Cla97C11G215200 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C11G215200
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	RING-type E3 ubiquitin transferase
Location	Cla97Chr11: 11342625 .. 11343026 (+)
RNA-Seq Expression	Cla97C11G215200
Synteny	Cla97C11G215200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCCCACTCTCAAACCCCCCATCCATGAATCCAATCCTCTACCTCATTTCTACTGCCAACACTTCCCCTTCTTCCTTAGTCTCCTCCATCAGACGTCTCCGCTCTATCTCCGCCGACTCTAAAACCAACCGCTGTTTTGTCGACACCGACTCCTCTGCTTCCTATGGAGCTCTCTCCTCTCTCCATAACCTCCGTCTCCCCGATTCAACATTCAAACGCTTGGCTACCCACCCTGAGTTTCTCTATTCATTGACGAATTTCATGAAGTTACAGGGTACTCGTGAATCGTCCAGAGCATACGTGGTGTTGATTTTGAAATCGATCTTCGAAGTCACTGATCCAATTCAACTAAGATTCTTAAATCCGATTAATTTGTGCAAATTGTTGAGATTTTGA

mRNA sequence

ATGGCTTCCCCACTCTCAAACCCCCCATCCATGAATCCAATCCTCTACCTCATTTCTACTGCCAACACTTCCCCTTCTTCCTTAGTCTCCTCCATCAGACGTCTCCGCTCTATCTCCGCCGACTCTAAAACCAACCGCTGTTTTGTCGACACCGACTCCTCTGCTTCCTATGGAGCTCTCTCCTCTCTCCATAACCTCCGTCTCCCCGATTCAACATTCAAACGCTTGGCTACCCACCCTGAGTTTCTCTATTCATTGACGAATTTCATGAAGTTACAGGGTACTCGTGAATCGTCCAGAGCATACGTGGTGTTGATTTTGAAATCGATCTTCGAAGTCACTGATCCAATTCAACTAAGATTCTTAAATCCGATTAATTTGTGCAAATTGTTGAGATTTTGA

Coding sequence (CDS)

ATGGCTTCCCCACTCTCAAACCCCCCATCCATGAATCCAATCCTCTACCTCATTTCTACTGCCAACACTTCCCCTTCTTCCTTAGTCTCCTCCATCAGACGTCTCCGCTCTATCTCCGCCGACTCTAAAACCAACCGCTGTTTTGTCGACACCGACTCCTCTGCTTCCTATGGAGCTCTCTCCTCTCTCCATAACCTCCGTCTCCCCGATTCAACATTCAAACGCTTGGCTACCCACCCTGAGTTTCTCTATTCATTGACGAATTTCATGAAGTTACAGGGTACTCGTGAATCGTCCAGAGCATACGTGGTGTTGATTTTGAAATCGATCTTCGAAGTCACTGATCCAATTCAACTAAGATTCTTAAATCCGATTAATTTGTGCAAATTGTTGAGATTTTGA

Protein sequence

MASPLSNPPSMNPILYLISTANTSPSSLVSSIRRLRSISADSKTNRCFVDTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKLQGTRESSRAYVVLILKSIFEVTDPIQLRFLNPINLCKLLRF

Homology

[Close]

BLAST of Cla97C11G215200 vs. NCBI nr
Match: XP_038903290.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 152.5 bits (384), Expect = 2.5e-33
Identity = 92/134 (68.66%), Postives = 101/134 (75.37%), Query Frame = 0


Query: 4   PLSNPP-SMNPILYLISTANTSPSSLVSSIRRLRSISADSKTNR--------------CF 63
           P   PP   + IL+LIS+ANTSPSSL+SSI RLRSIS +S+TNR                
Sbjct: 90  PTPKPPIHKSQILHLISSANTSPSSLISSIHRLRSISDESETNRRCVESAGAPEFLASVI 149

Query: 64  VDTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKLQGTRESSRAYVVLILK 123
           VDTDSSASY ALS+LHNLRL DSTFKRLATH EFL SLTNFMKL+GT+ESSRAY VLILK
Sbjct: 150 VDTDSSASYEALSTLHNLRLSDSTFKRLATHSEFLDSLTNFMKLRGTQESSRAYAVLILK 209

[Close]

BLAST of Cla97C11G215200 vs. NCBI nr
Match: XP_008442921.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo] >KAA0043809.1 E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa] >TYK25323.1 E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa])

HSP 1 Score: 147.5 bits (371), Expect = 8.1e-32
Identity = 90/137 (65.69%), Postives = 101/137 (73.72%), Query Frame = 0


Query: 4   PLSNPP-SMNPILYLISTANTSPSSLVSSIRRLRSISADSKTNR--------------CF 63
           P   PP   + IL++IST+NTSPSSL+SSIRRLRSISA+S+TNR                
Sbjct: 90  PTPKPPIHKSQILHIISTSNTSPSSLISSIRRLRSISAESETNRRCVEFAGAPEFLVSVI 149

Query: 64  VDTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKL-QGTRESSRAYVVLIL 123
           VD+DSSAS+ ALS+LHNLRL DSTFK LATHPE L SLT+FMKL QGT ESSR Y VLIL
Sbjct: 150 VDSDSSASHEALSTLHNLRLSDSTFKSLATHPEVLESLTDFMKLQQGTHESSRTYAVLIL 209

Query: 124 KSIFEVTDPIQLRFLNP 125
           KSI EV DPIQL FL P
Sbjct: 210 KSIVEVADPIQLSFLKP 226

[Close]

BLAST of Cla97C11G215200 vs. NCBI nr
Match: XP_004136517.1 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN59215.1 hypothetical protein Csa_001842 [Cucumis sativus])

HSP 1 Score: 141.0 bits (354), Expect = 7.6e-30
Identity = 87/137 (63.50%), Postives = 99/137 (72.26%), Query Frame = 0


Query: 4   PLSNPP-SMNPILYLISTANTSPSSLVSSIRRLRSISADSKTNR--------------CF 63
           P   PP   + IL++IST+NTSPSS +SSIRRLRSISA+S+TNR                
Sbjct: 90  PTPKPPIHKSQILHIISTSNTSPSSQISSIRRLRSISAESETNRRCVEFAGAPEFLVSVI 149

Query: 64  VDTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKL-QGTRESSRAYVVLIL 123
           V +DSSAS+ ALS+LHNLRL DSTFK LAT PEFL SLT+FMKL QGT ESSR Y VLIL
Sbjct: 150 VGSDSSASHEALSTLHNLRLSDSTFKSLATRPEFLESLTDFMKLQQGTHESSRTYAVLIL 209

Query: 124 KSIFEVTDPIQLRFLNP 125
           KSI EV +PIQL FL P
Sbjct: 210 KSIIEVAEPIQLSFLKP 226

[Close]

BLAST of Cla97C11G215200 vs. NCBI nr
Match: XP_023540239.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 85.9 bits (211), Expect = 2.9e-13
Identity = 61/142 (42.96%), Postives = 85/142 (59.86%), Query Frame = 0


Query: 4   PLSNPPSMNPILY-LISTANTSPSSLVSSIRRLRSISADSKTNRCFV------------- 63
           P   PP   P ++ LIS+++++PSSL+S IRRLRS++A+S++NR  +             
Sbjct: 88  PTPKPPINKPQIHKLISSSSSTPSSLLSCIRRLRSLAAESESNRRCIESAGAPEFLASVI 147

Query: 64  ---------DTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKLQGTRESSR 123
                    +T S + + ALS+LHNLRL DS  K L  + E   SLT  M+ QGT E SR
Sbjct: 148 INSISDNDNETASISCHEALSTLHNLRLSDSKLKSLTVNAELFDSLTKVME-QGTYE-SR 207

[Close]

BLAST of Cla97C11G215200 vs. NCBI nr
Match: KAG6596619.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 85.9 bits (211), Expect = 2.9e-13
Identity = 61/142 (42.96%), Postives = 85/142 (59.86%), Query Frame = 0


Query: 4   PLSNPPSMNPILY-LISTANTSPSSLVSSIRRLRSISADSKTNRCFV------------- 63
           P   PP   P ++ LIS+++++PSSL+S IRRLRS++A+S++NR  +             
Sbjct: 88  PTPKPPINKPQIHKLISSSSSTPSSLLSCIRRLRSLAAESESNRRCIESAGAPEFLASVI 147

Query: 64  ---------DTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKLQGTRESSR 123
                    +T S + + ALS+LHNLRL DS  K L  + E   SLT  M+ QGT E SR
Sbjct: 148 INSISDNDNETASISCHEALSTLHNLRLSDSKLKSLTVNAELFDSLTKVME-QGTYE-SR 207

[Close]

BLAST of Cla97C11G215200 vs. ExPASy TrEMBL
Match: A0A5D3DNW0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004610 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.9e-32
Identity = 90/137 (65.69%), Postives = 101/137 (73.72%), Query Frame = 0


Query: 4   PLSNPP-SMNPILYLISTANTSPSSLVSSIRRLRSISADSKTNR--------------CF 63
           P   PP   + IL++IST+NTSPSSL+SSIRRLRSISA+S+TNR                
Sbjct: 90  PTPKPPIHKSQILHIISTSNTSPSSLISSIRRLRSISAESETNRRCVEFAGAPEFLVSVI 149

Query: 64  VDTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKL-QGTRESSRAYVVLIL 123
           VD+DSSAS+ ALS+LHNLRL DSTFK LATHPE L SLT+FMKL QGT ESSR Y VLIL
Sbjct: 150 VDSDSSASHEALSTLHNLRLSDSTFKSLATHPEVLESLTDFMKLQQGTHESSRTYAVLIL 209

Query: 124 KSIFEVTDPIQLRFLNP 125
           KSI EV DPIQL FL P
Sbjct: 210 KSIVEVADPIQLSFLKP 226

[Close]

BLAST of Cla97C11G215200 vs. ExPASy TrEMBL
Match: A0A1S3B6U7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486683 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.9e-32
Identity = 90/137 (65.69%), Postives = 101/137 (73.72%), Query Frame = 0


Query: 4   PLSNPP-SMNPILYLISTANTSPSSLVSSIRRLRSISADSKTNR--------------CF 63
           P   PP   + IL++IST+NTSPSSL+SSIRRLRSISA+S+TNR                
Sbjct: 90  PTPKPPIHKSQILHIISTSNTSPSSLISSIRRLRSISAESETNRRCVEFAGAPEFLVSVI 149

Query: 64  VDTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKL-QGTRESSRAYVVLIL 123
           VD+DSSAS+ ALS+LHNLRL DSTFK LATHPE L SLT+FMKL QGT ESSR Y VLIL
Sbjct: 150 VDSDSSASHEALSTLHNLRLSDSTFKSLATHPEVLESLTDFMKLQQGTHESSRTYAVLIL 209

Query: 124 KSIFEVTDPIQLRFLNP 125
           KSI EV DPIQL FL P
Sbjct: 210 KSIVEVADPIQLSFLKP 226

[Close]

BLAST of Cla97C11G215200 vs. ExPASy TrEMBL
Match: A0A0A0LB33 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G782630 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.7e-30
Identity = 87/137 (63.50%), Postives = 99/137 (72.26%), Query Frame = 0


Query: 4   PLSNPP-SMNPILYLISTANTSPSSLVSSIRRLRSISADSKTNR--------------CF 63
           P   PP   + IL++IST+NTSPSS +SSIRRLRSISA+S+TNR                
Sbjct: 90  PTPKPPIHKSQILHIISTSNTSPSSQISSIRRLRSISAESETNRRCVEFAGAPEFLVSVI 149

Query: 64  VDTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKL-QGTRESSRAYVVLIL 123
           V +DSSAS+ ALS+LHNLRL DSTFK LAT PEFL SLT+FMKL QGT ESSR Y VLIL
Sbjct: 150 VGSDSSASHEALSTLHNLRLSDSTFKSLATRPEFLESLTDFMKLQQGTHESSRTYAVLIL 209

Query: 124 KSIFEVTDPIQLRFLNP 125
           KSI EV +PIQL FL P
Sbjct: 210 KSIIEVAEPIQLSFLKP 226

[Close]

BLAST of Cla97C11G215200 vs. ExPASy TrEMBL
Match: A0A6J1KXX6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498577 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.1e-13
Identity = 61/142 (42.96%), Postives = 85/142 (59.86%), Query Frame = 0


Query: 4   PLSNPPSMNPILY-LISTANTSPSSLVSSIRRLRSISADSKTNRCFV------------- 63
           P   PP   P ++ LIS+++++PSSL+S IRRLRS++A+S++NR  +             
Sbjct: 88  PTPKPPINKPQIHKLISSSSSTPSSLLSCIRRLRSLAAESESNRRCIESAGAPEFLASVI 147

Query: 64  -----DTDSSAS----YGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKLQGTRESSR 123
                D D+  +    + ALS+LHNLRL DS  K L  + E   SLT  M+ QGT E SR
Sbjct: 148 INSISDNDNETALISCHEALSTLHNLRLSDSKLKSLTVNAELFDSLTKVME-QGTYE-SR 207

[Close]

BLAST of Cla97C11G215200 vs. ExPASy TrEMBL
Match: A0A6J1GKE4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111455050 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.1e-13
Identity = 60/142 (42.25%), Postives = 84/142 (59.15%), Query Frame = 0


Query: 4   PLSNPPSMNPILY-LISTANTSPSSLVSSIRRLRSISADSKTNRCFV------------- 63
           P   PP   P ++ LIS+++++PSSL+S IRRLRS++A+S++NR  +             
Sbjct: 88  PTPKPPINKPQIHKLISSSSSTPSSLLSCIRRLRSLAAESESNRRCIESAGAPEFLASVI 147

Query: 64  ---------DTDSSASYGALSSLHNLRLPDSTFKRLATHPEFLYSLTNFMKLQGTRESSR 123
                    +T S + + ALS+LHNLRL DS  K L  + E   SLT  M+ QGT E SR
Sbjct: 148 INSISDNDNETASISCHEALSTLHNLRLSDSKLKSLTVNAELFDSLTKVME-QGTYE-SR 207

[Close]

BLAST of Cla97C11G215200 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 41.6 bits (96), Expect = 5.8e-04
Identity = 46/141 (32.62%), Postives = 69/141 (48.94%), Query Frame = 0


Query: 4   PLSNPP-SMNPILYLISTANTSPSSLVSSIRRLRSISADSKTN-RC------------FV 63
           P   PP   + I  LI  + +S  + V  ++RLR I +++ TN RC             V
Sbjct: 92  PTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSENATNKRCLEAAGVPEFLANIV 151

Query: 64  DTDS---SASYGALSSLHNLRLPDSTFKRLATHPE---FLYSLTNFMKLQGTRESSRAYV 123
             DS   S +  AL+ L++L   ++  K L  + +    + SLT  M+ +G  E SR Y 
Sbjct: 152 SNDSENGSLTDEALNLLYHLETSETVLKNLLNNKKDNNIVKSLTKIMQ-RGMYE-SRVYA 211

Query: 124 VLILKSIFEVTDPIQLRFLNP 125
            L+LK+I EV DP+Q   L P
Sbjct: 212 TLLLKNILEVADPMQSMTLKP 230

The following BLAST results are available for this feature:

BLAST of Cla97C11G215200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903290.1	2.5e-33	68.66	E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida]	[more]
XP_008442921.1	8.1e-32	65.69	PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo] >KAA0043809.1 E...	[more]
XP_004136517.1	7.6e-30	63.50	E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN59215.1 hypothetical pro...	[more]
XP_023540239.1	2.9e-13	42.96	E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo]	[more]
KAG6596619.1	2.9e-13	42.96	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Cla97C11G215200 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Cla97C11G215200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DNW0	3.9e-32	65.69	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B6U7	3.9e-32	65.69	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486683 PE=4 ...	[more]
A0A0A0LB33	3.7e-30	63.50	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G782630 PE...	[more]
A0A6J1KXX6	4.1e-13	42.96	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498577 P...	[more]
A0A6J1GKE4	4.1e-13	42.25	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111455050...	[more]

BLAST of Cla97C11G215200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	5.8e-04	32.62	plant U-box 23	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 54..124
None	No IPR available	PANTHER	PTHR22849:SF132	E3 UBIQUITIN-PROTEIN LIGASE PUB23	coord: 54..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C11G215200.1	Cla97C11G215200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016740	transferase activity