Clc02G04153 (gene) Watermelon (cordophanus) v2

Overview
NameClc02G04153
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description(RS)-norcoclaurine 6-O-methyltransferase-like
LocationClcChr02: 3541396 .. 3541762 (+)
RNA-Seq ExpressionClc02G04153
SyntenyClc02G04153
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGAAATTGATAGCAAAGAGTCCAATAAATTGAAAATAGAAGAAGAGGATGATATCAAAGTCTAAGTATGGAAATACATATTAGGTGTTGTTGAAATGGCAGTAATTTAAATGTGGCATTGAGCTCAAAATAGGAGACGTCAATGAAAGCCAAGGAGATTTCATGACACTCTCCCAGCTATCTTCTTCTTTGAAGTGTTGTCCTTTGCTTCTACCCCGCATCGTGCGGTTCCTTGTTCTTCGAGGCATTTTCAAAGAAGAAAAAACCACACAAAGCTTACTCTCAAACGCCCATGTCTCGGCTCCTCCTATCACTGGACCAAATAGTTTGGCTCCCTTACATCTACTTGAGTTCATGTCTTAA

mRNA sequence

ATGGATGAAATTGATAGCAAAGAGTCCAATAAATTGAAAATAGAAGAAGAGGATGATATCAAACTCAAAATAGGAGACGTCAATGAAAGCCAAGGAGATTTCATGACACTCTCCCAGCTATCTTCTTCTTTGAAGTGTTGTCCTTTGCTTCTACCCCGCATCGTGCGGTTCCTTGTTCTTCGAGGCATTTTCAAAGAAGAAAAAACCACACAAAGCTTACTCTCAAACGCCCATGTCTCGGCTCCTCCTATCACTGGACCAAATAGTTTGGCTCCCTTACATCTACTTGAGTTCATGTCTTAA

Coding sequence (CDS)

ATGGATGAAATTGATAGCAAAGAGTCCAATAAATTGAAAATAGAAGAAGAGGATGATATCAAACTCAAAATAGGAGACGTCAATGAAAGCCAAGGAGATTTCATGACACTCTCCCAGCTATCTTCTTCTTTGAAGTGTTGTCCTTTGCTTCTACCCCGCATCGTGCGGTTCCTTGTTCTTCGAGGCATTTTCAAAGAAGAAAAAACCACACAAAGCTTACTCTCAAACGCCCATGTCTCGGCTCCTCCTATCACTGGACCAAATAGTTTGGCTCCCTTACATCTACTTGAGTTCATGTCTTAA

Protein sequence

MDEIDSKESNKLKIEEEDDIKLKIGDVNESQGDFMTLSQLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHVSAPPITGPNSLAPLHLLEFMS
Homology
BLAST of Clc02G04153 vs. NCBI nr
Match: XP_004144871.1 (acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical protein Csa_020497 [Cucumis sativus])

HSP 1 Score: 108.2 bits (269), Expect = 4.1e-20
Identity = 68/119 (57.14%), Postives = 73/119 (61.34%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD----------------------IKLKIGDVNESQGDFMTLS 60
           MD+  SK SNKLKIEEED                       I+LKIGDV ES GD MTLS
Sbjct: 1   MDDTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLS 60

Query: 61  QLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHVSAPPITGPNSLAPLHLLE 98
           QLSSSL C PLLL RI+RFLV RGIFKEEKTT+             TGPNS+APLHLLE
Sbjct: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTKCYSHTPMSRLLTTTGPNSMAPLHLLE 119

BLAST of Clc02G04153 vs. NCBI nr
Match: XP_008447905.1 (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 105.1 bits (261), Expect = 3.5e-19
Identity = 65/119 (54.62%), Postives = 75/119 (63.03%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD----------------------IKLKIGDVNESQGDFMTLS 60
           MD+  +KESNKLKIEEE+                       I+LKIGDV ES GD MTLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60

Query: 61  QLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHVSAPPITGPNSLAPLHLLE 98
           QLSSSL C PLLL RI+RFLV RGIFKEEK+++S            TGPNS+AP+HLLE
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLE 119

BLAST of Clc02G04153 vs. NCBI nr
Match: KAA0040348.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23356.1 (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 103.6 bits (257), Expect = 1.0e-18
Identity = 64/119 (53.78%), Postives = 75/119 (63.03%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD----------------------IKLKIGDVNESQGDFMTLS 60
           MD+  +KESNKLKIEEE+                       I+LKIGDV ES GD +TLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLS 60

Query: 61  QLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHVSAPPITGPNSLAPLHLLE 98
           QLSSSL C PLLL RI+RFLV RGIFKEEK+++S            TGPNS+AP+HLLE
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLE 119

BLAST of Clc02G04153 vs. NCBI nr
Match: XP_038888743.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 101.3 bits (251), Expect = 5.0e-18
Identity = 65/119 (54.62%), Postives = 71/119 (59.66%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD----------------------IKLKIGDVNESQGDFMTLS 60
           MDE  SKESNKLKIEEE+                       I+LKIGD  ES G  MTLS
Sbjct: 1   MDENGSKESNKLKIEEEESDLKVEVWKYIFGFVEMAVIKCAIELKIGDTIESNGGSMTLS 60

Query: 61  QLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHVSAPPITGPNSLAPLHLLE 98
           +LSSSL   PLLL R++RFLV RGIFKEEK TQS            TGPNSLAP+HLLE
Sbjct: 61  ELSSSLNSSPLLLHRVLRFLVHRGIFKEEKATQSYSQTPMSRLLTTTGPNSLAPIHLLE 119

BLAST of Clc02G04153 vs. NCBI nr
Match: KAG6572137.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 81.3 bits (199), Expect = 5.4e-12
Identity = 58/123 (47.15%), Postives = 73/123 (59.35%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD-----------------------IKLKIGDVNESQGDFMTL 60
           MD I SK+S KLKIEEE++                       I+LKIGD  E+ G  MTL
Sbjct: 1   MDNIGSKDS-KLKIEEEENNDIKIQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTL 60

Query: 61  SQLSSSLKCCPLLLPRIVRFLVLRGIFKEEKT---TQSLLSNAHVSAPPITGPNSLAPLH 98
           ++LSSSL C PLLL RI+RFL+ RGIFKEE T    Q+ +S    S    +G +SLAP+H
Sbjct: 61  TELSSSLNCSPLLLHRILRFLIHRGIFKEEPTKSYAQTPMSRLLTS----SGAHSLAPMH 118

BLAST of Clc02G04153 vs. ExPASy TrEMBL
Match: A0A0A0K0Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.0e-20
Identity = 68/119 (57.14%), Postives = 73/119 (61.34%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD----------------------IKLKIGDVNESQGDFMTLS 60
           MD+  SK SNKLKIEEED                       I+LKIGDV ES GD MTLS
Sbjct: 1   MDDTSSKASNKLKIEEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLS 60

Query: 61  QLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHVSAPPITGPNSLAPLHLLE 98
           QLSSSL C PLLL RI+RFLV RGIFKEEKTT+             TGPNS+APLHLLE
Sbjct: 61  QLSSSLNCSPLLLHRILRFLVHRGIFKEEKTTKCYSHTPMSRLLTTTGPNSMAPLHLLE 119

BLAST of Clc02G04153 vs. ExPASy TrEMBL
Match: A0A1S3BHY1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490251 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-19
Identity = 65/119 (54.62%), Postives = 75/119 (63.03%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD----------------------IKLKIGDVNESQGDFMTLS 60
           MD+  +KESNKLKIEEE+                       I+LKIGDV ES GD MTLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLS 60

Query: 61  QLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHVSAPPITGPNSLAPLHLLE 98
           QLSSSL C PLLL RI+RFLV RGIFKEEK+++S            TGPNS+AP+HLLE
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLE 119

BLAST of Clc02G04153 vs. ExPASy TrEMBL
Match: A0A5D3DI94 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004200 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.9e-19
Identity = 64/119 (53.78%), Postives = 75/119 (63.03%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD----------------------IKLKIGDVNESQGDFMTLS 60
           MD+  +KESNKLKIEEE+                       I+LKIGDV ES GD +TLS
Sbjct: 1   MDDTGNKESNKLKIEEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLS 60

Query: 61  QLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHVSAPPITGPNSLAPLHLLE 98
           QLSSSL C PLLL RI+RFLV RGIFKEEK+++S            TGPNS+AP+HLLE
Sbjct: 61  QLSSSLSCSPLLLHRILRFLVHRGIFKEEKSSKSYSHTPMSRLLTTTGPNSMAPIHLLE 119

BLAST of Clc02G04153 vs. ExPASy TrEMBL
Match: A0A6J1GLV0 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455162 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.4e-12
Identity = 58/123 (47.15%), Postives = 72/123 (58.54%), Query Frame = 0

Query: 1   MDEIDSKESNKLKIEEEDD-----------------------IKLKIGDVNESQGDFMTL 60
           MD I SK+S KLKIEEE++                       I+LKIGD  E+ G  MTL
Sbjct: 1   MDNIGSKDS-KLKIEEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTL 60

Query: 61  SQLSSSLKCCPLLLPRIVRFLVLRGIFKEEKT---TQSLLSNAHVSAPPITGPNSLAPLH 98
           + LSSSL C PLLL RI+RFL+ RGIFKEE T    Q+ +S    S    +G +SLAP+H
Sbjct: 61  TDLSSSLNCSPLLLHRILRFLIHRGIFKEEPTKSYAQTPMSRLLTS----SGAHSLAPMH 118

BLAST of Clc02G04153 vs. ExPASy TrEMBL
Match: A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.1e-10
Identity = 46/81 (56.79%), Postives = 52/81 (64.20%), Query Frame = 0

Query: 20  IKLKIGDVNESQGDFMTLSQLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAHV 79
           I+LKI D  ES G  MTLSQLSS+L C P LL RI+RFL  RGIFKEE T Q+L   A  
Sbjct: 40  IELKIADTIESHGSPMTLSQLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQT 99

Query: 80  SAPPI---TGPNSLAPLHLLE 98
               +    G NS+APL LLE
Sbjct: 100 PLSRLLASNGNNSMAPLLLLE 120

BLAST of Clc02G04153 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 41.6 bits (96), Expect = 4.4e-04
Identity = 25/64 (39.06%), Postives = 37/64 (57.81%), Query Frame = 0

Query: 19  DIKLKIGDVNESQGDFMTLSQLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSLLSNAH 78
           D+K+     N      +TL++LSS++   P  L RI+RFLV +GIFKE  T    L+  +
Sbjct: 51  DLKIPEAIENHPSSQPVTLAELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDG-LATGY 110

Query: 79  VSAP 83
           V+ P
Sbjct: 111 VNTP 113

BLAST of Clc02G04153 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 41.2 bits (95), Expect = 5.7e-04
Identity = 23/55 (41.82%), Postives = 32/55 (58.18%), Query Frame = 0

Query: 19 DIKLKIGDVNESQGDFMTLSQLSSSLKCCPLLLPRIVRFLVLRGIFKEEKTTQSL 74
          D+K+     N      +TLS+LSS++   P  L RI+RFLV +G+FKE  T   L
Sbjct: 37 DLKIPEAIENHPSSQPVTLSELSSAVSASPSHLRRIMRFLVHQGLFKEVPTKDGL 91

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004144871.14.1e-2057.14acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical p... [more]
XP_008447905.13.5e-1954.62PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo][more]
KAA0040348.11.0e-1853.78(RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK233... [more]
XP_038888743.15.0e-1854.62acetylserotonin O-methyltransferase-like [Benincasa hispida][more]
KAG6572137.15.4e-1247.15Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. soro... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0K0Q22.0e-2057.14Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1[more]
A0A1S3BHY11.7e-1954.62(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5D3DI944.9e-1953.78(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1GLV04.4e-1247.15xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1C2I81.1e-1056.793'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica ch... [more]
Match NameE-valueIdentityDescription
AT4G35160.14.4e-0439.06O-methyltransferase family protein [more]
AT4G35150.15.7e-0441.82O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..81
e-value: 4.1E-8
score: 34.8
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 20..81

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc02G04153.1Clc02G04153.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008168 methyltransferase activity