Clc01G22270 (gene) Watermelon (cordophanus) v2

Overview
NameClc01G22270
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Descriptionmitogen-activated protein kinase kinase kinase 2-like
LocationClcChr01: 33514820 .. 33516238 (+)
RNA-Seq ExpressionClc01G22270
SyntenyClc01G22270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTGGATGAGAGGCCGGACCATCGGCCGTGGCTCCTCCGCTGCGGTCTCCATCGCCACCGATCTTCGGTCTGGTCAAAGGCTGGCGGTTAAGACAGTTGAGTTCACTCACTTGGATTTTCTCGAGAGGGAACACAGGATTCTTTCTCAATTGAATTGTTCTAATGTTATAGGTTATAAGGGGTTTGATGTTACGTTCGAAAATGGGCATTTGATGTGCAATCTTTTGATGGAGTTTGCGCCTGGTGGGTCGATTTTGGATGCGATGGAGGTGGCGGGTGGCCGATTAGATGAAGCGACAGTCCAATTATATACTCGAGAGATTTTAAGTGGGCTCGAGTATGTTCATTCTAATGGTGTGGTACATTGTGATATTAAATGTTGTAATATTTTGGTTGGTGAAGATGGGATCAAGATTGCGGATTTTGGTTCCGCGCGGCGGGTGGAGGAAGTTTCCTGTGGAAATTTGGCTGGAACGCCGATATTCATGGCGCCAGAGGTGGCGCGTGGAGAAAAACAGGGGTTTGCAGCTGATGTTTGGGGAGTGGGTTGCGCAGTGATCCAGATGGTGACAGGCCGAGCTCCGTGGGCGAATGTGTCGGATCCGGTGGCCGCCATTTACAGAATTGGGTCCGGCGATGATTTGCCGGAGATTCCTGGGTTCATGTCAGAACAGGGCAAGGACTTTTTGCGGCGGTGTTTGATCAGAGACCCAGAAGAACGGTGGTCTGTGAATGAGCTTTTGAAACACCCTTTTGTTCACGAGCAGAGATCCCAACCTAAACAGAATTCAAGAACACCGACGAGTATTTTGGAACAAGCATTATGGGACACGATGAACGATTCGGAAACGGTTCAAACTCCGGTTCAAAGTCCGATCAGACCAAAAATTCACAGAACGCCATTGCAGAGGATACAGCAACTAAGCGAAATTTCCACCAATCAGTCTTCTGGAATAGCCAATTGGGAGTGCGGTGAAGATTGGATAACAGTGAGAAGCATTGGGTTGGAAGAGGAAAACAGAGAAGAAAACGATTTCGTTTCTGTACTGGAAATGCCCTGTTCTTCTCAATCCATCGATATGGCAACTAACAATGGAGTTGGTGATCAGGATTATATTAATGTTAGTGTTAGTAGAAGTAGTGATTATTATAGCAGAAAAAGCAGTAAGGCTAAGAACAGAGACTTGCCTGCTATGTCCTCTGCTTGCAACCGAATTGTATTATTGAAGACTGTTTGTTTTTCTAATAATTATAAATTATGTATTATCATTTCTTTGTTTCTTGTCTTTCCAGTCAGGGTTTTATTTCATCTTACATTTTTGAGATATAATGAAGATATTTTGATTTATAACGAAAATATGAACATGTCGATGGATCTCTGTTTACTTGAGTTGAGTATCTTGTTTGAAGACTGA

mRNA sequence

ATGGAGTGGATGAGAGGCCGGACCATCGGCCGTGGCTCCTCCGCTGCGGTCTCCATCGCCACCGATCTTCGGTCTGGTCAAAGGCTGGCGGTTAAGACAGTTGAGTTCACTCACTTGGATTTTCTCGAGAGGGAACACAGGATTCTTTCTCAATTGAATTGTTCTAATGTTATAGGTTATAAGGGGTTTGATGTTACGTTCGAAAATGGGCATTTGATGTGCAATCTTTTGATGGAGTTTGCGCCTGGTGGGTCGATTTTGGATGCGATGGAGGTGGCGGGTGGCCGATTAGATGAAGCGACAGTCCAATTATATACTCGAGAGATTTTAAGTGGGCTCGAGTATGTTCATTCTAATGGTGTGGTACATTGTGATATTAAATGTTGTAATATTTTGGTTGGTGAAGATGGGATCAAGATTGCGGATTTTGGTTCCGCGCGGCGGGTGGAGGAAGTTTCCTGTGGAAATTTGGCTGGAACGCCGATATTCATGGCGCCAGAGGTGGCGCGTGGAGAAAAACAGGGGTTTGCAGCTGATGTTTGGGGAGTGGGTTGCGCAGTGATCCAGATGGTGACAGGCCGAGCTCCGTGGGCGAATGTGTCGGATCCGGTGGCCGCCATTTACAGAATTGGGTCCGGCGATGATTTGCCGGAGATTCCTGGGTTCATGTCAGAACAGGGCAAGGACTTTTTGCGGCGGTGTTTGATCAGAGACCCAGAAGAACGGTGGTCTGTGAATGAGCTTTTGAAACACCCTTTTGTTCACGAGCAGAGATCCCAACCTAAACAGAATTCAAGAACACCGACGAGTATTTTGGAACAAGCATTATGGGACACGATGAACGATTCGGAAACGGTTCAAACTCCGGTTCAAAGTCCGATCAGACCAAAAATTCACAGAACGCCATTGCAGAGGATACAGCAACTAAGCGAAATTTCCACCAATCAGTCTTCTGGAATAGCCAATTGGGAGTGCGGTGAAGATTGGATAACAGTGAGAAGCATTGGGTTGGAAGAGGAAAACAGAGAAGAAAACGATTTCGTTTCTGTACTGGAAATGCCCTGTTCTTCTCAATCCATCGATATGGCAACTAACAATGGAGTTGGTGATCAGGATTATATTAATGTTAGTGTTAGTAGAAGTAGTGATTATTATAGCAGAAAAAGCAGTAAGGCTAAGAACAGAGACTTGCCTGCTATGTCCTCTGCTTGCAACCGAATTGTATTATTGAAGACTGTTTGTTTTTCTAATAATTATAAATTATGTATTATCATTTCTTTGTTTCTTGTCTTTCCAGTCAGGGTTTTATTTCATCTTACATTTTTGAGATATAATGAAGATATTTTGATTTATAACGAAAATATGAACATGTCGATGGATCTCTGTTTACTTGAGTTGAGTATCTTGTTTGAAGACTGA

Coding sequence (CDS)

ATGGAGTGGATGAGAGGCCGGACCATCGGCCGTGGCTCCTCCGCTGCGGTCTCCATCGCCACCGATCTTCGGTCTGGTCAAAGGCTGGCGGTTAAGACAGTTGAGTTCACTCACTTGGATTTTCTCGAGAGGGAACACAGGATTCTTTCTCAATTGAATTGTTCTAATGTTATAGGTTATAAGGGGTTTGATGTTACGTTCGAAAATGGGCATTTGATGTGCAATCTTTTGATGGAGTTTGCGCCTGGTGGGTCGATTTTGGATGCGATGGAGGTGGCGGGTGGCCGATTAGATGAAGCGACAGTCCAATTATATACTCGAGAGATTTTAAGTGGGCTCGAGTATGTTCATTCTAATGGTGTGGTACATTGTGATATTAAATGTTGTAATATTTTGGTTGGTGAAGATGGGATCAAGATTGCGGATTTTGGTTCCGCGCGGCGGGTGGAGGAAGTTTCCTGTGGAAATTTGGCTGGAACGCCGATATTCATGGCGCCAGAGGTGGCGCGTGGAGAAAAACAGGGGTTTGCAGCTGATGTTTGGGGAGTGGGTTGCGCAGTGATCCAGATGGTGACAGGCCGAGCTCCGTGGGCGAATGTGTCGGATCCGGTGGCCGCCATTTACAGAATTGGGTCCGGCGATGATTTGCCGGAGATTCCTGGGTTCATGTCAGAACAGGGCAAGGACTTTTTGCGGCGGTGTTTGATCAGAGACCCAGAAGAACGGTGGTCTGTGAATGAGCTTTTGAAACACCCTTTTGTTCACGAGCAGAGATCCCAACCTAAACAGAATTCAAGAACACCGACGAGTATTTTGGAACAAGCATTATGGGACACGATGAACGATTCGGAAACGGTTCAAACTCCGGTTCAAAGTCCGATCAGACCAAAAATTCACAGAACGCCATTGCAGAGGATACAGCAACTAAGCGAAATTTCCACCAATCAGTCTTCTGGAATAGCCAATTGGGAGTGCGGTGAAGATTGGATAACAGTGAGAAGCATTGGGTTGGAAGAGGAAAACAGAGAAGAAAACGATTTCGTTTCTGTACTGGAAATGCCCTGTTCTTCTCAATCCATCGATATGGCAACTAACAATGGAGTTGGTGATCAGGATTATATTAATGTTAGTGTTAGTAGAAGTAGTGATTATTATAGCAGAAAAAGCAGTAAGGCTAAGAACAGAGACTTGCCTGCTATGTCCTCTGCTTGCAACCGAATTGTATTATTGAAGACTGTTTGTTTTTCTAATAATTATAAATTATGTATTATCATTTCTTTGTTTCTTGTCTTTCCAGTCAGGGTTTTATTTCATCTTACATTTTTGAGATATAATGAAGATATTTTGATTTATAACGAAAATATGAACATGTCGATGGATCTCTGTTTACTTGAGTTGAGTATCTTGTTTGAAGACTGA

Protein sequence

MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGYKGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNGVVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADVWGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPEERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHRTPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSIDMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRIVLLKTVCFSNNYKLCIIISLFLVFPVRVLFHLTFLRYNEDILIYNENMNMSMDLCLLELSILFED
Homology
BLAST of Clc01G22270 vs. NCBI nr
Match: XP_038883085.1 (mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida])

HSP 1 Score: 797.3 bits (2058), Expect = 6.9e-227
Identity = 411/505 (81.39%), Postives = 435/505 (86.14%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEWMRGRTIGRGSSAAVS+ATDLRSGQRLAVK+VEFTHLDFL+RE RILSQLNCSNVIGY
Sbjct: 1   MEWMRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHLDFLKREQRILSQLNCSNVIGY 60

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT E+G+LMCNLLMEFA GGSILDAME AGGRLDEAT QLYTREILSGLEYVHSNG
Sbjct: 61  KGFDVTLEDGNLMCNLLMEFASGGSILDAMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFG ARRVEEVS GN AGTPIFMAPEVARG+KQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEEVSGGNWAGTPIFMAPEVARGKKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           WGVGCAVIQMVTGR PWANVSDPVAAIYRIG+GD+LPEIP FMSEQGKDFLRRCLIRDPE
Sbjct: 181 WGVGCAVIQMVTGRPPWANVSDPVAAIYRIGAGDELPEIPRFMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTP----------- 300
           +RWSVNELLKHPFV+EQ+S PKQNSRTP SILEQ LWDTMNDSET+++P           
Sbjct: 241 KRWSVNELLKHPFVYEQKSHPKQNSRTPRSILEQGLWDTMNDSETIRSPVQSPIRRKIHR 300

Query: 301 ----------------------VQSPIRPKIHRTPLQRIQQLSEISTNQSSGIANWECGE 360
                                 VQSPIRPKIHRTPLQRIQQLSEIST QSSG  NWEC E
Sbjct: 301 TPSQRISILEQGLWDTMNDSETVQSPIRPKIHRTPLQRIQQLSEISTIQSSGTPNWECDE 360

Query: 361 DWITVRSIGLEEENREENDFVSVLEMPCSSQSIDMATNNGVGDQDYINVSVSRSSDYYSR 420
           DW+TVRSIGLEEE+REEN+FVSVLEMPCSSQSI+ A NNG  DQDYINVS S+ SDY SR
Sbjct: 361 DWVTVRSIGLEEEDREENNFVSVLEMPCSSQSIERAINNGAVDQDYINVSDSKISDYCSR 420

Query: 421 KSSKAKNRDLPAMSSACNRIVLLKTVCFSNNYKLCIIISLFLVFPVRVLFHLTFLRYNED 473
           K SK KNRDLPAMSS CN+IV LKT   SNN+KLCIIISLFLVFP R    LTFLRYNED
Sbjct: 421 KISKVKNRDLPAMSSVCNQIVSLKTT-LSNNHKLCIIISLFLVFPPRGSLDLTFLRYNED 480

BLAST of Clc01G22270 vs. NCBI nr
Match: XP_008441103.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 759.2 bits (1959), Expect = 2.1e-215
Identity = 388/474 (81.86%), Postives = 417/474 (87.97%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW+RGRT+GRGSSAAVS+ATDLRSGQ +AVKTVEF+ LDFL+RE RILSQLNCS VIGY
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG+LMCNLLMEFAPGGSILD ME AGGRLDEAT Q YTREILSGL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFG ARRVE+V  GNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGCAVIQMVTGRAPWAN+SDP+AAIYRIGSGDDLPEIP  MSEQGKDFLRRCLIRDPE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSVNELLKHPFV EQ+S PKQNSRTPTSILEQ +WDT+ND ET    V+SPIRPKIHR
Sbjct: 241 ERWSVNELLKHPFVQEQKSNPKQNSRTPTSILEQGIWDTVNDPET----VESPIRPKIHR 300

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSI 360
           TPLQRIQQL+E+STN      NWEC EDWITVRSIGL     EEND VSV+E PCSSQS+
Sbjct: 301 TPLQRIQQLNEVSTNG----PNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSV 360

Query: 361 DMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRIVLLKTVCFSNNYK 420
           +MA+NN VGDQDYINVS SRSSDY SRKSSKAKNRD PAMSS  N+IVLLKT+ FSNN+K
Sbjct: 361 EMASNNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQIVLLKTINFSNNHK 420

Query: 421 LCIIISLFLVFPVRVLFHLTFLRYNEDILIYNENMNMSMDLCLLEL--SILFED 473
           LCIIISLFLVFP R LF+L  L YNE+  IY+EN  MS+DLCLLEL  ++LF D
Sbjct: 421 LCIIISLFLVFPARGLFYLIILSYNENTSIYHENTKMSIDLCLLELNMNVLFND 461

BLAST of Clc01G22270 vs. NCBI nr
Match: XP_023544013.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 704.5 bits (1817), Expect = 6.1e-199
Identity = 364/473 (76.96%), Postives = 399/473 (84.36%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           M+W RG TIGRGSSAAVS+ATDL SGQ LAVK+VEFTH DFL+RE RILS+ NC  VI Y
Sbjct: 20  MDWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTHSDFLKREQRILSRFNCPRVIAY 79

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG LMCNLLMEFAPGGSILD ME AGGRLDEAT Q YTREILSGLEYVHSNG
Sbjct: 80  KGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEATAQFYTREILSGLEYVHSNG 139

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFG AR+VEE S  NL GTP+FMAPEVARGEKQGFAADV
Sbjct: 140 VVHCDIKCCNILVGEDGIKIADFGCARQVEEDSSRNLVGTPMFMAPEVARGEKQGFAADV 199

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGCAVIQMVTGRAPW NVSDPVAAIY+IGSGDDLPE+P FMSEQGKDFLRRCLIRDP 
Sbjct: 200 WAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPRFMSEQGKDFLRRCLIRDPG 259

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSV+ELLKHPFVHEQ+S PKQNSRTPTSILEQ  W TMN+SET    VQSPI PK++R
Sbjct: 260 ERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMNNSET----VQSPIMPKLYR 319

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSI 360
           TPLQRIQQLSEIS  QSS I NW+C +DW+TV+SI +EEE+ E+N+FVSVLE+PC SQSI
Sbjct: 320 TPLQRIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQNEFVSVLELPCFSQSI 379

Query: 361 DMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRIVLLKTVCFSNNYK 420
            MAT NGVGDQ+YI+VS+       SR+SSKAK RDLPAMSS  N+IV L T  FSNN+K
Sbjct: 380 KMATKNGVGDQNYIDVSI-------SRESSKAKKRDLPAMSSVSNQIVSLNTNSFSNNHK 439

Query: 421 LCIIISLFLVFPVR-VLFHLTFLRYNEDILIYNENMNMSMDLCLLELSILFED 473
           LCII SLFLVF        +TFLRY E+I IY+EN+ M M+LCL EL+IL  D
Sbjct: 440 LCIISSLFLVFLASGGSIRVTFLRYIENIWIYHENVKMLMNLCLFELNILSGD 481

BLAST of Clc01G22270 vs. NCBI nr
Match: KAG6603788.1 (Mitogen-activated protein kinase kinase kinase 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 698.0 bits (1800), Expect = 5.7e-197
Identity = 361/473 (76.32%), Postives = 397/473 (83.93%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW RG TIGRGSSAAVS+ATDL SGQ LAVK+VEFT  D L+RE RILS+ NC  VI Y
Sbjct: 8   MEWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTRSDCLKREQRILSRFNCPRVIAY 67

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG LMCNLLMEFAPGGSILD ME AGGRLDEAT Q YTREILSGLEYVHSNG
Sbjct: 68  KGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEATAQFYTREILSGLEYVHSNG 127

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFG AR+V +VSC NLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 128 VVHCDIKCCNILVGEDGIKIADFGCARQVGDVSCRNLAGTPIFMAPEVARGEKQGFAADV 187

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGCAVIQMVTGRAPW NVSDPVAAIY+IGSGDDLPE+P F+S+QGKDFLRRCLIRDPE
Sbjct: 188 WAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPRFISDQGKDFLRRCLIRDPE 247

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSV+ELLKHPFVHEQ+S PKQNS TPTSILEQ  W TMN+SETVQ     PI PK++R
Sbjct: 248 ERWSVSELLKHPFVHEQKSPPKQNSTTPTSILEQESWYTMNNSETVQI----PIMPKLYR 307

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSI 360
           TPLQRIQQLSEIS  QSS I NW+C +DW+TV+SI +EEE+ E+++FVSVLE+PC SQSI
Sbjct: 308 TPLQRIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQSEFVSVLELPCFSQSI 367

Query: 361 DMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRIVLLKTVCFSNNYK 420
            MAT NGVGDQ+YINVS+       SR+SSKAK R LPAMSS  N+IV L T  FSNN+K
Sbjct: 368 KMATKNGVGDQNYINVSI-------SRESSKAKKRHLPAMSSVSNQIVSLNTNSFSNNHK 427

Query: 421 LCIIISLFLVFPVR-VLFHLTFLRYNEDILIYNENMNMSMDLCLLELSILFED 473
           LCII SLFLVF        +TFLRY E+I IY+EN+ M M+LCL EL+IL  D
Sbjct: 428 LCIISSLFLVFLASGGSIRVTFLRYIENIWIYHENVKMLMNLCLFELNILSGD 469

BLAST of Clc01G22270 vs. NCBI nr
Match: XP_022950078.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata])

HSP 1 Score: 697.6 bits (1799), Expect = 7.5e-197
Identity = 361/473 (76.32%), Postives = 397/473 (83.93%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW RG TIGRGSSAAVS+ATDL SGQ LAVK+VEFT  D L+RE RILS+ NC  VI Y
Sbjct: 20  MEWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTRSDCLKREQRILSRFNCPRVIAY 79

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG LMCNLLMEFAPGGSILD ME AGGRLDEA  Q YTREILSGLEYVH+NG
Sbjct: 80  KGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEARAQFYTREILSGLEYVHTNG 139

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFG AR+VE+VSC NLAGT IFMAPEVARGEKQGFAADV
Sbjct: 140 VVHCDIKCCNILVGEDGIKIADFGCARQVEDVSCRNLAGTLIFMAPEVARGEKQGFAADV 199

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGCAVIQMVTGRAPW NVSDPVAAIY+IGSGDDLPE+P F+S+QGKDFLRRCLIRDPE
Sbjct: 200 WAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPRFISDQGKDFLRRCLIRDPE 259

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSV+ELLKHPFVHEQ+S PKQNSRTPTSILEQ  W TMN+SETVQ     PI PK++R
Sbjct: 260 ERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMNNSETVQI----PIMPKLYR 319

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSI 360
           TPLQRIQQLSEIS  QSS I NW+C +DW+TV+SI +EEE+ E+++FVSVLE+PC SQSI
Sbjct: 320 TPLQRIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQSEFVSVLELPCFSQSI 379

Query: 361 DMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRIVLLKTVCFSNNYK 420
            MAT NGVGDQ YINVS+       SR+SSKAK RDLPAMSS  N+IV L T  FSNN+K
Sbjct: 380 KMATKNGVGDQTYINVSI-------SRESSKAKKRDLPAMSSVSNQIVSLNTNSFSNNHK 439

Query: 421 LCIIISLFLVFPVR-VLFHLTFLRYNEDILIYNENMNMSMDLCLLELSILFED 473
           LCII SLFLVF        +TFLRY E+I IY+EN+ M M+LCL EL+IL  D
Sbjct: 440 LCIISSLFLVFLASGGSIRVTFLRYIENIWIYHENVKMLMNLCLFELNILSGD 481

BLAST of Clc01G22270 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.3e-76
Identity = 158/352 (44.89%), Postives = 213/352 (60.51%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW RGR +GRGS+A V  A    S + LAVK+ E    +FL+RE +ILS L+   VIGY
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  KGFDVTFE-NGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSN 120
           +G +   E NG +M NLLME+AP G++ DA    GGR+DE  V  YTR+IL GLEY+HS 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GVVHCDIKCCNILVGEDG-IKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAA 180
           G+VHCD+K  N+++ E G  KIADFG A+RV+ V    + GTP FMAPEVARGEKQG  +
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DVWGVGCAVIQMVTGRAPWANV---SDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCL 240
           D+W VGC +I+MVTG  PW       DPV+ +YR+G   + PE+P  ++E+ KDFL +CL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 IRDPEERWSVNELLKHPFVH-----EQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPV 300
            R+  ERW+  +LL HPF+      E    P   S +PTS+ +Q  W ++ + E  +T  
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEE 300

Query: 301 QSPIRPKIHRTPL-----QRIQQLSEISTNQSSGIANWEC---GEDWITVRS 335
                  + R  L     +RI +L  +      G+    C   G DWI VR+
Sbjct: 301 IQKDSRDLDRLSLWGCYSERIGRLKCV-----GGLDGTRCDMEGGDWIMVRA 347

BLAST of Clc01G22270 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.9e-74
Identity = 160/345 (46.38%), Postives = 214/345 (62.03%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           M W RG+T+GRGS+A VS AT   SG+ LAVK+ EF   +FL+RE +ILS LN   VIGY
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  KGFDVTFENGH-----LMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEY 120
           +G ++T E  H        +LLME+AP G++ D     GG +DEA V  YTR+IL GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 VH-SNGVVHCDIKCCNILVGEDG-IKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEK 180
           +H S G+ HCDIK  N+LVGE+G  KIADFG A+ VE      + GTP FMAPE ARGE+
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGFAADVWGVGCAVIQMVTGRAPW--ANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFL 240
           QG  +D+W VGC VI+MVTG  PW  A+ +DPV+ +YR+G   +LPE+P  ++EQ KDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 RRCLIRDPEERWSVNELLKHPFVHEQRSQPKQN--SRTPTSILEQALWDTMNDSETVQTP 300
            +CL ++  ERW+ ++LL HPF+  +  +      + +PTS+ +Q  W       +V+  
Sbjct: 241 GKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMFW------RSVEEE 300

Query: 301 VQSPIRPKIHRTPLQRIQQLSEISTNQSSGIANWEC-GEDWITVR 334
           V             +RI  LS I        + W+  GEDWITVR
Sbjct: 301 VSEDRSSWWECHEDERIGVLSWIGHVVVE--STWDLDGEDWITVR 337

BLAST of Clc01G22270 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.0e-55
Identity = 133/361 (36.84%), Postives = 191/361 (52.91%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSG----QRLAVKTVEFTHLDFLEREHRILSQL-NCS 60
           MEW+RG TIG G+ + VS AT  R+       +AVK+ +      L  E  +L  L +C 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  NVIGYKGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGR-LDEATVQLYTREILSGLE 120
            +I   G D T ENG  M NLL+E+A  GS+   M+  GG  L E+TV+ +T  +L GL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YVHSNGVVHCDIKCCNILVGEDG-IKIADFGSARRVEEVSCG-----NLAGTPIFMAPEV 180
           ++H+ G  HCDIK  NIL+  DG +KIADFG A RV+           + GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGEKQGFAADVWGVGCAVIQMVTGRAPWA--NVSDPVAAIYRIGSGDDLPEIPGFMSEQ 240
               + G AADVW +GCAV++M +G+  W+    S  ++ + RIG GD+LP+IP  +SE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 GKDFLRRCLIRDPEERWSVNELLKHPFV-------HEQRSQPKQNSR-----TPTSILEQ 300
           GKDFL +C ++DP +RW+   LL H FV       H +    K         +P    E 
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 ALWD--TMNDSETVQTPVQSPIRPKIHRTPLQRIQQLSEISTNQSSGIANWECGEDWITV 334
             WD  T++ + +  +PV+                    + +  S  I +W  G  W+TV
Sbjct: 301 DDWDSFTLDSNPSFDSPVE-------------------RLGSLVSGSIPDWSVGGSWLTV 342

BLAST of Clc01G22270 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.4e-51
Identity = 116/291 (39.86%), Postives = 171/291 (58.76%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFT-----------HLDFLEREHRIL 60
           + W +G+ IGRG+   V +  +L SG+ LAVK V  T           H+  LE E ++L
Sbjct: 66  IRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLL 125

Query: 61  SQLNCSNVIGYKGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREI 120
             L+  N++ Y G   T      + N+L+EF PGGSI   +E  G    E+ V+ YT ++
Sbjct: 126 KNLSHPNIVRYLG---TVREDETL-NILLEFVPGGSISSLLEKFGA-FPESVVRTYTNQL 185

Query: 121 LSGLEYVHSNGVVHCDIKCCNILVGEDG-IKIADFGSARRVEEVS----CGNLAGTPIFM 180
           L GLEY+H++ ++H DIK  NILV   G IK+ADFG++++V E++      ++ GTP +M
Sbjct: 186 LLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWM 245

Query: 181 APEVARGEKQGFAADVWGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMS 240
           APEV       F+AD+W VGC VI+MVTG+APW+     +AAI+ IG+    P IP  +S
Sbjct: 246 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNIS 305

Query: 241 EQGKDFLRRCLIRDPEERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQA 276
               DFL +CL ++P  R + +ELLKHPFV     Q +  S+  TS ++ +
Sbjct: 306 SDANDFLLKCLQQEPNLRPTASELLKHPFV--TGKQKESASKDLTSFMDNS 349

BLAST of Clc01G22270 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 203.0 bits (515), Expect = 7.6e-51
Identity = 115/270 (42.59%), Postives = 162/270 (60.00%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEF-----------THLDFLEREHRIL 60
           + W +G+ IGRG+   V +  +L SG+ LAVK V              H+  LE E ++L
Sbjct: 67  ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 126

Query: 61  SQLNCSNVIGYKGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREI 120
             L+  N++ Y G     E+  L  N+L+EF PGGSI   +E   G   E+ V+ YTR++
Sbjct: 127 KNLSHPNIVRYLG--TVREDDTL--NILLEFVPGGSISSLLE-KFGPFPESVVRTYTRQL 186

Query: 121 LSGLEYVHSNGVVHCDIKCCNILVGEDG-IKIADFGSARRVEEVS----CGNLAGTPIFM 180
           L GLEY+H++ ++H DIK  NILV   G IK+ADFG++++V E++      ++ GTP +M
Sbjct: 187 LLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWM 246

Query: 181 APEVARGEKQGFAADVWGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMS 240
           APEV       F+AD+W VGC VI+MVTG+APW+     VAAI+ IG+    P IP  +S
Sbjct: 247 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLS 306

Query: 241 EQGKDFLRRCLIRDPEERWSVNELLKHPFV 255
              KDFL +CL   P  R + +ELLKHPFV
Sbjct: 307 SDAKDFLLKCLQEVPNLRPTASELLKHPFV 331

BLAST of Clc01G22270 vs. ExPASy TrEMBL
Match: A0A1S3B3E3 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485329 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.0e-215
Identity = 388/474 (81.86%), Postives = 417/474 (87.97%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW+RGRT+GRGSSAAVS+ATDLRSGQ +AVKTVEF+ LDFL+RE RILSQLNCS VIGY
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG+LMCNLLMEFAPGGSILD ME AGGRLDEAT Q YTREILSGL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFG ARRVE+V  GNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGCAVIQMVTGRAPWAN+SDP+AAIYRIGSGDDLPEIP  MSEQGKDFLRRCLIRDPE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSVNELLKHPFV EQ+S PKQNSRTPTSILEQ +WDT+ND ET    V+SPIRPKIHR
Sbjct: 241 ERWSVNELLKHPFVQEQKSNPKQNSRTPTSILEQGIWDTVNDPET----VESPIRPKIHR 300

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSI 360
           TPLQRIQQL+E+STN      NWEC EDWITVRSIGL     EEND VSV+E PCSSQS+
Sbjct: 301 TPLQRIQQLNEVSTNG----PNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSV 360

Query: 361 DMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRIVLLKTVCFSNNYK 420
           +MA+NN VGDQDYINVS SRSSDY SRKSSKAKNRD PAMSS  N+IVLLKT+ FSNN+K
Sbjct: 361 EMASNNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQIVLLKTINFSNNHK 420

Query: 421 LCIIISLFLVFPVRVLFHLTFLRYNEDILIYNENMNMSMDLCLLEL--SILFED 473
           LCIIISLFLVFP R LF+L  L YNE+  IY+EN  MS+DLCLLEL  ++LF D
Sbjct: 421 LCIIISLFLVFPARGLFYLIILSYNENTSIYHENTKMSIDLCLLELNMNVLFND 461

BLAST of Clc01G22270 vs. ExPASy TrEMBL
Match: A0A6J1GDW5 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111453267 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 3.6e-197
Identity = 361/473 (76.32%), Postives = 397/473 (83.93%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW RG TIGRGSSAAVS+ATDL SGQ LAVK+VEFT  D L+RE RILS+ NC  VI Y
Sbjct: 20  MEWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTRSDCLKREQRILSRFNCPRVIAY 79

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG LMCNLLMEFAPGGSILD ME AGGRLDEA  Q YTREILSGLEYVH+NG
Sbjct: 80  KGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEARAQFYTREILSGLEYVHTNG 139

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFG AR+VE+VSC NLAGT IFMAPEVARGEKQGFAADV
Sbjct: 140 VVHCDIKCCNILVGEDGIKIADFGCARQVEDVSCRNLAGTLIFMAPEVARGEKQGFAADV 199

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGCAVIQMVTGRAPW NVSDPVAAIY+IGSGDDLPE+P F+S+QGKDFLRRCLIRDPE
Sbjct: 200 WAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPRFISDQGKDFLRRCLIRDPE 259

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSV+ELLKHPFVHEQ+S PKQNSRTPTSILEQ  W TMN+SETVQ     PI PK++R
Sbjct: 260 ERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMNNSETVQI----PIMPKLYR 319

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSI 360
           TPLQRIQQLSEIS  QSS I NW+C +DW+TV+SI +EEE+ E+++FVSVLE+PC SQSI
Sbjct: 320 TPLQRIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQSEFVSVLELPCFSQSI 379

Query: 361 DMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRIVLLKTVCFSNNYK 420
            MAT NGVGDQ YINVS+       SR+SSKAK RDLPAMSS  N+IV L T  FSNN+K
Sbjct: 380 KMATKNGVGDQTYINVSI-------SRESSKAKKRDLPAMSSVSNQIVSLNTNSFSNNHK 439

Query: 421 LCIIISLFLVFPVR-VLFHLTFLRYNEDILIYNENMNMSMDLCLLELSILFED 473
           LCII SLFLVF        +TFLRY E+I IY+EN+ M M+LCL EL+IL  D
Sbjct: 440 LCIISSLFLVFLASGGSIRVTFLRYIENIWIYHENVKMLMNLCLFELNILSGD 481

BLAST of Clc01G22270 vs. ExPASy TrEMBL
Match: A0A5D3CNL0 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006310 PE=3 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 3.0e-191
Identity = 341/407 (83.78%), Postives = 362/407 (88.94%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW+RGRT+GRGSSAAVS+ATDLRSGQ +AVKTVEF+ LDFL+RE RILSQLNCS VIGY
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG+LMCNLLMEFAPGGSILD ME AGGRLDEAT Q YTREILSGL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNILVGEDGIKIADFG ARRVE+V  GNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGCAVIQMVTGRAPWAN+SDP+AAIYRIGSGDDLPEIP  MSEQGKDFLRRCLIRDPE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSVNELLKHPFV EQ+S PKQNSRTPTSILEQ +WDT+ND ET    V+SPIRPKIHR
Sbjct: 241 ERWSVNELLKHPFVQEQKSHPKQNSRTPTSILEQGIWDTVNDPET----VESPIRPKIHR 300

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSI 360
           TPLQRIQQL+E+ST       NWEC EDWITVRSIGL     EEND VSV+E PCSSQS+
Sbjct: 301 TPLQRIQQLNEVSTIG----PNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSV 360

Query: 361 DMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRI 408
           +MA+NN VGDQDYINVS SRSSDY SRKSSKAKNRD PAMSS  N+I
Sbjct: 361 EMASNNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQI 394

BLAST of Clc01G22270 vs. ExPASy TrEMBL
Match: A0A0A0KL63 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G513560 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 8.6e-191
Identity = 340/407 (83.54%), Postives = 362/407 (88.94%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW+RGRTIGRGSSAAVS+ATD+R GQ +AVK+VEF+HLDFL+RE RILSQLNCS VIGY
Sbjct: 1   MEWIRGRTIGRGSSAAVSVATDIRFGQVMAVKSVEFSHLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG+LMCNLLMEFAPGGSILDAME AGGRLDEAT Q YTRE+LSGL+YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEKAGGRLDEATAQFYTREVLSGLQYVHSNG 120

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCNIL+GEDGIKIADFG ARRVEEVS GNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILMGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGCAVIQMVTGR PWAN+SDP+AAIYRIGSGDDLPEIP  MSEQGKDFLRRCLIRDPE
Sbjct: 181 WSVGCAVIQMVTGRVPWANLSDPLAAIYRIGSGDDLPEIPRIMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSVNELLKHPFV EQ+S PKQNSRTPTSIL+Q +WDT+ND ET    V+SPIRPKI R
Sbjct: 241 ERWSVNELLKHPFVQEQKSHPKQNSRTPTSILDQGIWDTVNDPET----VESPIRPKIQR 300

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREENDFVSVLEMPCSSQSI 360
           TPLQRIQQL+E+ST    GI NWEC EDWITVRSIGL     EEND VSV+E P S QSI
Sbjct: 301 TPLQRIQQLNEVST---IGIPNWECDEDWITVRSIGL-----EENDIVSVMETPSSFQSI 360

Query: 361 DMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRI 408
            M TNNGVGDQDYINVS SRSSDY SRKSSKAKNRD PAMSS  N+I
Sbjct: 361 KMETNNGVGDQDYINVSRSRSSDYGSRKSSKAKNRDFPAMSSVFNQI 395

BLAST of Clc01G22270 vs. ExPASy TrEMBL
Match: A0A6J1EMC5 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111433865 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 7.3e-182
Identity = 340/454 (74.89%), Postives = 371/454 (81.72%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW RGR IG GSSAAVS+ATDLRSGQ LAVK+VEF   DF++RE RILSQLN S VI  
Sbjct: 13  MEWTRGRVIGCGSSAAVSVATDLRSGQLLAVKSVEFDRSDFIKREQRILSQLNSSRVIAC 72

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
           KGFDVT ENG+LMCNLLMEFA GGSILDAME AGGRLDEAT Q YTREILSGLEYVHSNG
Sbjct: 73  KGFDVTLENGNLMCNLLMEFASGGSILDAMEAAGGRLDEATAQFYTREILSGLEYVHSNG 132

Query: 121 VVHCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKC NILVGEDGIKIADFG ARRVEEVS GNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 133 VVHCDIKCSNILVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 192

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPE 240
           W VGC VIQMVTGRAPW ++SDPVAAIYRIGS DDLPEIP FMSEQGK+FLRRCLIRDPE
Sbjct: 193 WAVGCTVIQMVTGRAPWEDMSDPVAAIYRIGSSDDLPEIPRFMSEQGKEFLRRCLIRDPE 252

Query: 241 ERWSVNELLKHPFVHEQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPVQSPIRPKIHR 300
           ERWSV+ELLKHPFVHEQ+S+PKQN RTPTSILEQ LW+TMND ET    VQSPIRPK +R
Sbjct: 253 ERWSVSELLKHPFVHEQKSRPKQNPRTPTSILEQELWNTMNDLET----VQSPIRPKFYR 312

Query: 301 TPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGL--EEENREENDFVSVLEMPCSSQ 360
           TPLQRIQQLSE+S  QS GI NWE  EDW+TVRS  +  EEE+RE+ DF+S LE+PCSS+
Sbjct: 313 TPLQRIQQLSEVSKFQSFGIPNWESNEDWVTVRSKDMEEEEEDREKRDFISELELPCSSE 372

Query: 361 SIDMATNNGVGDQDYINVSVSRSSDYYSRKSSKAKNRDLPAMSSACNRIVLLKTVCFSNN 420
           S+   T NG GDQ+YI  S+S+SSDY  +K +         MSS  ++IV L  V F NN
Sbjct: 373 SMATTTQNGAGDQNYI--SISKSSDYCCKKINF-------PMSSVFSQIVSLNIVNFCNN 432

Query: 421 YKLCIIISLFLVFPVRVLFHLTFLRYNEDILIYN 453
            KLC +ISL LVF      HLT  RYNE ILIY+
Sbjct: 433 NKLCYVISLLLVFLSASSVHLTSFRYNEFILIYH 453

BLAST of Clc01G22270 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 296.6 bits (758), Expect = 3.6e-80
Identity = 158/357 (44.26%), Postives = 229/357 (64.15%), Query Frame = 0

Query: 3   WMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGYKG 62
           W+RG  IGRGS+A VS+     SG   AVK+ EF+   FL+RE  ILS+L+   ++ Y G
Sbjct: 6   WIRGPIIGRGSTATVSLGI-TNSGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYIG 65

Query: 63  FDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNGVV 122
            +VT EN  LM NLLME+  GGS+ D ++ +GG+L E  ++ YTR+IL GL Y+H  G+V
Sbjct: 66  SNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGIV 125

Query: 123 HCDIKCCNILVGEDGIKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAADVWG 182
           HCD+K  N+++G +  KI D G A+ VEE      +GTP FM+PEVARGE+Q F ADVW 
Sbjct: 126 HCDVKSQNVMIGGEIAKIVDLGCAKTVEENENLEFSGTPAFMSPEVARGEEQSFPADVWA 185

Query: 183 VGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDPEER 242
           +GC VI+M TG +PW  ++D VAAIY+IG   + P IP ++SE+G+DFLR+CL +DP++R
Sbjct: 186 LGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQR 245

Query: 243 WSVNELLKHPFVHEQRSQPKQ-----NSRTPTSILEQALWDTMNDSET--VQTPVQSPIR 302
           W+V ELL+HPF+ E+ +   Q     NS +P+++L+Q  WD    S +  ++   + P  
Sbjct: 246 WTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWDLCETSRSRFIKEDHEDPFA 305

Query: 303 PKIH------RTPLQRIQQLSEISTNQSSGIANWECGEDWITVRSIGLEEENREEND 347
              +        P  RI++L   + ++SSG  +WE    WI VR   +E+ N EE++
Sbjct: 306 NSTNFLWDDDSLPGDRIKKL---AGDESSGEPDWET-NGWIEVRG-EIEKRNEEEDE 356

BLAST of Clc01G22270 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-77
Identity = 158/352 (44.89%), Postives = 213/352 (60.51%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           MEW RGR +GRGS+A V  A    S + LAVK+ E    +FL+RE +ILS L+   VIGY
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  KGFDVTFE-NGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSN 120
           +G +   E NG +M NLLME+AP G++ DA    GGR+DE  V  YTR+IL GLEY+HS 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GVVHCDIKCCNILVGEDG-IKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAA 180
           G+VHCD+K  N+++ E G  KIADFG A+RV+ V    + GTP FMAPEVARGEKQG  +
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DVWGVGCAVIQMVTGRAPWANV---SDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCL 240
           D+W VGC +I+MVTG  PW       DPV+ +YR+G   + PE+P  ++E+ KDFL +CL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 IRDPEERWSVNELLKHPFVH-----EQRSQPKQNSRTPTSILEQALWDTMNDSETVQTPV 300
            R+  ERW+  +LL HPF+      E    P   S +PTS+ +Q  W ++ + E  +T  
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEE 300

Query: 301 QSPIRPKIHRTPL-----QRIQQLSEISTNQSSGIANWEC---GEDWITVRS 335
                  + R  L     +RI +L  +      G+    C   G DWI VR+
Sbjct: 301 IQKDSRDLDRLSLWGCYSERIGRLKCV-----GGLDGTRCDMEGGDWIMVRA 347

BLAST of Clc01G22270 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 280.8 bits (717), Expect = 2.0e-75
Identity = 160/345 (46.38%), Postives = 214/345 (62.03%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           M W RG+T+GRGS+A VS AT   SG+ LAVK+ EF   +FL+RE +ILS LN   VIGY
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  KGFDVTFENGH-----LMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEY 120
           +G ++T E  H        +LLME+AP G++ D     GG +DEA V  YTR+IL GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 VH-SNGVVHCDIKCCNILVGEDG-IKIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEK 180
           +H S G+ HCDIK  N+LVGE+G  KIADFG A+ VE      + GTP FMAPE ARGE+
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGFAADVWGVGCAVIQMVTGRAPW--ANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFL 240
           QG  +D+W VGC VI+MVTG  PW  A+ +DPV+ +YR+G   +LPE+P  ++EQ KDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 RRCLIRDPEERWSVNELLKHPFVHEQRSQPKQN--SRTPTSILEQALWDTMNDSETVQTP 300
            +CL ++  ERW+ ++LL HPF+  +  +      + +PTS+ +Q  W       +V+  
Sbjct: 241 GKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMFW------RSVEEE 300

Query: 301 VQSPIRPKIHRTPLQRIQQLSEISTNQSSGIANWEC-GEDWITVR 334
           V             +RI  LS I        + W+  GEDWITVR
Sbjct: 301 VSEDRSSWWECHEDERIGVLSWIGHVVVE--STWDLDGEDWITVR 337

BLAST of Clc01G22270 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 280.0 bits (715), Expect = 3.5e-75
Identity = 159/399 (39.85%), Postives = 249/399 (62.41%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSGQRLAVKTVEFTHLDFLEREHRILSQLNCSNVIGY 60
           + W RG  IGRGS+A VSIA    SG+  AVK+ + +    L++E  ILS L+  +++ Y
Sbjct: 3   INWTRGPIIGRGSTATVSIAIS-SSGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKY 62

Query: 61  KGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGRLDEATVQLYTREILSGLEYVHSNG 120
            G  +T E+  L+ N+LME+  GG++ D ++ +GG+L E  ++ YTR+IL+GL Y+H  G
Sbjct: 63  IGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHERG 122

Query: 121 VVHCDIKCCNILVGEDGI-KIADFGSARRVEEVSCGNLAGTPIFMAPEVARGEKQGFAAD 180
           +VHCD+K  N+LV E+G+ KIAD G A+ V++      +GTP FMAPEVARGE+Q F AD
Sbjct: 123 IVHCDLKSHNVLVEENGVLKIADMGCAKSVDK---SEFSGTPAFMAPEVARGEEQRFPAD 182

Query: 181 VWGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPGFMSEQGKDFLRRCLIRDP 240
           VW +GC +I+M+TG +PW  ++D VAA+Y+IG   + P IP ++S++ KDFL+ CL  D 
Sbjct: 183 VWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKAKDFLKNCLKEDQ 242

Query: 241 EERWSVNELLKHPFV-HEQRSQP----KQNSRTPTSILEQALWDTMNDSET--VQTPVQS 300
           ++RW+V ELLKHPF+  ++ SQ     K  + +P+++L+Q  WD+   S++  V    + 
Sbjct: 243 KQRWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTVLDQRFWDSCESSKSHLVSIDHED 302

Query: 301 PIRPKIHR--TPLQRIQQLSEISTNQSSGIANWECGED--WITVRSIGLEE-ENREENDF 360
           P         +P  RI++L   + ++ S + +W+  +D  WI VR    +E E R+ ++ 
Sbjct: 303 PFAEYSESLDSPADRIEKL---AGDEFSSLLDWDTEDDGGWIQVRGEKHKETEKRDGDED 362

Query: 361 VSVLEMPCSSQSIDMATNNGVGDQDYINVSVSRSSDYYS 387
           V  +E   SSQ+I+         +D+I+   S  S+Y S
Sbjct: 363 VICVEATSSSQTIEEV-------EDWISNQDSLFSEYSS 387

BLAST of Clc01G22270 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 217.6 bits (553), Expect = 2.1e-56
Identity = 133/361 (36.84%), Postives = 191/361 (52.91%), Query Frame = 0

Query: 1   MEWMRGRTIGRGSSAAVSIATDLRSG----QRLAVKTVEFTHLDFLEREHRILSQL-NCS 60
           MEW+RG TIG G+ + VS AT  R+       +AVK+ +      L  E  +L  L +C 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  NVIGYKGFDVTFENGHLMCNLLMEFAPGGSILDAMEVAGGR-LDEATVQLYTREILSGLE 120
            +I   G D T ENG  M NLL+E+A  GS+   M+  GG  L E+TV+ +T  +L GL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YVHSNGVVHCDIKCCNILVGEDG-IKIADFGSARRVEEVSCG-----NLAGTPIFMAPEV 180
           ++H+ G  HCDIK  NIL+  DG +KIADFG A RV+           + GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGEKQGFAADVWGVGCAVIQMVTGRAPWA--NVSDPVAAIYRIGSGDDLPEIPGFMSEQ 240
               + G AADVW +GCAV++M +G+  W+    S  ++ + RIG GD+LP+IP  +SE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 GKDFLRRCLIRDPEERWSVNELLKHPFV-------HEQRSQPKQNSR-----TPTSILEQ 300
           GKDFL +C ++DP +RW+   LL H FV       H +    K         +P    E 
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 ALWD--TMNDSETVQTPVQSPIRPKIHRTPLQRIQQLSEISTNQSSGIANWECGEDWITV 334
             WD  T++ + +  +PV+                    + +  S  I +W  G  W+TV
Sbjct: 301 DDWDSFTLDSNPSFDSPVE-------------------RLGSLVSGSIPDWSVGGSWLTV 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883085.16.9e-22781.39mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida][more]
XP_008441103.12.1e-21581.86PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo][more]
XP_023544013.16.1e-19976.96mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pe... [more]
KAG6603788.15.7e-19776.32Mitogen-activated protein kinase kinase kinase 18, partial [Cucurbita argyrosper... [more]
XP_022950078.17.5e-19776.32mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O808882.3e-7644.89Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP52.9e-7446.38Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9SND63.0e-5536.84Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q9FZ364.4e-5139.86Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
O220407.6e-5142.59Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A1S3B3E31.0e-21581.86mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN... [more]
A0A6J1GDW53.6e-19776.32mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=... [more]
A0A5D3CNL03.0e-19183.78Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw... [more]
A0A0A0KL638.6e-19183.54Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G513... [more]
A0A6J1EMC57.3e-18274.89mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
AT5G55090.13.6e-8044.26mitogen-activated protein kinase kinase kinase 15 [more]
AT2G32510.11.7e-7744.89mitogen-activated protein kinase kinase kinase 17 [more]
AT1G05100.12.0e-7546.38mitogen-activated protein kinase kinase kinase 18 [more]
AT4G26890.13.5e-7539.85mitogen-activated protein kinase kinase kinase 16 [more]
AT3G50310.12.1e-5636.84mitogen-activated protein kinase kinase kinase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..254
e-value: 7.7E-75
score: 264.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 5..254
e-value: 4.4E-52
score: 177.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..254
score: 40.484737
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 2..319
e-value: 2.0E-69
score: 236.3
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 7..260
e-value: 2.9E-25
score: 86.5
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 7..195
e-value: 2.4E-14
score: 51.1
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..360
NoneNo IPR availablePANTHERPTHR48011:SF6MAP KINASE KINASE KINASEcoord: 1..360
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 2..254
e-value: 8.36574E-136
score: 389.958
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 121..133
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..32
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc01G22270.2Clc01G22270.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity