Clc02G08260 (gene) Watermelon (cordophanus) v2

Overview
NameClc02G08260
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptiontRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
LocationClcChr02: 9447542 .. 9452645 (-)
RNA-Seq ExpressionClc02G08260
SyntenyClc02G08260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCCCCTTCCAATTCTCACCTTCACTTCAAAACTATATTCTCTCTCCCTAAGTCTCTCATGGCTTCCTCTTTCGCACAAACCCTTAAACCAGATTTACCTCAACACCTAGACGACCTTCTCTGCTCCGATCCACAGAAACAGCCACAAGAGGAATGCTCCTCTCTTACCAAATCCAATGGCTCAACACTTCTCTTGGGGTCGCTGAGTGGATATTTGAGTGGCGAGTCGCGCATTGAGCGAGCCTGGGCTCACTGGACGAATCTCGGTCGGCCAAAGTTGATTGTAGCTCCAATGGTTGATAATTCAGAGCTACCGTTTCGTTTGCTCTGCAGGAAATATGGTGCTGAGGCAGCGTATACACCCATGCTGCATTCACGGATCTTTACTGAGAACGAGAAGTATCGGAACATGGAATTTACCACTTGTCAGGTAAGTGACAATTTAAAGCCATTCTATTGTGTTGACAACTTGAATTTTCTGTTTCATTTGGTAATTGATGTATTGAATCTATTGATGCATGTGTCCAGCTGCTATTTGTTGATCAAAAGCTGATTTTAGGTTATAATCTTGTCTGTATTATTGTTAAAGTCTATTCTTCATTGTTTTTGAATTTTGGAATGATTTGTAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTAATCTCTATTTGTCTAATTTTTATGGATGTTTTCATTCCTTATGGCCCTATATTCCAAATGCTGACTTCTTGTGCTACTCGCGGGTCGGTGGAGTAAGGATTTGTTTTCCAAAAGACTTTTTTGTTTGTTAAGATTGTGGATTGTTCCCAAGTACTTTTTTGAATAAGTAACAAAACTACAAAAGAAGGGAGCCCCCAATTGGAGGATTTACAAAAGACTTCTCCAATTAGAGAGGAGGAAAGAAAAGCTATAGTAATGGAAAAGAGGGGAGCATTTACTCCAAGTCACACAAACTAGCTATAGTCTTAATAAATCCTTAAAAGATTCTTCTATTATGTTGTTGCCAAATGGTCTGCAAAGAGCTCTCACAAAATGTTCCTACACAAGCTTCTTTTTCCTTTTTGAAGGAATGATCGCTCGAAGTATAAGCAATGAAGATCTTTGGGTCCTCTGGTAGGGCCATAGCCCATATGCCAAAGATGGAGTTCTATTGGTGATTCTAAAATTTCTTGCAAAAACACAAGCAGTGAAGAGATGATTTTGGGATTCCAAGCCATTTTACAAATAATGCACTGCACCATTGGGGAGATAAATTCTGATTAGGAAGTCTTCTCTATAGCTTGTCATTTGTGTTGATGGCCTTTTGTGTAGGTCCCAGATGAAGTTTTTTTTTGGGAAATGTTCTTTCCAAATAAATCATATAGAGGAGAAGTCTCATTGCCAGCTTAATGGAGATATTTTAGGAGGGAACTGGTTGAAAAGACACCTTTTTCTCCAGTTCCCAAATACCTCTGTCCAGTCATTGGGATAAAGTGACATCCTCACGGATTTGCACAATGAAGGATAACCCTCAATGTGAGGTTCCCTCAGTTATCTTCTCAGCTAAAGGTTCCATCAAACATTTTCCATAGACATGTCAATAGTGGCCCCATCCTTCCACTGAGAGATATCAAATATAAGAGGAAATCTTCTATAAAGAGGTTCATCTCCATGCCAAACATCCTTCCGAAAAGATAAATCCAAAGCATCCCCAACAATATAGTTCATTTTGTCATGGATAATGGGACAGTGTTTCAAAATGGTCTTCCAAGGTCCATTGGCCAAAGATTGAGTTGATTGCCTCGGTTTTGAGAAGCATGTTGATGACCTGTATTTTGCATCAATTCCTTTCCTCCAAAGAGCAGGTTTTCACATTGATATTGCCAAATCCATTTTGCAAAGAGAGCATAATTTTTGTCCTTTAGTTTAGACTATGTAAGTCAAGACTGCCATTCTCAAAAGGAAGGAGGAGCTTTCCCATTTAACAAGATGCAATTCCTTACCGTCCATTACCCTTCCAAATGAAATTAAGCATCAGTCTCTCAAGGCCATTTTGAAGATTGACAAGTAATATGTGGGTAGTGATCACCTACATAGTGATTTGGACTCCTATGAGCATCATTTTTATTGGCCATCCCTTCAAGGAGGACAAGAAGATTTTATGGTTAAACTTTAATCATGCCTTTCTAGTGTTTTAGAAAAACACTCTCAAGTGTGCCTAGTTCAAGGCCCAAGTATGGCGCCTTGTCTTGAAAAGGCAAGGCTCACAAAATAAGGCATGCCTCAGGCGCACGCTTTTTGTGAAGCCTGAAGGCTTAAAGCCTTGAACTTTAGGGCTTTTTCATTATTTTAAAAAAATAGCAATAATTATGGTTTTTCCTTTGTTATTAACTAAAAAAATCAAATTTACTAAGCCTAAATGCAAAATTCCTTGTGTTCAAGGGTCTTTTTTTTATTTTTTTTTTATTTTTTATTTTTTATTTTTTATTTTTGCTTGAACTATGCTTTCTCTTCTTTAAGTAGTACTTTATATATAGTGCTCCTTACAAAAAAAAAAAAAAAAAAAACTGCATTTTGTTGTGTCTCAGTGAAAGTTATTATTTTTTTTAAAAAAAACATTTGGTTGATACTTTGGTTTGAAGTTACGACATGTCAATATTTCAGTGATGTTATTAGTTTGACATAGGTGCTATGAGGATACCATATTGACTCTAGACACATATACCCTTTTTCGTTGCATGTAGCACCTTTTTTTTTTTTTTTGGGAGATAATTGGCGAGAAATTTCAATGCTTTTGATTGATGTCTATTGGGTATCCAAACACATAGAATTCAAGAAAATACCAAAAATAACTCTATTGCAATATCAGCGGAGAAATTACAATATCAATAGCTTTTCGAGAGGGTCATCTCTCTCTGGTTCTACAGTGAACAATCTCATCAAATTAATCCACAACCTAAAATCTCTAACTCTCTCCTCTTTATTTATAACCCAGACCCCTATCTAATTACCACTATACCCTTTCCTAATATTCCCAATATATCACAGTGTCTTACTTAGACATGCTCTTACTTAGTCATGGAGGTCTAAACAAAGCTGACTGAACTTCATGGCTAAGTAATCAACTTTTGATAGTTCTACAGATTTAATTAGTTTCAGGCTATTATGGGTGTAAATGTGACACCCTTTTAGCAATTATAGTCTCTCTTCTCTTATTTCAAATTGGAGATCATTTTTGTAACACACCTGTAAGTGGTTGGGAGTCTTTTAACTCTATTTATTTCAAATTTGTTTCTCTTCTGTGAAGACCCTTAAATGACTAAGGTAGCTTTTACATATAACTTCTAACTGGTCTAGAAAATAAAATTGGGAACTTTTTGAAAAGAAATTCATTCATGGCTTATTTTATCCTTTTTGTGCTATTTTCTGCATTGTTCAGTTCTCCACTTCAGTTGCGTCTAAACTAGGGATAAAAGGAAAGAAAGAAAAGAAAAGAAAAGGGAAAAAGTAAAAGAAAGAAAGAACGGGAGGGGAACAAACCAGTCAGTTTGGGTGGATAACAGTATAGGTTTTCAATCAAGAAAAACTGAACATATTCGGTCCTGAGTTTGGCAAAGCTGAACCGAATATACTTGAATCCGATCAAATATATATACACATTTAAGTTTGAATAGTTTGCAAAGGAATGGAAGTGGTGCAGCTGGTGAAAAATTTGCTCTCATATCCTCGTGGGCATGAGTTTGATCCTCCGAGCTCTGGATTGCATCTTTTTAAGTATATATTGGTGGTTTTCCTTCTGTTCAGCCAATTTTAGACAGCCCATTTAAAGCTCACAGGCCCAACGCATATGCCCTTTGCCATTAACAAATTGTGCACTGCTTCAAACTGATAAACTAATGGTTTGGTCGGTTTGGTTATGTATATGTGTGATGATTCGGTTTGATTTTTAAATTTCTGTAAATGTGTTGCTGTTTGGTTTTGATTTGACGTTCAAACCGAACCGATTACACCCCTAGTCTGAACTAACATAAACAATCAAGGATGATCTGAATTTATGTGCTTTCCAAATGTGTTGCATACCAAATATATAATGATGATGGGTGTCTGTTATGTACTTAAGCACCTTGGAGAACCACACCCCAAAAGTTAGTTATTGAGGTGGGAGAGCCAAGCCACTTAAGTACCACATTGGTCATCCCATTCTAACTAATGTGGGTCAAAGGTAGCCCATACTACCTTTTTCTAACCGTGTCCATATTTTTCAGTTAAGGTTTCTGAACTTCAATTGAGTGGTCTAGTAATTTCATGCAGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAGCTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATTTTCCCAAATCTACAAGATACAATTAATTATGCAAGAATGTTAGAGGAAGCTGGTTGCTCCCTTTTAGCTGTCCATGGCCGCACAAGAGATGAAAAAGATGGTAAGAAATTCCGAGCTAATTGGAGTGCCATTAGTGCTGTTAAAAACGCTGTACGAATTCCGGTTCTTGCCAATGGGAATATAAGGCACATGGAGGATGTTAACAACTGTTTGCAAGAGACTGGGGTTGAGGGTGTGCTTTCAGCAGAAACCCTTCTGGAGAATCCTGCACTTTTTGCTGGGTTTCGAACTGCCGAATGGATAGAGGGCTGCGAAGAAAATACCCGAGATGGTAACCTGGATCAAGCCGATTTGCTGGTGGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCACGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATCAAGGATTCTCATGCATCTGCAATTTCATCCAATCCTTGA

mRNA sequence

ATGAGATCCCCTTCCAATTCTCACCTTCACTTCAAAACTATATTCTCTCTCCCTAAGTCTCTCATGGCTTCCTCTTTCGCACAAACCCTTAAACCAGATTTACCTCAACACCTAGACGACCTTCTCTGCTCCGATCCACAGAAACAGCCACAAGAGGAATGCTCCTCTCTTACCAAATCCAATGGCTCAACACTTCTCTTGGGGTCGCTGAGTGGATATTTGAGTGGCGAGTCGCGCATTGAGCGAGCCTGGGCTCACTGGACGAATCTCGGTCGGCCAAAGTTGATTGTAGCTCCAATGGTTGATAATTCAGAGCTACCGTTTCGTTTGCTCTGCAGGAAATATGGTGCTGAGGCAGCGTATACACCCATGCTGCATTCACGGATCTTTACTGAGAACGAGAAGTATCGGAACATGGAATTTACCACTTGTCAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAGCTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATTTTCCCAAATCTACAAGATACAATTAATTATGCAAGAATGTTAGAGGAAGCTGGTTGCTCCCTTTTAGCTGTCCATGGCCGCACAAGAGATGAAAAAGATGGTAAGAAATTCCGAGCTAATTGGAGTGCCATTAGTGCTGTTAAAAACGCTGTACGAATTCCGGTTCTTGCCAATGGGAATATAAGGCACATGGAGGATGTTAACAACTGTTTGCAAGAGACTGGGGTTGAGGGTGTGCTTTCAGCAGAAACCCTTCTGGAGAATCCTGCACTTTTTGCTGGGTTTCGAACTGCCGAATGGATAGAGGGCTGCGAAGAAAATACCCGAGATGGTAACCTGGATCAAGCCGATTTGCTGGTGGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCACGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATCAAGGATTCTCATGCATCTGCAATTTCATCCAATCCTTGA

Coding sequence (CDS)

ATGAGATCCCCTTCCAATTCTCACCTTCACTTCAAAACTATATTCTCTCTCCCTAAGTCTCTCATGGCTTCCTCTTTCGCACAAACCCTTAAACCAGATTTACCTCAACACCTAGACGACCTTCTCTGCTCCGATCCACAGAAACAGCCACAAGAGGAATGCTCCTCTCTTACCAAATCCAATGGCTCAACACTTCTCTTGGGGTCGCTGAGTGGATATTTGAGTGGCGAGTCGCGCATTGAGCGAGCCTGGGCTCACTGGACGAATCTCGGTCGGCCAAAGTTGATTGTAGCTCCAATGGTTGATAATTCAGAGCTACCGTTTCGTTTGCTCTGCAGGAAATATGGTGCTGAGGCAGCGTATACACCCATGCTGCATTCACGGATCTTTACTGAGAACGAGAAGTATCGGAACATGGAATTTACCACTTGTCAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAGCTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATTTTCCCAAATCTACAAGATACAATTAATTATGCAAGAATGTTAGAGGAAGCTGGTTGCTCCCTTTTAGCTGTCCATGGCCGCACAAGAGATGAAAAAGATGGTAAGAAATTCCGAGCTAATTGGAGTGCCATTAGTGCTGTTAAAAACGCTGTACGAATTCCGGTTCTTGCCAATGGGAATATAAGGCACATGGAGGATGTTAACAACTGTTTGCAAGAGACTGGGGTTGAGGGTGTGCTTTCAGCAGAAACCCTTCTGGAGAATCCTGCACTTTTTGCTGGGTTTCGAACTGCCGAATGGATAGAGGGCTGCGAAGAAAATACCCGAGATGGTAACCTGGATCAAGCCGATTTGCTGGTGGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCACGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATCAAGGATTCTCATGCATCTGCAATTTCATCCAATCCTTGA

Protein sequence

MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDLPQHLDDLLCSDPQKQPQEECSSLTKSNGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAISSNP
Homology
BLAST of Clc02G08260 vs. NCBI nr
Match: XP_038901742.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida])

HSP 1 Score: 815.5 bits (2105), Expect = 2.2e-232
Identity = 397/422 (94.08%), Postives = 402/422 (95.26%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDLPQHLDDLLCSDPQKQPQEECSSLTKS 60
           MRSP NSHLHFKTIFS   SLMASSF QTLKPDLPQ LDD LCSDPQ Q Q+ECSS TKS
Sbjct: 1   MRSPYNSHLHFKTIFSFRSSLMASSFTQTLKPDLPQDLDDRLCSDPQNQQQQECSSFTKS 60

Query: 61  NGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           N STLLL S SGYL GESRIERAWAHW  LGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  NDSTLLLASPSGYLCGESRIERAWAHWRKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEE 240
           GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIF NLQDTINYARMLE+
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFSNLQDTINYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVR+PVLANGNIRHMEDV NCLQETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRVPVLANGNIRHMEDVYNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMI 360
           EGVLSAETLLENPALFAGFRTAEWI+G EENTRDGNLDQADLLVDYLK CEKYPVPWRMI
Sbjct: 301 EGVLSAETLLENPALFAGFRTAEWIKGHEENTRDGNLDQADLLVDYLKFCEKYPVPWRMI 360

Query: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIS 420
           RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAI+
Sbjct: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIA 420

Query: 421 SN 423
           SN
Sbjct: 421 SN 422

BLAST of Clc02G08260 vs. NCBI nr
Match: XP_008462837.1 (PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis melo])

HSP 1 Score: 804.7 bits (2077), Expect = 3.9e-229
Identity = 398/425 (93.65%), Postives = 404/425 (95.06%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDL-PQHLDDLLCSDPQKQPQEECSSLT- 60
           MRS SNSHL+ KTIFSL  S MASSF QTLKPDL PQ LDDLLCS PQKQ Q+E SSLT 
Sbjct: 1   MRSLSNSHLYLKTIFSLRNSFMASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTG 60

Query: 61  -KSNGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SN S LLLGS +GYLSGESRIERAWAHWT LGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  SNSNDSKLLLGSPTGYLSGESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           +AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 DAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 241 LEEAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300
           LE+AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEWIEG EENTRDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHAS 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDSH  
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVP 420

Query: 421 AISSN 423
           AISSN
Sbjct: 421 AISSN 425

BLAST of Clc02G08260 vs. NCBI nr
Match: XP_022944464.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata])

HSP 1 Score: 804.3 bits (2076), Expect = 5.1e-229
Identity = 391/421 (92.87%), Postives = 403/421 (95.72%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDLPQHLDDLLCSDPQKQPQEECSSLTKS 60
           MRSPSNSHLH K+I SL KS MASSF QT   D PQ LDDLLCSDP  QPQ+ECSSLTKS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DFPQDLDDLLCSDPHTQPQDECSSLTKS 60

Query: 61  NGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           N STLLLGS SGYLSGESRIERAWAHW+ LGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  NDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEE 240
           GCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE+
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV+NCLQETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMI 360
           EGVLSAETLLENPALFAGFRTA+W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRMI
Sbjct: 301 EGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMI 360

Query: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIS 420
           RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVKIPLYIKDS ASAIS
Sbjct: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPASAIS 418

Query: 421 S 422
           S
Sbjct: 421 S 418

BLAST of Clc02G08260 vs. NCBI nr
Match: KAG7011024.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 803.5 bits (2074), Expect = 8.6e-229
Identity = 391/421 (92.87%), Postives = 402/421 (95.49%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDLPQHLDDLLCSDPQKQPQEECSSLTKS 60
           MRSPSNSHLH K+I SL KS MASSF QT   D PQ LDDLLCSDP  QPQ+ECSSLTKS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DFPQDLDDLLCSDPHTQPQDECSSLTKS 60

Query: 61  NGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           N STLLLGS SGYLSGESRIERAWAHW+ LGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  NDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEE 240
           GCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTI YARMLE+
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTITYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV+NCLQETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMI 360
           EGVLSAETLLENPALFAGFRTA+W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRMI
Sbjct: 301 EGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMI 360

Query: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIS 420
           RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDS ASAIS
Sbjct: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAIS 418

Query: 421 S 422
           S
Sbjct: 421 S 418

BLAST of Clc02G08260 vs. NCBI nr
Match: XP_023512932.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 802.0 bits (2070), Expect = 2.5e-228
Identity = 392/421 (93.11%), Postives = 403/421 (95.72%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDLPQHLDDLLCSDPQKQPQEECSSLTKS 60
           MRSPSNSHLH K+I SL KS MASS AQT   DLPQ LDDLLCSDP  Q Q+ECSSLTKS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSRAQT---DLPQDLDDLLCSDPHTQRQDECSSLTKS 60

Query: 61  NGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           N STLLLGS SGYLSGESRIERAWAHW+ LGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  NDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEE 240
           GCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE+
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV+NCLQETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMI 360
           EGVLSAETLLENPALFAGFRTA+W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRMI
Sbjct: 301 EGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMI 360

Query: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIS 420
           RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDS ASAIS
Sbjct: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAIS 418

Query: 421 S 422
           S
Sbjct: 421 S 418

BLAST of Clc02G08260 vs. ExPASy Swiss-Prot
Match: Q8K582 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 7.7e-79
Identity = 141/294 (47.96%), Postives = 192/294 (65.31%), Query Frame = 0

Query: 90  LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTTCQED 149
           LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    C ED
Sbjct: 14  LGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDVCPED 73

Query: 150 RPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIV 209
           RPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG YGAFL +   L++ ++
Sbjct: 74  RPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGRYGAFLQEEWDLLQRMI 133

Query: 210 EKLASNLQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRDEKDGKKFRANWSA 269
                 L VPV+CKIR+FP +  T+ YA+MLE+AGC LL VHGRT+++K      A+W  
Sbjct: 134 LLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTASWEH 193

Query: 270 ISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEG 329
           I AV+ AV IPV ANGNIR ++DV  C+Q+TGV+GV+SAE  L NPALF G   A W   
Sbjct: 194 IKAVRKAVGIPVFANGNIRCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAVW--- 253

Query: 330 CEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 382
                        +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 254 -------------ELADEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

BLAST of Clc02G08260 vs. ExPASy Swiss-Prot
Match: Q8C2P3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.3e-78
Identity = 140/294 (47.62%), Postives = 193/294 (65.65%), Query Frame = 0

Query: 90  LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTTCQED 149
           LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    C ED
Sbjct: 14  LGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDVCPED 73

Query: 150 RPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIV 209
           RPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG+YGAFL +   L++ ++
Sbjct: 74  RPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQEEWDLLQRMI 133

Query: 210 EKLASNLQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRDEKDGKKFRANWSA 269
                 L VPV+CKIR+FP +  T+ YA+MLE+AGC LL VHGRT+++K      A+W  
Sbjct: 134 LLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTASWEH 193

Query: 270 ISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEG 329
           I AV+ AV IPV ANGNI+ ++DV  C+Q+TGV+GV+SAE  L NPALF G   A W   
Sbjct: 194 IKAVRKAVGIPVFANGNIQCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAVW--- 253

Query: 330 CEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 382
                        +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 254 -------------ELAEEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

BLAST of Clc02G08260 vs. ExPASy Swiss-Prot
Match: Q6P1R4 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS1L PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.8e-78
Identity = 139/288 (48.26%), Postives = 190/288 (65.97%), Query Frame = 0

Query: 96  IVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTTCQEDRPLFVQ 155
           +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    C EDRPL VQ
Sbjct: 20  VVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCEVCPEDRPLIVQ 79

Query: 156 FCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASN 215
           FCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG+YGAFL D   L++ ++      
Sbjct: 80  FCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQDEWDLLQRMILLAHEK 139

Query: 216 LQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKN 275
           L VPV+CKIR+FP +  T+ YA+MLE+AGC LL VHGRT+++K      A+W  I AV+ 
Sbjct: 140 LSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPLSGAASWEHIKAVRK 199

Query: 276 AVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGCEENTR 335
           AV IPV ANGNI+ ++DV  CL++TGV+GV+SAE  L NPALF G   A W         
Sbjct: 200 AVAIPVFANGNIQCLQDVERCLRDTGVQGVMSAEGNLHNPALFEGRSPAVW--------- 259

Query: 336 DGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 382
                  +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 260 -------ELAEEYLDIVREHPCPLSYVRAHLFKLWHHTLQVHQELREE 291

BLAST of Clc02G08260 vs. ExPASy Swiss-Prot
Match: Q9HGN6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus1 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 7.2e-69
Identity = 136/302 (45.03%), Postives = 194/302 (64.24%), Query Frame = 0

Query: 87  WTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFT--TC 146
           +  +GRPK I+APMVD SELP+R+L R+ GA+  Y+PM HSR+F E+E YRN  F+  T 
Sbjct: 12  YNKIGRPKRILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFSTRTI 71

Query: 147 QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVK 206
            E+RPL +QFC NDP+++L+AA+   PYCD VD+NLGCPQ IA++G YG+FL +N  L++
Sbjct: 72  PEERPLIIQFCGNDPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQENWNLIE 131

Query: 207 AIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRDEKDGKKFRAN 266
           +I+ KL + L +PV+ KIRIFP+ Q T++YA+M+ +AG S+LAVHGR R++K      A+
Sbjct: 132 SIITKLHTELSIPVTAKIRIFPDPQKTLDYAKMILKAGASILAVHGRLREQKGHFTGIAD 191

Query: 267 WSAISAV-KNAVRIPVL-ANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTA 326
           W  I  + KN     VL ANGNI H +D++ C++ TGV+GVLSAE  L NP +F      
Sbjct: 192 WEQIQMLRKNLPSETVLFANGNILHAQDIDRCIKYTGVDGVLSAEGSLYNPRIFLP---- 251

Query: 327 EWIEGCEENTRDGNLDQADLLVDYLKLCEKYPV-----PWRMIRSHVHKLLGDWFKIHPH 380
                    T    +D  D+  +YL +  ++ +         I+ H+ KL+     IH  
Sbjct: 252 ---PSSPLMTLYPRID--DMCEEYLNIIREFKLESDYSSLSAIKGHLFKLMRPLLSIHTD 304

BLAST of Clc02G08260 vs. ExPASy Swiss-Prot
Match: P53759 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DUS1 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.6e-63
Identity = 128/312 (41.03%), Postives = 188/312 (60.26%), Query Frame = 0

Query: 90  LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTT---CQE 149
           +GRP  IVAPMVD SEL +R+L R+YGA  AYTPMLH+++F  ++KYR   +++      
Sbjct: 26  IGRPTRIVAPMVDQSELAWRILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDGSSV 85

Query: 150 DRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAI 209
           DRPL VQFCANDP+ LL AA+ VE  CD VDLNLGCPQ IA++G+YG+FLM+   L+  +
Sbjct: 86  DRPLVVQFCANDPEYLLAAAKLVEDKCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLIHNL 145

Query: 210 VEKLASNLQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRDEKDGKKFRANWS 269
           +  L  NL+VPV+ KIRIF + + ++NYA+M+ +AG   L VHGR R++K  K   ANW 
Sbjct: 146 INTLHKNLKVPVTAKIRIFDDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTGLANWE 205

Query: 270 AISAVKNAV--RIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFAGFRTAEW 329
            I  +++ +       ANGNI + ED++ C++  G + V+SAE  L NP +F   +T   
Sbjct: 206 TIKYLRDNLPKETVFFANGNILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVGQT--- 265

Query: 330 IEGCEENTRDGNLDQADLLV----DYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRE 389
                   ++    + D ++      +K C++       ++SH  K+L  +   H  IR 
Sbjct: 266 ------KNKEKIFPRVDKIIREYFQIVKECQESKASKTAMKSHFFKILRPFLPHHTDIRS 325

Query: 390 DF-NAQSKLTFE 392
                 +K T+E
Sbjct: 326 TLATMNAKATWE 328

BLAST of Clc02G08260 vs. ExPASy TrEMBL
Match: A0A1S3CHU6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=LOC103501114 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 1.9e-229
Identity = 398/425 (93.65%), Postives = 404/425 (95.06%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDL-PQHLDDLLCSDPQKQPQEECSSLT- 60
           MRS SNSHL+ KTIFSL  S MASSF QTLKPDL PQ LDDLLCS PQKQ Q+E SSLT 
Sbjct: 1   MRSLSNSHLYLKTIFSLRNSFMASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTG 60

Query: 61  -KSNGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SN S LLLGS +GYLSGESRIERAWAHWT LGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  SNSNDSKLLLGSPTGYLSGESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           +AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 DAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 241 LEEAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300
           LE+AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEWIEG EENTRDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHAS 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDSH  
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVP 420

Query: 421 AISSN 423
           AISSN
Sbjct: 421 AISSN 425

BLAST of Clc02G08260 vs. ExPASy TrEMBL
Match: A0A6J1FWR0 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111448913 PE=4 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.5e-229
Identity = 391/421 (92.87%), Postives = 403/421 (95.72%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDLPQHLDDLLCSDPQKQPQEECSSLTKS 60
           MRSPSNSHLH K+I SL KS MASSF QT   D PQ LDDLLCSDP  QPQ+ECSSLTKS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DFPQDLDDLLCSDPHTQPQDECSSLTKS 60

Query: 61  NGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           N STLLLGS SGYLSGESRIERAWAHW+ LGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  NDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEE 240
           GCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE+
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV+NCLQETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMI 360
           EGVLSAETLLENPALFAGFRTA+W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRMI
Sbjct: 301 EGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMI 360

Query: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIS 420
           RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVKIPLYIKDS ASAIS
Sbjct: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPASAIS 418

Query: 421 S 422
           S
Sbjct: 421 S 418

BLAST of Clc02G08260 vs. ExPASy TrEMBL
Match: A0A0A0LTG2 (Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.1e-228
Identity = 398/425 (93.65%), Postives = 402/425 (94.59%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDL-PQHLDDLLCSDPQKQPQEECSSLTK 60
           MRS SNSHLH K IFSLP SLMASS  QTLKPDL PQ LDDLLCS   KQPQ E SSLT 
Sbjct: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTN 60

Query: 61  --SNGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SN ST LLGS +GYLS ESRIERAWAHWT LGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 241 LEEAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300
           LE+AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVN+CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEWIEG EENTRDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHAS 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDSHA 
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420

Query: 421 AISSN 423
           AISSN
Sbjct: 421 AISSN 425

BLAST of Clc02G08260 vs. ExPASy TrEMBL
Match: A0A6J1JGU3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111484251 PE=4 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 6.0e-228
Identity = 390/421 (92.64%), Postives = 401/421 (95.25%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDLPQHLDDLLCSDPQKQPQEECSSLTKS 60
           MRSPSNSHLH K+I SL KS MASS AQT   D PQ LDDLLCSDP  Q Q+ECSSLTKS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSCAQT---DFPQDLDDLLCSDPHTQRQDECSSLTKS 60

Query: 61  NGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           N STLLLGS SGYL+GESRIERAWAHW+ LGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  NDSTLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEE 240
           GCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE+
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAI AVKNA+RIPVLANGNIRHMEDVNNCLQETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMI 360
           EGVLSAETLLENPALFAGFRTA+W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRMI
Sbjct: 301 EGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMI 360

Query: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIS 420
           RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDS ASAIS
Sbjct: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAIS 418

Query: 421 S 422
           S
Sbjct: 421 S 418

BLAST of Clc02G08260 vs. ExPASy TrEMBL
Match: A0A6J1D4R6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3673 GN=LOC111017278 PE=4 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.4e-224
Identity = 376/422 (89.10%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 1   MRSPSNSHLHFKTIFSLPKSLMASSFAQTLKPDLPQHLDDLLCSDPQKQPQEECSSLTKS 60
           MRS + SHLH K++FSL  SLMASSF+QT+KPDLP+HLDDLLCS+ Q+Q +EECSS+TKS
Sbjct: 1   MRSLAISHLHLKSVFSLHSSLMASSFSQTIKPDLPEHLDDLLCSNQQQQQREECSSVTKS 60

Query: 61  NGSTLLLGSLSGYLSGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           +  +LLLGS +GYLSG+SRIERAWAHW+ LGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  DEPSLLLGSPNGYLSGDSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEE 240
           GCPQRIARRGNYGAFLMDNLPLVKAIV+KL+SNLQVPVSCKIRIFPNLQDT+NYARMLE+
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVQKLSSNLQVPVSCKIRIFPNLQDTLNYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAV+IPVLANGNIRHMEDVNNCLQETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVQIPVLANGNIRHMEDVNNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMI 360
           EGVLSAETLLENPALFAGFRTAEW+EGC+EN RDGNLDQA LLV+YLKLCE+YPVPWR+I
Sbjct: 301 EGVLSAETLLENPALFAGFRTAEWVEGCDENCRDGNLDQAALLVEYLKLCEQYPVPWRII 360

Query: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAIS 420
           RSHVHK+LGDWFKIHPH+REDFNAQ KLTFEFLY MV+RLRELQVKIPLY+KDS AS IS
Sbjct: 361 RSHVHKMLGDWFKIHPHVREDFNAQFKLTFEFLYIMVDRLRELQVKIPLYLKDSPASEIS 420

Query: 421 SN 423
           SN
Sbjct: 421 SN 422

BLAST of Clc02G08260 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 609.0 bits (1569), Expect = 2.9e-174
Identity = 295/399 (73.93%), Postives = 338/399 (84.71%), Query Frame = 0

Query: 19  KSLMASSFAQTL----KPDLPQHLDDLLCSDPQKQPQEECSSLTKSNGSTLLLGSLSGYL 78
           KSL++ + A T     KPD  Q LDD+LCS+ +    EE      ++     LGS S  L
Sbjct: 15  KSLISQTRAMTQNPDPKPDPSQVLDDILCSEQRDGQIEETVDTAPAS-----LGSPSRVL 74

Query: 79  SGESRIERAWAHWTNLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENE 138
           S ++R+ERAWAHW  LGRPK IVAPMVDNSELPFRLLC+KYGA+AAYTPMLHSRIFTE E
Sbjct: 75  SIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETE 134

Query: 139 KYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGA 198
           KYRN EFTTC+EDRPLFVQFCANDPD LLEAA+RVEPYCDYVD+NLGCPQRIARRGNYGA
Sbjct: 135 KYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYGA 194

Query: 199 FLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRD 258
           FLMDNLPLVK++VEKLA NL VPVSCKIRIFPNL+DT+ YA+MLE+AGCSLLAVHGRTRD
Sbjct: 195 FLMDNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTRD 254

Query: 259 EKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPA 318
           EKDGKKFRA+WSAI  VKNA+RIPVLANGN+R +EDV+NC++ETGVEGVLSAETLLENPA
Sbjct: 255 EKDGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLLENPA 314

Query: 319 LFAGFRTAEWI-EGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFK 378
            FAGFRTAEW  +  EE   DG LDQ DL+V+YLKLCEK+PVPWRMIRSHVHK+LG+WF+
Sbjct: 315 AFAGFRTAEWAKDNEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRSHVHKMLGEWFR 374

Query: 379 IHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIK 413
           IHP +RE  NAQ+ LTFEFLY +V++LREL  ++PLY K
Sbjct: 375 IHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKK 408

BLAST of Clc02G08260 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 129.8 bits (325), Expect = 5.2e-30
Identity = 79/247 (31.98%), Postives = 123/247 (49.80%), Query Frame = 0

Query: 94  KLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNME------------- 153
           KL++APMV    L FR+L  +YGA+  Y   +      + E+  N+              
Sbjct: 6   KLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVEKGTDN 65

Query: 154 --FTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLM 213
             F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L+
Sbjct: 66  VVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAALL 125

Query: 214 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRDEKD 273
               L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E+ G   LAVHG  R   D
Sbjct: 126 SKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIAD 185

Query: 274 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALFA 325
             +  A W  I+ V  A+ IPV+ANG++   +D +     TG   V+ A   + N ++F+
Sbjct: 186 RPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIFS 245

BLAST of Clc02G08260 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 112.8 bits (281), Expect = 6.5e-25
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 0

Query: 138 NMEFTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFL 197
           N+ F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L
Sbjct: 26  NVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAAL 85

Query: 198 MDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEEAGCSLLAVHGRTRDEK 257
           +    L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E+ G   LAVHG  R   
Sbjct: 86  LSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIA 145

Query: 258 DGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQETGVEGVLSAETLLENPALF 317
           D  +  A W  I+ V  A+ IPV+ANG++   +D +     TG   V+ A   + N ++F
Sbjct: 146 DRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIF 205

Query: 318 AGFRTAEW 325
           +    + W
Sbjct: 206 SPKGKSHW 211

BLAST of Clc02G08260 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 9.5e-16
Identity = 54/207 (26.09%), Postives = 94/207 (45.41%), Query Frame = 0

Query: 92  RPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 151
           R KL +AP+     LPFR LC+  GA+     M       + +            +    
Sbjct: 337 RDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFG 396

Query: 152 VQFCANDPDVLLEAARRVEPYC--DYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK 211
           VQ C + PD +      ++  C  D++D+N+GCP  +    + G+ L++    +K IVE 
Sbjct: 397 VQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVEV 456

Query: 212 LASNLQVPVSCKIR--IFPNLQDTINYARMLEEAGCSLLAVHGRTRDEKDGKKFRANWSA 271
            +S ++ P++ K+R   F       +    +   G + + +HGR+R ++  K   A+W  
Sbjct: 457 SSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKS--ADWDY 516

Query: 272 I-SAVKNA-VRIPVLANGNIRHMEDVN 293
           I    KNA   + V+ NG++    D N
Sbjct: 517 IYQCTKNATTNLQVIGNGDVYSYLDWN 541

BLAST of Clc02G08260 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 1.2e-10
Identity = 67/249 (26.91%), Postives = 98/249 (39.36%), Query Frame = 0

Query: 93  PKLIVAPMVDNSELPFRLLCRKYGAEA-AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 152
           P   VAPM+D ++  +R L R     A  YT M+ +      +   +       +  P+ 
Sbjct: 52  PSFSVAPMMDWTDNHYRTLARLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHPIV 111

Query: 153 VQFCANDPDVLLEAARRVEPY-CDYVDLNLGCPQ-RIARRGNYGAFLMDNLPLVKAIVEK 212
           +Q   ++ + L +AA+  + Y  D ++LN GCP  ++A  G +G  LM    LV   +  
Sbjct: 112 LQLGGSNVENLAKAAKLSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAMSA 171

Query: 213 LASNLQVPVSCKIRI-------FPNLQDTINYARMLEEAGCSLLAVHGRTRDEKDGKKFR 272
           +A+N  VPV+ K RI       +  L D I     L         VH R          +
Sbjct: 172 IAANTNVPVTVKCRIGVDNHDSYDELCDFIYKVSTLSPT--RHFIVHSR----------K 231

Query: 273 ANWSAISAVKNAVRIPVL------------------ANGNIRHMEDVNNCLQETGVEGVL 314
           A    IS   N  RIP L                   NG I  +  VN  L+E G  GV+
Sbjct: 232 ALLGGISPADNR-RIPPLKYEYYYALVRDFPDLRFTINGGITSVSKVNAALKE-GAHGVM 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901742.12.2e-23294.08tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida][more]
XP_008462837.13.9e-22993.65PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis melo][more]
XP_022944464.15.1e-22992.87tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata][more]
KAG7011024.18.6e-22992.87tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosp... [more]
XP_023512932.12.5e-22893.11tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
Q8K5827.7e-7947.96tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=101... [more]
Q8C2P31.3e-7847.62tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN... [more]
Q6P1R43.8e-7848.26tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=... [more]
Q9HGN67.2e-6945.03tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (st... [more]
P537591.6e-6341.03tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (str... [more]
Match NameE-valueIdentityDescription
A0A1S3CHU61.9e-22993.65tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1FWR02.5e-22992.87tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36... [more]
A0A0A0LTG22.1e-22893.65Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV... [more]
A0A6J1JGU36.0e-22892.64tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661... [more]
A0A6J1D4R62.4e-22489.10tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3... [more]
Match NameE-valueIdentityDescription
AT5G67220.12.9e-17473.93FMN-linked oxidoreductases superfamily protein [more]
AT3G49640.15.2e-3031.98Aldolase-type TIM barrel family protein [more]
AT3G49640.26.5e-2534.04Aldolase-type TIM barrel family protein [more]
AT4G38890.19.5e-1626.09FMN-linked oxidoreductases superfamily protein [more]
AT3G63510.11.2e-1026.91FMN-linked oxidoreductases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 92..324
e-value: 5.0E-80
score: 270.2
IPR035587DUS-like, FMN-binding domainPFAMPF01207Duscoord: 96..387
e-value: 2.7E-73
score: 246.8
IPR035587DUS-like, FMN-binding domainCDDcd02801DUS_like_FMNcoord: 94..324
e-value: 9.06699E-107
score: 312.892
NoneNo IPR availablePANTHERPTHR11082:SF35BNAA09G07510D PROTEINcoord: 74..412
NoneNo IPR availablePANTHERPTHR11082TRNA-DIHYDROURIDINE SYNTHASEcoord: 74..412
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 93..369
IPR018517tRNA-dihydrouridine synthase, conserved sitePROSITEPS01136UPF0034coord: 176..194

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc02G08260.2Clc02G08260.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002943 tRNA dihydrouridine synthesis
biological_process GO:0008033 tRNA processing
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity