Clc02G03070 (gene) Watermelon (cordophanus) v2

BLAST of Clc02G03070 vs. NCBI nr
Match: XP_016902818.1 (PREDICTED: long chain acyl-CoA synthetase 8 isoform X1 [Cucumis melo])

HSP 1 Score: 1378.6 bits (3567), Expect = 0.0e+00
Identity = 691/805 (85.84%), Postives = 721/805 (89.57%), Query Frame = 0

Query: 103 VSYCYRSTAPASPLVYVCFELVDHLLEKQSCDVSVIMKFEDAQFVIDSIAYTFMTGDSIE 162
           VSYCYRSTAP SPL  +CF+                              +TFMTG+SIE
Sbjct: 2   VSYCYRSTAPPSPLFDLCFK-----------------------------GHTFMTGESIE 61

Query: 163 SIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTD 222
           SIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+KRRK RGIP D
Sbjct: 62  SIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVD 121

Query: 223 AGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASD 282
            GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISKEFVT SD
Sbjct: 122 VGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISKEFVTTSD 181

Query: 283 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCI 342
           GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCAEWF+AFQGCI
Sbjct: 182 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWFVAFQGCI 241

Query: 343 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAG 402
           RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLM ISSSLTT+ NIIYFEDAG
Sbjct: 242 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNIIYFEDAG 301

Query: 403 YETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRG 462
           YETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG PK     
Sbjct: 302 YETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGFPK----- 361

Query: 463 GDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAET 522
                          GVMISH NIVAT AGV TVIPKL+ DDVYLAYLPLAHVLELAAE 
Sbjct: 362 ---------------GVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHVLELAAEA 421

Query: 523 VILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKKVEE 582
           VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRVRDGVLKKVEE
Sbjct: 422 VILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEE 481

Query: 583 KGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAP 642
           KGGLVKKLFHI YNRQLA LEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR+RF+LCGGAP
Sbjct: 482 KGGLVKKLFHIAYNRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAP 541

Query: 643 LSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG 702
           LSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG
Sbjct: 542 LSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG 601

Query: 703 GYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCL 762
           GYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+EVYKDDEKG RWFYTGDIGAFHPDGCL
Sbjct: 602 GYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCL 661

Query: 763 EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALEQ 822
           EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALE+
Sbjct: 662 EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEE 721

Query: 823 WAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESGL 882
           WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA DPWTPESGL
Sbjct: 722 WAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGL 757

Query: 883 VTAALKIKREQVKAKFKNELQQLYK 908
           VTAALKIKREQVKAKFK++LQQLYK
Sbjct: 782 VTAALKIKREQVKAKFKDQLQQLYK 757

BLAST of Clc02G03070 vs. NCBI nr
Match: KAA0059212.1 (long chain acyl-CoA synthetase 8 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1375.5 bits (3559), Expect = 0.0e+00
Identity = 692/806 (85.86%), Postives = 722/806 (89.58%), Query Frame = 0

Query: 103 VSYCYRSTAPASPLVYVCFELVDHLLEKQSCDVSVIMKFEDAQFVIDSIAYTFMTGDSIE 162
           VSYCYRSTAP SPL  +CF+                              +TFMTG+SIE
Sbjct: 2   VSYCYRSTAPPSPLFDLCFK-----------------------------GHTFMTGESIE 61

Query: 163 SIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTD 222
           SIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+KRRK RGIP D
Sbjct: 62  SIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVD 121

Query: 223 AGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASD 282
            GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISKEFVT SD
Sbjct: 122 VGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISKEFVTTSD 181

Query: 283 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCI 342
           GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCAEWF+AFQGCI
Sbjct: 182 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWFVAFQGCI 241

Query: 343 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAG 402
           RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLR LM ISSSLTT+ NIIYFEDAG
Sbjct: 242 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMEISSSLTTVVNIIYFEDAG 301

Query: 403 YETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRG 462
           YETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG PK     
Sbjct: 302 YETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGFPK----- 361

Query: 463 GDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAET 522
                          GVMISH NIVAT AGVMTVIPKL+ DDVYLAYLPLAHVLELAAE 
Sbjct: 362 ---------------GVMISHGNIVATVAGVMTVIPKLNSDDVYLAYLPLAHVLELAAEA 421

Query: 523 VILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKK-VE 582
           VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRVRDGVLKK VE
Sbjct: 422 VILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVVE 481

Query: 583 EKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGA 642
           EKGGLVKKLFHI YNRQLAALEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR+RF+LCGGA
Sbjct: 482 EKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGA 541

Query: 643 PLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEE 702
           PLSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEE
Sbjct: 542 PLSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEE 601

Query: 703 GGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGC 762
           GGYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+EVYKDDEKG RWFYTGDIGAFHPDGC
Sbjct: 602 GGYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGC 661

Query: 763 LEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALE 822
           LEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALE
Sbjct: 662 LEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALE 721

Query: 823 QWAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESG 882
           +WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA DPWTPESG
Sbjct: 722 EWAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESG 758

Query: 883 LVTAALKIKREQVKAKFKNELQQLYK 908
           LVTAALKIKREQVKAKFK++LQQLYK
Sbjct: 782 LVTAALKIKREQVKAKFKDQLQQLYK 758

BLAST of Clc02G03070 vs. NCBI nr
Match: TYK19308.1 (long chain acyl-CoA synthetase 8 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1368.6 bits (3541), Expect = 0.0e+00
Identity = 689/805 (85.59%), Postives = 719/805 (89.32%), Query Frame = 0

Query: 103 VSYCYRSTAPASPLVYVCFELVDHLLEKQSCDVSVIMKFEDAQFVIDSIAYTFMTGDSIE 162
           VSYCYRSTAP SPL  +CF+                              +TFMTG+SIE
Sbjct: 2   VSYCYRSTAPPSPLFDLCFK-----------------------------GHTFMTGESIE 61

Query: 163 SIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTD 222
           SIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+KRRK RGIP D
Sbjct: 62  SIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVD 121

Query: 223 AGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASD 282
            GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISKEFVT SD
Sbjct: 122 VGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISKEFVTTSD 181

Query: 283 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCI 342
           GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCAEWF+AFQGCI
Sbjct: 182 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWFVAFQGCI 241

Query: 343 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAG 402
           RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLR LM ISSSLTT+ NIIYFEDAG
Sbjct: 242 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMEISSSLTTVVNIIYFEDAG 301

Query: 403 YETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRG 462
           YETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG PK     
Sbjct: 302 YETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGFPK----- 361

Query: 463 GDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAET 522
                          GVMISH NIVAT AGVMTVIPKL+ DDVYLAYLPLAHVLELAAE 
Sbjct: 362 ---------------GVMISHGNIVATVAGVMTVIPKLNSDDVYLAYLPLAHVLELAAEA 421

Query: 523 VILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKKVEE 582
           VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRVRDGVLKKVEE
Sbjct: 422 VILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEE 481

Query: 583 KGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAP 642
           KGGLVKKLFHI YNRQLAALEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR+RF+LCGGAP
Sbjct: 482 KGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAP 541

Query: 643 LSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG 702
           LSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG
Sbjct: 542 LSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG 601

Query: 703 GYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCL 762
           GYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+E   DDEKG RWFYTGDIGAFHPDGCL
Sbjct: 602 GYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNE---DDEKGTRWFYTGDIGAFHPDGCL 661

Query: 763 EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALEQ 822
           EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALE+
Sbjct: 662 EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEE 721

Query: 823 WAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESGL 882
           WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA DPWTPESGL
Sbjct: 722 WAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGL 754

Query: 883 VTAALKIKREQVKAKFKNELQQLYK 908
           VTAALKIKREQVKAKFK++LQQLYK
Sbjct: 782 VTAALKIKREQVKAKFKDQLQQLYK 754

BLAST of Clc02G03070 vs. NCBI nr
Match: XP_016902819.1 (PREDICTED: long chain acyl-CoA synthetase 8 isoform X2 [Cucumis melo])

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 676/755 (89.54%), Postives = 704/755 (93.25%), Query Frame = 0

Query: 153 YTFMTGDSIESIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRK 212
           +TFMTG+SIESIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+K
Sbjct: 8   HTFMTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKK 67

Query: 213 RRKLRGIPTDAGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKL 272
           RRK RGIP D GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKL
Sbjct: 68  RRKRRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKL 127

Query: 273 ISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCA 332
           ISKEFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCA
Sbjct: 128 ISKEFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCA 187

Query: 333 EWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTI 392
           EWF+AFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLM ISSSLTT+
Sbjct: 188 EWFVAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTV 247

Query: 393 ENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGS 452
            NIIYFEDAGYETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGS
Sbjct: 248 VNIIYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGS 307

Query: 453 TGLPKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPL 512
           TG PK                    GVMISH NIVAT AGV TVIPKL+ DDVYLAYLPL
Sbjct: 308 TGFPK--------------------GVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPL 367

Query: 513 AHVLELAAETVILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRV 572
           AHVLELAAE VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRV
Sbjct: 368 AHVLELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRV 427

Query: 573 RDGVLKKVEEKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGR 632
           RDGVLKKVEEKGGLVKKLFHI YNRQLA LEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR
Sbjct: 428 RDGVLKKVEEKGGLVKKLFHIAYNRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGR 487

Query: 633 IRFLLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCC 692
           +RF+LCGGAPLSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCC
Sbjct: 488 LRFMLCGGAPLSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCC 547

Query: 693 FIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGD 752
           FIKLVSWEEGGYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+EVYKDDEKG RWFYTGD
Sbjct: 548 FIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGD 607

Query: 753 IGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAV 812
           IGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAV
Sbjct: 608 IGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAV 667

Query: 813 VVPLRPALEQWAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLA 872
           VVPLRPALE+WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA
Sbjct: 668 VVPLRPALEEWAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILA 727

Query: 873 ADPWTPESGLVTAALKIKREQVKAKFKNELQQLYK 908
            DPWTPESGLVTAALKIKREQVKAKFK++LQQLYK
Sbjct: 728 PDPWTPESGLVTAALKIKREQVKAKFKDQLQQLYK 742

BLAST of Clc02G03070 vs. NCBI nr
Match: XP_038887007.1 (long chain acyl-CoA synthetase 8 [Benincasa hispida])

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 680/752 (90.43%), Postives = 704/752 (93.62%), Query Frame = 0

Query: 156 MTGDSIESIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRK 215
           MTGDS+E+  K KI+EN H G YLSVWRGYG VGIVGTIFIGILIAILLPSV LV+KR K
Sbjct: 1   MTGDSMETTWKPKILENLHAGVYLSVWRGYGTVGIVGTIFIGILIAILLPSVILVKKRWK 60

Query: 216 LRGIPTDAGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 275
            RGIPTD GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK
Sbjct: 61  RRGIPTDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 120

Query: 276 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWF 335
           EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGH+VD+RVAIYAETCAEWF
Sbjct: 121 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHNVDSRVAIYAETCAEWF 180

Query: 336 IAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENI 395
           IAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLR LMTISSSLTTIENI
Sbjct: 181 IAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMTISSSLTTIENI 240

Query: 396 IYFEDAGYETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 455
           IYFEDAGYETDFISSEN +KWKV SFSEVIKLG ENPVVPSLPSS SIAVVMYTSGSTGL
Sbjct: 241 IYFEDAGYETDFISSENTSKWKVMSFSEVIKLGKENPVVPSLPSSQSIAVVMYTSGSTGL 300

Query: 456 PKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHV 515
           PK                    GVMI H NIVATAAGVMTVIPKLD DDVYLAYLPLAHV
Sbjct: 301 PK--------------------GVMIGHGNIVATAAGVMTVIPKLDSDDVYLAYLPLAHV 360

Query: 516 LELAAETVILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDG 575
           LELAAETVILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDR+RDG
Sbjct: 361 LELAAETVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRIRDG 420

Query: 576 VLKKVEEKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRF 635
           VLKKVEE+GGLVKKLFHIGYNRQLAALEGSWLGAWGLE+LFWNVL+FRNIRSVLGGRIRF
Sbjct: 421 VLKKVEEEGGLVKKLFHIGYNRQLAALEGSWLGAWGLERLFWNVLVFRNIRSVLGGRIRF 480

Query: 636 LLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIK 695
           +LCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIK
Sbjct: 481 MLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIK 540

Query: 696 LVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGA 755
           LVSWEEGGYRTTDKPMPRGEIV+GGTSVT GYFKNPEKT+EV+KDD KGMRWFYTGDIG 
Sbjct: 541 LVSWEEGGYRTTDKPMPRGEIVIGGTSVTAGYFKNPEKTNEVFKDDGKGMRWFYTGDIGT 600

Query: 756 FHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVP 815
           FHPDGCLEIIDRKKDIVKLQHGEYISLGK EAALLSSKYVDNVMV+ADP+ +YCVAVVVP
Sbjct: 601 FHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALLSSKYVDNVMVYADPYRDYCVAVVVP 660

Query: 816 LRPALEQWAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADP 875
           LRPALE+WAKEAGIN+GDFSQLCEN ETISEVQ SLSKVAKDAKLQKFEIPE+IMLA DP
Sbjct: 661 LRPALEKWAKEAGINYGDFSQLCENGETISEVQRSLSKVAKDAKLQKFEIPEKIMLAPDP 720

Query: 876 WTPESGLVTAALKIKREQVKAKFKNELQQLYK 908
           WTP+SGLVTAALKIKREQVKAKFKNELQQLYK
Sbjct: 721 WTPDSGLVTAALKIKREQVKAKFKNELQQLYK 732

BLAST of Clc02G03070 vs. ExPASy Swiss-Prot
Match: Q9SJD4 (Long chain acyl-CoA synthetase 8 OS=Arabidopsis thaliana OX=3702 GN=LACS8 PE=1 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 1.6e-291
Identity = 487/733 (66.44%), Postives = 584/733 (79.67%), Query Frame = 0

Query: 174 HPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTDAGGDPGYAVRN 233
           +P D  S    +G+ GI+G   + +L+ +LL  V    K+ K RG+P   GG+ GY +R+
Sbjct: 7   NPMDSPSKGSDFGVYGIIGGGIVALLVPVLLSVVLNGTKKGKKRGVPIKVGGEEGYTMRH 66

Query: 234 ARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGE 293
           AR  EL  VPW+GA TM ALFEQSC+K+S+++ LGTR+ I KEF+TASDGRKFEKL+LGE
Sbjct: 67  ARAPELVDVPWEGAATMPALFEQSCKKYSKDRLLGTREFIDKEFITASDGRKFEKLHLGE 126

Query: 294 YEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCIRQDITVVTIYA 353
           Y+WQ+YGEVF+R CNFASGLV  GH+VD RVAI+++T AEWFIAFQGC RQ ITVVTIYA
Sbjct: 127 YKWQSYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIYA 186

Query: 354 SLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAGYETDFISSENM 413
           SLGEEALI+SLNET VSTLIC+SKQL+ L  I SSL T++NIIY E+ G +       +M
Sbjct: 187 SLGEEALIYSLNETRVSTLICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVNSM 246

Query: 414 NKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRGGDREDFIEKKC 473
               V+S SEV KLG +N V P LPS + +AV+M+TSGSTGLPK                
Sbjct: 247 GDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPK---------------- 306

Query: 474 GMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAETVILSAGLAMGY 533
               GVMI+H N+VATAAGVM V+PKLDK+D Y+AYLPLAHV EL AE V+ ++G A+GY
Sbjct: 307 ----GVMITHGNLVATAAGVMKVVPKLDKNDTYIAYLPLAHVFELEAEIVVFTSGSAIGY 366

Query: 534 GSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHI 593
           GS +T+TDTS+K+K+GTKGD S LKPTIMTAVPAILDRVR+GVLKKVEEKGG+ K LF  
Sbjct: 367 GSAMTLTDTSNKVKKGTKGDVSALKPTIMTAVPAILDRVREGVLKKVEEKGGMAKTLFDF 426

Query: 594 GYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAPLSGESQRFFNI 653
            Y R+LAA++GSW GAWGLEK+ W+ L+F+ IR+VLGG IRF+L GGAPLS +SQRF NI
Sbjct: 427 AYKRRLAAVDGSWFGAWGLEKMLWDALVFKKIRAVLGGHIRFMLVGGAPLSPDSQRFINI 486

Query: 654 CMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDKPMPR 713
           CMG+ IGQGYGLTET AGA FSEWDD +VGRVGPPLPC ++KLVSWEEGGYR +DKPMPR
Sbjct: 487 CMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKLVSWEEGGYRISDKPMPR 546

Query: 714 GEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCLEIIDRKKDIVK 773
           GEIVVGG SVT GYF N EKTDEVYK DEKG RWFYTGDIG FHPDGCLE+IDRKKDIVK
Sbjct: 547 GEIVVGGNSVTAGYFNNQEKTDEVYKVDEKGTRWFYTGDIGRFHPDGCLEVIDRKKDIVK 606

Query: 774 LQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALEQWAKEAGINFGD 833
           LQHGEY+SLGK EAAL SS YVDN+MVHADP ++YCVA+VVP R ALE+WA+EAG+   +
Sbjct: 607 LQHGEYVSLGKVEAALGSSNYVDNIMVHADPINSYCVALVVPSRGALEKWAEEAGVKHSE 666

Query: 834 FSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESGLVTAALKIKREQ 893
           F++LCE  E + EVQ SL+K  K AKL+KFE+P +I L ++PWTPESGLVTAALKIKREQ
Sbjct: 667 FAELCEKGEAVKEVQQSLTKAGKAAKLEKFELPAKIKLLSEPWTPESGLVTAALKIKREQ 719

Query: 894 VKAKFKNELQQLY 907
           +K+KFK+EL +LY
Sbjct: 727 IKSKFKDELSKLY 719

BLAST of Clc02G03070 vs. ExPASy Swiss-Prot
Match: Q9CAP8 (Long chain acyl-CoA synthetase 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LACS9 PE=1 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 2.3e-261
Identity = 436/710 (61.41%), Postives = 543/710 (76.48%), Query Frame = 0

Query: 197 GILIAILLPSVFLVRKRRKLRGIPTDAGGDPGYAVRNARVSELSQVPWKGATTMAALFEQ 256
           G+++ + L  +    K+ K RG+  D GG+PGYA+RN R +E     W+  +T+  LFE 
Sbjct: 7   GVIVPLALTFLVQKSKKEKKRGVVVDVGGEPGYAIRNHRFTEPVSSHWEHISTLPELFEI 66

Query: 257 SCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGF 316
           SC  HS   FLGTRKLIS+E  T+ DG+ FEKL+LG+YEW T+G+  +  C+FASGLV  
Sbjct: 67  SCNAHSDRVFLGTRKLISREIETSEDGKTFEKLHLGDYEWLTFGKTLEAVCDFASGLVQI 126

Query: 317 GHDVDTRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNS 376
           GH  + RVAI+A+T  EWFI+ QGC R+++TVVTIY+SLGEEAL HSLNETEV+T+IC S
Sbjct: 127 GHKTEERVAIFADTREEWFISLQGCFRRNVTVVTIYSSLGEEALCHSLNETEVTTVICGS 186

Query: 377 KQLRNLMTISSSLTTIENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKLGMENPVVPS 436
           K+L+ LM IS  L T++ +I  +D     +F S  N N W  TSF++V KLG ENPV P+
Sbjct: 187 KELKKLMDISQQLETVKRVICMDD-----EFPSDVNSN-WMATSFTDVQKLGRENPVDPN 246

Query: 437 LPSSHSIAVVMYTSGSTGLPKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGVMTV 496
            P S  +AV+MYTSGSTGLPK                    GVM++H N++AT + VMT+
Sbjct: 247 FPLSADVAVIMYTSGSTGLPK--------------------GVMMTHGNVLATVSAVMTI 306

Query: 497 IPKLDKDDVYLAYLPLAHVLELAAETVILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASV 556
           +P L K D+Y+AYLPLAH+LELAAE+V+ + G A+GYGSPLT+TDTS+KIK+GTKGD + 
Sbjct: 307 VPDLGKRDIYMAYLPLAHILELAAESVMATIGSAIGYGSPLTLTDTSNKIKKGTKGDVTA 366

Query: 557 LKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLF 616
           LKPTIMTAVPAILDRVRDGV KKV+ KGGL KKLF   Y R+L+A+ GSW GAWGLEKL 
Sbjct: 367 LKPTIMTAVPAILDRVRDGVRKKVDAKGGLSKKLFDFAYARRLSAINGSWFGAWGLEKLL 426

Query: 617 WNVLIFRNIRSVLGGRIRFLLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSE 676
           W+VL+FR IR+VLGG+IR+LL GGAPLSG++QRF NIC+GA IGQGYGLTET AG  FSE
Sbjct: 427 WDVLVFRKIRAVLGGQIRYLLSGGAPLSGDTQRFINICVGAPIGQGYGLTETCAGGTFSE 486

Query: 677 WDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDE 736
           ++DTSVGRVG PLPC F+KLV W EGGY T+DKPMPRGEIV+GG+++TLGYFKN EKT E
Sbjct: 487 FEDTSVGRVGAPLPCSFVKLVDWAEGGYLTSDKPMPRGEIVIGGSNITLGYFKNEEKTKE 546

Query: 737 VYKDDEKGMRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVD 796
           VYK DEKGMRWFYTGDIG FHPDGCLEIIDRKKDIVKLQHGEY+SLGK EAAL  S YV+
Sbjct: 547 VYKVDEKGMRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSISPYVE 606

Query: 797 NVMVHADPFHNYCVAVVVPLRPALEQWAKEAGINFGDFSQLCENRETISEVQGSLSKVAK 856
           N+MVHAD F++YCVA+VV  +  +E WA + GI+F +F +LC   + + EV  SL K AK
Sbjct: 607 NIMVHADSFYSYCVALVVASQHTVEGWASKQGIDFANFEELCTKEQAVKEVYASLVKAAK 666

Query: 857 DAKLQKFEIPERIMLAADPWTPESGLVTAALKIKREQVKAKFKNELQQLY 907
            ++L+KFEIP +I L A PWTPESGLVTAALK+KR+ ++ +F  +L +LY
Sbjct: 667 QSRLEKFEIPAKIKLLASPWTPESGLVTAALKLKRDVIRREFSEDLTKLY 690

BLAST of Clc02G03070 vs. ExPASy Swiss-Prot
Match: O95573 (Long-chain-fatty-acid--CoA ligase 3 OS=Homo sapiens OX=9606 GN=ACSL3 PE=1 SV=3)

HSP 1 Score: 577.4 bits (1487), Expect = 2.8e-163
Identity = 318/701 (45.36%), Postives = 439/701 (62.62%), Query Frame = 0

Query: 212 KRRKLRGIPTDAGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRK 271
           K  +++  P ++  D  Y   N+ +  L+ V + G  T+  +F  +  K    + LGTR+
Sbjct: 51  KSNRIKAKPVNSKPDSAYRSVNS-LDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTRE 110

Query: 272 LISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETC 331
           ++++E     +G+ F+K+ LG+Y W +Y +VF RA NF +GL   G    T +AI+ ET 
Sbjct: 111 VLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPKTNIAIFCETR 170

Query: 332 AEWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLR-NLMTISSSLT 391
           AEW IA Q C   +  +VT+YA+LG  A++H+LNETEV+ +I + + L+  L  I S + 
Sbjct: 171 AEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVP 230

Query: 392 TIENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKLG----MEN-PVVPSLPSSHSIAV 451
            + +II  +  G    +  SE      V + + V  LG    MEN P    LPS   IAV
Sbjct: 231 RLRHIITVD--GKPPTW--SEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPS--DIAV 290

Query: 452 VMYTSGSTGLPKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDV 511
           +MYTSGSTGLPK                    GVMISHSNI+A   G+   IP+L ++DV
Sbjct: 291 IMYTSGSTGLPK--------------------GVMISHSNIIAGITGMAERIPELGEEDV 350

Query: 512 YLAYLPLAHVLELAAETVILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAV 571
           Y+ YLPLAHVLEL+AE V LS G  +GY SP T+ D SSKIK+G+KGD S+LKPT+M AV
Sbjct: 351 YIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAV 410

Query: 572 PAILDRVRDGVLKKVEEKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNI 631
           P I+DR+   V+ KV E     + LF + YN ++  +      + G      +  +FR +
Sbjct: 411 PEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQI------SKGRNTPLCDSFVFRKV 470

Query: 632 RSVLGGRIRFLLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRV 691
           RS+LGG IR LLCGGAPLS  +QRF NIC    +GQGYGLTE+      SE  D + GRV
Sbjct: 471 RSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRV 530

Query: 692 GPPLPCCFIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGM 751
           G PL CC IKL +WEEGGY  TDKP PRGEI++GG SVT+GY+KN  KT   + +DE G 
Sbjct: 531 GAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDENGQ 590

Query: 752 RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPF 811
           RW  TGDIG F PDGCL+IIDRKKD+VKLQ GEY+SLGK EAAL +   VDN+  +A+ +
Sbjct: 591 RWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSY 650

Query: 812 HNYCVAVVVPLRPALEQWAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEI 871
           H+Y +  VVP +  L + A++ G+  G + +LC + E  +EV   LS+ A  A L+KFEI
Sbjct: 651 HSYVIGFVVPNQKELTELARKKGLK-GTWEELCNSCEMENEVLKVLSEAAISASLEKFEI 710

Query: 872 PERIMLAADPWTPESGLVTAALKIKREQVKAKFKNELQQLY 907
           P +I L+ +PWTPE+GLVT A K+KR+++K  ++ +++++Y
Sbjct: 711 PVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMY 717

BLAST of Clc02G03070 vs. ExPASy Swiss-Prot
Match: Q5R668 (Long-chain-fatty-acid--CoA ligase 3 OS=Pongo abelii OX=9601 GN=ACSL3 PE=2 SV=2)

HSP 1 Score: 577.0 bits (1486), Expect = 3.7e-163
Identity = 318/701 (45.36%), Postives = 438/701 (62.48%), Query Frame = 0

Query: 212 KRRKLRGIPTDAGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRK 271
           K  +++  P ++  D  Y   N+ +  L+ V + G  T+  +F  +  K    + LGTR+
Sbjct: 51  KSNRIKAKPVNSKPDSAYRSVNS-LDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTRE 110

Query: 272 LISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETC 331
           ++++E     +G+ F+K+ LG+Y W +Y +VF RA NF +GL   G    T +AI+ ET 
Sbjct: 111 VLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPKTNIAIFCETR 170

Query: 332 AEWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLR-NLMTISSSLT 391
           AEW IA Q C   +  +VT+YA+LG  A++H+LNETEV+ +I + + L+  L  I S + 
Sbjct: 171 AEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVP 230

Query: 392 TIENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKLG----MEN-PVVPSLPSSHSIAV 451
            + +II  +  G    +  SE      V + + V  LG    MEN P    LPS   IAV
Sbjct: 231 RLRHIITVD--GKPPTW--SEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPS--DIAV 290

Query: 452 VMYTSGSTGLPKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDV 511
           +MYTSGSTGLPK                    GVMISHSNI+A   G+   IP+L ++DV
Sbjct: 291 IMYTSGSTGLPK--------------------GVMISHSNIIAGITGMAERIPELGEEDV 350

Query: 512 YLAYLPLAHVLELAAETVILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAV 571
           Y+ YLPLAHVLEL+AE V LS G  +GY SP T+ D SSKIK+G+KGD S+LKPT+M AV
Sbjct: 351 YIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAV 410

Query: 572 PAILDRVRDGVLKKVEEKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNI 631
           P I+DR+   V+ KV E     + LF + YN ++  +      + G      N  +FR +
Sbjct: 411 PEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQI------SKGRNTPLCNSFVFRKV 470

Query: 632 RSVLGGRIRFLLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRV 691
           RS+LGG IR LLCGGAPLS  +QRF NIC    +GQGYGLTE+      SE  D + GRV
Sbjct: 471 RSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRV 530

Query: 692 GPPLPCCFIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGM 751
           G PL CC IKL +WEEGGY  TDKP PRGEI++GG  VT+GY+KN  KT   + +DE G 
Sbjct: 531 GAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQIVTMGYYKNEAKTKADFFEDENGQ 590

Query: 752 RWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPF 811
           RW  TGDIG F PDGCL+IIDRKKD+VKLQ GEY+SLGK EAAL +   VDN+  +A+ +
Sbjct: 591 RWLCTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSY 650

Query: 812 HNYCVAVVVPLRPALEQWAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEI 871
           H+Y +  VVP +  L + A++ G+  G + +LC + E  +EV   LS+ A  A L+KFEI
Sbjct: 651 HSYVIGFVVPNQKELTELARKKGLK-GTWEELCNSCEMENEVLKVLSEAAISASLEKFEI 710

Query: 872 PERIMLAADPWTPESGLVTAALKIKREQVKAKFKNELQQLY 907
           P +I L+ +PWTPE+GLVT A K+KR+++K  ++ +++++Y
Sbjct: 711 PVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMY 717

BLAST of Clc02G03070 vs. ExPASy Swiss-Prot
Match: Q63151 (Long-chain-fatty-acid--CoA ligase 3 OS=Rattus norvegicus OX=10116 GN=Acsl3 PE=1 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 9.1e-162
Identity = 318/722 (44.04%), Postives = 443/722 (61.36%), Query Frame = 0

Query: 195 FIGILIAIL--LPSVFLVRKRRK----LRGIPTDAGGDPGYAVRNARVSELSQVPWKGAT 254
           FI  L  IL  +P  FL   +++    ++  P  +  D  Y   N+ +  L+ V + G  
Sbjct: 28  FIISLYTILTYIPFYFLCESKQEKPNHIKAKPVSSKPDSAYRSVNS-MDGLASVLYPGCD 87

Query: 255 TMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACN 314
           T+  +F  +  K    + LGTR+++++E     +G+ F+K+ LG Y W +Y +VF RA +
Sbjct: 88  TLDKVFMYAKNKFKDKRLLGTREILNEEDEIQPNGKVFKKVILGHYNWLSYEDVFIRALD 147

Query: 315 FASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETE 374
           F +GL   G      +AI+ ET AEW IA Q C   +  +VT+YA+LG  A++H LNETE
Sbjct: 148 FGNGLQMLGQKPKANIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHGLNETE 207

Query: 375 VSTLICNSKQLR-NLMTISSSLTTIENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKL 434
           V+ +I + + L+  L  I S +  + +II  +  G    +  SE      V + + V  L
Sbjct: 208 VTNIITSKELLQTKLKDIVSLVPRLRHIITVD--GKPPTW--SEFPKGVIVHTMAAVQAL 267

Query: 435 GMENPV---VPSLPSSHSIAVVMYTSGSTGLPKKNQRGGDREDFIEKKCGMDWGVMISHS 494
           G++  V     S P    IAV+MYTSGSTG+PK                    GVMISHS
Sbjct: 268 GVKADVDKKAHSKPLPSDIAVIMYTSGSTGIPK--------------------GVMISHS 327

Query: 495 NIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAETVILSAGLAMGYGSPLTMTDTSS 554
           NI+A+  G+   IP+L ++DVY+ YLPLAHVLEL+AE V LS G  +GY SP T+ D SS
Sbjct: 328 NIIASITGMARRIPRLGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSS 387

Query: 555 KIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIGYNRQLAALEG 614
           KIK+G+KGD SVLKPT+M AVP I+DR+   V+ KV E     + LF + YN ++  +  
Sbjct: 388 KIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMNKVNEMSAFQRNLFILAYNYKMEQI-- 447

Query: 615 SWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAPLSGESQRFFNICMGATIGQGYG 674
               + G      +  +FRN+R +LGG IR LLCGGAPLS  +QRF NIC    +GQGYG
Sbjct: 448 ----SKGCSTPLCDRFVFRNVRRLLGGNIRVLLCGGAPLSATTQRFMNICFCCPVGQGYG 507

Query: 675 LTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVT 734
           LTE+      +E  D + GRVG PL CC IKL +WEEGGY  TDKP PRGEI++GG +VT
Sbjct: 508 LTESTGAGTITEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQNVT 567

Query: 735 LGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGK 794
           +GY+KN  KT   + +DE G RW  TGDIG F PDGCL+IIDRKKD+VKLQ GEY+SLGK
Sbjct: 568 MGYYKNEAKTKADFFEDENGQRWLCTGDIGEFDPDGCLKIIDRKKDLVKLQAGEYVSLGK 627

Query: 795 AEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALEQWAKEAGINFGDFSQLCENRETI 854
            EAAL +   +DN+  +A+ +H+Y +  VVP +  L + A+  G N G + +LC + E  
Sbjct: 628 VEAALKNLPLIDNICAYANSYHSYVIGFVVPNQKELTELARTKGFN-GTWEELCNSSEME 687

Query: 855 SEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESGLVTAALKIKREQVKAKFKNELQQ 907
           +EV   LS+ A  A L+KFEIP +I L+ DPWTPE+GLVT A K+KR+++K  ++ ++++
Sbjct: 688 NEVLKVLSEAAISASLEKFEIPLKIRLSPDPWTPETGLVTDAFKLKRKELKTHYQADIER 717

BLAST of Clc02G03070 vs. ExPASy TrEMBL
Match: A0A1S4E3L6 (long chain acyl-CoA synthetase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500501 PE=4 SV=1)

HSP 1 Score: 1378.6 bits (3567), Expect = 0.0e+00
Identity = 691/805 (85.84%), Postives = 721/805 (89.57%), Query Frame = 0

Query: 103 VSYCYRSTAPASPLVYVCFELVDHLLEKQSCDVSVIMKFEDAQFVIDSIAYTFMTGDSIE 162
           VSYCYRSTAP SPL  +CF+                              +TFMTG+SIE
Sbjct: 2   VSYCYRSTAPPSPLFDLCFK-----------------------------GHTFMTGESIE 61

Query: 163 SIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTD 222
           SIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+KRRK RGIP D
Sbjct: 62  SIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVD 121

Query: 223 AGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASD 282
            GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISKEFVT SD
Sbjct: 122 VGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISKEFVTTSD 181

Query: 283 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCI 342
           GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCAEWF+AFQGCI
Sbjct: 182 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWFVAFQGCI 241

Query: 343 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAG 402
           RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLM ISSSLTT+ NIIYFEDAG
Sbjct: 242 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNIIYFEDAG 301

Query: 403 YETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRG 462
           YETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG PK     
Sbjct: 302 YETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGFPK----- 361

Query: 463 GDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAET 522
                          GVMISH NIVAT AGV TVIPKL+ DDVYLAYLPLAHVLELAAE 
Sbjct: 362 ---------------GVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHVLELAAEA 421

Query: 523 VILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKKVEE 582
           VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRVRDGVLKKVEE
Sbjct: 422 VILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEE 481

Query: 583 KGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAP 642
           KGGLVKKLFHI YNRQLA LEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR+RF+LCGGAP
Sbjct: 482 KGGLVKKLFHIAYNRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAP 541

Query: 643 LSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG 702
           LSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG
Sbjct: 542 LSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG 601

Query: 703 GYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCL 762
           GYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+EVYKDDEKG RWFYTGDIGAFHPDGCL
Sbjct: 602 GYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGCL 661

Query: 763 EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALEQ 822
           EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALE+
Sbjct: 662 EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEE 721

Query: 823 WAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESGL 882
           WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA DPWTPESGL
Sbjct: 722 WAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGL 757

Query: 883 VTAALKIKREQVKAKFKNELQQLYK 908
           VTAALKIKREQVKAKFK++LQQLYK
Sbjct: 782 VTAALKIKREQVKAKFKDQLQQLYK 757

BLAST of Clc02G03070 vs. ExPASy TrEMBL
Match: A0A5A7UTI1 (Long chain acyl-CoA synthetase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G001400 PE=4 SV=1)

HSP 1 Score: 1375.5 bits (3559), Expect = 0.0e+00
Identity = 692/806 (85.86%), Postives = 722/806 (89.58%), Query Frame = 0

Query: 103 VSYCYRSTAPASPLVYVCFELVDHLLEKQSCDVSVIMKFEDAQFVIDSIAYTFMTGDSIE 162
           VSYCYRSTAP SPL  +CF+                              +TFMTG+SIE
Sbjct: 2   VSYCYRSTAPPSPLFDLCFK-----------------------------GHTFMTGESIE 61

Query: 163 SIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTD 222
           SIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+KRRK RGIP D
Sbjct: 62  SIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVD 121

Query: 223 AGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASD 282
            GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISKEFVT SD
Sbjct: 122 VGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISKEFVTTSD 181

Query: 283 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCI 342
           GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCAEWF+AFQGCI
Sbjct: 182 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWFVAFQGCI 241

Query: 343 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAG 402
           RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLR LM ISSSLTT+ NIIYFEDAG
Sbjct: 242 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMEISSSLTTVVNIIYFEDAG 301

Query: 403 YETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRG 462
           YETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG PK     
Sbjct: 302 YETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGFPK----- 361

Query: 463 GDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAET 522
                          GVMISH NIVAT AGVMTVIPKL+ DDVYLAYLPLAHVLELAAE 
Sbjct: 362 ---------------GVMISHGNIVATVAGVMTVIPKLNSDDVYLAYLPLAHVLELAAEA 421

Query: 523 VILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKK-VE 582
           VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRVRDGVLKK VE
Sbjct: 422 VILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVVE 481

Query: 583 EKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGA 642
           EKGGLVKKLFHI YNRQLAALEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR+RF+LCGGA
Sbjct: 482 EKGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGA 541

Query: 643 PLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEE 702
           PLSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEE
Sbjct: 542 PLSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEE 601

Query: 703 GGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGC 762
           GGYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+EVYKDDEKG RWFYTGDIGAFHPDGC
Sbjct: 602 GGYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGAFHPDGC 661

Query: 763 LEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALE 822
           LEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALE
Sbjct: 662 LEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALE 721

Query: 823 QWAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESG 882
           +WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA DPWTPESG
Sbjct: 722 EWAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESG 758

Query: 883 LVTAALKIKREQVKAKFKNELQQLYK 908
           LVTAALKIKREQVKAKFK++LQQLYK
Sbjct: 782 LVTAALKIKREQVKAKFKDQLQQLYK 758

BLAST of Clc02G03070 vs. ExPASy TrEMBL
Match: A0A5D3D6X5 (Long chain acyl-CoA synthetase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00280 PE=4 SV=1)

HSP 1 Score: 1368.6 bits (3541), Expect = 0.0e+00
Identity = 689/805 (85.59%), Postives = 719/805 (89.32%), Query Frame = 0

Query: 103 VSYCYRSTAPASPLVYVCFELVDHLLEKQSCDVSVIMKFEDAQFVIDSIAYTFMTGDSIE 162
           VSYCYRSTAP SPL  +CF+                              +TFMTG+SIE
Sbjct: 2   VSYCYRSTAPPSPLFDLCFK-----------------------------GHTFMTGESIE 61

Query: 163 SIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTD 222
           SIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+KRRK RGIP D
Sbjct: 62  SIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRKRRGIPVD 121

Query: 223 AGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASD 282
            GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISKEFVT SD
Sbjct: 122 VGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISKEFVTTSD 181

Query: 283 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCI 342
           GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCAEWF+AFQGCI
Sbjct: 182 GRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWFVAFQGCI 241

Query: 343 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAG 402
           RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLR LM ISSSLTT+ NIIYFEDAG
Sbjct: 242 RQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRKLMEISSSLTTVVNIIYFEDAG 301

Query: 403 YETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRG 462
           YETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG PK     
Sbjct: 302 YETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGFPK----- 361

Query: 463 GDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAET 522
                          GVMISH NIVAT AGVMTVIPKL+ DDVYLAYLPLAHVLELAAE 
Sbjct: 362 ---------------GVMISHGNIVATVAGVMTVIPKLNSDDVYLAYLPLAHVLELAAEA 421

Query: 523 VILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKKVEE 582
           VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRVRDGVLKKVEE
Sbjct: 422 VILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDGVLKKVEE 481

Query: 583 KGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAP 642
           KGGLVKKLFHI YNRQLAALEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR+RF+LCGGAP
Sbjct: 482 KGGLVKKLFHIAYNRQLAALEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRFMLCGGAP 541

Query: 643 LSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG 702
           LSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG
Sbjct: 542 LSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEG 601

Query: 703 GYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCL 762
           GYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+E   DDEKG RWFYTGDIGAFHPDGCL
Sbjct: 602 GYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNE---DDEKGTRWFYTGDIGAFHPDGCL 661

Query: 763 EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALEQ 822
           EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVPLRPALE+
Sbjct: 662 EIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVPLRPALEE 721

Query: 823 WAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESGL 882
           WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA DPWTPESGL
Sbjct: 722 WAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDPWTPESGL 754

Query: 883 VTAALKIKREQVKAKFKNELQQLYK 908
           VTAALKIKREQVKAKFK++LQQLYK
Sbjct: 782 VTAALKIKREQVKAKFKDQLQQLYK 754

BLAST of Clc02G03070 vs. ExPASy TrEMBL
Match: A0A1S4E3L7 (long chain acyl-CoA synthetase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500501 PE=4 SV=1)

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 676/755 (89.54%), Postives = 704/755 (93.25%), Query Frame = 0

Query: 153 YTFMTGDSIESIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRK 212
           +TFMTG+SIESIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+K
Sbjct: 8   HTFMTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKK 67

Query: 213 RRKLRGIPTDAGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKL 272
           RRK RGIP D GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKL
Sbjct: 68  RRKRRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKL 127

Query: 273 ISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCA 332
           ISKEFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCA
Sbjct: 128 ISKEFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCA 187

Query: 333 EWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTI 392
           EWF+AFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLM ISSSLTT+
Sbjct: 188 EWFVAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTV 247

Query: 393 ENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGS 452
            NIIYFEDAGYETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGS
Sbjct: 248 VNIIYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGS 307

Query: 453 TGLPKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPL 512
           TG PK                    GVMISH NIVAT AGV TVIPKL+ DDVYLAYLPL
Sbjct: 308 TGFPK--------------------GVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPL 367

Query: 513 AHVLELAAETVILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRV 572
           AHVLELAAE VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRV
Sbjct: 368 AHVLELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRV 427

Query: 573 RDGVLKKVEEKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGR 632
           RDGVLKKVEEKGGLVKKLFHI YNRQLA LEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR
Sbjct: 428 RDGVLKKVEEKGGLVKKLFHIAYNRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGR 487

Query: 633 IRFLLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCC 692
           +RF+LCGGAPLSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCC
Sbjct: 488 LRFMLCGGAPLSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCC 547

Query: 693 FIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGD 752
           FIKLVSWEEGGYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+EVYKDDEKG RWFYTGD
Sbjct: 548 FIKLVSWEEGGYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGD 607

Query: 753 IGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAV 812
           IGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAV
Sbjct: 608 IGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAV 667

Query: 813 VVPLRPALEQWAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLA 872
           VVPLRPALE+WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA
Sbjct: 668 VVPLRPALEEWAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILA 727

Query: 873 ADPWTPESGLVTAALKIKREQVKAKFKNELQQLYK 908
            DPWTPESGLVTAALKIKREQVKAKFK++LQQLYK
Sbjct: 728 PDPWTPESGLVTAALKIKREQVKAKFKDQLQQLYK 742

BLAST of Clc02G03070 vs. ExPASy TrEMBL
Match: A0A1S3CGI4 (long chain acyl-CoA synthetase 8 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103500501 PE=4 SV=1)

HSP 1 Score: 1358.2 bits (3514), Expect = 0.0e+00
Identity = 674/752 (89.63%), Postives = 701/752 (93.22%), Query Frame = 0

Query: 156 MTGDSIESIRKSKIVENFHPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRK 215
           MTG+SIESIR SKI+EN H GDY  VWRGYGIVGIVGTIFIGILIAILLPSVFLV+KRRK
Sbjct: 1   MTGESIESIRNSKIIENLHAGDYSLVWRGYGIVGIVGTIFIGILIAILLPSVFLVKKRRK 60

Query: 216 LRGIPTDAGGDPGYAVRNARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISK 275
            RGIP D GGDPGYAVRNARV+ELSQVPWKGATTMAALFEQSCRKH+QNKFLGTRKLISK
Sbjct: 61  RRGIPVDVGGDPGYAVRNARVTELSQVPWKGATTMAALFEQSCRKHAQNKFLGTRKLISK 120

Query: 276 EFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWF 335
           EFVT SDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVG GHDVD+RVAIYAETCAEWF
Sbjct: 121 EFVTTSDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGIGHDVDSRVAIYAETCAEWF 180

Query: 336 IAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENI 395
           +AFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLM ISSSLTT+ NI
Sbjct: 181 VAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNSKQLRNLMEISSSLTTVVNI 240

Query: 396 IYFEDAGYETDFISSENMNKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGL 455
           IYFEDAGYETDFISSEN +KWKV SFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTG 
Sbjct: 241 IYFEDAGYETDFISSENTSKWKVISFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGF 300

Query: 456 PKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHV 515
           PK                    GVMISH NIVAT AGV TVIPKL+ DDVYLAYLPLAHV
Sbjct: 301 PK--------------------GVMISHGNIVATVAGVTTVIPKLNSDDVYLAYLPLAHV 360

Query: 516 LELAAETVILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDG 575
           LELAAE VILSAGLAMGYGSPLTMTDTSSKIK+GTKGD SVLKPTIMTAVPAILDRVRDG
Sbjct: 361 LELAAEAVILSAGLAMGYGSPLTMTDTSSKIKKGTKGDVSVLKPTIMTAVPAILDRVRDG 420

Query: 576 VLKKVEEKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRF 635
           VLKKVEEKGGLVKKLFHI YNRQLA LEGSWLGAWGLE+LFWNVLIFRNIRSVLGGR+RF
Sbjct: 421 VLKKVEEKGGLVKKLFHIAYNRQLAGLEGSWLGAWGLERLFWNVLIFRNIRSVLGGRLRF 480

Query: 636 LLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIK 695
           +LCGGAPLSGES RFFNICMG+TIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIK
Sbjct: 481 MLCGGAPLSGESLRFFNICMGSTIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIK 540

Query: 696 LVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGA 755
           LVSWEEGGYRTTD PMPRGEIV+GGTSV +GYFKNPEKT+EVYKDDEKG RWFYTGDIGA
Sbjct: 541 LVSWEEGGYRTTDIPMPRGEIVIGGTSVAVGYFKNPEKTNEVYKDDEKGTRWFYTGDIGA 600

Query: 756 FHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVP 815
           FHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMV+ADPFHNYCVAVVVP
Sbjct: 601 FHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVDNVMVYADPFHNYCVAVVVP 660

Query: 816 LRPALEQWAKEAGINFGDFSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADP 875
           LRPALE+WAKEAGIN+G+FSQLCEN E+ISEVQ SLSKV KDAKLQKFEIPE+I+LA DP
Sbjct: 661 LRPALEEWAKEAGINYGEFSQLCENGESISEVQRSLSKVGKDAKLQKFEIPEKIILAPDP 720

Query: 876 WTPESGLVTAALKIKREQVKAKFKNELQQLYK 908
           WTPESGLVTAALKIKREQVKAKFK++LQQLYK
Sbjct: 721 WTPESGLVTAALKIKREQVKAKFKDQLQQLYK 732

BLAST of Clc02G03070 vs. TAIR 10
Match: AT2G04350.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1003.4 bits (2593), Expect = 1.1e-292
Identity = 487/733 (66.44%), Postives = 584/733 (79.67%), Query Frame = 0

Query: 174 HPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTDAGGDPGYAVRN 233
           +P D  S    +G+ GI+G   + +L+ +LL  V    K+ K RG+P   GG+ GY +R+
Sbjct: 7   NPMDSPSKGSDFGVYGIIGGGIVALLVPVLLSVVLNGTKKGKKRGVPIKVGGEEGYTMRH 66

Query: 234 ARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGE 293
           AR  EL  VPW+GA TM ALFEQSC+K+S+++ LGTR+ I KEF+TASDGRKFEKL+LGE
Sbjct: 67  ARAPELVDVPWEGAATMPALFEQSCKKYSKDRLLGTREFIDKEFITASDGRKFEKLHLGE 126

Query: 294 YEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCIRQDITVVTIYA 353
           Y+WQ+YGEVF+R CNFASGLV  GH+VD RVAI+++T AEWFIAFQGC RQ ITVVTIYA
Sbjct: 127 YKWQSYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIYA 186

Query: 354 SLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAGYETDFISSENM 413
           SLGEEALI+SLNET VSTLIC+SKQL+ L  I SSL T++NIIY E+ G +       +M
Sbjct: 187 SLGEEALIYSLNETRVSTLICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVNSM 246

Query: 414 NKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRGGDREDFIEKKC 473
               V+S SEV KLG +N V P LPS + +AV+M+TSGSTGLPK                
Sbjct: 247 GDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPK---------------- 306

Query: 474 GMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAETVILSAGLAMGY 533
               GVMI+H N+VATAAGVM V+PKLDK+D Y+AYLPLAHV EL AE V+ ++G A+GY
Sbjct: 307 ----GVMITHGNLVATAAGVMKVVPKLDKNDTYIAYLPLAHVFELEAEIVVFTSGSAIGY 366

Query: 534 GSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHI 593
           GS +T+TDTS+K+K+GTKGD S LKPTIMTAVPAILDRVR+GVLKKVEEKGG+ K LF  
Sbjct: 367 GSAMTLTDTSNKVKKGTKGDVSALKPTIMTAVPAILDRVREGVLKKVEEKGGMAKTLFDF 426

Query: 594 GYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAPLSGESQRFFNI 653
            Y R+LAA++GSW GAWGLEK+ W+ L+F+ IR+VLGG IRF+L GGAPLS +SQRF NI
Sbjct: 427 AYKRRLAAVDGSWFGAWGLEKMLWDALVFKKIRAVLGGHIRFMLVGGAPLSPDSQRFINI 486

Query: 654 CMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDKPMPR 713
           CMG+ IGQGYGLTET AGA FSEWDD +VGRVGPPLPC ++KLVSWEEGGYR +DKPMPR
Sbjct: 487 CMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKLVSWEEGGYRISDKPMPR 546

Query: 714 GEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCLEIIDRKKDIVK 773
           GEIVVGG SVT GYF N EKTDEVYK DEKG RWFYTGDIG FHPDGCLE+IDRKKDIVK
Sbjct: 547 GEIVVGGNSVTAGYFNNQEKTDEVYKVDEKGTRWFYTGDIGRFHPDGCLEVIDRKKDIVK 606

Query: 774 LQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALEQWAKEAGINFGD 833
           LQHGEY+SLGK EAAL SS YVDN+MVHADP ++YCVA+VVP R ALE+WA+EAG+   +
Sbjct: 607 LQHGEYVSLGKVEAALGSSNYVDNIMVHADPINSYCVALVVPSRGALEKWAEEAGVKHSE 666

Query: 834 FSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESGLVTAALKIKREQ 893
           F++LCE  E + EVQ SL+K  K AKL+KFE+P +I L ++PWTPESGLVTAALKIKREQ
Sbjct: 667 FAELCEKGEAVKEVQQSLTKAGKAAKLEKFELPAKIKLLSEPWTPESGLVTAALKIKREQ 719

Query: 894 VKAKFKNELQQLY 907
           +K+KFK+EL +LY
Sbjct: 727 IKSKFKDELSKLY 719

BLAST of Clc02G03070 vs. TAIR 10
Match: AT2G04350.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1003.4 bits (2593), Expect = 1.1e-292
Identity = 487/733 (66.44%), Postives = 584/733 (79.67%), Query Frame = 0

Query: 174 HPGDYLSVWRGYGIVGIVGTIFIGILIAILLPSVFLVRKRRKLRGIPTDAGGDPGYAVRN 233
           +P D  S    +G+ GI+G   + +L+ +LL  V    K+ K RG+P   GG+ GY +R+
Sbjct: 7   NPMDSPSKGSDFGVYGIIGGGIVALLVPVLLSVVLNGTKKGKKRGVPIKVGGEEGYTMRH 66

Query: 234 ARVSELSQVPWKGATTMAALFEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGE 293
           AR  EL  VPW+GA TM ALFEQSC+K+S+++ LGTR+ I KEF+TASDGRKFEKL+LGE
Sbjct: 67  ARAPELVDVPWEGAATMPALFEQSCKKYSKDRLLGTREFIDKEFITASDGRKFEKLHLGE 126

Query: 294 YEWQTYGEVFDRACNFASGLVGFGHDVDTRVAIYAETCAEWFIAFQGCIRQDITVVTIYA 353
           Y+WQ+YGEVF+R CNFASGLV  GH+VD RVAI+++T AEWFIAFQGC RQ ITVVTIYA
Sbjct: 127 YKWQSYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIYA 186

Query: 354 SLGEEALIHSLNETEVSTLICNSKQLRNLMTISSSLTTIENIIYFEDAGYETDFISSENM 413
           SLGEEALI+SLNET VSTLIC+SKQL+ L  I SSL T++NIIY E+ G +       +M
Sbjct: 187 SLGEEALIYSLNETRVSTLICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVNSM 246

Query: 414 NKWKVTSFSEVIKLGMENPVVPSLPSSHSIAVVMYTSGSTGLPKKNQRGGDREDFIEKKC 473
               V+S SEV KLG +N V P LPS + +AV+M+TSGSTGLPK                
Sbjct: 247 GDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPK---------------- 306

Query: 474 GMDWGVMISHSNIVATAAGVMTVIPKLDKDDVYLAYLPLAHVLELAAETVILSAGLAMGY 533
               GVMI+H N+VATAAGVM V+PKLDK+D Y+AYLPLAHV EL AE V+ ++G A+GY
Sbjct: 307 ----GVMITHGNLVATAAGVMKVVPKLDKNDTYIAYLPLAHVFELEAEIVVFTSGSAIGY 366

Query: 534 GSPLTMTDTSSKIKQGTKGDASVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHI 593
           GS +T+TDTS+K+K+GTKGD S LKPTIMTAVPAILDRVR+GVLKKVEEKGG+ K LF  
Sbjct: 367 GSAMTLTDTSNKVKKGTKGDVSALKPTIMTAVPAILDRVREGVLKKVEEKGGMAKTLFDF 426

Query: 594 GYNRQLAALEGSWLGAWGLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAPLSGESQRFFNI 653
            Y R+LAA++GSW GAWGLEK+ W+ L+F+ IR+VLGG IRF+L GGAPLS +SQRF NI
Sbjct: 427 AYKRRLAAVDGSWFGAWGLEKMLWDALVFKKIRAVLGGHIRFMLVGGAPLSPDSQRFINI 486

Query: 654 CMGATIGQGYGLTETFAGAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDKPMPR 713
           CMG+ IGQGYGLTET AGA FSEWDD +VGRVGPPLPC ++KLVSWEEGGYR +DKPMPR
Sbjct: 487 CMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKLVSWEEGGYRISDKPMPR 546

Query: 714 GEIVVGGTSVTLGYFKNPEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCLEIIDRKKDIVK 773
           GEIVVGG SVT GYF N EKTDEVYK DEKG RWFYTGDIG FHPDGCLE+IDRKKDIVK
Sbjct: 547 GEIVVGGNSVTAGYFNNQEKTDEVYKVDEKGTRWFYTGDIGRFHPDGCLEVIDRKKDIVK 606

Query: 774 LQHGEYISLGKAEAALLSSKYVDNVMVHADPFHNYCVAVVVPLRPALEQWAKEAGINFGD 833
           LQHGEY+SLGK EAAL SS YVDN+MVHADP ++YCVA+VVP R ALE+WA+EAG+   +
Sbjct: 607 LQHGEYVSLGKVEAALGSSNYVDNIMVHADPINSYCVALVVPSRGALEKWAEEAGVKHSE 666

Query: 834 FSQLCENRETISEVQGSLSKVAKDAKLQKFEIPERIMLAADPWTPESGLVTAALKIKREQ 893
           F++LCE  E + EVQ SL+K  K AKL+KFE+P +I L ++PWTPESGLVTAALKIKREQ
Sbjct: 667 FAELCEKGEAVKEVQQSLTKAGKAAKLEKFELPAKIKLLSEPWTPESGLVTAALKIKREQ 719

Query: 894 VKAKFKNELQQLY 907
           +K+KFK+EL +LY
Sbjct: 727 IKSKFKDELSKLY 719

BLAST of Clc02G03070 vs. TAIR 10
Match: AT1G77590.1 (long chain acyl-CoA synthetase 9 )

HSP 1 Score: 903.3 bits (2333), Expect = 1.6e-262
Identity = 436/710 (61.41%), Postives = 543/710 (76.48%), Query Frame = 0

Query: 197 GILIAILLPSVFLVRKRRKLRGIPTDAGGDPGYAVRNARVSELSQVPWKGATTMAALFEQ 256
           G+++ + L  +    K+ K RG+  D GG+PGYA+RN R +E     W+  +T+  LFE 
Sbjct: 7   GVIVPLALTFLVQKSKKEKKRGVVVDVGGEPGYAIRNHRFTEPVSSHWEHISTLPELFEI 66

Query: 257 SCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGLVGF 316
           SC  HS   FLGTRKLIS+E  T+ DG+ FEKL+LG+YEW T+G+  +  C+FASGLV  
Sbjct: 67  SCNAHSDRVFLGTRKLISREIETSEDGKTFEKLHLGDYEWLTFGKTLEAVCDFASGLVQI 126

Query: 317 GHDVDTRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLICNS 376
           GH  + RVAI+A+T  EWFI+ QGC R+++TVVTIY+SLGEEAL HSLNETEV+T+IC S
Sbjct: 127 GHKTEERVAIFADTREEWFISLQGCFRRNVTVVTIYSSLGEEALCHSLNETEVTTVICGS 186

Query: 377 KQLRNLMTISSSLTTIENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKLGMENPVVPS 436
           K+L+ LM IS  L T++ +I  +D     +F S  N N W  TSF++V KLG ENPV P+
Sbjct: 187 KELKKLMDISQQLETVKRVICMDD-----EFPSDVNSN-WMATSFTDVQKLGRENPVDPN 246

Query: 437 LPSSHSIAVVMYTSGSTGLPKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGVMTV 496
            P S  +AV+MYTSGSTGLPK                    GVM++H N++AT + VMT+
Sbjct: 247 FPLSADVAVIMYTSGSTGLPK--------------------GVMMTHGNVLATVSAVMTI 306

Query: 497 IPKLDKDDVYLAYLPLAHVLELAAETVILSAGLAMGYGSPLTMTDTSSKIKQGTKGDASV 556
           +P L K D+Y+AYLPLAH+LELAAE+V+ + G A+GYGSPLT+TDTS+KIK+GTKGD + 
Sbjct: 307 VPDLGKRDIYMAYLPLAHILELAAESVMATIGSAIGYGSPLTLTDTSNKIKKGTKGDVTA 366

Query: 557 LKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIGYNRQLAALEGSWLGAWGLEKLF 616
           LKPTIMTAVPAILDRVRDGV KKV+ KGGL KKLF   Y R+L+A+ GSW GAWGLEKL 
Sbjct: 367 LKPTIMTAVPAILDRVRDGVRKKVDAKGGLSKKLFDFAYARRLSAINGSWFGAWGLEKLL 426

Query: 617 WNVLIFRNIRSVLGGRIRFLLCGGAPLSGESQRFFNICMGATIGQGYGLTETFAGAAFSE 676
           W+VL+FR IR+VLGG+IR+LL GGAPLSG++QRF NIC+GA IGQGYGLTET AG  FSE
Sbjct: 427 WDVLVFRKIRAVLGGQIRYLLSGGAPLSGDTQRFINICVGAPIGQGYGLTETCAGGTFSE 486

Query: 677 WDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKNPEKTDE 736
           ++DTSVGRVG PLPC F+KLV W EGGY T+DKPMPRGEIV+GG+++TLGYFKN EKT E
Sbjct: 487 FEDTSVGRVGAPLPCSFVKLVDWAEGGYLTSDKPMPRGEIVIGGSNITLGYFKNEEKTKE 546

Query: 737 VYKDDEKGMRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALLSSKYVD 796
           VYK DEKGMRWFYTGDIG FHPDGCLEIIDRKKDIVKLQHGEY+SLGK EAAL  S YV+
Sbjct: 547 VYKVDEKGMRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSISPYVE 606

Query: 797 NVMVHADPFHNYCVAVVVPLRPALEQWAKEAGINFGDFSQLCENRETISEVQGSLSKVAK 856
           N+MVHAD F++YCVA+VV  +  +E WA + GI+F +F +LC   + + EV  SL K AK
Sbjct: 607 NIMVHADSFYSYCVALVVASQHTVEGWASKQGIDFANFEELCTKEQAVKEVYASLVKAAK 666

Query: 857 DAKLQKFEIPERIMLAADPWTPESGLVTAALKIKREQVKAKFKNELQQLY 907
            ++L+KFEIP +I L A PWTPESGLVTAALK+KR+ ++ +F  +L +LY
Sbjct: 667 QSRLEKFEIPAKIKLLASPWTPESGLVTAALKLKRDVIRREFSEDLTKLY 690

BLAST of Clc02G03070 vs. TAIR 10
Match: AT3G05970.1 (long-chain acyl-CoA synthetase 6 )

HSP 1 Score: 360.5 bits (924), Expect = 3.9e-99
Identity = 217/657 (33.03%), Postives = 329/657 (50.08%), Query Frame = 0

Query: 254 FEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGL 313
           FE +       K+LGTR  +        DG       +G+Y+W TYGE         SGL
Sbjct: 90  FEHAVHDFRDYKYLGTRVRV--------DG------TVGDYKWMTYGEAGTARTALGSGL 149

Query: 314 VGFGHDVDTRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLI 373
           V  G  + + V IY     EW I    C       V +Y +LG +A+   +N   V  + 
Sbjct: 150 VHHGIPMGSSVGIYFINRPEWLIVDHACSSYSYVSVPLYDTLGPDAVKFIVNHATVQAIF 209

Query: 374 CNSKQLRNLMTISSSLTTIENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKLGMENPV 433
           C ++ L +L++  S + ++  ++     G      S  + +  KV S+S ++  G  NP 
Sbjct: 210 CVAETLNSLLSCLSEMPSVRLVVVV--GGLIESLPSLPSSSGVKVVSYSVLLNQGRSNPQ 269

Query: 434 VPSLPSSHSIAVVMYTSGSTGLPKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGV 493
               P    +A + YTSG+TG PK                    GV+++H+N++A  AG 
Sbjct: 270 RFFPPKPDDVATICYTSGTTGTPK--------------------GVVLTHANLIANVAGS 329

Query: 494 MTVIPKLDKDDVYLAYLPLAHVLELAAETVILSAGLAMGY--GSPLTMTDTSSKIKQGTK 553
              + K    DVY++YLPLAH+ E A + + +  G+A+G+  G  + + D          
Sbjct: 330 SFSV-KFFSSDVYISYLPLAHIYERANQILTVYFGVAVGFYQGDNMKLLD---------- 389

Query: 554 GDASVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIGYN-RQLAALEGSWLGAW 613
            D + L+PT+ ++VP + +R+  G++  V+  GGL ++LF+  YN ++ A L G      
Sbjct: 390 -DLAALRPTVFSSVPRLYNRIYAGIINAVKTSGGLKERLFNAAYNAKKQALLNGKSASP- 449

Query: 614 GLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAPLSGESQRFFNICMGATIGQGYGLTETFA 673
                 W+ L+F  I+  LGGR+RF+  G +PLS E   F  +C G  + +GYG+TET  
Sbjct: 450 -----IWDRLVFNKIKDRLGGRVRFMTSGASPLSPEVMEFLKVCFGGRVTEGYGMTETSC 509

Query: 674 GAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKN 733
             +  +  D   G VG P P C +KLV   E  Y + D+P PRGEI V G  +  GY+K+
Sbjct: 510 VISGMDEGDNLTGHVGSPNPACEVKLVDVPEMNYTSADQPHPRGEICVRGPIIFTGYYKD 569

Query: 734 PEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALL 793
             +T EV  +D     W +TGDIG + P G L+IIDRKK+I KL  GEYI+  K E    
Sbjct: 570 EIQTKEVIDED----GWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVYA 629

Query: 794 SSKYVDNVMVHADPFHNYCVAVVVPLRPALEQWAKEAGINFGDFSQLCENRETISEVQGS 853
             K+V    ++ D F++  VAVV      L+ WA   GI  GD  +LC N    + V   
Sbjct: 630 KCKFVGQCFIYGDSFNSSLVAVVSVDPDVLKSWAASEGIKGGDLRELCNNPRVKAAVLSD 688

Query: 854 LSKVAKDAKLQKFEIPERIMLAADPWTPESGLVTAALKIKREQVKAKFKNELQQLYK 908
           +  V ++A+L+ FE  + + L  +P+T E+GL+T   KIKR Q K  F   +  +YK
Sbjct: 690 MDTVGREAQLRGFEFAKAVTLVLEPFTLENGLLTPTFKIKRPQAKEYFAEAITNMYK 688

BLAST of Clc02G03070 vs. TAIR 10
Match: AT5G27600.1 (long-chain acyl-CoA synthetase 7 )

HSP 1 Score: 351.7 bits (901), Expect = 1.8e-96
Identity = 212/656 (32.32%), Postives = 326/656 (49.70%), Query Frame = 0

Query: 254 FEQSCRKHSQNKFLGTRKLISKEFVTASDGRKFEKLNLGEYEWQTYGEVFDRACNFASGL 313
           F  +   +++NK+LGTR          SDG       +GEY W TYGE         SGL
Sbjct: 90  FVHAVETYAENKYLGTR--------VRSDG------TIGEYSWMTYGEAASERQAIGSGL 149

Query: 314 VGFGHDVDTRVAIYAETCAEWFIAFQGCIRQDITVVTIYASLGEEALIHSLNETEVSTLI 373
           +  G +    V +Y     EW +    C       V +Y +LG +A+   +N   +  + 
Sbjct: 150 LFHGVNQGDCVGLYFINRPEWLVVDHACAAYSFVSVPLYDTLGPDAVKFVVNHANLQAIF 209

Query: 374 CNSKQLRNLMTISSSLTTIENIIYFEDAGYETDFISSENMNKWKVTSFSEVIKLGMENPV 433
           C  + L  L++  + + +I  I+     G +    S        + S+ +++  G  +  
Sbjct: 210 CVPQTLNILLSFLAEIPSIRLIVVV--GGADEHLPSLPRGTGVTIVSYQKLLSQGRSSLH 269

Query: 434 VPSLPSSHSIAVVMYTSGSTGLPKKNQRGGDREDFIEKKCGMDWGVMISHSNIVATAAGV 493
             S P    IA + YTSG+TG PK                    GV+++H N++A  AG 
Sbjct: 270 PFSPPKPEDIATICYTSGTTGTPK--------------------GVVLTHGNLIANVAG- 329

Query: 494 MTVIPKLDKDDVYLAYLPLAHVLELAAETVILSAGLAMGY--GSPLTMTDTSSKIKQGTK 553
            +V  +    DVY++YLPLAH+ E A + + +  G+A+G+  G    + D          
Sbjct: 330 SSVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMD---------- 389

Query: 554 GDASVLKPTIMTAVPAILDRVRDGVLKKVEEKGGLVKKLFHIGYN-RQLAALEGSWLGAW 613
            D +VL+PTI  +VP + +R+ DG+   V+  G + K+LF I YN ++ A + G    A 
Sbjct: 390 -DFAVLRPTIFCSVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSA- 449

Query: 614 GLEKLFWNVLIFRNIRSVLGGRIRFLLCGGAPLSGESQRFFNICMGATIGQGYGLTETFA 673
                FW+ L+F  I+  LGGR+RF+  G +PLS +   F  IC G ++ +GYG+TET  
Sbjct: 450 -----FWDKLVFNKIKEKLGGRVRFMGSGASPLSPDVMDFLRICFGCSVREGYGMTETSC 509

Query: 674 GAAFSEWDDTSVGRVGPPLPCCFIKLVSWEEGGYRTTDKPMPRGEIVVGGTSVTLGYFKN 733
             +  +  D   G VG P P C +KLV   E  Y + D+P PRGEI V G  +  GY+K+
Sbjct: 510 VISAMDDGDNLSGHVGSPNPACEVKLVDVPEMNYTSDDQPYPRGEICVRGPIIFKGYYKD 569

Query: 734 PEKTDEVYKDDEKGMRWFYTGDIGAFHPDGCLEIIDRKKDIVKLQHGEYISLGKAEAALL 793
            E+T E+   D     W +TGDIG + P G L+IIDRKK+I KL  GEYI+  K E    
Sbjct: 570 EEQTREILDGD----GWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVYT 629

Query: 794 SSKYVDNVMVHADPFHNYCVAVVVPLRPALEQWAKEAGINFGDFSQLCENRETISEVQGS 853
             ++V    +H D F++  VA+V      ++ WA   GI +    QLC +      V   
Sbjct: 630 KCRFVSQCFIHGDSFNSSLVAIVSVDPEVMKDWAASEGIKYEHLGQLCNDPRVRKTVLAE 687

Query: 854 LSKVAKDAKLQKFEIPERIMLAADPWTPESGLVTAALKIKREQVKAKFKNELQQLY 907
           +  + ++A+L+ FE  + + L  +P+T E+GL+T   KIKR Q KA F   + ++Y
Sbjct: 690 MDDLGREAQLRGFEFAKAVTLVPEPFTLENGLLTPTFKIKRPQAKAYFAEAISKMY 687