Clc02G06880 (gene) Watermelon (cordophanus) v2

Overview
NameClc02G06880
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionLipase
LocationClcChr02: 6947312 .. 6953645 (-)
RNA-Seq ExpressionClc02G06880
SyntenyClc02G06880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTTTGCTAACTAATTTTGCTCACTGTCGCTTGAAGGTCAATCTTTGTGTCGAAGATGACGACGCCATTGATGGCTCTGATATTTCTTCTCTATTTCTTCCCCCTCTACTCCGCCGATGATTCCTCCTACCGTTCCGTCGCTCTGTCCGACAAGTCCTTGTGCTCTCAACTCGTTCAACCTGCTGGCTATCCTTGCGCCGAGCATACGGTACTCTGCTTCACTCATTTCTCTTCTATGTTTGGTCGATTAATTAGTGGATGGTGTTGATTAGGAATTATTTGTGAAATGAGACAGTATTAGTGTTCATCGCTCTGTTTCAAGTGGAGAAACTGGAATGGATTTAGTCAACTAATTGGTTCTAATGCTTCTTGTTTGATATTTTTTTTTTGAGTTTCTTGTTTATTGTGTTTCCTGAGATGAGAATGATGGAATGAATTTATTTTTTATTCTTAGATTCGACGTTCTTCCTCGTTTGATTTGTTTCTGTCAAGTTTCTGGATAAGCCGATGCATTTGTATCGTTCTTTGTGTTTGTAATCGAAAGAATAGGGATAGAGATAATACGCTAAGAAATGTGATTGAATTTGACTCGTATTTAGATCTCTGATTTTATCAATTTCTCATTAGCTATTTGGAGCTTTAACGCATTTCTACCGTATAATTTTGTTGTTATTATTATTATTATTTTAATTTATTATCATTCGCATGAGTTCTGAGGAATGCTATCCATTATTGCAGACTGGGCTGATGAATTGTGCTTTTAGTAGTGCACTTGTACGGTTGTGCAGTACTGACGGACGTGATTTTCTTATAAATTTCTTAGATTGAAACGAAGGATGGCTTTTTATTAGGTCTGCAACGTGTATCTTCACGTGATGAAGATTTGGCAAAGCAAAAAGGCCCTCCAATTTTGCTCCTCCATGGACTGTTCATGGTGAGTCTTCTTACGGTTGCAATGTTGTAACTTTTTTTGGTGGGTCACTTCACTCTTTTATGGTCTAACTCTATCGGCAATAGCTAGGGGATGGATATCGTTGCCTGATATCTTTTATACTACTGGGACTATCTCCTTTCGTTGTAATTTTCAACTGTCCCCATCTCTTGTATTATAGTGAAATGGCTATTTTCATCTCTTATTTGATAAAATTGGAATAATAAAGAGAAGATCAGGATAGCCCCTAGGTGACATGATTATTTTCCCAGAAAAAGTAACTAAGATTCTCTATCAGGTCAATATTTACCTTAAACTTCTACAGTCTAATAGTACAAACATGCTTTCCAGTTGTATCTTAAGGAATTGGTATTGTAAGATGAATGCCATAAAGCCTTGAGGCAGATTTTAGCCAAGGCCCTATCTTATATTTTGGTCTCTGTCTCTCTCTTTTTAATCTACTGGTGTCTGTTTTCTTGTCTTTCCAAGTGAGATTCTTTTCATGTTCTTTTTACACATCTCAAAGTATACTGAATAGTCTTTTTGAGTTTCATTCCTTGGTACAACATTTCTATGCTTGGCAAGATCCAATTATCCATAAATTTTCTCGTCTACGAGATTAATAACTGCATATTTTGAAACATGGATGGTGAATTTTGAATAGTTCTGTAGCCTGGGATCATTCTCACAGATAAGGGGTTCCTACCACTAGCTTTTCATACCTTTCATTGTCTGAACTGGTGCATGTGCAGGGTGGCGATGGATGGTTTTTGAATTCTGCAAGACAATCTTTAGGCTTTATTCTTGCAGATAATGGATTTGATGTATGGATAGGAAATGTGCGTGGAACACGTTGGAGTCATGGGCATACATCCCTATCAGAGGATGAGAAGGTATTCATACATTACTTTATTACAAAATCCCTAAGATGTGTAAGATGTTAATTGAATGCAGAGAAAGAACATATTAATAGAAAGGGTTTCCTCATTGTGTTGTGGGTGTATTGCATGTATCTAGAAAGTTAAATAATGTCACAAACATGCTGTAGCTCATCCATGCTCAATGCATTGAGCTGGAGAGTATCATGTAGTCAAATTTGGCTGTTCAAACTTCACACTTGTGTGAGGGAGGTTTTATGGAGTTCAGCAGTTACACGACAGATTATCTTAAAAGACCATTTGGTTTCATCCTAATTGTCTTCATTTGCCAAAGCCTGTTTTGTCTCAAATTTGTGCACTATACTTTCTGTGGACTACTAGTCCATAAGCATCTCAGGAAAATCTTGGAAGTAATTTAACATAAATTGCGTATTTAGTTTCTTTTATGTCTCTCTAATATATTCTGATTGTAGGAATTTTGGACTTGGAGTTGGGAAGAGTTGGCTCTGTACGATCTTGCAGAAATGATTAACTACATACATTCATTGACAAACAGGAAAATTTATGTTGTTGGACACTCACAGGTCTCATATCTCTTCTATGAGATTCTTAATCTGAAATCATATATTGTTGCTCTTGCTACATCGTCATTTATCTTTGTTTGCAGGGAACAATCATGTCTTTTGCTGCTCTGACCCAGCCAGACATAGCCAAGAAAGTTGAAGCAGCTGCTCTTTTAAGTCCTATATCGTATTTGGAACATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTTGATACGGTAATCCTGGCTTGTTATCGTGTAATTGGCGTTGTTAAGTGATTATAATATTAATAGTAATAAGCTTATTCCTTATATTCTTCTCGGCAGGTAATCCTTGCTGCGGGATTTCGTGAACTCAACTTTAAAAGGTTTTCATGCTATGCTATGTTCAATTTTGATTTGTAATGGGTTGCATTTTTATCTTCACCATAGTTTATAATACTCATGTCTATGTTTTTGTATGCTACTTCAGCGATTGGGGAACAGTCCTTTTAGATAATCTTTGTGATCGGCTGGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGTTCTCTCAATGCAACTTGCTTCATGTTTCTTTCTCCTTTCTTTTTATTTATTTATTTATTTTTGTCAATCCTTGACTTTGGAATCCTTATCCAACATACTTCGACAATCCCTTCTCGAACCAAACATGAATCTTCTGATTCATTTGGCTCTCATGCTCAGTTATTGCAATTATTTTGGGGAAATTCCCATAGATCTTTCTGAATGTCATGAACCTAACCTCTTTTCTTGATTCATATTCCACGAAGAAAAAAAATTGGTCATTAATCAAAGACCCTAATATTGGGAGGACTCTTCTGATCAAACACCTATCCTTTTTTCTTTTTCTTTTTTTCTTATCTTTCTTTCTCTTGAAATCCAAAAAATTTTTCTTACTTTATACATAACATAGGTCATCGATTCAACATTGGATAAAAAATAAAAAAATGAGGGTGAAATCTTGCTAATTTCTTTTAATTACTATAACGTATACTGACAGCAATTATATTGGATCATAATCTTATGTGGTTAGCGGGTACATCTTCTCTTAAAACTGAAAGGCCTAGGTTGGAAAATATTACTAAAATTTACCACGTGATGGAATTTGGAAAATATTGCTGATATTTACCAAGTGCTCCATTTTTCATATGATATTGCTGCCAGAAGGCTTCTTTTAGTCATTAGTGCAATTCACGTAATGGACTACCTCCTTGCTAAACTTCCATTTTTACTCTGATGCACATCTGCATTTTCATCCATTTTATTGAACCTTAACTGCATATGCTATATGGTAGATCATTTTCAGGATTTAAATGCCCTACGTATGGGAAATTTTCAAGTACAGTACTTAATAAAATGTCATGATCCAATATTGATCGAGGTCGGGATTTATATTGTCATGTTTCTTCTTGTAGTTGGAAAAGAATTGGGTACAACTGGAAAAGAAAAATCATAATGAGTTGTTGATTGGAATGACTGTGAAATGTTGCTTTATTCTTCATAAAAAAGTATCAAGTTTGGATGTTCAATCTATTTAATTGCAGACATTAAATTTCCTAAGGATGCCTTCTTTTAATGCTGCAGGGGAAAATTGTTGCCTTAATCGCTCACGTTTTGACTTATTTTTCGAATATGAACCACATCCATCTTCAGCAAAAAATCTGCACCATCTTTTCCAGAGTATGCTCTGCAATATCTTCTCTCTACTTCATGTTCTATTATACTATGACTTCGATGAATCTTTTTTTTTTCTGTTTTCTTTCTTTTTTCTTTCTTTTCTTTTTTTTTTTTTTTTGAGTACTATTGAGGGTGGGGGAATTCAAACCACAGATTTCATGGTTATTGTGGTTATTAATACATTTGCATGCCAATTGAGCTATGCTCAGCTTGACCTCAACAAGTAAGACTTGGGAAGTTCATTTTAGTGGTCCCTTGTGAAGTCTAATATAAATCTATAATCACTCAATTGTGAAACTTTGCATCATTCCAAACTCTTTCCAGAAACCTCCCGGAGATATTAAAAACCCCATCAAAGCGCAACCATATAGAATACTCAGCTTCCCACAAAGAGCTCTTTCATTTTTAACAGTTTACGCCAGACACCAATCTCTTTCACTGGTGACTTCTTCTTCCATCAAATCCCTGGCTTTCCATTAACTTTATCTATAATTAAAGCCAGCCCTCTATTTCCCAAGCAACTTTTATCAATTACCGAACTCATTTTCCCTTTCACTTCCTTGTTCTTACCTTTGCTTTCCACCCTTTGCCTATGCTTTTCAATCAAAGCTACATTTTCTGCTTGCTATTCCCAATCTTCTTGTTTTCTTCACGTTTGCTAGGCACAAACAACATAAACCTTGCTTATTATTCCCAAGCCGTCTTCTTTCGCAGTTCATCTTTGTCGTATTGTACTTCAAACTGGAACATTAGCTATGGTTTAGGATCAATTTTTCTGTTGTTAAATCTGTTAATCTTTCTATCTGCCATATAACTGAACGAAAAACTTCTCCTTTAGCAAAACAAAAACTGATCCTAAACCATCACTAATATCCTGACTTCAACACATGGCAACAATTTACTACACTAAAATTGAATTATTGGTTTGAAGAAATGTTATAGTTTACAAATTGATTTACTTATGCCTTCTTTAGTAACCATTTGGTTTTTGGTTTTTGGTTTTTAGTTTTTAGTTTTTAAAAGTTAAAGCTAATAACCACTCTTTCTACCTCTACATTCTTGTTTTGATATCTACTTTTACCAATATTTTCAAAGCTAAGCTAAGTATTGAAAACTAAAAAAGTACTTTTTAAAAACTTGTTTTTGTTTTTTGAAATTTAGCTAAGAATTTAACTATTTTACGTAAGAAAGATGCAAACCTAAGAAGCACGAATACTTTAGTTTGGTTAGCATGTTTGTGTCCGACGCATGGCGGACACTTGGATACTACGACACTTGTTGGACACGTATTGGACACTTGTTATTGCAACAAATGTGATAGACATGTATCACTTGTTGAGTAGACTAAAACGACACATGCCAATAATAATAACTTTTGAGCGTGAAATATATCCAGCTAAGTTTTTTAAGCAAATAAATGCATCAACCCATTTACTTTGAATTTTCTTTTGGTATAAAAATGATATCTATTTTTAGAAAATGTGTCCTTGCCATGTCGTGTCCCAGATATTTAAAATATGGCGTGGCCATGTCATGTTGTGTTCGTGTCTCGTATCTGTATCTCGTATTCGTATCTATGCTTCTTAGATGCAAACCATTATAAGAAATTGAGAAAAAATAAGATCAATTTTTAAAAACCAAATGATTACCAAACGGAAACTTAGTTACGGGTGTTTTCTCCTCTTGACGAGAATAGGAGGCTGTTCGACACTGCAGATTTAACTGCCTTCCTTTTCCTTTACCAGTGATCCGGAAGGGTACTTTTTCCAGGTATGACTATGGATTGCTAAAGAATCTGAGAGTCTACGGTCAAAGGAAGCCTCCTGAATTCGATCTTAGCCATATTCCCAAGTCCTTGCCACTGTGGATGGCGTATGGTGGCAACGACGAGTTGTCAGATTGGACAGATCTGGAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTAGTTTACCTAGAAAATTATGGTCATGTCGACTTCATTTTGAGCATGACAGCGAAGAAAGATGTGTATGATCCTATGATCAAATTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAAAGTGAATTCTGGTTAATGTCCATTGTAAATGTGTGTTATAAATGTGTGTGTAGTAACAGATCCCTCTGGTCATGGATGTACCTTTATGGAGAGCTAAATGTTCAATATGTTGTGTATTGATTCCTAAACTTTACACAAACAGAAAAGTAATGAAGTAATAAAGAAAATTATTTTTACTA

mRNA sequence

CTCTTTGCTAACTAATTTTGCTCACTGTCGCTTGAAGGTCAATCTTTGTGTCGAAGATGACGACGCCATTGATGGCTCTGATATTTCTTCTCTATTTCTTCCCCCTCTACTCCGCCGATGATTCCTCCTACCGTTCCGTCGCTCTGTCCGACAAGTCCTTGTGCTCTCAACTCGTTCAACCTGCTGGCTATCCTTGCGCCGAGCATACGATTGAAACGAAGGATGGCTTTTTATTAGGTCTGCAACGTGTATCTTCACGTGATGAAGATTTGGCAAAGCAAAAAGGCCCTCCAATTTTGCTCCTCCATGGACTGTTCATGGGTGGCGATGGATGGTTTTTGAATTCTGCAAGACAATCTTTAGGCTTTATTCTTGCAGATAATGGATTTGATGTATGGATAGGAAATGTGCGTGGAACACGTTGGAGTCATGGGCATACATCCCTATCAGAGGATGAGAAGGAATTTTGGACTTGGAGTTGGGAAGAGTTGGCTCTGTACGATCTTGCAGAAATGATTAACTACATACATTCATTGACAAACAGGAAAATTTATGTTGTTGGACACTCACAGGTCTCATATCTCTTCTATGAGATTCTTAATCTGAAATCATATATTGTTGCTCTTGCTACATCGTCATTTATCTTTGGAACAATCATGTCTTTTGCTGCTCTGACCCAGCCAGACATAGCCAAGAAAGTTGAAGCAGCTGCTCTTTTAAGTCCTATATCGTATTTGGAACATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTTGATACGGTAATCCTTGCTGCGGGATTTCGTGAACTCAACTTTAAAAGCGATTGGGGAACAGTCCTTTTAGATAATCTTTGTGATCGGCTGGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGGGAAAATTGTTGCCTTAATCGCTCACGTTTTGACTTATTTTTCGAATATGAACCACATCCATCTTCAGCAAAAAATCTGCACCATCTTTTCCAGATGATCCGGAAGGGTACTTTTTCCAGGTATGACTATGGATTGCTAAAGAATCTGAGAGTCTACGGTCAAAGGAAGCCTCCTGAATTCGATCTTAGCCATATTCCCAAGTCCTTGCCACTGTGGATGGCGTATGGTGGCAACGACGAGTTGTCAGATTGGACAGATCTGGAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTAGTTTACCTAGAAAATTATGGTCATGTCGACTTCATTTTGAGCATGACAGCGAAGAAAGATGTGTATGATCCTATGATCAAATTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAAAGTGAATTCTGGTTAATGTCCATTGTAAATGTGTGTTATAAATGTGTGTGTAGTAACAGATCCCTCTGGTCATGGATGTACCTTTATGGAGAGCTAAATGTTCAATATGTTGTGTATTGATTCCTAAACTTTACACAAACAGAAAAGTAATGAAGTAATAAAGAAAATTATTTTTACTA

Coding sequence (CDS)

ATGACGACGCCATTGATGGCTCTGATATTTCTTCTCTATTTCTTCCCCCTCTACTCCGCCGATGATTCCTCCTACCGTTCCGTCGCTCTGTCCGACAAGTCCTTGTGCTCTCAACTCGTTCAACCTGCTGGCTATCCTTGCGCCGAGCATACGATTGAAACGAAGGATGGCTTTTTATTAGGTCTGCAACGTGTATCTTCACGTGATGAAGATTTGGCAAAGCAAAAAGGCCCTCCAATTTTGCTCCTCCATGGACTGTTCATGGGTGGCGATGGATGGTTTTTGAATTCTGCAAGACAATCTTTAGGCTTTATTCTTGCAGATAATGGATTTGATGTATGGATAGGAAATGTGCGTGGAACACGTTGGAGTCATGGGCATACATCCCTATCAGAGGATGAGAAGGAATTTTGGACTTGGAGTTGGGAAGAGTTGGCTCTGTACGATCTTGCAGAAATGATTAACTACATACATTCATTGACAAACAGGAAAATTTATGTTGTTGGACACTCACAGGTCTCATATCTCTTCTATGAGATTCTTAATCTGAAATCATATATTGTTGCTCTTGCTACATCGTCATTTATCTTTGGAACAATCATGTCTTTTGCTGCTCTGACCCAGCCAGACATAGCCAAGAAAGTTGAAGCAGCTGCTCTTTTAAGTCCTATATCGTATTTGGAACATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTTGATACGGTAATCCTTGCTGCGGGATTTCGTGAACTCAACTTTAAAAGCGATTGGGGAACAGTCCTTTTAGATAATCTTTGTGATCGGCTGGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGGGAAAATTGTTGCCTTAATCGCTCACGTTTTGACTTATTTTTCGAATATGAACCACATCCATCTTCAGCAAAAAATCTGCACCATCTTTTCCAGATGATCCGGAAGGGTACTTTTTCCAGGTATGACTATGGATTGCTAAAGAATCTGAGAGTCTACGGTCAAAGGAAGCCTCCTGAATTCGATCTTAGCCATATTCCCAAGTCCTTGCCACTGTGGATGGCGTATGGTGGCAACGACGAGTTGTCAGATTGGACAGATCTGGAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTAGTTTACCTAGAAAATTATGGTCATGTCGACTTCATTTTGAGCATGACAGCGAAGAAAGATGTGTATGATCCTATGATCAAATTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAA

Protein sequence

MTTPLMALIFLLYFFPLYSADDSSYRSVALSDKSLCSQLVQPAGYPCAEHTIETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIKFFKSLGKSSSL
Homology
BLAST of Clc02G06880 vs. NCBI nr
Match: XP_038886839.1 (triacylglycerol lipase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 742.3 bits (1915), Expect = 2.4e-210
Identity = 367/431 (85.15%), Postives = 383/431 (88.86%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSVALS--------DKSLCSQLVQPAGYPCAEHTI 60
           M TPLM+LIFLL+FFPL+SA +SS+ SV  S         KSLCSQLV PAGYPCAEHTI
Sbjct: 1   MATPLMSLIFLLFFFPLHSAHNSSFSSVEYSILPHARSDQKSLCSQLVHPAGYPCAEHTI 60

Query: 61  ETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFD 120
           +TKDGFLLGLQRVSSRD DL K+KGPPILLLHGLFMGGDGWFLNSA+QSLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSRDGDLEKRKGPPILLLHGLFMGGDGWFLNSAKQSLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQ 180
           VWIGNVRGTRWSHGHTSLSED+KEFW WSWEELALYDLA MINYI+SLT+RKIYVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDDKEFWNWSWEELALYDLAAMINYINSLTDRKIYVVGHSQ 180

Query: 181 VSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240
                                    GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA
Sbjct: 181 -------------------------GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240

Query: 241 PLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNR 300
           PLVRLMVDTHLDT+IL+AGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNR
Sbjct: 241 PLVRLMVDTHLDTIILSAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNR 300

Query: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPK 360
           SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKN+RVYGQRKPPEFDLS IPK
Sbjct: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNMRVYGQRKPPEFDLSRIPK 360

Query: 361 SLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIK 420
           SLPLWMAYGGNDELSDWTDLEHTIKELK VPELVYLENYGHVDFILSM AKKD+YDPMIK
Sbjct: 361 SLPLWMAYGGNDELSDWTDLEHTIKELKPVPELVYLENYGHVDFILSMNAKKDLYDPMIK 406

Query: 421 FFKSLGKSSSL 424
           FFKSL KSSS+
Sbjct: 421 FFKSLAKSSSM 406

BLAST of Clc02G06880 vs. NCBI nr
Match: XP_008454984.1 (PREDICTED: triacylglycerol lipase 1 isoform X1 [Cucumis melo] >KAA0031334.1 triacylglycerol lipase 1 isoform X1 [Cucumis melo var. makuwa] >TYK06787.1 triacylglycerol lipase 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 730.3 bits (1884), Expect = 9.3e-207
Identity = 364/432 (84.26%), Postives = 382/432 (88.43%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV--------ALSD-KSLCSQLVQPAGYPCAEHT 60
           M TPLMAL+FL+YFFPL+SA DSS  +V        ALSD KSLCSQLV PAGYPCAEH 
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGF 120
           I+TKDGFLLGLQRVSSR+ DL KQKGPPILLLHGLFM GDGWFLNSARQSLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHS 180
           DVWIGNVRGTRWSHGH+SLSEDEKEFW WSWEELALYDLAEMINYI+SLTNRKIY+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHIT 240
           Q                         GTIMSFAALTQP+IAK VEAAALLSPISYLEHIT
Sbjct: 181 Q-------------------------GTIMSFAALTQPEIAKNVEAAALLSPISYLEHIT 240

Query: 241 APLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN 300
           APLVRLMVDTHLDT+ILA+GF ELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN
Sbjct: 241 APLVRLMVDTHLDTIILASGFHELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN 300

Query: 301 RSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIP 360
           RSRFDLFFEYEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKNLRVYGQR PPEFDLS IP
Sbjct: 301 RSRFDLFFEYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRMPPEFDLSLIP 360

Query: 361 KSLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMI 420
           +SLPLWMAYGGNDELSDWTDLEHTIK++KSVPELVYLENYGHVDFILSM AK+DVYDPMI
Sbjct: 361 ESLPLWMAYGGNDELSDWTDLEHTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMI 407

Query: 421 KFFKSLGKSSSL 424
           KFFKSLGKSSSL
Sbjct: 421 KFFKSLGKSSSL 407

BLAST of Clc02G06880 vs. NCBI nr
Match: XP_022952782.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 723.4 bits (1866), Expect = 1.1e-204
Identity = 362/431 (83.99%), Postives = 378/431 (87.70%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV-------ALSD-KSLCSQLVQPAGYPCAEHTI 60
           M  PLMA++FLL FF L+SADDSSYRS+        LSD KSLCSQLVQPAGYPCAEH I
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  ETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFD 120
           +TKDGFLLGLQRVSS D DL KQKGPPILLLHGLFMGGD WFLNSAR+SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQ 180
           VWIGNVRGTRWSHGHTSLSEDEKEFW WSWEELALYDLA MI+YI+SLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 VSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240
                                    GTIMSFAALTQPDIA+KVEAAALLSPISYLEHITA
Sbjct: 181 -------------------------GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITA 240

Query: 241 PLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNR 300
           PLVRLMVDTHLDT+ILAAGF ELNFKSDWGTVLLD+LCDRLVNCINVLSSITGENCCLN 
Sbjct: 241 PLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNG 300

Query: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPK 360
           SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPP FDLS IPK
Sbjct: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPK 360

Query: 361 SLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIK 420
           SLPLWMAYGG+DELSDWTDL+HTIKELKSVPELVYLENYGHVDFILSM AK+DVYDPMIK
Sbjct: 361 SLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIK 406

Query: 421 FFKSLGKSSSL 424
           F KSLGKS +L
Sbjct: 421 FLKSLGKSGNL 406

BLAST of Clc02G06880 vs. NCBI nr
Match: XP_023553816.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.0 bits (1865), Expect = 1.5e-204
Identity = 361/428 (84.35%), Postives = 377/428 (88.08%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV-------ALSD-KSLCSQLVQPAGYPCAEHTI 60
           M  PLMA++FLL FF L+SADDSSYRS+       ALSD KSLCSQLVQP GYPCAEH I
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60

Query: 61  ETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFD 120
           +TKDGFLLGLQRVSSRD +L KQKGPPILLLHGLFMGGD WFLNSAR+SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQ 180
           VWIGNVRGTRWSHGHTS+SEDEKEFW WSWE+LALYDLA MI+YI+SLT RKIYVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180

Query: 181 VSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240
                                    GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA
Sbjct: 181 -------------------------GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240

Query: 241 PLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNR 300
           PLVRLMVDTHLDT+ILAAGF ELNFKSDWGTVLLD+LCDRLVNCINVLSSITGENCCLN 
Sbjct: 241 PLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNG 300

Query: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPK 360
           SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPP FDLS IPK
Sbjct: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPK 360

Query: 361 SLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIK 420
           SLPLWMAYGG+DELSDWTDL+HTIKELKSVPELVYLENYGHVDFILSM AK+DVYDPMIK
Sbjct: 361 SLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIK 403

BLAST of Clc02G06880 vs. NCBI nr
Match: XP_023553815.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.0 bits (1865), Expect = 1.5e-204
Identity = 361/428 (84.35%), Postives = 377/428 (88.08%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV-------ALSD-KSLCSQLVQPAGYPCAEHTI 60
           M  PLMA++FLL FF L+SADDSSYRS+       ALSD KSLCSQLVQP GYPCAEH I
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60

Query: 61  ETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFD 120
           +TKDGFLLGLQRVSSRD +L KQKGPPILLLHGLFMGGD WFLNSAR+SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQ 180
           VWIGNVRGTRWSHGHTS+SEDEKEFW WSWE+LALYDLA MI+YI+SLT RKIYVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180

Query: 181 VSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240
                                    GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA
Sbjct: 181 -------------------------GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240

Query: 241 PLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNR 300
           PLVRLMVDTHLDT+ILAAGF ELNFKSDWGTVLLD+LCDRLVNCINVLSSITGENCCLN 
Sbjct: 241 PLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNG 300

Query: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPK 360
           SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPP FDLS IPK
Sbjct: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPK 360

Query: 361 SLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIK 420
           SLPLWMAYGG+DELSDWTDL+HTIKELKSVPELVYLENYGHVDFILSM AK+DVYDPMIK
Sbjct: 361 SLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIK 403

BLAST of Clc02G06880 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 8.6e-139
Identity = 237/415 (57.11%), Postives = 305/415 (73.49%), Query Frame = 0

Query: 8   LIFLLYFFPLYSADDSSYRSVALSDKSLCSQLVQPAGYPCAEHTIETKDGFLLGLQRVSS 67
           L+ +L    ++SA   S+        SLC+ L+ PA Y C EH+I+TKDG++L LQRV+S
Sbjct: 5   LVAVLTSLTIFSALTQSHLLHGSPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVAS 64

Query: 68  RDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGH 127
               L  Q GPP+LL HGLFM GD WFLNS ++SLGFILAD+GFDVW+GNVRGTR+S+GH
Sbjct: 65  LGPRL--QSGPPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGH 124

Query: 128 TSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQVSYLFYEILNLKSYI 187
            +LS+ +KEFW WSW++LA+YDLAEMI Y++S++N KI++VGHSQ               
Sbjct: 125 VTLSDTDKEFWDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQ--------------- 184

Query: 188 VALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTVI 247
                     GTIMSFAALTQP +A+ VEAAALL PISYL+H+TAPLV  MV  HLD ++
Sbjct: 185 ----------GTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPLVERMVFMHLDQMV 244

Query: 248 LAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNRSRFDLFFEYEPHPSS 307
           +A G  ++NF+SD    L+D+LC+  ++C + L+SITG NCC N S+ + + +YEPHPSS
Sbjct: 245 VALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASKIEYYLDYEPHPSS 304

Query: 308 AKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELS 367
            KN+ HLFQMIRKGTF++YDYG  KNLR YG  KPPEF LSHIP SLP+WM YGG D L+
Sbjct: 305 VKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASLPMWMGYGGTDGLA 364

Query: 368 DWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIKFFKSLGKSSS 423
           D TD+EHT+ EL S PEL+YLE+YGH+DF+L  +AK+DVY  MI+FF++  KSSS
Sbjct: 365 DVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFFRAKVKSSS 392

BLAST of Clc02G06880 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.6e-74
Identity = 146/390 (37.44%), Postives = 222/390 (56.92%), Query Frame = 0

Query: 35  LCSQLVQPAGYPCAEHTIETKDGFLLGLQRV-SSRDEDLAKQKG--PPILLLHGLFMGGD 94
           +C+  V   GY C EH + T+DG++L +QR+   R   +A   G   P+L+ HG+ + G 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGM 110

Query: 95  GWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLA 154
            W LN A Q+L  ILAD GFDVW+GN RGTR+S  H  L+  ++ FW W+W+EL  YDL 
Sbjct: 111 SWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLP 170

Query: 155 EMINYIHSLTNRKIYVVGHSQVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDI 214
            M ++IH LT +KI+ +GHS                          GT++ FA+ ++  +
Sbjct: 171 AMFDHIHGLTGQKIHYLGHS-------------------------LGTLIGFASFSEKGL 230

Query: 215 AKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCD 274
             +V +AA+LSP++YL H+T  +  +   T L       G+ E N KS      +  +C 
Sbjct: 231 VDQVRSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICL 290

Query: 275 RL-VNCINVLSSITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGL 334
           +  ++C +++S ITG+NCCLN S  DLF   EP  +S KN+ HL Q +R     +Y+YG 
Sbjct: 291 KAGIDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGS 350

Query: 335 L-KNLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELSDWTDLEHTIKELK----SVPEL 394
             +N++ YGQ  PP +++S IP  LPL+ +YGG D L+D  D+E  + + K        +
Sbjct: 351 SDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNV 410

Query: 395 VYLENYGHVDFILSMTAKKDVYDPMIKFFK 416
            ++++Y H DFI+ +TAK  VY+ +  FFK
Sbjct: 411 QFVKDYAHADFIMGVTAKDVVYNQVATFFK 415

BLAST of Clc02G06880 vs. ExPASy Swiss-Prot
Match: P04634 (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.5e-50
Identity = 126/387 (32.56%), Postives = 203/387 (52.45%), Query Frame = 0

Query: 37  SQLVQPAGYPCAEHTIETKDGFLLGLQRV---SSRDEDLAKQKGPPILLLHGLFMGGDGW 96
           SQ++   GYPC E+ + T+DG++LG+ R+    +  E++ K+  P + L HGL      W
Sbjct: 35  SQMITYWGYPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKR--PVVYLQHGLIASATNW 94

Query: 97  FLNSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEM 156
             N    SL F+LAD G+DVW+GN RG  WS  +   S D  EFW +S++E+A YDL   
Sbjct: 95  IANLPNNSLAFMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPAT 154

Query: 157 INYIHSLTNR-KIYVVGHSQVSYLFYEILNLKSYIVALATSSFIFGTIMSFAAL-TQPDI 216
           IN+I   T + KI+ VGHSQ                         GT + F A  T P +
Sbjct: 155 INFIVQKTGQEKIHYVGHSQ-------------------------GTTIGFIAFSTNPTL 214

Query: 217 AKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTVILAAGFRELNF--KSDWGTVLLDNL 276
           AKK++    L+P++ +++  +PL ++   + + T +    F +  F   + +   L   +
Sbjct: 215 AKKIKTFYALAPVATVKYTQSPLKKI---SFIPTFLFKLMFGKKMFLPHTYFDDFLGTEV 274

Query: 277 CDRLV---NCINVLSSITG-ENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSR 336
           C R V    C N L    G +   LN SRFD++  + P  +S ++  H  Q++R G F  
Sbjct: 275 CSREVLDLLCSNTLFIFCGFDKKNLNVSRFDVYLGHNPAGTSVQDFLHWAQLVRSGKFQA 334

Query: 337 YDYGL-LKNLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELSDWTDLEHTIKELKSVPE 396
           +++G   +N+  Y Q+ PPE+D+S +   + +W   GGND L+D  D+   + +L ++  
Sbjct: 335 FNWGSPSQNMLHYNQKTPPEYDVSAMTVPVAVW--NGGNDILADPQDVAMLLPKLSNLLF 389

Query: 397 LVYLENYGHVDFILSMTAKKDVYDPMI 412
              +  Y H+DFI +M A ++VY+ MI
Sbjct: 395 HKEILAYNHLDFIWAMDAPQEVYNEMI 389

BLAST of Clc02G06880 vs. ExPASy Swiss-Prot
Match: Q9Z0M5 (Lysosomal acid lipase/cholesteryl ester hydrolase OS=Mus musculus OX=10090 GN=Lipa PE=1 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.7e-46
Identity = 128/392 (32.65%), Postives = 197/392 (50.26%), Query Frame = 0

Query: 37  SQLVQPAGYPCAEHTIETKDGFLLGLQRV-SSRDEDLAKQKGPPILLLHGLFMGGDGWFL 96
           ++++   GYP  EH++ T DG++L + R+   R     K   P + L HGL      W  
Sbjct: 36  TEIIMRWGYPGEEHSVLTGDGYILSIHRIPRGRKNHFGKGPRPVVYLQHGLLADSSNWVT 95

Query: 97  NSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMIN 156
           N    SLGF+LAD GFDVW+GN RG  WS  H +LS  + EFW +S++E+A YDL   IN
Sbjct: 96  NIDNSSLGFLLADAGFDVWMGNSRGNTWSLKHKTLSVSQDEFWAFSFDEMAKYDLPASIN 155

Query: 157 YIHSLTNR-KIYVVGHSQVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQ-PDIAK 216
           YI + T + +IY VGHSQ                         G  + F A +Q P++AK
Sbjct: 156 YILNKTGQEQIYYVGHSQ-------------------------GCTIGFIAFSQMPELAK 215

Query: 217 KVEAAALLSPISYLEHITAPLVRL--MVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCD 276
           K++   +L+P+  L   + PL++L  + D  L  +     F   +    W ++   ++C 
Sbjct: 216 KIKMFLVLAPVLSLNFASGPLLQLGRLPDPLLKDMFGQKQFLPQSAMLKWLSI---HVCT 275

Query: 277 RLVN---CINVLSSITGEN-CCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYD 336
            ++    C NV   + G N   LN SR D++  + P  +S +N+ H  Q+ +      +D
Sbjct: 276 HVIMKELCANVFFLLCGFNEKNLNMSRVDVYTTHCPAGTSVQNMLHWGQVFKYRKLQAFD 335

Query: 337 YGLL-KNLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELSDWTDLEHTIKELKSVPELV 396
           +G   KN   Y Q  PP +++ ++     LW   GG D L+D  D+  TI  L  +P+LV
Sbjct: 336 WGSSEKNYFHYNQSFPPSYNIKNMRLPTALWS--GGRDWLADINDI--TIL-LTQIPKLV 394

Query: 397 YLEN---YGHVDFILSMTAKKDVYDPMIKFFK 416
           Y +N   + H+DFI  + A   +YD +I   K
Sbjct: 396 YHKNIPEWDHLDFIWGLDAPWKLYDEIISLMK 394

BLAST of Clc02G06880 vs. ExPASy Swiss-Prot
Match: Q8K2A6 (Lipase member M OS=Mus musculus OX=10090 GN=Lipm PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.7e-46
Identity = 125/411 (30.41%), Postives = 208/411 (50.61%), Query Frame = 0

Query: 16  PLYSADDSSYRSVALSDKSLCSQLVQPAGYPCAEHTIETKDGFLLGLQRVSSRDEDLAKQ 75
           P  +AD  ++ +V        S++++  GYP  E+ + T+DG++L + R+      L K+
Sbjct: 37  PTKAADPEAFMNV--------SEIIKHKGYPSEEYEVATEDGYILSVNRIPRGQTRLKKE 96

Query: 76  KGPPILLL-HGLFMGGDGWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDE 135
              P++LL HGL      W  N    SLGFILAD GFDVW+GN RG  WS  H +LS D+
Sbjct: 97  GSRPVVLLQHGLLGDASNWISNLPNNSLGFILADAGFDVWMGNSRGNTWSRKHKTLSIDQ 156

Query: 136 KEFWTWSWEELALYDLAEMINYIHSLT-NRKIYVVGHSQVSYLFYEILNLKSYIVALATS 195
            EFW +S++E+A +DL  +IN+I   T  +K+Y VG+SQ                     
Sbjct: 157 DEFWAFSYDEMARFDLPAVINFILQKTGQKKVYYVGYSQ--------------------- 216

Query: 196 SFIFGTIMSFAAL-TQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTVILAAGF 255
               GT M F A  T P++A K++    L+PI+ +++  +P  + ++   +   +L  G 
Sbjct: 217 ----GTTMGFIAFSTMPELAHKIKMYFALAPIATVKYARSPGTKFLLLPDMMIKVL-FGR 276

Query: 256 RELNFKSDWGTVLLDNLCDRLV---NCINVLSSITGENC-CLNRSRFDLFFEYEPHPSSA 315
           +E  +++ +   L   LC +++    C N++  + G N   +N SR +++  + P  +S 
Sbjct: 277 QEFLYQTRFFRQLFIYLCGQMILDQICSNIILLLGGFNTNNMNMSRANVYVAHTPAGTSV 336

Query: 316 KNLHHLFQMIRKGTFSRYDYGL-LKNLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELS 375
           +N+ H  Q +  G    +D+G   KN     Q  P  + +  +     +W   GG D LS
Sbjct: 337 QNILHWSQAVNSGELRAFDWGSETKNQEKCNQPTPIRYKVRDMMVPTAMWT--GGQDWLS 396

Query: 376 DWTDLEHTIKELKSVPELVYLEN---YGHVDFILSMTAKKDVYDPMIKFFK 416
           +  D++  + E   V  L+Y +N   + HVDFI  + A + VY+ +I   K
Sbjct: 397 NPDDVKTLLSE---VTNLIYHKNIPEWAHVDFIWGLDAPQRVYNEIIHLMK 408

BLAST of Clc02G06880 vs. ExPASy TrEMBL
Match: A0A5A7SJL6 (Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00890 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.5e-207
Identity = 364/432 (84.26%), Postives = 382/432 (88.43%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV--------ALSD-KSLCSQLVQPAGYPCAEHT 60
           M TPLMAL+FL+YFFPL+SA DSS  +V        ALSD KSLCSQLV PAGYPCAEH 
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGF 120
           I+TKDGFLLGLQRVSSR+ DL KQKGPPILLLHGLFM GDGWFLNSARQSLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHS 180
           DVWIGNVRGTRWSHGH+SLSEDEKEFW WSWEELALYDLAEMINYI+SLTNRKIY+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHIT 240
           Q                         GTIMSFAALTQP+IAK VEAAALLSPISYLEHIT
Sbjct: 181 Q-------------------------GTIMSFAALTQPEIAKNVEAAALLSPISYLEHIT 240

Query: 241 APLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN 300
           APLVRLMVDTHLDT+ILA+GF ELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN
Sbjct: 241 APLVRLMVDTHLDTIILASGFHELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN 300

Query: 301 RSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIP 360
           RSRFDLFFEYEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKNLRVYGQR PPEFDLS IP
Sbjct: 301 RSRFDLFFEYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRMPPEFDLSLIP 360

Query: 361 KSLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMI 420
           +SLPLWMAYGGNDELSDWTDLEHTIK++KSVPELVYLENYGHVDFILSM AK+DVYDPMI
Sbjct: 361 ESLPLWMAYGGNDELSDWTDLEHTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMI 407

Query: 421 KFFKSLGKSSSL 424
           KFFKSLGKSSSL
Sbjct: 421 KFFKSLGKSSSL 407

BLAST of Clc02G06880 vs. ExPASy TrEMBL
Match: A0A1S3C115 (Lipase OS=Cucumis melo OX=3656 GN=LOC103495266 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.5e-207
Identity = 364/432 (84.26%), Postives = 382/432 (88.43%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV--------ALSD-KSLCSQLVQPAGYPCAEHT 60
           M TPLMAL+FL+YFFPL+SA DSS  +V        ALSD KSLCSQLV PAGYPCAEH 
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGF 120
           I+TKDGFLLGLQRVSSR+ DL KQKGPPILLLHGLFM GDGWFLNSARQSLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHS 180
           DVWIGNVRGTRWSHGH+SLSEDEKEFW WSWEELALYDLAEMINYI+SLTNRKIY+VGHS
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 QVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHIT 240
           Q                         GTIMSFAALTQP+IAK VEAAALLSPISYLEHIT
Sbjct: 181 Q-------------------------GTIMSFAALTQPEIAKNVEAAALLSPISYLEHIT 240

Query: 241 APLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN 300
           APLVRLMVDTHLDT+ILA+GF ELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN
Sbjct: 241 APLVRLMVDTHLDTIILASGFHELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN 300

Query: 301 RSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIP 360
           RSRFDLFFEYEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKNLRVYGQR PPEFDLS IP
Sbjct: 301 RSRFDLFFEYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRMPPEFDLSLIP 360

Query: 361 KSLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMI 420
           +SLPLWMAYGGNDELSDWTDLEHTIK++KSVPELVYLENYGHVDFILSM AK+DVYDPMI
Sbjct: 361 ESLPLWMAYGGNDELSDWTDLEHTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMI 407

Query: 421 KFFKSLGKSSSL 424
           KFFKSLGKSSSL
Sbjct: 421 KFFKSLGKSSSL 407

BLAST of Clc02G06880 vs. ExPASy TrEMBL
Match: A0A6J1GLC0 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.5e-205
Identity = 362/431 (83.99%), Postives = 378/431 (87.70%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV-------ALSD-KSLCSQLVQPAGYPCAEHTI 60
           M  PLMA++FLL FF L+SADDSSYRS+        LSD KSLCSQLVQPAGYPCAEH I
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  ETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFD 120
           +TKDGFLLGLQRVSS D DL KQKGPPILLLHGLFMGGD WFLNSAR+SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQ 180
           VWIGNVRGTRWSHGHTSLSEDEKEFW WSWEELALYDLA MI+YI+SLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 VSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240
                                    GTIMSFAALTQPDIA+KVEAAALLSPISYLEHITA
Sbjct: 181 -------------------------GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITA 240

Query: 241 PLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNR 300
           PLVRLMVDTHLDT+ILAAGF ELNFKSDWGTVLLD+LCDRLVNCINVLSSITGENCCLN 
Sbjct: 241 PLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNG 300

Query: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPK 360
           SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPP FDLS IPK
Sbjct: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPK 360

Query: 361 SLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIK 420
           SLPLWMAYGG+DELSDWTDL+HTIKELKSVPELVYLENYGHVDFILSM AK+DVYDPMIK
Sbjct: 361 SLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIK 406

Query: 421 FFKSLGKSSSL 424
           F KSLGKS +L
Sbjct: 421 FLKSLGKSGNL 406

BLAST of Clc02G06880 vs. ExPASy TrEMBL
Match: A0A0A0K4L2 (Lipase OS=Cucumis sativus OX=3659 GN=Csa_7G072760 PE=3 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.1e-204
Identity = 359/432 (83.10%), Postives = 381/432 (88.19%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV--------ALSD-KSLCSQLVQPAGYPCAEHT 60
           M  PL+A IFLL+FFPL+SA DSS+ SV        ALSD KSLCSQLV PAGYPCAEH 
Sbjct: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60

Query: 61  IETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGF 120
           I+TKDGFLLGLQRVSSRD +L KQKGPPILLLHGLFM GDGWFLNSARQSLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHS 180
           DVWIGNVRGTRWS+GH+SLSEDEKEFW WSWEELALYDLAEMINYI+SLTN+KIY+VGHS
Sbjct: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180

Query: 181 QVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHIT 240
           Q                         GTIMSFAALTQPDIAKKV AAALLSPISYLEHIT
Sbjct: 181 Q-------------------------GTIMSFAALTQPDIAKKVGAAALLSPISYLEHIT 240

Query: 241 APLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLN 300
           APLVRLMVDTHLDT+ILA+GF ELNFKSDWGTVLLDNLCDRLVNCIN+LSSITGENCCLN
Sbjct: 241 APLVRLMVDTHLDTIILASGFHELNFKSDWGTVLLDNLCDRLVNCINILSSITGENCCLN 300

Query: 301 RSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIP 360
           RSRFDLFF+YEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKNLRVYGQR PPEFDLS IP
Sbjct: 301 RSRFDLFFKYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIP 360

Query: 361 KSLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMI 420
           +SLPLWMAYGGNDELSDWTDLE+TIK++KSVPELVYLENYGHVDFILSM AK+DVYDPMI
Sbjct: 361 ESLPLWMAYGGNDELSDWTDLENTIKKVKSVPELVYLENYGHVDFILSMKAKEDVYDPMI 407

Query: 421 KFFKSLGKSSSL 424
           KFFKSLGKSSSL
Sbjct: 421 KFFKSLGKSSSL 407

BLAST of Clc02G06880 vs. ExPASy TrEMBL
Match: A0A6J1GL56 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.1e-204
Identity = 361/428 (84.35%), Postives = 376/428 (87.85%), Query Frame = 0

Query: 1   MTTPLMALIFLLYFFPLYSADDSSYRSV-------ALSD-KSLCSQLVQPAGYPCAEHTI 60
           M  PLMA++FLL FF L+SADDSSYRS+        LSD KSLCSQLVQPAGYPCAEH I
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  ETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFD 120
           +TKDGFLLGLQRVSS D DL KQKGPPILLLHGLFMGGD WFLNSAR+SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQ 180
           VWIGNVRGTRWSHGHTSLSEDEKEFW WSWEELALYDLA MI+YI+SLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 VSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITA 240
                                    GTIMSFAALTQPDIA+KVEAAALLSPISYLEHITA
Sbjct: 181 -------------------------GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITA 240

Query: 241 PLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNR 300
           PLVRLMVDTHLDT+ILAAGF ELNFKSDWGTVLLD+LCDRLVNCINVLSSITGENCCLN 
Sbjct: 241 PLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNG 300

Query: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPK 360
           SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPP FDLS IPK
Sbjct: 301 SRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPK 360

Query: 361 SLPLWMAYGGNDELSDWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIK 420
           SLPLWMAYGG+DELSDWTDL+HTIKELKSVPELVYLENYGHVDFILSM AK+DVYDPMIK
Sbjct: 361 SLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIK 403

BLAST of Clc02G06880 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 495.0 bits (1273), Expect = 6.1e-140
Identity = 237/415 (57.11%), Postives = 305/415 (73.49%), Query Frame = 0

Query: 8   LIFLLYFFPLYSADDSSYRSVALSDKSLCSQLVQPAGYPCAEHTIETKDGFLLGLQRVSS 67
           L+ +L    ++SA   S+        SLC+ L+ PA Y C EH+I+TKDG++L LQRV+S
Sbjct: 5   LVAVLTSLTIFSALTQSHLLHGSPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVAS 64

Query: 68  RDEDLAKQKGPPILLLHGLFMGGDGWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGH 127
               L  Q GPP+LL HGLFM GD WFLNS ++SLGFILAD+GFDVW+GNVRGTR+S+GH
Sbjct: 65  LGPRL--QSGPPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGH 124

Query: 128 TSLSEDEKEFWTWSWEELALYDLAEMINYIHSLTNRKIYVVGHSQVSYLFYEILNLKSYI 187
            +LS+ +KEFW WSW++LA+YDLAEMI Y++S++N KI++VGHSQ               
Sbjct: 125 VTLSDTDKEFWDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQ--------------- 184

Query: 188 VALATSSFIFGTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTVI 247
                     GTIMSFAALTQP +A+ VEAAALL PISYL+H+TAPLV  MV  HLD ++
Sbjct: 185 ----------GTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPLVERMVFMHLDQMV 244

Query: 248 LAAGFRELNFKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNRSRFDLFFEYEPHPSS 307
           +A G  ++NF+SD    L+D+LC+  ++C + L+SITG NCC N S+ + + +YEPHPSS
Sbjct: 245 VALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASKIEYYLDYEPHPSS 304

Query: 308 AKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELS 367
            KN+ HLFQMIRKGTF++YDYG  KNLR YG  KPPEF LSHIP SLP+WM YGG D L+
Sbjct: 305 VKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASLPMWMGYGGTDGLA 364

Query: 368 DWTDLEHTIKELKSVPELVYLENYGHVDFILSMTAKKDVYDPMIKFFKSLGKSSS 423
           D TD+EHT+ EL S PEL+YLE+YGH+DF+L  +AK+DVY  MI+FF++  KSSS
Sbjct: 365 DVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFFRAKVKSSS 392

BLAST of Clc02G06880 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 280.8 bits (717), Expect = 1.8e-75
Identity = 146/390 (37.44%), Postives = 222/390 (56.92%), Query Frame = 0

Query: 35  LCSQLVQPAGYPCAEHTIETKDGFLLGLQRV-SSRDEDLAKQKG--PPILLLHGLFMGGD 94
           +C+  V   GY C EH + T+DG++L +QR+   R   +A   G   P+L+ HG+ + G 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGM 110

Query: 95  GWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDLA 154
            W LN A Q+L  ILAD GFDVW+GN RGTR+S  H  L+  ++ FW W+W+EL  YDL 
Sbjct: 111 SWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLP 170

Query: 155 EMINYIHSLTNRKIYVVGHSQVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPDI 214
            M ++IH LT +KI+ +GHS                          GT++ FA+ ++  +
Sbjct: 171 AMFDHIHGLTGQKIHYLGHS-------------------------LGTLIGFASFSEKGL 230

Query: 215 AKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDNLCD 274
             +V +AA+LSP++YL H+T  +  +   T L       G+ E N KS      +  +C 
Sbjct: 231 VDQVRSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICL 290

Query: 275 RL-VNCINVLSSITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGL 334
           +  ++C +++S ITG+NCCLN S  DLF   EP  +S KN+ HL Q +R     +Y+YG 
Sbjct: 291 KAGIDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGS 350

Query: 335 L-KNLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELSDWTDLEHTIKELK----SVPEL 394
             +N++ YGQ  PP +++S IP  LPL+ +YGG D L+D  D+E  + + K        +
Sbjct: 351 SDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNV 410

Query: 395 VYLENYGHVDFILSMTAKKDVYDPMIKFFK 416
            ++++Y H DFI+ +TAK  VY+ +  FFK
Sbjct: 411 QFVKDYAHADFIMGVTAKDVVYNQVATFFK 415

BLAST of Clc02G06880 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 5.6e-16
Identity = 91/383 (23.76%), Postives = 149/383 (38.90%), Query Frame = 0

Query: 31  SDKSLCSQLVQPAGYPCAEHTIETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGG 90
           +D   C  ++   GYP     + T DG++L L+R+  RD   A      + L HG+    
Sbjct: 291 TDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIPRRDARKA------VFLQHGVLDSS 350

Query: 91  DGWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDL 150
            GW  N    S  F   D G+DV++GN RG   S  H + +   KEFW +S  E    D+
Sbjct: 351 MGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDI 410

Query: 151 AEMINYIHSLTNRKIYVVGHSQVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPD 210
             MI  IH +   ++ +   +       E +N +      A    + G  +    +T+  
Sbjct: 411 PAMIEKIHEIKTTELKLCQPN-----IDEEINQEEPYKLCAICHSLGGAAILMYVITRKI 470

Query: 211 IAK--KVEAAALLSPISYLEHITAPLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDN 270
             K  ++    LLSP  + E        +       + +LA         + +  +LL+ 
Sbjct: 471 KEKPHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNK 530

Query: 271 LCDRLVN-------CINVLSSITGENCCLNRSRFDL-FFEYEPHPS-SAKNLHHLFQMIR 330
           L     N          ++S + G +         L  +     P+ S +   HL Q+  
Sbjct: 531 LARDFHNYPALGGLVQTLMSYVVGGDSSNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKH 590

Query: 331 KGTFSRYDYGLLK-NLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDEL--SDWTDLEHTI 390
            G F  YDYG    N+ VYG  +P +   S+    +P+ +  G ND++  S      + +
Sbjct: 591 TGKFRMYDYGSRSANMEVYGSPEPLDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNV 650

Query: 391 KELKSVPELVYLENYGHVDFILS 400
                V        Y H+DF  S
Sbjct: 651 MRDAEVDVSFNEFEYAHLDFTFS 661

BLAST of Clc02G06880 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 5.6e-16
Identity = 91/383 (23.76%), Postives = 149/383 (38.90%), Query Frame = 0

Query: 31  SDKSLCSQLVQPAGYPCAEHTIETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGG 90
           +D   C  ++   GYP     + T DG++L L+R+  RD   A      + L HG+    
Sbjct: 222 TDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIPRRDARKA------VFLQHGVLDSS 281

Query: 91  DGWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDL 150
            GW  N    S  F   D G+DV++GN RG   S  H + +   KEFW +S  E    D+
Sbjct: 282 MGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDI 341

Query: 151 AEMINYIHSLTNRKIYVVGHSQVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPD 210
             MI  IH +   ++ +   +       E +N +      A    + G  +    +T+  
Sbjct: 342 PAMIEKIHEIKTTELKLCQPN-----IDEEINQEEPYKLCAICHSLGGAAILMYVITRKI 401

Query: 211 IAK--KVEAAALLSPISYLEHITAPLVRLMVDTHLDTVILAAGFRELNFKSDWGTVLLDN 270
             K  ++    LLSP  + E        +       + +LA         + +  +LL+ 
Sbjct: 402 KEKPHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNK 461

Query: 271 LCDRLVN-------CINVLSSITGENCCLNRSRFDL-FFEYEPHPS-SAKNLHHLFQMIR 330
           L     N          ++S + G +         L  +     P+ S +   HL Q+  
Sbjct: 462 LARDFHNYPALGGLVQTLMSYVVGGDSSNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKH 521

Query: 331 KGTFSRYDYGLLK-NLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDEL--SDWTDLEHTI 390
            G F  YDYG    N+ VYG  +P +   S+    +P+ +  G ND++  S      + +
Sbjct: 522 TGKFRMYDYGSRSANMEVYGSPEPLDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNV 581

Query: 391 KELKSVPELVYLENYGHVDFILS 400
                V        Y H+DF  S
Sbjct: 582 MRDAEVDVSFNEFEYAHLDFTFS 592

BLAST of Clc02G06880 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 2.6e-13
Identity = 91/384 (23.70%), Postives = 154/384 (40.10%), Query Frame = 0

Query: 31  SDKSLCSQLVQPAGYPCAEHTIETKDGFLLGLQRVSSRDEDLAKQKGPPILLLHGLFMGG 90
           +D   C  ++   GYP     + T DG+ L L+R+  RD   A      + L HG+    
Sbjct: 289 TDTRTCQDVITELGYPYEAIRVVTSDGYGLLLERIPRRDARKA------VYLQHGVMDSS 348

Query: 91  DGWFLNSARQSLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKEFWTWSWEELALYDL 150
            GW  N    S  F   D G+DV++GN RG   S  H   +   K+FW +S  E A  D+
Sbjct: 349 MGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVKKNISSKDFWRYSINEHATEDI 408

Query: 151 AEMINYIHSLTNRKIYVVGHSQVSYLFYEILNLKSYIVALATSSFIFGTIMSFAALTQPD 210
             MI  IH +   ++ +   +       E++N          S  + G  +    +T+  
Sbjct: 409 PAMIEKIHEIKTSELKLYQPT-----MEEVVNEDQPYKLCVVSHSLGGAAVLMYVITRKI 468

Query: 211 IAK--KVEAAALLSPISYLEHITAPLVRLMVDTHLDT-VILAAGFRELNFKSDWGTVLLD 270
             K  ++    LLSP  +  + +     LM  T L    +L+         + +  +LL+
Sbjct: 469 EEKPHRLSRLILLSPAGF-HYDSNMCFTLMEYTFLFLGPVLSRIVPAFYIPTKFFRMLLN 528

Query: 271 NLCDRLVN-------CINVLSSITGENCCLNRSRFDL-FFEYEPHPS-SAKNLHHLFQMI 330
            L     N          ++S + G +         L  +     P  S +   HL Q+ 
Sbjct: 529 KLARDFHNYPAVGGLVQTLMSYVVGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQIK 588

Query: 331 RKGTFSRYDYGLLK-NLRVYGQRKPPEFDLSHIPKSLPLWMAYGGNDELSDWTDLEHTIK 390
             G F  +DYG    N+ VYG  +P +    +    +P+ +  G  D++   + +    +
Sbjct: 589 HSGKFKMFDYGSSSANMDVYGSPEPLDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKHYR 648

Query: 391 ELK-SVPELVYLE-NYGHVDFILS 400
            ++ S  ++ Y E  Y H+DF  S
Sbjct: 649 VMRDSGVDVSYNEFEYAHLDFTFS 659

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886839.12.4e-21085.15triacylglycerol lipase 1 isoform X1 [Benincasa hispida][more]
XP_008454984.19.3e-20784.26PREDICTED: triacylglycerol lipase 1 isoform X1 [Cucumis melo] >KAA0031334.1 tria... [more]
XP_022952782.11.1e-20483.99triacylglycerol lipase 1 isoform X2 [Cucurbita moschata][more]
XP_023553816.11.5e-20484.35triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023553815.11.5e-20484.35triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q71DJ58.6e-13957.11Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
Q67ZU12.6e-7437.44Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1[more]
P046347.5e-5032.56Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1[more]
Q9Z0M51.7e-4632.65Lysosomal acid lipase/cholesteryl ester hydrolase OS=Mus musculus OX=10090 GN=Li... [more]
Q8K2A61.7e-4630.41Lipase member M OS=Mus musculus OX=10090 GN=Lipm PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SJL64.5e-20784.26Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00890 PE=3 SV=... [more]
A0A1S3C1154.5e-20784.26Lipase OS=Cucumis melo OX=3656 GN=LOC103495266 PE=3 SV=1[more]
A0A6J1GLC05.5e-20583.99Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
A0A0A0K4L22.1e-20483.10Lipase OS=Cucumis sativus OX=3659 GN=Csa_7G072760 PE=3 SV=1[more]
A0A6J1GL562.1e-20484.35Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G15230.16.1e-14057.11lipase 1 [more]
AT5G14180.11.8e-7537.44Myzus persicae-induced lipase 1 [more]
AT1G73920.15.6e-1623.76alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.25.6e-1623.76alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.12.6e-1323.70alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 37..96
e-value: 3.3E-16
score: 58.6
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 26..416
e-value: 1.5E-108
score: 365.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 37..415
IPR025483Lipase, eukaryoticPIRSFPIRSF000862Steryl_ester_lipcoord: 190..419
e-value: 1.5E-58
score: 196.5
coord: 2..196
e-value: 5.3E-61
score: 204.6
NoneNo IPR availablePANTHERPTHR11005:SF118TRIACYLGLYCEROL LIPASE 1coord: 26..417
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 26..417

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc02G06880.2Clc02G06880.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:2000033 regulation of seed dormancy process
biological_process GO:0010029 regulation of seed germination
biological_process GO:0009739 response to gibberellin
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004806 triglyceride lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds