Clc02G01160 (gene) Watermelon (cordophanus) v2

Overview
NameClc02G01160
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionGDSL esterase/lipase 1-like
LocationClcChr02: 1203904 .. 1209031 (-)
RNA-Seq ExpressionClc02G01160
SyntenyClc02G01160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGTCAGGGTCAAGTTGTCACTATTTGATTTTGGTTAATGTATATAACCCTAATTTGTATATCTCCACATTTTTCAGACGAAAAACTTTGTAAGCAACTTTTAGTAAATGCAATCACGTGACATCGTCAGTTTGTCACAGAAAGCTTCAATAATTGTTGTTATAAGAAGAGAGCAAGAGAAGTAGGCTCGTCAACAGCTCATAGTGAGAGATGAAGATTTCAAAATTGGGATTCTGCTTCTTCTTCTTCTCCATCATCGTCTTGTTTGGAAATGGTGCAGCCCAAAGCGGTTTCCCGGAAATCGAAATGCCCGATGTTGGGTGGTCGGAAAAGGGGTTCGGGTTTTTCATATTCGGAGATTCTTATGTGGATGGTGGAAACAACAACTACATCAACACCACCATCGACTCTCAGGCCAACTTTCCGCCCTATGGTGAAACCTTCTTCCACTTCCCAACCGGCCGGTTTTGTGACGGCCGCATTATACCTGATTTCTTAGGTCAGAACACTTCTTTTTCTTTTTTTCTTTCCTTTCAAATTTAGTTTCCATTAAAGTAGCCTTTTGCTTCTCCCAAACTTTCTTTTTTTTTTTTTTTAACTTGGCTTCATTCTTTATAGAGGAATGATAGGTAAAAAAATAAAATTAAATAGTTTTAAAAATTAAAAAATAAAAATAATGACTACTCTCGAATATAGCAAAATAATTAAACTATCATTTTTTTAAAATAGTTTAATATTTTTTCTATTTATAATAATTTTTCTAAAAATAATTCAGATTTTAATTTTTATTTTCTTTTTGCTAAATGGTAGAATTTTATTTGGCAATTGTTGAAAGCACCCCTTTTTTTTCTTTTAAAAATATTAGCAAAAAGTAGAAAATATAATATGAAAATTTTGAGGTTACTATAATAAGGAATTATAATAGTTTGTTTTTGGGGTGAAGACTATTTTAATATGGTTTATAATAATTCGAATTTTGGATGTAAACTATTTTTGTTTGGGTAAACCGTCATGTGTTGGTTGGGTTGGTGGTAAAAAAGAGACCTAGTTTCAATAAATGATTAAGAGGAAGGGTTCAATCAATGATGATTACTTACCTAAGAATTAATTTCATATCAGTTTTCTTGGCATCCAAATGTTGCTTATAATTGCATTATTTTCTTACTATAATTGCATCATTTTTAAGTATAAGAGTTGACTTTTTAACTTAATTCTAAAGATAAAAACAAGTTCTTAAAAACCTTTTTTTTTATTTATTATTATTATTTTAAATTTTGGCTTGATTTTTGAAAATGTGGGCAAAATGTAAATAACAAACAAAGCAAAAAAATTCAGAGGTGAAAAAAATGTTTATAAGCTTAATGTAGGGAATGACCGACAAGTACGAAAGGCTGTGGCTCATAGTAAGTTCACACTTAGAATTCTTTGTCCCACATTAGAATATCAAGGGGGTAAGAAGAAGTAAAAATATGAGAGGTCTCCACGAAATTTACAAAAGTGGTTAGAGTGGTATAGTTAAGGATCTTCTCCATGTGCATCAAGGCTTTAGATCTTGATTAGACATCAAATAACACTTCTTTTCCAAAAATATAAAAAATAAAAAACCAAAAATCAATTTATTTAATTTTTGTTTTTGGCAGCGGAGTATGCAGAACTACCTTTGATTGAAGCATATTTAGATCCTAAAAACAAAAACTATATGAATGGTGTGAATTTTGGATCTGGTGGAGGTGGAGCTTTAGCTGAGACTCATCAAGAACAGGTTCCAATCAATCTAATTTCTTTTCCTGTTTTTTTTTCTTCTGCTGAATTGAATGCATATAATTTTAGTTTGGTTTTACAGGCCATTGGGCTTCAAACTCAAATGAAGTTCTTCAAAAATGTGGAGAAATCATTAAGAAAGAAGCTGGGAAATGCAAGATCCCAAAGCTTCCTCTCTAACTCTGTTTTTCTCTTCAATTTTGGAGGAAATGATTACCAAAATCCCTTTGACATTAGCTATGACATCTTCAAAACCATTGAAGCTCAACAACAGTTCGTCAATCTTGTGGTCGGAAACATAACGATAGCTCTCAAGGTAATCTCTTTGCGTGTCTAAATGTGTTAGGTTAAATTATACAAAATTAACCTTTAATACGTTTAAAGGTGGATGCGCTATAGCAACACGAAAAAATAATAATACAAGCACTAAGTGACCTTACAACTAAAATGTTTCAACAAAAGTTGTATGTTAATAGTTGAGTTGTCAGTTAACTTACGTTTGGGTTAAATACATAGGAATGAAAAACAAATAAGATTTAGTTCATCTTTTTGCAATTCAAAATAGTATATTTATTTTTTGTGGTCCGTTAGGAAGTTTCCTCATTTTCGAAAATGATATTTCTTTTTTGTCATTTTGTATTTGGTAATTCATCATCTTGTGAAATTTTGAGGGAAGATTTTAATTTTTGGAATAACATTATGAGATTTTATTCTTTAACTTGAAGTTTTGGGTCTTGCAATGGGTAGCACTTATAGGATTAATAATTAAGTGTATAACAACATTTTTAAAAAGCAAAATCTACTAGAGAGTCGGATTTAAAATTGTTTGGGGAGTAATTTTTTATTGCTCAAAATGAGCATAGTTGAATTTGACATAGTGTTTTCATATGTCAACCTCGAGAGTAGAGATAGAGATGGAGAGTGAATGAAAAATATTTTTGTAGGTTTTCGAATGAGATGGGATTGATGTGATATAGGAATTATACAAATTTGGAGGAAGGAAATTTGGGTTCATGACGGTGCCTCCCTTGGGTTATCTGCCAAGTGCAAGATTGAAAAGAAGTGAGCAATTTTTCCAAGAGACTTCTTCATTATCAAGGATTCACAACAAAGAGCTTTTCATTGCCATACAAAGACTTGCCACTCAACTCAAGGGATTCAAATTTGCTTTCGCTGATACTCACACTTTGCTTCTACAAAGAATACAAAACCCTACCAAATATGGTAACCAACTTAATTTCTTCACCCTATTTCTTTTTTATAATCAACACATTTGGTCATATATATAAGGTTCAAAAAACACTTTTTACTACTTAAACTTCAATGAATATAACATCTTTTAGGATAGGAACTACAATTGTATTGTAACAGATTAAATTTGAGATTGTCCTAATCTGAACTTTTTGATAATGTGGATGAAGGTTTCAAGGTAGCGGACACAGCATGCTGTGGGAGTGATGATTTTAGAGGGTATTATAATTGTGGAAGAAAGAAAGGGACAGTGCCATATACTCACTGCAAAAATATACAAGACTATTTGTTCTATGATTCTTATCATCCCACTGAAAAGGCATTCGAGCAACTAGCAAAACTGATGTGGAGTGGAGGTGATGAAGTTGTTAAGCCTTATAACTTCAAACAACTCTTTCAATATGACTCAACCTTGGCGTCTGATTGACAAAGAGATGTGATGAAGATGTTATACTTTTTTCCTCTCTTTTTTGGAACACTTACAATAGTGTTAAGTTCGAGAATTCTTACCTCTGTATTTTGAAAACCCTTGATGAATGAAATCCTTGCTGCTGCTTTCTTTAGAGTAGATGTAGGTGAATCACCATAATACTTTTGTGTTCTTCTTCCCTTTTTGTGTTTTTCATCTTGATATAATTTCATCTTTTCTCTGTGTATTTTGCTTCTCTGTATGAAAGAAGCAAAATGAGTGAAGTTAGTCTTGATGGGTTTGCATGTGGTGTGACAAGTGCTGGAAAATGTGTAAGAATATAAAGAAAAGGTAAAGTGGGACTTGACAGCAAATAACCCATCTTTTCTATTAACAATTTTCAGAGCAAGCAACGATCAAAGAAAGCATCAAGAAAGGTTAAAGAAGCTAGTTGTTGAAAATGGCAACAACTAGATATAGAGATTGAGAAGTTTAGTGGCAATGGAGACTTTGGTCTGTAGTCAATTCTATTTTAGCAGAAGGCTTTCAAGGCCCTTGATGATCCAAAAACACTACCAGCCACAATTACGGCAGAAGAAAAATAGAAAATGGAAGAAGCAACCTACAGTGCATTAATTTTAAATATTTTTTATAGTGTACTTAGAAAAATTGAAGATGAAGATACAACATAAGCGATATGGACAAAATTGCAATCTTTATACATCAAGAAAGATGTTCCTAACAAGATTTACATTCGAGAAAAGCTGTTTACTTACTGTATGAATAGTTCAAAGTCCCTTGATGAGTTCAAGAGACTCACTAAAGAGTTTGCTACTATAGGGGACAAACTGGGTTCAGAAAGTGAAGCTACTCTTCTAATAAACTCCTTACCTGAAGCCTACAAAGATATGAAGATTGCCTTGAACTATGAAAAAGATTCTATTAATTTGGAATCTGTTATTTCAGCAATTAAATGCAAAGAGTTAGAGTTGAAACAAAAAAGTAAAGGAAATGACTTTTTTCTCAAAAGGTGTTACCCGAATTCGCAAAAATGTGTCCACACCCTCCAAACTGACTTGACGAGGAATCCATTCTCGCGAACAAACACAACCCCTACACACACCTAGGAACACCCCTTCATGCATATTCATACAAGACTTGAGTAGGCAGGTCGAGGTCTTACGAAGTACTCACTACTCAGAGTACCTTCTCAAAAGGAAAAAGATGTGTCTGTCAGGTTCGGTTCTTCTTAGTTGGGTTGGGTGAGTTCGACCAGAAATGCTATGCTTACATGCAAGACTACCTATCTTCCCAAAAGCTTTGCGAGGACTACTTTACGACGATGGACGACCTCCCGCAAGAGGTTTACTAAACAAGGTCCCGGCAGAGGATAAACTCAAACCCAGTGAATAGAAGATGCTTATTTCCGCACAACATAGGGATTGGCATGCTTTTGGAAAGAAATAGAATAGTAGAGGATCCAACACGTGAAACACGACGATCATTAGAAGTTCACGAATTAGAGATGTTGTAATATACTCTTTCCCATAACTTCTCATACTATAGCAACCCACTAAAATCCAAATATGGATAAAAAATGTGGTCAATTTCACGAAGATAAAAGATATGGATTTGACAGCACATAACCTATTTTACAAAGTTGATGAAATACTTTGGTAATATCATCCATTAGCTTTGTCATGGCA

mRNA sequence

TGGGTCAGGGTCAAGTTGTCACTATTTGATTTTGGTTAATGTATATAACCCTAATTTGTATATCTCCACATTTTTCAGACGAAAAACTTTGTAAGCAACTTTTAGTAAATGCAATCACGTGACATCGTCAGTTTGTCACAGAAAGCTTCAATAATTGTTGTTATAAGAAGAGAGCAAGAGAAGTAGGCTCGTCAACAGCTCATAGTGAGAGATGAAGATTTCAAAATTGGGATTCTGCTTCTTCTTCTTCTCCATCATCGTCTTGTTTGGAAATGGTGCAGCCCAAAGCGGTTTCCCGGAAATCGAAATGCCCGATGTTGGGTGGTCGGAAAAGGGGTTCGGGTTTTTCATATTCGGAGATTCTTATGTGGATGGTGGAAACAACAACTACATCAACACCACCATCGACTCTCAGGCCAACTTTCCGCCCTATGGTGAAACCTTCTTCCACTTCCCAACCGGCCGGTTTTGTGACGGCCGCATTATACCTGATTTCTTAGCGGAGTATGCAGAACTACCTTTGATTGAAGCATATTTAGATCCTAAAAACAAAAACTATATGAATGGTGTGAATTTTGGATCTGGTGGAGGTGGAGCTTTAGCTGAGACTCATCAAGAACAGGTTCCAATCAATCTAATTTCTTTTCCTGTTTTTTTTTCTTCTGCTGAATTGAATGCATATAATTTTAGTTTGGTTTTACAGGCCATTGGGCTTCAAACTCAAATGAAGTTCTTCAAAAATGTGGAGAAATCATTAAGAAAGAAGCTGGGAAATGCAAGATCCCAAAGCTTCCTCTCTAACTCTGTTTTTCTCTTCAATTTTGGAGGAAATGATTACCAAAATCCCTTTGACATTAGCTATGACATCTTCAAAACCATTGAAGCTCAACAACAGTTCGTCAATCTTGTGGTCGGAAACATAACGATAGCTCTCAAGGAATTATACAAATTTGGAGGAAGGAAATTTGGGTTCATGACGGTGCCTCCCTTGGGTTATCTGCCAAGTGCAAGATTGAAAAGAAGTGAGCAATTTTTCCAAGAGACTTCTTCATTATCAAGGATTCACAACAAAGAGCTTTTCATTGCCATACAAAGACTTGCCACTCAACTCAAGGGATTCAAATTTGCTTTCGCTGATACTCACACTTTGCTTCTACAAAGAATACAAAACCCTACCAAATATGGTTTCAAGGTAGCGGACACAGCATGCTGTGGGAGTGATGATTTTAGAGGGTATTATAATTGTGGAAGAAAGAAAGGGACAGTGCCATATACTCACTGCAAAAATATACAAGACTATTTGTTCTATGATTCTTATCATCCCACTGAAAAGGCATTCGAGCAACTAGCAAAACTGATGTGGAGTGGAGGTGATGAAGTTGTTAAGCCTTATAACTTCAAACAACTCTTTCAATATGACTCAACCTTGGCGTCTGATTGACAAAGAGATGTGATGAAGATGTTATACTTTTTTCCTCTCTTTTTTGGAACACTTACAATAGTGTTAAGTTCGAGAATTCTTACCTCTGTATTTTGAAAACCCTTGATGAATGAAATCCTTGCTGCTGCTTTCTTTAGAGTAGATAGCAAGCAACGATCAAAGAAAGCATCAAGAAAGGTTAAAGAAGCTAGTTGTTGAAAATGGCAACAACTAGATATAGAGATTGAGAAGTTTAGTGGCAATGGAGACTTTGGTCTGTAGTCAATTCTATTTTAGCAGAAGGCTTTCAAGGCCCTTGATGATCCAAAAACACTACCAGCCACAATTACGGCAGAAGAAAAATAGAAAATGGAAGAAGCAACCTACAGTGCATTAATTTTAAATATTTTTTATAGTGTACTTAGAAAAATTGAAGATGAAGATACAACATAAGCGATATGGACAAAATTGCAATCTTTATACATCAAGAAAGATGTTCCTAACAAGATTTACATTCGAGAAAAGCTGTTTACTTACTGTATGAATAGTTCAAAGTCCCTTGATGAGTTCAAGAGACTCACTAAAGAGTTTGCTACTATAGGGGACAAACTGGGTTCAGAAAGTGAAGCTACTCTTCTAATAAACTCCTTACCTGAAGCCTACAAAGATATGAAGATTGCCTTGAACTATGAAAAAGATTCTATTAATTTGGAATCTGTTATTTCAGCAATTAAATGCAAAGAGTTAGAGTTGAAACAAAAAAGTAAAGGAAATGACTTTTTTCTCAAAAGGTGTTACCCGAATTCGCAAAAATGTGTCCACACCCTCCAAACTGACTTGACGAGGAATCCATTCTCGCGAACAAACACAACCCCTACACACACCTAGGAACACCCCTTCATGCATATTCATACAAGACTTGAGTAGGCAGGTCGAGGTCTTACGAAGTACTCACTACTCAGAGTACCTTCTCAAAAGGAAAAAGATGTGTCTGTCAGGTTCGGTTCTTCTTAGTTGGGTTGGGTGAGTTCGACCAGAAATGCTATGCTTACATGCAAGACTACCTATCTTCCCAAAAGCTTTGCGAGGACTACTTTACGACGATGGACGACCTCCCGCAAGAGGTTTACTAAACAAGGTCCCGGCAGAGGATAAACTCAAACCCAGTGAATAGAAGATGCTTATTTCCGCACAACATAGGGATTGGCATGCTTTTGGAAAGAAATAGAATAGTAGAGGATCCAACACGTGAAACACGACGATCATTAGAAGTTCACGAATTAGAGATGTTGTAATATACTCTTTCCCATAACTTCTCATACTATAGCAACCCACTAAAATCCAAATATGGATAAAAAATGTGGTCAATTTCACGAAGATAAAAGATATGGATTTGACAGCACATAACCTATTTTACAAAGTTGATGAAATACTTTGGTAATATCATCCATTAGCTTTGTCATGGCA

Coding sequence (CDS)

ATGAAGATTTCAAAATTGGGATTCTGCTTCTTCTTCTTCTCCATCATCGTCTTGTTTGGAAATGGTGCAGCCCAAAGCGGTTTCCCGGAAATCGAAATGCCCGATGTTGGGTGGTCGGAAAAGGGGTTCGGGTTTTTCATATTCGGAGATTCTTATGTGGATGGTGGAAACAACAACTACATCAACACCACCATCGACTCTCAGGCCAACTTTCCGCCCTATGGTGAAACCTTCTTCCACTTCCCAACCGGCCGGTTTTGTGACGGCCGCATTATACCTGATTTCTTAGCGGAGTATGCAGAACTACCTTTGATTGAAGCATATTTAGATCCTAAAAACAAAAACTATATGAATGGTGTGAATTTTGGATCTGGTGGAGGTGGAGCTTTAGCTGAGACTCATCAAGAACAGGTTCCAATCAATCTAATTTCTTTTCCTGTTTTTTTTTCTTCTGCTGAATTGAATGCATATAATTTTAGTTTGGTTTTACAGGCCATTGGGCTTCAAACTCAAATGAAGTTCTTCAAAAATGTGGAGAAATCATTAAGAAAGAAGCTGGGAAATGCAAGATCCCAAAGCTTCCTCTCTAACTCTGTTTTTCTCTTCAATTTTGGAGGAAATGATTACCAAAATCCCTTTGACATTAGCTATGACATCTTCAAAACCATTGAAGCTCAACAACAGTTCGTCAATCTTGTGGTCGGAAACATAACGATAGCTCTCAAGGAATTATACAAATTTGGAGGAAGGAAATTTGGGTTCATGACGGTGCCTCCCTTGGGTTATCTGCCAAGTGCAAGATTGAAAAGAAGTGAGCAATTTTTCCAAGAGACTTCTTCATTATCAAGGATTCACAACAAAGAGCTTTTCATTGCCATACAAAGACTTGCCACTCAACTCAAGGGATTCAAATTTGCTTTCGCTGATACTCACACTTTGCTTCTACAAAGAATACAAAACCCTACCAAATATGGTTTCAAGGTAGCGGACACAGCATGCTGTGGGAGTGATGATTTTAGAGGGTATTATAATTGTGGAAGAAAGAAAGGGACAGTGCCATATACTCACTGCAAAAATATACAAGACTATTTGTTCTATGATTCTTATCATCCCACTGAAAAGGCATTCGAGCAACTAGCAAAACTGATGTGGAGTGGAGGTGATGAAGTTGTTAAGCCTTATAACTTCAAACAACTCTTTCAATATGACTCAACCTTGGCGTCTGATTGA

Protein sequence

MKISKLGFCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTLASD
Homology
BLAST of Clc02G01160 vs. NCBI nr
Match: XP_022927996.1 (GDSL esterase/lipase 1-like [Cucurbita moschata])

HSP 1 Score: 592.0 bits (1525), Expect = 3.8e-165
Identity = 290/408 (71.08%), Postives = 325/408 (79.66%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNY 60
           MKISK+G  FFFF   +L G  AA SGFP I++ D+  SE   GFFIFGDSYVD GNNNY
Sbjct: 1   MKISKMGSFFFFF--FILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNY 60

Query: 61  INTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYMNGV 120
           INTT D  ANFPPYGETFF  PTGRF DGRIIPDFLAEYA LPLI+ YLDPKN  Y+NGV
Sbjct: 61  INTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGV 120

Query: 121 NFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEK 180
           NF SGGGGALA +HQ                            QAIGLQTQMKFFK V+ 
Sbjct: 121 NFASGGGGALAGSHQG---------------------------QAIGLQTQMKFFKRVKN 180

Query: 181 SLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIA 240
           SL K+LGNAR+QSF SNSVFLFNFGGNDY NPFDISYDIFKT++AQ+QF ++V+GNITIA
Sbjct: 181 SLTKRLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIA 240

Query: 241 LKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLATQL 300
           LKE+YK GGRKFGFMTVPPLGY PS+RLK++ QFF ETSSL+R+HNK   +AIQRLATQL
Sbjct: 241 LKEVYKCGGRKFGFMTVPPLGYTPSSRLKKNAQFFDETSSLARVHNKLFLMAIQRLATQL 300

Query: 301 KGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNI 360
            GFK+AFADTHTLLLQRI NPTKYGFK+ DTACCGSD+FRG++NCGRKKG +PYT C+N+
Sbjct: 301 DGFKYAFADTHTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKGAIPYTLCENV 360

Query: 361 QDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTLAS 409
           QDY+F+DSYHPTEK FEQLAK MWSG   +VKPYNFKQLFQYD  L S
Sbjct: 361 QDYMFFDSYHPTEKTFEQLAKEMWSGDAHIVKPYNFKQLFQYDPFLGS 379

BLAST of Clc02G01160 vs. NCBI nr
Match: XP_038901400.1 (GDSL lipase-like isoform X3 [Benincasa hispida])

HSP 1 Score: 589.7 bits (1519), Expect = 1.9e-164
Identity = 285/409 (69.68%), Postives = 325/409 (79.46%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNY 60
           MKIS LG C FFF I +LFG  A QS F +I++P V    K FGFFIFGDSYVD GNNNY
Sbjct: 1   MKISNLGICVFFFYIFILFGIAAPQSAFLKIQLPHVQSWGKRFGFFIFGDSYVDAGNNNY 60

Query: 61  INTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYMNGV 120
           IN T D +ANFPPYGETFF  PTGRF DGRIIPDFLAEYA++P I+AYLDP N NY NGV
Sbjct: 61  INLTSDCRANFPPYGETFFPIPTGRFSDGRIIPDFLAEYAQMPFIQAYLDPNNNNYTNGV 120

Query: 121 NFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEK 180
           NF SGG GAL ET+Q+                           +AIGLQTQM FFK V+K
Sbjct: 121 NFASGGAGALVETNQK---------------------------KAIGLQTQMMFFKRVKK 180

Query: 181 SLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIA 240
           SLRKKLGN RSQSFLSNSVFLF+FGGNDY NP DI+YDIFKTIEAQ+QFVN+V+GNITIA
Sbjct: 181 SLRKKLGNERSQSFLSNSVFLFHFGGNDYLNPLDINYDIFKTIEAQEQFVNMVIGNITIA 240

Query: 241 LKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLATQL 300
           LKE+Y +GGRKFG M +PP GYLP++R KRSEQ+FQE+SS++RIHN  LFIA+Q+LATQL
Sbjct: 241 LKEVYNYGGRKFGIMALPPTGYLPNSRWKRSEQYFQESSSITRIHNNLLFIALQKLATQL 300

Query: 301 KGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNI 360
            GFK+AFADTHT LLQ I NPTK+GFKV DTACCGS +FRG Y+CGRK+  +P+THC N+
Sbjct: 301 NGFKYAFADTHTFLLQTILNPTKFGFKVVDTACCGSGEFRGIYSCGRKEEPLPFTHCTNL 360

Query: 361 QDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTLASD 410
           QDYLFYDSYHPTEKA EQLAKL+W+G DE+VKPYNFKQLFQYDS +  D
Sbjct: 361 QDYLFYDSYHPTEKASEQLAKLIWNGDDEIVKPYNFKQLFQYDSNMTFD 382

BLAST of Clc02G01160 vs. NCBI nr
Match: XP_022971691.1 (GDSL esterase/lipase 1-like [Cucurbita maxima])

HSP 1 Score: 589.3 bits (1518), Expect = 2.5e-164
Identity = 288/406 (70.94%), Postives = 326/406 (80.30%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNY 60
           MKISK+G  FFFF   +L GN AA SGFP I++PD+  SE   GFFIFGDSYVD GNNNY
Sbjct: 1   MKISKMGSFFFFF---ILLGNAAAGSGFPGIQLPDIPSSENRSGFFIFGDSYVDAGNNNY 60

Query: 61  INTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYMNGV 120
           INTT D  AN+PPYGETFF  PTGRF DGRIIPDFLAEYA+LPLI+ YLDPKN  Y+NGV
Sbjct: 61  INTTGDFLANYPPYGETFFTTPTGRFSDGRIIPDFLAEYAKLPLIQPYLDPKNNLYLNGV 120

Query: 121 NFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEK 180
           NF SGGGGALAE+HQ                            QAIGLQTQMKFFK V+ 
Sbjct: 121 NFASGGGGALAESHQG---------------------------QAIGLQTQMKFFKRVKN 180

Query: 181 SLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIA 240
           SL K+LG+AR++SF SNSVFLFNFGGNDY NPFDISYDIFKT++AQ+QF N+V+GNITIA
Sbjct: 181 SLTKRLGSARTRSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFANMVIGNITIA 240

Query: 241 LKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLATQL 300
           LKE+YK GGRKFGFMTVPP GY PS+RLK+S QFF ETSSL+R+HNK   +AIQRLATQL
Sbjct: 241 LKEVYKCGGRKFGFMTVPPFGYTPSSRLKKSAQFFYETSSLARVHNKLFLMAIQRLATQL 300

Query: 301 KGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNI 360
            GFK+AFADTHTLLL+RI NPTKYGFK+ DTACCGSD+FRG++NCGRKKG +PYT C+NI
Sbjct: 301 HGFKYAFADTHTLLLRRILNPTKYGFKIIDTACCGSDEFRGFFNCGRKKGALPYTLCENI 360

Query: 361 QDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTL 407
           QDY+F+DSYHPTEK FEQLA  MWSG  ++VK YNFKQLFQYD  L
Sbjct: 361 QDYMFFDSYHPTEKTFEQLANEMWSGDADIVKSYNFKQLFQYDPFL 376

BLAST of Clc02G01160 vs. NCBI nr
Match: XP_023512753.1 (GDSL esterase/lipase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582.4 bits (1500), Expect = 3.0e-162
Identity = 280/399 (70.18%), Postives = 320/399 (80.20%), Query Frame = 0

Query: 10  FFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNYINTTIDSQA 69
           FFFF + +L G  AA+SGFP I++P +  SE   GFFIFGDSYVD GNNNYINTT D  A
Sbjct: 4   FFFFFVFILLGIAAAESGFPGIQLPGIPSSENRSGFFIFGDSYVDAGNNNYINTTGDFLA 63

Query: 70  NFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYMNGVNFGSGGGGA 129
           N+PPYGETFF  PTGRF DGRIIPDFLAEYA LPLI+ YLDP N  Y+NGVNF SGGGGA
Sbjct: 64  NYPPYGETFFPTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPTNNLYLNGVNFASGGGGA 123

Query: 130 LAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEKSLRKKLGNA 189
           LA +H+                            QAIGLQTQMKFFK V+ SL K+LGNA
Sbjct: 124 LAGSHKG---------------------------QAIGLQTQMKFFKRVKNSLTKRLGNA 183

Query: 190 RSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIALKELYKFGG 249
           R+QSF SNSVFLFNFGGNDY NPFDISYDIFKT++AQ+QF ++V+GNITIALKE+YK GG
Sbjct: 184 RTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGG 243

Query: 250 RKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLATQLKGFKFAFAD 309
           RKFGFMTVPPLGY PS+RLK++ QFF E+SSL+R+HNK   +AIQRLATQL GFK+AFAD
Sbjct: 244 RKFGFMTVPPLGYTPSSRLKKNAQFFDESSSLARVHNKLFLMAIQRLATQLDGFKYAFAD 303

Query: 310 THTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNIQDYLFYDSY 369
           THTLLLQRI NPTKYGFK+ DTACCGSD+FRG++NCGRKKG +PYT C+N+QDY+F+DSY
Sbjct: 304 THTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKGALPYTLCENVQDYMFFDSY 363

Query: 370 HPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTLAS 409
           HPTEK FEQLAK MWSG  ++VKPYNFKQLFQYD  L S
Sbjct: 364 HPTEKTFEQLAKEMWSGDADIVKPYNFKQLFQYDPFLGS 375

BLAST of Clc02G01160 vs. NCBI nr
Match: XP_038901398.1 (GDSL lipase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 575.1 bits (1481), Expect = 4.8e-160
Identity = 285/436 (65.37%), Postives = 325/436 (74.54%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNY 60
           MKIS LG C FFF I +LFG  A QS F +I++P V    K FGFFIFGDSYVD GNNNY
Sbjct: 1   MKISNLGICVFFFYIFILFGIAAPQSAFLKIQLPHVQSWGKRFGFFIFGDSYVDAGNNNY 60

Query: 61  INTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYMNGV 120
           IN T D +ANFPPYGETFF  PTGRF DGRIIPDFLAEYA++P I+AYLDP N NY NGV
Sbjct: 61  INLTSDCRANFPPYGETFFPIPTGRFSDGRIIPDFLAEYAQMPFIQAYLDPNNNNYTNGV 120

Query: 121 NFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEK 180
           NF SGG GAL ET+Q+                           +AIGLQTQM FFK V+K
Sbjct: 121 NFASGGAGALVETNQK---------------------------KAIGLQTQMMFFKRVKK 180

Query: 181 SLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIA 240
           SLRKKLGN RSQSFLSNSVFLF+FGGNDY NP DI+YDIFKTIEAQ+QFVN+V+GNITIA
Sbjct: 181 SLRKKLGNERSQSFLSNSVFLFHFGGNDYLNPLDINYDIFKTIEAQEQFVNMVIGNITIA 240

Query: 241 LKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLATQL 300
           LKE+Y +GGRKFG M +PP GYLP++R KRSEQ+FQE+SS++RIHN  LFIA+Q+LATQL
Sbjct: 241 LKEVYNYGGRKFGIMALPPTGYLPNSRWKRSEQYFQESSSITRIHNNLLFIALQKLATQL 300

Query: 301 KGFKFAFADTHTLLLQRIQNPTKYG---------------------------FKVADTAC 360
            GFK+AFADTHT LLQ I NPTK+G                           FKV DTAC
Sbjct: 301 NGFKYAFADTHTFLLQTILNPTKFGESTTRLVGVVCGRPLTKPFENTVKLFCFKVVDTAC 360

Query: 361 CGSDDFRGYYNCGRKKGTVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKP 410
           CGS +FRG Y+CGRK+  +P+THC N+QDYLFYDSYHPTEKA EQLAKL+W+G DE+VKP
Sbjct: 361 CGSGEFRGIYSCGRKEEPLPFTHCTNLQDYLFYDSYHPTEKASEQLAKLIWNGDDEIVKP 409

BLAST of Clc02G01160 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.9e-80
Identity = 155/364 (42.58%), Postives = 222/364 (60.99%), Query Frame = 0

Query: 39  SEKGFGFFIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAE 98
           S++    FIFGDS  D GNNN+INT ++ +ANF PYG+++F  PTGRF DGRIIPDF+AE
Sbjct: 28  SQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDGRIIPDFIAE 87

Query: 99  YAELPLIEAYLDPKNKNYMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYN 158
           YA LP+I AYL+P N ++ +G NF S G GAL  +H                        
Sbjct: 88  YASLPIIPAYLEP-NNDFTHGANFASAGAGALIASHAG---------------------- 147

Query: 159 FSLVLQAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYD 218
                 A+GLQTQ+++F ++    R+ LG+ +S+  LS++V+LF+ GGNDYQ+P+   Y 
Sbjct: 148 -----LAVGLQTQLRYFGDLVDHYRQNLGDIKSRQLLSDAVYLFSCGGNDYQSPY---YP 207

Query: 219 IFKTIEAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKR-SEQFFQE 278
                  Q+Q+V++V+GN+T  +K +Y+ GGRKFG + VP +G  P  R K+       E
Sbjct: 208 Y-----TQEQYVDIVIGNMTNVIKGIYEKGGRKFGVVNVPLIGCWPGMRAKQPGNTCNTE 267

Query: 279 TSSLSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSD 338
              L+R+HN+     +++L  QL+GF +A  D  T +L R++NP+KYGFK  ++ACCGS 
Sbjct: 268 VDELTRLHNQAFAKRLEQLEKQLEGFVYAKFDLSTAILNRMKNPSKYGFKEGESACCGSG 327

Query: 339 DFRGYYNCGRKKGTVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFK 398
            F G Y+CGR K    +  C N  +Y F+D +HP E A  Q A++ W G   V +PYN K
Sbjct: 328 PFGGNYDCGRIK---EFGLCDNATEYFFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLK 352

Query: 399 QLFQ 402
            LF+
Sbjct: 388 ALFE 352

BLAST of Clc02G01160 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 297.0 bits (759), Expect = 3.3e-79
Identity = 162/402 (40.30%), Postives = 227/402 (56.47%), Query Frame = 0

Query: 8   FCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNYINTTIDS 67
           F FF  S  +LF  G + +                   F+FGDS++D GNNNYINTT   
Sbjct: 18  FIFFIVSSTILFLAGKSSAKISH------NGDNNVTALFLFGDSFLDAGNNNYINTTTLD 77

Query: 68  QANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKN-KNYMNGVNFGSGG 127
           QANFPPYG+TFF  PTGRF DGR+I DF+AEYA LPLI  +L+P N +  + GVNF S G
Sbjct: 78  QANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAG 137

Query: 128 GGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEKSLRKKL 187
            GAL ET Q  V                           I L+TQ+  +K VE+  R   
Sbjct: 138 AGALVETFQGSV---------------------------INLRTQLDHYKKVERLWRTNF 197

Query: 188 GNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIALKELYK 247
           G   S+  +S +V+L + G NDY + F  +  +     +  Q V++V+GN+T  + E+YK
Sbjct: 198 GKEESKKRISRAVYLISIGSNDYSSIFLTNQSL---PISMSQHVDIVIGNLTTFIHEIYK 257

Query: 248 FGGRKFGFMTVPPLGYLPSARL---KRSEQFFQETSSLSRIHNKELFIAIQRLATQLKGF 307
            GGRKFGF+ VP LG  P+ R+   K  +   ++ S L+ +HN+ L   + ++  Q+KGF
Sbjct: 258 IGGRKFGFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGF 317

Query: 308 KFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNIQDY 367
           KF+  D +  L  R+Q+P+K+GFK  + ACCG+  +RG ++CG K+    Y  C+N +DY
Sbjct: 318 KFSLFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDY 377

Query: 368 LFYDSYHPTEKAFEQLAKLMWSGG----DEVVKPYNFKQLFQ 402
           +F+DS H T+  + Q A L+W+GG      VV PYN   LFQ
Sbjct: 378 IFWDSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383

BLAST of Clc02G01160 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 9.7e-79
Identity = 157/362 (43.37%), Postives = 213/362 (58.84%), Query Frame = 0

Query: 46  FIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLI 105
           F+FGDS  D GNNNYI+T    ++N+ PYG+T F FPTGR  DGR IPDF+AEYA LPLI
Sbjct: 41  FVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLI 100

Query: 106 EAYLDPKN--KNYMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVL 165
            AYL P N    +  GV+F S G GAL  T    V                         
Sbjct: 101 PAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMV------------------------- 160

Query: 166 QAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTI 225
             I L++Q+  FK VEK LR  LG A+ +  +S +V+LF+ G NDYQ PF  +  IF++ 
Sbjct: 161 --INLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS- 220

Query: 226 EAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQF---FQETSS 285
             Q+ +V+ VVGN T  +KE+YK GGRKFGF+ +      P++ +    +    F+  + 
Sbjct: 221 SPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTE 280

Query: 286 LSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFR 345
           L  +HN++L   ++RL  +L GFK+A  D HT L  R+ NP+KYGFK    ACCG+   R
Sbjct: 281 LINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLR 340

Query: 346 GYYNCGRKKG-TVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQL 402
           G   CG + G +  Y  C+ + DYLF+D +H TEKA +Q+A+L+WSG   V KPYN + L
Sbjct: 341 GINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQAL 374

BLAST of Clc02G01160 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.7e-78
Identity = 158/362 (43.65%), Postives = 216/362 (59.67%), Query Frame = 0

Query: 46  FIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLI 105
           F+FGDS  D GNNNYI+T    ++N+ PYG+T F  PTGR  DGR+IPDF+AEYA LPLI
Sbjct: 39  FVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLI 98

Query: 106 EAYLDPKNKN--YMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVL 165
              L P N N  +  GVNF SGG GAL  T               FS   +N        
Sbjct: 99  PPNLQPFNGNSQFAYGVNFASGGAGALVGT---------------FSGLVIN-------- 158

Query: 166 QAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTI 225
               L+TQ+  FK VE+ LR KLG+A  +  +S +V+LF+ G NDYQ PF  +  +F++I
Sbjct: 159 ----LRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI 218

Query: 226 EAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARL---KRSEQFFQETSS 285
            + +++V+ VVGN+T   KE+Y  GGRKFG +   P    P++ +    +    FQ  + 
Sbjct: 219 -SNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTE 278

Query: 286 LSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFR 345
           L  +HN++L   ++RL  +L GFK+A  D HT L +R+ +P+KYGFK    ACCGS   R
Sbjct: 279 LINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLR 338

Query: 346 GYYNCGRKKG-TVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQL 402
           G   CG + G +  Y  C+N+ DYLF+D +H TEKA  Q+A+L+WSG   +  PYN K L
Sbjct: 339 GINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKAL 372

BLAST of Clc02G01160 vs. ExPASy Swiss-Prot
Match: Q9LJP1 (GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2)

HSP 1 Score: 282.3 bits (721), Expect = 8.5e-75
Identity = 154/362 (42.54%), Postives = 210/362 (58.01%), Query Frame = 0

Query: 46  FIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLI 105
           F FGDS  + GNNNY ++    ++NF PYG+T F FPTGR  DGRI+ DF+AEYA LPLI
Sbjct: 39  FAFGDSLFEAGNNNYFDSISSFRSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLI 98

Query: 106 EAYLDP--KNKNYMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVL 165
              L P   N     G+NF +   G  A T           FP             S+  
Sbjct: 99  PPNLQPGYSNSQLTYGLNFATTAAGVFAGT-----------FP------------GSVTN 158

Query: 166 QAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTI 225
            +  L TQ+  FKNVEK+LR  LG+A ++  +S +V+LF+ G NDYQ PF  +   F   
Sbjct: 159 LSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTSTFSN- 218

Query: 226 EAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQF---FQETSS 285
             +++F++ V+GN T  ++ELYK G RKFGF+++ P G  PSA +  S +    F+  + 
Sbjct: 219 TTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSCFEPVTE 278

Query: 286 LSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFR 345
           L  +HN+E    ++RL  +L GFK+A  D HT L QRI NP++YGFK  + ACCGS   R
Sbjct: 279 LINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLR 338

Query: 346 GYYNCGRKKG-TVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQL 402
           G   CG + G +  Y  C+N  DY+F+D  H TE A +Q+A+L+WSG   V  PYN K L
Sbjct: 339 GINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTL 376

BLAST of Clc02G01160 vs. ExPASy TrEMBL
Match: A0A6J1EJ18 (GDSL esterase/lipase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434897 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.9e-165
Identity = 290/408 (71.08%), Postives = 325/408 (79.66%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNY 60
           MKISK+G  FFFF   +L G  AA SGFP I++ D+  SE   GFFIFGDSYVD GNNNY
Sbjct: 1   MKISKMGSFFFFF--FILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNY 60

Query: 61  INTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYMNGV 120
           INTT D  ANFPPYGETFF  PTGRF DGRIIPDFLAEYA LPLI+ YLDPKN  Y+NGV
Sbjct: 61  INTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGV 120

Query: 121 NFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEK 180
           NF SGGGGALA +HQ                            QAIGLQTQMKFFK V+ 
Sbjct: 121 NFASGGGGALAGSHQG---------------------------QAIGLQTQMKFFKRVKN 180

Query: 181 SLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIA 240
           SL K+LGNAR+QSF SNSVFLFNFGGNDY NPFDISYDIFKT++AQ+QF ++V+GNITIA
Sbjct: 181 SLTKRLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIA 240

Query: 241 LKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLATQL 300
           LKE+YK GGRKFGFMTVPPLGY PS+RLK++ QFF ETSSL+R+HNK   +AIQRLATQL
Sbjct: 241 LKEVYKCGGRKFGFMTVPPLGYTPSSRLKKNAQFFDETSSLARVHNKLFLMAIQRLATQL 300

Query: 301 KGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNI 360
            GFK+AFADTHTLLLQRI NPTKYGFK+ DTACCGSD+FRG++NCGRKKG +PYT C+N+
Sbjct: 301 DGFKYAFADTHTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKGAIPYTLCENV 360

Query: 361 QDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTLAS 409
           QDY+F+DSYHPTEK FEQLAK MWSG   +VKPYNFKQLFQYD  L S
Sbjct: 361 QDYMFFDSYHPTEKTFEQLAKEMWSGDAHIVKPYNFKQLFQYDPFLGS 379

BLAST of Clc02G01160 vs. ExPASy TrEMBL
Match: A0A6J1I3Y7 (GDSL esterase/lipase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111470363 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.2e-164
Identity = 288/406 (70.94%), Postives = 326/406 (80.30%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNY 60
           MKISK+G  FFFF   +L GN AA SGFP I++PD+  SE   GFFIFGDSYVD GNNNY
Sbjct: 1   MKISKMGSFFFFF---ILLGNAAAGSGFPGIQLPDIPSSENRSGFFIFGDSYVDAGNNNY 60

Query: 61  INTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYMNGV 120
           INTT D  AN+PPYGETFF  PTGRF DGRIIPDFLAEYA+LPLI+ YLDPKN  Y+NGV
Sbjct: 61  INTTGDFLANYPPYGETFFTTPTGRFSDGRIIPDFLAEYAKLPLIQPYLDPKNNLYLNGV 120

Query: 121 NFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEK 180
           NF SGGGGALAE+HQ                            QAIGLQTQMKFFK V+ 
Sbjct: 121 NFASGGGGALAESHQG---------------------------QAIGLQTQMKFFKRVKN 180

Query: 181 SLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIA 240
           SL K+LG+AR++SF SNSVFLFNFGGNDY NPFDISYDIFKT++AQ+QF N+V+GNITIA
Sbjct: 181 SLTKRLGSARTRSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFANMVIGNITIA 240

Query: 241 LKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLATQL 300
           LKE+YK GGRKFGFMTVPP GY PS+RLK+S QFF ETSSL+R+HNK   +AIQRLATQL
Sbjct: 241 LKEVYKCGGRKFGFMTVPPFGYTPSSRLKKSAQFFYETSSLARVHNKLFLMAIQRLATQL 300

Query: 301 KGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNI 360
            GFK+AFADTHTLLL+RI NPTKYGFK+ DTACCGSD+FRG++NCGRKKG +PYT C+NI
Sbjct: 301 HGFKYAFADTHTLLLRRILNPTKYGFKIIDTACCGSDEFRGFFNCGRKKGALPYTLCENI 360

Query: 361 QDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTL 407
           QDY+F+DSYHPTEK FEQLA  MWSG  ++VK YNFKQLFQYD  L
Sbjct: 361 QDYMFFDSYHPTEKTFEQLANEMWSGDADIVKSYNFKQLFQYDPFL 376

BLAST of Clc02G01160 vs. ExPASy TrEMBL
Match: A0A6J1EIS0 (GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 8.6e-155
Identity = 280/415 (67.47%), Postives = 321/415 (77.35%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPE------IEMPDVGWSEKGFGFFIFGDSYVD 60
           MK   LG CFFF    +LF   AAQ GFP       I++P    SE  FGFFIFGDSYVD
Sbjct: 1   MKFPNLGSCFFF----ILF---AAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVD 60

Query: 61  GGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNK 120
            GNNNYINTT D QANFPPYGE+FF   TGRF DGR IPDFL EYA LPL+  YLDP N 
Sbjct: 61  VGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHND 120

Query: 121 NYMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKF 180
            Y  GVNF SGGGGALA +HQE                           QA+GLQTQMKF
Sbjct: 121 LYDYGVNFASGGGGALALSHQE---------------------------QALGLQTQMKF 180

Query: 181 FKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVV 240
           FK V+KSLRKKLGNAR+QSF SNSVFLFNFGGNDY NPFDISYDIFKTI+AQ+QFVN+V+
Sbjct: 181 FKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQFVNMVI 240

Query: 241 GNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQ 300
           GNIT ALKE+YK GGRKFG M VPPLGY+PS+RLK+S QFF+E SS++R+HNK L IA+ 
Sbjct: 241 GNITKALKEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQFFEEASSIARLHNKLLPIALH 300

Query: 301 RLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPY 360
           +L+ QLKGFK+AFADTHTLLLQRI NP++YGFKV DTACCGSD+FRG YNCGRK G++P+
Sbjct: 301 KLSKQLKGFKYAFADTHTLLLQRILNPSRYGFKVVDTACCGSDEFRGVYNCGRKLGSLPF 360

Query: 361 THCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYD-STLAS 409
           THCKN++D++F+DS+HPTEK F+QL + MWSGG EVVKPYNFKQLF Y+ +TLAS
Sbjct: 361 THCKNLEDHMFFDSFHPTEKVFKQLTEQMWSGGLEVVKPYNFKQLFDYEPTTLAS 381

BLAST of Clc02G01160 vs. ExPASy TrEMBL
Match: A0A6J1I9A0 (GDSL esterase/lipase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470360 PE=3 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.3e-150
Identity = 271/415 (65.30%), Postives = 311/415 (74.94%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPE------IEMPDVGWSEKGFGFFIFGDSYVD 60
           MK      CFFF    +LF    AQ GFP       I++     SE  FGFFIFGDSYVD
Sbjct: 1   MKFPNRSSCFFF----ILF---VAQCGFPTISEAVGIQLTGAPSSENRFGFFIFGDSYVD 60

Query: 61  GGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNK 120
            GNNNYINTT D QANFPPYGE+FF   TGRF DGR IPDFL EYA LPL+  YLDP N 
Sbjct: 61  AGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHND 120

Query: 121 NYMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKF 180
            Y  GVNF SGGGGAL  +HQE                           QA+GLQTQM F
Sbjct: 121 LYDYGVNFASGGGGALPLSHQE---------------------------QALGLQTQMNF 180

Query: 181 FKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVV 240
           FK V+KSLRKKLGNAR+QSF SNSVFLFNFGGNDY NPFDISYDIFKTI+ Q+QFVN+V+
Sbjct: 181 FKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDTQEQFVNMVI 240

Query: 241 GNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQ 300
           GNIT  LKE+YK GGRKFG M VPPLGY+PS+RLK+S Q+F+E SS++R+HNK L IA+ 
Sbjct: 241 GNITTVLKEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQYFEEASSIARLHNKLLPIALH 300

Query: 301 RLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPY 360
           +L+ QLKGFK+AFAD H LLLQRI NP++YGFKV DTACCGSD+FRG YNCGRK G+ P+
Sbjct: 301 KLSKQLKGFKYAFADVHMLLLQRILNPSQYGFKVVDTACCGSDEFRGVYNCGRKLGSFPF 360

Query: 361 THCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTLASD 410
           THCKNI+D++F+DSYHPTEK F+QLA+ MWSGG EVV PYNFKQLF+Y+ TLAS+
Sbjct: 361 THCKNIEDHMFFDSYHPTEKVFKQLAEQMWSGGLEVVTPYNFKQLFEYEPTLASE 381

BLAST of Clc02G01160 vs. ExPASy TrEMBL
Match: A0A6J1G6L0 (GDSL esterase/lipase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451341 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.9e-149
Identity = 267/411 (64.96%), Postives = 310/411 (75.43%), Query Frame = 0

Query: 1   MKISKLGFCFFFFSIIVLFGNGAAQSGFPE---IEMPDVGWSEKGFGFFIFGDSYVDGGN 60
           MK+  LGFCFFFF   +LF    A   FPE   I +P+       F FF+FGDSYVD GN
Sbjct: 1   MKLPNLGFCFFFF--FILFALHTASPTFPEATTIPLPNC------FAFFLFGDSYVDAGN 60

Query: 61  NNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKNKNYM 120
           NNYINTT D QANFPPYGETFFH PT RF DGR IPDFLAEYA LPLI  YLDP    Y 
Sbjct: 61  NNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYD 120

Query: 121 NGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKN 180
           NGVNF S G GALAE+H+                             AIGLQTQMKFFK 
Sbjct: 121 NGVNFASSGSGALAESHEG---------------------------SAIGLQTQMKFFKK 180

Query: 181 VEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNI 240
           V KSLRKKLGNA +Q+ +SNS+FLFNFGGNDY NPFDISYDIF T  AQ++FVN+VVGNI
Sbjct: 181 VMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNI 240

Query: 241 TIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQFFQETSSLSRIHNKELFIAIQRLA 300
           TIALKE+YK+GGRKFGFMTVPPLG++PS+R+KRS QFF+E SS++R+HN+ L  A+Q+L 
Sbjct: 241 TIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSVQFFEEASSITRLHNQLLPDALQKLG 300

Query: 301 TQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHC 360
            QL+GFK+AFADTHTLLLQRI NP KYGFKV +TACCGS  FRG YNCGR    +P+THC
Sbjct: 301 IQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCGRNVEGLPFTHC 360

Query: 361 KNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQLFQYDSTLAS 409
            N++DY+F+DS+HPTEK F+QLA+L+WSG  ++VKPYNFKQLF+YDS LAS
Sbjct: 361 DNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVKPYNFKQLFEYDSMLAS 376

BLAST of Clc02G01160 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 297.0 bits (759), Expect = 2.4e-80
Identity = 162/402 (40.30%), Postives = 227/402 (56.47%), Query Frame = 0

Query: 8   FCFFFFSIIVLFGNGAAQSGFPEIEMPDVGWSEKGFGFFIFGDSYVDGGNNNYINTTIDS 67
           F FF  S  +LF  G + +                   F+FGDS++D GNNNYINTT   
Sbjct: 18  FIFFIVSSTILFLAGKSSAKISH------NGDNNVTALFLFGDSFLDAGNNNYINTTTLD 77

Query: 68  QANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLIEAYLDPKN-KNYMNGVNFGSGG 127
           QANFPPYG+TFF  PTGRF DGR+I DF+AEYA LPLI  +L+P N +  + GVNF S G
Sbjct: 78  QANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAG 137

Query: 128 GGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVLQAIGLQTQMKFFKNVEKSLRKKL 187
            GAL ET Q  V                           I L+TQ+  +K VE+  R   
Sbjct: 138 AGALVETFQGSV---------------------------INLRTQLDHYKKVERLWRTNF 197

Query: 188 GNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTIEAQQQFVNLVVGNITIALKELYK 247
           G   S+  +S +V+L + G NDY + F  +  +     +  Q V++V+GN+T  + E+YK
Sbjct: 198 GKEESKKRISRAVYLISIGSNDYSSIFLTNQSL---PISMSQHVDIVIGNLTTFIHEIYK 257

Query: 248 FGGRKFGFMTVPPLGYLPSARL---KRSEQFFQETSSLSRIHNKELFIAIQRLATQLKGF 307
            GGRKFGF+ VP LG  P+ R+   K  +   ++ S L+ +HN+ L   + ++  Q+KGF
Sbjct: 258 IGGRKFGFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGF 317

Query: 308 KFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFRGYYNCGRKKGTVPYTHCKNIQDY 367
           KF+  D +  L  R+Q+P+K+GFK  + ACCG+  +RG ++CG K+    Y  C+N +DY
Sbjct: 318 KFSLFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDY 377

Query: 368 LFYDSYHPTEKAFEQLAKLMWSGG----DEVVKPYNFKQLFQ 402
           +F+DS H T+  + Q A L+W+GG      VV PYN   LFQ
Sbjct: 378 IFWDSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383

BLAST of Clc02G01160 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 294.7 bits (753), Expect = 1.2e-79
Identity = 158/362 (43.65%), Postives = 216/362 (59.67%), Query Frame = 0

Query: 46  FIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLI 105
           F+FGDS  D GNNNYI+T    ++N+ PYG+T F  PTGR  DGR+IPDF+AEYA LPLI
Sbjct: 39  FVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLI 98

Query: 106 EAYLDPKNKN--YMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVL 165
              L P N N  +  GVNF SGG GAL  T               FS   +N        
Sbjct: 99  PPNLQPFNGNSQFAYGVNFASGGAGALVGT---------------FSGLVIN-------- 158

Query: 166 QAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTI 225
               L+TQ+  FK VE+ LR KLG+A  +  +S +V+LF+ G NDYQ PF  +  +F++I
Sbjct: 159 ----LRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI 218

Query: 226 EAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARL---KRSEQFFQETSS 285
            + +++V+ VVGN+T   KE+Y  GGRKFG +   P    P++ +    +    FQ  + 
Sbjct: 219 -SNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTE 278

Query: 286 LSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFR 345
           L  +HN++L   ++RL  +L GFK+A  D HT L +R+ +P+KYGFK    ACCGS   R
Sbjct: 279 LINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLR 338

Query: 346 GYYNCGRKKG-TVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQL 402
           G   CG + G +  Y  C+N+ DYLF+D +H TEKA  Q+A+L+WSG   +  PYN K L
Sbjct: 339 GINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKAL 372

BLAST of Clc02G01160 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 282.3 bits (721), Expect = 6.0e-76
Identity = 154/362 (42.54%), Postives = 210/362 (58.01%), Query Frame = 0

Query: 46  FIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLI 105
           F FGDS  + GNNNY ++    ++NF PYG+T F FPTGR  DGRI+ DF+AEYA LPLI
Sbjct: 39  FAFGDSLFEAGNNNYFDSISSFRSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLI 98

Query: 106 EAYLDP--KNKNYMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVL 165
              L P   N     G+NF +   G  A T           FP             S+  
Sbjct: 99  PPNLQPGYSNSQLTYGLNFATTAAGVFAGT-----------FP------------GSVTN 158

Query: 166 QAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTI 225
            +  L TQ+  FKNVEK+LR  LG+A ++  +S +V+LF+ G NDYQ PF  +   F   
Sbjct: 159 LSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTSTFSN- 218

Query: 226 EAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQF---FQETSS 285
             +++F++ V+GN T  ++ELYK G RKFGF+++ P G  PSA +  S +    F+  + 
Sbjct: 219 TTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSCFEPVTE 278

Query: 286 LSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFR 345
           L  +HN+E    ++RL  +L GFK+A  D HT L QRI NP++YGFK  + ACCGS   R
Sbjct: 279 LINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLR 338

Query: 346 GYYNCGRKKG-TVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQL 402
           G   CG + G +  Y  C+N  DY+F+D  H TE A +Q+A+L+WSG   V  PYN K L
Sbjct: 339 GINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTL 376

BLAST of Clc02G01160 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 280.4 bits (716), Expect = 2.3e-75
Identity = 150/347 (43.23%), Postives = 204/347 (58.79%), Query Frame = 0

Query: 46  FIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLI 105
           F+FGDS  D GNNNYI+T    ++N+ PYG+T F FPTGR  DGR IPDF+AEYA LPLI
Sbjct: 41  FVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLI 100

Query: 106 EAYLDPKN--KNYMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVL 165
            AYL P N    +  GV+F S G GAL  T    V                         
Sbjct: 101 PAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMV------------------------- 160

Query: 166 QAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTI 225
             I L++Q+  FK VEK LR  LG A+ +  +S +V+LF+ G NDYQ PF  +  IF++ 
Sbjct: 161 --INLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS- 220

Query: 226 EAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQF---FQETSS 285
             Q+ +V+ VVGN T  +KE+YK GGRKFGF+ +      P++ +    +    F+  + 
Sbjct: 221 SPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTE 280

Query: 286 LSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFR 345
           L  +HN++L   ++RL  +L GFK+A  D HT L  R+ NP+KYGFK    ACCG+   R
Sbjct: 281 LINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLR 340

Query: 346 GYYNCGRKKG-TVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSG 387
           G   CG + G +  Y  C+ + DYLF+D +H TEKA +Q+A+L+WSG
Sbjct: 341 GINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSG 359

BLAST of Clc02G01160 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 278.9 bits (712), Expect = 6.7e-75
Identity = 155/362 (42.82%), Postives = 209/362 (57.73%), Query Frame = 0

Query: 46  FIFGDSYVDGGNNNYINTTIDSQANFPPYGETFFHFPTGRFCDGRIIPDFLAEYAELPLI 105
           F+FGDS  D GNNNYINT    ++N  PYG+T F FPTGR  DG        E A LP I
Sbjct: 37  FVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPSI 96

Query: 106 EAYLDPKNKN--YMNGVNFGSGGGGALAETHQEQVPINLISFPVFFSSAELNAYNFSLVL 165
              L P N N  +  GV+F S G GALAE                           S + 
Sbjct: 97  PPNLQPNNGNNQFTYGVSFASAGAGALAE---------------------------SFLG 156

Query: 166 QAIGLQTQMKFFKNVEKSLRKKLGNARSQSFLSNSVFLFNFGGNDYQNPFDISYDIFKTI 225
             I L TQ+  FK+VEKSLR +LG+A ++   S +V+LF+ G NDY  PF  +   FK+ 
Sbjct: 157 MVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFKS- 216

Query: 226 EAQQQFVNLVVGNITIALKELYKFGGRKFGFMTVPPLGYLPSARLKRSEQF---FQETSS 285
            ++++FV+ V+GNIT  ++E+YK GGRKFGF+ V P    P++ ++   +    F+  + 
Sbjct: 217 NSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAE 276

Query: 286 LSRIHNKELFIAIQRLATQLKGFKFAFADTHTLLLQRIQNPTKYGFKVADTACCGSDDFR 345
           L  +HNK+    ++RL  QL GF++A  D HT L +RI +P+KYGFK    ACCGS   R
Sbjct: 277 LIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLR 336

Query: 346 GYYNCGRKKG-TVPYTHCKNIQDYLFYDSYHPTEKAFEQLAKLMWSGGDEVVKPYNFKQL 402
           G   CG + G +  Y  C+N+ DYLFYDS H TEKA  Q+A+L+W+G   V +PYN K L
Sbjct: 337 GINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKAL 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022927996.13.8e-16571.08GDSL esterase/lipase 1-like [Cucurbita moschata][more]
XP_038901400.11.9e-16469.68GDSL lipase-like isoform X3 [Benincasa hispida][more]
XP_022971691.12.5e-16470.94GDSL esterase/lipase 1-like [Cucurbita maxima][more]
XP_023512753.13.0e-16270.18GDSL esterase/lipase 1-like [Cucurbita pepo subsp. pepo][more]
XP_038901398.14.8e-16065.37GDSL lipase-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
H6U1I83.9e-8042.58GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1[more]
Q9SSA73.3e-7940.30GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2[more]
Q9SYF09.7e-7943.37GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1[more]
Q9FLN01.7e-7843.65GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1[more]
Q9LJP18.5e-7542.54GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1EJ181.9e-16571.08GDSL esterase/lipase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434897 PE=3 S... [more]
A0A6J1I3Y71.2e-16470.94GDSL esterase/lipase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111470363 PE=3 SV=... [more]
A0A6J1EIS08.6e-15567.47GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 S... [more]
A0A6J1I9A01.3e-15065.30GDSL esterase/lipase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470360 PE=3 SV=... [more]
A0A6J1G6L01.9e-14964.96GDSL esterase/lipase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
Match NameE-valueIdentityDescription
AT1G53920.12.4e-8040.30GDSL-motif lipase 5 [more]
AT5G40990.11.2e-7943.65GDSL lipase 1 [more]
AT3G14225.16.0e-7642.54GDSL-motif lipase 4 [more]
AT1G53940.12.3e-7543.23GDSL-motif lipase 2 [more]
AT1G53990.16.7e-7542.82GDSL-motif lipase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 46..382
e-value: 4.1E-26
score: 92.8
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 44..387
e-value: 1.8E-67
score: 230.1
IPR044552GDSL esterase/lipase GLIP1-5/GLL25PANTHERPTHR45966GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 147..401
IPR044552GDSL esterase/lipase GLIP1-5/GLL25PANTHERPTHR45966GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 10..138
NoneNo IPR availablePANTHERPTHR45966:SF4GDSL ESTERASE/LIPASE 5coord: 10..138
coord: 147..401
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 45..385
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 45..386
e-value: 1.62153E-100
score: 299.916

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc02G01160.1Clc02G01160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds