Clc02G01180 (gene) Watermelon (cordophanus) v2

BLAST of Clc02G01180 vs. NCBI nr
Match: XP_038902853.1 (probable pectinesterase/pectinesterase inhibitor 20 [Benincasa hispida])

HSP 1 Score: 986.5 bits (2549), Expect = 9.4e-284
Identity = 491/555 (88.47%), Postives = 522/555 (94.05%), Query Frame = 0

Query: 1   MASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFT 60
           M SSN ISLLTFLTFLFLL SS SAVVPT EIC+ST  PSFCN+FLPNS ASVHSHCRFT
Sbjct: 1   MVSSNSISLLTFLTFLFLLPSSFSAVVPTTEICRSTTFPSFCNAFLPNSPASVHSHCRFT 60

Query: 61  LRNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQ 120
           L +AL HA  FLALVD+HLSLFP  SALHDCR LAEANLD+I+QTFSIVNST++ LPYYQ
Sbjct: 61  LHHALTHAQTFLALVDAHLSLFPFPSALHDCRSLAEANLDYITQTFSIVNSTSVALPYYQ 120

Query: 121 AHEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVSE 180
           AHEM+SLMSAMITNE+TCY+GL GLGSA+GLVDKVLEAISFDRKLYSLYLSLFKMGWVS+
Sbjct: 121 AHEMMSLMSAMITNENTCYEGLAGLGSAIGLVDKVLEAISFDRKLYSLYLSLFKMGWVSK 180

Query: 181 DMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILIN 240
           +MKAP+LP MNHFGVGKGQLKLKMSPKDRAYYERLVH KKPAAARRLLQTNY++DGIL+N
Sbjct: 181 NMKAPILPKMNHFGVGKGQLKLKMSPKDRAYYERLVHSKKPAAARRLLQTNYENDGILVN 240

Query: 241 GMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMMG 300
           G+VAVDQNG YDFT IS AIAAAP+K+ V  GYFLIFVTAGIYNE+VLVPK KRYVL++G
Sbjct: 241 GVVAVDQNGTYDFTNISAAIAAAPDKTTVTKGYFLIFVTAGIYNETVLVPKNKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSADN 360
           EGNNQTII GNKNV+DGSTTFNSATVAVEG GFFGVNLTISNTAG AKHQAVALRVSADN
Sbjct: 301 EGNNQTIINGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGPAKHQAVALRVSADN 360

Query: 361 VTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSFI 480
           ALTAQGRTDPNQNTGTSIHNCTIK T ELAA+PATKSYLGRPWKEYSRT+YMQSFID+FI
Sbjct: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAANPATKSYLGRPWKEYSRTVYMQSFIDAFI 480

Query: 481 DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGDQ 540
           DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRA WVVGVINATVA++FTVSQFLAGDQ
Sbjct: 481 DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRAPWVVGVINATVANSFTVSQFLAGDQ 540

Query: 541 WLPPTAVPYTGGLIS 556
           WLPPTAVPYTGGLIS
Sbjct: 541 WLPPTAVPYTGGLIS 555

BLAST of Clc02G01180 vs. NCBI nr
Match: XP_008457895.1 (PREDICTED: pectinesterase-like [Cucumis melo] >KAA0045890.1 pectinesterase-like [Cucumis melo var. makuwa] >TYK13697.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 956.8 bits (2472), Expect = 8.0e-275
Identity = 477/556 (85.79%), Postives = 512/556 (92.09%), Query Frame = 0

Query: 1   MASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFT 60
           M SSNPIS+LTFLT LF LS SSSA VPTAEICQST SP FCN FLPNS ASVHSHCRFT
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-VPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFT 60

Query: 61  LRNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNS-TAMVLPYY 120
           L +AL HA  FL+LV++HL+LFPSLS LHDCR LAEANLDF+SQTFSIVNS T  +LPYY
Sbjct: 61  LHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYY 120

Query: 121 QAHEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 180
            AHE ++LMSA+ITNEDTCY+GL GL SA+GLVDKVLEAISFD+KLYSLYLSLFKMGWVS
Sbjct: 121 DAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVS 180

Query: 181 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILI 240
           +D+KAP LP M HFGVGKGQLKLKMSPKDRAYYERLVHR KP  ARRLLQTNYQDDGI+I
Sbjct: 181 KDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVI 240

Query: 241 NGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMM 300
           NG+V VDQNG YDFT I+ AIAAAPNK+ VA GYFLIFV AGIYNE+VL+PK KRYVL++
Sbjct: 241 NGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLI 300

Query: 301 GEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSAD 360
           GEGNNQTIITGNKNV+DGSTTFNSATVAVEG GF GVNLTISNTAGAAKHQAVALRVSAD
Sbjct: 301 GEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSAD 360

Query: 361 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQF 420
           N TLY+CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP+SGQF
Sbjct: 361 NATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 420

Query: 421 NALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSF 480
           NA+TAQGRTDPNQNTGTSIHNCTIK T ELAASPATKSYLGRPWK+YSRT+YMQSFIDSF
Sbjct: 421 NAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSF 480

Query: 481 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGD 540
           IDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS+R SW VGVINATVA++FTVSQFLAGD
Sbjct: 481 IDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGD 540

Query: 541 QWLPPTAVPYTGGLIS 556
           +WLPPT VPYTGGLIS
Sbjct: 541 RWLPPTDVPYTGGLIS 555

BLAST of Clc02G01180 vs. NCBI nr
Match: XP_004148470.1 (pectinesterase [Cucumis sativus] >KAE8651957.1 hypothetical protein Csa_006047 [Cucumis sativus])

HSP 1 Score: 948.7 bits (2451), Expect = 2.2e-272
Identity = 472/555 (85.05%), Postives = 504/555 (90.81%), Query Frame = 0

Query: 1   MASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFT 60
           M SSNPISLLTFLT LFLLS SSSA  PTA+ICQST SP FCNSFLP S  S+HSHCRFT
Sbjct: 1   MVSSNPISLLTFLTLLFLLSPSSSA-APTADICQSTTSPPFCNSFLPKSPDSIHSHCRFT 60

Query: 61  LRNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQ 120
           L  AL HA  FL+LV++ L+L PSLSALHDCR LAEANLDF+ QTFSIVNST   LPYY 
Sbjct: 61  LHQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYD 120

Query: 121 AHEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVSE 180
           AHEM+SL+SA+ITN DTCY+GL  L SA+GLVDKVLEAISFD+KLYSLYLSLFKMGWVS+
Sbjct: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180

Query: 181 DMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILIN 240
           D+KAP  P MNHFG GKGQLKLKMSPKDRAYYERLVHR KP  ARRLLQTNYQDDGI++N
Sbjct: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240

Query: 241 GMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMMG 300
           G+V VDQNG YDFT I+ AIAAAPNK+ VA GYFLIFV AGIYNE+VLVPK KRYVL++G
Sbjct: 241 GIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSADN 360
           EGNNQTIITGNKNV+DGSTTFNSATVAVEG GF GVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360

Query: 361 VTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
           VTLY+CIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLPMSGQFN
Sbjct: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSFI 480
           ALTAQGRTDPNQNTGTSIHNCTIK T ELAASPATKSYLGRPWK+YSRT+YMQSFIDSFI
Sbjct: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480

Query: 481 DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGDQ 540
           DP GWKEWDGTLNLNTSYYAEFNNSGPG DTS+RASW VGVINATVASNFTVSQ LAGD+
Sbjct: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540

Query: 541 WLPPTAVPYTGGLIS 556
           WLPPT VPYTGGLIS
Sbjct: 541 WLPPTEVPYTGGLIS 554

BLAST of Clc02G01180 vs. NCBI nr
Match: KAG6571391.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 899.0 bits (2322), Expect = 2.0e-257
Identity = 453/556 (81.47%), Postives = 494/556 (88.85%), Query Frame = 0

Query: 2   ASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFTL 61
           +SSNPISLL F + LFL +SSS+  VPT E+CQS  SPSFC SFLP+S ASVHSHCR+TL
Sbjct: 3   SSSNPISLLLFFSLLFLFTSSSA--VPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTL 62

Query: 62  RNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQA 121
            +AL  A  F+ LVD  L+LFPSLSALHDCR LAEANLDFIS T   VN+T   LP+YQA
Sbjct: 63  HHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAEANLDFISSTVDTVNTTTNALPFYQA 122

Query: 122 HEMVSLMSAMITNEDTCYDGL--TGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 181
            EM+SL+SA+ITN DTC+DGL  +G GS LGLVDKV++AISFDR+LYSLYLSLFKMGWVS
Sbjct: 123 DEMISLLSAVITNVDTCHDGLAGSGSGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVS 182

Query: 182 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILI 241
           ++MKAP LP  NHFG G G LKLKMSPKDRAYYERLVH KKP AARRLLQTNY DDGIL+
Sbjct: 183 KNMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVHGKKP-AARRLLQTNYADDGILV 242

Query: 242 NGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMM 301
           NG+VAVDQNGAYDFTTI+DAIAAAPNKS  A+GYFLIFVTAGIY ESV+VPK K+YVLM+
Sbjct: 243 NGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMI 302

Query: 302 GEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSAD 361
           GEGNN+TIITGNKNV+DGSTTFNSATV VEG GFFG NLTI+NTAGA KHQAVALRVSAD
Sbjct: 303 GEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSAD 362

Query: 362 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQF 421
             T YSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP+SGQF
Sbjct: 363 MTTFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 422

Query: 422 NALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSF 481
           NALTAQGRTD NQNTG  IH CTIKPT +LAASPATKSYLGRPWKE+SRT+YMQSFID+F
Sbjct: 423 NALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFSRTVYMQSFIDAF 482

Query: 482 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGD 541
           IDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTSRRA W VGVINATVA+ FTVS+ LAGD
Sbjct: 483 IDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGD 542

Query: 542 QWLPPTAVPYTGGLIS 556
           QWLPPTAVPY+GGLIS
Sbjct: 543 QWLPPTAVPYSGGLIS 555

BLAST of Clc02G01180 vs. NCBI nr
Match: KAG7011156.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894.0 bits (2309), Expect = 6.4e-256
Identity = 451/556 (81.12%), Postives = 493/556 (88.67%), Query Frame = 0

Query: 2   ASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFTL 61
           +SSNPISLL F + LFL +SSS+  VPT E+CQS  SPSFC SFLP+S+ASVHSHCR+TL
Sbjct: 3   SSSNPISLLLFFSLLFLFTSSSA--VPTVEVCQSATSPSFCKSFLPSSSASVHSHCRYTL 62

Query: 62  RNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQA 121
            +AL  A  F+ LVD  L+LFPSLSALHDCR LA ANLDFIS T   VN+T   L +YQA
Sbjct: 63  HHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALQFYQA 122

Query: 122 HEMVSLMSAMITNEDTCYDGL--TGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 181
            EM+SL+SA+ITN DTC+DGL  +G GS LGLVDKV++AISFDR+LYSLYLSLFKMGWVS
Sbjct: 123 DEMISLLSAVITNVDTCHDGLAGSGSGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVS 182

Query: 182 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILI 241
           ++MKAP LP  NHFG G G LKLKMSPKDRAYYERLVH KKP AARRLLQTNY DDGIL+
Sbjct: 183 KNMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVHGKKP-AARRLLQTNYADDGILV 242

Query: 242 NGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMM 301
           NG+VAVDQNGAYDFTTI+DAIAAAPNKS  A+GYFLIFVTAGIY ESV+VPK K+YVLM+
Sbjct: 243 NGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMI 302

Query: 302 GEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSAD 361
           GEGNN+TIITGNKNV+DGSTTFNSATV VEG GFFG NLTI+NTAGA KHQAVALRVSAD
Sbjct: 303 GEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSAD 362

Query: 362 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQF 421
             T YSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP+SGQF
Sbjct: 363 MTTFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 422

Query: 422 NALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSF 481
           NALTAQGRTD NQNTG  IH CTIKPT +LAASPATKSYLGRPWKE+SRT+YMQSFID+F
Sbjct: 423 NALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFSRTVYMQSFIDAF 482

Query: 482 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGD 541
           IDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTSRRA W VGVINATVA+ FTVS+ LAGD
Sbjct: 483 IDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGD 542

Query: 542 QWLPPTAVPYTGGLIS 556
           QWLPPTAVPY+GGLIS
Sbjct: 543 QWLPPTAVPYSGGLIS 555

BLAST of Clc02G01180 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 520.4 bits (1339), Expect = 2.5e-146
Identity = 286/570 (50.18%), Postives = 368/570 (64.56%), Query Frame = 0

Query: 13  LTFLFLLSSSSSAVVP--------------TAEICQSTASPSFCNSFLPNSAASVHSHCR 72
           L FLF L+  SS   P               + IC+    PS+C S LPN    ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 73  FTLRNALDHAHAFLALVDSHL------SLFPSLSALHDCRGLAEANLDFISQTFSIVNST 132
            +LR +L  A  F++++D+ L      +   ++ AL DC+ LA   +D++  +    +ST
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST 124

Query: 133 AMVLPYYQAHEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSL 192
              L   +A ++ + +SA ITNE TC +GL    S  GL   +      D KLY + L+L
Sbjct: 125 -KTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFN----DTKLYGVSLAL 184

Query: 193 FKMGWV-----SEDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRL 252
           F  GWV     S  +  P       FG   G+L LKM+ + RA Y  +  RK       L
Sbjct: 185 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRK-------L 244

Query: 253 LQTNYQDDGILINGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESV 312
           LQ++   D + ++ +V V QNG  +FTTI+ AIAAAPNK+  ++GYFLI+VTAG+Y E V
Sbjct: 245 LQSD--ADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYV 304

Query: 313 LVPKYKRYVLMMGEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAA 372
            VPK KRYV+M+G+G NQT+ITGN++V+DG TTFNSAT  + G  F GVN+TI NTAG  
Sbjct: 305 EVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPT 364

Query: 373 KHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNC 432
           K QAVALR   D    YSC FE YQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNC
Sbjct: 365 KGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNC 424

Query: 433 NIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPAT-KSYLGRPWKEY 492
           N+Y R P  GQ N +TAQGRTDPNQNTGT+IH CTI+P  +LA S  T K+YLGRPWKEY
Sbjct: 425 NLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEY 484

Query: 493 SRTIYMQSFIDSFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINAT 552
           SRT+ MQ++ID F++P+GW  W G   L+T YYAE+NN+GPGSDT+ R +W    VINAT
Sbjct: 485 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINAT 544

Query: 553 VASNFTVSQFLAGDQWLPPTAVPYTGGLIS 556
            ASNFTV+ FL G+ W+  T VP+ GGLI+
Sbjct: 545 DASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of Clc02G01180 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 503.1 bits (1294), Expect = 4.2e-141
Identity = 283/579 (48.88%), Postives = 371/579 (64.08%), Query Frame = 0

Query: 7   ISLLTFLTFLFL----LSSSSSAVVP---TAEICQSTASPSFCNSFLP-NSAASVHSHCR 66
           +SL  FL  LFL    L  +S  ++P   ++ IC++T  P FC S  P  S   V  + R
Sbjct: 2   LSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGR 61

Query: 67  FTLRNALDHAHAFLALVDSHLSLFPSL------SALHDCRGLAEANLDFISQTFSIVNST 126
           F+LR +L  +  F   +D +L    +L       AL DCR LA    D++  +F  VN T
Sbjct: 62  FSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNIT 121

Query: 127 --AMVLPYYQAHEMVSLMSAMITNEDTCYDGL-TGLGSALGLVDKVLEAISFDRKLYSLY 186
             +  L + +A E+ +L+SA +TNE TC DG+ T   S+  + + V   +  D KL+S+ 
Sbjct: 122 TSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVS 181

Query: 187 LSLFKMGWVSEDMKAPML-----PNMNHFGV------GKGQLKLKMSPKDRAYYERLVHR 246
           L+LF  GWV +  K         P   H           G L LKM+   RA YE L  R
Sbjct: 182 LALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRR 241

Query: 247 KKPAAARRLLQTNYQDDGILINGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFV 306
           K       L   +   + +L++ +V V+QNG  +FTTI++A+ +APNK+    GYF+I+V
Sbjct: 242 K-------LADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYV 301

Query: 307 TAGIYNESVLVPKYKRYVLMMGEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNL 366
           T+G+Y E+V++ K KRY++M+G+G N+T++TGN+NV+DG TTFNSAT AV    F  VN+
Sbjct: 302 TSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNM 361

Query: 367 TISNTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFG 426
           T  NTAG  KHQAVA+R SAD    YSC FE YQDTLYTHSLRQFYRECDIYGTVDFIFG
Sbjct: 362 TFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFG 421

Query: 427 NAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPAT-KS 486
           NAAVV Q+CN+Y R PM  QFNA+TAQGRTDPNQNTG SIHNCTIKP  +L +S  T K+
Sbjct: 422 NAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKT 481

Query: 487 YLGRPWKEYSRTIYMQSFIDSFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASW 546
           YLGRPWKEYSRT++MQS+ID  ++P GW+EW+G   L+T YYAE+NN+G GS T+ R  W
Sbjct: 482 YLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVW 541

Query: 547 V-VGVINATVASNFTVSQFLAGDQWLPPTAVPYTGGLIS 556
               VIN+T A+NFTV  FL GD W+  + VPY  GL+S
Sbjct: 542 PGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of Clc02G01180 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.3e-139
Identity = 283/584 (48.46%), Postives = 368/584 (63.01%), Query Frame = 0

Query: 9   LLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFTLRNALDHA 68
           L+T   FL  + +SS  +  ++ IC++T  P +C S  P+S  +V  +  F++R +L  +
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 69  HAFLALVD------SHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTA-----MVLP 128
             F+  VD      +HLS    + AL DCR LA   +D++  +F  VN T+       L 
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 129 YYQAHEMVSLMSAMITNEDTCYDGLT-----------GLGSALGLVDKVLEAISFDRKLY 188
           + +A ++ +L+SA +TNE TC +GLT             G AL LV+        D KL 
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVN--------DTKLL 187

Query: 189 SLYLSLFKMGWVSEDMK-------APMLPNMNHFG----VGKGQLKLKMSPKDRAYYERL 248
            + L+LF  GWV +  K        P   +  H         G L LKM+ K +A YE L
Sbjct: 188 GVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESL 247

Query: 249 VHRKKPAAARRLLQTNYQDDG--ILINGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGY 308
             RK              DDG  +LI+ +V V Q+G  +FT I+ A+AAAPN +  + G+
Sbjct: 248 SRRKLADGD----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGF 307

Query: 309 FLIFVTAGIYNESVLVPKYKRYVLMMGEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGF 368
           FLI+VTAGIY E + + K KRY++M+G+G NQT++TGN++V+DG TTFNSAT AV    F
Sbjct: 308 FLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNF 367

Query: 369 FGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTV 428
             VN+T  NTAG  KHQAVALR  AD    YSC FE YQDTLYTHSLRQFYRECD+YGTV
Sbjct: 368 VAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTV 427

Query: 429 DFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTSELAASP 488
           DFIFGNAAVV QNCN+Y R PM  QFNA+TAQGR+DPNQNTGTSI NCTIKP  +L +S 
Sbjct: 428 DFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN 487

Query: 489 AT-KSYLGRPWKEYSRTIYMQSFIDSFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTS 548
            T K+YLGRPWKEYSRT+YMQS+ID F++P GW+EW+G   L+T YYAE+NN+GPGS+T+
Sbjct: 488 YTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTT 547

Query: 549 RRASWV-VGVINATVASNFTVSQFLAGDQWLPPTAVPYTGGLIS 556
            R +W    VIN+T A+NFTV+       W+  T VPYT GLIS
Sbjct: 548 NRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of Clc02G01180 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 4.3e-114
Identity = 234/547 (42.78%), Postives = 328/547 (59.96%), Query Frame = 0

Query: 33  CQSTASPSFCNSFL---PNSAASVHSHCRFTLRNALDHAHAFLALVDSHLSLFPS----- 92
           C+ST  P  C + L    +S +  + + +FT++  L  A     ++ S+     S     
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 93  ----LSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQAHEMVSLMSAMITNEDTCYD 152
               + A+ DC  L+E +++++ +T +    TA V+       + SL+S ++TN+ TC D
Sbjct: 143 TAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 153 GLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKAPMLPNMNHFGVGKGQL 212
           GL    S  G    +   +    +LYS+      +G VS  +      N+  F   KG++
Sbjct: 203 GLVEAKS--GFAAAIGSPMGNLTRLYSI-----SLGLVSHALNR----NLKRFKASKGKI 262

Query: 213 KLKMSPKDRAYYERLVHRKKPA---------AARRLLQTNYQDDG-ILINGMVAVDQNGA 272
               +   R   E L+   +            +R L +      G IL++  V V    +
Sbjct: 263 LGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 322

Query: 273 YDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMMGEGNNQTIITG 332
            +FTTI+DAIAAAPN +   DGYF+I+   G+Y E ++VP  K+ +++MG+G N+TIITG
Sbjct: 323 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 382

Query: 333 NKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEG 392
           N NV+DG TT+N ++ AV G  F  V++T  NTAG  KHQAVALR +A+  + Y C FEG
Sbjct: 383 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 442

Query: 393 YQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDP 452
           YQDTLY HSLRQFYRECDIYGTVDFIFGNAA + QNCNIYAR PM+ Q NA+TA GR DP
Sbjct: 443 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 502

Query: 453 NQNTGTSIHNCTIKPTSELAASP-ATKSYLGRPWKEYSRTIYMQSFIDSFIDPAGWKEWD 512
           NQNTG SI NCTIK   +LAA P +  ++LGRPWK YSRT++MQS+I   + P GW EW+
Sbjct: 503 NQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWN 562

Query: 513 GTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVASNFTVSQFLAGDQWLPPTAVP 556
           GT+ L+T YY E++N GPG++T++R  W+   ++N   A NFTV  F  GD WLP T +P
Sbjct: 563 GTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIP 617

BLAST of Clc02G01180 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 6.5e-110
Identity = 230/551 (41.74%), Postives = 321/551 (58.26%), Query Frame = 0

Query: 20  SSSSSAVVPTAEICQSTASPSFCNSFL---PNSAASVHSHCRFTLRNALDHAHAFLALV- 79
           S S  + +P    C+ST  P  C + L    +S +  + + +FT++  L  A     ++ 
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 80  --------DSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQAHEMVSL 139
                   D   S    +SA+ DC  LAE +++++ +T +     A ++       + SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSL 194

Query: 140 MSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKAPML 199
           +  ++TN+ TC DGL    S  G    +   +    +LYS+ L L     ++ ++K    
Sbjct: 195 LGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKG 254

Query: 200 PNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDG--ILINGMVAV 259
                FG G   ++  +    +   +     K    A R L    +  G  IL+   V V
Sbjct: 255 SKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314

Query: 260 DQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMMGEGNNQ 319
                 +F TI++A+AAAPN +    GYF+I+  AG+Y E V++   KR ++++G+G N+
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374

Query: 320 TIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYS 379
           TII+GN + IDG TT+NS+T AV G  F  V++T  NTAG  KHQAVA+R +AD  T Y 
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434

Query: 380 CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 439
           C FEGYQDTLY HSLRQFYRECDIYGT+DFIFGNAA + QNCNIYAR PM+ Q NA+TA 
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494

Query: 440 GRTDPNQNTGTSIHNCTIKPTSELAASP-ATKSYLGRPWKEYSRTIYMQSFIDSFIDPAG 499
           GRTDPNQ TG SI NCTI    +LAA P +T ++LGRPWK YSRT+Y+QS+I   + P G
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 554

Query: 500 WKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVASNFTVSQFLAGDQWLP 555
           W EW+GT  L+T  Y E++N GPG+DTS+R  W    ++N   A NFTV  F  GD WLP
Sbjct: 555 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 614

BLAST of Clc02G01180 vs. ExPASy TrEMBL
Match: A0A5A7TRW1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002210 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 3.9e-275
Identity = 477/556 (85.79%), Postives = 512/556 (92.09%), Query Frame = 0

Query: 1   MASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFT 60
           M SSNPIS+LTFLT LF LS SSSA VPTAEICQST SP FCN FLPNS ASVHSHCRFT
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-VPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFT 60

Query: 61  LRNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNS-TAMVLPYY 120
           L +AL HA  FL+LV++HL+LFPSLS LHDCR LAEANLDF+SQTFSIVNS T  +LPYY
Sbjct: 61  LHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYY 120

Query: 121 QAHEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 180
            AHE ++LMSA+ITNEDTCY+GL GL SA+GLVDKVLEAISFD+KLYSLYLSLFKMGWVS
Sbjct: 121 DAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVS 180

Query: 181 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILI 240
           +D+KAP LP M HFGVGKGQLKLKMSPKDRAYYERLVHR KP  ARRLLQTNYQDDGI+I
Sbjct: 181 KDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVI 240

Query: 241 NGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMM 300
           NG+V VDQNG YDFT I+ AIAAAPNK+ VA GYFLIFV AGIYNE+VL+PK KRYVL++
Sbjct: 241 NGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLI 300

Query: 301 GEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSAD 360
           GEGNNQTIITGNKNV+DGSTTFNSATVAVEG GF GVNLTISNTAGAAKHQAVALRVSAD
Sbjct: 301 GEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSAD 360

Query: 361 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQF 420
           N TLY+CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP+SGQF
Sbjct: 361 NATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 420

Query: 421 NALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSF 480
           NA+TAQGRTDPNQNTGTSIHNCTIK T ELAASPATKSYLGRPWK+YSRT+YMQSFIDSF
Sbjct: 421 NAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSF 480

Query: 481 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGD 540
           IDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS+R SW VGVINATVA++FTVSQFLAGD
Sbjct: 481 IDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGD 540

Query: 541 QWLPPTAVPYTGGLIS 556
           +WLPPT VPYTGGLIS
Sbjct: 541 RWLPPTDVPYTGGLIS 555

BLAST of Clc02G01180 vs. ExPASy TrEMBL
Match: A0A1S3C644 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497470 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 3.9e-275
Identity = 477/556 (85.79%), Postives = 512/556 (92.09%), Query Frame = 0

Query: 1   MASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFT 60
           M SSNPIS+LTFLT LF LS SSSA VPTAEICQST SP FCN FLPNS ASVHSHCRFT
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-VPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFT 60

Query: 61  LRNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNS-TAMVLPYY 120
           L +AL HA  FL+LV++HL+LFPSLS LHDCR LAEANLDF+SQTFSIVNS T  +LPYY
Sbjct: 61  LHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYY 120

Query: 121 QAHEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVS 180
            AHE ++LMSA+ITNEDTCY+GL GL SA+GLVDKVLEAISFD+KLYSLYLSLFKMGWVS
Sbjct: 121 DAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVS 180

Query: 181 EDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILI 240
           +D+KAP LP M HFGVGKGQLKLKMSPKDRAYYERLVHR KP  ARRLLQTNYQDDGI+I
Sbjct: 181 KDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVI 240

Query: 241 NGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMM 300
           NG+V VDQNG YDFT I+ AIAAAPNK+ VA GYFLIFV AGIYNE+VL+PK KRYVL++
Sbjct: 241 NGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLI 300

Query: 301 GEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSAD 360
           GEGNNQTIITGNKNV+DGSTTFNSATVAVEG GF GVNLTISNTAGAAKHQAVALRVSAD
Sbjct: 301 GEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSAD 360

Query: 361 NVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQF 420
           N TLY+CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP+SGQF
Sbjct: 361 NATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 420

Query: 421 NALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSF 480
           NA+TAQGRTDPNQNTGTSIHNCTIK T ELAASPATKSYLGRPWK+YSRT+YMQSFIDSF
Sbjct: 421 NAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSF 480

Query: 481 IDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGD 540
           IDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS+R SW VGVINATVA++FTVSQFLAGD
Sbjct: 481 IDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGD 540

Query: 541 QWLPPTAVPYTGGLIS 556
           +WLPPT VPYTGGLIS
Sbjct: 541 RWLPPTDVPYTGGLIS 555

BLAST of Clc02G01180 vs. ExPASy TrEMBL
Match: A0A6J1EJM8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 4.0e-256
Identity = 448/554 (80.87%), Postives = 489/554 (88.27%), Query Frame = 0

Query: 2   ASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFTL 61
           +SSNPISLL F + LFL +SSS+  VPT E+CQS  SPSFC SFLP+S ASVHSHCR+TL
Sbjct: 3   SSSNPISLLLFFSLLFLFTSSSA--VPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTL 62

Query: 62  RNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQA 121
            +AL  A  F+ LVD  L+LFPSLSALHDCR LA ANLDFIS T   VN+T   LP+YQA
Sbjct: 63  HHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPFYQA 122

Query: 122 HEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVSED 181
            EM+SL+SA+ITN DTC+DGL G G  LG VDKV++AISFDR+LYSLYLSLFKMGWVS++
Sbjct: 123 DEMISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWVSKN 182

Query: 182 MKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILING 241
           MKAP LP  NHFG GKG LKLKMSPKDRAYYERLVH KKP AARRLLQTNY DDGIL+NG
Sbjct: 183 MKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKP-AARRLLQTNYADDGILVNG 242

Query: 242 MVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMMGE 301
           +VAVDQNGAYDFTTI+DAIAAAPNKS  A+GYFLIFVTAGIY ESV+VPK K+YVLM+GE
Sbjct: 243 VVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGE 302

Query: 302 GNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSADNV 361
           GNN+TIITGNKNV+DGSTTFNSATV VEG GFFG NLTI+NTAGA KHQAVALRVSAD  
Sbjct: 303 GNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMT 362

Query: 362 TLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 421
           T YSCIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP+SGQFNA
Sbjct: 363 TFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 422

Query: 422 LTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSFID 481
           LTAQGRTD NQNTG  IH CTIKPT +LAASP TKSYLGRPWKE+SRT+YMQSFID+FID
Sbjct: 423 LTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFID 482

Query: 482 PAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGDQW 541
           PAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS+RA W VGVINATVA+ FTVS+ LAGDQW
Sbjct: 483 PAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAGDQW 542

Query: 542 LPPTAVPYTGGLIS 556
           LPPTAVPY+GGLIS
Sbjct: 543 LPPTAVPYSGGLIS 553

BLAST of Clc02G01180 vs. ExPASy TrEMBL
Match: A0A6J1I6B8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.9e-254
Identity = 445/554 (80.32%), Postives = 486/554 (87.73%), Query Frame = 0

Query: 2   ASSNPISLLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFTL 61
           +SSNPISLLTF + LFL   +SS+ VPT E+CQS  SPSFC SFLP+S ASVHSHCR+TL
Sbjct: 3   SSSNPISLLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTL 62

Query: 62  RNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQA 121
            +AL  A  F+ LVD  L+L PS SALHDCR LA ANLDFIS T + VN+T   LP+YQA
Sbjct: 63  HHALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQA 122

Query: 122 HEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVSED 181
            EM+SL+SA+ITN DTC+DGL   GS LGLVDKV++AISFDR+LYSLYLSLFKMGWVS+ 
Sbjct: 123 DEMISLLSAVITNVDTCHDGLA--GSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKT 182

Query: 182 MKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDGILING 241
           MKAP LP  NHFG G G LKLKMSPKDRAYYERL+H KKP AARRLLQTN  DDGIL+NG
Sbjct: 183 MKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKP-AARRLLQTNNADDGILVNG 242

Query: 242 MVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMMGE 301
           +VAVDQNGAYDFTTI+DAIAAAPNKS  A+GYFLIFVTAGIY ESV+VPK K+YVLM+GE
Sbjct: 243 VVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGE 302

Query: 302 GNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSADNV 361
           GNN+TIITGNKNV+DGSTTFNSATV VEG GFFG NLTI+NTAGA KHQAVALRVSAD  
Sbjct: 303 GNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMT 362

Query: 362 TLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 421
           T YSCIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP+SGQFNA
Sbjct: 363 TFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 422

Query: 422 LTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYMQSFIDSFID 481
           LTAQGRTD NQNTG  IH CTIKPT +LAASP TKSYLGRPWKE+SRT+YMQSFID+FID
Sbjct: 423 LTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFID 482

Query: 482 PAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTVSQFLAGDQW 541
           PAGWKEWDGTLNLNTSYYAEF+NSGPGSDTSRRA W VGVINATVA+ FTVS+ LAGDQW
Sbjct: 483 PAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGDQW 542

Query: 542 LPPTAVPYTGGLIS 556
           LPPTAVPY+GGLIS
Sbjct: 543 LPPTAVPYSGGLIS 553

BLAST of Clc02G01180 vs. ExPASy TrEMBL
Match: A0A6J1L6X0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 6.9e-248
Identity = 436/563 (77.44%), Postives = 481/563 (85.44%), Query Frame = 0

Query: 1   MASSNPISLLTFLTFLFLLSSSSSAV---VPTAEICQSTASPSFCNSFLPNSAASVHSHC 60
           M SS+ I LLTFLT L+L S SSSA+   VP A+IC ST SPSFC +FLPNS A+VHSHC
Sbjct: 1   MGSSSAIPLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLRNALDHAHAFLALVDSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAM--- 120
           RFTLR+AL HA  FLALVD+HL+LFPSL+ALHDCR LA ANLDF+S TF  VN+T     
Sbjct: 61  RFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 -VLPYYQAHEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLF 180
             LP+Y+A EMVSL+SA+ITNE+TCY+GL   GSA GLVDKVL+AISFD KLYSLYLSLF
Sbjct: 121 NALPFYKAEEMVSLLSAVITNENTCYEGLMSSGSASGLVDKVLKAISFDTKLYSLYLSLF 180

Query: 181 KMGWVSEDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTN-Y 240
           KMGWVS D+KAP +P M HFG GKGQLKL MSPK+RAYYERL+HR KP  ARRLLQTN  
Sbjct: 181 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKP-PARRLLQTNSN 240

Query: 241 QDDGILINGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKY 300
           +DDGIL+NG+VAVDQNG YDFTTI+ AI AAPN S  ADGYFLI VTAG+YNESV+VPK+
Sbjct: 241 EDDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMVPKH 300

Query: 301 KRYVLMMGEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAV 360
           K+YVLM+GEGNNQTIITGNKNV+DGSTTFNS T  VEG GFFGVNLTI+NTAG  K QAV
Sbjct: 301 KKYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKFQAV 360

Query: 361 ALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYAR 420
           ALRVSAD  T YSCIFEGYQDTLY HSLRQFYRECDIYGT+DFI G+AAVV QNCNIYAR
Sbjct: 361 ALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYAR 420

Query: 421 LPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPATKSYLGRPWKEYSRTIYM 480
           LPM GQ+  +TAQGRTD NQNTG SIHNCTIKPT++LAASP  KSY GRPWKEY+RT+YM
Sbjct: 421 LPMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYM 480

Query: 481 QSFIDSFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWVVGVINATVASNFTV 540
           QSFID+FIDP GW  WDGTLNLNTSYYAEFNNSGPGSDTSRRA W VG+INATVA+NFTV
Sbjct: 481 QSFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNFTV 540

Query: 541 SQFLAGDQWLPPTAVPYTGGLIS 556
           S FL GDQWLPPT VPYTGGLIS
Sbjct: 541 SPFLGGDQWLPPTTVPYTGGLIS 562

BLAST of Clc02G01180 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 520.4 bits (1339), Expect = 1.8e-147
Identity = 286/570 (50.18%), Postives = 368/570 (64.56%), Query Frame = 0

Query: 13  LTFLFLLSSSSSAVVP--------------TAEICQSTASPSFCNSFLPNSAASVHSHCR 72
           L FLF L+  SS   P               + IC+    PS+C S LPN    ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 73  FTLRNALDHAHAFLALVDSHL------SLFPSLSALHDCRGLAEANLDFISQTFSIVNST 132
            +LR +L  A  F++++D+ L      +   ++ AL DC+ LA   +D++  +    +ST
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST 124

Query: 133 AMVLPYYQAHEMVSLMSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSL 192
              L   +A ++ + +SA ITNE TC +GL    S  GL   +      D KLY + L+L
Sbjct: 125 -KTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFN----DTKLYGVSLAL 184

Query: 193 FKMGWV-----SEDMKAPMLPNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRL 252
           F  GWV     S  +  P       FG   G+L LKM+ + RA Y  +  RK       L
Sbjct: 185 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRK-------L 244

Query: 253 LQTNYQDDGILINGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESV 312
           LQ++   D + ++ +V V QNG  +FTTI+ AIAAAPNK+  ++GYFLI+VTAG+Y E V
Sbjct: 245 LQSD--ADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYV 304

Query: 313 LVPKYKRYVLMMGEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAA 372
            VPK KRYV+M+G+G NQT+ITGN++V+DG TTFNSAT  + G  F GVN+TI NTAG  
Sbjct: 305 EVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPT 364

Query: 373 KHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNC 432
           K QAVALR   D    YSC FE YQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNC
Sbjct: 365 KGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNC 424

Query: 433 NIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPAT-KSYLGRPWKEY 492
           N+Y R P  GQ N +TAQGRTDPNQNTGT+IH CTI+P  +LA S  T K+YLGRPWKEY
Sbjct: 425 NLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEY 484

Query: 493 SRTIYMQSFIDSFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINAT 552
           SRT+ MQ++ID F++P+GW  W G   L+T YYAE+NN+GPGSDT+ R +W    VINAT
Sbjct: 485 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINAT 544

Query: 553 VASNFTVSQFLAGDQWLPPTAVPYTGGLIS 556
            ASNFTV+ FL G+ W+  T VP+ GGLI+
Sbjct: 545 DASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of Clc02G01180 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 503.1 bits (1294), Expect = 3.0e-142
Identity = 283/579 (48.88%), Postives = 371/579 (64.08%), Query Frame = 0

Query: 7   ISLLTFLTFLFL----LSSSSSAVVP---TAEICQSTASPSFCNSFLP-NSAASVHSHCR 66
           +SL  FL  LFL    L  +S  ++P   ++ IC++T  P FC S  P  S   V  + R
Sbjct: 2   LSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGR 61

Query: 67  FTLRNALDHAHAFLALVDSHLSLFPSL------SALHDCRGLAEANLDFISQTFSIVNST 126
           F+LR +L  +  F   +D +L    +L       AL DCR LA    D++  +F  VN T
Sbjct: 62  FSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNIT 121

Query: 127 --AMVLPYYQAHEMVSLMSAMITNEDTCYDGL-TGLGSALGLVDKVLEAISFDRKLYSLY 186
             +  L + +A E+ +L+SA +TNE TC DG+ T   S+  + + V   +  D KL+S+ 
Sbjct: 122 TSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVS 181

Query: 187 LSLFKMGWVSEDMKAPML-----PNMNHFGV------GKGQLKLKMSPKDRAYYERLVHR 246
           L+LF  GWV +  K         P   H           G L LKM+   RA YE L  R
Sbjct: 182 LALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRR 241

Query: 247 KKPAAARRLLQTNYQDDGILINGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGYFLIFV 306
           K       L   +   + +L++ +V V+QNG  +FTTI++A+ +APNK+    GYF+I+V
Sbjct: 242 K-------LADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYV 301

Query: 307 TAGIYNESVLVPKYKRYVLMMGEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGFFGVNL 366
           T+G+Y E+V++ K KRY++M+G+G N+T++TGN+NV+DG TTFNSAT AV    F  VN+
Sbjct: 302 TSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNM 361

Query: 367 TISNTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFG 426
           T  NTAG  KHQAVA+R SAD    YSC FE YQDTLYTHSLRQFYRECDIYGTVDFIFG
Sbjct: 362 TFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFG 421

Query: 427 NAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTSELAASPAT-KS 486
           NAAVV Q+CN+Y R PM  QFNA+TAQGRTDPNQNTG SIHNCTIKP  +L +S  T K+
Sbjct: 422 NAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKT 481

Query: 487 YLGRPWKEYSRTIYMQSFIDSFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASW 546
           YLGRPWKEYSRT++MQS+ID  ++P GW+EW+G   L+T YYAE+NN+G GS T+ R  W
Sbjct: 482 YLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVW 541

Query: 547 V-VGVINATVASNFTVSQFLAGDQWLPPTAVPYTGGLIS 556
               VIN+T A+NFTV  FL GD W+  + VPY  GL+S
Sbjct: 542 PGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of Clc02G01180 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 498.0 bits (1281), Expect = 9.5e-141
Identity = 283/584 (48.46%), Postives = 368/584 (63.01%), Query Frame = 0

Query: 9   LLTFLTFLFLLSSSSSAVVPTAEICQSTASPSFCNSFLPNSAASVHSHCRFTLRNALDHA 68
           L+T   FL  + +SS  +  ++ IC++T  P +C S  P+S  +V  +  F++R +L  +
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 69  HAFLALVD------SHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTA-----MVLP 128
             F+  VD      +HLS    + AL DCR LA   +D++  +F  VN T+       L 
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 129 YYQAHEMVSLMSAMITNEDTCYDGLT-----------GLGSALGLVDKVLEAISFDRKLY 188
           + +A ++ +L+SA +TNE TC +GLT             G AL LV+        D KL 
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVN--------DTKLL 187

Query: 189 SLYLSLFKMGWVSEDMK-------APMLPNMNHFG----VGKGQLKLKMSPKDRAYYERL 248
            + L+LF  GWV +  K        P   +  H         G L LKM+ K +A YE L
Sbjct: 188 GVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESL 247

Query: 249 VHRKKPAAARRLLQTNYQDDG--ILINGMVAVDQNGAYDFTTISDAIAAAPNKSAVADGY 308
             RK              DDG  +LI+ +V V Q+G  +FT I+ A+AAAPN +  + G+
Sbjct: 248 SRRKLADGD----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGF 307

Query: 309 FLIFVTAGIYNESVLVPKYKRYVLMMGEGNNQTIITGNKNVIDGSTTFNSATVAVEGIGF 368
           FLI+VTAGIY E + + K KRY++M+G+G NQT++TGN++V+DG TTFNSAT AV    F
Sbjct: 308 FLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNF 367

Query: 369 FGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEGYQDTLYTHSLRQFYRECDIYGTV 428
             VN+T  NTAG  KHQAVALR  AD    YSC FE YQDTLYTHSLRQFYRECD+YGTV
Sbjct: 368 VAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTV 427

Query: 429 DFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKPTSELAASP 488
           DFIFGNAAVV QNCN+Y R PM  QFNA+TAQGR+DPNQNTGTSI NCTIKP  +L +S 
Sbjct: 428 DFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN 487

Query: 489 AT-KSYLGRPWKEYSRTIYMQSFIDSFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTS 548
            T K+YLGRPWKEYSRT+YMQS+ID F++P GW+EW+G   L+T YYAE+NN+GPGS+T+
Sbjct: 488 YTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTT 547

Query: 549 RRASWV-VGVINATVASNFTVSQFLAGDQWLPPTAVPYTGGLIS 556
            R +W    VIN+T A+NFTV+       W+  T VPYT GLIS
Sbjct: 548 NRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of Clc02G01180 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 413.3 bits (1061), Expect = 3.1e-115
Identity = 234/547 (42.78%), Postives = 328/547 (59.96%), Query Frame = 0

Query: 33  CQSTASPSFCNSFL---PNSAASVHSHCRFTLRNALDHAHAFLALVDSHLSLFPS----- 92
           C+ST  P  C + L    +S +  + + +FT++  L  A     ++ S+     S     
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 93  ----LSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQAHEMVSLMSAMITNEDTCYD 152
               + A+ DC  L+E +++++ +T +    TA V+       + SL+S ++TN+ TC D
Sbjct: 143 TAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 153 GLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKAPMLPNMNHFGVGKGQL 212
           GL    S  G    +   +    +LYS+      +G VS  +      N+  F   KG++
Sbjct: 203 GLVEAKS--GFAAAIGSPMGNLTRLYSI-----SLGLVSHALNR----NLKRFKASKGKI 262

Query: 213 KLKMSPKDRAYYERLVHRKKPA---------AARRLLQTNYQDDG-ILINGMVAVDQNGA 272
               +   R   E L+   +            +R L +      G IL++  V V    +
Sbjct: 263 LGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 322

Query: 273 YDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMMGEGNNQTIITG 332
            +FTTI+DAIAAAPN +   DGYF+I+   G+Y E ++VP  K+ +++MG+G N+TIITG
Sbjct: 323 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 382

Query: 333 NKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYSCIFEG 392
           N NV+DG TT+N ++ AV G  F  V++T  NTAG  KHQAVALR +A+  + Y C FEG
Sbjct: 383 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 442

Query: 393 YQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDP 452
           YQDTLY HSLRQFYRECDIYGTVDFIFGNAA + QNCNIYAR PM+ Q NA+TA GR DP
Sbjct: 443 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 502

Query: 453 NQNTGTSIHNCTIKPTSELAASP-ATKSYLGRPWKEYSRTIYMQSFIDSFIDPAGWKEWD 512
           NQNTG SI NCTIK   +LAA P +  ++LGRPWK YSRT++MQS+I   + P GW EW+
Sbjct: 503 NQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWN 562

Query: 513 GTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVASNFTVSQFLAGDQWLPPTAVP 556
           GT+ L+T YY E++N GPG++T++R  W+   ++N   A NFTV  F  GD WLP T +P
Sbjct: 563 GTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIP 617

BLAST of Clc02G01180 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 399.4 bits (1025), Expect = 4.6e-111
Identity = 230/551 (41.74%), Postives = 321/551 (58.26%), Query Frame = 0

Query: 20  SSSSSAVVPTAEICQSTASPSFCNSFL---PNSAASVHSHCRFTLRNALDHAHAFLALV- 79
           S S  + +P    C+ST  P  C + L    +S +  + + +FT++  L  A     ++ 
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 80  --------DSHLSLFPSLSALHDCRGLAEANLDFISQTFSIVNSTAMVLPYYQAHEMVSL 139
                   D   S    +SA+ DC  LAE +++++ +T +     A ++       + SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSL 194

Query: 140 MSAMITNEDTCYDGLTGLGSALGLVDKVLEAISFDRKLYSLYLSLFKMGWVSEDMKAPML 199
           +  ++TN+ TC DGL    S  G    +   +    +LYS+ L L     ++ ++K    
Sbjct: 195 LGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKG 254

Query: 200 PNMNHFGVGKGQLKLKMSPKDRAYYERLVHRKKPAAARRLLQTNYQDDG--ILINGMVAV 259
                FG G   ++  +    +   +     K    A R L    +  G  IL+   V V
Sbjct: 255 SKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314

Query: 260 DQNGAYDFTTISDAIAAAPNKSAVADGYFLIFVTAGIYNESVLVPKYKRYVLMMGEGNNQ 319
                 +F TI++A+AAAPN +    GYF+I+  AG+Y E V++   KR ++++G+G N+
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374

Query: 320 TIITGNKNVIDGSTTFNSATVAVEGIGFFGVNLTISNTAGAAKHQAVALRVSADNVTLYS 379
           TII+GN + IDG TT+NS+T AV G  F  V++T  NTAG  KHQAVA+R +AD  T Y 
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434

Query: 380 CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 439
           C FEGYQDTLY HSLRQFYRECDIYGT+DFIFGNAA + QNCNIYAR PM+ Q NA+TA 
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494

Query: 440 GRTDPNQNTGTSIHNCTIKPTSELAASP-ATKSYLGRPWKEYSRTIYMQSFIDSFIDPAG 499
           GRTDPNQ TG SI NCTI    +LAA P +T ++LGRPWK YSRT+Y+QS+I   + P G
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 554

Query: 500 WKEWDGTLNLNTSYYAEFNNSGPGSDTSRRASWV-VGVINATVASNFTVSQFLAGDQWLP 555
           W EW+GT  L+T  Y E++N GPG+DTS+R  W    ++N   A NFTV  F  GD WLP
Sbjct: 555 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 614