Clc01G11270 (gene) Watermelon (cordophanus) v2

Overview
NameClc01G11270
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionGlutamyl-tRNA synthetase
LocationClcChr01: 16879646 .. 16885225 (-)
RNA-Seq ExpressionClc01G11270
SyntenyClc01G11270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATTAAGCTTTTGTCATTTCCGGCCGATACCCCTCCATTCTTCGTCATTGTGGCTGCCAAGCTTTCCGGTATAACTTTTTCTGAGGATTCCTCTCTTGCTTCTGGCTCCGCTCCCACATTTCTCTTTACTAACGGGTATAATCAGATCCCTCTCTCTGCTTATTTTTATCTTCTTTAACTTCTATTAGAGCAAAATGTTTCAGATTCGACCTTCAAAAGATATTCATTTTGATTTATAACAATTGTTTTTCTATAATTCCAAATTTGTTTCTCTCTCTCTGTTTTTTATTTCTTTGCGCCTATTTCTTGAGTTGAAGGTGTGAAGTCATTGTGGTCTGTTTTATGAACATTCAACTCTAGTAGGTTAAGAACAGAATTTTTATTTTATCCAAGATTTATTTGGTACTTTGCATCATGATCTAACCAGATTGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGTTTCAGAGACCCGAAGCAATTAAAAATGTATTTCTGCATAAATCTTAATACATGCTAATTTTCTACACGGGTGAACATTTTTTTTTTAATTGATGAACTTATAATAGTTATTTGAACTTGATTGAACTGAACCATAAAATCATTGATGTATATACAGAATTTGTATACATGGGCCTATTGAGCAAATTTATAATATCGACTAGGGGATTAACTTAACTTCTGCGGTTTAGTTATATTTTACCAGAATCAGCATACAAGTTGAAGATAAAATTAAGATATTGTTAAGGACAATGGAACCCTCTCAATTGTCTACTTGTCGTGACTTTGCTTTTGATTTCAACCAAAAGGTTTTATATCGATAAAAATATAGTCTCTCATCCATTTACTCTTGATATTCCCTTCTCCAGCTAACGTGGGACTTTGGTTGCATTCTCAACAAAAATGTTGATTCTAACCTTGTCTTCCCTTAATTACTTTCTCTGCCTTTAACTTGAAAACCATGCTCATTATAAATGTTCTTGTTTTATATGCTCTCTGTTTAATCCATTGGCCTAGAGGGAGCCTCTATCTGTCTTGCTTATTATCTTCTCATCTTTCAGATTTGCCAGCTTCTTGTACATGGAATTTATCCTCCAAACTTTTATAGTCTTCTTAAATGCAATTTATAATTGAACCACAAATGTACCACAATTTTCGGACAATCACTTTATTGCATGAATACACTGTTTATGCCTTGACATCCATTGGTGCTTTATTGGTTGTCTTATAAAAAACATGGGGGAATGCATCATTTGTTGTCATTGTCTGCTTACTTTTATTTTATTTTTTTAATAGTTTATGAGTTGGAATTTGACTTTTGCCGAGGCAACATTAGTGATTATTTTAAAGCTTGCTTGGACAACCTTTGATCCAATTGTAAATCATTTCCACTAAACTTTTATCTTTGGAGGCTTGCATTACACTCCTGCTATTTAAACTTCAAAAGGAAATTTACTCTCCATTTGATATGAATCAGAGGAAAGAATGTGTAATAAACTGTTACTCAATGCTTAAGTCTCGTTAATCTGTTGTAGGTCGAAGTTGTATGGAACATCTGTGCTTCTACGTTATATTGGTCGAGTCACTAACATTCCTAATTTCTATGGACCGAACGCATACGAGTCCAGTCAGGTAAGATGAAAAATATATTTATTAACACGATCTAAATGTTCATTCTGATGTATGGCTTGATGTTTATTAATTTTTGCGATTGTTCATAAGGCTAATTTCTTAATAATAAACTTCTCTCATATCTACGCATGGATTTTAACCCAGATTGATGAATGGCTAGAGTATGCTCCTATCCTTTCGTCCGGTTGGTTCTGCATTTGAGAGCGCATGCAGTTATGTTGATAAGTACTTGGAAAGACAGACATTTCTTGTTAGCAATTATTTTTCACTTGCAGATGTAGCTATCTGGTCCGGCTTAGCAGGTATATATGTATATATATCTATATTAAAAATAAAGAACAGTAATTACCAGTGTTTTAAAAAGCCGTTGTGCGTCTAGGCTCAAGGTGCAGGTTTGACACCTTGCCTTGAAAATGCGAGGCTTAGAAAATATGACGCACCTCAAGCGCACGCCTTTTGTGAAGTAGAGCCTTGGGTTTTCTTTCACTACCTTTTAAAAACATAGTAATAATTAGGGTTTTTCCTTCTTTATCAATTAAAAATGTCAAATTTACTGAGCCTAAATGCAAAATTTCTTTTGTGAGTGTCTGTTTTTAATATAATTTTTAATTTTATATATTTCCACTTCAAATATTCTTTCTCTACTTTAAGTAATACTTTTATATGTATTGCGCCTCACAAACAAAACCCACTTTTTTCTCCAAGCACTTGAACTCCATAAGACTATTCCACTTTGAGCTTTAAATAACATAGGTAGCTACCATTTATCCATTGTTTCTCAAATCTTTTCCTACATCACCAAGCCTATCTCGAGAGAGGGAGAGAAAGACAAGATGCCATGTCGTGTTTGTTTTTATTAAATTAATCATAGTGTTCCTTGTTTTTAACCCACATGCCTACTTATACACAGACACAGTCACAATCACACACAAACGCTCCATATATAGAGTGACAGATAGTTGGCTTATTAAAACTATGTGCATTGTGTTTTTTACAGGAACTGGGCAACGGTGGGAAAGCTTGAGAAAGTCAAAGAAGCACATAAATCTTCAACGATGGTTTAATTCACTCTTAGTTGAATATCGTGATAACTTGGATGATGTCTTAACTACATATGCTGGCAAAAGAGGTGGAAAGGCTTCAGGAGCTAAAAGCCAAATAGTGTCAACCACGAGACATCTGATAAGGGGAAAGCTGGAAGCAAATGTACCTTTGAAGTTGATCTTCCAAATGTGGAGATCGGGAAGGTGCGTTTGCGTTTTGCACCTGAGCCTAGTGGATATCTTCACATTGGGCACTCAAAAGCAGCATTGTTGAATCAGTATTTTGCCCAAAGGTACAATGGTGAAGTAATCATCAGGTTTGATGATACAAATCTTGCTAAAGAAAGCAATGAGTTTGTTGAAAATCTTTTGAAAGACATTGAGACATTGGGTATAAAGTATGAAACTGTCACATATACTTCAGATTACTTCCCTCAATTAATGGAAATGGCTGAAAACTTAATTCGTCAGGGTAAAGCATATATTGATGATACTCCACGTGAGCAGATGCAAAAAGAACGAATGGAAGGCATTGAATCAAAAAATAGAAGTAATAGTGCCGAAGAGAATCTAAGATTATGGAAGGAGATGGTATTAGGAACTGAGCAGGGTTTACTTTGTTGTCTTCGCGGAAAACTTGATATGCAAGACCCTAATAAATCTCTTCGAGATCCAGTGTATTACCGTTGTAATCCAATTCCCCACCATCGGATTGGTTCCAAGTATAGGATATACCCAACTTATGATTTTGCCTGTCCGTTTGTAGATTCTATTGAAGGTATTACCCATGCACTTCGGTCTAGCGAATACCATGACCGTAATGCCCAGTACTACCGAATTCAGGGAGATATGGGTCTAAGAAAGGTTCATATATATGAATTTAGCCGGTTGAACATGGTGTATACTCTTCTCAGCAAGCGTAAGCTTTTGTGGTTTGTTCAAAATGGGAAGGTCGATGGGTGGGATGATCCTCGTTTTCCAACAGTTCAGGGGATTGTTCGAAGAGGTTTAAAAGTTGAAGCTTTAATTCAATTCATCCTTGAACAGGTGCGACAATTTTGGTTACAAATAAAATCCTTTAGTTTGTGTTTATTCAAAAGTCAGTGATAAATCTTGCTCTAGTAGCACCAAAGCTGGTATGCACTTGTAATAGTTGTCCTCCCAAAAGGATTTTATTTGTCACAAGCCTCAGTGAATGCCATAAATTTATGCTTAAAAACTTGATTTTCATTTCTATAGAAGATCAAGGTATTTTATGATGATCCTTGTATACTGCATCTGTCATTTTTATATTTCAAATGACAGATATCATGGTCTTTTCAGTTTTTGGCTAGGAAACTAGGTCTCTATGACATGCATCTTTATGAGAGTTGCTCATCTTTTTCTTTTCTTTATTTCAGGGTGCATCAAAAAATCTCAACCTTATGGAATGGGATAAACTATGGACCATTAATAAAAAAGATTATTGATCCTGTGTGTCCAAGACATACTGCTGTTATAGAAGAACGACGTGTGTTATTTACCCTTGAAAATGGTCCAGAGATACCATTTATCCGAGTAATACCAAAGCATAAAAAATATGAAGGTGCTGGTGAAAAATCAACCACTTTCACAAAGAGGATATGGATCGACCAAGTTGATGCACAATGTATCAAGGTGGATGAAGAAATAACCTTAATGGATTGGGGTAATGCAATAGTGAAAGGGATCGAGAAGGATCAAGATGGATTTGTCACACAGTTGTTAGGAATTTTGCATCTCGAAGGTTCTGTGAAGACCACAAAGTTGAAGGCCACTTGGCTACCTGATATAAACGAACTAGTCACCCTCTCACTTATGGAATATGATTATTTGATCACAAAAAAGAAGGTATGGACGTTTAATATGTTGTGGTATCATATGCACTTTTCTTACTAGTAAATGATGTGAGTCCGTTTGTTTGGTTGATTGTGATATTTTATTCTCTTTCCTGTTGGTTTCGAAGATCTGAAGGAGAGATGTGGCTTAAAATCAAAGTTCAAAACTATGCATACTGACATCAGTGTATTTGGATTGAGATTTTTGTGTTTTATTTGTCTTCTATTTTTAAAAGCCAGATTGCAATCTACATCACTTAGCTTGTTTGGGGCATGTTCGGGAGTGATTTTGAAATGGTTTTAATCACTTTTGTAATTTTCATAATCACTCTAAAAAGTGCTTTTAATCATTTAAAACCAATTTTGATGATATGGAAATTGCATTTAAACGTGCAAAATTAAATATTAAATAAGTTGAGTGATTAAAGACGTGTTTTAAGGGTGATTTTGAGCATGACAAAAGTGATTTTAACAATTTTAAAATCACTACCAAAACCCTGCATTTAATGAATAGTTTGGATCATATATTTACGTGCAAGCGGGTAAGCCAGTTTGTCAATGTATGGATGATGTTCAGTTCAAAAGATTTATGAAATTAGATGTTAATGTGAGCTATGTTACATGTGTTAAAAGTTGTTGGTGAACCTGTTCATATGTTGTTAGGAGAGTGGTTATAGAAATTATAAATTTTAGCTTGATATATACAAATTGCTATTACAGCTGGAAGAAGGAGAGGATTTTCTTGATGTATTAAATCCATGCACAAAAAAGGAGACTGTTGTTGTTGGGGATTCAAACATGCGAAATATAAAGCGTGGAGACATATTGCAGTTAGAGAGGAAGGGGTATTTCAGATGCGATGTTCCATATGTAAGGCCATCCAAACCGATAGTCCTATTTGCAATCCCAGATGGCAGGCAACAATCTTCTTTGAAGTAAAATCTCTGTTTTTTTTTTAGTGTTTCTCTAGGTGATCATTCTTACATCTACCTGTTATAATCTATGATATACATATATATATCTTTTTTTTTGG

mRNA sequence

ATGGATATTAAGCTTTTGTCATTTCCGGCCGATACCCCTCCATTCTTCGTCATTGTGGCTGCCAAGCTTTCCGGTATAACTTTTTCTGAGGATTCCTCTCTTGCTTCTGGCTCCGCTCCCACATTTCTCTTTACTAACGGGTCGAAGTTGTATGGAACATCTGTGCTTCTACGTTATATTGGTCGAGTCACTAACATTCCTAATTTCTATGGACCGAACGCATACGAGTCCAGTCAGATTGATGAATGGCTAGAGTATGCTCCTATCCTTTCGTCCGGAACTGGGCAACGGTGGGAAAGCTTGAGAAAGTCAAAGAAGCACATAAATCTTCAACGATGGTTTAATTCACTCTTAGTTGAATATCGTGATAACTTGGATGATGTCTTAACTACATATGCTGGCAAAAGAGGTGGAAAGGCTTCAGGAGCTAAAAGCCAAATAGTGTCAACCACGAGACATCTGATAAGGGGAAAGCTGGAAGCAAATATCGGGAAGGTGCGTTTGCGTTTTGCACCTGAGCCTAGTGGATATCTTCACATTGGGCACTCAAAAGCAGCATTGTTGAATCAGTATTTTGCCCAAAGGTACAATGGTGAAGTAATCATCAGGTTTGATGATACAAATCTTGCTAAAGAAAGCAATGAGTTTGTTGAAAATCTTTTGAAAGACATTGAGACATTGGGTATAAAGTATGAAACTGTCACATATACTTCAGATTACTTCCCTCAATTAATGGAAATGGCTGAAAACTTAATTCGTCAGGGTAAAGCATATATTGATGATACTCCACGTGAGCAGATGCAAAAAGAACGAATGGAAGGCATTGAATCAAAAAATAGAAGTAATAGTGCCGAAGAGAATCTAAGATTATGGAAGGAGATGGTATTAGGAACTGAGCAGGGTTTACTTTGTTGTCTTCGCGGAAAACTTGATATGCAAGACCCTAATAAATCTCTTCGAGATCCAGTGTATTACCGTTGTAATCCAATTCCCCACCATCGGATTGGTTCCAAGTATAGGATATACCCAACTTATGATTTTGCCTGTCCGTTTGTAGATTCTATTGAAGGTATTACCCATGCACTTCGGTCTAGCGAATACCATGACCGTAATGCCCAGTACTACCGAATTCAGGGAGATATGGGTCTAAGAAAGGTTCATATATATGAATTTAGCCGGTTGAACATGGTGTATACTCTTCTCAGCAAGCGTAAGCTTTTGTGGTTTGTTCAAAATGGGAAGGTCGATGGGTGGGATGATCCTCGTTTTCCAACAGTTCAGGGGATTGTTCGAAGAGGTTTAAAAGTTGAAGCTTTAATTCAATTCATCCTTGAACAGATTATTGATCCTGTGTGTCCAAGACATACTGCTGTTATAGAAGAACGACGTGTGTTATTTACCCTTGAAAATGGTCCAGAGATACCATTTATCCGAGTAATACCAAAGCATAAAAAATATGAAGGTGCTGGTGAAAAATCAACCACTTTCACAAAGAGGATATGGATCGACCAAGTTGATGCACAATGTATCAAGGTGGATGAAGAAATAACCTTAATGGATTGGGGTAATGCAATAGTGAAAGGGATCGAGAAGGATCAAGATGGATTTGTCACACAGTTGTTAGGAATTTTGCATCTCGAAGGTTCTGTGAAGACCACAAAGTTGAAGGCCACTTGGCTACCTGATATAAACGAACTAGTCACCCTCTCACTTATGGAATATGATTATTTGATCACAAAAAAGAAGCTGGAAGAAGGAGAGGATTTTCTTGATGTATTAAATCCATGCACAAAAAAGGAGACTGTTGTTGTTGGGGATTCAAACATGCGAAATATAAAGCGTGGAGACATATTGCAGTTAGAGAGGAAGGGGTATTTCAGATGCGATGTTCCATATGTAAGGCCATCCAAACCGATAGTCCTATTTGCAATCCCAGATGGCAGGCAACAATCTTCTTTGAAGTAAAATCTCTGTTTTTTTTTTAGTGTTTCTCTAGGTGATCATTCTTACATCTACCTGTTATAATCTATGATATACATATATATATCTTTTTTTTTGG

Coding sequence (CDS)

ATGGATATTAAGCTTTTGTCATTTCCGGCCGATACCCCTCCATTCTTCGTCATTGTGGCTGCCAAGCTTTCCGGTATAACTTTTTCTGAGGATTCCTCTCTTGCTTCTGGCTCCGCTCCCACATTTCTCTTTACTAACGGGTCGAAGTTGTATGGAACATCTGTGCTTCTACGTTATATTGGTCGAGTCACTAACATTCCTAATTTCTATGGACCGAACGCATACGAGTCCAGTCAGATTGATGAATGGCTAGAGTATGCTCCTATCCTTTCGTCCGGAACTGGGCAACGGTGGGAAAGCTTGAGAAAGTCAAAGAAGCACATAAATCTTCAACGATGGTTTAATTCACTCTTAGTTGAATATCGTGATAACTTGGATGATGTCTTAACTACATATGCTGGCAAAAGAGGTGGAAAGGCTTCAGGAGCTAAAAGCCAAATAGTGTCAACCACGAGACATCTGATAAGGGGAAAGCTGGAAGCAAATATCGGGAAGGTGCGTTTGCGTTTTGCACCTGAGCCTAGTGGATATCTTCACATTGGGCACTCAAAAGCAGCATTGTTGAATCAGTATTTTGCCCAAAGGTACAATGGTGAAGTAATCATCAGGTTTGATGATACAAATCTTGCTAAAGAAAGCAATGAGTTTGTTGAAAATCTTTTGAAAGACATTGAGACATTGGGTATAAAGTATGAAACTGTCACATATACTTCAGATTACTTCCCTCAATTAATGGAAATGGCTGAAAACTTAATTCGTCAGGGTAAAGCATATATTGATGATACTCCACGTGAGCAGATGCAAAAAGAACGAATGGAAGGCATTGAATCAAAAAATAGAAGTAATAGTGCCGAAGAGAATCTAAGATTATGGAAGGAGATGGTATTAGGAACTGAGCAGGGTTTACTTTGTTGTCTTCGCGGAAAACTTGATATGCAAGACCCTAATAAATCTCTTCGAGATCCAGTGTATTACCGTTGTAATCCAATTCCCCACCATCGGATTGGTTCCAAGTATAGGATATACCCAACTTATGATTTTGCCTGTCCGTTTGTAGATTCTATTGAAGGTATTACCCATGCACTTCGGTCTAGCGAATACCATGACCGTAATGCCCAGTACTACCGAATTCAGGGAGATATGGGTCTAAGAAAGGTTCATATATATGAATTTAGCCGGTTGAACATGGTGTATACTCTTCTCAGCAAGCGTAAGCTTTTGTGGTTTGTTCAAAATGGGAAGGTCGATGGGTGGGATGATCCTCGTTTTCCAACAGTTCAGGGGATTGTTCGAAGAGGTTTAAAAGTTGAAGCTTTAATTCAATTCATCCTTGAACAGATTATTGATCCTGTGTGTCCAAGACATACTGCTGTTATAGAAGAACGACGTGTGTTATTTACCCTTGAAAATGGTCCAGAGATACCATTTATCCGAGTAATACCAAAGCATAAAAAATATGAAGGTGCTGGTGAAAAATCAACCACTTTCACAAAGAGGATATGGATCGACCAAGTTGATGCACAATGTATCAAGGTGGATGAAGAAATAACCTTAATGGATTGGGGTAATGCAATAGTGAAAGGGATCGAGAAGGATCAAGATGGATTTGTCACACAGTTGTTAGGAATTTTGCATCTCGAAGGTTCTGTGAAGACCACAAAGTTGAAGGCCACTTGGCTACCTGATATAAACGAACTAGTCACCCTCTCACTTATGGAATATGATTATTTGATCACAAAAAAGAAGCTGGAAGAAGGAGAGGATTTTCTTGATGTATTAAATCCATGCACAAAAAAGGAGACTGTTGTTGTTGGGGATTCAAACATGCGAAATATAAAGCGTGGAGACATATTGCAGTTAGAGAGGAAGGGGTATTTCAGATGCGATGTTCCATATGTAAGGCCATCCAAACCGATAGTCCTATTTGCAATCCCAGATGGCAGGCAACAATCTTCTTTGAAGTAA

Protein sequence

MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYIGRVTNIPNFYGPNAYESSQIDEWLEYAPILSSGTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASGAKSQIVSTTRHLIRGKLEANIGKVRLRFAPEPSGYLHIGHSKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPTVQGIVRRGLKVEALIQFILEQIIDPVCPRHTAVIEERRVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWGNAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKKLEEGEDFLDVLNPCTKKETVVVGDSNMRNIKRGDILQLERKGYFRCDVPYVRPSKPIVLFAIPDGRQQSSLK
Homology
BLAST of Clc01G11270 vs. NCBI nr
Match: XP_038895456.1 (glutamate--tRNA ligase, cytoplasmic-like [Benincasa hispida] >XP_038895457.1 glutamate--tRNA ligase, cytoplasmic-like [Benincasa hispida])

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 609/735 (82.86%), Postives = 629/735 (85.58%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIK+LSFP DT PF VIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKL+GT VLLRYI
Sbjct: 1   MDIKVLSFPGDTAPFSVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLHGTFVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILSS---------------------------- 120
           GRV+NIPNFYG NAYESSQIDEWLEYAPILSS                            
Sbjct: 61  GRVSNIPNFYGQNAYESSQIDEWLEYAPILSSGSAFESACSYVDKYLERRTFLVSHSLSL 120

Query: 121 ----------GTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                     GTGQRWESLRKS K++NLQRWFNSL+VEYRDNLDDVLTTY GKRGGKASG
Sbjct: 121 ADVAIWSGLAGTGQRWESLRKSTKYLNLQRWFNSLIVEYRDNLDDVLTTYVGKRGGKASG 180

Query: 181 AKSQIVSTTRHLI------------RGKL-----------EANIGKVRLRFAPEPSGYLH 240
           AK   +   + LI            +GK+              IGKVRLRFAPEPSGYLH
Sbjct: 181 AK---LKDPQGLIQKPNGVNQDASDKGKVGSKSTFEVDLPNVEIGKVRLRFAPEPSGYLH 240

Query: 241 IGHSKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSD 300
           IGHSKAALLNQYFAQRY+GEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSD
Sbjct: 241 IGHSKAALLNQYFAQRYDGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSD 300

Query: 301 YFPQLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTE 360
           YFPQLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNS EENLRLWKEMVLGTE
Sbjct: 301 YFPQLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSPEENLRLWKEMVLGTE 360

Query: 361 QGLLCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGIT 420
           QGLLCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKY+IYPTYDFACPFVDSIEGIT
Sbjct: 361 QGLLCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYKIYPTYDFACPFVDSIEGIT 420

Query: 421 HALRSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWD 480
           HALRSSEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWD
Sbjct: 421 HALRSSEYHDRNAQYYRIQKDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWD 480

Query: 481 DPRFPTVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVI 540
           DPRFPTVQGIVRRGLKVEALIQFILEQ                    IIDPVCPRHTAVI
Sbjct: 481 DPRFPTVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVI 540

Query: 541 EERRVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLM 600
           EERRVL TL+NGP++PF+RVIPKHKKYEGAGEKSTTFTK+IWIDQVDAQCIK+DEEITLM
Sbjct: 541 EERRVLLTLDNGPDVPFVRVIPKHKKYEGAGEKSTTFTKKIWIDQVDAQCIKMDEEITLM 600

Query: 601 DWGNAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLIT 655
           DWGNAIVKGIEKDQDGFVTQL GILHLEGSVKTTKLK TWLPDINELVTLSLME+DYLI 
Sbjct: 601 DWGNAIVKGIEKDQDGFVTQLSGILHLEGSVKTTKLKVTWLPDINELVTLSLMEFDYLIA 660

BLAST of Clc01G11270 vs. NCBI nr
Match: XP_022961381.1 (glutamate--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022961382.1 glutamate--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022961383.1 glutamate--tRNA ligase, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 604/732 (82.51%), Postives = 624/732 (85.25%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKLLSFPAD+PP  VIVAAK SGITF  DSSLASGSAPTFLFTNG KL+GTSVLLRYI
Sbjct: 1   MDIKLLSFPADSPPLSVIVAAKFSGITFPADSSLASGSAPTFLFTNGLKLHGTSVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILS----------------------------- 120
           GRVTNIPNFYG NAYESSQIDEWLEYAPILS                             
Sbjct: 61  GRVTNIPNFYGENAYESSQIDEWLEYAPILSTGSAFENACSYVDKYLERRTFLVSHSLSL 120

Query: 121 ---------SGTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                    +GTG RWESLRKSKK++NLQRWFNSLLVEY D LDDVLTTY GKRGGK+SG
Sbjct: 121 ADVAIWSGLAGTGPRWESLRKSKKYLNLQRWFNSLLVEYSDGLDDVLTTYVGKRGGKSSG 180

Query: 181 AK---SQIVSTTRHLI------RGKL-----------EANIGKVRLRFAPEPSGYLHIGH 240
           AK    Q ++   + +      +GK             A IGKVRLRFAPEPSGYLHIGH
Sbjct: 181 AKLKDQQGLTQKPNSVNQDTSDKGKSGSKSTFEVDLPNAEIGKVRLRFAPEPSGYLHIGH 240

Query: 241 SKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300
           SKAALLNQYFAQRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYFP
Sbjct: 241 SKAALLNQYFAQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300

Query: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGL 360
           QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNS EENLRLWKEM+LGTE+GL
Sbjct: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSPEENLRLWKEMILGTEKGL 360

Query: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHAL 420
           LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKY+IYPTYDFACPFVDSIEGITHAL
Sbjct: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYKIYPTYDFACPFVDSIEGITHAL 420

Query: 421 RSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480
           RSSEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR
Sbjct: 421 RSSEYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480

Query: 481 FPTVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEER 540
           FPTVQGIVRRGLKVEALIQFILEQ                    IIDPVCPRHTAVIEER
Sbjct: 481 FPTVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVIEER 540

Query: 541 RVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWG 600
           RVLFTL+NGPE PF+RVIPKHKKYEGAGEKS+TFTKRIWIDQVDAQCIKVDEEITLMDWG
Sbjct: 541 RVLFTLDNGPEKPFVRVIPKHKKYEGAGEKSSTFTKRIWIDQVDAQCIKVDEEITLMDWG 600

Query: 601 NAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 655
           NAI+KGIEKD DGFVT+L GILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK
Sbjct: 601 NAILKGIEKDGDGFVTRLSGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 660

BLAST of Clc01G11270 vs. NCBI nr
Match: KAG6590522.1 (Glutamate--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 602/732 (82.24%), Postives = 623/732 (85.11%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKLLSFPAD+PP  VIVAAK + ITF  DSSLASGSAPTFLFTNG KL+GTSVLLRYI
Sbjct: 1   MDIKLLSFPADSPPLSVIVAAKFASITFPADSSLASGSAPTFLFTNGLKLHGTSVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILS----------------------------- 120
           GRVTNIPNFYG NAYESSQIDEWLEYAPILS                             
Sbjct: 61  GRVTNIPNFYGENAYESSQIDEWLEYAPILSTGSAFENACSYVDKYLERRTFLVSHSLSL 120

Query: 121 ---------SGTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                    +GTG RWESLRKSKK++NLQRWFNSLLVEY D LDDVLTTY GKRGGK+SG
Sbjct: 121 ADVAIWSGLAGTGPRWESLRKSKKYLNLQRWFNSLLVEYSDGLDDVLTTYVGKRGGKSSG 180

Query: 181 AK---SQIVSTTRHLI------RGKL-----------EANIGKVRLRFAPEPSGYLHIGH 240
           AK    Q ++   + +      +GK             A IGKVRLRFAPEPSGYLHIGH
Sbjct: 181 AKLKDQQGLTQKPNSVNQDTSDKGKSGSKSTFEVDLPNAEIGKVRLRFAPEPSGYLHIGH 240

Query: 241 SKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300
           SKAALLNQYFAQRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYFP
Sbjct: 241 SKAALLNQYFAQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300

Query: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGL 360
           QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNS EENLRLWKEM+LGTE+GL
Sbjct: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSPEENLRLWKEMILGTEKGL 360

Query: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHAL 420
           LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKY+IYPTYDFACPFVDSIEGITHAL
Sbjct: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYKIYPTYDFACPFVDSIEGITHAL 420

Query: 421 RSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480
           RSSEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR
Sbjct: 421 RSSEYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480

Query: 481 FPTVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEER 540
           FPTVQGIVRRGLKVEALIQFILEQ                    IIDP+CPRHTAVIEER
Sbjct: 481 FPTVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPMCPRHTAVIEER 540

Query: 541 RVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWG 600
           RVLFTL+NGPE PF+RVIPKHKKYEGAGEKS+TFTKRIWIDQVDAQCIKVDEEITLMDWG
Sbjct: 541 RVLFTLDNGPEKPFVRVIPKHKKYEGAGEKSSTFTKRIWIDQVDAQCIKVDEEITLMDWG 600

Query: 601 NAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 655
           NAIVKGIEKD DGFVT+L GILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK
Sbjct: 601 NAIVKGIEKDGDGFVTRLSGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 660

BLAST of Clc01G11270 vs. NCBI nr
Match: XP_023521072.1 (glutamate--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] >XP_023521073.1 glutamate--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] >XP_023521074.1 glutamate--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 602/732 (82.24%), Postives = 622/732 (84.97%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKLLSFPAD+PP  VIVAAK + ITF  DSSLASGSAPTFLFTNGSKL+GTSVLLRYI
Sbjct: 1   MDIKLLSFPADSPPLSVIVAAKFASITFPADSSLASGSAPTFLFTNGSKLHGTSVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILS----------------------------- 120
           GRVTNIPNFYG NAYESSQIDEWLEYAPILS                             
Sbjct: 61  GRVTNIPNFYGENAYESSQIDEWLEYAPILSTGSAFENACSYVDKYLERRTFLVSHSLSL 120

Query: 121 ---------SGTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                    +GTG RWESLRKSKK++NLQRWFNSLLVEY D LDDVLTTY GKRGGK+SG
Sbjct: 121 ADVAIWSGLAGTGPRWESLRKSKKYLNLQRWFNSLLVEYSDELDDVLTTYVGKRGGKSSG 180

Query: 181 AK---SQIVSTTRHLI------RGKL-----------EANIGKVRLRFAPEPSGYLHIGH 240
           AK    Q ++   + +      +GK             A IGKVRLRFAPEPSGYLHIGH
Sbjct: 181 AKLKDQQGLTQKPNSVNQDTYDKGKSGSKSTFEVDLPNAEIGKVRLRFAPEPSGYLHIGH 240

Query: 241 SKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300
           SKAALLNQYFAQRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYFP
Sbjct: 241 SKAALLNQYFAQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300

Query: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGL 360
           QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNS EENLRLWKEM+LGTE+GL
Sbjct: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSPEENLRLWKEMILGTEKGL 360

Query: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHAL 420
           LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSK++IYPTYDFACPFVDSIEGITHAL
Sbjct: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKFKIYPTYDFACPFVDSIEGITHAL 420

Query: 421 RSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480
           RSSEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNG VDGWDDPR
Sbjct: 421 RSSEYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGMVDGWDDPR 480

Query: 481 FPTVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEER 540
           FPTVQGIVRRGLKVEALIQFILEQ                    IIDPVCPRHTAVIEER
Sbjct: 481 FPTVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVIEER 540

Query: 541 RVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWG 600
           RVLFTL+NGPE PF+RVIPKHKKYEGAGEKS+TFTKRIWIDQVDAQCIKVDEEITLMDWG
Sbjct: 541 RVLFTLDNGPEKPFVRVIPKHKKYEGAGEKSSTFTKRIWIDQVDAQCIKVDEEITLMDWG 600

Query: 601 NAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 655
           NAIVK IEKD DGFVT+L GILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK
Sbjct: 601 NAIVKCIEKDGDGFVTRLSGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 660

BLAST of Clc01G11270 vs. NCBI nr
Match: XP_038878933.1 (glutamate--tRNA ligase, cytoplasmic [Benincasa hispida])

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 600/732 (81.97%), Postives = 622/732 (84.97%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKLLSFPADTPP  VIVAAKL+  T   DSSL S SAPTFLFTNGSKL+GTSVLLRYI
Sbjct: 1   MDIKLLSFPADTPPLSVIVAAKLARFTIPVDSSLPSASAPTFLFTNGSKLHGTSVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILSS---------------------------- 120
           GRVTNIPNFYG NAYESSQIDEWLEYAPILSS                            
Sbjct: 61  GRVTNIPNFYGQNAYESSQIDEWLEYAPILSSGSAFENACSYVDKYLERRTFLVSHSLSL 120

Query: 121 ----------GTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                     GTGQRWESLRKSKK++NLQRWFNSLLVEY ++LDDVLTTY GK+GGK+SG
Sbjct: 121 ADVAVWAGLAGTGQRWESLRKSKKYLNLQRWFNSLLVEYSNDLDDVLTTYVGKKGGKSSG 180

Query: 181 AKSQ----IVSTTRHL-----IRGK-----------LEANIGKVRLRFAPEPSGYLHIGH 240
           AK +    ++  T  +      +GK               IGKVRLRFAPEPSGYLHIGH
Sbjct: 181 AKLKDIQGLIQKTNSVNQDTSDKGKPGSKSTFEVDLPNVEIGKVRLRFAPEPSGYLHIGH 240

Query: 241 SKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300
           SKAALLNQYFAQRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYFP
Sbjct: 241 SKAALLNQYFAQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300

Query: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGL 360
           QLMEMAENLI QGKAY+DDTPREQMQKERMEGIESK+RSNS EENLRLWKEM+LGTEQGL
Sbjct: 301 QLMEMAENLIHQGKAYVDDTPREQMQKERMEGIESKSRSNSPEENLRLWKEMILGTEQGL 360

Query: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHAL 420
           LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKY+IYPTYDFACPFVDSIEGITHAL
Sbjct: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYKIYPTYDFACPFVDSIEGITHAL 420

Query: 421 RSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480
           RSSEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQ+GKVDGWDDPR
Sbjct: 421 RSSEYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQHGKVDGWDDPR 480

Query: 481 FPTVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEER 540
           FPTVQGIVRRGLKVEALIQFILEQ                    IIDPVCPRHTAVIEER
Sbjct: 481 FPTVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVIEER 540

Query: 541 RVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWG 600
           RVLFTL+NGPE PFIR+IPKHKKYEGAGEKS+TFTKRIWIDQVDAQCIKVDEEITLMDWG
Sbjct: 541 RVLFTLDNGPETPFIRIIPKHKKYEGAGEKSSTFTKRIWIDQVDAQCIKVDEEITLMDWG 600

Query: 601 NAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 655
           NAIVKGIEKDQDGFVTQL GILH EGSVKTTKLKATWLPDI+ELVTLSLMEYDYLITKKK
Sbjct: 601 NAIVKGIEKDQDGFVTQLSGILHPEGSVKTTKLKATWLPDIDELVTLSLMEYDYLITKKK 660

BLAST of Clc01G11270 vs. ExPASy Swiss-Prot
Match: O82462 (Glutamate--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g26710 PE=1 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 3.0e-271
Identity = 462/718 (64.35%), Postives = 554/718 (77.16%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MD   LSFP ++PP  VIVA  LS    + DSS A+ + P+F+F++G KL G +VLLRY+
Sbjct: 1   MDGMKLSFPPESPPLSVIVALSLSASPVTIDSSAAATTVPSFVFSDGRKLNGATVLLRYV 60

Query: 61  GR-VTNIPNFYGPNAYESSQIDEWLEYAPILSS--------------------------- 120
           GR    +P+FYG NA++SSQIDEW++YA + SS                           
Sbjct: 61  GRSAKKLPDFYGNNAFDSSQIDEWVDYASVFSSGSEFENACGRVDKYLESSTFLVGHSLS 120

Query: 121 -----------GTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKAS 180
                      GTGQRWESLRKSKK+ +L RWFNS+L EY + L+ VL TY  K  GK  
Sbjct: 121 IADVAIWSALAGTGQRWESLRKSKKYQSLVRWFNSILDEYSEVLNKVLATYVKKGSGKPV 180

Query: 181 GAKSQIVSTTRHLIRGK-----------LEANIGKVRLRFAPEPSGYLHIGHSKAALLNQ 240
            A       ++  ++G             EA IGKV+LRFAPEPSGYLHIGH+KAALLN+
Sbjct: 181 AAPKS--KDSQQAVKGDGQDKGKPEVDLPEAEIGKVKLRFAPEPSGYLHIGHAKAALLNK 240

Query: 241 YFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLMEMAEN 300
           YFA+RY GEVI+RFDDTN AKESNEFV+NL+KDI TLGIKYE VTYTSDYFP+LM+MAE 
Sbjct: 241 YFAERYQGEVIVRFDDTNPAKESNEFVDNLVKDIGTLGIKYEKVTYTSDYFPELMDMAEK 300

Query: 301 LIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLCCLRGKL 360
           L+R+GKAY+DDTPREQMQKERM+GI+SK R++S EENL+LWKEM+ G+E+GL CC+RGK 
Sbjct: 301 LMREGKAYVDDTPREQMQKERMDGIDSKCRNHSVEENLKLWKEMIAGSERGLQCCVRGKF 360

Query: 361 DMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRSSEYHDR 420
           +MQDPNK++RDPVYYRCNP+ HHRIG KY+IYPTYDFACPFVDS+EGITHALRSSEYHDR
Sbjct: 361 NMQDPNKAMRDPVYYRCNPMSHHRIGDKYKIYPTYDFACPFVDSLEGITHALRSSEYHDR 420

Query: 421 NAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPTVQGIV 480
           NAQY+++  DMGLR+V +YEFSRLN+V+TLLSKRKLLWFVQ G VDGWDDPRFPTVQGIV
Sbjct: 421 NAQYFKVLEDMGLRQVQLYEFSRLNLVFTLLSKRKLLWFVQTGLVDGWDDPRFPTVQGIV 480

Query: 481 RRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEERRVLFTLEN 540
           RRGLK+EALIQFILEQ                    IIDPVCPRHTAV+ ERRVLFTL +
Sbjct: 481 RRGLKIEALIQFILEQGASKNLNLMEWDKLWSINKRIIDPVCPRHTAVVAERRVLFTLTD 540

Query: 541 GPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWGNAIVKGIE 600
           GP+ PF+R+IPKHKK+EGAGEK+TTFTK IW+++ DA  I V EE+TLMDWGNAIVK I 
Sbjct: 541 GPDEPFVRMIPKHKKFEGAGEKATTFTKSIWLEEADASAISVGEEVTLMDWGNAIVKEIT 600

Query: 601 KDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKKLEEGEDFL 649
           KD++G VT L G+L+L+GSVKTTKLK TWLPD NELV L+L E+DYLITKKKLE+ ++  
Sbjct: 601 KDEEGRVTALSGVLNLQGSVKTTKLKLTWLPDTNELVNLTLTEFDYLITKKKLEDDDEVA 660

BLAST of Clc01G11270 vs. ExPASy Swiss-Prot
Match: O13775 (Probable glutamate--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gus1 PE=1 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 4.7e-152
Identity = 278/566 (49.12%), Postives = 370/566 (65.37%), Query Frame = 0

Query: 103 KSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASGAKSQIVSTTRHLIRGKLEAN 162
           ++ ++ NL RW+     ++ D+ + V  T   +   KA     +  S+  +   G  +A 
Sbjct: 151 RTGQYFNLARWY-----KFMDSQNAVSVTM--EEFTKAVNISKKQKSSGPNYEIGLPDAI 210

Query: 163 IGKVRLRFAPEPSGYLHIGHSKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLK 222
            GKV  RF PEPSGYLHIGH+KAALLNQYFA +Y+G++I+RFDDTN +KE++EF + +L+
Sbjct: 211 DGKVVTRFPPEPSGYLHIGHAKAALLNQYFANKYHGKLIVRFDDTNPSKENSEFQDAILE 270

Query: 223 DIETLGIKYETVTYTSDYFPQLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSN 282
           D+  LGIK + VTYTSDY   + +   ++I+ G+AY DDT  E M+ ER EGI SK+R  
Sbjct: 271 DVALLGIKPDVVTYTSDYLDTIHQYCVDMIKSGQAYADDTDVETMRHERTEGIPSKHRDR 330

Query: 283 SAEENLRLWKEMVLGTEQGLLCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIY 342
             EE+L +  EM  G++ GL  C+R K+  ++PNK++RDPV YRCN +PHHR G+KYR Y
Sbjct: 331 PIEESLEILSEMDKGSDVGLKNCIRAKISYENPNKAMRDPVIYRCNLLPHHRTGTKYRAY 390

Query: 343 PTYDFACPFVDSIEGITHALRSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLS 402
           PTYDFACP VDS+EG+THALR++EY DRN  Y  +   M LRK+H++EFSR+N V TLLS
Sbjct: 391 PTYDFACPIVDSLEGVTHALRTTEYRDRNPLYQWMIKAMNLRKIHVWEFSRMNFVRTLLS 450

Query: 403 KRKLLWFVQNGKVDGWDDPRFPTVQGIVRRGLKVEALIQFILEQ---------------- 462
           KRKL   V +G V GWDDPRFPTV+G+ RRG+ +EAL Q+I+ Q                
Sbjct: 451 KRKLTEIVDHGLVWGWDDPRFPTVRGVRRRGMTIEALQQYIVSQGPSKNILTLDWTSFWA 510

Query: 463 ----IIDPVCPRHTAVIEERRVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWI 522
               IIDPV PRHTAV     V  T+ NGP  P+    P+HKK    G K + F   I I
Sbjct: 511 TNKKIIDPVAPRHTAVESGDVVKATIVNGPAAPYAEDRPRHKKNPELGNKKSIFANEILI 570

Query: 523 DQVDAQCIKVDEEITLMDWGNAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPD 582
           +Q DAQ  K DEE+TLMDWGNA V+ I +D  G VT L   LHL+G  K T+ K TWL D
Sbjct: 571 EQADAQSFKQDEEVTLMDWGNAYVREINRDASGKVTSLKLELHLDGDFKKTEKKVTWLAD 630

Query: 583 INELVTLSLMEYDYLITKKKLEEGEDFLDVLNPCTKKETVVVGDSNMRNIKRGDILQLER 642
             +   + L+++DYLITK KLEEGE++ D L P T+  + V  D  ++N+K+GDI+Q+ER
Sbjct: 631 TEDKTPVDLVDFDYLITKDKLEEGENYKDFLTPQTEFHSPVFADVGIKNLKKGDIIQVER 690

Query: 643 KGYFRCDVPYVRPSKPIVLFAIPDGR 649
           KGY+  DVP+       VLF IPDG+
Sbjct: 691 KGYYIVDVPF--DGTQAVLFNIPDGK 707

BLAST of Clc01G11270 vs. ExPASy Swiss-Prot
Match: P46655 (Glutamate--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GUS1 PE=1 SV=3)

HSP 1 Score: 526.9 bits (1356), Expect = 3.2e-148
Identity = 271/572 (47.38%), Postives = 370/572 (64.69%), Query Frame = 0

Query: 100 SLRKSKKHINLQRWFNSLLVE--YRDNLDDVLTTYAGKRGGKASGAKSQIVSTTRHLIRG 159
           S+ K+K  +N+ RW+  L ++  + +  D +  +    +     G K +       +   
Sbjct: 136 SIIKNKVDVNVSRWYTLLEMDPIFGEAHDFLSKSLLELKKSANVGKKKETHKANFEI--D 195

Query: 160 KLEANIGKVRLRFAPEPSGYLHIGHSKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFV 219
             +A +G+V  RF PEPSGYLHIGH+KAALLNQYFAQ Y G++IIRFDDTN +KE  EF 
Sbjct: 196 LPDAKMGEVVTRFPPEPSGYLHIGHAKAALLNQYFAQAYKGKLIIRFDDTNPSKEKEEFQ 255

Query: 220 ENLLKDIETLGIKYETVTYTSDYFPQLMEMAENLIRQGKAYIDDTPREQMQKERMEGIES 279
           +++L+D++ LGIK + +TY+SDYF ++ +    +I+ GKAY DDTP E+M++ERM+G+ S
Sbjct: 256 DSILEDLDLLGIKGDRITYSSDYFQEMYDYCVQMIKDGKAYCDDTPTEKMREERMDGVAS 315

Query: 280 KNRSNSAEENLRLW-KEMVLGTEQGLLCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIG 339
             R  S EENLR++ +EM  GTE+GL  C+R K+D +  NK+LRDPV YRCN  PHHR G
Sbjct: 316 ARRDRSVEENLRIFTEEMKNGTEEGLKNCVRAKIDYKALNKTLRDPVIYRCNLTPHHRTG 375

Query: 340 SKYRIYPTYDFACPFVDSIEGITHALRSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNM 399
           S ++IYPTYDF  P VD+IEG+THALR+ EY DRNAQY  +   + LRKVHI++F+R+N 
Sbjct: 376 STWKIYPTYDFCVPIVDAIEGVTHALRTIEYRDRNAQYDWMLQALRLRKVHIWDFARINF 435

Query: 400 VYTLLSKRKLLWFVQNGKVDGWDDPRFPTVQGIVRRGLKVEALIQFILEQ---------- 459
           V TLLSKRKL W V    V  WDDPRFPTV+G+ RRG+ VE L  F+L Q          
Sbjct: 436 VRTLLSKRKLQWMVDKDLVGNWDDPRFPTVRGVRRRGMTVEGLRNFVLSQGPSRNVINLE 495

Query: 460 ----------IIDPVCPRHTAVIEERRVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTF 519
                     +IDP+ PRHTA++   ++       P+ P I + PKHKK    GEK   +
Sbjct: 496 WNLIWAFNKKVIDPIAPRHTAIVNPVKIHLEGSEAPQEPKIEMKPKHKKNPAVGEKKVIY 555

Query: 520 TKRIWIDQVDAQCIKVDEEITLMDWGNAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLK 579
            K I +D+ DA  I VDEE+TLMDWGN I+   +K+ DG    ++  L+LEG  K TK K
Sbjct: 556 YKDIVVDKDDADVINVDEEVTLMDWGNVII--TKKNDDG---SMVAKLNLEGDFKKTKHK 615

Query: 580 ATWLPDINELVTLSLMEYDYLITKKKLEEGEDFLDVLNPCTKKETVVVGDSNMRNIKRGD 639
            TWL D  ++V + L+++D+LITK +LEE E F D L P T+  T  + D N++++K GD
Sbjct: 616 LTWLADTKDVVPVDLVDFDHLITKDRLEEDESFEDFLTPQTEFHTDAIADLNVKDMKIGD 675

Query: 640 ILQLERKGYFRCDVPYVRPSKPIVLFAIPDGR 649
           I+Q ERKGY+R D    +  KP V F IPDG+
Sbjct: 676 IIQFERKGYYRLDA-LPKDGKPYVFFTIPDGK 699

BLAST of Clc01G11270 vs. ExPASy Swiss-Prot
Match: Q54KB8 (Probable glutamate--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=gluS PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.2e-139
Identity = 282/672 (41.96%), Postives = 392/672 (58.33%), Query Frame = 0

Query: 50  LYGTSVLLRYIGRVTNIPNFYGPNAYESSQIDEWL-------------------EYAPIL 109
           L G+ V+ +Y+ R T   + YG NA  +S+IDE++                   EY  + 
Sbjct: 57  LKGSYVIAKYLARTTPSLSLYGENALSASKIDEFIDKFAHLKSEKFNEFLKEMNEYLTLR 116

Query: 110 SSGTG-----------QRWESLRKSKKHIN--------LQRWFNSL-----LVEYRDNLD 169
           +   G            R + +++ ++ IN        L RW+  L      VE  +  +
Sbjct: 117 AFLIGFNLTLADIVLFARIKMVKEIQEEINKLGKTIPHLNRWYGYLSQLESFVEADNAFN 176

Query: 170 DVLTTYA-GKRGGKASGAKSQIVSTTRHLIR--GKLEA-NI-----GKVRLRFAPEPSGY 229
               T A GK G + + A ++ V+  +  +   G  EA N+     GKV  RF PEPSGY
Sbjct: 177 GKKETKASGKAGAEGTAATTEKVAPQKGAMGWVGNFEALNLPGLVEGKVVTRFPPEPSGY 236

Query: 230 LHIGHSKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYT 289
           +HIGH KAA++N Y+A++YNG++IIRFDDTN +KE  E+VEN++KDI  LGIKYE +T+T
Sbjct: 237 MHIGHCKAAIINNYYAEKYNGKIIIRFDDTNPSKEKEEYVENIIKDINNLGIKYEKITHT 296

Query: 290 SDYFPQLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLG 349
           SDYF  + + A  +I++G AY DDTP+ +M +ER   IES +R+NS E+NL ++ EM   
Sbjct: 297 SDYFDLIHDYAIQMIKEGIAYCDDTPQVKMSEERDNAIESVHRNNSVEKNLEMFDEMKKA 356

Query: 350 TEQGLLCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEG 409
           TEQG+ C LR KLDM   +K+ RDP  YRCN  PHHR G KY++YP YDFACP VDS+EG
Sbjct: 357 TEQGVKCVLRAKLDMAHIDKAFRDPAIYRCNSTPHHRTGDKYKVYPLYDFACPIVDSVEG 416

Query: 410 ITHALRSSEYHDRNAQYYRIQGDMGL-RKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVD 469
           ITHALRS+EY+++   Y      + L  K +I ++SRL+    LLSKRKL  FV  G V 
Sbjct: 417 ITHALRSNEYNNKRNLYNHYLEILHLENKPYISDYSRLSFFNVLLSKRKLQHFVDTGLVS 476

Query: 470 GWDDPRFPTVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHT 529
           GW DPR PT+QGI RRGL V AL +FIL Q                    +++P CPR+T
Sbjct: 477 GWTDPRLPTLQGITRRGLTVAALKEFILSQGASAANTTLDLGKLFVGNKAVLEPTCPRYT 536

Query: 530 AVIEERRVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEI 589
           A+ +   V FTL NGP +P ++   K+ K    G K  TF+  + ++  D   IK  EE+
Sbjct: 537 AIAKATAVKFTLSNGPTLPEVKDCLKYAKDPSMGTKKVTFSNNLLLEGDDCNQIKEGEEV 596

Query: 590 TLMDWGNAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWL-PDINELVTLSLMEYD 648
           TLM+WGNAIV+ ++++++G V  + G LHLEG VK T  K +WL  D  + VT+ L +YD
Sbjct: 597 TLMNWGNAIVETLQRNENGDVVSMTGKLHLEGDVKKTDKKLSWLSSDCADTVTVVLQDYD 656

BLAST of Clc01G11270 vs. ExPASy Swiss-Prot
Match: Q8CGC7 (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4)

HSP 1 Score: 495.0 bits (1273), Expect = 1.3e-138
Identity = 268/663 (40.42%), Postives = 384/663 (57.92%), Query Frame = 0

Query: 56  LLRYIGRVTNIPNFYGPNAYESSQIDEWLEY-APILSS---------------------- 115
           +LRY+ R+      YG N  E ++ID WLE+ A  LSS                      
Sbjct: 54  ILRYLARIATTSGLYGTNLMEHTEIDHWLEFSATKLSSCDRLTSAINELNHCLSLRTYLV 113

Query: 116 ----------------GTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKR 175
                           G+    E L+++K  ++++RWF  L  +            +G R
Sbjct: 114 GNSLTLADLCVWATLKGSAAWQEHLKQNKTLVHVKRWFGFLEAQQAFRSVGTKWDVSGNR 173

Query: 176 GGKASGAKSQIVSTTRHLIRGKL----EANIGKVRLRFAPEPSGYLHIGHSKAALLNQYF 235
              A   K  +         GK      A +GKV +RF PE SGYLHIGH+KAALLNQ++
Sbjct: 174 ATVAPDKKQDV---------GKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHY 233

Query: 236 AQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLMEMAENLI 295
              + G++I+RFDDTN  KE  +F + +L+D+  L IK +  TYTSD+F  +M+ AE LI
Sbjct: 234 QVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLI 293

Query: 296 RQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLCCLRGKLDM 355
           ++GKAY+DDTP EQM+ ER +  ESK+R NS E+NL++W+EM  G++ G  CCLR K+DM
Sbjct: 294 QEGKAYVDDTPAEQMKAEREQRTESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDM 353

Query: 356 QDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRSSEYHDRNA 415
              N  +RDP  YRC   PH R G+KY +YPTYDFACP VDSIEG+THALR++EYHDR+ 
Sbjct: 354 SSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDE 413

Query: 416 QYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPTVQGIVRR 475
           Q+Y I   +G+RK +I+E+SRLN+  T+LSKRKL WFV  G VDGWDDPRFPTV+G++RR
Sbjct: 414 QFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRR 473

Query: 476 GLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEERRVLFTLENGP 535
           G+ VE L QFI  Q                    +IDPV PR+ A++++  V   + +  
Sbjct: 474 GMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVVPVNVLDAQ 533

Query: 536 EIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWGNAIVKGIEKD 595
           E   ++ + +H K    G K   ++ +++I+  DA+     E +T ++WGN  +  I K+
Sbjct: 534 E--EMKEVARHPKNPDVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNINITKIHKN 593

Query: 596 QDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLS--LMEYDYLITKKKLEEGEDFL 648
            DG +T L   L+LE        K TWL +    +++    + Y++LITK  L + EDF 
Sbjct: 594 ADGKITSLDAKLNLENKDYKKTTKITWLAESTHALSIPAVCVTYEHLITKPVLGKDEDFK 653

BLAST of Clc01G11270 vs. ExPASy TrEMBL
Match: A0A6J1HA76 (Glutamyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111461971 PE=3 SV=1)

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 604/732 (82.51%), Postives = 624/732 (85.25%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKLLSFPAD+PP  VIVAAK SGITF  DSSLASGSAPTFLFTNG KL+GTSVLLRYI
Sbjct: 1   MDIKLLSFPADSPPLSVIVAAKFSGITFPADSSLASGSAPTFLFTNGLKLHGTSVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILS----------------------------- 120
           GRVTNIPNFYG NAYESSQIDEWLEYAPILS                             
Sbjct: 61  GRVTNIPNFYGENAYESSQIDEWLEYAPILSTGSAFENACSYVDKYLERRTFLVSHSLSL 120

Query: 121 ---------SGTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                    +GTG RWESLRKSKK++NLQRWFNSLLVEY D LDDVLTTY GKRGGK+SG
Sbjct: 121 ADVAIWSGLAGTGPRWESLRKSKKYLNLQRWFNSLLVEYSDGLDDVLTTYVGKRGGKSSG 180

Query: 181 AK---SQIVSTTRHLI------RGKL-----------EANIGKVRLRFAPEPSGYLHIGH 240
           AK    Q ++   + +      +GK             A IGKVRLRFAPEPSGYLHIGH
Sbjct: 181 AKLKDQQGLTQKPNSVNQDTSDKGKSGSKSTFEVDLPNAEIGKVRLRFAPEPSGYLHIGH 240

Query: 241 SKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300
           SKAALLNQYFAQRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYFP
Sbjct: 241 SKAALLNQYFAQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300

Query: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGL 360
           QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNS EENLRLWKEM+LGTE+GL
Sbjct: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSPEENLRLWKEMILGTEKGL 360

Query: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHAL 420
           LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKY+IYPTYDFACPFVDSIEGITHAL
Sbjct: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYKIYPTYDFACPFVDSIEGITHAL 420

Query: 421 RSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480
           RSSEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR
Sbjct: 421 RSSEYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480

Query: 481 FPTVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEER 540
           FPTVQGIVRRGLKVEALIQFILEQ                    IIDPVCPRHTAVIEER
Sbjct: 481 FPTVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVIEER 540

Query: 541 RVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWG 600
           RVLFTL+NGPE PF+RVIPKHKKYEGAGEKS+TFTKRIWIDQVDAQCIKVDEEITLMDWG
Sbjct: 541 RVLFTLDNGPEKPFVRVIPKHKKYEGAGEKSSTFTKRIWIDQVDAQCIKVDEEITLMDWG 600

Query: 601 NAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 655
           NAI+KGIEKD DGFVT+L GILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK
Sbjct: 601 NAILKGIEKDGDGFVTRLSGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 660

BLAST of Clc01G11270 vs. ExPASy TrEMBL
Match: A0A6J1I0W4 (Glutamyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468000 PE=3 SV=1)

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 599/732 (81.83%), Postives = 623/732 (85.11%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKLLSFPAD+PP  VIVAAK + I F  DSSLASGSAPTFLFTNGSKL+GTSVLLRYI
Sbjct: 1   MDIKLLSFPADSPPLSVIVAAKFASIPFPADSSLASGSAPTFLFTNGSKLHGTSVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILS----------------------------- 120
           GRVTNIPNFYG NAYESSQIDEWLEYAPILS                             
Sbjct: 61  GRVTNIPNFYGENAYESSQIDEWLEYAPILSTGSAFENACSYVDKYLKRRTFLVSHSLSL 120

Query: 121 ---------SGTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                    +GTG RWESLRKSKK++NLQRWFNSLLVEY D+LDDVLTTY GKRGGK+SG
Sbjct: 121 ADVAIWSGLAGTGPRWESLRKSKKYLNLQRWFNSLLVEYSDDLDDVLTTYVGKRGGKSSG 180

Query: 181 AK---SQIVSTTRHLI------RGKL-----------EANIGKVRLRFAPEPSGYLHIGH 240
           AK    Q ++   + +      +GK             A IG+VRLRFAPEPSGYLHIGH
Sbjct: 181 AKLKDQQGLTQKPNSVNQDTSDKGKSGSKSTFEVDLPNAEIGEVRLRFAPEPSGYLHIGH 240

Query: 241 SKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFP 300
           SKAALLNQYFAQRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYF 
Sbjct: 241 SKAALLNQYFAQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFL 300

Query: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGL 360
           QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNS EENLRLWKEM+LGTE+GL
Sbjct: 301 QLMEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSPEENLRLWKEMILGTEKGL 360

Query: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHAL 420
           LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSK++IYPTYDFACPFVDSIEGITHAL
Sbjct: 361 LCCLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKFKIYPTYDFACPFVDSIEGITHAL 420

Query: 421 RSSEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480
           RSSEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR
Sbjct: 421 RSSEYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPR 480

Query: 481 FPTVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEER 540
           FPTVQGIVRRGLKVEALIQFILEQ                    IIDPVCPRHTAVIEER
Sbjct: 481 FPTVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVIEER 540

Query: 541 RVLFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWG 600
           RVLFTL+NGPE PFIRV+PKHKKYEGAGEKS+TFTKRIWIDQVDAQCIKVDEEITLMDWG
Sbjct: 541 RVLFTLDNGPEKPFIRVLPKHKKYEGAGEKSSTFTKRIWIDQVDAQCIKVDEEITLMDWG 600

Query: 601 NAIVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 655
           NAIVKGIEKD DGFVT+L GILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK
Sbjct: 601 NAIVKGIEKDGDGFVTRLSGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKK 660

BLAST of Clc01G11270 vs. ExPASy TrEMBL
Match: A0A1S3BPD2 (Glutamyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103492081 PE=3 SV=1)

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 596/730 (81.64%), Postives = 615/730 (84.25%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKL SFPAD  PF VIVAAKL+GI F  DSSL S S PTFLFT+GSKL+G SVLLRYI
Sbjct: 1   MDIKLFSFPADNTPFSVIVAAKLAGIAFPSDSSLPSASPPTFLFTDGSKLHGASVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILSS---------------------------- 120
           GRVTNIPNFYG NAYESS+IDEWLEYAPILSS                            
Sbjct: 61  GRVTNIPNFYGQNAYESSRIDEWLEYAPILSSGSAFENACSYVDKYLERCTFLVGHSLSL 120

Query: 121 ----------GTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                     GTGQRWESLRKSKK++NLQRWFNSLLVEY D LDDVLT + GKRGGK+SG
Sbjct: 121 ADVAVWSGLAGTGQRWESLRKSKKYLNLQRWFNSLLVEYSDELDDVLTAFVGKRGGKSSG 180

Query: 181 AK---SQIVSTT----RHLIRGKL-----------EANIGKVRLRFAPEPSGYLHIGHSK 240
            K    Q + T         +GK                GKVRLRFAPEPSGYLHIGHSK
Sbjct: 181 PKLKDQQGLKTNSVNQEASDKGKAGSKSTFEVDLPNVEFGKVRLRFAPEPSGYLHIGHSK 240

Query: 241 AALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQL 300
           AALLNQYF+QRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYFPQL
Sbjct: 241 AALLNQYFSQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQL 300

Query: 301 MEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLC 360
           MEMAENLIRQGKAYIDDTPREQMQKERMEGIESK RSNS EENLRLWKEM+LGTEQGLLC
Sbjct: 301 MEMAENLIRQGKAYIDDTPREQMQKERMEGIESKRRSNSPEENLRLWKEMILGTEQGLLC 360

Query: 361 CLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRS 420
           CLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKY+IYPTYDFACPFVDSIEGITHALRS
Sbjct: 361 CLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYKIYPTYDFACPFVDSIEGITHALRS 420

Query: 421 SEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFP 480
           SEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFP
Sbjct: 421 SEYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFP 480

Query: 481 TVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEERRV 540
           TVQGIVRRGLKVEALIQFILEQ                    IIDPVCPRHTAVIEERRV
Sbjct: 481 TVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVIEERRV 540

Query: 541 LFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWGNA 600
           LFTL NGPEIP++R+IPKHKKYEGAGEKSTTFTKRIWIDQ+DAQCIKVDEEITLMDWGNA
Sbjct: 541 LFTLGNGPEIPYVRIIPKHKKYEGAGEKSTTFTKRIWIDQIDAQCIKVDEEITLMDWGNA 600

Query: 601 IVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKKLE 655
           IVKGI+KDQDGFVTQL GILHLEGSVKTTKLK TWLPDI ELVTLSLMEYDYLITKKKLE
Sbjct: 601 IVKGIQKDQDGFVTQLSGILHLEGSVKTTKLKITWLPDIKELVTLSLMEYDYLITKKKLE 660

BLAST of Clc01G11270 vs. ExPASy TrEMBL
Match: A0A5A7U6S0 (Glutamyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004790 PE=3 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 595/730 (81.51%), Postives = 615/730 (84.25%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKL SFPAD  PF VIVAAKL+GI F  DSSL S S PTFLFT+GSKL+G SVLLRYI
Sbjct: 1   MDIKLFSFPADNTPFSVIVAAKLAGIAFPSDSSLPSASPPTFLFTDGSKLHGASVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILSS---------------------------- 120
           GRVTNIPNFYG NAYESS+IDEWLEYAPILSS                            
Sbjct: 61  GRVTNIPNFYGQNAYESSRIDEWLEYAPILSSGSAFENACSYVDKYLERCTFLVGHSLSL 120

Query: 121 ----------GTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                     GTGQRWESLRKSKK++NLQRWFNSLLVEY D LDDVLT + GKRGGK+SG
Sbjct: 121 ADVAVWSGLAGTGQRWESLRKSKKYLNLQRWFNSLLVEYSDELDDVLTAFVGKRGGKSSG 180

Query: 181 AK---SQIVSTT----RHLIRGKL-----------EANIGKVRLRFAPEPSGYLHIGHSK 240
            K    Q + T         +GK                GKVRLRFAPEPSGYLHIGHSK
Sbjct: 181 PKLKDQQGLKTNSVNQEASDKGKAGSKSTFEVDLPNVEFGKVRLRFAPEPSGYLHIGHSK 240

Query: 241 AALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQL 300
           AALLNQYF+QRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYFPQL
Sbjct: 241 AALLNQYFSQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQL 300

Query: 301 MEMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLC 360
           MEMAENLIRQGKAYIDDTPREQMQKERMEGIESK RSNS EENLRLWKEM+LGTEQGLLC
Sbjct: 301 MEMAENLIRQGKAYIDDTPREQMQKERMEGIESKRRSNSPEENLRLWKEMILGTEQGLLC 360

Query: 361 CLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRS 420
           CLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKY+IYPTYDFACPFVDSIEGITHALRS
Sbjct: 361 CLRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYKIYPTYDFACPFVDSIEGITHALRS 420

Query: 421 SEYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFP 480
           SEYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFP
Sbjct: 421 SEYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFP 480

Query: 481 TVQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEERRV 540
           TVQGIVRRGLKVEALIQFILEQ                    IIDPVCPRHTAVIEERRV
Sbjct: 481 TVQGIVRRGLKVEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVIEERRV 540

Query: 541 LFTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWGNA 600
           LFTL NGPEIP++R+IPKHKKYEGAGEKSTTFTKRIWIDQ+DAQCIKVDEEITLMDWGNA
Sbjct: 541 LFTLGNGPEIPYVRIIPKHKKYEGAGEKSTTFTKRIWIDQIDAQCIKVDEEITLMDWGNA 600

Query: 601 IVKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKKLE 655
           IVKGI+KDQDGFVTQL GILHLEGSVKTTKLK TWLPDI ELVTLSLMEYDYLITKKKLE
Sbjct: 601 IVKGIQKDQDGFVTQLSGILHLEGSVKTTKLKITWLPDIKELVTLSLMEYDYLITKKKLE 660

BLAST of Clc01G11270 vs. ExPASy TrEMBL
Match: A0A0A0LYW4 (Glutamyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_1G561380 PE=3 SV=1)

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 590/729 (80.93%), Postives = 611/729 (83.81%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MDIKLLSFPAD  PF VIVAAKL+GITF  DSSL S S PTF FT+G KL+G SVLLRYI
Sbjct: 1   MDIKLLSFPADNTPFSVIVAAKLAGITFPSDSSLPSASPPTFFFTDGLKLHGASVLLRYI 60

Query: 61  GRVTNIPNFYGPNAYESSQIDEWLEYAPILSS---------------------------- 120
           GRVTNIPNFYG NA+ESSQIDEWLEYAPILSS                            
Sbjct: 61  GRVTNIPNFYGQNAFESSQIDEWLEYAPILSSGSAFENACSYVDKYLERRTFLVGHSLSL 120

Query: 121 ----------GTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKASG 180
                     GTGQRWESLRKSKK++NLQRWFNSLLVEY D LDDVLT + GKRGGK+SG
Sbjct: 121 ADVAIWSGLAGTGQRWESLRKSKKYLNLQRWFNSLLVEYSDELDDVLTAFVGKRGGKSSG 180

Query: 181 AKSQIVSTTRHLI------RGKL-----------EANIGKVRLRFAPEPSGYLHIGHSKA 240
            K +      + +      +GK                GKVRLRFAPEPSGYLHIGHSKA
Sbjct: 181 PKLKEQGLKTNSVNQDASDKGKAGSKSTFEVDLPNVEFGKVRLRFAPEPSGYLHIGHSKA 240

Query: 241 ALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLM 300
           ALLNQYFAQRYNGEVIIRFDDTN AKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLM
Sbjct: 241 ALLNQYFAQRYNGEVIIRFDDTNPAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLM 300

Query: 301 EMAENLIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLCC 360
           EMAENLIRQGKAYIDDTPREQMQKERMEGIESK+RSNS  ENLRLWKEM+LGTEQGLLCC
Sbjct: 301 EMAENLIRQGKAYIDDTPREQMQKERMEGIESKSRSNSPGENLRLWKEMILGTEQGLLCC 360

Query: 361 LRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRSS 420
           LRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKY+IYPTYDFACPFVDSIEGITHALRSS
Sbjct: 361 LRGKLDMQDPNKSLRDPVYYRCNPIPHHRIGSKYKIYPTYDFACPFVDSIEGITHALRSS 420

Query: 421 EYHDRNAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPT 480
           EYHDRNAQYYRIQ DMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPT
Sbjct: 421 EYHDRNAQYYRIQEDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPT 480

Query: 481 VQGIVRRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEERRVL 540
           VQGIVRRGLK+EALIQFILEQ                    IIDPVCPRHTAVIEERRVL
Sbjct: 481 VQGIVRRGLKIEALIQFILEQGASKNLNLMEWDKLWTINKKIIDPVCPRHTAVIEERRVL 540

Query: 541 FTLENGPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWGNAI 600
           FTL NGPEIPF+R+IPKHKKYEGAGEKSTTFTKRIWIDQ DAQCI+VDEEITLMDWGNAI
Sbjct: 541 FTLGNGPEIPFVRIIPKHKKYEGAGEKSTTFTKRIWIDQSDAQCIEVDEEITLMDWGNAI 600

Query: 601 VKGIEKDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKKLEE 655
           VKGIEKDQDGFV QL GILHLEGSVKTTKLK TWLP+I ELVTLSLMEYDYLITKKKLEE
Sbjct: 601 VKGIEKDQDGFVKQLSGILHLEGSVKTTKLKITWLPEIKELVTLSLMEYDYLITKKKLEE 660

BLAST of Clc01G11270 vs. TAIR 10
Match: AT5G26710.1 (Glutamyl/glutaminyl-tRNA synthetase, class Ic )

HSP 1 Score: 935.6 bits (2417), Expect = 2.1e-272
Identity = 462/718 (64.35%), Postives = 554/718 (77.16%), Query Frame = 0

Query: 1   MDIKLLSFPADTPPFFVIVAAKLSGITFSEDSSLASGSAPTFLFTNGSKLYGTSVLLRYI 60
           MD   LSFP ++PP  VIVA  LS    + DSS A+ + P+F+F++G KL G +VLLRY+
Sbjct: 1   MDGMKLSFPPESPPLSVIVALSLSASPVTIDSSAAATTVPSFVFSDGRKLNGATVLLRYV 60

Query: 61  GR-VTNIPNFYGPNAYESSQIDEWLEYAPILSS--------------------------- 120
           GR    +P+FYG NA++SSQIDEW++YA + SS                           
Sbjct: 61  GRSAKKLPDFYGNNAFDSSQIDEWVDYASVFSSGSEFENACGRVDKYLESSTFLVGHSLS 120

Query: 121 -----------GTGQRWESLRKSKKHINLQRWFNSLLVEYRDNLDDVLTTYAGKRGGKAS 180
                      GTGQRWESLRKSKK+ +L RWFNS+L EY + L+ VL TY  K  GK  
Sbjct: 121 IADVAIWSALAGTGQRWESLRKSKKYQSLVRWFNSILDEYSEVLNKVLATYVKKGSGKPV 180

Query: 181 GAKSQIVSTTRHLIRGK-----------LEANIGKVRLRFAPEPSGYLHIGHSKAALLNQ 240
            A       ++  ++G             EA IGKV+LRFAPEPSGYLHIGH+KAALLN+
Sbjct: 181 AAPKS--KDSQQAVKGDGQDKGKPEVDLPEAEIGKVKLRFAPEPSGYLHIGHAKAALLNK 240

Query: 241 YFAQRYNGEVIIRFDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLMEMAEN 300
           YFA+RY GEVI+RFDDTN AKESNEFV+NL+KDI TLGIKYE VTYTSDYFP+LM+MAE 
Sbjct: 241 YFAERYQGEVIVRFDDTNPAKESNEFVDNLVKDIGTLGIKYEKVTYTSDYFPELMDMAEK 300

Query: 301 LIRQGKAYIDDTPREQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLCCLRGKL 360
           L+R+GKAY+DDTPREQMQKERM+GI+SK R++S EENL+LWKEM+ G+E+GL CC+RGK 
Sbjct: 301 LMREGKAYVDDTPREQMQKERMDGIDSKCRNHSVEENLKLWKEMIAGSERGLQCCVRGKF 360

Query: 361 DMQDPNKSLRDPVYYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRSSEYHDR 420
           +MQDPNK++RDPVYYRCNP+ HHRIG KY+IYPTYDFACPFVDS+EGITHALRSSEYHDR
Sbjct: 361 NMQDPNKAMRDPVYYRCNPMSHHRIGDKYKIYPTYDFACPFVDSLEGITHALRSSEYHDR 420

Query: 421 NAQYYRIQGDMGLRKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPTVQGIV 480
           NAQY+++  DMGLR+V +YEFSRLN+V+TLLSKRKLLWFVQ G VDGWDDPRFPTVQGIV
Sbjct: 421 NAQYFKVLEDMGLRQVQLYEFSRLNLVFTLLSKRKLLWFVQTGLVDGWDDPRFPTVQGIV 480

Query: 481 RRGLKVEALIQFILEQ--------------------IIDPVCPRHTAVIEERRVLFTLEN 540
           RRGLK+EALIQFILEQ                    IIDPVCPRHTAV+ ERRVLFTL +
Sbjct: 481 RRGLKIEALIQFILEQGASKNLNLMEWDKLWSINKRIIDPVCPRHTAVVAERRVLFTLTD 540

Query: 541 GPEIPFIRVIPKHKKYEGAGEKSTTFTKRIWIDQVDAQCIKVDEEITLMDWGNAIVKGIE 600
           GP+ PF+R+IPKHKK+EGAGEK+TTFTK IW+++ DA  I V EE+TLMDWGNAIVK I 
Sbjct: 541 GPDEPFVRMIPKHKKFEGAGEKATTFTKSIWLEEADASAISVGEEVTLMDWGNAIVKEIT 600

Query: 601 KDQDGFVTQLLGILHLEGSVKTTKLKATWLPDINELVTLSLMEYDYLITKKKLEEGEDFL 649
           KD++G VT L G+L+L+GSVKTTKLK TWLPD NELV L+L E+DYLITKKKLE+ ++  
Sbjct: 601 KDEEGRVTALSGVLNLQGSVKTTKLKLTWLPDTNELVNLTLTEFDYLITKKKLEDDDEVA 660

BLAST of Clc01G11270 vs. TAIR 10
Match: AT1G25350.1 (glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, putative )

HSP 1 Score: 227.3 bits (578), Expect = 3.7e-59
Identity = 118/295 (40.00%), Postives = 181/295 (61.36%), Query Frame = 0

Query: 149 STTRHLIRGKLEANIGKVRLRFAPEPSGYLHIGHSKAALLNQYFAQRYNGEVIIRFDDTN 208
           S T+ ++   L+   GKV  RF PEP+GYLHIGH+KA  ++   A+   G   +R+DDTN
Sbjct: 254 SNTKEVLDKHLKVTGGKVYTRFPPEPNGYLHIGHAKAMFVDFGLAKERGGCCYLRYDDTN 313

Query: 209 LAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLMEMAENLIRQGKAYIDDTPREQMQ 268
              E  E++ ++ + ++ +G +   +TYTSDYF +L ++A  LIR+G AY+D    ++++
Sbjct: 314 PEAEKEEYINHIEEIVKWMGWEPFKITYTSDYFQELYDLAVELIRRGHAYVDHQTADEIK 373

Query: 269 KERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLCCLRGKLDMQDPNKSLRDPVYYRCN 328
           + R + + S  R    EE+L+L+ EM  G  +     LR K DMQ  N ++ D + YR  
Sbjct: 374 EYREKKMNSPWRDRPIEESLKLFDEMRRGIIEEGKATLRMKQDMQSDNFNMYDLIAYRIK 433

Query: 329 PIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRSSEYHDRNAQYYRIQGDMGLRKVHI 388
             PH + G K+ IYP+YD+A   VDS+E ITH+L + E+  R A YY +   + L   ++
Sbjct: 434 FAPHPKAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASYYWLLHSLSLYMPYV 493

Query: 389 YEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPTVQGIVRRGLKVEALIQFI 444
           +E+SRLN+  T++SKRKL + V N  VDGWDDPR  T+ G+ RRG+   A+  F+
Sbjct: 494 WEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRGVTSTAINAFV 548

BLAST of Clc01G11270 vs. TAIR 10
Match: AT1G25350.2 (glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, putative )

HSP 1 Score: 203.0 bits (515), Expect = 7.4e-52
Identity = 112/300 (37.33%), Postives = 177/300 (59.00%), Query Frame = 0

Query: 149 STTRHLIRGKLEANIGKVRLRFAPEPSG---YLHIG--HSKAALLNQYFAQRYNGEVIIR 208
           S T+ ++   L+   GKV  RF PEP+G   Y+     + +A  ++   A+   G   +R
Sbjct: 254 SNTKEVLDKHLKVTGGKVYTRFPPEPNGLWFYVRFQCLYFQAMFVDFGLAKERGGCCYLR 313

Query: 209 FDDTNLAKESNEFVENLLKDIETLGIKYETVTYTSDYFPQLMEMAENLIRQGKAYIDDTP 268
           +DDTN   E  E++ ++ + ++ +G +   +TYTSDYF +L ++A  LIR+G AY+D   
Sbjct: 314 YDDTNPEAEKEEYINHIEEIVKWMGWEPFKITYTSDYFQELYDLAVELIRRGHAYVDHQT 373

Query: 269 REQMQKERMEGIESKNRSNSAEENLRLWKEMVLGTEQGLLCCLRGKLDMQDPNKSLRDPV 328
            +++++ R + + S  R    EE+L+L+ EM  G  +     LR K DMQ  N ++ D +
Sbjct: 374 ADEIKEYREKKMNSPWRDRPIEESLKLFDEMRRGIIEEGKATLRMKQDMQSDNFNMYDLI 433

Query: 329 YYRCNPIPHHRIGSKYRIYPTYDFACPFVDSIEGITHALRSSEYHDRNAQYYRIQGDMGL 388
            YR    PH + G K+ IYP+YD+A   VDS+E ITH+L + E+  R A YY +   + L
Sbjct: 434 AYRIKFAPHPKAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASYYWLLHSLSL 493

Query: 389 RKVHIYEFSRLNMVYTLLSKRKLLWFVQNGKVDGWDDPRFPTVQGIVRRGLKVEALIQFI 444
              +++E+SRLN+  T++SKRKL + V N  VDGWDDPR  T+ G+ RRG+   A+  F+
Sbjct: 494 YMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRGVTSTAINAFV 553

BLAST of Clc01G11270 vs. TAIR 10
Match: AT5G64050.1 (glutamate tRNA synthetase )

HSP 1 Score: 66.6 bits (161), Expect = 8.3e-11
Identity = 36/106 (33.96%), Postives = 58/106 (54.72%), Query Frame = 0

Query: 162 NIGKVRLRFAPEPSGYLHIGHSKAALLNQYFAQRYNGEVIIRFDDTNLAKESNEFVENLL 221
           N G VR+RFAP P+G LH+G ++ AL N  FA+   G+ ++R +DT+L + + E    +L
Sbjct: 50  NGGSVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTRESEAAVL 109

Query: 222 KDIETLGIKY-ETVTYTSDYFPQ--------LMEMAENLIRQGKAY 259
           +D+  LG+ + E    + D+ P           + AE L+  G  Y
Sbjct: 110 QDLSWLGLDWDEGPGVSGDFGPYRQSERNALYKQYAEKLLESGHVY 155

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895456.10.0e+0082.86glutamate--tRNA ligase, cytoplasmic-like [Benincasa hispida] >XP_038895457.1 glu... [more]
XP_022961381.10.0e+0082.51glutamate--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022961382.1 gl... [more]
KAG6590522.10.0e+0082.24Glutamate--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_023521072.10.0e+0082.24glutamate--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] >XP_023521... [more]
XP_038878933.10.0e+0081.97glutamate--tRNA ligase, cytoplasmic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O824623.0e-27164.35Glutamate--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g26710... [more]
O137754.7e-15249.12Probable glutamate--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strai... [more]
P466553.2e-14847.38Glutamate--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204... [more]
Q54KB81.2e-13941.96Probable glutamate--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=4468... [more]
Q8CGC71.3e-13840.42Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1HA760.0e+0082.51Glutamyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111461971 PE=3 SV=1[more]
A0A6J1I0W40.0e+0081.83Glutamyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468000 PE=3 SV=1[more]
A0A1S3BPD20.0e+0081.64Glutamyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103492081 PE=3 SV=1[more]
A0A5A7U6S00.0e+0081.51Glutamyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0LYW40.0e+0080.93Glutamyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_1G561380 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G26710.12.1e-27264.35Glutamyl/glutaminyl-tRNA synthetase, class Ic [more]
AT1G25350.13.7e-5940.00glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, ... [more]
AT1G25350.27.4e-5237.33glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, ... [more]
AT5G64050.18.3e-1133.96glutamate tRNA synthetase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000924Glutamyl/glutaminyl-tRNA synthetasePRINTSPR00987TRNASYNTHGLUcoord: 343..353
score: 51.82
coord: 183..194
score: 42.5
coord: 359..367
score: 47.22
coord: 169..181
score: 71.73
coord: 198..211
score: 43.57
IPR020059Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domainPFAMPF03950tRNA-synt_1c_Ccoord: 452..629
e-value: 2.6E-33
score: 115.3
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 138..452
e-value: 1.8E-112
score: 377.7
IPR020058Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domainPFAMPF00749tRNA-synt_1ccoord: 165..445
e-value: 4.0E-99
score: 331.5
IPR020056Ribosomal protein L25/Gln-tRNA synthetase, N-terminalGENE3D2.40.240.10Ribosomal Protein L25; Chain Pcoord: 561..642
e-value: 2.3E-9
score: 39.3
NoneNo IPR availableGENE3D1.20.1050.130coord: 3..96
e-value: 6.1E-11
score: 44.3
NoneNo IPR availablePANTHERPTHR43097:SF12OS01G0271200 PROTEINcoord: 6..93
coord: 92..652
NoneNo IPR availablePANTHERPTHR43097GLUTAMINE-TRNA LIGASEcoord: 6..93
coord: 92..652
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 165..469
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 172..183
IPR011035Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamilySUPERFAMILY50715Ribosomal protein L25-likecoord: 452..649

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc01G11270.2Clc01G11270.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006424 glutamyl-tRNA aminoacylation
biological_process GO:0006412 translation
biological_process GO:0043039 tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005829 cytosol
cellular_component GO:0017102 methionyl glutamyl tRNA synthetase complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004818 glutamate-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding