Cla97C10G189870 (gene) Watermelon (97103) v2.5

Overview
NameCla97C10G189870
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionThiamine thiazole synthase, chloroplastic
LocationCla97Chr10: 6232260 .. 6233794 (-)
RNA-Seq ExpressionCla97C10G189870
SyntenyCla97C10G189870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAGGCCTTCCTTACTTCTTGACTCCTCTTTCCATGGCACTCCTTTGGCCTCTCCTCATTCTCTCCGCCTCAAATCCACCACCGCCGCCGCCGCCGCATCTCTCTCCATCTCCGCCTCCGCCTCTCAGCTCCCCTACGATTTGAACCAATTCAAATTCAACCCCATCAAGGAATCCATCGTCTCCCGGGAAATGACCCGCCGCTACATGACCGACATGATCACCTACGCCGATACCGACGTCATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAGCTCAGCAAAAACCCTTCTATTCGCATCGCCATCATCGAACAGTCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTAATAAATTCATTTCCTTTCCATATATGCCTTCTCATTTCCTTCCCTTCTCCATATTTGGACGTATGCTCTGTTTTTTTGTTCTTTTCTTGAAAATACCAAAAATTTCAAAACCCCCCACCCAGCTTTCATGCATGTCGTATTTGGTCTCAAGATTTCATCCTTTTGAACAGAGATCTGAAAGAAAAAAAGATGTTAAACCACGAAGTTTCTTAACGATTCTGGGAATTTTTATTCAGATCTTATGATGCTCTGATAAACTAGATTCAATTTCATTAAGAAATTTTCTGATCTTATTAATCTCATAGATTTGGTTTGAATTTAAGTTTTGATTAAAATTGTTTGACAGGTTGTACGGAAACCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGACGACTACGTTGTGATAAAGCACGCAGCTCTGTTCACCTCAACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCTGCGGAGGACTTGATCGTTAAGGGCGGCAGAGTCGGCGGTGTCGTCACCAACTGGGCGCTGGTGTCCATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTTAGCTCTTGTGGCCACGACGGACCCTTCGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGACAGCGTCCCCGGAATGAAGGCCCTTGACATGAACACAGCCGAGGATGCCATTGTTAGATTCACCCGAGAGGTGGTTCCCGGCATGATTGTCACCGGGATGGAGGTTGCGGAGATCGATGGAGCTCCAAGAATGGTAAACCAGCTAGAATTGATAATGATTTTGTTTAGATTTATTTTTGTTAGTCCTAAAAGTATTATGTATTTGACGTGGGATGCAGGGGCCGACGTTCGGGGCGATGATGATATCGGGGCAGAAGGCGGCGCACTTGGCGCTGAAGTCATTGGGAGAGGCGAACAGCATTGAGGGTGAAGAAGAAAGAAGGGATGCACATGGAGTGGGGGTGAGTGAAGAGGAAGAACCAGAGCTGCTACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGA

mRNA sequence

ATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAGGCCTTCCTTACTTCTTGACTCCTCTTTCCATGGCACTCCTTTGGCCTCTCCTCATTCTCTCCGCCTCAAATCCACCACCGCCGCCGCCGCCGCATCTCTCTCCATCTCCGCCTCCGCCTCTCAGCTCCCCTACGATTTGAACCAATTCAAATTCAACCCCATCAAGGAATCCATCGTCTCCCGGGAAATGACCCGCCGCTACATGACCGACATGATCACCTACGCCGATACCGACGTCATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAGCTCAGCAAAAACCCTTCTATTCGCATCGCCATCATCGAACAGTCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTTGTACGGAAACCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGACGACTACGTTGTGATAAAGCACGCAGCTCTGTTCACCTCAACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCTGCGGAGGACTTGATCGTTAAGGGCGGCAGAGTCGGCGGTGTCGTCACCAACTGGGCGCTGGTGTCCATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTTAGCTCTTGTGGCCACGACGGACCCTTCGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGACAGCGTCCCCGGAATGAAGGCCCTTGACATGAACACAGCCGAGGATGCCATTGTTAGATTCACCCGAGAGGTGGTTCCCGGCATGATTGTCACCGGGATGGAGGTTGCGGAGATCGATGGAGCTCCAAGAATGGGGCCGACGTTCGGGGCGATGATGATATCGGGGCAGAAGGCGGCGCACTTGGCGCTGAAGTCATTGGGAGAGGCGAACAGCATTGAGGGTGAAGAAGAAAGAAGGGATGCACATGGAGTGGGGGTGAGTGAAGAGGAAGAACCAGAGCTGCTACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGA

Coding sequence (CDS)

ATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAGGCCTTCCTTACTTCTTGACTCCTCTTTCCATGGCACTCCTTTGGCCTCTCCTCATTCTCTCCGCCTCAAATCCACCACCGCCGCCGCCGCCGCATCTCTCTCCATCTCCGCCTCCGCCTCTCAGCTCCCCTACGATTTGAACCAATTCAAATTCAACCCCATCAAGGAATCCATCGTCTCCCGGGAAATGACCCGCCGCTACATGACCGACATGATCACCTACGCCGATACCGACGTCATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAGCTCAGCAAAAACCCTTCTATTCGCATCGCCATCATCGAACAGTCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTTGTACGGAAACCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGACGACTACGTTGTGATAAAGCACGCAGCTCTGTTCACCTCAACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCTGCGGAGGACTTGATCGTTAAGGGCGGCAGAGTCGGCGGTGTCGTCACCAACTGGGCGCTGGTGTCCATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTTAGCTCTTGTGGCCACGACGGACCCTTCGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGACAGCGTCCCCGGAATGAAGGCCCTTGACATGAACACAGCCGAGGATGCCATTGTTAGATTCACCCGAGAGGTGGTTCCCGGCATGATTGTCACCGGGATGGAGGTTGCGGAGATCGATGGAGCTCCAAGAATGGGGCCGACGTTCGGGGCGATGATGATATCGGGGCAGAAGGCGGCGCACTTGGCGCTGAAGTCATTGGGAGAGGCGAACAGCATTGAGGGTGAAGAAGAAAGAAGGGATGCACATGGAGTGGGGGTGAGTGAAGAGGAAGAACCAGAGCTGCTACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGA

Protein sequence

MASIASTLTTKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGESPEVADA
Homology
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BLAST of Cla97C10G189870 vs. NCBI nr
Match: XP_038905461.1 (thiamine thiazole synthase, chloroplastic [Benincasa hispida])

HSP 1 Score: 638.6 bits (1646), Expect = 3.2e-179
Identity = 344/368 (93.48%), Postives = 346/368 (94.02%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPLASPHSLRLKS-TTAAAAASLSISASASQLPYD 60
           MASIASTLTTKLQRPSLLLDSSF+GTPLA P SLRLKS TT  AA SLSISASASQ PYD
Sbjct: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSLRLKSTTTTTAATSLSISASASQPPYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI
Sbjct: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIE-GEEERRDAHGVGVSEEEEPELLLAAG 360
           APRMGPTFGAMMISGQKAAHLALKSLGEAN IE GE   R          EEPELLLAAG
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKV-------VEEPELLLAAG 360

Query: 361 ESPEVADA 367
           ESPEVADA
Sbjct: 361 ESPEVADA 361

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BLAST of Cla97C10G189870 vs. NCBI nr
Match: XP_004152916.1 (thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothetical protein Csa_011133 [Cucumis sativus])

HSP 1 Score: 637.1 bits (1642), Expect = 9.3e-179
Identity = 339/366 (92.62%), Postives = 347/366 (94.81%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDL 60
           MASIASTLTTKLQ+PSLL DSSFHGTPLASP SLRLKST   AA SL+ISASASQ PYDL
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKST---AATSLTISASASQPPYDL 60

Query: 61  NQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAII 120
           NQFKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAII
Sbjct: 61  NQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGES 360
           PRMGPTFGAMMISGQKAAHLALKSLGEANSI G+E       VG+ EE   ELLLAAGES
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSI-GDEGEMGERKVGLEEE---ELLLAAGES 359

Query: 361 PEVADA 367
           PEVADA
Sbjct: 361 PEVADA 359

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BLAST of Cla97C10G189870 vs. NCBI nr
Match: XP_008463440.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 634.4 bits (1635), Expect = 6.0e-178
Identity = 341/366 (93.17%), Postives = 346/366 (94.54%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDL 60
           MASIASTLTTKLQ PSLL DSSFHGTPLASP SLRLKS    AA SL+ISASASQ PYDL
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKS---PAATSLTISASASQPPYDL 60

Query: 61  NQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAII 120
           NQFKFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNP+IRIAII
Sbjct: 61  NQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGES 360
           PRMGPTFGAMMISGQKAAHLALKSLGEANSI GE E  +   VG+ EE   ELLLAAGES
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGE-RKVGLEEE---ELLLAAGES 359

Query: 361 PEVADA 367
           PEVADA
Sbjct: 361 PEVADA 359

[Close]
BLAST of Cla97C10G189870 vs. NCBI nr
Match: WP_156424216.1 (sulfide-dependent adenosine diphosphate thiazole synthase, partial [Myroides odoratus])

HSP 1 Score: 615.1 bits (1585), Expect = 3.8e-172
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0 

Query: 10  TKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDLNQFKFNPIK 69
           TKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDLNQFKFNPIK
Sbjct: 1   TKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDLNQFKFNPIK 60

Query: 70  ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAIIEQSVSPGGG 129
           ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAIIEQSVSPGGG
Sbjct: 61  ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAIIEQSVSPGGG 120

Query: 130 AWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLARPNVKLF 189
           AWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLARPNVKLF
Sbjct: 121 AWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLARPNVKLF 180

Query: 190 NAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGV 249
           NAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGV
Sbjct: 181 NAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGV 240

Query: 250 KRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGA 309
           KRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGA
Sbjct: 241 KRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGA 300

Query: 310 MMISGQKAAHLALKSL 326
           MMISGQKAAHLALKSL
Sbjct: 301 MMISGQKAAHLALKSL 316

[Close]
BLAST of Cla97C10G189870 vs. NCBI nr
Match: KAG7031400.1 (Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 597.4 bits (1539), Expect = 8.1e-167
Identity = 323/370 (87.30%), Postives = 330/370 (89.19%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRP----SLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASAS-Q 60
           MAS+ASTLTTKLQ P    S LLDSSFHGTPLA P SLRLK T     AS SISASAS Q
Sbjct: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPT----PASFSISASASQQ 60

Query: 61  LPYDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSI 120
            PYDLN FKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP I
Sbjct: 61  QPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDI 120

Query: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTST 180
           RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTST
Sbjct: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTST 180

Query: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240
           IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV
Sbjct: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240

Query: 241 SSCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVA 300
           SSCGHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVA
Sbjct: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVA 300

Query: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLL 360
           EIDGAPRMGPTFGAMMISGQKAAHLALKSLG AN I               E+E  +L+L
Sbjct: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCI---------------EDEAADLIL 351

Query: 361 AAGESPEVAD 366
           AAGESPEVAD
Sbjct: 361 AAGESPEVAD 351

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BLAST of Cla97C10G189870 vs. ExPASy Swiss-Prot
Match: O23787 (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.0e-156
Identity = 295/368 (80.16%), Postives = 324/368 (88.04%), Query Frame = 0 

Query: 1   MASIAST--LTTKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPY 60
           MA++AST    +     + L DSSFHG P+ SP  LRL+   ++   +LSISASAS  PY
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPM-SPSLLRLQPIKSSRPNNLSISASASP-PY 60

Query: 61  DLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIA 120
           DLN FKF+PIKESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNP+I+IA
Sbjct: 61  DLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+G++YDEQD+YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+I+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAG 360
           GAPRMGPTFGAMMISGQKAAHLALKSLG+ N+++G       +  GV     PEL+LAA 
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDG------TYVGGV----HPELILAAA 356

Query: 361 ESPEVADA 367
           +S E ADA
Sbjct: 361 DSAETADA 356

[Close]
BLAST of Cla97C10G189870 vs. ExPASy Swiss-Prot
Match: Q38709 (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 3.4e-152
Identity = 286/365 (78.36%), Postives = 321/365 (87.95%), Query Frame = 0 

Query: 4   IASTLTTKLQRPSLLLD--SSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDLN 63
           +ASTLT+K QR +L  +  SSF+GTPLA P S+R++ T A A  S+S+S + S  PYDL 
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPSP-PYDLK 60

Query: 64  QFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAIIE 123
            F F+PIKESIVSREMTRRYM DMITYADTDV++VGAGS+GL C YELSKNPS+++AIIE
Sbjct: 61  AFTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIE 120

Query: 124 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 183
           QSVSPGGGAWLGGQLFS MVVRKPAHLFLDE+G+EYDEQD+YVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLL 180

Query: 184 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 243
           ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 244 GPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 303
           GPFGATGVK L+SIG+ID+VPGMKALDMN AEDAIVR TRE+VPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAP 300

Query: 304 RMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGESP 363
           RMGPTFGAMMISGQKAAHLALK+LG  N+++G      ++  G+     PEL+LAA +S 
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKALGLPNALDG------SYVGGI----HPELILAAADSA 352

Query: 364 EVADA 367
           E+ADA
Sbjct: 361 EIADA 352

[Close]
BLAST of Cla97C10G189870 vs. ExPASy Swiss-Prot
Match: F6H7K5 (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 4.2e-150
Identity = 285/369 (77.24%), Postives = 312/369 (84.55%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLL---DSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLP 60
           MAS+A+TLT+    P        SSFHG+P+ S   L +K +  +   S+S     S  P
Sbjct: 1   MASMATTLTSLSSNPKPAFFDNKSSFHGSPI-SYRVLPIKVSHQSPTISMS-----SVSP 60

Query: 61  YDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRI 120
           YDL  FKF PIKESIV+REMTRRYM DMITYADTDV+IVGAGSAGLSCAYELSKNPSIR+
Sbjct: 61  YDLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRV 120

Query: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIM 180
           AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIM
Sbjct: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIM 180

Query: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240
           SKLLARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS
Sbjct: 181 SKLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240

Query: 241 CGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEI 300
           CGHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEI
Sbjct: 241 CGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEI 300

Query: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAA 360
           DGAPRMGPTFGAMMISGQKAAHLAL++LG+ N+I+G            +E  +PEL+LAA
Sbjct: 301 DGAPRMGPTFGAMMISGQKAAHLALRALGQPNAIDGNYTE--------AETMQPELILAA 355

Query: 361 GESPEVADA 367
            E+ E+ DA
Sbjct: 361 AETGEIVDA 355

[Close]
BLAST of Cla97C10G189870 vs. ExPASy Swiss-Prot
Match: Q38814 (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 6.7e-148
Identity = 277/367 (75.48%), Postives = 306/367 (83.38%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPL-ASPHSLRLKSTTAAAAASLSISASASQLPYD 60
           MA+IASTL+    +P  L DSSFHG+ + A+P S+ LK          S S  A+   YD
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPR--------SFSVRATTAGYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LN F F+PIKESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNP++++AI
Sbjct: 61  LNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQD YVV+KHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGE 360
           APRMGPTFGAMMISGQKA  LALK+LG  N+I+G           +     PEL+LAA +
Sbjct: 301 APRMGPTFGAMMISGQKAGQLALKALGLPNAIDGT----------LVGNLSPELVLAAAD 349

Query: 361 SPEVADA 367
           S E  DA
Sbjct: 361 SAETVDA 349

[Close]
BLAST of Cla97C10G189870 vs. ExPASy Swiss-Prot
Match: F6H9A9 (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 4.8e-146
Identity = 276/371 (74.39%), Postives = 318/371 (85.71%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLD---SSFHGTPLASPHSLR-LKSTTAAAAASLSISASASQL 60
           MA++ S++ +K +  + + D   SSFHG P+A+   L  +KST       ++++ +A+ +
Sbjct: 1   MATLTSSICSKPK--ASVFDPHKSSFHGVPIATQARLSPVKST------PVNLAVTAAAM 60

Query: 61  PYDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIR 120
           PYDL  FKF PIKESIVSREMTRRYM DMITYADTDV++VGAGSAGLSCAYELSKNPS++
Sbjct: 61  PYDLRSFKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTI 180
           +AIIEQSVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTI
Sbjct: 121 VAIIEQSVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRL+S+G+IDSVPGMKALDMNTAED IVR TREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLL- 360
           IDG+PRMGPTFGAMMISGQKAAHLALKSLG  N+++G         +G      PEL+L 
Sbjct: 301 IDGSPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGTY-------IG---NLHPELVLA 353

Query: 361 AAGESPEVADA 367
           AA ++ E+A+A
Sbjct: 361 AAADAAEIAEA 353

[Close]
BLAST of Cla97C10G189870 vs. ExPASy TrEMBL
Match: A0A0A0L546 (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.5e-179
Identity = 339/366 (92.62%), Postives = 347/366 (94.81%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDL 60
           MASIASTLTTKLQ+PSLL DSSFHGTPLASP SLRLKST   AA SL+ISASASQ PYDL
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKST---AATSLTISASASQPPYDL 60

Query: 61  NQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAII 120
           NQFKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAII
Sbjct: 61  NQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGES 360
           PRMGPTFGAMMISGQKAAHLALKSLGEANSI G+E       VG+ EE   ELLLAAGES
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSI-GDEGEMGERKVGLEEE---ELLLAAGES 359

Query: 361 PEVADA 367
           PEVADA
Sbjct: 361 PEVADA 359

[Close]
BLAST of Cla97C10G189870 vs. ExPASy TrEMBL
Match: A0A1S3CJ81 (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.9e-178
Identity = 341/366 (93.17%), Postives = 346/366 (94.54%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDL 60
           MASIASTLTTKLQ PSLL DSSFHGTPLASP SLRLKS    AA SL+ISASASQ PYDL
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKS---PAATSLTISASASQPPYDL 60

Query: 61  NQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAII 120
           NQFKFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNP+IRIAII
Sbjct: 61  NQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGES 360
           PRMGPTFGAMMISGQKAAHLALKSLGEANSI GE E  +   VG+ EE   ELLLAAGES
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGE-RKVGLEEE---ELLLAAGES 359

Query: 361 PEVADA 367
           PEVADA
Sbjct: 361 PEVADA 359

[Close]
BLAST of Cla97C10G189870 vs. ExPASy TrEMBL
Match: A0A6J1J7L4 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483293 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.9e-167
Identity = 318/366 (86.89%), Postives = 337/366 (92.08%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDL 60
           MASIAS+L TKL RPS LLDSSFHG+PL    S RLK TT  AAASLSISASA+Q PYDL
Sbjct: 1   MASIASSLPTKLHRPS-LLDSSFHGSPLLPTPSFRLKPTT--AAASLSISASAAQPPYDL 60

Query: 61  NQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAII 120
           NQFKFNPI+ESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+PS+R+AII
Sbjct: 61  NQFKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGES 360
           PRMGPTFGAMMISGQKAAHLALK+LG+ANSI+ + E       G  + E  E++LAAGE+
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKALGKANSIDEKGEN------GGRKVEAAEMVLAAGET 357

Query: 361 PEVADA 367
           PEVADA
Sbjct: 361 PEVADA 357

[Close]
BLAST of Cla97C10G189870 vs. ExPASy TrEMBL
Match: A0A6J1FWL6 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448035 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.4e-166
Identity = 322/367 (87.74%), Postives = 329/367 (89.65%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRP-SLLLDSSFHGTPLA-SPHSLRLKSTTAAAAASLSISASASQLPY 60
           MAS+ASTLTTKLQ P S LLDSSFHGTPLA  P SLRLKST AA     SISAS  Q PY
Sbjct: 1   MASVASTLTTKLQTPSSSLLDSSFHGTPLAPPPPSLRLKSTPAA----FSISASQQQ-PY 60

Query: 61  DLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIA 120
           DLN FKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP IRIA
Sbjct: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAG 360
           GAPRMGPTFGAMMISGQKAAHLALKSLG AN I               E+E  +L+LAAG
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCI---------------EDEAADLILAAG 347

Query: 361 ESPEVAD 366
           ESPEVAD
Sbjct: 361 ESPEVAD 347

[Close]
BLAST of Cla97C10G189870 vs. ExPASy TrEMBL
Match: A0A6J1EVU2 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438464 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.8e-165
Identity = 314/366 (85.79%), Postives = 333/366 (90.98%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPLASPHSLRLKSTTAAAAASLSISASASQLPYDL 60
           MASIAS+L  KL RPS LL+SSFHG+PL    S RLK TT  AAASLSISASA+Q PYDL
Sbjct: 1   MASIASSLPIKLHRPS-LLESSFHGSPLLPTPSFRLKPTT--AAASLSISASAAQPPYDL 60

Query: 61  NQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAII 120
           NQFKFNPI+ESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+PS+R+AII
Sbjct: 61  NQFKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIG+IDSVPGMKALDMN+AEDAIVRFTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNSAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGES 360
           PRMGPTFGAMMISGQKAAHLALK+LG+ NSI+G             E E  E++LAAGE+
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKALGKTNSIDG-------------EVEAAEMVLAAGET 350

Query: 361 PEVADA 367
           PEVADA
Sbjct: 361 PEVADA 350

[Close]
BLAST of Cla97C10G189870 vs. TAIR 10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) )

HSP 1 Score: 525.0 bits (1351), Expect = 4.8e-149
Identity = 277/367 (75.48%), Postives = 306/367 (83.38%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFHGTPL-ASPHSLRLKSTTAAAAASLSISASASQLPYD 60
           MA+IASTL+    +P  L DSSFHG+ + A+P S+ LK          S S  A+   YD
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPR--------SFSVRATTAGYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LN F F+PIKESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNP++++AI
Sbjct: 61  LNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQD YVV+KHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIEGEEERRDAHGVGVSEEEEPELLLAAGE 360
           APRMGPTFGAMMISGQKA  LALK+LG  N+I+G           +     PEL+LAA +
Sbjct: 301 APRMGPTFGAMMISGQKAGQLALKALGLPNAIDGT----------LVGNLSPELVLAAAD 349

Query: 361 SPEVADA 367
           S E  DA
Sbjct: 361 SAETVDA 349

The following BLAST results are available for this feature:
BLAST of Cla97C10G189870 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038905461.13.2e-17993.48thiamine thiazole synthase, chloroplastic [Benincasa hispida][more]
XP_004152916.19.3e-17992.62thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothet... [more]
XP_008463440.16.0e-17893.17PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo][more]
WP_156424216.13.8e-172100.00sulfide-dependent adenosine diphosphate thiazole synthase, partial [Myroides odo... [more]
KAG7031400.18.1e-16787.30Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyr... [more]
BLAST of Cla97C10G189870 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O237871.0e-15680.16Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=... [more]
Q387093.4e-15278.36Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=... [more]
F6H7K54.2e-15077.24Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2... [more]
Q388146.7e-14875.48Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI... [more]
F6H9A94.8e-14674.39Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1... [more]
BLAST of Cla97C10G189870 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L5464.5e-17992.62Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=... [more]
A0A1S3CJ812.9e-17893.17Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160... [more]
A0A6J1J7L43.9e-16786.89Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FWL64.4e-16687.74Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1EVU22.8e-16585.79Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
BLAST of Cla97C10G189870 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G54770.14.8e-14975.48thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01946Thi4coord: 74..307
e-value: 3.2E-114
score: 380.0
NoneNo IPR availableGENE3D6.10.250.2840coord: 59..89
e-value: 2.9E-19
score: 70.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 328..366
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 329..348
NoneNo IPR availablePANTHERPTHR43422THIAMINE THIAZOLE SYNTHASEcoord: 1..366
NoneNo IPR availablePANTHERPTHR43422:SF11THIAMINE THIAZOLE SYNTHASE, CHLOROPLASTICcoord: 1..366
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 90..325
e-value: 7.2E-122
score: 407.1
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 53..319
IPR002922Thiazole biosynthetic enzyme Thi4 familyTIGRFAMTIGR00292TIGR00292coord: 70..325
e-value: 2.0E-82
score: 274.2
IPR027495Thiamine thiazole synthaseHAMAPMF_03158THI4coord: 5..334
score: 68.671906

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C10G189870.1Cla97C10G189870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009228 thiamine biosynthetic process
biological_process GO:0052837 thiazole biosynthetic process
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005829 cytosol
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0016763 pentosyltransferase activity