Clc02G24950 (gene) Watermelon (cordophanus) v2

BLAST of Clc02G24950 vs. NCBI nr
Match: XP_038902757.1 (DNA topoisomerase 6 subunit B isoform X1 [Benincasa hispida])

HSP 1 Score: 1295.4 bits (3351), Expect = 0.0e+00
Identity = 660/673 (98.07%), Postives = 667/673 (99.11%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           M+ GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MDAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKD WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDQWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 YAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEF+ KMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQ HASKKKR DCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQAHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFT 660
           ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDAT +FT
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATDSFT 660

Query: 661 DFQSPLFVFRLVY 674
           DF+SPLFVFRL+Y
Sbjct: 661 DFRSPLFVFRLIY 673

BLAST of Clc02G24950 vs. NCBI nr
Match: XP_008449755.1 (PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo] >KAA0041365.1 DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 658/672 (97.92%), Postives = 667/672 (99.26%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 YAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF+ KMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQVHASKKKR DCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAEPKEE+YIQSLDAT NFT
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLV 673
           DFQSPLFVFRL+
Sbjct: 661 DFQSPLFVFRLI 672

BLAST of Clc02G24950 vs. NCBI nr
Match: XP_011653575.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucumis sativus] >KAE8649491.1 hypothetical protein Csa_018197 [Cucumis sativus])

HSP 1 Score: 1287.7 bits (3331), Expect = 0.0e+00
Identity = 655/672 (97.47%), Postives = 665/672 (98.96%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGK 
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360

Query: 361 YAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF+ KMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQ QDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQAQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKR DCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFT 660
           ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAE KEE+YIQSLDAT++FT
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHSFT 660

Query: 661 DFQSPLFVFRLV 673
           DFQSPLFVFRL+
Sbjct: 661 DFQSPLFVFRLI 672

BLAST of Clc02G24950 vs. NCBI nr
Match: XP_023532908.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 650/673 (96.58%), Postives = 662/673 (98.37%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME+GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIH+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEF  KMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMA VHASKKKR D E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFT 660
           ILSKVSAR+ITNETLRDKLAQHVEQVDHEMAMEYATQSGASAE KEEIYIQSLDAT NFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLVY 674
           DF+SPLFVFRLV+
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of Clc02G24950 vs. NCBI nr
Match: TYK21651.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1270.4 bits (3286), Expect = 0.0e+00
Identity = 653/691 (94.50%), Postives = 661/691 (95.66%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDN----------- 60
           ME GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDN           
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNVSCIQLLSLSL 60

Query: 61  --------------PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120
                         PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG
Sbjct: 61  SLSPSLSLSVHFLRPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120

Query: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180
           LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA
Sbjct: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180

Query: 181 SFYRVTCKDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240
           SFYRVTCKDNG+GMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI
Sbjct: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240

Query: 241 EISSSMKGQNYISFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300
           EISSSMKGQNY SFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI
Sbjct: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300

Query: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360
           LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK
Sbjct: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360

Query: 361 RLIAETAKQNLLQFLQHEFVNIGKSYAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFR 420
           RLIAETAKQNLLQFLQHEFVNIGKS+AERLIGEMGPEF+ KMSVKSLTSQQIVRIHQLFR
Sbjct: 361 RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420

Query: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKD 480
           QAKFDDPSGDCLSPAGEYNLRLGIIKELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKD
Sbjct: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480

Query: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540
           VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF
Sbjct: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540

Query: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600
           KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA
Sbjct: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600

Query: 601 VYNVLKEMAQVHASKKKRGDCEDAEILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYA 660
           +YNVLKEMAQVHASKKKR DCEDAEILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYA
Sbjct: 601 IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA 660

Query: 661 TQSGASAEPKEEIYIQSLDATYNFTDFQSPL 667
           TQSGASAEPKEE+YIQSLDAT NFTDFQSPL
Sbjct: 661 TQSGASAEPKEEMYIQSLDATDNFTDFQSPL 691

BLAST of Clc02G24950 vs. ExPASy Swiss-Prot
Match: Q9C5V6 (DNA topoisomerase 6 subunit B OS=Arabidopsis thaliana OX=3702 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 545/662 (82.33%), Postives = 604/662 (91.24%), Query Frame = 0

Query: 12  EPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAES 71
           E KKG SK  +   ES LKQKSPAEFFAENKNIAGFDNPGK LYTTVRELVEN+LDSAES
Sbjct: 8   ETKKGSSKNSKDSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAES 67

Query: 72  ISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQA 131
           ISELP VE+TIEEI+K+KFNSMIGL+DRERVD +LYDDYET KAR KRLAKEARA EIQA
Sbjct: 68  ISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQA 127

Query: 132 KNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGRVLSGTKYGLKQ 191
           KN A GKK +EP  SK +K RGEAS+Y+VTCKDNG+GMPHDDIPNMFGRVLSGTKYGLKQ
Sbjct: 128 KNLASGKKNKEPGVSKVLKARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 187

Query: 192 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNIPHIHIQEKRDN 251
           TRGKFGLGAKMALIWSKMSTGLPIEISSSMK QNY++FCRLDIDIHRNIPHIH+ EK+ N
Sbjct: 188 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGN 247

Query: 252 KDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRR 311
           K+ WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+F+S+TP+KN+TI+F+RR
Sbjct: 248 KEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFISETPEKNVTIKFTRR 307

Query: 312 TDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKSYAERLIGEMGP 371
           TDVMPP+P+ETKHHPS+VDLLLIKRLI +T+K+ LLQFLQ+EFVNI K+ A RLIGEMGP
Sbjct: 308 TDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGP 367

Query: 372 EFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDLVATY 431
           +F   M+VKS+TSQQ+VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPD+VATY
Sbjct: 368 DFGPSMAVKSVTSQQMVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 427

Query: 432 SGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWN 491
           SGSAQVFEGHPFIVEAGVS+GG+DVKQG+NIFRFANRIPLLFEQGADVVTRTALKRINWN
Sbjct: 428 SGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWN 487

Query: 492 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVK 551
           SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIA+AVKSAIQQCCIQLKSKIVK
Sbjct: 488 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVK 547

Query: 552 KIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAEILSKVSARLIT 611
           ++QAREQQERKR+LSRYIPDAT AVY VLK+M + H +K+KR   ED  +L KVS ++IT
Sbjct: 548 RLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKRYGEEDIVMLDKVSKQIIT 607

Query: 612 NETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDAT-YNFTDFQSPLFVFR 671
            ETL++KLA+HVEQVD+EMA+EYATQSG S EP+E IY+Q LD    NF D  SP FVFR
Sbjct: 608 KETLKEKLAEHVEQVDYEMALEYATQSGVSEEPRENIYLQHLDPNKSNFIDLHSPTFVFR 667

Query: 672 LV 673
           L+
Sbjct: 668 LM 669

BLAST of Clc02G24950 vs. ExPASy Swiss-Prot
Match: B8BDQ4 (DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. indica OX=39946 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 1.8e-308
Identity = 521/648 (80.40%), Postives = 593/648 (91.51%), Query Frame = 0

Query: 25  KESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEE 84
           + SLLKQKSPAEFFAENKNIAGFDNPGK LYTT+RELVEN+LDSAESISELP +EI IEE
Sbjct: 49  ESSLLKQKSPAEFFAENKNIAGFDNPGKSLYTTMRELVENALDSAESISELPDIEIIIEE 108

Query: 85  ILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPA 144
           I K+KFN+MIGLVDR+R+DEELYDD+E+AKAREKRLAKEAR QE QAKNAALGKK +E  
Sbjct: 109 ITKSKFNTMIGLVDRQRIDEELYDDFESAKAREKRLAKEARFQETQAKNAALGKKVKEAP 168

Query: 145 ASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMAL 204
           A++G KGRGEA+F+RVTCKDNGRGMPHDDIPNM GRVLSGTKYGL+QTRGKFGLGAKMAL
Sbjct: 169 AARG-KGRGEAAFFRVTCKDNGRGMPHDDIPNMLGRVLSGTKYGLRQTRGKFGLGAKMAL 228

Query: 205 IWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVI 264
           IWSKMSTGLPIEI SSMKGQN+ISFCRLDIDIH+N+PH+H+ EKR+NKD WHGAE+QV+I
Sbjct: 229 IWSKMSTGLPIEIKSSMKGQNFISFCRLDIDIHKNVPHVHLHEKRENKDRWHGAELQVII 288

Query: 265 EGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKH 324
           EGNWTT+RSKILHYMRQMAVITPYAQFLF+F+SD+PDKN+TI+F+RRTDVMPP+PL+TKH
Sbjct: 289 EGNWTTHRSKILHYMRQMAVITPYAQFLFRFLSDSPDKNLTIQFARRTDVMPPIPLQTKH 348

Query: 325 HPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKSYAERLIGEMGPEFNSKMSVKSLTS 384
           HPSAVDLLLIKRLI+ET KQNLLQFLQHEFVNI KS+AERLIGEMGP+F++K +VKSLTS
Sbjct: 349 HPSAVDLLLIKRLISETTKQNLLQFLQHEFVNISKSHAERLIGEMGPDFSAKTTVKSLTS 408

Query: 385 QQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDLVATYSGSAQVFEGHPFI 444
           QQ+VRIHQLFRQAKFDDPSG+CLSPAGEYNLRLGIIKELHPDLVAT++ S QVFEGHPFI
Sbjct: 409 QQLVRIHQLFRQAKFDDPSGNCLSPAGEYNLRLGIIKELHPDLVATHASSPQVFEGHPFI 468

Query: 445 VEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGV 504
           VEAG+SIGGKDVK GLNIFR+ANRIPLLFEQGADV+TRTALKRINW+SYKINQ QDKIGV
Sbjct: 469 VEAGISIGGKDVKHGLNIFRYANRIPLLFEQGADVITRTALKRINWSSYKINQQQDKIGV 528

Query: 505 FVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRN 564
           FVSIVSTKIPFKGTGKEYIGDDI+EIASAV+SA++QCC+QLKSKIVKK+QARE+Q+RKRN
Sbjct: 529 FVSIVSTKIPFKGTGKEYIGDDITEIASAVQSALKQCCLQLKSKIVKKLQARERQDRKRN 588

Query: 565 LSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAEILSKVSARLITNETLRDKLAQHVE 624
           L+RYIPD   A+   L E+A     K+ R D ED E+L KV++  +T  T RD L QHVE
Sbjct: 589 LNRYIPDVARAIMETLGEIADESPPKRPRYDKEDEELLEKVNSEEVTEMTFRDCLTQHVE 648

Query: 625 QVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFTDFQSPLFVFRLV 673
           QVD+EMA+EYA QSG S EP+E +Y+ SL+ +Y F DFQSP+FVFR +
Sbjct: 649 QVDYEMALEYAMQSGVSEEPREALYLNSLEGSYKFIDFQSPVFVFRFI 695

BLAST of Clc02G24950 vs. ExPASy Swiss-Prot
Match: Q6H442 (DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 1.2e-307
Identity = 520/648 (80.25%), Postives = 592/648 (91.36%), Query Frame = 0

Query: 25  KESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEE 84
           + SLLKQKSPAEFFAENKNIAGFDNPGK LYTT+RELVEN+LDSAESISELP +EI IEE
Sbjct: 49  ESSLLKQKSPAEFFAENKNIAGFDNPGKSLYTTMRELVENALDSAESISELPDIEIIIEE 108

Query: 85  ILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPA 144
           I K+KFN+MIGLVDR+R+DEELYDD+E+AKAREKRLAKEAR QE QAKNAALGKK +E  
Sbjct: 109 ITKSKFNTMIGLVDRQRIDEELYDDFESAKAREKRLAKEARFQETQAKNAALGKKVKEAP 168

Query: 145 ASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMAL 204
           A++G KGRGEA+F+RVTCKDNGRGMPHDDIPNM GRVLSGTKYGL+QTRGKFGLGAKMAL
Sbjct: 169 AARG-KGRGEAAFFRVTCKDNGRGMPHDDIPNMLGRVLSGTKYGLRQTRGKFGLGAKMAL 228

Query: 205 IWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVI 264
           IWSKMSTGLPIEI SSMKGQN+ISFC LDIDIH+N+PH+H+ EKR+NKD WHGAE+QV+I
Sbjct: 229 IWSKMSTGLPIEIKSSMKGQNFISFCLLDIDIHKNVPHVHLHEKRENKDRWHGAELQVII 288

Query: 265 EGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKH 324
           EGNWTT+RSKILHYMRQMAVITPYAQFLF+F+SD+PDKN+TI+F+RRTDVMPP+PL+TKH
Sbjct: 289 EGNWTTHRSKILHYMRQMAVITPYAQFLFRFLSDSPDKNLTIQFARRTDVMPPIPLQTKH 348

Query: 325 HPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKSYAERLIGEMGPEFNSKMSVKSLTS 384
           HPSAVDLLLIKRLI+ET KQNLLQFLQHEFVNI KS+AERLIGEMGP+F++K +VKSLTS
Sbjct: 349 HPSAVDLLLIKRLISETTKQNLLQFLQHEFVNISKSHAERLIGEMGPDFSAKTTVKSLTS 408

Query: 385 QQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDLVATYSGSAQVFEGHPFI 444
           QQ+VRIHQLFRQAKFDDPSG+CLSPAGEYNLRLGIIKELHPDLVAT++ S QVFEGHPFI
Sbjct: 409 QQLVRIHQLFRQAKFDDPSGNCLSPAGEYNLRLGIIKELHPDLVATHASSPQVFEGHPFI 468

Query: 445 VEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGV 504
           VEAG+SIGGKDVK GLNIFR+ANRIPLLFEQGADV+TRTALKRINW+SYKINQ QDKIGV
Sbjct: 469 VEAGISIGGKDVKHGLNIFRYANRIPLLFEQGADVITRTALKRINWSSYKINQQQDKIGV 528

Query: 505 FVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRN 564
           FVSIVSTKIPFKGTGKEYIGDDI+EIASAV+SA++QCC+QLKSKIVKK+QARE+Q+RKRN
Sbjct: 529 FVSIVSTKIPFKGTGKEYIGDDITEIASAVQSALKQCCLQLKSKIVKKLQARERQDRKRN 588

Query: 565 LSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAEILSKVSARLITNETLRDKLAQHVE 624
           L+RYIPD   A+   L E+A     K+ R D ED E+L KV++  +T  T RD L QHVE
Sbjct: 589 LNRYIPDVARAIMETLGEIADESPPKRPRYDKEDEELLEKVNSEEVTEMTFRDCLTQHVE 648

Query: 625 QVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFTDFQSPLFVFRLV 673
           QVD+EMA+EYA QSG S EP+E +Y+ SL+ +Y F DFQSP+FVFR +
Sbjct: 649 QVDYEMALEYAMQSGVSEEPREALYLNSLEGSYKFIDFQSPVFVFRFI 695

BLAST of Clc02G24950 vs. ExPASy Swiss-Prot
Match: Q9YE64 (Type 2 DNA topoisomerase 6 subunit B OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=top6B PE=3 SV=2)

HSP 1 Score: 306.2 bits (783), Expect = 9.0e-82
Identity = 187/584 (32.02%), Postives = 302/584 (51.71%), Query Frame = 0

Query: 30  KQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTK 89
           ++ S AEFFA+NK +AGF NP + LY T+RELVENSLD+ ++   LP + I+I +I    
Sbjct: 29  REMSVAEFFAKNKELAGFANPTRALYQTIRELVENSLDATDAKGILPWIHISIRQI---- 88

Query: 90  FNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGM 149
                                                                    +G 
Sbjct: 89  ---------------------------------------------------------EGS 148

Query: 150 KGRGEASFYRVTCKDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKM 209
           +GRG    Y VT +DNG G+P   +   FG+VL  +KY ++QTRG +GLG K A+++ +M
Sbjct: 149 EGRGGRPRYTVTVEDNGIGVPVTSMAMAFGKVLFSSKYVIRQTRGMYGLGVKAAILYGQM 208

Query: 210 STGLPIEISSSMKGQNYISFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWT 269
           + G P+E+ S+ KG  Y+   +L ID+ RN P I  + + + +  WHG  + + +EG+W+
Sbjct: 209 TAGTPVEVYSATKGSQYVYMMKLVIDVQRNEPRILERGEWNKRGSWHGTRVTLSLEGDWS 268

Query: 270 TYRSKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAV 329
             +SKIL Y+++ AVI PYA+ +     +TPD  I   F R T  +P  P E+K HP  V
Sbjct: 269 RAKSKILEYIKRTAVIAPYAEIIL----ETPDGEI-YYFPRSTRKLPKPPRESKPHPHGV 328

Query: 330 DLLLIKRLIAETAKQNLLQFLQHEFVNIGKSYAERLIGEMG--PEFNSKMSVKSLTSQQI 389
           D+  +K ++  T    L +FL  EF +IG   A+  + + G  P+   +  +K    + +
Sbjct: 329 DIEQLKSMLRSTRASTLREFLVKEFQSIGDKTAQDFLEKYGLDPDLKPRELLKKGRDKLL 388

Query: 390 VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDLVATYSGSAQVFEGHPFIVEA 449
            R+     +  F  P  D LSP G   + +G+ +  +P+ V   S S + + GHPFIVE 
Sbjct: 389 RRLADALMEYPFRAPKSDYLSPIGSDIIEIGLRRMFNPEWVGAVSRSPKAYRGHPFIVEV 448

Query: 450 GVSIGGK-DVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFV 509
           G++ GG  + +    + R+AN+IPLL+E+  D V+   +  INW  Y ++     + V V
Sbjct: 449 GIAYGGGIEARSEPLLLRYANKIPLLYEERED-VSYKVVSSINWRQYNVDFPAPLV-VLV 508

Query: 510 SIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLS 569
            I STK+P+KG GKE +  D+ EI + +++A+Q+   +L+  + +K +  E   R   L+
Sbjct: 509 HIASTKVPYKGVGKESV-SDVPEIEAEIRNAVQEVARRLRLYLSRKAREEEAIRRSVTLA 542

Query: 570 RYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAEILSKVSARLI 611
           +YIP+   ++   L+  ++    K +    +  E L K+ AR I
Sbjct: 569 KYIPEVAVSLAYFLRPPSKWQPPKPEEVK-KIQEALIKIVARHI 542

BLAST of Clc02G24950 vs. ExPASy Swiss-Prot
Match: C3NHT6 (Type 2 DNA topoisomerase 6 subunit B OS=Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2) OX=419942 GN=top6B PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 8.5e-80
Identity = 192/572 (33.57%), Postives = 304/572 (53.15%), Query Frame = 0

Query: 33  SPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNS 92
           SPAEFF  N  +AGF NP + LY TVREL+ENSLD+ +    LP ++ITI+         
Sbjct: 11  SPAEFFKRNPELAGFPNPARALYQTVRELIENSLDATDVHGILPNIKITID--------- 70

Query: 93  MIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGR 152
              L+D  R                                                   
Sbjct: 71  ---LIDEAR--------------------------------------------------- 130

Query: 153 GEASFYRVTCKDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTG 212
                Y+V   DNG G+P  ++PN FGRVL  +KY  +QTRG +GLG K A+++S+M   
Sbjct: 131 ---QIYKVNVVDNGIGIPPQEVPNAFGRVLYSSKYANRQTRGMYGLGVKAAVLYSQMHQD 190

Query: 213 LPIEISSSMKGQNYISFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYR 272
            PIEI +S      I   +L IDI++N P I  +   +N   +HG  + + I G+W   +
Sbjct: 191 KPIEIETSPANSKRIYTFKLKIDINKNEPIIVERGSVENTRGFHGTSVAISIPGDWPKAK 250

Query: 273 SKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLL 332
           S+I  Y+++  +ITPYA+F+FK     P+ N+T  + R T+ +P  P E K HP  VD  
Sbjct: 251 SRIYEYIKRTYIITPYAEFIFK----DPEGNVTY-YPRLTNKIPKPPQEVKPHPYGVDRE 310

Query: 333 LIKRLIAETAKQ-NLLQFLQHEFVNIGKSYAERLIGEMGPEFNSKMSVKSLTSQQIVRIH 392
            IK LI    +   + +FL +EF +IG + A++++   G + N K  VK+LT ++I R+ 
Sbjct: 311 EIKILINNLKRDYTIKEFLVNEFQSIGDTTADKILELAGLKPNKK--VKNLTEEEITRLV 370

Query: 393 QLFRQAK-FDDPSGDCLSPAGEYNLRLGIIKELHPDLVATYSGSAQVFEGHPFIVEAGVS 452
           + F++ + F  PS D LS  GE  + LG+ K  +PD  A+ +   + ++GHPFIVEAGV+
Sbjct: 371 ETFKKYEDFRSPSADSLSVIGEDLIELGLKKIFNPDFAASITRKPKAYQGHPFIVEAGVA 430

Query: 453 IGGK-DVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIV 512
            GG   V +   + R+AN+IPL++++ +DV+ +  ++ ++W  Y I   Q ++ V V + 
Sbjct: 431 FGGSIPVGEEPIVLRYANKIPLIYDEKSDVIWK-VVEELDWKRYGIESDQYQMVVMVHLC 490

Query: 513 STKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNL---S 572
           STKIP+K  GKE I  ++ +I   +K+A+ +   +LK  + +K   R++QE K+ L    
Sbjct: 491 STKIPYKSAGKESIA-EVEDIEKEIKNALMEVARKLKQYLSEK---RKEQEAKKKLLAYL 504

Query: 573 RYIPDATNAVYNVL----KEMAQVHASKKKRG 595
           +YIP+ + ++   L    KE+   + ++   G
Sbjct: 551 KYIPEVSRSLATFLASGNKELVSKYQNEISEG 504

BLAST of Clc02G24950 vs. ExPASy TrEMBL
Match: A0A5A7TIV7 (DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE=3 SV=1)

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 658/672 (97.92%), Postives = 667/672 (99.26%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 YAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF+ KMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQVHASKKKR DCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAEPKEE+YIQSLDAT NFT
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLV 673
           DFQSPLFVFRL+
Sbjct: 661 DFQSPLFVFRLI 672

BLAST of Clc02G24950 vs. ExPASy TrEMBL
Match: A0A1S3BM58 (DNA topoisomerase 6 subunit B OS=Cucumis melo OX=3656 GN=LOC103491545 PE=3 SV=1)

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 658/672 (97.92%), Postives = 667/672 (99.26%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 YAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF+ KMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQVHASKKKR DCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAEPKEE+YIQSLDAT NFT
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLV 673
           DFQSPLFVFRL+
Sbjct: 661 DFQSPLFVFRLI 672

BLAST of Clc02G24950 vs. ExPASy TrEMBL
Match: A0A5D3DDA2 (DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE=3 SV=1)

HSP 1 Score: 1270.4 bits (3286), Expect = 0.0e+00
Identity = 653/691 (94.50%), Postives = 661/691 (95.66%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDN----------- 60
           ME GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDN           
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNVSCIQLLSLSL 60

Query: 61  --------------PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120
                         PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG
Sbjct: 61  SLSPSLSLSVHFLRPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120

Query: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180
           LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA
Sbjct: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180

Query: 181 SFYRVTCKDNGRGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240
           SFYRVTCKDNG+GMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI
Sbjct: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240

Query: 241 EISSSMKGQNYISFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300
           EISSSMKGQNY SFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI
Sbjct: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300

Query: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360
           LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK
Sbjct: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360

Query: 361 RLIAETAKQNLLQFLQHEFVNIGKSYAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFR 420
           RLIAETAKQNLLQFLQHEFVNIGKS+AERLIGEMGPEF+ KMSVKSLTSQQIVRIHQLFR
Sbjct: 361 RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420

Query: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKD 480
           QAKFDDPSGDCLSPAGEYNLRLGIIKELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKD
Sbjct: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480

Query: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540
           VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF
Sbjct: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540

Query: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600
           KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA
Sbjct: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600

Query: 601 VYNVLKEMAQVHASKKKRGDCEDAEILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYA 660
           +YNVLKEMAQVHASKKKR DCEDAEILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYA
Sbjct: 601 IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA 660

Query: 661 TQSGASAEPKEEIYIQSLDATYNFTDFQSPL 667
           TQSGASAEPKEE+YIQSLDAT NFTDFQSPL
Sbjct: 661 TQSGASAEPKEEMYIQSLDATDNFTDFQSPL 691

BLAST of Clc02G24950 vs. ExPASy TrEMBL
Match: A0A6J1DJZ7 (DNA topoisomerase 6 subunit B OS=Momordica charantia OX=3673 GN=LOC111021237 PE=3 SV=1)

HSP 1 Score: 1270.0 bits (3285), Expect = 0.0e+00
Identity = 647/673 (96.14%), Postives = 661/673 (98.22%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTP+KPKES+LKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPKKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRER+D  LYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERIDAALYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKK REPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKVREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PHIHIQEK DNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQF+FKFVSDTP
Sbjct: 241 PHIHIQEKCDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFIFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAET KQNLLQFLQHEFVNIGKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETPKQNLLQFLQHEFVNIGKS 360

Query: 361 YAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEF+ KMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGI+
Sbjct: 361 YAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIV 420

Query: 421 KELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KEL P++VATYSGSAQVFEGHPFIVEAGVS+GGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELQPEMVATYSGSAQVFEGHPFIVEAGVSVGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINW+SYK+NQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKMNQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKR + EDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHESEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFT 660
           ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEP+EEIYIQSL+AT NFT
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPREEIYIQSLEATDNFT 660

Query: 661 DFQSPLFVFRLVY 674
           DFQSPLFVFRLVY
Sbjct: 661 DFQSPLFVFRLVY 673

BLAST of Clc02G24950 vs. ExPASy TrEMBL
Match: A0A6J1H1C2 (DNA topoisomerase 6 subunit B OS=Cucurbita moschata OX=3662 GN=LOC111459466 PE=3 SV=1)

HSP 1 Score: 1267.3 bits (3278), Expect = 0.0e+00
Identity = 647/673 (96.14%), Postives = 662/673 (98.37%), Query Frame = 0

Query: 1   MEVGGGSESPDEPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME+GGGSESPDEPKKGKSKTPRKPKES+LKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKKGREPAASKGMKGRGEASFYRVTC+DNGRGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300
           PH+H+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLFKFVSDTP
Sbjct: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GKS
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 YAERLIGEMGPEFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           YAERLIGEMGPEF  KMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDLVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPD+VATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMA VHASKKKR D E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATYNFT 660
           ILSKVSAR+ITNETLRDKLAQHVEQVDHEMAMEYATQSGASAE KEEIYIQSLDAT NFT
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLVY 674
           DF+SPLFVFRLV+
Sbjct: 661 DFKSPLFVFRLVH 673

BLAST of Clc02G24950 vs. TAIR 10
Match: AT3G20780.1 (topoisomerase 6 subunit B )

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 545/662 (82.33%), Postives = 604/662 (91.24%), Query Frame = 0

Query: 12  EPKKGKSKTPRKPKESLLKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAES 71
           E KKG SK  +   ES LKQKSPAEFFAENKNIAGFDNPGK LYTTVRELVEN+LDSAES
Sbjct: 8   ETKKGSSKNSKDSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAES 67

Query: 72  ISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQA 131
           ISELP VE+TIEEI+K+KFNSMIGL+DRERVD +LYDDYET KAR KRLAKEARA EIQA
Sbjct: 68  ISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQA 127

Query: 132 KNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGRVLSGTKYGLKQ 191
           KN A GKK +EP  SK +K RGEAS+Y+VTCKDNG+GMPHDDIPNMFGRVLSGTKYGLKQ
Sbjct: 128 KNLASGKKNKEPGVSKVLKARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 187

Query: 192 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNIPHIHIQEKRDN 251
           TRGKFGLGAKMALIWSKMSTGLPIEISSSMK QNY++FCRLDIDIHRNIPHIH+ EK+ N
Sbjct: 188 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGN 247

Query: 252 KDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRR 311
           K+ WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+F+S+TP+KN+TI+F+RR
Sbjct: 248 KEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFISETPEKNVTIKFTRR 307

Query: 312 TDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKSYAERLIGEMGP 371
           TDVMPP+P+ETKHHPS+VDLLLIKRLI +T+K+ LLQFLQ+EFVNI K+ A RLIGEMGP
Sbjct: 308 TDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGP 367

Query: 372 EFNSKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDLVATY 431
           +F   M+VKS+TSQQ+VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPD+VATY
Sbjct: 368 DFGPSMAVKSVTSQQMVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 427

Query: 432 SGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWN 491
           SGSAQVFEGHPFIVEAGVS+GG+DVKQG+NIFRFANRIPLLFEQGADVVTRTALKRINWN
Sbjct: 428 SGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWN 487

Query: 492 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVK 551
           SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIA+AVKSAIQQCCIQLKSKIVK
Sbjct: 488 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVK 547

Query: 552 KIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRGDCEDAEILSKVSARLIT 611
           ++QAREQQERKR+LSRYIPDAT AVY VLK+M + H +K+KR   ED  +L KVS ++IT
Sbjct: 548 RLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKRYGEEDIVMLDKVSKQIIT 607

Query: 612 NETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDAT-YNFTDFQSPLFVFR 671
            ETL++KLA+HVEQVD+EMA+EYATQSG S EP+E IY+Q LD    NF D  SP FVFR
Sbjct: 608 KETLKEKLAEHVEQVDYEMALEYATQSGVSEEPRENIYLQHLDPNKSNFIDLHSPTFVFR 667

Query: 672 LV 673
           L+
Sbjct: 668 LM 669