +KKAIPPHCF RS+ RSFSYL+SD+ I   F+ +A  
Sbjct: 13  SKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDIIIASCFYYVATN 72

Query: 65  YFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLV 124
           YF LLP+PL Y+AWP YW  QGC LTG+WVIAHECGHHAFSDYQ LDD +GL FHS LLV
Sbjct: 73  YFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDDTVGLIFHSFLLV 132

Query: 125 PYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTLTLGWPL 184
           PYFSWKYSHRRHHSNT SL+RDEVFVPK KS I WY  Y NN  GR++ L     LGWPL
Sbjct: 133 PYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIMMLTVQFVLGWPL 192

Query: 185 YLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVI 244
           YLAFN SGR Y+ FACH+ P  PI+  RER+QIY+SDAGIL + F LYR  AA+G   +I
Sbjct: 193 YLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLYRYAAAQGMASMI 252

Query: 245 RIYGMPLVIVNGFLVLITYLQHTHPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNIT 304
            +YG+PL+IVN FLVLITYLQHTHPSLPHY+SSEWDWLRGALATVDRDYG+LN VFHNIT
Sbjct: 253 CLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGILNKVFHNIT 312

Query: 305 DTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTNVLKAIWREAKECVYVEADEDAD 364
           DTHV HHLFSTMPHY+A EAT+A+K +LG+YY+ D T    A++REAKEC+YVE D + D
Sbjct: 313 DTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPDREGD 372

Query: 365 SDKGV 370
             KGV
Sbjct: 373 K-KGV 376

BLAST of Clc02G25010 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 237.7 bits (605), Expect = 3.2e-62
Identity = 130/346 (37.57%), Postives = 187/346 (54.05%), Query Frame = 0

Query: 2   GVGSMRMEKKIHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSL 61
           G G  + E++     P A+PPF +  I+ AIP HC+ +S LRS SY++ D+  +    ++
Sbjct: 16  GAGDRKKEERFD---PSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAA-LAI 75

Query: 62  AITYFPLLPKPLPYIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSA 121
           A  Y         +  WP YW  QG     ++V+ H+CGH +FSD  LL+ ++G   HS 
Sbjct: 76  AAVYVD------SWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSF 135

Query: 122 LLVPYFSWKYSHRRHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTL--- 181
           +LVPY  W+ SHR HH N   ++ DE +VP P+         +  LP     L  T+   
Sbjct: 136 ILVPYHGWRISHRTHHQNHGHVENDESWVPLPE-------RVYKKLPHSTRMLRYTVPLP 195

Query: 182 TLGWPLYLAFNASGRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAK 241
            L +PLYL + + G+       H++PY  +F   ER  I  S      M   L  +    
Sbjct: 196 MLAYPLYLCYRSPGKE----GSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVF 255

Query: 242 GALWVIRIYGMPLVIVNGFLVLITYLQH--THPSLPHYESSEWDWLRGALATVDRDYGVL 301
           G L V+++YG+P +I   +L  +TYL H      LP Y   EW +LRG L T+DRDYG+ 
Sbjct: 256 GPLAVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIF 315

Query: 302 NTVFHNITDTHVVHHLFSTMPHYHAKEATEAVKKVLGEYYRMDRTN 343
           N + H+I  THV+HHLF  +PHYH  +AT+A K VLG YYR  +T+
Sbjct: 316 NNIHHDI-GTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKTS 339

BLAST of Clc02G25010 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 221.5 bits (563), Expect = 2.4e-57
Identity = 126/326 (38.65%), Postives = 181/326 (55.52%), Query Frame = 0

Query: 17  PHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLAITYFPLLPKPLPYI 76
           P A PPF L+ I+ AIP HC+ ++  +S SY++ D+ I+   F+LA     L      +I
Sbjct: 90  PGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAIV---FALAAGAAYL----NNWI 149

Query: 77  AWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSHRRH 136
            WP YWL QG     ++V+ H+CGH +FS+   L+ ++G   HS++LVPY  W+ SHR H
Sbjct: 150 VWPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTH 209

Query: 137 HSNTASLQRDEVFVPKPKSQIPWYLNYFNNL--PGRLISL-LGTLTLGWPLYLAFNASGR 196
           H N   ++ DE + P  +         +N L  P R     L  + L +P YL   + G+
Sbjct: 210 HQNHGHVENDESWHPMSE-------KIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGK 269

Query: 197 SYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPLVI 256
                  HY P   +F  +ER  +  S A    MA +L  +    G + ++++YG+P  I
Sbjct: 270 K----GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWI 329

Query: 257 VNGFLVLITYLQH--THPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVVHH 316
              +L  +TYL H      LP Y   EW +LRG L T+DRDYG++N + H+I  THV+HH
Sbjct: 330 NVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDI-GTHVIHH 389

Query: 317 LFSTMPHYHAKEATEAVKKVLGEYYR 338
           LF  +PHYH  EATEA K VLG+YYR
Sbjct: 390 LFPQIPHYHLVEATEAAKPVLGKYYR 396

BLAST of Clc02G25010 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 218.0 bits (554), Expect = 2.6e-56
Identity = 126/328 (38.41%), Postives = 177/328 (53.96%), Query Frame = 0

Query: 17  PHAKPPFTLSQIKKAIPPHCFHRSLLRSFSYLLSDLTIICLFFSLA--ITYFPLLPKPLP 76
           P A PPF L+ I+ AIP HC+ ++   S SY++ D+ I+   F LA    YF        
Sbjct: 83  PGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIV---FGLAAVAAYFN------N 142

Query: 77  YIAWPFYWLFQGCFLTGVWVIAHECGHHAFSDYQLLDDIIGLFFHSALLVPYFSWKYSHR 136
           ++ WP YW  QG     ++V+ H+CGH +FS+   L+ + G   HS++LVPY  W+ SHR
Sbjct: 143 WLLWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHR 202

Query: 137 RHHSNTASLQRDEVFVPKPKSQIPWYLNYFNNLPGRLISLLGTL---TLGWPLYLAFNAS 196
            HH N   ++ DE + P P+S        + NL         TL    L +P YL     
Sbjct: 203 THHQNHGHVENDESWHPLPES-------IYKNLEKTTQMFRFTLPFPMLAYPFYL----W 262

Query: 197 GRSYNRFACHYDPYGPIFTKRERIQIYISDAGILGMAFVLYRIGAAKGALWVIRIYGMPL 256
            RS  +   HY P   +F  +E+  +  S A    MA +L  +    G + ++++YG+P 
Sbjct: 263 NRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPY 322

Query: 257 VIVNGFLVLITYLQH--THPSLPHYESSEWDWLRGALATVDRDYGVLNTVFHNITDTHVV 316
            I   +L  +TYL H      LP Y   EW +LRG L T+DRDYG +N + H+I  THV+
Sbjct: 323 WIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDI-GTHVI 382

Query: 317 HHLFSTMPHYHAKEATEAVKKVLGEYYR 338
           HHLF  +PHYH  EATEA K VLG+YYR
Sbjct: 383 HHLFPQIPHYHLVEATEAAKPVLGKYYR 389