Cla97C09G171880 (gene) Watermelon (97103) v2.5

Overview
NameCla97C09G171880
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description3-methyl-2-oxobutanoate hydroxymethyltransferase
LocationCla97Chr09: 8298777 .. 8301555 (+)
RNA-Seq ExpressionCla97C09G171880
SyntenyCla97C09G171880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTCTTCAAACTCTTTACCGTGGCCGCACCTCGAACCCGAACGCAATCGCTTCTCAAAGTGCTTCGTAACATGAGCAATGTCCCTGAAAACACGGTTTACGGCGGCCCCAAGTCACAGAGTCCAAACCAGAGAGTCACTCTCACCAATCTGAGACAAAAGTACAAGAAAGGTGAACCGATCACCATGGTCACTGCTTACGATTACCCATCCGGAGTCCATTTGGACATGGCCGGCATTGATGTTGCTTTGGTCGGTGATTCGGCTGCCATGGTAGTGCACGGCCACGACACCACGTTGCCTATTAGCTTGGATGAAATGCTCGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCACTTTTGGTCGGTGATTTGCCCTTTGGTACTTATGAATCCAGCTCCGTACAGGTTTTTTTCATCTTTTGCCTTTTAGGTGTTTGAAGAAATGACAGCCATCTGGTTTTATTCATTTGAAGTTAGAGAAAGTCTGTTTTTTGTCGTTCTTTGAATGGACTGTACGGGAATCTATGGGATTCATTAGATTGTAAAACGTAAATGAAATTCCAAGCTCTTGTGAGAATGTCTACTTTTACTTGCTGATGGAGTTATCTAAACGATTGAAAATGAAAGTTCTTCAATTCATATCAAAATTATGTCTCAAAATGCATGAACAAACCGAACTTACTTTTTTTTTTTTTTTTTTTTTTTGCTATTGTTTTTACGATTATCACTTAGTGTAAATGCTCCGCTCCACTTTTCCATTTGAGAGTCTTTTGTGACTCCTTTCAAGCAGAGTTTTTACCCTTTTCTTTGAAATTAATAAAATTGATTCTCATATTAACTATTAAAGGGAAAAAAAAGCATAAACATGAAGGAACTAATCTTGTGGAATAATTTAGTATGGTAACTCTGATGAAAACTAGTTTAGAAATGTAAGAACTTATCTTTGATGGGAAACTTCAGTGTGGTTGTTGCCTGTAATTGAATCGTACGAGCAACTTTCTTTTTTAGGATGTAAAACCTTAAATGAATCAATTTGAACACCTCAGTTTTTAAAATGATTCAGAACTGTGTTGCTATTATTGATAGGCTGTTGATACGGCAGTAAGAATTCTGAAGGATGGGGGAATGGATGCAATAAAATTGGAAGGTGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCGGGAATTGCGGTAATGGGGCACGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAGGAATATTGCCAGTGCTGTTGAGGTTCTCCCACTGCTCTACTTGTTTCTTCCTCTTAGTGTACTGTTTGCAGCTGTTTCTGGGACACCATTTCTGTTGTTATGTTGCATAGTTTGTCATGTTTGTTTTGGCTTTTATGCTGAATAGTGGATCTTCGCAGGTGGTGGAAACTGCAATATCTCTACAGGAAGCTGGGTGTTTCGCTGTCGTTCTAGAATGTGTTCCTCCGGAAGTTGCAGCTGCAGCTACAGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTTAGTTGACTGCAGACATCCTTATACATGCCATCCATACGTACATATATACGTGCATATGTGCGTGCGTACGTACGTACGTACATATATATATACACATATATATATACACATACATATATATACACATATATATATATACATACATATATATACATACATACATATATATATATATATATGATGACTGCCAACCGTATTTCCTTTCCTTGAGCTATTTGTTTGTTTCTCTTTGTTTGATCTGGCATATAATTGTACCTTCAAACTATTTTTTTACCCAAGTTCAGGAGTTGGAGGCTCTCCCCCGAGTTCCCAGTGAGAGAAGCACCGAAGGTTGTCCTTGTTTAGTTTTCCAAAATGGTTCTTCAATTCTAAGCAGAATGAAATTTGAATGGACTCTTAACTGTTTAAATTGTTTTGAGAGAGATTCCCTAGAGGGAGTGGATTCACTAGAACATAGAAGACATTCAAGAGTAGAGAAATATTTCTACTGGTAAACAGCAAGCTTGCTAACAAACCATGGAGCAGCGAACTAATAATTTGTTAATGTTCGGATTTGAATGCCTGCTCTTTCAAATTGGAAGGAGCTTGTGAAGAAGAAAACAGACCTTGCATTTCAGTCAATAAAGAGACAGGGTTTATGATTTATTCAATATTTCAGACAACTTGTTGGTTTTTCATTTTTTGTTTTCAGAAATGTTGGTTTCTTCATCCATACTTGTTTGATAAGGTTGTTTGTTGTGTTGCTTGTATTGTCTTTGATTACTTGAAAACAATCATAGTGTTGAATAGCCTCTGCAGTCAGACTAATTAATTTCTTTAACTCAGGTGCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCGCATCATGCTAAGGTTACCATCAGGCTGTACCTTTGTTTGGTATAAGTTTCTCTATGTTTGTTTAAGATGTTTTGACTCCTGGATTATATATGCCCTTTGCAGGTGACCCCAAAATTCTGTAAGCAATTTGCTCAAATTGGAGATGTCATCAACAGAGCTTTATCAGATTACAGGGAAGAAGTATCTAATGGTTCGTTTCCCGGACCACTCCATAGCCCATATAAGATCAGCGATGATGATTTGAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATGA

mRNA sequence

ATGGCGCTCTTCAAACTCTTTACCGTGGCCGCACCTCGAACCCGAACGCAATCGCTTCTCAAAGTGCTTCGTAACATGAGCAATGTCCCTGAAAACACGGTTTACGGCGGCCCCAAGTCACAGAGTCCAAACCAGAGAGTCACTCTCACCAATCTGAGACAAAAGTACAAGAAAGGTGAACCGATCACCATGGTCACTGCTTACGATTACCCATCCGGAGTCCATTTGGACATGGCCGGCATTGATGTTGCTTTGGTCGGTGATTCGGCTGCCATGGTAGTGCACGGCCACGACACCACGTTGCCTATTAGCTTGGATGAAATGCTCGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCACTTTTGGTCGGTGATTTGCCCTTTGGTACTTATGAATCCAGCTCCGTACAGGCTGTTGATACGGCAGTAAGAATTCTGAAGGATGGGGGAATGGATGCAATAAAATTGGAAGGTGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCGGGAATTGCGGTAATGGGGCACGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAGGAATATTGCCAGTGCTGTTGAGGTGGTGGAAACTGCAATATCTCTACAGGAAGCTGGGTGTTTCGCTGTCGTTCTAGAATGTGTTCCTCCGGAAGTTGCAGCTGCAGCTACAGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCGCATCATGCTAAGGTGACCCCAAAATTCTGTAAGCAATTTGCTCAAATTGGAGATGTCATCAACAGAGCTTTATCAGATTACAGGGAAGAAGTATCTAATGGTTCGTTTCCCGGACCACTCCATAGCCCATATAAGATCAGCGATGATGATTTGAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATGA

Coding sequence (CDS)

ATGGCGCTCTTCAAACTCTTTACCGTGGCCGCACCTCGAACCCGAACGCAATCGCTTCTCAAAGTGCTTCGTAACATGAGCAATGTCCCTGAAAACACGGTTTACGGCGGCCCCAAGTCACAGAGTCCAAACCAGAGAGTCACTCTCACCAATCTGAGACAAAAGTACAAGAAAGGTGAACCGATCACCATGGTCACTGCTTACGATTACCCATCCGGAGTCCATTTGGACATGGCCGGCATTGATGTTGCTTTGGTCGGTGATTCGGCTGCCATGGTAGTGCACGGCCACGACACCACGTTGCCTATTAGCTTGGATGAAATGCTCGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCACTTTTGGTCGGTGATTTGCCCTTTGGTACTTATGAATCCAGCTCCGTACAGGCTGTTGATACGGCAGTAAGAATTCTGAAGGATGGGGGAATGGATGCAATAAAATTGGAAGGTGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCGGGAATTGCGGTAATGGGGCACGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAGGAATATTGCCAGTGCTGTTGAGGTGGTGGAAACTGCAATATCTCTACAGGAAGCTGGGTGTTTCGCTGTCGTTCTAGAATGTGTTCCTCCGGAAGTTGCAGCTGCAGCTACAGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCGCATCATGCTAAGGTGACCCCAAAATTCTGTAAGCAATTTGCTCAAATTGGAGATGTCATCAACAGAGCTTTATCAGATTACAGGGAAGAAGTATCTAATGGTTCGTTTCCCGGACCACTCCATAGCCCATATAAGATCAGCGATGATGATTTGAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATGA

Protein sequence

MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYREEVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR
Homology
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BLAST of Cla97C09G171880 vs. NCBI nr
Match: XP_038898896.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 640.6 bits (1651), Expect = 7.9e-180
Identity = 324/347 (93.37%), Postives = 337/347 (97.12%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MA+F LF+ AA RTRT+ LLK LRNMSNVPEN VYGGP SQ+PN RVTLTNLRQKYKKGE
Sbjct: 1   MAIFNLFSKAASRTRTRPLLKTLRNMSNVPENAVYGGPTSQTPNHRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSAAMVVHG+DTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGYDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLVGDLPFGTYESSSVQAVDTAVR+LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NIASAV+VVETAISLQEAGCFAVVLECVPPEVAAAAT+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATSA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+ALS+YRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D NGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDTDFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of Cla97C09G171880 vs. NCBI nr
Match: XP_004139651.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KGN44587.1 hypothetical protein Csa_016545 [Cucumis sativus])

HSP 1 Score: 639.8 bits (1649), Expect = 1.4e-179
Identity = 323/347 (93.08%), Postives = 336/347 (96.83%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MALFKL T+A  RTRTQSLLK LR MSNVPENTVYGGP SQ+PNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSAAMVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QA+DTAVRILK+GGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NI SAV+VVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+AL +YRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D NGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of Cla97C09G171880 vs. NCBI nr
Match: XP_008447278.1 (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 635.6 bits (1638), Expect = 2.6e-178
Identity = 324/347 (93.37%), Postives = 334/347 (96.25%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MALFKL T+AA RTR QSLLK LR MSNVPENTVY GP SQ+PNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSAAMVVHGHDTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QAVDTAVRILK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NI SAV+VVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+AL +YRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSN SFPGP HSPYKISD DLNGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of Cla97C09G171880 vs. NCBI nr
Match: KAG6575974.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 627.9 bits (1618), Expect = 5.3e-176
Identity = 317/347 (91.35%), Postives = 335/347 (96.54%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MALF L T AA R+ +QSLL  LR MSNVPENTVYGGP S +PNQRVTLTNL+QKYKKGE
Sbjct: 117 MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 176

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSA+MVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 177 PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 236

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 237 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 296

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NIASAV+VVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 297 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 356

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+ALSDY+E
Sbjct: 357 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 416

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISDDD NGFLKEL++MGLD AASAAAAKANR
Sbjct: 417 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 463

[Close]
BLAST of Cla97C09G171880 vs. NCBI nr
Match: XP_022953648.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita moschata] >XP_023547697.1 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.9 bits (1618), Expect = 5.3e-176
Identity = 317/347 (91.35%), Postives = 335/347 (96.54%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MALF L T AA R+ +QSLL  LR MSNVPENTVYGGP S +PNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSA+MVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NIASAV+VVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+ALSDY+E
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISDDD NGFLKEL++MGLD AASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

[Close]
BLAST of Cla97C09G171880 vs. ExPASy Swiss-Prot
Match: Q9M315 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.3e-153
Identity = 270/334 (80.84%), Postives = 305/334 (91.32%), Query Frame = 0 

Query: 14  TRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    +  +R MSNVPE+TVYGGPK Q+ NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGIDV LVGDSAAMVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LK+GGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPF 253
           GGFRPQG+NIASAV+VVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYREEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VIN+AL +Y+EEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           PYKIS  DL+GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of Cla97C09G171880 vs. ExPASy Swiss-Prot
Match: O82357 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 3.0e-153
Identity = 268/323 (82.97%), Postives = 302/323 (93.50%), Query Frame = 0 

Query: 23  LRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGID 82
           +R MSN+PENTVYGGPK Q+PNQRVTLT+LRQK+++GEPIT+VTAYDYPS VHLD AGID
Sbjct: 18  VRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGID 77

Query: 83  VALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVD 142
           V LVGDSA+MVVHGHDTTLPISLDEMLVHCRAVARGA+RPLLVGDLPFGTYESSS QAVD
Sbjct: 78  VCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAVD 137

Query: 143 TAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGRN 202
           TAVR+LK+GGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVLGGFRPQGRN
Sbjct: 138 TAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGRN 197

Query: 203 IASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQVLVYH 262
           IASAV+VVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPT+GIGAGPFCSGQVLVYH
Sbjct: 198 IASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVYH 257

Query: 263 DLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYREEVSNGSFPGPLHSPYKISDDDL 322
           DLLGM+QHPHHAKVTPKFCKQ+A +G+VIN+AL +Y+EEVS   FPGP HSPYKI+  +L
Sbjct: 258 DLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASEL 317

Query: 323 NGFLKELQNMGLDEAASAAAAKA 346
           +GFL ELQ +G D+AASAAA  A
Sbjct: 318 DGFLTELQKLGFDKAASAAALAA 340

[Close]
BLAST of Cla97C09G171880 vs. ExPASy Swiss-Prot
Match: Q9AWZ8 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 3.2e-139
Identity = 250/334 (74.85%), Postives = 287/334 (85.93%), Query Frame = 0 

Query: 24  RNMSNVPENTVYGGPKSQ------------SPNQRVTLTNLRQKYKKGEPITMVTAYDYP 83
           R +S+VPE+ VYGGP+ Q               +RVT+T LR K+++GEPIT+VTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 84  SGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFG 143
           S VH+D AGIDV LVGDSAAMVVHGHDTTLPISLD ML HCRAVARGA RPLLVGDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 144 TYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAIS 203
            YESSS +AVD+AVR+LK+GGMDAIKLEGGA SRI+AA+AIVEAGIAVMGHVGLTPQAIS
Sbjct: 138 CYESSSTRAVDSAVRVLKEGGMDAIKLEGGAPSRISAAKAIVEAGIAVMGHVGLTPQAIS 197

Query: 204 VLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAG 263
           VLGGFRPQG+ + SAV+VVETA++LQEAGCF+VVLECVP  VAAAAT+AL+IPT+GIGAG
Sbjct: 198 VLGGFRPQGKTVDSAVKVVETALALQEAGCFSVVLECVPAPVAAAATSALQIPTIGIGAG 257

Query: 264 PFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYREEVSNGSFPGPL 323
           PFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQF  +G VIN+ALS+Y++EV   SFPGP 
Sbjct: 258 PFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQFGNVGHVINKALSEYKQEVETRSFPGPS 317

Query: 324 HSPYKISDDDLNGFLKELQNMGLDEAASAAAAKA 346
           H+PYKI+  D++GF   LQ MGLDEAA+AAAA A
Sbjct: 318 HTPYKIAAADVDGFANALQKMGLDEAANAAAAAA 351

[Close]
BLAST of Cla97C09G171880 vs. ExPASy Swiss-Prot
Match: Q9AWZ7 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 5.6e-120
Identity = 221/326 (67.79%), Postives = 264/326 (80.98%), Query Frame = 0 

Query: 24  RNMSN-VPENTVYGG------PKSQSPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHL 83
           R MSN   E  +YGG        S +  +RVTL  LR K+++GEPI+MVTAYDYPSGVH+
Sbjct: 54  RAMSNGAAEPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHV 113

Query: 84  DMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESS 143
           D AG D+ LVGDSAAMV HGHD TLPISLD M+ HCRAVARGA R  LVGDLPFG+YE+S
Sbjct: 114 DAAGFDICLVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEAS 173

Query: 144 SVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGF 203
           + QAV +AVR++K+GG+++IKLEG A SRI+AARAIV+AGIAVMGH+GLTPQ++S LGGF
Sbjct: 174 TAQAVGSAVRVMKEGGVNSIKLEGSAPSRISAARAIVDAGIAVMGHIGLTPQSVSALGGF 233

Query: 204 RPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSG 263
           RPQG+ + SAV+VVE A++LQEAGCFAVVLECVP  VAAAAT+AL IPT+GIGAGPFCSG
Sbjct: 234 RPQGKTVESAVKVVEAALALQEAGCFAVVLECVPAPVAAAATSALTIPTIGIGAGPFCSG 293

Query: 264 QVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYREEVSNGSFPGPLHSPYK 323
           QVLVYHDLLG  Q   HAKV+PKFCKQ+  IGDVINRALS Y++EV   SFPGP H+PYK
Sbjct: 294 QVLVYHDLLGTFQ-TSHAKVSPKFCKQYGNIGDVINRALSKYKQEVETQSFPGPSHTPYK 353

Query: 324 ISDDDLNGFLKELQNMGLDEAASAAA 343
           ++  D++ FL  L+  GL+ AA AAA
Sbjct: 354 LAATDVDAFLNALKMKGLNVAADAAA 378

[Close]
BLAST of Cla97C09G171880 vs. ExPASy Swiss-Prot
Match: B0TC10 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=panB PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.1e-70
Identity = 136/280 (48.57%), Postives = 196/280 (70.00%), Query Frame = 0 

Query: 45  QRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPIS 104
           +++TL   ++  K+G+ + M+TAYDYP    +D + I++ LVGDS  MVV G+D+T+P++
Sbjct: 3   KKITLPQCKEMKKQGKRLRMITAYDYPFARLVDESEIEIILVGDSLGMVVLGYDSTVPVT 62

Query: 105 LDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHS 164
           LDEM+ HC+ V RGA   L+V D+PFG+Y  S   A+  A R+LK+ G++A+K+EGG   
Sbjct: 63  LDEMIHHCKPVVRGAPNTLVVADMPFGSYNVSKEDAIRNANRMLKESGIEAVKVEGGTRM 122

Query: 165 RITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAV 224
             T  RA+V+AGI VMGH+GLTPQ  + LGGF+ QG+   +A +++E A++L+ AG F++
Sbjct: 123 APT-VRALVDAGIPVMGHIGLTPQTAAQLGGFKVQGKTEDAAQQLLEDALALESAGAFSI 182

Query: 225 VLECVPPEVAAAATAALRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQF 284
           V+ECVP  +A   TA+L IPT+GIGAGP+C+GQVLV  DLLG+     + +  PKF KQ+
Sbjct: 183 VIECVPAGLARTITASLSIPTIGIGAGPYCNGQVLVIQDLLGI-----YDRFVPKFVKQY 242

Query: 285 AQIGDVINRALSDYREEVSNGSFPGPLHSPYKISDDDLNG 325
           AQ G  I  AL+DY  EV++G FPGP HS     DD++ G
Sbjct: 243 AQTGPAIRAALNDYAREVADGVFPGPEHS--FGMDDEMKG 274

[Close]
BLAST of Cla97C09G171880 vs. ExPASy TrEMBL
Match: A0A0A0K8B2 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.6e-180
Identity = 323/347 (93.08%), Postives = 336/347 (96.83%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MALFKL T+A  RTRTQSLLK LR MSNVPENTVYGGP SQ+PNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSAAMVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QA+DTAVRILK+GGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NI SAV+VVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+AL +YRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D NGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of Cla97C09G171880 vs. ExPASy TrEMBL
Match: A0A1S3BH28 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103489751 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 324/347 (93.37%), Postives = 334/347 (96.25%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MALFKL T+AA RTR QSLLK LR MSNVPENTVY GP SQ+PNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSAAMVVHGHDTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QAVDTAVRILK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NI SAV+VVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+AL +YRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSN SFPGP HSPYKISD DLNGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of Cla97C09G171880 vs. ExPASy TrEMBL
Match: A0A6J1GNW3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456114 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.6e-176
Identity = 317/347 (91.35%), Postives = 335/347 (96.54%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MALF L T AA R+ +QSLL  LR MSNVPENTVYGGP S +PNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSA+MVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NIASAV+VVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+ALSDY+E
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISDDD NGFLKEL++MGLD AASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

[Close]
BLAST of Cla97C09G171880 vs. ExPASy TrEMBL
Match: A0A6J1JXR6 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488459 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 9.8e-176
Identity = 316/347 (91.07%), Postives = 335/347 (96.54%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           MALF L T AA R+ +QSLL  LR MSNVPENTVYGGP S +PNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGID+ALVGDSA+MVVHGHDTTLPI+LDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LK+GGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NIASAV+VVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVIN+ALSDY+E
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD+D NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

[Close]
BLAST of Cla97C09G171880 vs. ExPASy TrEMBL
Match: A0A6J1D0E3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111016428 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.1e-174
Identity = 313/343 (91.25%), Postives = 329/343 (95.92%), Query Frame = 0 

Query: 1   MALFKLFTVAAPRTRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGE 60
           M LF  FT+A  RTRT+SLLK LR MSNVPENTVYGGPK Q P QRVTLTNLRQK+KKGE
Sbjct: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGID+ALVGDSA+MVVHGHDTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLVGDLPFGTYESSSVQAVDTAVRILK+GGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQG+NIASAV+VVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVINRAL DY+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDLNGFLKELQNMGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKIS+DD NGFLKELQN+GLDEAASAAAA
Sbjct: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 343

[Close]
BLAST of Cla97C09G171880 vs. TAIR 10
Match: AT3G61530.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 543.9 bits (1400), Expect = 9.4e-155
Identity = 270/334 (80.84%), Postives = 305/334 (91.32%), Query Frame = 0 

Query: 14  TRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    +  +R MSNVPE+TVYGGPK Q+ NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGIDV LVGDSAAMVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LK+GGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPF 253
           GGFRPQG+NIASAV+VVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYREEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VIN+AL +Y+EEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           PYKIS  DL+GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of Cla97C09G171880 vs. TAIR 10
Match: AT3G61530.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 543.9 bits (1400), Expect = 9.4e-155
Identity = 270/334 (80.84%), Postives = 305/334 (91.32%), Query Frame = 0 

Query: 14  TRTQSLLKVLRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    +  +R MSNVPE+TVYGGPK Q+ NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDVALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGIDV LVGDSAAMVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LK+GGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGRNIASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPF 253
           GGFRPQG+NIASAV+VVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYREEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VIN+AL +Y+EEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDDDLNGFLKELQNMGLDEAASAAAAKANR 348
           PYKIS  DL+GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of Cla97C09G171880 vs. TAIR 10
Match: AT2G46110.1 (ketopantoate hydroxymethyltransferase 1 )

HSP 1 Score: 542.7 bits (1397), Expect = 2.1e-154
Identity = 268/323 (82.97%), Postives = 302/323 (93.50%), Query Frame = 0 

Query: 23  LRNMSNVPENTVYGGPKSQSPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGID 82
           +R MSN+PENTVYGGPK Q+PNQRVTLT+LRQK+++GEPIT+VTAYDYPS VHLD AGID
Sbjct: 18  VRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGID 77

Query: 83  VALVGDSAAMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVD 142
           V LVGDSA+MVVHGHDTTLPISLDEMLVHCRAVARGA+RPLLVGDLPFGTYESSS QAVD
Sbjct: 78  VCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAVD 137

Query: 143 TAVRILKDGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGRN 202
           TAVR+LK+GGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVLGGFRPQGRN
Sbjct: 138 TAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGRN 197

Query: 203 IASAVEVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQVLVYH 262
           IASAV+VVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPT+GIGAGPFCSGQVLVYH
Sbjct: 198 IASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVYH 257

Query: 263 DLLGMLQHPHHAKVTPKFCKQFAQIGDVINRALSDYREEVSNGSFPGPLHSPYKISDDDL 322
           DLLGM+QHPHHAKVTPKFCKQ+A +G+VIN+AL +Y+EEVS   FPGP HSPYKI+  +L
Sbjct: 258 DLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASEL 317

Query: 323 NGFLKELQNMGLDEAASAAAAKA 346
           +GFL ELQ +G D+AASAAA  A
Sbjct: 318 DGFLTELQKLGFDKAASAAALAA 340

The following BLAST results are available for this feature:
BLAST of Cla97C09G171880 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038898896.17.9e-18093.373-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincas... [more]
XP_004139651.11.4e-17993.083-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativ... [more]
XP_008447278.12.6e-17893.37PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-lik... [more]
KAG6575974.15.3e-17691.353-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucu... [more]
XP_022953648.15.3e-17691.353-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbit... [more]
BLAST of Cla97C09G171880 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M3151.3e-15380.843-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis... [more]
O823573.0e-15382.973-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis... [more]
Q9AWZ83.2e-13974.853-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ... [more]
Q9AWZ75.6e-12067.793-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ... [more]
B0TC101.1e-7048.573-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum... [more]
BLAST of Cla97C09G171880 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K8B26.6e-18093.083-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3BH281.2e-17893.373-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1GNW32.6e-17691.353-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1JXR69.8e-17691.073-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1D0E34.1e-17491.253-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 ... [more]
BLAST of Cla97C09G171880 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G61530.19.4e-15580.84Phosphoenolpyruvate carboxylase family protein [more]
AT3G61530.29.4e-15580.84Phosphoenolpyruvate carboxylase family protein [more]
AT2G46110.12.1e-15482.97ketopantoate hydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003700Ketopantoate hydroxymethyltransferaseTIGRFAMTIGR00222TIGR00222coord: 48..313
e-value: 6.7E-86
score: 286.0
IPR003700Ketopantoate hydroxymethyltransferasePFAMPF02548Pantoate_transfcoord: 46..309
e-value: 8.5E-106
score: 353.1
IPR003700Ketopantoate hydroxymethyltransferasePANTHERPTHR208813-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 20..346
IPR003700Ketopantoate hydroxymethyltransferaseHAMAPMF_00156PanBcoord: 47..314
score: 39.826233
IPR003700Ketopantoate hydroxymethyltransferaseCDDcd06557KPHMT-likecoord: 49..308
e-value: 1.85388E-142
score: 401.412
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 44..325
e-value: 5.8E-119
score: 398.4
NoneNo IPR availablePIRSRPIRSR000388-1PIRSR000388-1coord: 55..309
e-value: 4.5E-88
score: 292.6
NoneNo IPR availablePANTHERPTHR20881:SF13-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 20..346
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 46..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C09G171880.1Cla97C09G171880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0015940 pantothenate biosynthetic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003864 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003824 catalytic activity